Leica M205 C, M165 C, M205 A User Manual

Leica M125

Leica M165 C

Leica M205 C

Leica M205 A

Manual

General Instructions

Safety concept

Before using your microscope for the first time, please read the "Safety concept" brochure included with your instrument. It contains additional information about handling and care.

Use in clean rooms

The Leica M series can be used in clean rooms without any problems.

Cleaning

Do not use any unsuitable cleaning agents, chemicals or techniques for cleaning.

Never use chemicals to clean colored surfaces or accessories with rubberized parts. This could damage the surfaces, and specimens could be contaminated by abraded particles.

In most cases, we can provide special solutions on request. Some products can be modified, and we can offer other accessories for use in clean rooms.

Servicing

Repairs may only be carried out by Leica Microsystems-trained service technicians. Only original Leica Microsystems spare parts may be used.

Responsibilities of person in charge of instrument

Ensure that the Leica stereomicroscope is operated, maintained and repaired by authorized and trained personnel only.

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Safety concept

The individual modules of the Leica M stereomicroscopy series include an interactive CD-ROM with all relevant user manuals in 20 other languages. Keep it in a safe place, and readily accessible to the user. User manuals and updates are also available for you to download and print from our web site at www.stereomicroscopy.com.

This operating manual describes the special functions of the individual modules of the Leica M stereomicroscopy series and contains important instructions for their operational safety, maintenance, and accessories.

The "Safety concept" booklet contains additional safety information regarding the service work, requirements and the handling of stereomicroscope, accessories and electrical accessories as well as general safety instructions.

You can combine individual system articles with articles from external suppliers (e.g. cold light sources, etc.). Please read the user manual and the safety requirements of the supplier.

Before installing, operating or using the instruments, read the user manuals listed above. In particular, please observe all safety instructions.

To maintain the unit in its original condition and to ensure safe operation, the user must follow the instructions and warnings contained in these user manuals.

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Symbols used

Warning of a danger

•This symbol indicates especially important information that must be read and

complied with. Failure to comply can cause the following:

Hazards to personnel

Functional disturbances or damaged instruments

Warning of hazardous electrical voltage

This symbol indicates especially important information that, if not observed,

can cause the following:

Hazards to personnel

Functional disturbances or damaged instruments.

Danger due to hot surface

This symbol warns against touching hot surfaces, e.g. those of light bulbs.

Important information

This symbol indicates additional information or explanations that intend to

provide clarity.

Action

This symbol refers to actions described in the text that are to be carried out.

Figures

(1)Numbers in brackets within the descriptions relate to the figures and the items within those figures.

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Safety regulations

Description

The individual modules fulfill the highest requirements for observation and documentation of Leica stereo microscopes of the M series.

Designated use

See “Safety concept” booklet

Non-intended use

See “Safety concept” booklet

Never use M series microscopes or their components for surgical procedures (e.g. on the eye) unless they are specifically intended for that purpose.

The devices and accessories described in this operating manual have been tested for safety and potential hazards. The responsible Leica affiliate must be consulted whenever the device is altered, modified or used in conjunction with

non-Leica components that are outside of the scope of this manual.

Unauthorized alterations to the instrument or noncompliant use shall void all rights to any warranty claims!

Location of use

See “Safety concept” booklet

Set up the electrical components at least 10 cm from walls and combustible objects.

Avoid large temperature fluctuations, direct sunlight and vibrations. These conditions can distort measurements and micrographic images.

In warm and warm-damp climatic zones, the individual components require special care in order to prevent the build up of fungus.

Requirements for the owner/operator

See “Safety concept” booklet

Ensure that:

The M series stereomicroscopes and accessories are operated, maintained and repaired by authorized and trained personnel only.

All operators have read, understood and observe this user manual, and particularly the safety instructions.

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Safety regulations (continued)

Repair, service work		Installation in third-party products	Health risks
	See “Safety concept” booklet	See “Safety concept” booklet	Workplaces equipped with stereomicroscopes
			facilitate and improve the viewing task, but
Only original Leica Microsystems spare			they also impose high demands on the eyes
	parts may be used.	Disposal	and holding muscles of the user. Depending on
		See “Safety concept” booklet	the duration of uninterrupted work, asthenopia
Before opening the instruments, switch off			and musculoskeletal problems may occur. For
	the power and unplug the power cable.		this reason, appropriate measures for reduction
		Legal regulations	of the workload must be taken:
Touching the live circuit can cause injury.		See “Safety concept” booklet
			Optimal arrangement of workplace, work
			assignments and work flow (changing tasks
Transport		EC Declaration of Conformity	frequently).
	Use the original packaging for shipping or	See “Safety concept” booklet
	transporting the individual modules of the		Thorough training of the personnel, giving
	Leica M stereomicroscopy series and the		consideration to ergonomic and organiza-
	accessory components.		tional aspects.
In order to prevent damage from vibrations,			The ergonomic design and construction of the
	disassemble all moving parts that (accord-		Leica M stereomicroscopy series are intended to
	ing to the user manual) can be assembled		reduce the exertion of the user to a minimum.
	and disassembled by the customer and
	pack them separately.

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Safety regulations (continued)

Direct contact with eyepieces can be a potential transmission path for bacterial and viral infections of the eye.

The risk can be kept to a minimum by using personal eyepieces for each individual or detachable eyecups.

Light sources: safety instructions		Warning
Protective measures of the manufacturer:		UV radiation could damage the eyes. Therefore:
	UV protection screen in front of the speci-	Never look into the light spot on the speci-
	men plane prevents the user from looking	men plane without a UV protection screen.
	directly into the UV rays.
		Never look into the eyepieces if no excita-
	Dummy filter carriers in the free positions	tion filter is in the beam path.
	of the rapid filter changer prevent direct UV
	radiation from reaching the eyes.	Fill empty filter positions with dummy filter
		carriers (e.g. M165FC).
	UV filters are installed in the observation
	beam paths to protect the eyes.	Do not select a white, strongly reflective
		background for the stage.
The stray-light protection on the lamp
	housing prevents irradiation of the hands.

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Safety regulations (continued)

Supply unit

Always unplug the supply unit from the power supply:

When installing and disassembling the lamp housing

Before opening the lamp housing

When replacing the high-pressure mercury lamp and other parts, such as the heat-ab- sorbing filter or the collector

during maintenance work on the supply unit.

Lamp housing

Never open the lamp housing while the lamp is switched on. Risk of explosion, UV exposure, blinding!

Before opening the lamp housing, allow it to cool off for at least 15 minutes. Danger of explosion!

Never cover the air duct on the lamp housing. Danger of fire!

Mercury lamp

Follow the user manual and safety instructions provided by the lamp manufacturer, and, in particular, the section on how to proceed if a lamp breaks and releases mercury.

For transport, remove the mercury lamp, transport it in its original packaging and protect moving parts in the lamp housing using the transport anchors.

To minimize the risk of explosion, replace a discoloured mercury lamp promptly when it has reached the end of its rated life (follow the manufacturer's specifications and the minute meter on the supply unit).

Leica Microsystems assumes no liability for damage caused by exploding, incorrectly installed or improperly used mercury lamps.

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Table of Contents

General Instructions	2
Safety concept	3
Symbols used	4
Safety regulations	5
The Leica M Series
Congratulations!	13
A Step Towards Infinity	14
The Electronics: Comfort, Convenience and Safety	15
The Modular Design: Everything is Relative	16
Maximum Compatibility	17
On We Go	18
Assembly
Base and Focusing Column	20
Optics carrier	21
Tube	22
Eyepieces	23
Objective	24
Objective Nosepiece – Assembly	25
Objective Nosepiece – Adjusting Parfocality	26
AX Carrier – Preparation	27
AX Carrier – Assembly	28
Transmitted-light Base TL ST	29

Transmitted-light Base TL BFDF: Before First Use	30
Transmitted-light Base TL BFDF	31
TL RC™ / TL RCI™	32
IsoPro™ Manual Cross-stage: Assembly	33
IsoPro™ Motorized Cross-stage: Assembly	36
Cables: Terminals	40
Cables: Cable Duct	41
Cables: Diagram	42
Leica LED5000 MCI™	43
Leica LED5000 MCI™: Alternative Assembly	44
Leica LED5000 RL	45
Quick Start Guide
The Fastest Route to Success	47
Overview of an M series Microscope	48
The Correct Interpupillary Distance	49
Using the Eyepieces	50
Focusing	51
Adjusting the resistance of the focus drive	52
Changing the magnification (zoom)	53
Ratchet Steps and Magnification Levels	54
Parfocality: More Comfort and Convenience for Your Work	55
Iris Diaphragm	56

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Eyepieces
Magnification Factors of the Eyepieces	58
Health Notes	59
Dioptric correction	60
Dioptric Correction and Parfocality	61
Graticules	62
Photography & Video
Photography & Video	64
Photo tubes and C-mounts	65
Trinocular video/phototube 50%	66
Trinocular video/phototube 100%	67
Microscope Carrier
The Microscope Carrier AX	69
The Objective Nosepiece	71
Objectives andoptical accessories
The Different Types of Objectives	73

Bases
Transmitted-light Base TL ST: Controls	75
Transmitted-light Base TL ST: Operation	76
Transmitted-light Base TL ST: Lamp Replacement	77
Transmitted-light Base TL BFDF: Controls	78
Transmitted-light Base TL BFDF: Operation	79
TL RC™ / TL RCI™: Controls	80
TL RCI™: The Deflection Mirror	81
TL RCI™: Color Intensity and Temperature	82
TL RC™ / TL RCI™: Operation	83
TL RCI™: Methods in Transmitted Light	84
TL RCI™: Relief Images	85
Using Filters	87
IsoPro™ (Non-motorized): Controls	88
IsoPro™ (Motorized): Controls	89
System Illumination
Leica LED5000 MCI™	91
Leica LED5000 RL	93

Leica M series	Manual	10

Accessories
Leica PSC Controller	95
Dimensional Drawings
Leica M125 / M165 C	97
Leica M205 C	101
Leica LED5000 MCI	104
Leica LED5000 RL	105
Specifications for the Bases
Transmitted-light Base TL ST	107
Transmitted-light Base TL BFDF	108
TL RC™ / TL RCI™	109
IsoPro™ Motorized XY Stage	110
Appendix
Calculating the Total Magnification and Field of View Diameter	112
Care, Maintenance, Contact Persons	113

Leica M series	Manual	11

The Leica M Series

Leica M series	Manual	12

Congratulations!

Congratulations on obtaining your new Leica M series stereomicroscope. We are convinced it will exceed your expectations, as never before have we applied our decades of experience in the areas of optics, mechanical engineering and ergonomics in such an uncompromising manner.

The M series embodies all the qualities you associate with the name Leica Microsystems: excellent objectives, high-quality engineering, and reliability. Furthermore, the modular design ensures that the M series adapts perfectly to your needs—no matter which accessories you require for your tasks.

Though the reliability and robustness of Leica stereomicroscopes is legendary, like any high-tech product, the M series requires a certain degree of care and attention. Therefore, we recommend that you read this manual. It contains all the information you need regarding operation, safety and maintenance. Simply observing a few guidelines will ensure that even after years of intensive use, your stereomicroscope will continue to work as smoothly and reliably as on the very first day.

We wish you the best of success in your work—after all, you are now equipped with the best tool!

Leica M series	Manual	13

A Step Towards Infinity

Ever since their introduction by Horatio S. Greenough, stereomicroscopes have worked according to the optical principles based primarily on Ernst Abbe's research. For over a century, ingenious optics designers and engineers have worked to push magnification, resolution and image fidelity to the limit permitted by optics.

In doing so, they have always been constrained by the interrelation between three factors: the higher a microscope's resolution, the lower the available working distance. If one increases the distance of the optical axes, the three-dimen- sional image seen by the observer becomes distorted—a sphere becomes an ellipse, a flat surface curves toward the observer.

Limits are made to be broken.

The Leica M205 C is the world's first stereomicroscope with a zoom range of 20.5:1. This accomplishment, however, was not enough for Leica's engineers. With the new FusionOptic™ in the M205 C, they have succeeded in going yet another step beyond previous limits. In addition to the increase in magnification, the resolution, too, has been increased to up to 1050 lp / mm*, which corresponds to a resolved structure size of 472 nm.

Of course, this performance increase benefits your everyday work. Set up your specimens on the microscope with comfortable freedom of movement and discover details in stereomicroscopy that you could never see before.

* Leica M205 C with 1.0× planapochromat and 10× eyepieces

Leica M series	Manual	14

The Electronics: Comfort, Convenience and Safety for your Experiments

Never before have electronics been used as extensively in a Leica series as in the new M series. Optics carrier, tripod, base and illuminator are all connected using electrical contacts—which provides a number of advantages.

Reliability for your experiments

Exact reproducibility of results is an increasingly common demand, particularly in research and development. The continuous encoding captures parameters such as the magnification, the illumination, the position of the iris

diaphragm and more to the Leica LAS software. Thus you always know the conditions under which an image was acquired.

Fewer cables

A large part of the cables have been routed in the interior of the column. Data are also transferred via the interface between the column, the optics carrier and the carrier. The direct result is that you need fewer cables—this not only makes your workstation neater and more comfortable, it even makes it appear larger.

Contacts not only transmit data, but also supply the power.

Leica Application Suite (LAS) evaluates the transmitted data and can restore the test situation later.

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The Modular Design: Everything is Relative

The Leica M series provides maximum flexibility in choosing equipment, thanks primarily to the modular configuration and the compatibility that Leica has painstakingly maintained for decades. The optics carriers, eyepieces, bases and more can be combined in any way you choose, allowing you to create the microscope that best suits your needs.

Despite this, you will notice that the controls and individual components do not differ significantly. Whichever configuration you choose, you will quickly feel right at home.

Have a special request? Let us know!

Leica Microsystems enjoys an exceptional reputation when it comes to devising customerspecific solutions. If you have a special request that cannot be met with standard parts, contact your Leica consultant. We have a solution for every problem.

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Maximum Compatibility

Leica engineers were careful to ensure that the new M series—like its predecessors—remains compatible with existing series. This means that objectives, bases, tubes and so on can be reused.

Objectives

All new objectives of the M series are parfocal, meaning that when used with the objective nosepiece, they can be replaced while keeping the specimen in perfect focus.

If you prefer, you can continue to use the previous objective series. In this case, parfocality is no longer guaranteed.

Tubes

The interface between the optics carrier and the tube has remained the same, so existing tubes fit the new M series. The new tubes are designed for eyepieces with field number 23, while the predecessor models were only designed for field number 21, resulting in a smaller object field.

Eyepieces

However, the new M-series eyepieces models have an audible and tangible click to provide immediate feedback in case of accidental adjustment.

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On We Go

If your new Leica microscope has already been assembled and commissioned by your Leica consultant, click here to skip through the installation instructions and go directly to the Quick Start Guide on page 46.

If, on the other hand, you are assembling the microscope yourself, continue with the "Assembly" chapter, which begins on page 19.

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Assembly

Leica M series	Manual	19

Base and Focusing Column

The first step is to connect the focusing column	Assembling the column adapter	Assembling the focusing drive
of the M series to the corresponding base.	1. Securely install the column adapter on the	2. Securely screw the focusing column to the
	column using the four included screws.	base using the six included screws.
Tools used
Hex socket screwdriver, 3 mm

Leica M series	Manual	20

Optics carrier

Tools used	Assembling the optics carrier
Hex socket screwdriver, 4 mm	1. Place the optics carrier on the focusing
	column so that the screw fit into the thread
	provided and the lug fits into the groove.

2.Press the optics carrier backwards to the focusing column and screw it in place using your other hand.

Leica M series	Manual	21

Tube

All intermediate tubes that fit between the optics carrier and the binocular tube are fitted in the same manner.

Tools used

No tools required.

Preparations

1.Unscrew the positioning screw and remove the protective cover.

Assembling the tube

2.Push the tube (for example, the inclined binocular tube) into the dovetail ring and rotate it slightly in both directions until the positioning screw meshes with the guide groove.

3. While holding the tube only slightly, carefully tighten the positioning screw. It is automatically brought to the correct position.

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Eyepieces

Tools used	Preparation				Inserting the eyepieces
No tools required.	1.	If you want to use an optional graticule,			3. Push the eyepieces into the tubes as far as
		insert it now (page 62).			they will go and check to ensure that they
Magnification range					fit tightly and accurately.
You can extend the overall magnification range	2.	Remove the plastic tube guard.
using available 10×, 16×, 25× and 40× wide-
field eyepieces for persons wearing glasses.

4. Securely tighten the clamping screws.

Leica M series	Manual	23

Objective

Tools used	Attaching the objective	Alternative fastening options
No tools required.	Hold the objective firmly during assem-	If using the objective nosepiece, read the
	• bly and disassembly so that it does not	instructions on page 25.
	fall onto the stage plate. This applies particu-
Preparation	larly to the 2× planapochromatic objective,	If using the AX carrier, read the instructions
1. Remove the protective cap on the optics	which is very heavy. Remove all specimens from	on page 27.
carrier by turning it.	the stage plate first.
	2. Screw the objective clockwise into the
	optics carrier.

Leica M series	Manual	24

Objective Nosepiece – Assembly

Preparations

• Hold the objectives firmly during assembly and disassembly so that they

do not fall onto the stage plate.

Move the drive housing all the way upwards and remove the optics carrier, if the carrier has already been installed.

Assembly

1.Remove the transport anchor from the objective nosepiece.

2.Rotate the moving part by 90° and attach the objective nosepiece to the drive housing from the front (!). Screw the objective nosepiece firmly into place.

3.Unscrew the three Phillips screws on the objective mount of the optics carrier and remove the intermediate ring.

4.Screw the optics carrier onto the objective nosepiece.

5.Screw both objectives onto the objective nosepiece. It makes no difference which position an objective occupies.

6.Unscrew the locking screws on both sides of the objective nosepiece.

You can now adjust the parfocality (see instructions on the next page).

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Objective Nosepiece – Adjusting Parfocality

The following procedure only has to be carried out once. Afterwards, both objectives are parfocal, meaning that the specimen remains in focus when the objective nosepiece is rotated.

This procedure must be repeated if you replace either of the two objectives with another.

The following example assumes the combination of the 1× and 2× planapochromats. If you are using another objective combination, replace the 2× objective in the description with the objective with the stronger magnification.

Preparation

Open the iris diaphragm.

Set the dioptric correction of the eyepieces to "0".

Adjustment

1.Rotate the 2× objective into the beam path and set it to the lowest magnification.

2.Focus on the specimen.

3.Rotate the 1× objective into the beam path.

4.Turn the objective on the thread in both directions until the specimen appears sharp.

5.Toggle to the 2× objective.

6.Select the strongest magnification and refocus until the specimen appears absolutely sharp.

7.Toggle to the 1× objective.

8.Turn the objective on the thread in both directions until the specimen appears absolutely sharp.

By means of zooming, check that the behavior of the objective is parfocal. Repeat the check with the other objective. If it is not parfocal, repeat the procedure.

9. Tighten the locking screws.

Leica M series	Manual	26

AX Carrier – Preparation

Tools

Hex socket screwdriver, 2.5 mm

Preparing the AX carrier

Before the AX carrier is installed, it may first have to be prepared for the optics carrier to be used (Leica M125, M205 C or M165 C).

1. Check the switch position.

If the switch position corresponds to the optics carrier used, skip the following steps. Otherwise, proceed as follows:

2.Unscrew the two screws that hold the switch in position.

3.Pull out the switch, rotate it into the desired position and reinsert it.

4.Important: push the switch all the way into the mount while reinserting the screws. Only this ensures that the switch is positioned correctly.

Leica M series	Manual	27

AX Carrier – Assembly

Tools

Hex socket screwdriver, 4 mmPhilips screwdriver

Once the AX carrier has prepared for the corresponding optics carrier, it can be installed.

1.Move the slide of the AX carrier into mid position.

2.Install the AX carrier on the focusing column.

3. Screw the objective to the AX carrier.

4.Unscrew the three holding screws and remove the adapter ring on the optics carrier.

5. Install the optics carrier on the AX carrier.

Wrong

Right

Leica M series	Manual	28

Transmitted-light Base TL ST

Unpacking the base	Focusing drive and column	2.	Attach your focusing drive column to the
The base is delivered with the adapter plate	1. Unscrew the extension plate from the base		bottom using the 4 Allen screws.
installed. Make sure the devices are unpacked	using the Allen key provided.
on a flat, sufficiently dimensioned, and non-slip		3.	Reattach the adapter plate to its original
surface.			position using the 6 Allen screws.

Leica M series	Manual	29

Transmitted-light Base TL BFDF: Before First Use

	Removing the transport anchors
	Before you can use the transmitted-
	• light base for the first time, it is abso-
	lutely necessary to remove the two transport	Anchor of mirror
	anchors as follows.

Anchor of switching slide

Leica M series	Manual	30