Leica M205 C, M165 C, M205 A User Manual
Leica M125
Leica M165 C
Leica M205 C
Leica M205 A
Manual
General Instructions
Safety concept
Before using your microscope for the first time,
please read the "Safety concept" brochure
included with your instrument. It contains additional information about handling and care.
Use in clean rooms
The Leica M series can be used in clean rooms
without any problems.
Cleaning
Do not use any unsuitable cleaning agents, ★
chemicals or techniques for cleaning.
Never use chemicals to clean colored ★
surfaces or accessories with rubberized
parts. This could damage the surfaces,
and specimens could be contaminated by
abraded particles.
In most cases, we can provide special solu- ★
tions on request. Some products can be
modified, and we can offer other accessories for use in clean rooms.
Servicing
Repairs may only be carried out by Leica ★
Microsystems-trained service technicians.
Only original Leica Microsystems spare
parts may be used.
Responsibilities of person in charge of
instrument
Ensure that the Leica stereomicroscope ★
is operated, maintained and repaired by
authorized and trained personnel only.
Leica M series Manual 2
Safety concept
The individual modules of the Leica M stereomicroscopy series include an interactive
CD-ROM with all relevant user manuals in 20
other languages. Keep it in a safe place, and
readily accessible to the user. User manuals and
updates are also available for you to download
and print from our web site at www.stereomicroscopy.com.
This operating manual describes the special
functions of the individual modules of the Leica
M stereomicroscopy series and contains important instructions for their operational safety,
maintenance, and accessories.
The "Safety concept" booklet contains additional safety information regarding the service
work, requirements and the handling of stereomicroscope, accessories and electrical accessories as well as general safety instructions.
You can combine individual system articles
with articles from external suppliers (e.g. cold
light sources, etc.). Please read the user manual
and the safety requirements of the supplier.
Before installing, operating or using the instruments, read the user manuals listed above. In
particular, please observe all safety instructions.
To maintain the unit in its original condition
and to ensure safe operation, the user must
follow the instructions and warnings contained
in these user manuals.
Leica M series Manual 3
Symbols used
Warning of a danger
This symbol indicates especially impor-
tant information that must be read and
complied with. Failure to comply can cause the
following:
Hazards to personnel ★
Functional disturbances or damaged instru- ★
ments
Warning of hazardous electrical voltage
This symbol indicates especially impor-
tant information that, if not observed,
can cause the following:
Hazards to personnel ★
Functional disturbances or damaged instru- ★
ments.
Danger due to hot surface
This symbol warns against touching hot
surfaces, e.g. those of light bulbs.
Important information
This symbol indicates additional information or explanations that intend to
provide clarity.
Action
This symbol refers to actions described in ★
the text that are to be carried out.
Figures
(1) Numbers in brackets within the descrip-
tions relate to the figures and the items
within those figures.
Leica M series Manual 4
Safety regulations
Description
The individual modules fulfill the highest
requirements for observation and documentation of Leica stereo microscopes of the
M series.
Designated use
See “Safety concept” booklet ★
Non-intended use
See “Safety concept” booklet ★
Never use M series microscopes or their components for surgical procedures (e.g. on the eye)
unless they are specifically intended for that
purpose.
The devices and accessories described in this
operating manual have been tested for safety
and potential hazards. The responsible Leica
affiliate must be consulted whenever the device
is altered, modified or used in conjunction with
non-Leica components that are outside of the
scope of this manual.
Unauthorized alterations to the instrument or
noncompliant use shall void all rights to any
warranty claims!
Location of use
See “Safety concept” booklet ★
Set up the electrical components at least 10 ★
cm from walls and combustible objects.
Avoid large temperature fluctuations, ★
direct sunlight and vibrations. These conditions can distort measurements and micrographic images.
In warm and warm-damp climatic zones, ★
the individual components require special
care in order to prevent the build up of
fungus.
Requirements for the owner/operator
See “Safety concept” booklet ★
Ensure that:
The M series stereomicroscopes and ★
accessories are operated, maintained and
repaired by authorized and trained personnel only.
All operators have read, understood and ★
observe this user manual, and particularly
the safety instructions.
Leica M series Manual 5
Safety regulations (continued)
Repair, service work
See “Safety concept” booklet ★
Only original Leica Microsystems spare ★
parts may be used.
Before opening the instruments, switch off ★
the power and unplug the power cable.
Touching the live circuit can cause injury. ★
Transport
Use the original packaging for shipping or ★
transporting the individual modules of the
Leica M stereomicroscopy series and the
accessory components.
In order to prevent damage from vibrations, ★
disassemble all moving parts that (according to the user manual) can be assembled
and disassembled by the customer and
pack them separately.
Installation in third-party products
See “Safety concept” booklet ★
Disposal
See “Safety concept” booklet ★
Legal regulations
See “Safety concept” booklet ★
EC Declaration of Conformity
See “Safety concept” booklet ★
Health risks
Workplaces equipped with stereomicroscopes
facilitate and improve the viewing task, but
they also impose high demands on the eyes
and holding muscles of the user. Depending on
the duration of uninterrupted work, asthenopia
and musculoskeletal problems may occur. For
this reason, appropriate measures for reduction
of the workload must be taken:
Optimal arrangement of workplace, work ★
assignments and work flow (changing tasks
frequently).
Thorough training of the personnel, giving ★
consideration to ergonomic and organizational aspects.
The ergonomic design and construction of the
Leica M stereomicroscopy series are intended to
reduce the exertion of the user to a minimum.
Leica M series Manual 6
Safety regulations (continued)
Direct contact with eyepieces can be a potential
transmission path for bacterial and viral infections of the eye.
The risk can be kept to a minimum by using
personal eyepieces for each individual or
detachable eyecups.
Light sources: safety instructions
Protective measures of the manufacturer:
UV protection screen in front of the speci- ★
men plane prevents the user from looking
directly into the UV rays.
Dummy filter carriers in the free positions ★
of the rapid filter changer prevent direct UV
radiation from reaching the eyes.
UV filters are installed in the observation ★
beam paths to protect the eyes.
The stray-light protection on the lamp ★
housing prevents irradiation of the hands.
Warning
UV radiation could damage the eyes. Therefore:
Never look into the light spot on the speci- ★
men plane without a UV protection screen.
Never look into the eyepieces if no excita- ★
tion filter is in the beam path.
Fill empty filter positions with dummy filter ★
carriers (e.g. M165FC).
Do not select a white, strongly reflective ★
background for the stage.
Leica M series Manual 7
Safety regulations (continued)
Supply unit
Always unplug the supply unit from the power
supply:
When installing and disassembling the ★
lamp housing
Before opening the lamp housing ★
When replacing the high-pressure mercury ★
lamp and other parts, such as the heat-absorbing filter or the collector
during maintenance work on the supply ★
unit.
Lamp housing
Never open the lamp housing while the ★
lamp is switched on. Risk of explosion, UV
exposure, blinding!
Before opening the lamp housing, allow it ★
to cool off for at least 15 minutes. Danger
of explosion!
Never cover the air duct on the lamp hous- ★
ing. Danger of fire!
Mercury lamp
Follow the user manual and safety instruc- ★
tions provided by the lamp manufacturer,
and, in particular, the section on how to
proceed if a lamp breaks and releases
mercury.
For transport, remove the mercury lamp, ★
transport it in its original packaging and
protect moving parts in the lamp housing
using the transport anchors.
To minimize the risk of explosion, replace a ★
discoloured mercury lamp promptly when
it has reached the end of its rated life (follow
the manufacturer's specifications and the
minute meter on the supply unit).
Leica Microsystems assumes no liability ★
for damage caused by exploding, incorrectly installed or improperly used mercury
lamps.
Leica M series Manual 8
Table of Contents
General Instructions 2
Safety concept 3
Symbols used 4
Safety regulations 5
The Leica M Series
Congratulations! 13
A Step Towards Infinity 14
The Electronics: Comfort, Convenience and Safety 15
The Modular Design: Everything is Relative 16
Maximum Compatibility 17
On We Go 18
Assembly
Base and Focusing Column 20
Optics carrier 21
Tube 22
Eyepieces 23
Objective 24
Objective Nosepiece – Assembly 25
Objective Nosepiece – Adjusting Parfocality 26
AX Carrier – Preparation 27
AX Carrier – Assembly 28
Transmitted-light Base TL ST 29
Transmitted-light Base TL BFDF: Before First Use 30
Transmitted-light Base TL BFDF 31
TL RC™ / TL RCI™ 32
IsoPro™ Manual Cross-stage: Assembly 33
IsoPro™ Motorized Cross-stage: Assembly 36
Cables: Terminals 40
Cables: Cable Duct 41
Cables: Diagram 42
Leica LED5000 MCI™ 43
Leica LED5000 MCI™: Alternative Assembly 44
Leica LED5000 RL 45
Quick Start Guide
The Fastest Route to Success 47
Overview of an M series Microscope 48
The Correct Interpupillary Distance 49
Using the Eyepieces 50
Focusing 51
Adjusting the resistance of the focus drive 52
Changing the magnification (zoom) 53
Ratchet Steps and Magnification Levels 54
Parfocality: More Comfort and Convenience for Your Work 55
Iris Diaphragm 56
Leica M series Manual 9
Eyepieces
Magnification Factors of the Eyepieces 58
Health Notes 59
Dioptric correction 60
Dioptric Correction and Parfocality 61
Graticules 62
Photography & Video
Photography & Video 64
Photo tubes and C-mounts 65
Trinocular video/phototube 50% 66
Trinocular video/phototube 100% 67
Microscope Carrier
The Microscope Carrier AX 69
The Objective Nosepiece 71
Objectives andoptical accessories
The Different Types of Objectives 73
Bases
Transmitted-light Base TL ST: Controls 75
Transmitted-light Base TL ST: Operation 76
Transmitted-light Base TL ST: Lamp Replacement 77
Transmitted-light Base TL BFDF: Controls 78
Transmitted-light Base TL BFDF: Operation 79
TL RC™ / TL RCI™: Controls 80
TL RCI™: The Deflection Mirror 81
TL RCI™: Color Intensity and Temperature 82
TL RC™ / TL RCI™: Operation 83
TL RCI™: Methods in Transmitted Light 84
TL RCI™: Relief Images 85
Using Filters 87
IsoPro™ (Non-motorized): Controls 88
IsoPro™ (Motorized): Controls 89
System Illumination
Leica LED5000 MCI™ 91
Leica LED5000 RL 93
Leica M series Manual 10
Accessories
Leica PSC Controller 95
Dimensional Drawings
Leica M125 / M165 C 97
Leica M205 C 101
Leica LED5000 MCI 104
Leica LED5000 RL 105
Specications for the Bases
Transmitted-light Base TL ST 107
Transmitted-light Base TL BFDF 108
TL RC™ / TL RCI™ 109
IsoPro™ Motorized XY Stage 110
Appendix
Calculating the Total Magnification and Field of View Diameter 112
Care, Maintenance, Contact Persons 113
Leica M series Manual 11
The Leica M Series
Leica M series Manual 12
Congratulations!
Congratulations on obtaining your new Leica M series stereomicroscope.
We are convinced it will exceed your expectations, as never before have
we applied our decades of experience in the areas of optics, mechanical
engineering and ergonomics in such an uncompromising manner.
The M series embodies all the qualities you associate with the name Leica
Microsystems: excellent objectives, high-quality engineering, and reliability. Furthermore, the modular design ensures that the M series adapts
perfectly to your needs—no matter which accessories you require for your
tasks.
Though the reliability and robustness of Leica stereomicroscopes is
legendary, like any high-tech product, the M series requires a certain
degree of care and attention. Therefore, we recommend that you read
this manual. It contains all the information you need regarding operation,
safety and maintenance. Simply observing a few guidelines will ensure
that even after years of intensive use, your stereomicroscope will continue
to work as smoothly and reliably as on the very first day.
We wish you the best of success in your work—after all, you are now
equipped with the best tool!
Leica M series Manual 13
A Step Towards Infinity
Ever since their introduction by Horatio S.
Greenough, stereomicroscopes have worked
according to the optical principles based
primarily on Ernst Abbe's research. For over a
century, ingenious optics designers and engineers have worked to push magnification, resolution and image fidelity to the limit permitted
by optics.
In doing so, they have always been constrained
by the interrelation between three factors: the
higher a microscope's resolution, the lower the
available working distance. If one increases the
distance of the optical axes, the three-dimensional image seen by the observer becomes
distorted—a sphere becomes an ellipse, a flat
surface curves toward the observer.
Limits are made to be broken.
The Leica M205 C is the world's first stereomicroscope with a zoom range of 20.5:1. This
accomplishment, however, was not enough for
Leica's engineers. With the new FusionOptic™ in
the M205 C, they have succeeded in going yet
another step beyond previous limits. In addition
to the increase in magnification, the resolution,
too, has been increased to up to 1050 lp / mm*,
which corresponds to a resolved structure size
of 472 nm.
Of course, this performance increase benefits
your everyday work. Set up your specimens
on the microscope with comfortable freedom
of movement and discover details in stereomicroscopy that you could never see before.
* Leica M205 C with 1.0× planapochromat and 10× eyepieces
Leica M series Manual 14
The Electronics: Comfort, Convenience and Safety for your Experiments
Never before have electronics been used as
extensively in a Leica series as in the new
M series. Optics carrier, tripod, base and illuminator are all connected using electrical
contacts—which provides a number of advantages.
Contacts not only transmit data, but also supply
the power.
Reliability for your experiments
Exact reproducibility of results is an increasingly common demand, particularly in research
and development. The continuous encoding
captures parameters such as the magnification, the illumination, the position of the iris
Leica Application Suite (LAS) evaluates the
transmitted data and can restore the test situation later.
diaphragm and more to the Leica LAS software.
Thus you always know the conditions under
which an image was acquired.
Fewer cables
A large part of the cables have been routed in
the interior of the column. Data are also transferred via the interface between the column,
the optics carrier and the carrier. The direct
result is that you need fewer cables—this not
only makes your workstation neater and more
comfortable, it even makes it appear larger.
Leica M series Manual 15
The Modular Design: Everything is Relative
The Leica M series provides maximum flexibility in choosing equipment, thanks primarily to
the modular configuration and the compatibility that Leica has painstakingly maintained for
decades. The optics carriers, eyepieces, bases
and more can be combined in any way you
choose, allowing you to create the microscope
that best suits your needs.
Despite this, you will notice that the controls
and individual components do not differ significantly. Whichever configuration you choose,
you will quickly feel right at home.
Have a special request? Let us know!
Leica Microsystems enjoys an exceptional reputation when it comes to devising customerspecific solutions. If you have a special request
that cannot be met with standard parts, contact
your Leica consultant. We have a solution for
every problem.
Leica M series Manual 16
Maximum Compatibility
Leica engineers were careful to ensure that the
new M series—like its predecessors—remains
compatible with existing series. This means
that objectives, bases, tubes and so on can be
reused.
Objectives
All new objectives of the M series are parfocal,
meaning that when used with the objective
nosepiece, they can be replaced while keeping
the specimen in perfect focus.
If you prefer, you can continue to use the previous objective series. In this case, parfocality is
no longer guaranteed.
Tubes
The interface between the optics carrier and the
tube has remained the same, so existing tubes
fit the new M series. The new tubes
for eyepieces with field number 23,
predecessor models were only designed for
field number 21, resulting in a smaller object
field.
Eyepieces
However, the new M-series eyepieces models
have an audible and tangible click to provide
immediate feedback in case of accidental
adjustment.
are designed
while the
Leica M series Manual 17
On We Go
If your new Leica microscope has already been
assembled and commissioned by your Leica
consultant, click here to skip through the installation instructions and go directly to the Quick
Start Guide on page 46.
If, on the other hand, you are assembling the
microscope yourself, continue with the "Assembly" chapter, which begins on page 19.
Leica M series Manual 18
Assembly
Leica M series Manual 19
Base and Focusing Column
The first step is to connect the focusing column
of the M series to the corresponding base.
Tools used
Hex socket screwdriver, 3 mm ★
Assembling the column adapter
1. Securely install the column adapter on the
column using the four included screws.
Assembling the focusing drive
2. Securely screw the focusing column to the
base using the six included screws.
Leica M series Manual 20
Optics carrier
Tools used
Hex socket screwdriver, 4 mm ★
Assembling the optics carrier
1. Place the optics carrier on the focusing
column so that the screw fit into the thread
provided and the lug fits into the groove.
2. Press the optics carrier backwards to the
focusing column and screw it in place using
your other hand.
Leica M series Manual 21
Tube
All intermediate tubes that fit between the
optics carrier and the binocular tube are fitted
in the same manner.
Tools used
No tools required. ★
Preparations
1. Unscrew the positioning screw and remove
the protective cover.
Assembling the tube
2. Push the tube (for example, the inclined
binocular tube) into the dovetail ring and
rotate it slightly in both directions until the
positioning screw meshes with the guide
groove.
3. While holding the tube only slightly, carefully tighten the positioning screw. It
is automatically brought to the correct
position.
Leica M series Manual 22
Eyepieces
Tools used
No tools required. ★
Magnification range
You can extend the overall magnification range
using available 10×, 16×, 25× and 40× widefield eyepieces for persons wearing glasses.
Preparation
1. If you want to use an optional graticule,
insert it now (page 62).
2. Remove the plastic tube guard.
Inserting the eyepieces
3. Push the eyepieces into the tubes as far as
they will go and check to ensure that they
fit tightly and accurately.
4. Securely tighten the clamping screws.
Leica M series Manual 23
Objective
Tools used
No tools required. ★
Preparation
1. Remove the protective cap on the optics
carrier by turning it.
Attaching the objective
Hold the objective firmly during assem-
bly and disassembly so that it does not
fall onto the stage plate. This applies particularly to the 2× planapochromatic objective,
which is very heavy. Remove all specimens from
the stage plate first.
2. Screw the objective clockwise into the
optics carrier.
Alternative fastening options
If using the objective nosepiece, read the ★
instructions on page 25.
If using the AX carrier, read the instructions ★
on page 27.
Leica M series Manual 24
Objective Nosepiece – Assembly
Preparations
Hold the objectives firmly during
assembly and disassembly so that they
do not fall onto the stage plate.
Move the drive housing all the way upwards ★
and remove the optics carrier, if the carrier
has already been installed.
Assembly
1. Remove the transport anchor from the
objective nosepiece.
2. Rotate the moving part by 90° and attach
the objective nosepiece to the drive housing from the front (!). Screw the objective
nosepiece firmly into place.
3. Unscrew the three Phillips screws on the
objective mount of the optics carrier and
remove the intermediate ring.
4. Screw the optics carrier onto the objective
nosepiece.
5. Screw both objectives onto the objective
nosepiece. It makes no difference which
position an objective occupies.
6. Unscrew the locking screws on both sides
of the objective nosepiece.
You can now adjust the parfocality (see instructions on the next page).
Leica M series Manual 25
Objective Nosepiece – Adjusting Parfocality
The following procedure only has to be carried
out once. Afterwards, both objectives are parfocal, meaning that the specimen remains in focus
when the objective nosepiece is rotated.
This procedure must be repeated if you replace
either of the two objectives with another.
The following example assumes the combination of the 1× and 2× planapochromats. If
you are using another objective combination,
replace the 2× objective in the description with
the objective with the stronger magnification.
Preparation
Open the iris diaphragm. ★
Set the dioptric correction of the eyepieces ★
to "0".
Adjustment
1. Rotate the 2× objective into the beam path
and set it to the lowest magnification.
2. Focus on the specimen.
3. Rotate the 1× objective into the beam
path.
4. Turn the objective on the thread in both
directions until the specimen appears
sharp.
5. Toggle to the 2× objective.
6. Select the strongest magnification and
refocus until the specimen appears absolutely sharp.
7. Toggle to the 1× objective.
8. Turn the objective on the thread in both
directions until the specimen appears
absolutely sharp.
By means of zooming, check that the behavior
of the objective is parfocal. Repeat the check
with the other objective. If it is not parfocal,
repeat the procedure.
9. Tighten the locking screws.
Leica M series Manual 26
AX Carrier – Preparation
Tools
Hex socket screwdriver, 2.5 mm ★
Preparing the AX carrier
Before the AX carrier is installed, it may first
have to be prepared for the optics carrier to be
used (Leica M125, M205 C or M165 C).
1. Check the switch position.
If the switch position corresponds to the ★
optics carrier used, skip the following steps.
Otherwise, proceed as follows:
2. Unscrew the two screws that hold the
switch in position.
4. Important: push the switch all the way into
the mount while reinserting the screws.
Only this ensures that the switch is positioned correctly.
3. Pull out the switch, rotate it into the desired
position and reinsert it.
Leica M series Manual 27
AX Carrier – Assembly
Tools
Hex socket screwdriver, 4 mm ★
Philips screwdriver ★
Once the AX carrier has prepared for the corresponding optics carrier, it can be installed.
1. Move the slide of the AX carrier into mid
position.
Wrong Right
2. Install the AX carrier on the focusing
column.
3. Screw the objective to the AX carrier.
4. Unscrew the three holding screws and
remove the adapter ring on the optics
carrier.
5. Install the optics carrier on the AX carrier.
Leica M series Manual 28
Transmitted-light Base TL ST
Unpacking the base
The base is delivered with the adapter plate
installed. Make sure the devices are unpacked
on a flat, sufficiently dimensioned, and non-slip
surface.
Focusing drive and column
1. Unscrew the extension plate from the base
using the Allen key provided.
2. Attach your focusing drive column to the
bottom using the 4 Allen screws.
3. Reattach the adapter plate to its original
position using the 6 Allen screws.
Leica M series Manual 29
Transmitted-light Base TL BFDF: Before First Use
Removing the transport anchors
Before you can use the transmitted-
light base for the first time, it is absolutely necessary to remove the two transport
anchors as follows.
Anchor of mirror
Anchor of switching slide
Leica M series Manual 30
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