Leica M165 FC, M205 FA User Manual

Leica M165 FC and M205 FA

Discover entirely new worlds of research

with the new Leica fluorescence stereomicroscopes

Living up to Life

Bringing Ideas into the Light

Fluorescence microscopy techniques are critical for studying the functions within organisms in modern developmental, molecular, and cellular biology. Fluorescence microscopy gives researchers insight into a world normally hidden from sight. The structures within an organism and their dynamic processes can be specifically targeted with fluorescence dyes to render them visible at the sub cellular level, which helps researchers to better understand the molecular principles and complex relationships on which life itself is based.

Science in the fields of cellular and developmental biology has evolved beyond understanding microstructures and isolated processes to the study of their complex interrelationships within organisms. Sophisticated genetic and cellular studies of networks as complex as the nervous or vascular system bring these vital interactions to light.

Capturing every aspect of an organism over a wide magnification range, down to the tiniest details, requires a flexible microscope system that combines excellent optics with contrastrich fluorescence technology. From specimen preparation and manipulation, to screening and evaluating genetically engineered mutations, to high-resolution documentation and long-term studies of live model organisms; with the new Leica M-Series, Leica Microsystems offers a revolutionary stereomicroscope system that is equal to the demands of modern science.

FusionOptics™:

The Evolution of Resolution

FusionOptics™

» Combines the highest possible resolution with

outstanding depth of field

Largest zoom range in stereomicroscopy

» A single microscope for preparation tasks and

documentation

The smallest details

»Discover details that were previously invisible in stereomicroscopy

Leica Microsystems brings high resolution and depth of field

together

Until now, high depth of field and maximum resolution were always considered to be irreconcilable opposites. With

FusionOptics™, Leica Microsystems has succeeded in overcoming these limitations. Scientific studies conducted at the Institute of Neuroinformatics, a department of the ETH Zürich, confirm that the human vision system is capable of drawing the maximum information content from each eye individually and merging it to create a three-dimensional image. In the same way, the new Leica M205 FA uses the two beam paths for different tasks: the right channel delivers a high-resolution image at the largest possible numerical aperture, while the left channel presents an image with high depth of field. As a result, two apparently irreconcilable worlds are merged in the human brain: the observer receives an image with outstanding richness of detail and outstanding depth of field at the same time.

Vascular anatomy of a Zebrafish embryo as revealed by GFP expression driven by the Fli-1 promoter. Courtesy: Brant Weinstein, National Institutes of Health, Bethesda, MD

Zebrafish embryo expressing GFP under the control of the beta-actin promoter. Courtesy: Prof. Dr. Stephan C. F. Neuhauss, Professor for Neurosciences ETH Zurich and Institute for Brain Research at the University of Zurich

Periferic and central nervous (ventral cord) system of a drosophila embryo, salivary gland

Drosophila melanogaster. Dorsal view, Pupa; Green: Venus. Transgenic fluorescent protein in posterior compartment of each segment. Courtesy of Dr. Kuranaga, Dept. Genetics, Graduate School of Pharmaceutical Sciences, The University of Tokyo