Leica M165 FC, M205 FA User Manual

Leica M165 FC and M205 FA

Discover entirely new worlds of research
with the new Leica fluorescence stereomicroscopes

Living up to Life

Bringing Ideas into the Light

Fluorescence microscopy techniques are criti-

cal for studying the functions within organisms

in modern developmental, molecular, and cel-

lular biology. Fluorescence microscopy gives

researchers insight into a world normally hidden

from sight. The structures within an organism

and their dynamic processes can be specifically

targeted with fluorescence dyes to render them

visible at the sub cellular level, which helps

researchers to better understand the molecular

principles and complex relationships on which

life itself is based.

Science in the fields of cellular and developmen-

tal biology has evolved beyond understanding

microstructures and isolated processes to the

study of their complex interrelationships within

organisms. Sophisticated genetic and cellular

studies of networks as complex as the nervous

or vascular system bring these vital interactions

to light.

Capturing every aspect of an organism over a

wide magnification range, down to the tiniest

details, requires a flexible microscope system

that combines excellent optics with contrast-

rich fluorescence technology. From specimen

preparation and manipulation, to screening and

evaluating genetically engineered mutations, to

high-resolution documentation and long-term

studies of live model organisms; with the new

Leica M-Series, Leica Microsystems offers a

revolutionary stereomicroscope system that is

equal to the demands of modern science.

The Evolution of Resolution

FusionOptics

Combines the highest possible resolution with »

outstanding depth of field

Largest zoom range in stereomicroscopy

» A single microscope for preparation tasks and

documentation

The smallest details

» Discover details that were previously invisible

in stereomicroscopy

™

FusionOptics™:

Leica Microsystems brings high resolution and depth of field

together

Until now, high depth of field and maximum resolution were

always considered to be irreconcilable opposites. With

FusionOptics™, Leica Microsystems has succeeded in overcoming

these limitations. Scientific studies conducted at the Institute of

Neuroinformatics, a department of the ETH Zürich, confirm that

the human vision system is capable of drawing the maximum

information content from each eye individually and merging it

to create a three-dimensional image. In the same way, the new

Leica M205 FA uses the two beam paths for different tasks:

the right channel delivers a high-resolution image at the largest

possible numerical aperture, while the left channel presents an

image with high depth of field. As a result, two apparently irre-

concilable worlds are merged in the human brain: the observer

receives an image with outstanding richness of detail and out-

standing depth of field at the same time.

 Vascular anatomy of a Zebrafish embryo as revealed by GFP expression

driven by the Fli-1 promoter. Courtesy: Brant Weinstein, National Institutes
of Health, Bethesda, MD

 Zebrafish embryo expressing GFP under the control of the beta-actin

promoter. Courtesy: Prof. Dr. Stephan C. F. Neuhauss, Professor for
Neurosciences ETH Zurich and Institute for Brain Research at the
University of Zurich

 Periferic and central nervous (ventral cord) system of a drosophila

embryo, salivary gland

 Drosophila melanogaster. Dorsal view, Pupa; Green: Venus. Transgenic

fluorescent protein in posterior compartment of each segment. Courtesy
of Dr. Kuranaga, Dept. Genetics, Graduate School of Pharmaceutical
Sciences, The University of Tokyo









 