Leica M165 C B User Manual
Living up to Life
User Manual
Leica M125 B
Leica M165 C B
Copyright
All rights to this documentation are held by
Leica Microsystems CMS GmbH. Reproduction
of text or illustrations (in whole or in part) by
print, photocopy, microfilm or other method
(including electronic systems) is not allowed
without express written permission from Leica
Microsystems CMS GmbH.
The instructions contained in the following
documentation reflect state-of-the-art technology. We have compiled the texts and illustrations as accurately as possible. Still, we are
always grateful for comments and suggestions
regarding potential mistakes within this documentation.
The information included in this manual may
be changed without prior notice.
Revision 1.0, published March 14, 2013 by:
Leica Microsystems Wetzlar GmbH
Ernst Leitz-Straße 17-37
D-35578 Wetzlar (Germany)
http://www.leica-microsystems.com
Responsible for contents:
Marketing CMS
Leica M series User Manual 2
Function of the Microscopes
The Leica M125 B and Leica M165C B microscopes, for which this User Manual has been
written, are designed for routine examinations
of cell and tissue cultures, liquids, and sediments. This includes examining specimens
taken from the human body for the purpose
of gaining information about physiological or
pathological conditions or inborn anomalies, or
testing for safety and compatibility for potential recipients, or for monitoring therapeutic
measures.
IVD
The above-named microscopes comply with
the Council Directive 98/79/EC concerning in
vitro diagnostics.
The manufacturer assumes no liability for damage caused by, or any
•
risks arising from, using the microscope for
other purposes than those for which they are
intended or not using them within the specifications of Leica Microsystems CMS GmbH.
In such cases, the Declaration of Conformity
shall be invalid.
These (IVD) instruments are not
•
intended for use in the patient environment defined by DIN VDE 0100-710. Nor are
they designed to be combined with medical
Leica M125 B nameplate
instruments in accordance with EN 60601-1. If a
microscope is electrically connected to a medical instrument in accordance with 60601-1, the
requirements listed in EN 60601-1-1 shall apply.
Not suitable for examining potentially infectious specimens.
be operated by trained laboratory personnel
only.
Leica M165 C B nameplate
This type of instrument may
Leica M series User Manual 3
General Notes
Use in clean rooms
The Leica M series can be used in clean rooms
without any problems.
Cleaning
★ Do not use any unsuitable cleaning agents,
chemicals or techniques for cleaning.
★ Never use chemicals to clean colored
surfaces or accessories with rubberized
parts. This could damage the surfaces,
and products could be contaminated by
abraded particles.
★ In most cases, we can provide special solu-
tions on request. Some products can be
modified, and we can offer other accessories for use in clean rooms.
★ The cleaning of glass surfaces and objec-
tives in particular should be carried out
exclusively as outlined in the brochure
"Cleaning of Microscope Optics". The information can be downloaded at:
http://www.leica-microsystems.com/prod-
ucts/
. Select your product and go to the "Down-
load" page.
★ For additional information, refer to page
56.
Servicing
★ Repairs may only be carried out by Leica
Microsystems-trained service technicians.
Only original Leica Microsystems spare
parts may be used.
Responsibilities of person in charge of
instrument
★ Ensure that the Leica stereomicroscope
is operated, maintained and repaired by
authorized and trained personnel only.
Leica M series User Manual 4
Important Safety Notes
User Manual
This User Manual describes the special functions of the individual modules of the Leica M
stereomicroscopy series and contains important instructions for their operational safety,
maintenance, and accessories.
You can combine individual system articles
with articles from external suppliers (e.g. cold
light sources, etc.). Please read the User Manual
and the safety instructions from the supplier.
Before installing, operating or using the instruments, read the user manuals listed above. In
particular, please follow all safety instructions.
To maintain the unit in its original condition
and to ensure safe operation, the user must
follow the instructions and warnings contained
in these user manuals.
We guarantee the quality of our products.
Our guarantee covers all faults in materials
and manufacture. It does not, however, cover
damage resulting from careless or improper
handling.
Leica M series User Manual 5
Symbols Used
Warning! Safety hazard!
This symbol indicates especially important information that is mandatory to
•
read and observe.
Failure to comply can cause the following:
★ Hazards to personnel
★ Functional disturbances or damaged
instruments
Warning of hazardous electrical voltage
This symbol indicates especially important information that is mandatory to
read and observe.
Failure to comply can cause the following:
★ Hazards to personnel
★ Functional disturbances or damaged
instruments
Danger due to hot surface.
This symbol warns against touching hot
surfaces, e.g. those of light bulbs.
Important information
This symbol indicates additional information or explanations that are intended
to provide clarity.
Explanatory notes
★ This symbol within the text stands for addi-
tional information and explanations.
Figures
(1) Numbers in parentheses within the
descriptions relate to the figures and the
items within those figures.
Disposal
Notes on how to dispose on the microscope, its components and expend-
ables.
China RoHS 50 year EFUP (Environmentally friendly use period)
IVD labeling
nstrument for in vitro diagnostics.
IVD
I
IVD manufacturing date,
for example 11 / 2011 for
MM/YYYY
November 2011.
Leica M series User Manual 6
Safety Instructions
Description
★ The individual modules fulfill the highest
requirements for observation and documentation of Leica stereomicroscopes of
the M series.
Intended use of instrument
★ Leica Microsystems microscopes are opti-
cal instruments for improving the visibility
of objects or specimens through magnification. Accessories such as optical accessories, stands, illumination, cameras etc.
supplement the equipment configuration.
Non-intended use
★ Using the instrument in any way contrary
to the specifications in the User Manual can
lead to bodily harm and damage to objects.
Never use microscopes for in vivo examinations or eye surgery if they are not expressly
intended for such use. Never install any
other plug or unscrew optical systems
and mechanical parts unless expressly
instructed to do so in the instructions.
The instruments and accessories described in
this User Manual have been tested for safety and
potential hazards. The responsible Leica affiliate
must be consulted whenever the instrument is
altered, modified or used in conjunction with
non-Leica components that are outside of the
scope of this manual!
Unauthorized alterations to the instrument or
noncompliant use shall void all rights to any
warranty claims as well as product liability and
the Declaration of Conformity.
Place of use
★ Only use the instruments in closed, dust
free rooms and between +10 °C and
+40 °C. Protect the devices from oil, chemicals and extreme humidity. If using the
devices outdoors, protect them from dust
and moisture. Never use electrical devices
outdoors.
★ Electrical components must be placed at
least 10 cm away from the wall and away
from flammable substances.
★ Avoid large temperature fluctuations,
direct sunlight and vibrations. These conditions can distort micrographic images, for
example.
★ In warm and warm-damp climatic zones,
the individual components require special
care in order to prevent the build-up of
fungus.
Leica M series User Manual 7
Safety Instructions (continued)
Responsibilities of person in charge of
instrument
★ These Safety Instructions must be available
at the workplace.
Ensure that:
★ The M series stereomicroscopes and
accessories are operated, maintained and
repaired by authorized and trained personnel only.
★ All operators have read, understood and
observe this User Manual, and particularly
the safety regulations.
Repairs, service work
★ Repairs may only be carried out by Leica
Microsystems-trained service technicians.
★ Only original Leica Microsystems spare
parts may be used.
★ Before opening the instruments, switch off
the power and unplug the power cable.
★ Avoid contact with powered electrical
circuits, which can lead to injury.
Transport
★ Use the original packaging for shipping or
transporting the individual modules of the
Leica M stereomicroscopy series and the
accessory components.
★ In order to prevent damage from vibrations,
disassemble all moving parts that (according to the user manual) can be assembled
and disassembled by the customer and
pack them separately.
Integration in third-party products
★ When installing Leica products into third-
party products, the manufacturer of the
complete system or its dealer is responsible
for following all applicable safety instructions, laws and guidelines.
Disposal
★ Once the product has reached the end of its
service life, please contact Leica Service or
Sales about disposal.
★ Please observe and comply with the
national and federal laws and regulations
that are equivalent to EC directives such as
WEEE.
Like all electronic devices, the microscope, its accessory components and
consumables must never be disposed of with
general household waste.
Leica M series User Manual 8
Safety Instructions (continued)
Legal regulations
★ Observe the generally applicable statu-
tory and country-specific regulations for
accident prevention and environmental
protection.
EC Declaration of Conformity
★ Electrically operated accessories are
constructed based on the state of the art
of technology and are provided with an EC
Declaration of Conformity. See page 55
Health risks
Workplaces with stereomicroscopes facilitate
and improve the viewing task, but they also
impose high demands on the eyes and holding
muscles of the user. Depending on the duration of uninterrupted work, asthenopia and
musculoskeletal problems may occur. For this
reason, appropriate measures for reduction of
the workload must be taken:
Optimal arrangement of workplace, work
assignments and work flow (changing tasks
frequently). Thorough training of the personnel, giving consideration to ergonomic and
organizational aspects.
The ergonomic design and construction of the
Leica M stereomicroscopy series are intended to
reduce the exertion of the user to a minimum.
Direct contact with eyepieces can be a potential
transmission path for bacterial and viral infections of the eye.
The risk can be kept to a minimum by using
personal eyepieces for each individual or
detachable eyecups.
Careful handling
★ Exercise particular care when setting up
the instruments. If it is specified that two or
more people are required for setup, compliance with this is mandatory.
★ Never spill any liquids on electrical instru-
ments. This could cause the stereomicroscope and other equipment to become
electrically live and damage people and
instruments.
★ Never clean instruments using corro-
sive cleaning agents or those containing
acetone. For detailed information about
care, refer to the User Manual for the instrument.
★ Check the power cables regularly. Defective
power cables can cause injuries.
★ Wait for bulbs to cool off before changing
them. Touching hot bulbs can cause burns.
Leica M series User Manual 9
Safety Instructions (continued)
Light sources: safety regulations
★ Light sources pose a potential risk from
glare. Therefore, lamps have to be operated
in closed housings and in installed condition.
★ Never look directly into the beam path
(blinding hazard).
★ Do not select a white, strongly reflective
background for the specimen.
External power supply for the focusing
column
(Leica M165 C B)
Permitted power supply:
SINPRO SPU46-110
Specifications:
Input: 100-240 V AC
47-63 Hz
1.2 A
Output: 33 V DC
1.51 A
For indoor use only.
External power supply for TL5000 Ergo
Permitted power supply:
SINPRO SPU130-110
Specifications:
Input: 100-240 V AC
47-63 Hz
3.2 A
Output: 33 V DC
3.93 A
max. 130 W
For indoor use only.
Use only the power supply specified
above. Other power supplies must not
•
be used. If the original power supply fails or
is damaged, it must be replaced. Repair is not
permitted. Original power supplies are available from your Leica branch office or Leica
dealer.
Leica M series User Manual 10
Contents
Copyright 2
Function of the Microscopes 3
General Notes 4
Important Safety Notes 5
Symbols Used 6
Safety Instructions 7
The Leica M Series
Congratulations! 14
A Step Towards Infinity 15
The Electronics: Comfort, Convenience and Safety for your Experiments
16
Maximum Compatibility 17
On We Go 18
Assembly
Base and Focusing Column 20
Optics carrier 21
Tube 22
Eyepieces 23
Objective 24
TL5000 Ergo transmitted light base 25
Wiring: Connections (Leica M165 C B) 26
Cables: Cable Duct 27
Quick Start Guide
The Fastest Route to Success 29
Overview of an M Series Microscope 30
The correct interpupillary distance 31
Using the Eyepieces 32
Focusing 33
Adjusting the Resistance of the Focus Drive 34
Changing the Magnification (Zoom) 35
Click Stops and Magnifications 36
Parfocality: More Comfort and Convenience for Your Work 37
Iris Diaphragm 38
Eyepieces
Magnification Factor of the Eyepieces 40
Health Notes 41
Dioptric Correction 42
Dioptric Correction and Parfocality 43
Graticules 44
Objectives and
Leica M series User Manual 11
Optical Accessories
The Different Types of Objectives 46
Bases
Leica TL5000 Ergo Transmitted Light Base: Controls 48
Leica TL5000 Ergo Transmitted Light Base: Operation 49
Technical Data
Leica TL5000 Ergo Transmitted Light Base 52
Appendix
Calculating the Total Magnification and Field of View Diameter 54
Care, Maintenance, Contact Persons 55
Leica M series User Manual 12
The Leica M Series
Leica M series User Manual 13
Congratulations!
Congratulations on obtaining your new Leica M series stereomicroscope.
We are convinced it will exceed your expectations, as never before have
we applied our decades of experience in the areas of optics, mechanical
engineering and ergonomics in such an uncompromising manner.
The Leica M series embodies all the qualities you associate with the name
Leica Microsystems: excellent objectives, high-quality engineering, and
reliability. Furthermore, the modular design ensures that the M series
adapts perfectly to your needs—no matter which accessories you require
for your tasks.
Though the reliability and robustness of Leica stereomicroscopes is
legendary, like any high-tech product, the Leica M series requires a certain
degree of care and attention. Therefore, we recommend that you read
this manual. It contains all the information you need regarding operation,
safety and maintenance. Simply observing a few guidelines will ensure
that even after years of intensive use, your stereomicroscope will continue
to work as smoothly and reliably as on the very first day.
We wish you the best of success in your work— after all, you are now
equipped with the best tool!
Leica M series User Manual 14
A Step Towards Infinity
Ever since their introduction by Horatio S. Greenough, stereomicroscopes have worked according to the optical principles based primarily
on Ernst Abbe's research. For over a century,
ingenious optics designers and engineers have
worked to push magnification, resolution and
image fidelity to the limit permitted by optics.
In doing so, they have always been constrained
by the interrelation between three factors: the
higher a microscope's resolution, the lower the
available working distance. If one increases the
distance of the optical axes, the three-dimensional image seen by the observer becomes
distorted— a sphere becomes an ellipse, a flat
surface curves towards the observer.
Leica M series User Manual 15
The Electronics: Comfort, Convenience and Safety for your Experiments
Never before have electronics been used as
extensively in a Leica series as in the new M
series lineup. Optics carrier, tripod, base and
illuminator are all connected using electrical
contacts—which provides a number of advantages.
Contacts not only transmit data, but also supply
the power.
Fewer cables
A large part of the cables have been routed in
the interior of the column. Data are also transferred via the interface between the column,
the optics carrier and the carrier. The direct
result is that you need fewer cables—this not
only makes your workstation neater and more
comfortable, it even makes it appear larger.
Leica M series User Manual 16
Maximum Compatibility
Leica engineers were careful to ensure that the
new Leica M series—like its predecessors—
remains compatible with existing series. This
means that objectives, bases, tubes and so on
can be reused.
Objectives
All new objectives of the Leica M series are
parfocal.
If you prefer, you can continue to use the previous Leica objective series. In this case, parfocality is no longer guaranteed.
Tubes
The interface between the optics carrier and
the tube has remained the same, so existing tubes fit the new M series. The new tubes
are designed for eyepieces with field number
23, while the predecessor models were only
designed for field number 21, resulting in a
smaller object field.
Eyepieces
The new M series eyepieces have an audible
and tangible click to provide immediate feedback in case of accidental adjustment.
Leica M series User Manual 17
On We Go
If your new Leica microscope has already
been assembled and commissioned by your
Leica consultant, click here to skip through
the installation instructions and go directly
to the Quick Start Guide on page 28.
If, on the other hand, you are assembling the
microscope yourself, continue with the "Assembly" chapter, which begins on page 19.
Leica M series User Manual 18
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