Leica DFC425 C, DFC425 User Manual

Leica DFC425 / DFC425 C

Digital Microscope cameras for Analysis and Documentation

Living up to Life

Fast and Easy Analysis and Documentation

Stunning, High-Resolution Details

Excellent picture quality is essential for precise image analysis, documentation, and
reporting. The Leica DFC425 and DFC425 C Digital Microscope Cameras provide de­tailed high-resolution pictures with outstanding accuracy and brilliant color reproduc­tion. The exceptional picture quality and ease of use, makes these cameras the perfect
choice for all analysis and documentation needs.

Excellent Picture Quality

These cameras digitize the image information from the CCD chip directly in the camera
head, which leads to excellent noise suppression and perfect acquisition of the un­processed CCD signal. Digitization takes place with a resolution of 12 bits and Leica’s
true color calibration takes care of the natural color reproduction, which produces
excellent picture quality.

DFC425 / DFC425 C Highlights

Live image control provides fast

•

focusing and positioning of the
sample.

Quickly transfer images with

•

standard FireWire 1394b inter­face for PCs.

XGA (1024

• ×768) progressive

scan preview with up to 20
frames per second

5-megapixel CCD Bayer Array

•

RGB filter produces brilliant
pictures.

Exposure times range from

•

1 msec to 60 sec (DFC425 C: to
600 sec).

Features 36-bit RGB color depth

•

Partial scan mode offers the fas- •
test scanning of a freely defined
area at full resolution

Easily and quickly connects to

•

all microscopes via a c-mount
interface

An intuitive user interface offers

•

convenient image capture and
processing functions

Two-color LED displays operati-

•

onal status
Ultra compact housing saves

•

space.

DFC425 C Highlights

Peltier cooling for high dynamic •
range and minimum noise under
low light

2

• ×2 binning mode for increased

brightness and faster frame rate
in low light situations

Compact Design

The camera’s compact housing, specifi cally desi­gned for microscopy applications, is easy to at­tach to the microscope. The camera is not much
larger than a computer mouse and does not re­quire an external power supply, which reduces
workstation clutter.

Easy to Use

Leica’s digital technology simplifi es all operations,
from image capture through image archiving, and
allows digital retouching and analysis. In addition,
intelligent camera options allow you to conveni­ently set up the camera parameters. Leica came­ras have automatic white balance and advanced
illumination control and are thus ready to produce
perfect images in seconds.

3

3

COOLED

Leica M125 with Leica DFC425 C Digital Microscope Camera, LED Illumination,
Trinocular Tube, SmartTouch™ and PC System with Leica LAS Software

DFC425 C for Low-Light Applications

The DFC425 C allows crisp, sharp ima­ges to be created without noise, even
under low illumination. Interfering ther­mal noise is effectively reduced with
active cooling by means of a Peltier ele­ment. With the innovative, fast readout
procedure, even high-resolution low light
recording is now a reality.

High-performance Leica LAS software

The Leica Application Suite (LAS) software included in the scope of
supply offers numerous functions for recording and retouching ima­ges. Beginners as well as experienced users can thus use the full po­tential of the digital technology. The captured images can be edited,
analysed, archived and reproduced as often as you wish without any
loss in quality. The TWAIN driver included in the delivery can be used
to transfer photographs to other image editing programs without any
problems.

Leica DFC425 / DFC425 C – Technical Data

Digital Camera Leica DFC425 / DFC425 C

Camera type Digital camera for microscopy with control software

Sensor Interline transfer frame readout CCD – ICX452

Sensor Grade/Size Grade Zero / 8.10 mm × 6.64 mm, Diagonal 8.93 mm (Type 1/1.8”)
Color Filter RGB Bayer Mosaic

Protective color Filter Hoya CM500 S (IR cut off 650 nm), removable

Shutter control
Number of pixels / pixel size 5 Megapixel, 2592 ×1944 / 2.78 µm × 2.78 µm
Color depth 36 Bit
A/D converter 12 Bit
Dynamic range DFC425: > 57 dB / > 700:1 dB DFC425 C: > 58 dB / > 800:1 dB

Exposure time DFC425: 1 msec – 60 sec DFC425 C: 1msec – 600 sec

Readout noise σ < 6 LSB (12 Bit) typical
Gain control/Offset control 10× / 0.. 255 LSB (12 Bit)
Shading correction Yes, stored for all formats
Cooling DFC425: not available DFC425 C: Δ –20° compared to ambient
Region of interest Freely adjustable in 2 pixels steps from 2 × 2 up to full resolution

Image Formats Pixels Speed f.p.s., Fast / HQ

Interlaced large 2592 × 1944 6/3
Interlaced medium 1728 × 1296 9/4.5
Progressive large 1024 × 768 18/ 9
Progressive medium 864 × 648 20/ 10
Progressive small 576 × 432 40/ 20

Modes Formats in Fast (40MHz) or High Quality (20MHz) modes

Computer Hardware Software

Min. computer configuration Pentium 4, 2.5 GHz, 1 GB RAM Leica DFC Twain

24 bit graphics, 1024 × 768, Leica LAS Software

6-pin or 9-pin FireWire OHCI Windows Vista 32/64 bit

or free PCI slot Windows XP prof. 32/64 bit, SP3
Interfaces
Recommended video adapter c-mount 0.5× or 0.55×

Data Single cable FireWire – IEEE1394b – 9 pin, screw lock

Digital Input connector Opto-decoupled trigger
Digital Output connector Flash synch or readout active

Physical and Environmental

Power consumption DFC425: ~4 W DFC425 C: ~6 W
Power supply via FireWire cable

Housing Aluminum die cast

Size DFC425: 112 × 74 × 69 mm³
Weight DFC425: 340 g

Operating temperature range +5 to +35 °C

Relative humidity 10 % .. 80 % non-condensing
Equipment Order Numbers
12 730 221 Leica DFC425 camera kit comprising: Leica DFC425 Camera, Leica software, FireWire cable a–b
12 730 222 Leica DFC425 C camera kit comprising: Leica DFC425 C Camera, Leica software, FireWire cable a–b

Additional Items

12 730 210 PCI-Express FireWire-b card for PCs without FireWire
12 447 066 Laptop PCMCIA FireWire-a interface card

12 730 186 FireWire-cable, 3m, b–b, 9/9-Pin
12 730 187 FireWire-cable, 3m, a–b, 6/9 Pin
12 730 188 FireWire Power kit comprising: 110/220V power supply, 4-pin FireWire-a or 6-pin FireWire-a adapter

Electronic global shutter / 3 frames interlaced readout

DFC425 C: 132 × 74 × 69 mm³
DFC425 C: 495 g

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10IDC15010EN • © Leica M icrosy stems (S witzer land) Lt d • CH-9 435 Hee rbrugg, 2 009 • P rinted i n Switz erland – IX .20 09 – RDV – Il lustra tions, d escript ions and tec hnical dat a are not bin ding and may b e changed wi thout no tice.