Leica DFC345 FX User Manual

Leica DFC345 FX

High Resolution, High Sensitivity Monochrome Camera
for Fluorescence Applications

Detailed Documentation of

Fluorescence Specimens at High Speed

Low light fl uorescent documentation creates special demands
for digital cameras. To address these applications, Leica Micro­systems has completed the portfolio of powerful digital cameras
with the new high-resolution, high-sensitivity Leica DFC345 FX. It
is ideally suited for detailed documentation of applications where
only limited light is available.

Brilliant and detailed fl uorescence imaging

The 2-megapixel CCD sensor with a pixel size of 4.4 µm ensures
that each image reveals extraordinary details. High resolution
combined with the high dynamic range offered by the Leica
DFC345 FX allows dark (non-labeled) and bright (fl uorescent­ labeled) specimens to be monitored within the same image with
the fi nest documentation of details.

Cultured cortical neurons of the rat (DAPI, Cy2, Cy5),
FAN GmbH, Magdeburg. Z-stack acquired with
Leica AF6000.

Under diffi cult conditions with minimum light intensity, photo­electric elements must be cooled to produce noise-free images.
Leica’s method of active cooling via a Peltier element effectively
reduces background noise in the image. The combination of low
background and high quantum effi ciency makes this camera ide­ally suited for high contrast documentation of even weak fl uores­cence signals.

The automated research microscope Leica DM6000 B

with the digital camera Leica DFC345 FX.

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