Leica DFC310 FX
High Sensitive Digital Color Fluorescence Camera
Fast Acquisition of F
The digital color camera Leica DFC310 FX is designed for demanding applications in cell biology, developmental biology, and
medicine. Stained histological sections as well as fl uorescence
labelled specimens can be documented with highest color fi delity. Active cooling of sensor elements using a Peltier element
creates noise-free images even at the lowest light intensities.
Moreover, various acquisition modes such as binning allow for the
precise monitoring of fast dynamic processes in living cells: the
new Leica DFC310 FX is ideally suited for GFP-expressing or vital
stained micro and macro specimens.
Cross section of Drosophila eye, triple fl uorescence
Tadpole endothelium, Differential Interference Contrast
Colorful documentation
Due to its 36 bit color depth and the option of automated white
balance, stained specimen or macro specimen can be documented with highest color fi delity. The 1392 x 1040 pixel of the Leica
DFC310 FX ensure that even the faintest detail is captured.
GFP-expressing embryos of zebrafi sh.
Courtesy of: Dr. Brant Weinstein, National Institutes
of Health, Bethesda, MD
2
High quality fl uorescence images
At the heart of the Leica DFC310 FX is a 2/3” progressive scan interline
sensor. The broad dynamic range is a prerequisite for the simultaneous capture of dark (non-labeled) and bright (fl uorescent labeled)
areas in the specimen. Even faint labeled probes can be displayed
due to the high sensitivity of the new Leica DFC310 FX. The cooling of
the chip effectively reduces the noise level of the signal.
Capture motion with precision
In Partial Scan Mode, freely defi nable areas can be captured with
precision. The extremely high scanning frequency allows effi cient
documentation of 3D moving objects at full camera resolution. The
camera delivers up to 71 images per second in 4 x 4 binning mode.
The top scan rate allows fast focusing using the monitor and perfect
parameter adjustment. At the same time, specimen photobleaching is avoided. Recording can also be performed in high resolution
mode.
Intuitive imaging solutions for PC and MAC
The software that accompanies the camera ensures fast and easy
capturing of digital images. The Leica DFC310 FX is PC or MAC compatible, and the camera is easy to operate using an interface specifi cally designed for microscopy applications. Numerous intuitive
image capture and editing functions ensure immediate availability of
high quality recorded images for viewing and processing. The new
Leica DFC310 FX allows all the advantages of digital technology to
be fully utilized.
Many advantages for your research application
• 1.4 megapixel CCD with Bayer Array RGB Filter
• 36 Bit RGB color depth
• High linearity over the complete dynamic range
• Variable exposure times (4 µsec – 10 min)
• Due to active cooling of the sensor minimized
noise levels
• Binning mode ideal for fast acquisition
of living cells
• Partial Scan Mode allows fastest readout
of free defi
nable regions of interest (ROI)
• Up to 71 frames per second
• FireWire b interface for fast data transfer
• For PC and MAC environment
• Easy to use software features useful functions
for acquisition and processing
Equipment Components
Order No.
11 547 001 Leica DFC310 FX camera set
for MAC
11 547 002 Leica DFC310 FX camera set
for PC
The automated research microscope Leica DM6000 B
with the digital color camera Leica DFC310 FX
and Leica Application Suite software.
1,0
0,90
0,80
0,70
0,60
0,50
0,40
0,30
Relative Response
0,20
0,10
0,00
400 450 500 550 600 650
Quantum effi ciency of Leica DFC310 FX
(acquired with Twain, white balance applied)
Wavelength (nm)
3
Technical Data: Leica DFC310 FX
Digital Camera Leica DFC310 FX
Camera type Digital color high-sensitivity cooled camera for fl uorescence microscopy with control software
Sensor
Sensor grade/size Grade Zero/10.2 mm x 8.3 mm, diagonal 11 mm (Type 2/3)
Color fi lter RGB Bayer mosaic
Protective color fi lter Hoya CM500S (IR cut-off at 650 nm)
Shutter control Electronic global shutter/progressive scan readout
Number of pixels 1.4 megapixel, 1392 x 1040
Max. scaled resolution (PC only) 3.3 megapixel, 2088 x 1560
Sensitive area 9 mm x 6.7 mm
Pixel size 6.45 μm x 6.45 μm
Color depth 36 Bit
A/D converter 12 Bit
Dynamic range > 62 dB (typical)
Readout noise < 3 LSB (12 Bit) typical
Full well capacity 16000 electrons
Exposure time 4 μsec. – 10 min.
Dark current 0.04 e-/pixel/sec at 12 Bit typical
Quantum effi ciency (relative) Blue 470 nm 77%; Green 530 nm 73%; Red 600 nm 100%
Offset control 0 – 255 LSB (12 Bit)
Live image On computer screen
Shading correction Yes, stored for all formats
Brightness correction On all binning modes
Cooling Active Peltier thermoelectric cooling element
Cooling temperature
Region of interest (ROI) Freely adjustable in 1 pixel steps from 1 x 1 up to full resolution
Image Formats Pixels Speed f.p.s.
Full frame fast 1392 x 1040 20
Full frame HQ 1392 x 1040 10
Binning 2 x 2 fast 696 x 520 39
Binning 2 x 2 HQ 696 x 520 19
Binning 4 x 4 fast 348 x 260 71
Binning 4 x 4 HQ 348 x 260 35
Modes Formats in fast (40
Computer PC MAC
Min. computer confi guration
Supported operating systems
Software Leica DFC Twain (6.12) Leica Firecam
Interfaces
Optical C-Mount
Recommended video adapter 0.7x
Data Single cable FireWire – IEEE1394b 9-pin
Digital input connector Opto-decoupled trigger
Digital output connector Flash Sync or readout active
Software trigger Async trigger
Physical and Environmental Data
Power consumtion ~5 W
Power supply
Housing Aluminium die cast
Size 132 x 74 x 69 mm
W
eight 503 g
Operating temperature range +5 – +35°C
Relative humidity 10% to 80% non-condensing
Interline progressive scan CCD – ICX285 EXview HAD CCD sensor
Δ – 20°K to ambient
MHz) or high quality (20 MHz) modes as indicated above, trigger or free running
Pentium 4, 2 GHz, 512 MB RAM
24-Bit grahics, 1024 x 768
CD-ROM drive
9-Pin FireWire-OHCI or free PCI slot
Windows XP
Via FireWire cable
, Vista (32 and 64 bit) MAC OS 10.4 and 10.5
G4 or G5,
512 MB RAM
CD-ROM drive
Order nos. of the editions in: English 914665 • German 914 666 • Part-No. 501-378 • VIII/10/DX/••.H. • LEICA and the Leica Logo are registered trademarks of Leica IR GmbH.
www.leica-microsystems.com
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