Leica CM3600 XP User Manual
Instructions for use
Leica CM3600 XP, 6v3, English - 01/2013
Order-No.: 14 0417 80101 RevB
Always keep this manual with the instrument.
Read carefully before working with the instrument.
Leica CM3600 XP
Cryomacrotome
NOTE
The information, numerical data, notes and value
judgments contained in this manual represent the
current state of scientific knowledge and stateof-the-art technology as we understand it following thorough investigation in this field. We are
under no obligation to update the present manual
periodically and on an ongoing basis according
to the latest technical developments, nor to provide our customers with additional copies, updates etc. of this manual.
To the extent permitted in accordance with the
national legal system as applicable in each individual case, we shall not be held liable for erroneous statements, drawings, technical illustrations etc. contained in this manual. In particular,
no liability whatsoever is accepted for any financial loss or consequential damage caused by or
related to compliance with statements or other
information in this manual.
Statements, drawings, illustrations and other
information as regards contents or technical
details of the present manual are not to be
considered as warranted characteristics of our
products. These are determined only by the contract provisions agreed between ourselves and
our customers.
Leica reserves the right to change technical
specifications as well as manufacturing processes without prior notice. Only in this way is it
possible to continuously improve the technology
and manufacturing techniques used in our products.
This document is protected under copyright laws.
All copyrights to this documentation are held by
Leica Biosystems Nussloch GmbH.
Any reproduction of text and illustrations (or of
any parts thereof) by means of print, photocopy,
microfiche, web cam or other methods – including any electronic systems and media – requires
express prior permission in writing by Leica
Biosystems Nussloch GmbH.
For the instrument serial number and year of
manufacture, please refer to the name plate at
the back of the instrument.
© Leica Biosystems Nussloch GmbH
Published by:
Leica Biosystems Nussloch GmbH
Heidelberger Str. 17 - 19
D-69226 Nussloch
Germany
Phone: +49 62 24 143-0
Fax: +49 62 24 143-2 68
Internet: http://www.LeicaBiosystems.com
Leica CM3600 XP – Cryomacrotome
3
Table of contents
1. Important Information ........................................................................................................ 8
1.1 Symbols in the text and their meanings .......................................................................................................................... 8
1.2 Qualification of personnel .................................................................................................................................................. 8
1.3 Instrument type..................................................................................................................................................................... 8
1.4 Designated use ..................................................................................................................................................................... 9
1.5 Warranty .............................................................................................................................................................................. 10
1.6 Service information ........................................................................................................................................................... 10
1.7 Decommissioning and disposal ...................................................................................................................................... 10
2. Characteristics of the Leica CM3600 XP ...................................................................... 12
2.1 Layout of the CM3600 XP .................................................................................................................................................. 12
2.2 Technical Data ................................................................................................................................................................... 14
2.2.1 Microtome type ...................................................................................................................................................... 14
2.2.2 Control unit ......................................................................................................................................................... 14
2.2.3 Dimensions and weight ........................................................................................................................................ 15
2.2.4 Electrical connections.......................................................................................................................................... 15
2.2.5 Refrigeration system - cooling chamber .......................................................................................................... 16
2.2.6 General parameters .............................................................................................................................................. 17
2.3 Instrument parts ................................................................................................................................................................. 17
2.4 Function................................................................................................................................................................................ 18
3. Safety................................................................................................................................... 20
3.1 Safety notes ........................................................................................................................................................................ 20
3.2 Warnings.............................................................................................................................................................................. 20
3.3 Safety devices .................................................................................................................................................................... 24
3.3.1 Emergency stop switch ........................................................................................................................................ 24
3.3.2 Knee lever ......................................................................................................................................................... 25
3.3.3 Window ......................................................................................................................................................... 25
3.3.4 Cryochamber ......................................................................................................................................................... 25
4. Site requirements .............................................................................................................26
4.1 Site requirements at place of installation ..................................................................................................................... 26
4.2 Electrical connections ...................................................................................................................................................... 27
4.3 Other connections ............................................................................................................................................................. 27
5. Installation ......................................................................................................................... 28
5.1 Unpacking and installation .............................................................................................................................................. 28
5.2 Standard delivery ............................................................................................................................................................... 28
5.3 Port and switch panel ....................................................................................................................................................... 29
5.3.1 Port and switch functions .................................................................................................................................... 30
5.3.2 Fuses ......................................................................................................................................................... 32
5.4 PC .......................................................................................................................................................................................... 33
4
Instructions for use, 6v3, RevB – 01/2013
Table of contents
6. Software ............................................................................................................................. 34
6.1 Start and log-in procedure............................................................................................................................................... 34
6.2 Description of the window elements ............................................................................................................................. 35
6.2.1 Title bar ......................................................................................................................................................... 35
6.2.2 Tool bar ......................................................................................................................................................... 35
6.2.3 Work space ......................................................................................................................................................... 37
6.2.4 Information bar ....................................................................................................................................................... 37
6.3 Initialization ......................................................................................................................................................................... 38
6.4 Main window ...................................................................................................................................................................... 40
6.4.1 Chamber temperature .......................................................................................................................................... 41
6.4.2 Time ......................................................................................................................................................... 41
6.4.3 Automatic defrost cycle ....................................................................................................................................... 41
6.4.4 Automatic dehydration ......................................................................................................................................... 42
6.4.5 Knife movement ..................................................................................................................................................... 42
6.4.6 Extraction system .................................................................................................................................................. 45
6.4.7 Sectioning program .............................................................................................................................................. 45
6.4.8 Sledge speed ......................................................................................................................................................... 46
6.4.9 Mode of operation ................................................................................................................................................. 47
6.4.10 Cutting window ...................................................................................................................................................... 48
6.5 Parameter settings ............................................................................................................................................................ 49
6.5.1 Parameter setting .................................................................................................................................................. 50
6.5.2 Password list ......................................................................................................................................................... 54
6.5.3 Configuration ......................................................................................................................................................... 57
6.5.4 Reference voltages ............................................................................................................................................... 64
6.5.5 Language selection ............................................................................................................................................... 64
6.5.6 Initialization ......................................................................................................................................................... 64
6.6 Temperature curves .......................................................................................................................................................... 65
6.6.1 Work space ......................................................................................................................................................... 66
6.6.2 Diagram ......................................................................................................................................................... 68
6.7 Chronological event list .................................................................................................................................................... 69
6.7.1 Work space ......................................................................................................................................................... 70
6.7.2 Event table ......................................................................................................................................................... 72
6.8 Alarm list .............................................................................................................................................................................. 73
6.8.1 Work space ......................................................................................................................................................... 74
6.8.2 Event table ......................................................................................................................................................... 75
6.8.3 Error codes: meaning and troubleshooting ..................................................................................................... 76
6.9 Chamber illumination ........................................................................................................................................................ 77
6.10 Section documentation ..................................................................................................................................................... 77
6.10.1 Tree diagram ......................................................................................................................................................... 78
6.10.2 Studies protocol ..................................................................................................................................................... 79
6.10.3 Event table ......................................................................................................................................................... 82
6.11 Photo mode ......................................................................................................................................................................... 83
6.12 Screen printout ................................................................................................................................................................... 83
6.13 Remote inquiry .................................................................................................................................................................... 84
6.14 Help ....................................................................................................................................................................................... 86
Leica CM3600 XP – Cryomacrotome
5
Table of contents
6.15 Saving ................................................................................................................................................................................... 86
6.16 Printing labels ..................................................................................................................................................................... 86
6.17 Foot switch .......................................................................................................................................................................... 86
6.18 User log-in / log-out ........................................................................................................................................................... 87
6.19 Quitting the application ..................................................................................................................................................... 87
7. Operating the Leica CM3600 XP ..................................................................................... 88
7.1 Check list.............................................................................................................................................................................. 88
7.2 Switching the instrument on ............................................................................................................................................ 89
7.3 Starting the software......................................................................................................................................................... 89
7.4 Setting the chamber temperature .................................................................................................................................. 89
7.5 Specimen stages ................................................................................................................................................................ 90
7.5.1 Inserting the specimen stage, orientable, with ball-joint ............................................................................. 92
7.5.2 Inserting the specimen stage, non-orientable ................................................................................................ 94
7.6 Knife ...................................................................................................................................................................................... 96
7.6.1 Inserting standard knives .................................................................................................................................... 98
7.6.2 Inserting the disposable blade holder with disposable blade ................................................................... 100
7.6.3 Adjusting the knife angle ................................................................................................................................... 102
7.7 Setting the trimming parameters .................................................................................................................................. 103
7.8 Setting the sectioning parameters ............................................................................................................................... 104
7.8.1 Sectioning the specimen ................................................................................................................................... 104
7.8.2 Collecting the sections ....................................................................................................................................... 104
7.9 Dehydrating the sections ............................................................................................................................................... 106
8. Cleaning and Maintenance .......................................................................................... 108
8.1 Cleaning ............................................................................................................................................................................. 108
8.1.1 Removing the microtome from the cryochamber ......................................................................................... 108
8.2 Maintenance ..................................................................................................................................................................... 110
8.2.1 Oiling 110
8.2.2 Defrosting 111
9. Optional accessories ..................................................................................................... 112
9.1 Ordering information ....................................................................................................................................................... 112
6
Instructions for use, 6v3, RevB – 01/2013
Leica CM3600 XP – Cryomacrotome
7
1. Important Information
1.1 Symbols in the text and their meanings
Warnings
appear in a gray box and are marked
by a warning triangle.
Useful tips,
i.e. important information for the user,
appear in gray boxes and are marked
by an information symbol.
ENTER
(5)
Buttons and function keys which need
to be pushed on the data entry screen,
are printed in bold capitals.
Numbers in parentheses refer to item
numbers in illustrations.
1.2 Qualification of personnel
1.3 Instrument type
All information contained in this Instructions for
use applies exclusively to the instrument type
indicated on the title page.
Caution!
This Instructions for use is valid ONLY
for the instrument with Prod. No.
0163/08.2010
Refer to the nameplate attached to
the rear side of the instrument.
Only trained laboratory personnel may operate the
Leica CM3600 XP.
All laboratory personnel designated to operate the
Leica instrument must read this Instructions for
use carefully and must be familiar with all technical features of the instrument before attempting
to operate it.
8
Instructions for use, 6v3, RevB – 01/2013
1.4 Designated use
1. Important Information
The Leica CM3600 XP is a PLC-controlled and motorized precision
cryomacrotome for large-surface sections, equipped with a convectioncooled cryochamber for deep-freezing, sectioning, storing and freezedrying large biological and industrial specimens.
The instrument is designed for the above described applications only and
must be operated according to the instructions in the present Instructions
for use.
Any use of the instrument not described in this Instructions for
use is considered improper.
If you wish to use the instrument for special applications or modes of
operation not listed in this manual, it is absolutely necessary that you
contact your local Leica representation prior to doing so.
Otherwise, you will loose the Leica warranty on your instrument.
Only trained personnel, skilled in the use of the above-mentioned applications, may operate the Leica CM3600 XP.
Prior to operating the instrument, the operator must thoroughly read and
understand this Instructions for use.
Leica CM3600 XP – Cryomacrotome
9
1. Important Information
1.5 Warranty
Leica Biosystems Nussloch GmbH guarantees that the contractual product
delivered has been subjected to a comprehensive quality control procedure based on the Leica in-house testing standards, and that the product is
faultless and complies with all technical specifications and/or agreed characteristics warranted.
The scope of the warranty is based on the content of the concluded agreement. The warranty terms of your Leica sales organization or the organization from which you have purchased the contractual product shall apply
exclusively.
1.6 Service information
If you require technical service or replacement parts, please contact your
Leica sales representative or Leica dealer who sold the product.
Please provide the following information:
• Model name and serial number of the instrument
• Location of the instrument and name of the person to contact
• Reason for the service call
• Date of delivery
1.7 Decommissioning and disposal
The instrument or parts of the instrument must be disposed of according to
existing applicable, local regulations.
10
Instructions for use, 6v3, RevB – 01/2013
1. Important Information
Leica CM3600 XP – Cryomacrotome
11
2. Characteristics of the Leica CM3600 XP
2.1 Layout of the CM3600 XP
PC
Port and
switch panel
Refrigeration
and control unit
Microtome
(in chamber)
Window Chamber lidEmergency stop switch
Drain valve for
condensation
water
Cryochamber
Knee lever
12
Instructions for use, 6v3, RevB – 01/2013
2. Characteristics of the Leica CM3600 XP
Drain valve
Condensation water is drained through the drain valve. The tray at the
drain valve must remain at the instrument at all times and must be emptied regularly.
Refrigeration and control unit
The refrigeration and control unit cools the cryochamber and controls the
microtome and the entire electronics.
Knee lever
The knee lever is used for manual operation of the specimen sledge.
PC
Commercial PC, monitor, keyboard, mouse and internal modem.
Emergency stop switch
Press the emergency stop switch to stop the microtome immediately.
Cryochamber cabinet
The cryochamber cabinet contains the cryochamber which houses the
microtome.
Chamber lid
The chamber lid can be opened completely for maintenance and cleaning
work on microtome and/or cryochamber.
Window
The window is heated and can be opened completely.
Microtome
The microtome is used to section the specimens.
Port and switch panel
The port and switch panel contains the mains switch and several sockets
and ports.
Leica CM3600 XP – Cryomacrotome
13
2. Characteristics of the Leica CM3600 XP
2.2 Technical Data
2.2.1 Microtome type
450 C Sliding microtome
Section thickness selection: 1 - 200 μm
Sectioning range: 0 - 450 mm
Max. sectioning speed: 80mm/s
Knife retraction after sectioning stroke: 50 - 250μm
Total vertical stroke of knife: 70mm (standard)
Total vertical stroke of knife: 100mm (version with
Total horizontal stroke: 450mm
Specimen orientation via ball-joint: ca. 5° (x/y/z axis)
Specimen orientation w/large spec. stage
Maximum specimen size (L x W x H): 450 x 150 x 70mm
Maximum specimen size (L x W x H): 450 x 150 x 200mm
Knife holder for steel and
tungsten carbide knives: 160mm
Knife holder for disposable blade holder: 160 mm
Knife (160 mm) with facet angle of: 20°, 35°
extended vertical stroke)
(450 x 150mm)
Rotation around
longitudinal axis (x-axis)
(standard)
(version with extended
stroke)
2.2.2 Control unit
14
The Leica CM3600 XP is equipped with a commercial PC, monitor,
keyboard, mouse and an internal modem. For further information,
please refer to the manual of the PC.
Operating system: XP.
Software: CM3600 XP Version 3.2.2.
Instructions for use, 6v3, RevB – 01/2013
2.2.3 Dimensions and weight
Inner (cryochamber L x H x W): 1650 x 720 x 600mm
Outer (L x H x W): 2800 x 1090 x 920mm
Total dimensions with connecting lines:
Outer length (total): 2800 mm
Outer length (chamber only): 1940 mm
Outer length (control unit only): 860 mm
Outer height (total): 1090 mm
Outer height (work surface height): 1050 mm
Outer width: 920 mm
Weight (total, incl. microtome): 660 kg
Weight (microtome only): 190 kg
2.2.4 Electrical connections
380 V / 50 Hz, three-phase
Nominal voltage: 380V AC ± 10 %
Nominal frequency: 50 Hz
Line voltage tolerance: ± 10 %
Power draw: 2400VA
Circuit breaker/ 3 x 8A / 1 x 10A
Power safety switch to DIN IEC 127-2
2. Characteristics of the Leica CM3600 XP
208 V / 60 Hz, two-phase
Nominal voltage: 208 V AC
Nominal frequency: 60 Hz
Line voltage tolerance: ± 10 %
Power draw: 2400VA
Automatic cutout/ 2 x 8A / 1 x 16A
Power safety switch UL-listed
200 V / 50 Hz, two-phase
Nominal voltage: 200 V AC
Nominal frequency: 50 Hz
Line voltage tolerance: ± 10 %
Power draw: 2400VA
Automatic cutout/ 2 x 8A / 1 x 16A
Power safety switch UL-listed
Leica CM3600 XP – Cryomacrotome
15
2. Characteristics of the Leica CM3600 XP
200 V / 60 Hz, two-phase
Nominal voltage: 200 V AC
Nominal frequency: 60 Hz
Line voltage tolerance: ± 10 %
Power draw: 2400 VA
Automatic cutout/ 2 x 8A / 1 x 16A
Power safety switch UL-listed
220 V / 60 Hz, two-phase
Nominal voltage: 220 V AC
Nominal frequency: 60 Hz
Line voltage tolerance: ± 10 %
Power draw: 2400 VA
Automatic cutout/ 2 x 8A / 1 x 16A
Power safety switch UL-listed
2.2.5 Refrigeration system - cooling chamber
16
Selectable temperature range: 0° C to -30 °C
at an ambient temperature
of 22°C, closed window
and humidity ≤ 60%: -30°C ±10 % (adjustable
in 1 K increments)
Control accuracy: +1 to -3 K
Electrical power draw: 1160 W
Refrigerating output at -25°C: 1370 W
Nominal pressure: 20bar (maximum value)
Cutoff pressure: 25bar (via Pressostat)
Safety factor: 2, as per UL 1262
Refrigerant: 1100g, refrigerant R404A
Compressor oil: 1220 ccm alpha 22, Kyodo
Defrost: Automatic hot gas defrost, programmable,
2 x automatic hot gas defrost / 24 hours.
Duration: 1 - 15 minutes, manual defrosting as needed.
Instructions for use, 6v3, RevB – 01/2013
2.2.6 General parameters
Noise information
2.3 Instrument parts
2. Characteristics of the Leica CM3600 XP
Protective class: I
Pollution degree: 2
Overvoltage installation category: II
A-weighted noise level: 78.5 dB (A)
Distance between sound meter and instrument: 1 meter
Measuring uncertainty: ±1.5 dB (A)
Instrument settings at measurement: Normal instrument
operation with
extraction
The user must wear hearing protection when using the
extraction.
The Leica CM3600 XP consists of a sliding microtome in a large-volume
cryochamber. The instrument is designed for cryosectioning large specimens or for processing specimens with the so-called cryo-planing
technique.
The large-scale heavy-duty microtome is made for sectioning large
biomedical and industrial specimens such as whole animals or large
tissue specimens by applying the sectioning techniques for large-surface
sections. The sturdy construction of the instrument also permits sectioning very hard specimens such as undecalcified bones.
The microtome is housed in a convection-cooled large-volume
cryochamber. The large chamber in connection with the special cooling
system enables dehydration of sections within a very short time.
The low temperatures are achieved and maintained by cold air circulating inside the cryochamber. A fan controls the air circulation speed.
The large specimens are frozen onto metal specimen stages which are
subsequently clamped in the specimen sledge.
Optionally the instrument can be operated with an extraction system
which largely prevents the cryochamber being soiled with section waste.
Leica CM3600 XP – Cryomacrotome
17
2. Characteristics of the Leica CM3600 XP
2.4 Function
The motor-driven specimen sledge moves horizontally underneath the
knife, either producing a section (cryosectioning) with each stroke or a
high-quality specimen surface (cryo-planing technique).
Prior to each sectioning stroke, a stepper motor feeds the microtome
knife downwards towards the specimen by a selected amount of microns
(section thickness).
During each return stroke of the specimen sledge, the stepper motor
automatically carries out a knife retraction movement, programmable
between 50 and 250 μm. This protects both the knife edge and the specimen surface from being damaged.
Before starting the next sectioning stroke, the motor lowers the knife
towards the specimen surface by the selected sectioning feed plus the
set value for knife retraction.
Trimming is programmable; the desired section thickness can be
preselected.
The number of sections to be performed during trimming can be programmed via the section counter. When the programmed number of
sections has been carried out, the microtome will stop automatically.
Once the desired specimen block height has been reached, those sections that will actually be used for further examination are collected
manually via the knee lever.
The Leica CM3600 XP is operated via PC and/or manually via the knee
lever. All data entries are made via the PC.
The Leica CM3600 XP features two automatic defrost cycles.
Defrost cycle duration is software-controlled. If the evaporator surface is
already completely deiced before the programmed defrost time is up, the
defrost cycle will be aborted. In addition, manual defrost cycles can be
set as needed.
18
Instructions for use, 6v3, RevB – 01/2013
2. Characteristics of the Leica CM3600 XP
Leica CM3600 XP – Cryomacrotome
19
3. Safety
The safety and caution notes in this chapter must be observed at all times.
Be sure to read these notes even if you are already familiar with the operation and use of
other Leica products.
3.1 Safety notes
This Instructions for use contains important instructions and information regarding the operational safety and maintenance of the instrument.
The Instructions for use is an important part of
the product, and must be read carefully prior to
startup and use and must always be kept near
the instrument.
If additional requirements on accident prevention and environmental
protection exceeding the scope of
this Instructions for use are imposed
by laws/regulations of the country
of operation, this Instructions for
use must be supplemented by appropriate instructions to ensure
compliance with such
requirements.
The protective devices located on the instrument and the accessories must not be
removed or modified. Only qualified service personnel authorized by Leica may repair
the instrument and access its internal components.
This instrument has been built and tested in
accordance with the safety requirements for
electrical equipment for measurement, control,
and laboratory use.
In order to maintain this condition and ensure safe
operation, the operator must observe all the
instructions and warnings contained in this
Instructions for use.
For current information about applicable standards, please refer to the
CE declaration for the instrument on
our Internet site:
http://www.LeicaBiosystems.com
3.2 Warnings
The safety devices installed in this instrument by the manufacturer only constitute the basis for
accident prevention. Operating the instrument safely is, above all, the responsibility of the owner,
as well as the designated personnel who operate, service or repair the instrument.
To ensure trouble-free operation of the instrument, make sure to comply with the following
instructions and warnings.
20
Instructions for use, 6v3, RevB – 01/2013
3. Safety
The protective devices on the instrument must neither be
removed nor modified.
Only connect the instrument to a grounded power socket.
Do not interfere with the grounding function by using an
extension cord without a ground wire.
Use extreme caution when handling microtome knives and
disposable blades.
Handle knives and blades as per manufacturer's directions.
Never operate the instrument in rooms with explosion hazard.
Leica CM3600 XP – Cryomacrotome
During instrument setup / installation do not switch the
instrument on before being instructed to do so by this manual.
While working through the Instructions for use, do not use
either the knife or the specimen block.
The lid of the instrument must always be opened by two
persons. No one must work on the instrument until it has
been ensured that the lid has latched into place.
21
3. Safety
Below you find a summary of all warnings contained in this Instructions
for use.
Any use of the instrument not described in this Instructions for
use is considered improper.
Always swing the knee lever upwards before leaving the
instrument.
The chamber lid must be lifted by 2 persons when opened.
When opening the chamber lid, the lid retainer must engage.
Only when the lid is lifted can the user work in the instrument.
Prior to any work involving the knife, microtome or inside
the cryochamber, activate the emergency stop switch.
For further information, refer to chapter 3.3.1.
22
The chamber lid must be closed at all times when the instrument is operated, as otherwise the emergency stop switch is
not accessible.
The user must be able to reach the specimen easily while
working on the microtome. During all work, wear closefitting clothing and use safety glasses.
Caution when using the foldaway extraction!
Before you manipulate the specimen or the knife, the extraction must always be folded all the way to the side. Otherwise,
it could fold back and press your hand against the knife.
Instructions for use, 6v3, RevB – 01/2013
3. Safety
Prior to any work involving the knife, microtome or inside the
cryochamber, swing the knee lever upwards.
For any work involving the knife, microtome or inside the
cabinet always wear special protective gloves.
For reasons of safety and accuracy, the maximum trimming
thickness should not exceed 100 μm per section.
When trimming biological material (particularly bone),
section thickness should not exceed 50μm.
Never try to catch or grab the knife.
Never introduce your hands into the space between
specimen and knife.
Leica CM3600 XP – Cryomacrotome
Caution when adjusting the knife holder from
100 to 200mm – a crushing hazard exists.
Radioactive specimen waste must be disposed of according
to the local radiation safety regulations.
For removing the microtome (approx. 190kg) from the
cryochamber, a ceiling crane capable of carrying that weight
should be available.
For safety reasons, we strongly advise you against lifting the
microtome (approx. 190kg) from the cryochamber without
appropriate lifting gear. Do not attempt to lift the microtome
from the chamber manually, even if several persons are there
to help.
23
3. Safety
3.3 Safety devices
3.3.1 Emergency stop switch
The Leica CM3600 XP is equipped with an emergency stop switch (1)
located on the chamber surface to the left of the window.
Activating the emergency stop switch
Press the Emergency stop switch (1) to stop the microtome immediately.
Always press the emergency stop switch before making modifications to the microtome, knife or specimen (see warning
message below the disc) to prevent accidental activation of
the microtome using the knee lever.
1
24
Resetting the emergency stop switch.
To reset the emergency stop switch (1) turn it in the direction of the
arrow until it is released upwards back to its original position.
Instructions for use, 6v3, RevB – 01/2013
3.3.2 Knee lever
3. Safety
3.3.3 Window
3.3.4 Cryochamber
1
Never leave the instrument unattended when
the knee lever is folded out (1) to prevent the
microtome from being set in motion accidentally.
Always swing the knee lever upwards before leaving the instrument.
The window is heatable and equipped with a
lock button (2). To open the window, lift lock
button (2) slightly upwards and turn it by 180°.
2
Before opening the large chamber lid, first
close the window and lock with the lock
button (2).
When opening the chamber lid, make sure the
lid retainer (3) engages to prevent the chamber
3
lid from falling.
Leica CM3600 XP – Cryomacrotome
When opening the chamber lid,
the lid retainer must engage.
25
4. Site requirements
4.1 Site requirements at place of installation
• Relative humidity not over 60 % and non-condensing.
• Room temperature consistently between +5 °C and +22°C.
• Room volume must be at least 30 m3.
• The substrate must have sufficient load capacity and rigidity for the weight
of the instrument, 660kg.
• Do not operate the instrument in rooms with explosion hazard.
• The instrument is designed for indoor use only.
• The instrument must be connected to a
grounded socket. Only use the cable provided;
never use an extension cable.
• The power plug and emergency stop switch
must be without obstruction.
• Avoid vibrations, direct sunlight and heavy
variation in temperature.
• Installation elevation up to 2000 m above sea
level.
If possible at all, the room should be air-conditioned. If ambient conditions are not maintained
as specified, instrument performance may be
negatively affected (lowest specified temperature
may not be reached, frost may accumulate).
Approx. 3.5 m
All transport paths for the Leica CM3600 XP must
be at least 1.50 m wide; especially doorways must
have that width. This specification already takes
into account the swivel range of the instrument
when passing through doors (if it is possible to
keep the instrument straight while passing though
doors, a door width of 95cm is sufficient). The
Leica CM3600 XP has a total length (refrigeration
and control unit and cryochamber) of 2.70m or,
with the connecting lines on the left side of the
refrigeration and control unit, of approx. 2.80m,
i.e. a minimum width of the installation wall of
2.90m is required. Ideally the wall should measure 3.50 m more to provide easy access to the
left side of the unit for technical service work. The
required minimum distance between the back
panel of the instrument and the wall is 30cm, the
recommended distance is 50cm.
Installation wall, ideal dimensions (view from above)
0.5 m
26
Approx. 0.8 m
Leica CM3600 XP
Approx. 2.7 m
High room temperatures and excessive
air humidity affect
the cooling capacity
of the instrument.
Instructions for use, 6v3, RevB – 01/2013
4.2 Electrical connections
4. Site requirements
4.3 Other connections
Country: Europe USA Japan
Installation cord cross section: 2.5mm
2
2.5mm
2
2.5mm
2
Fuse/connection: 16 A/380 V 25 A/208 V 25 A/200 V
Power cable length is 3m
Leakage current > 3.5 mA
A second protective conductor terminal is located on the instrument.
Both terminals must be used.
There must not be any differences in potential between the two
protective conductor terminals.
Telephone connection
For remote failure diagnosis, a telephone connection must be available to
hook up the modem. The phone connection must be direct, i.e. must not
be controlled by an additional computer (server).
Compressed air supply
If you want to use the optional extraction system:
Compressed air supply for the extraction system (p = 5 - 8 bar) must be
available.
Leica CM3600 XP – Cryomacrotome
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5. Installation
5.1 Unpacking and installation
Only duly trained personnel may unpack and install the Leica CM3600 XP.
Please contact your local Leica sales organization for appropriate advice.
5.2 Standard delivery
The standard delivery includes:
• 1 Leica CM3600 XP, consisting of:
• Cabinet
• Refrigeration and control unit
• PC (computer, monitor, keyboard, mouse)
• Microtome 450 C with integrated knife holder
• 1 Disposable blade holder 157 AR
• 1 Dispenser with 10 disposable blades, type H 45 L
• 1 Roll of section collecting tape, type 810
• 1 Roll of section collecting tape, type 4248
• 1 Bottle of low temperature oil PDP 40, 500 ml
• 1 Positioning tool for disposable blade holder
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• 1 Tool set, consisting of:
• Allen keys, set of 7, in different sizes: 1.5, 2.0, 2.5, 3.0, 4.0, 5.0, 6.0
• Fork wrenches, set of 2, sizes 15 and 17
• 1 Syringe, 10 ml with injection needle,
• 1 Instructions for use
• 1 Set of manuals for PC
• 1 Pressure plate
• 1 Plug for alarm contact
• 1 Pair of protective gloves
Check the delivery carefully against the packing list, delivery
note and your order.
If you notice any discrepancies, please contact your local
Leica sales office or dealer immediately.
Instructions for use, 6v3, RevB – 01/2013
5.3 Port and switch panel
5. Installation
Port and switch panel
Interface
RJ 45
Connection for
foot switch
Connection for
compressed air
GFCI
switch
Main switch
Switch for
local
alarm signal
Connection for
external alarm
Control
lamps
Circuit
breaker
Caution!
Only the POWER SWITCH disconnects the instrument
from the power supply!
To do so, move the switch into the "0" position.
Leica CM3600 XP – Cryomacrotome
29
5. Installation
5.3.1 Port and switch functions
Main switch
In the "0" position, this switch disconnects the instrument from the power
supply.
Connection for footswitch
Optionally, a foot switch can be connected for the purpose of section
thickness documentation (GLP).
RS 232 interface
The RS 232 interface links the PC to the PLC.
Switch for local alarm signal
To activate the local alarm function, press the alarm signal switch.
The green LED in the switch button lights up.
To deactivate the local alarm function, press the alarm signal switch
once again. The green LED in the switch button is extinguished.
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Compressed air supply
Connection of compressed air hose to extraction unit.
Control lamps
Indicate function of the refrigerating sets.
Instructions for use, 6v3, RevB – 01/2013
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