LEICA CM1900 User Guide

Leica CM1900
Cryostat
Instruction Manual
Leica CM1900 – V5.3 English - 10/2006 Always keep this manual near the instrument!
Read carefully prior to operating the instrument!
The information, numerical data, notes and value judg­ments contained in this manual represent the current state of scientific knowledge and state-of-the-art tech­nology as we understand it following thorough investi­gation in this field. We are under no obligation to up­date the present manual periodically and on an ongo­ing basis according to the latest technical develop­ments, nor to provide our customers with additional copies, updates etc. of this manual.
For erroneous statements, drawings, technical illus­trations etc. contained in this manual we exclude li­ability as far as permissible according to the national legal system applicable in each individual case. In par­ticular, no liability whatsoever is accepted for any fi­nancial loss or consequential damage caused by or related to compliance with statements or other infor­mation in this manual.
Statements, drawings, illustrations and other informa­tion as regards contents or technical details of the present manual are not to be considered as warranted characteristics of our products. These are determined only by the contract provisions agreed between our­selves and our customers.
Leica Microsystems Nussloch GmbH Heidelberger Str. 17 - 19 D-69226 Nussloch
Leica reserves the right to change technical specifi­cations as well as manufacturing processes without prior notice. Only in this way is it possible to continu­ously improve the technology and manufacturing tech­niques used in our products.
This document is protected under copyright laws. Any copyrights of this document are retained by Leica Microsystems Nussloch GmbH.
Any reproduction of text and illustrations (or of any parts thereof) by means of print, photocopy, microfiche, web cam or other methods – including any electronic systems and media – requires express prior permis­sion in writing by Leica Microsystems Nussloch GmbH.
For the instrument serial number and year of manu­facture, please refer to the name plate at the back of the instrument.
© Leica Microsystems Nussloch GmbH
Tel.: 0 62 24 - 143 0 Fax: 0 62 24 - 143 200 eMail: histo_info@leica-microsystems.com Internet: http://www.histo-solutions.com
Leica CM1900 – Cryostat
3

2. Table of contents

2. Table of contents.................................................................................................................................................................... 4
3. Safety instructions for handling the instrument ............................................................................................................... 5
4. Technical data ........................................................................................................................................................................ 8
5. General description ............................................................................................................................................................. 10
5.1 Optional models .................................................................................................................................................................... 10
5.2 Designed use/improper handling ...................................................................................................................................... 10
5.3 Standard delivery ................................................................................................................................................................. 10
6. Unpacking the instrument .................................................................................................................................................. 11
6.1 How to open the crate ......................................................................................................................................................... 11
6.2 How to remove the packing materia ................................................................................................................................. 11
6.3 Mounting the ramp ............................................................................................................................................................... 12
6.4 Transport to the installation site ........................................................................................................................................ 13
7. Installation ............................................................................................................................................................................ 14
7.1 Site requirements ................................................................................................................................................................. 14
7.2 Transport to the desired site - Relocation ....................................................................................................................... 15
7.3 Assembly of the handwheel ............................................................................................................................................... 17
7.4 Locking the handwheel ....................................................................................................................................................... 17
7.5 Mounting the heat extractor .............................................................................................................................................. 17
7.6 Inserting the accessories ................................................................................................................................................... 18
7.7 Inserting the optional accessories ................................................................................................................................... 19
8. Operation ............................................................................................................................................................................... 20
8.1 Precooling the knife ............................................................................................................................................................. 20
8.2 Installing the knife holder base ......................................................................................................................................... 20
8.3 Inserting the knife holder .................................................................................................................................................... 21
8.4 Connection to the mains ..................................................................................................................................................... 21
8.5 Turning on the instrument .................................................................................................................................................. 21
8.6 Leica CM1900 – Overview ................................................................................................................................................... 22
8.7 Programming the desired values ...................................................................................................................................... 24
9. Daily operation ..................................................................................................................................................................... 30
9.1 Selection of the adecuate chamber temperature .......................................................................................................... 30
9.2 Specimen freezing ............................................................................................................................................................... 30
9.3 Activating / deactivating the specimen cooling ............................................................................................................. 31
9.4 Activating / deactivating the cryochamber cooling ...................................................................................................... 31
9.5 Inserting the specimen discs in the specimen head ..................................................................................................... 31
9.6 Inserting the knife in the knife holder ............................................................................................................................... 32
9.7 Moving the specimen towards or away from the knife via coarse feed ................................................................... 33
9.8 Trimming ................................................................................................................................................................................ 33
9.9 Adjustment of the anti-roll guide ....................................................................................................................................... 34
9.10 Sectioning .............................................................................................................................................................................. 34
10. Cleaning and disinfection .................................................................................................................................................. 35
10.1 Cleaning and disinfection ................................................................................................................................................... 35
10.2 Turning the instrument back on ......................................................................................................................................... 35
11. Removal of the microtome .................................................................................................................................................. 36
11.1 How to remove the microtome: ........................................................................................................................................ 36
12. Reinstallation of the microtome ........................................................................................................................................ 38
12.1 How to return the microtome to the cryochamber ........................................................................................................ 38
13. Maintenance ......................................................................................................................................................................... 40
13.1 General maintenance .......................................................................................................................................................... 40
13.2 Replacement of the lamp .................................................................................................................................................... 41
14. Troubleshooting ................................................................................................................................................................... 42
15. Temperature Selection Chart ............................................................................................................................................. 45
16. Optional accessories .......................................................................................................................................................... 46
16.1 Orienting specimen head .................................................................................................................................................... 46
16.2 Thermal block ....................................................................................................................................................................... 47
17. Ordering information ........................................................................................................................................................... 48
18. Warranty and service .......................................................................................................................................................... 50
4
Instruction manual V 5.3 – 10/2006

3. Safety instructions for handling the instrument

Unpacking and installation
To ensure an adequate cooling capacity, the instru­ment must be set up maintaining a minimum dis­tance from walls and furniture (see 'Technical data').
The instrument must be transported in an upright position or slightly tilted (max. 30°).
To ensure a safe transportation with a fork lift 3 people are required: one operating the fork lift, and the other 2 holding the instrument on either side to prevent it from sliding down.
Before connecting to the mains power, please check if the local voltage complies with the power rating specified on the name plate of the instrument (see also 'Technical data')!
Plug the instrument only to power sockets with ground!
After transporting, wait at least 4 hours before turn­ing the instrument on.
This waiting period is necesssary to allow the com­pressor oil , which may have been displaced dur­ing transport, to return into its original position.
Failure to comply with this will cause severe dam­age to the instruments!
Microtome knives
Take care when handling microtome knives and disposable blades. The cutting edge is extremely sharp and can cause severe injury!
Never leave knives and knife holders with a knife/ blade mounted lying around!
Do not place a knife on a table with the cutting edge facing upward!
Never try to catch a falling knife!
Prior to manipulating the knife and specimen, or changing the specimen or knife, always lock the handwheel and cover the cutting edge with the knife guard!
Always lock the handwheel and cover the cutting edge of the knife with the knife guard during breaks!
Avoid contact with cold parts of the instrument as this can cause frostbite!
Due to the weight of the instrument (170 kg) 4 people are required for transportation (2 people per handle).
Symbols used in this manual and their meaning
Warnings appear in a gray box and are marked by a warning triangle
Notes i.e. important user information appears in a gray box and is marked by an information symbol.
(5) (Fig.5)
Figures in brackets refer to item numbers in drawings or to the drawings themselves.
Leica CM1900 – Cryostat
5
3. Safety instructions for handling the instrument
Cleaning and disinfection - Turning the instrument back on
It is not necessary to remove the microtome for disinfecting the cryochamber, however, removal is possible, if required!
When disinfecting, please take appropriate protec­tive measures (gloves, mask, protective clothing, etc.).
When using detergents and disinfectants please comply with the safety precautions of the disinfec­tant manufacturer.
Only use acetone for cleaning the plastic anti-roll plate of the knife holders CN and CS. The glass anti­roll plate of the knife holder CE can be cleaned ei­ther with acetone or alcohol.
Dispose of waste liquid according to the waste dis­posal regulations!
Removal/Reinstallation of the microtome
Prior to removing the microtome, turn the instru­ment off with the mains switch and pull the mains plug!
Remove sliding window before removing the micro­tome!
Before removing the microtome, lock the handwheel in the lowest position. When removing the microtome, the specimen head will rapidly fall down and might injure the operator's hands!
The microtome must be entirely dry before reinstal­lation. Humidity inside will condense and freeze, causing malfunctions and damage!
All components removed from the cryostat must be carefully dried before returning them to the cryochamber!
The cooling chamber must be entirely dry when turning on the instrument (Frost formation)!
Do not use uncontrolled external heaters for drying the cryochamber. This can cause damage to the cooling system!
Do not turn the instrument on before the cryochamber is completely dry! Frost formation!
Dry all parts completely before reinserting them in the cryochamber!
The front panel and the slit cover of the microtome must be completely dry before turning on the in­strument!
When removing the microtome for cleaning or dis­infection, please keep in mind the safety instruc­tions in chapter 10 'Cleaning and Disinfection'.
6
Instruction manual V 5.3 – 10/2006
3. Safety instructions for handling the instrument
Handling samples - Defrosting
When working with possibly contaminated or in­fected material the general safety guidelines for laboratories must be applied!
Before defrosting the cryochamber remove all samples!
Before defrosting the specimen head remove all samples!
Never leave samples in the cryochamber! - The in­strument is not made for storing frozen specimen!
Maintenance
Turn the instrument off with the mains switch and pull the mains plug, before replacing the fuses!
Only use fuses of the same specification ! For the required values refer to chapter 4 'Technical Data'!
Turn the instrument off with the mains switch and pull the mains plug before replacing the lamp!
If the lamp is broken, it must be replaced by the tech­nical service, as the replacement involves a high risk of injury!
Only use lamps of the same specification! For the required type refer to chapter 4 'Technical Data'!
Leica CM1900 – Cryostat
7

4. Technical data

Type
Nominal voltage Nominal frequency Power draw Max. start-up current for 5 sec Protective class Mains fuses (MDA by Bussmann) Automatic fuse Pollution degree Overvoltage installation category Heat emission (max.)
-1 -2 -3 -4 -5 -6
230 V AC 120 V AC 230 V AC 240 V AC 100 V AC 100 V AC 50 Hz 60 Hz 60 Hz 50 Hz 60 Hz 50 Hz 1800 VA 1800 VA 1800 VA 1800 VA 1800 VA 1800 VA 25 A eff. 35 A eff. 25 A eff. 25 A eff. 30 A eff. 35 A eff. IIIIII T8A - T8A T8A - ­T10A T1 T15A T1 T10A T1 T10A T1 T15A M3 T15A M3 222222 II II II II II II 1800 J/s 1800 J/s 1800 J/s 1800 J/s 1800 J/s 1800 J/s
Refrigeration CM1900, 50 Hz CM1900, 60 Hz
Cryochamber
Temperature range 0 - -35 °C ± 3 K, at an ambient 0 - -35 °C ± 3 K, at an ambient
Refrigeration capacity 690 W 690 W Cut-off pressure 25 bar 25 bar Safety factor 3 3 Refrigerant* 275 g (± 5 g) refrigerant R 404A* 265 g (± 5 g) refrigerant R 404A* Compressor oil* 0,6 l EMKARATE RL22S, ICI* 0,6 l EMKARATE RL22S, ICI*
temperature of 22 °C temperature of 22 °C
Defrosting of cryochamber Automatic defrosting
Programmable: yes yes Defrosting intervals 1 defrost cycle/ 24 hours 1 defrost cycle/ 24 hours Defrosting period: 9 minutes 9 minutes Automatic stop of manual defrosting: at -5 °C at -5 °C
Manual defrosting
Defrosting period: 9 minutes 9 minutes Automatic stop of manual defrosting: at -5 °C at -5 °C
Quick freeze shelf
Max. temperature: - 43 °C (+ 0 K / - 2 K) - 43 °C (+ 0 K / - 2 K) Number of quick freeze stations: 10 10
Specimen cooling
Temperature range -10 - -50 °C ± 2 K, at an ambient -10 - -50 °C ± 2 K, at an ambient
Refrigeration capacity 320 W 320 W Cut-off pressure 25 bar 25 bar Safety factor 3 3
Refrigerant* 210 g (± 5 g) refrigerant R404A* 210 g (± 5 g) refrigerant R404A* Compressor oil* 0,4 l alpha 22, Kyodo* 0,4 l alpha 22, Kyodo*
Defrosting of specimen head
Automatic defrosting no no
temperature of 22 °C temperature of 22 °C
Manual defrosting
Defrosting period: 10 minutes 10 minutes Automatic stop of manual defrosting: 10 min. after reaching +20 °C 10 min. after reaching +20 °C
acc. to CECOMAF: liquid temperature 45 °C, evaporation temperature: -25°C according to IEC-1010; UL 3101
8
Instruction manual V 5.3 – 10/2006
4. Technical data
Microtome
Type Microtome encapsulated in the cryochamber Section thickness setting 0 - 60 μm, infinitely variable Specimen feed 25 mm Knife holder repositioning on the microtome base plate 30 mm Vertical stroke 59 mm Specimen retraction yes (US optional) Maximum specimen size Ø 40 mm
Cryostat
Dimensions Width (w/o handwheel) 790 mm Width (incl. handwheel) 890 mm Depth (cabinet only) 725 mm Depth (cabinet incl. mains plug) 800 mm Overall height 1200 mm Working height 1000 mm
Weight (instrument only) 168 kg Weight incl. accessories 170 kg
Set-up conditions
Distance to walls and furniture; calculated from the cabinet rear: 10 cm
right side: 20 cm
left side: 10 cm Plug the instrument only to power sockets with ground. Mains cable length: – 3,5 m Extendable: no The installation site must be free of draft and insolation; the floor must be free from vibrations.
Lamp
50 Hz-Version: Osram DULUX S 11 W/21 60 Hz-Version: Osram DULUX S 13 W/21
Conditions
ambient temperature: 8°C to 40 °C Operating temperature range: 0°C to -35 °C Temperature range during storage: + 5°C to +55°C Relative humidity: max. 60%, non-condensing Humidity during storage: < 60%
Leica CM1900 – Cryostat
*) Refrigerant and compressor oil must only be replaced by authorized service personnel!
9

5. General description

5.2 Optional models
The following models are available:
CM1900 with specimen retraction
CM1900 without specimen retraction
Please, indicate type when ordering!

5.3 Standard delivery

5.1 Designed use/improper handling

The rapid sectioning cryostat CM1900 is provided with a fully encapsulated microtome with an independent specimen cooling system. The cryostat CM1900 is appropriate for InVitro Diagnostic (IVD) applications. The instrument may only be operated within the scope of its designated use as described above and as per the instructions given in this manual. The instrument is CFC-free and is designed and manufactured according to VDE and UL regulations.
Samples must not be stored unattended in the cryostat for a long period since they could be destroyed in case of a power failure or malfunction of the instrument. The cryochamber temperature can rise considerably during defrosting. Heat-sensitive samples must there­fore be protected from exposure to elevated tempera­tures and removed prior to defrosting. The instrument may only be used for the specified ap­plication and operated in accordance with the instruc­tions given in this manual. Any other use of this instrument is considered as im­proper operation!
1 Basic instrument
1 Handwheel, assy. ..................................................................................................... 0416 18478
1 Heat extractor, stationary ....................................................................................... 0452 27918
1 Low-temperature stabilizer for heat extractor .................................................... 0452 27919
1 Set of specimen discs ............................................................................................. 0470 43550
- 4 specimen discs, 25 mm ................................................................................... 0416 19275
- 4 specimen discs, 30 mm ................................................................................... 0370 08587
1 One-piece storage shelf .......................................................................................... 0452 28594
1 Freezing shelf cover ................................................................................................. 0452 28624
1 Brush shelf with holder ........................................................................................... 0452 28799
1 Section waste tray ................................................................................................... 0452 28595
1 toolkit: ......................................................................................................................... 0436 43463
- 1brush, fine .......................................................................................................... 0183 28642
- 1 Leica-brush ....................................................................................................... 0183 30751
- 1 Allen key, no. 1.5 .............................................................................................. 0222 10050
- 1 Allen key, no. 2.5 .............................................................................................. 0222 04137
- 1 Allen key, no. 3.0 .............................................................................................. 0222 04138
- 1 Allen key, no. 4.0 .............................................................................................. 0222 04139
- 1 Allen key with spherical head, no. 4.0 ......................................................... 0222 32131
- 1 Allen key, no. 5 ................................................................................................. 0222 04140
- 1 Allen key with handle, no. 5............................................................................ 0194 04760
- 1 Allen key, no. 6 ................................................................................................. 0022 04141
- 1 Single-head wrench, no. 13 ........................................................................... 0330 33149
- 1 Single-head wrench, no. 16 ........................................................................... 0330 18595
1 Bottle of OCT-Compound, mounting med. f. cryosectioning, 125 ml ................ 0201 08926
1 Bottle of cryostat oil, type 407, 50 ml ..................................................................... 0336 06089
1 Storage mat ............................................................................................................... 0452 27913
1 Pair of safety gloves for cryosectioning, size S .................................................. 0340 40859
1 Instruction manual Leica CM1900 - G/E/F/S ......................................................... 0708 37105
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Instruction manual V 5.3 – 10/2006
Checking if the instrument has been transported correctly
Please check the TIP (N) TELL indicators immediately after arrival of the shipment. If the TIP (N) TELL Arrow Point is blue, this package has been on its side or tipped in transit. Please note on the Bill of Lading and check for damage.
The same unpacking instructions are inside a transparent sheet, attached to the transport crate at arrival of the shipment. The following unpacking instructions shall help you in case of packing or transporting the instrument once again.6.1

6.1 How to open the crate

6. Unpacking the instrument

1
1
1
3
2
2
7
1. Loosen 3 screws (1) at the top of the two sides of the crate (7).
2. Loosen 4 screws of the hinges (2) at the front and rear of the crate.
Do not loosen the screws of the hinges on the lid of the crate!
3. Loosen 1 screw (3) at the top between the two
4
4
4
4
4
4
hinges at the front and rear of the crate.
4. Loosen 3 screws (4) at the bottom on each of the four sides of the crate.
5

6.2 How to remove the packing materia

1. Remove the lid (5) and place on the ground beside the transport platform (11) with the foam parts fac-
6
6
7
ing down.
2. Remove the 4 foam parts (6).
8
11
Leica CM1900 – Cryostat
9
3. Lift the body of the crate (7) straight up.
4. Take out the cardboard box (8) containing the ac­cessories and the instruction manual at the front of the instrument.
10
5. Remove the dust cover (9) by pulling it upward.
6. Remove the foam part (10) at the rear.
11
6. Unpacking the instrument
11

6.3 Mounting the ramp

1. Upon removal of the foam part (10) 2 screws (12)
12
become visible on the base platform.
2. Fold down the hinges (2) at the rear and front.
3. Place the lid against the transport platform (11) from behind. The jogs (13) of the hinges (2) must point to the in­strument.
2
13
4. Push the lid to the left sliding the jogs (13) of the hinges under the heads of the screws (12).
12
13
11
12
13
12
Instruction manual V 5.3 – 10/2006
6. Unpacking the instrument

6.4 Transport to the installation site

The instrument must be transported in an up-
16
The lid (5) forms a ramp for the instrument so it can
right position or slightly tilted (max. 30°)!
be rolled off the transport platform.
Do not grip the cabinet at the lid (16)! Grip the cabinet only at the marked locations ( )!
Lengthwise movement
2
14
5
2
The wheels (14) must be guided over the hinges (2) at the front and rear. Caution: Risk of tilting!
1. Roll the instrument backwards over the ramp off the
platform with caution.
2. Move the instrument to the installation site on its
wheels.
3. The adjustable feet (15) can support the weight of
the instrument when tipped at a slight angle (max. 30°).
Tilt
15
Leica CM1900 – Cryostat
14
15
14
13

7. Installation

7.1 Site requirements

To ensure an adequate cooling capacity, the instrument must be set up maintaining a mini­mum distance from walls and furniture (see 'Technical data')!
The place of installation must meet the following re­quirements:
No direct sunlight.
Mains power socket at a distance no greater than appr. 3 m.
No drafts (air condition outlets etc.).
Even floor.
Vibration-free floor.
Only for indoore use.
Obstruction-free access to the handwheel.
Obstruction-free access to the ON/OFF switch.
Room temperature always approx. 22 °C.
Air humidity must not exceed 60 %.
Distance to walls and furniture, calculated from the cabinet:
- rear: 10 cm
- right side: 20 cm
- left side: 10 cm
No heat dissipating appliances around.
High room temperatures and excessive air humidity affect the cooling capacity of the cry­ostat and lead to the formation of condensa­tion water in the instrument!
14
Instruction manual V 5.3 – 10/2006
7. Installation

7.2 Transport to the desired site - Relocation

First, check if the location meets the conditions
specified in 'Site requirements'.
Transport the instrument to the desired location.
Observe the following:
The instrument must be transported in an up­right position or slightly tilted (max. 30°)!
Transport on wheels
On even floors and for short distances the instru­ment can be transported on wheels. Before trans­portation, screw the adjustable feet (1) completely down with the open-end wrench no. 16.
The instrument can be rolled lengthwise and slightly be tilted forward (see arrows). - Although the in­strument cannot be rolled sideways, it can be pushed carefully in that direction.
When tilting the instrument 2 people must counterbalance from the frontside to prevent the instrument from falling down and causing severe injury!
Tilt
Lengthwise movement
2
3
1
When transporting the instrument on wheels (2, 3) grip the cabinet only at the marked locations ( ).
The adjustable feet (15) can support the weight of the instrument when tipped at a slight angle (max. 30°). Unscrew the adjustable feet with the open-end wrench no. 16 (when subsequently transporting the instrument any further on wheels, do not forget to screw the adjustable feet down completely).
Leica CM1900 – Cryostat
15
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