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Table of contents
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LEICA CM1850 User Guide

Instruction Manual
Leica CM1850 V2.2 English – 12/2003 Always keep this manual near the instrument!
Read carefully prior to operating the instrument!
Leica CM1850
Cryostat
INFORMATION
The information, numerical data, notes and value judgments contained in this manual represent the current state of scientific knowledge and state­of-the-art technology as we understand it follow­ing thorough investigation in this field. We are under no obligation to update the present manual periodically and on an ongoing basis according to the latest technical developments, nor to pro­vide our customers with additional copies, up­dates etc. of this manual. For erroneous statements, drawings, techni­cal illustrations etc. contained in this manual we exclude liability as far as permissible ac­cording to the national legal system applicable in each individual case. In particular, no liabil­ity whatsoever is accepted for any financial loss or consequential damage caused by or related to compliance with statements or other infor­mation in this manual. Statements, drawings, illustrations and other information as regards contents or technical details of the present manual are not to be con­sidered as warranted characteristics of our products.
These are determined only by the contract pro­visions agreed between ourselves and our customers. Leica reserves the right to change technical specifications as well as manufac­turing processes without prior notice. Only in this way is it possible to continuously improve the technology and manufacturing techniques used in our products.
This document is protected under copyright laws. Any copyrights of this document are retained by Leica Microsystems Nussloch GmbH.
Any reproduction of text and illustrations (or of any parts thereof) by means of print, photocopy, microfiche, web cam or other methods – includ­ing any electronic systems and media – requires express prior permission in writing by Leica Microsystems Nussloch GmbH.
For the instrument serial number and year of manufacture, please refer to the name plate at the back of the instrument.
© Leica Microsystems Nussloch GmbH
Phone: +49 (6224) 143-0 Fax: +49 (6224) 143-200 eMail: histo_info@leica-microsystems.com Internet: http://www.histo-solutions.com
Leica CM1850 – Cryostat
3
Table of contents
1. Important Information ................................................................................................................ 6
1.1 Symbols used in this manual and their meaning ...................................................................................... 6
1.2 Qualification of personnel ............................................................................................................................. 6
1.3 Designated use ................................................................................................................................................ 6
1.4 Instrument type: ............................................................................................................................................6
2. Safety ............................................................................................................................................ 7
2.1 Safety features..............................................................................................................................................7
2.1.1 Locking the handwheel............................................................................................................................... 7
2.1.2 Knife guard .....................................................................................................................................................8
2.2 General information on instrument design and safe handling ..............................................................8
2.3 Operating conditions ...................................................................................................................................9
2.5 Cleaning and disinfection.........................................................................................................................10
2.6 Removal of the microtome ........................................................................................................................10
2.7 Maintenance ............................................................................................................................................... 11
3. Technical data .......................................................................................................................... 12
4. Unpacking and installation ....................................................................................................14
4.1 Site requirements........................................................................................................................................14
4.2 Transport to the desired location ...........................................................................................................14
4.3 Standard delivery........................................................................................................................................ 15
4.4 Handwheel assembly .................................................................................................................................16
5. Setup ........................................................................................................................................... 17
5.1 Connection to mains power ..................................................................................................................... 17
5.2 Prior to operation ........................................................................................................................................ 17
5.3 Leica CM1850 - Overview ..........................................................................................................................18
5.4 Mains switch and automatic mains fuse ..............................................................................................20
5.5 Turning the instrument on ........................................................................................................................ 20
6. Control panel operation........................................................................................................... 21
6.1 Control panel 1 ............................................................................................................................................ 21
6.2 Programming the desired values.............................................................................................................21
6.2.1 Setting the time ...........................................................................................................................................21
6.2.2 Setting the automatic defrost time (cryochamber) ............................................................................ 22
6.2.3 Selecting the cryochamber temperature ..............................................................................................22
6.2.4 Activation of the Peltier element (option).............................................................................................23
6.2.5 Manual defrosting of the quick freeze shelf ........................................................................................23
6.2.6 Manual defrosting of the cryochamber ................................................................................................ 24
6.3 Display lock ..................................................................................................................................................24
6.4 Control panel 2 - Electric coarse feed ................................................................................................... 25
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Instruction Manual V 2.2 – 12/2003
Table of contents
7. Daily use of the instrument..................................................................................................... 26
7.1 Specimen freezing ......................................................................................................................................26
7.1.1 Quick freeze shelf....................................................................................................................................... 26
7.1.2 Stationary heat extractor (accessory)* ................................................................................................ 26
7.2 Specimen discs ........................................................................................................................................... 27
7.2.1 Inserting the specimen discs in the specimen head .........................................................................27
7.2.2 Specimen orientation ................................................................................................................................27
7.3 Sectioning ....................................................................................................................................................28
7.3.1 Trimming the specimen .............................................................................................................................28
7.3.2 Section thickness setting ......................................................................................................................... 28
7.4 Temperaturew selection chart (in minus °C) ....................................................................................... 29
7.5 Defrosting ..................................................................................................................................................... 30
7.5.1 Automatic defrosting of the cryochamber............................................................................................30
7.5.2 Manual defrosting of the cryochamber ................................................................................................ 30
7.5.3 Manual defrosting of the quick freeze shelf ........................................................................................31
7.6 Terminating work ........................................................................................................................................ 31
7.6.1 Terminating daily work ..............................................................................................................................31
7.6.2 Shutdown for a longer period .................................................................................................................. 32
8. Troubleshooting ........................................................................................................................ 33
8.1 Error messages in the display ..................................................................................................................33
8.2 Temperature control button .....................................................................................................................33
8.3 Possible causes and remedies................................................................................................................34
9. Cleaning, disinfection, maintenance.................................................................................... 39
9.1 Cleaning ........................................................................................................................................................ 39
9.2 Spray disinfection with Leica Cryofect..................................................................................................40
9.3 Maintenance ............................................................................................................................................... 41
9.3.1 General maintenance ................................................................................................................................41
9.3.2 Removal of the microtome ........................................................................................................................ 42
9.3.3 Removal of the microtome cover.............................................................................................................44
9.3.4 Reinstallation of the microtome .............................................................................................................. 44
9.3.5 Replacement of the fuses.........................................................................................................................46
9.3.6 Replacement of the lamp ..........................................................................................................................46
10. Ordering information, optional accessories........................................................................ 48
10.1 Ordering information ..................................................................................................................................48
10.2 Optional accessories................................................................................................................................. 49
10.2.1 Mobile heat extractor................................................................................................................................49
10.2.2 Thermal block ..............................................................................................................................................49
11. Warranty and service ..............................................................................................................50
Leica CM1850 – Cryostat
5
1. Important Information
1.1 Symbols used in this manual and their meaning
Warnings appear in a grey box and are marked by a warning triangle .
Notes i.e. important user information ap­pears in a grey box and is marked by an information symbol.
Figures in brackets refer to item num-
(5)
(Fig.5)
bers in drawings or to the drawings themselves.

1.2 Qualification of personnel

The Leica CM1850 may only be operated by trained laboratory personnel. All laboratory personnel designated to oper­ate the instrument must carefully read the present instruction manual prior to starting work with the instrument.
1.3 Designated use
The Leica CM1850 is a powerful cryostat for routine as well as research applications in bi­ology, medicine and industry. The instrument has been designed for rapid freezing and sectioning of tissue samples. The instrument has not been designed for un­attended storage of tissue material. The instrument may only be operated within the scope of its designated use as described above and as per the instructions given in this manual. Any other use of the instrument is considered improper.

1.4 Instrument type:

All information given in this instruction manual applies only to the instrument type indicated on the title page.
A name plate, indicating the instrument serial number, is attached to the back of the instru­ment.
Fig. 1
6
Instruction Manual V 2.2 – 12/2003

2.1 Safety features

2. Safety

This instruction manual includes important instructions and information related to the operating safety and maintenance of the instrument. The instruction manual is an important part of the product. It must be read carefully before using the instrument for the first time and must always be kept with the instrument. If additional requirements, which exceed the scope of this manual, are imposed by regulations and/or laws on accident prevention and envi­ronmental protection in the country of operation, appropriate instruc­tions for compliance with such requirements must be added to this manual. Read this instruction manual carefully before attempting to use or oper­ate the instrument.
The instrument and its accessory equipment incorporate the following safety features: safety handwheel and knife guards on the knife hold­ers. The consistent use of these safety features and strict observation of the warnings and cautions in this manual, will safeguard the operator from accidents and/or personal injury to a great extent.

2.1.1 Locking the handwheel

2
1
For locking the handwheel rotate the handle until it is in the upper position. Push the locking pin (1) into the recess at the handwheel. The locking posi- tion is marked by a black dot (2). If necessary, move the handwheel slightly
Fig. 2
Fig. 3
Leica CM1850 – Cryostat
forth and back until the locking mechanism engages.
To unlock, push the locking pin (1) to the left from the recess at the handwheel.
Prior to manipulating the knife and specimen, or changing the specimen or knife, and during breaks, always lock the handwheel!
7
2. Safety

2.1.2 Knife guard

For every manipulation in the cryochamber, or upon changing a specimen when the knife or disposable blade is clamped, or dur­ing breaks, cover the cutting edge with the knife guard.
The knife holders CN and CS are equipped with knife guards; on the knife holder CE the glass plate of the anti-roll guide constitutes the knife guard (please refer to the separate instruction manual for your knife holder). Prior to manipulating the knife and specimen, or changing the specimen or knife, and during breaks, always lock the handwheel!

2.2 General information on instrument design and safe handling

This instrument has been built and tested in accordance with the follow­ing safety regulations on electrical measuring, control, regulating and laboratory devices:
• DIN EN 292,
• DIN EN 61010-1,
• EN 50082-1,
• EN 55011,
• IEC 1000-4 as well as according to the international quality standard,
• DIN ISO 9001.
In order to maintain this condition and to ensure safe operation, the opera­tor must observe the instructions and warnings contained in this in­struction manual.
8
Instruction Manual V 2.2 – 12/2003

2.3 Operating conditions Transport and installation

• After transporting do not turn the instrument on for a minimum of 4
• Do not operate in rooms with explosion hazard!
• To ensure an adequate cooling capacity, the instrument must be set
Connection to mains
• Before connecting to the mains power, please check if the local volt-
• During the compressor start-up the nominal voltage must not drop
• After transporting, wait at least 4 hours before turning the instrument
Defrosting
2. Safety
hours!
up with at least 10 cm distance from walls and furniture!
age complies with the power rating specified on the name plate of the instrument!
below the values specified in the ‘Technical data‘! The compressor requires a start-up current between 45 and 50 A. Therefore, the electric circuit at the place of installation must be in­spected by an electrical engineer to ensure that it meets the require­ments for a smooth operation of the instrument. A constant adequate power supply to the instrument must be en­sured at all times. Failure to comply with the above will cause severe damage to the instrument.
on. This waiting period is necessary to allow the compressor oil, which may have been displaced during transport, to return to its original position. Failure to comply with this can cause severe damage to the instru­ment.
• The quick freeze shelf may become hot during defrosting! Therefore, do not touch it!
2.4 Operating the instrument
• Take care when handling microtome knives and disposable blades. The cutting edge is extremely sharp and can cause severe injury!
Leica CM1850 – Cryostat
9
2. Safety
• Never leave knives and knife holders with a knife/blade mounted lying around!
• Do not place a knife on a table with the cutting edge facing upward!
• Never try to catch a falling knife!
• Always clamp the specimen before the knife!
• Prior to manipulating the knife and specimen, or changing the speci­men or knife, and during breaks, always lock the handwheel and cover the cutting edge with the knife guard!
• Avoid contact with cold parts of the instrument as this can cause frostbite!
• To make sure that the condensation water stemming from the de­frost cycles drains into the waste container and to avoid the risk of possible contaminations, ensure that the tap of the waste container (2, Fig. 31.1) is open when operating the instrument. Only shut the tap when draining the waste container!

2.5 Cleaning and disinfection

• It is not necessary to remove the microtome for routinely disinfecting the cryochamber. The instrument is appropriate for spray disinfection with Leica Cryofect!
• Do not use organic solvents or any other aggressive substances for cleaning and disinfection! Only use the cleaning agents and disinfectants specified in this in­struction manual such as Leica Cryofect (alcohol or common disin­fectants based on alcohol)!

2.6 Removal of the microtome

• Prior to removing the microtome, turn the instrument off with the mains switch and pull the mains plug.
• Prior to removing the microtome, bring the specimen head to the lower position with the handwheel. Otherwise the specimen head would rapidly fall down and might in­jure the operator‘s hands, when taking out the microtome.
• Wear appropriate protective gloves to take the cold microtome out of the cryochamber.
10
Instruction Manual V 2.2 – 12/2003

.2.7 Maintenance

Replacement of the fuses
2. Safety
Extended skin contact with cold parts of the instrument may cause frost bite!
• The microtome must be entirely dry before reinstallation. Humidity
inside will condense and freeze in the cold cryostat and thus cause malfunctions or damage.
• Do not use external heaters for drying the cryochamber. This can
cause damage to the cooling system!
• All components removed from the cryostat must be carefully dried
before they are replaced in the cryochamber
• Turn the instrument off with the automatic mains fuse and pull the
mains plug, before replacing the fuses.
• Only use fuses of the same specification! For the required values,
please refer to Chapter 3 ‘Technical data‘. The use of fuses other than specified by the manufacturer may cause
severe damage to the instrument!
Replacement of the lamp
Leica CM1850 – Cryostat
• Turn the instrument off with the automatic mains fuse and pull the
mains plug, before replacing the lamp.
• If the lamp is broken, it must be replaced by the technical service, as
the replacement involves a high risk of injury.
11

3. Technical data

Operating temperature range (ambient temperature): 18 °C to 40 °C. All specifications related to temperature are valid only up to an ambient temperature of 22 °C and an air humidity lower than 60%!
Type CM1850 CM1850 CM1850-08 CM1850-01
Mark of conformity CUL - - VDE Nominal voltage (±10%) 120 V AC 220 V AC 230 V AC 240 V AC Nominal frequency 60 Hz 60 Hz 50 Hz 50 Hz Power draw 1600 VA 1600 VA 1600 VA 1600 VA Max. start-up current for 5 sec 45A eff. 45A eff. 45A eff. 45A eff. Protective class I I I I Automatic mains fuse T12A T1 T12A T1 T10A T1 T10A T1 Pollution degree 2 2 2 2
Overvoltage installation category
Heat emission (max.) 1600 J/s 1600 J/s 1600 J/s 1600 J/s
Refrigeration system 50 Hz 60 Hz
Cryochamber Temperature setting range 0°C to -35°C (+ 2 K / - 0 K) 0°C to -35°C (+ 2 K / - 0 K) Defrosting automatic hot gas defrosting, automatic hot gas defrosting,
Refrigeration capacity 690 W 690 W Safety factor 3 3 Refrigerant 300 g ±5g refrigerant R 404A * 300 g ±5g refrigerant R 404A * Compressor oil 0.6 l EMKARATE RL-22S, ICI * 0.6 l EMKARATE RL-22S, ICI *
II II II II
temperature controlled temperature controlled 1 automatic defrost cycle/24 hours, 1 automatic defrost cycle/24 hours, temperature controlled temperature controlled manual defrost cycle manual defrost cycle
Quick freeze shelf Max. temperature -40°C (+ 0 K / - 2 K) - 40°C (+ 0 K / - 2 K) Number of quick freeze stations 10 10 Defrosting manual hot gas defrosting manual hot gas defrosting
time controlled time controlled Peltier element (option) Max. temperature -60°C (+5 K) -60°C (+5 K) Number of freezing stations 2 2* Defrosting together with quick freeze shelf together with quick freeze shelf
12
Instruction Manual V 2.2 – 12/2003
3. Technical data
* Refrigerant and compressor oil must only be replaced by qualified, authorized service per­sonnel!
Microtome
Rotary microtome Section thickness setting 1 - 60 µm Specimen feed 25 mm Vertical stroke 59 mm Maximum specimen size 55 x 55 mm Specimen orientation 8° (x-, y-, z-Achse) Electric coarse feed
slow 0.2 mm/s rapid 0.7 mm/s
Cryocabinet
Dimensions
Width (w/o handwheel) 600 mm Width (with handwheel) 730 mm Depth 730 mm Height 1140 mm
Weight (incl. microtome, without specimen cooling) approx.135 kg
Fluorescent lamp
50 Hz version: Osram Dulux S 11 W/21
Color: LUMILUX light white
60 Hz version: Osram Dulux S 13 W/21
Color: LUMILUX light white
➀ according to IEC-1010, UL 3101 ➁ according to CECOMAF
Liquid temperature 45°C Evaporation temperature -25°C
Please refer to section 5.2 ‘Site require­ments‘!
All CM 1850 types require the following fuses:
F1: T0,25 A Fa. Schurter, Typ FST; 6,3x32 mm or T0,25 A Fa. Littlefuse, Typ 313; 6,3x32 mm F2: T0,6 A Fa. Schurter, Typ FST; 6,3x32 mm or T0,6 A Fa. Littlefuse, Typ 313; 6,3x32 mm F3: T1,6 A Fa. Schurter, Typ FST; 6,3x32 mm or T1,6 A Fa. Littlefuse, Typ 313; 6,3x32 mm F4: T6,25 A Fa. Schurter, Typ FST; 6,3x32 mm or T6,25 A Fa. Littlefuse, Typ 313; 6,3x32 mm F5: T4 A Fa. Schurter, Typ FST; 6,3x32 mm or T4 Fa. Littlefuse, Typ 313; 6,3x32 mm
Leica CM1850 – Cryostat
13

4. Unpacking and installation

4.1 Site requirements

Do not operate in rooms with explosion hazard!To ensure an ad­equate cooling capacity, the instrument must be set up with at least 10 cm distance from walls and furniture.
• The place of installation must meet the following requirements: – no direct sunlight,
– mains power socket at a distance no greater than approxi-
mately 1.5 m, – no drafts (air condition outlets, etc.) directly over the instrument, – even floor, – mainly vibration-free floor, – obstruction-free access to the handwheel, – room temperature max. 35°C, – Air humidity must not exceed 60% High room temperatures and ex-
cessive air humidity affect the cooling capacity of the cryostat.
High room temperatures and excessive air humidity affect the cooling capacity of the cryostat.

4.2 Transport to the desired location

• Move the instrument to the installation site on its wheels (14). Please note the areas which are reinforced for tarnsporting and grip the
Tilt
cabinet only at these locations (Fig. 4).
• The adjustable feet (15) can support the weight of the instrument when tipping at a slight angle (max. 30 °).
• At the installation site unscrew the screws in the adjustable feet (15) using a fork wrench. This is necessary to ensure stability.
• Align the adjustable feet to level the instrument.
The instrument must be transported in an upright position only. When the instrument is tilted, the compressor oil is displaced. Do not grip the cabinet at the lid. Grip the cabinet only at the
marked locations ( ). The alignment of the adjustable feet is necessary to ensure an unobstructed drain of the quick freeze shelf defrosting water.
Fig. 4
16
Lengthwise movement
Sideways movement
15
14
14
Instruction Manual V 2.2 – 12/2003
4. Unpacking and installation

4.3 Standard delivery

It gives following variations: - CM1850 with Retraction ............................................. 0471 31150
- CM1850 without Retraction (only US) ..................... 0471 31148
1 Handwheel, assy. ......................................................................................... 0416 18478
1 Heat extractor, stationary ......................................................................................... 0471 30792
1 Low temperature stabilizer for heat extractor, (Parking station) ................................... 0471 30793
1 Optional clamping lever for object head ............................................................................. 0112 28806
3 Specimen discs, 25mm diameter ......................................................................................... 0416 19275
1 Section waste tray ......................................................................................... 0471 30787
1 Storage shelf, right ......................................................................................... 0471 30789
1 Storage shelf, left ......................................................................................... 0471 30790
1 Brush, fine ......................................................................................... 0183 28642
1 Leica brush ......................................................................................... 0183 30751
1 Brush shelf ........................................................................................ (0398 13088
1 Tool set:
- 1 Allen key, size 2.5 ......................................................................................... 0222 04137
- 1 Allen key, size 3 ........................................................................................ 0222 04138
- 1 Allen key with spherical head, size 4 ............................................................................. 0222 32131
- 1 Allen key, size 6 ......................................................................................... 0222 04141
- 1 Single-head wrench, size 16 ......................................................................................... 0330 18595
1 Bottle of cryostat oil, type 407, 50 ml ..................................................................................... 0336 06098
1 Bottle of OCT-Compound, mounting medium for cryosectioning, 125 ml ..................... 0201 08926
1 Instruction manual Leica CM1850 - G/E/F/S ........................................................................ 0708 37106
Leica CM1850 – Cryostat
Compare the delivered components with the parts list and your order. Should you find any discrepancies, please contact your Leica sales of­fice without delay.
A choice of different knife holders is available for the CM 1850. The knife holder is accompanied by its own separate instruction manual. Please contact your Leica sales office if the instruction manual is missing.
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