Fisher Phero User manual
Manual
PHERO-sub E series
BIOTEC-FISCHER GmbH
Daimlerstrasse 6
35447 Reiskirchen / Germany
Fon: +49-6408-6072
Fax: +49-6408-64165
e-mail: info@biotec-fischer.de
Revision: 1.0
Instruction Manual
PHEROsub E Series
Horizontal Agarose Gel
Electrophoresis Systems
Catalogue Numbers
111100-001
111100-002
111100-003
PHERO-sub 0710-E 111210-001 PHERO-sub 1008-E
111300-001
111300-002
111300-003
PHERO-sub 1010-E 111400-001
111400-002
111400-003
111400-004
PHERO-sub 1515-E
111500-001
111500-002
111500-003
PHERO-sub 2020-E 111600-001
111600-002
111600-003
111600-004
PHERO-sub 2632-E
1
Manual
PHERO-sub E series
BIOTEC-FISCHER GmbH
Daimlerstrasse 6
35447 Reiskirchen / Germany
Fon: +49-6408-6072
Fax: +49-6408-64165
e-mail: info@biotec-fischer.de
Revision: 1.0
Contents
SAFETY PRECAUTION................................................................................................. 3
PACKING LISTS:........................................................................................................... 4
PHERO-sub 0710-E.................................................................................................. 4
PHERO-sub 1010-E.................................................................................................. 4
PHERO-sub 1515-E.................................................................................................. 5
PHERO-sub 2020-E.................................................................................................. 5
PHERO-sub 2632-E.................................................................................................. 6
PHERO-sub 1008-E.................................................................................................. 6
Usage Guidance and restrictions:.................................................................................. 7
Care and Maintenance:-................................................................................................ 8
Setting up the Horizontal Gel Tanks:-............................................................................ 9
Gel Preparation:-.......................................................................................................... 10
Gel Pouring:-................................................................................................................ 11
Using trays with Casting Dams (Note not PHERO-sub 2632-E):-............................11
Using The Flexicaster (Note not PHERO-sub 2632-E) :-......................................... 11
Using Traditional tape method:-............................................................................... 12
Casting in the Mini Fast Unit:-.................................................................................. 13
Running the Gel:-......................................................................................................... 14
Gel Staining and Viewing:-........................................................................................... 15
References................................................................................................................... 15
Combs – MC Denotes Multi Channel Pipette compatible............................................ 17
Warranty...................................................................................................................... 20
2
Manual
PHERO-sub E series
BIOTEC-FISCHER GmbH
Daimlerstrasse 6
35447 Reiskirchen / Germany
Fon: +49-6408-6072
Fax: +49-6408-64165
e-mail: info@biotec-fischer.de
Revision: 1.0
SAFETY PRECAUTION
WHEN USED CORRECTLY, THESE UNITS POSE NO HEALTH RISK.
HOWEVER, THESE UNITS CAN DELIVER DANGEROUS LEVELS OF
ELECTRICITY AND ARE TO BE OPERATED ONLY BY QUALIFIED PERSONNEL
FOLLOWING THE GUIDELINES LAID OUT IN THIS INSTRUCTION MANUAL.
ANYONE INTENDING TO USE THIS EQUIPMENT SHOULD READ THE COMPLETE
MANUAL THOROUGHLY.
THE UNIT MUST NEVER BE USED WITHOUT THE SAFETY LID CORRECTLY IN
POSITION.
THE UNIT SHOULD NOT BE USED IF THERE IS ANY SIGN OF DAMAGE TO THE
EXTERNAL TANK OR LID.
THESE UNITS COMPLY WITH THE STATUTORY CE SAFETY DIRECTIVES:
73/23/EEC: LOW VOLTAGE DIRECTIVE: IEC 1010-1:1990 plus AMENDMENT
1:1992
EN 61010-1:1993/BS EN 61010-1:1993
3
Manual
PHERO-sub E series
BIOTEC-FISCHER GmbH
Daimlerstrasse 6
35447 Reiskirchen / Germany
Fon: +49-6408-6072
Fax: +49-6408-64165
e-mail: info@biotec-fischer.de
Revision: 1.0
PACKING LISTS:
PHERO-sub 0710-E
111100-001, 111100-002, 111100-003
Units include tank, lid and electrodes and include the following accessories:-
Tray Tray Dams Combs Loading Guides Cables
111100-001
111130-001
7 x 7cm (W x L)
111140-002
Pack of 2
111121-004
1mm, 8 sample
111140-003 – Strips
111140-004 – Platform
111110-006
111100-002
111131-001
7 x 10cm (W x L)
111140-002
Pack of 2
2 x 111121-004
1mm, 8 sample
111140-003 – Strips
111130-004 – Platform
111110-006
111100-003
111130-001
111131-001
111140-002
Pack of 2
2 x 111121-004
1mm, 8 sample
111140-003 – Strips
111140-004 – Platform
111110-006
PHERO-sub 1010-E
111130-001, 111130-002, 111130-003
Units include tank, lid and electrodes and include the following accessories:-
Tray Tray Dams Combs Loading Guides Cables
111130-001
111330-001
10 x 7cm (W x L)
111340-001
Pack of 2
2 x 111321-007
1mm, 16 sample
111340-003 – Strips
111340-004 – Platform
111310-006
111130-002
111331-001
10 x 10cm (W x L)
111340-001
Pack of 2
2 x 111321-007
1mm, 16 sample
111340-003 – Strips
111340-004 – Platform
111310-006
111130-003
111330-001
111331-001
111340-001
Pack of 2
2 x 111321-007
1mm, 16 sample
111340-003 – Strips
111340-004 – Platform
111310-006
4
Manual
PHERO-sub E series
BIOTEC-FISCHER GmbH
Daimlerstrasse 6
35447 Reiskirchen / Germany
Fon: +49-6408-6072
Fax: +49-6408-64165
e-mail: info@biotec-fischer.de
Revision: 1.0
PHERO-sub 1515-E
111400-001, 111400-002, 111400-003, 111400-004
Units include tank, lid and electrodes and include the following accessories:-
Tray Tray Dams Combs Loading Guides Cables
111400-001
111430-001
15 x 7cm
(W x L)
111440-002
Pack of 2
2 x 111421-007
1mm, 20 sample
111440-003 – Strips
111440-004 – Platform
111410-006
111400-002
111431-001
15 x 10cm
(W x L)
111440-002
Pack of 2
2 x 111421-007
1mm, 20 sample
111440-003 – Strips
111440-004 – Platform
111410-006
111400-003
111432-001
15 x 15cm
(W x L)
111440-002
Pack of 2
2 x 111421-007
1mm, 20 sample
111440-003 – Strips
111440-004 – Platform
111410-006
111400-004
111430-001
111431-001
111432-001
111440-002
Pack of 2
2 x 111421-007
1mm, 20 sample
111440-003 – Strips
111440-004 – Platform
111410-006
PHERO-sub 2020-E
111500-001, 111500-002, 111500-003
Units include tank, lid and electrodes and include the following accessories:-
Tray Tray Dams Combs Loading Guides Cables
111500-001
111530-001
20 x 10cm (W x L)
111540-002
Pack of 2
2 x 111521-006
1mm, 20 sample
111540-003 – Strips
111540-004 – Platform
111510-006
111500-002
111531-001
20 x 20cm (W x L)
111540-002
Pack of 2
2 x 111521-006
1mm, 20 sample
111540-003 – Strips
111540-004 – Platform
111510-006
111500-003
111530-001
111531-001
111540-002
Pack of 2
2 x 111521-006
1mm, 20 sample
111540-003 – Strips
111540-004 – Platform
111510-006
5
Manual
PHERO-sub E series
BIOTEC-FISCHER GmbH
Daimlerstrasse 6
35447 Reiskirchen / Germany
Fon: +49-6408-6072
Fax: +49-6408-64165
e-mail: info@biotec-fischer.de
Revision: 1.0
PHERO-sub 2632-E
111600-001, 111600-002, 111600-003, 111600-004
Units include tank, lid and electrodes and include the following accessories:-
Tray Combs Loading Guides Cables
111600-001
111630-001
26 x 16cm (W x L)
6 x 111621-001
1mm, 28 sample
111640-003 – Strips
111640-004 – Platform
111610-006
111600-002
111631-001
26 x 24cm (W x L)
6 x 111621-001
1mm, 28 sample
111640-003 – Strips
111640-004 – Platform
111610-006
111600-003
111632-001
26 x 32cm (W x L)
6 x 111621-001
1mm, 28 sample
111640-003 – Strips
111640-004 – Platform
111610-006
111600-004
111630-001
111631-001
111632-001
6 x 111621-001
1mm, 28 sample
111640-003 – Strips
111640-004 – Platform
111610-006
PHERO-sub 1008-E
Unit includes tank, lid and electrodes and the following accessories:-
Casting Gates Combs Cables
111200-001
111240-002
Pack of 2
2 x 111221-003
1.5mm, 8 sample
111210-006
The packing lists should be referred to as soon as the units are received to
ensure that all components have been included. The unit should be checked for
damage when received.
Please contact your supplier if there are any problems or missing items.
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