Fisher Phero User manual

Manual

PHERO-sub E series

BIOTEC-FISCHER GmbH
Daimlerstrasse 6
35447 Reiskirchen / Germany
Fon: [phone]
Fax: [phone]
e-mail: [e-mail]
Revision: 1.0

Instruction Manual

PHEROsub E Series

Horizontal Agarose Gel

Electrophoresis Systems

Catalogue Numbers

111100-001
111100-002
111100-003

PHERO-sub 0710-E 111210-001 PHERO-sub 1008-E

111300-001
111300-002
111300-003

PHERO-sub 1010-E 111400-001

111400-002
111400-003
111400-004

PHERO-sub 1515-E

111500-001
111500-002
111500-003

PHERO-sub 2020-E 111600-001

111600-002
111600-003
111600-004

PHERO-sub 2632-E

1

Manual

PHERO-sub E series

BIOTEC-FISCHER GmbH
Daimlerstrasse 6
35447 Reiskirchen / Germany
Fon: [phone]
Fax: [phone]
e-mail: [e-mail]
Revision: 1.0

Contents

SAFETY PRECAUTION................................................................................................. 3

PACKING LISTS:........................................................................................................... 4

PHERO-sub 0710-E.................................................................................................. 4

PHERO-sub 1010-E.................................................................................................. 4

PHERO-sub 1515-E.................................................................................................. 5

PHERO-sub 2020-E.................................................................................................. 5

PHERO-sub 2632-E.................................................................................................. 6

PHERO-sub 1008-E.................................................................................................. 6

Usage Guidance and restrictions:.................................................................................. 7

Care and Maintenance:-................................................................................................ 8

Setting up the Horizontal Gel Tanks:-............................................................................ 9

Gel Preparation:-.......................................................................................................... 10

Gel Pouring:-................................................................................................................ 11

Using trays with Casting Dams (Note not PHERO-sub 2632-E):-............................11

Using The Flexicaster (Note not PHERO-sub 2632-E) :-......................................... 11

Using Traditional tape method:-............................................................................... 12

Casting in the Mini Fast Unit:-.................................................................................. 13

Running the Gel:-......................................................................................................... 14

Gel Staining and Viewing:-........................................................................................... 15

References................................................................................................................... 15

Combs – MC Denotes Multi Channel Pipette compatible............................................ 17

Warranty...................................................................................................................... 20

2

Manual

PHERO-sub E series

BIOTEC-FISCHER GmbH
Daimlerstrasse 6
35447 Reiskirchen / Germany
Fon: [phone]
Fax: [phone]
e-mail: [e-mail]
Revision: 1.0

SAFETY PRECAUTION

WHEN USED CORRECTLY, THESE UNITS POSE NO HEALTH RISK.

HOWEVER, THESE UNITS CAN DELIVER DANGEROUS LEVELS OF

ELECTRICITY AND ARE TO BE OPERATED ONLY BY QUALIFIED PERSONNEL

FOLLOWING THE GUIDELINES LAID OUT IN THIS INSTRUCTION MANUAL.

ANYONE INTENDING TO USE THIS EQUIPMENT SHOULD READ THE COMPLETE

MANUAL THOROUGHLY.

THE UNIT MUST NEVER BE USED WITHOUT THE SAFETY LID CORRECTLY IN

POSITION.

THE UNIT SHOULD NOT BE USED IF THERE IS ANY SIGN OF DAMAGE TO THE

EXTERNAL TANK OR LID.

THESE UNITS COMPLY WITH THE STATUTORY CE SAFETY DIRECTIVES:

73/23/EEC: LOW VOLTAGE DIRECTIVE: IEC 1010-1:1990 plus AMENDMENT

1:1992

EN 61010-1:1993/BS EN 61010-1:1993

3

Manual

PHERO-sub E series

BIOTEC-FISCHER GmbH
Daimlerstrasse 6
35447 Reiskirchen / Germany
Fon: [phone]
Fax: [phone]
e-mail: [e-mail]
Revision: 1.0

PACKING LISTS:

PHERO-sub 0710-E

111100-001, 111100-002, 111100-003

Units include tank, lid and electrodes and include the following accessories:-

Tray Tray Dams Combs Loading Guides Cables

111100-001

111130-001
7 x 7cm (W x L)

111140-002
Pack of 2

111121-004
1mm, 8 sample

111140-003 – Strips
111140-004 – Platform

111110-006

111100-002

111131-001
7 x 10cm (W x L)

111140-002
Pack of 2

2 x 111121-004
1mm, 8 sample

111140-003 – Strips
111130-004 – Platform

111110-006

111100-003

111130-001
111131-001

111140-002
Pack of 2

2 x 111121-004
1mm, 8 sample

111140-003 – Strips
111140-004 – Platform

111110-006

PHERO-sub 1010-E

111130-001, 111130-002, 111130-003

Units include tank, lid and electrodes and include the following accessories:-

Tray Tray Dams Combs Loading Guides Cables

111130-001

111330-001
10 x 7cm (W x L)

111340-001
Pack of 2

2 x 111321-007
1mm, 16 sample

111340-003 – Strips
111340-004 – Platform

111310-006

111130-002

111331-001
10 x 10cm (W x L)

111340-001
Pack of 2

2 x 111321-007
1mm, 16 sample

111340-003 – Strips
111340-004 – Platform

111310-006

111130-003

111330-001
111331-001

111340-001
Pack of 2

2 x 111321-007
1mm, 16 sample

111340-003 – Strips
111340-004 – Platform

111310-006

4

Manual

PHERO-sub E series

BIOTEC-FISCHER GmbH
Daimlerstrasse 6
35447 Reiskirchen / Germany
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Fax: [phone]
e-mail: [e-mail]
Revision: 1.0

PHERO-sub 1515-E

111400-001, 111400-002, 111400-003, 111400-004

Units include tank, lid and electrodes and include the following accessories:-

Tray Tray Dams Combs Loading Guides Cables

111400-001

111430-001
15 x 7cm
(W x L)

111440-002
Pack of 2

2 x 111421-007
1mm, 20 sample

111440-003 – Strips
111440-004 – Platform

111410-006

111400-002

111431-001
15 x 10cm
(W x L)

111440-002
Pack of 2

2 x 111421-007
1mm, 20 sample

111440-003 – Strips
111440-004 – Platform

111410-006

111400-003

111432-001
15 x 15cm
(W x L)

111440-002
Pack of 2

2 x 111421-007
1mm, 20 sample

111440-003 – Strips
111440-004 – Platform

111410-006

111400-004

111430-001
111431-001
111432-001

111440-002
Pack of 2

2 x 111421-007
1mm, 20 sample

111440-003 – Strips
111440-004 – Platform

111410-006

PHERO-sub 2020-E

111500-001, 111500-002, 111500-003

Units include tank, lid and electrodes and include the following accessories:-

Tray Tray Dams Combs Loading Guides Cables

111500-001

111530-001
20 x 10cm (W x L)

111540-002
Pack of 2

2 x 111521-006
1mm, 20 sample

111540-003 – Strips
111540-004 – Platform

111510-006

111500-002

111531-001
20 x 20cm (W x L)

111540-002
Pack of 2

2 x 111521-006
1mm, 20 sample

111540-003 – Strips
111540-004 – Platform

111510-006

111500-003

111530-001
111531-001

111540-002
Pack of 2

2 x 111521-006
1mm, 20 sample

111540-003 – Strips
111540-004 – Platform

111510-006

5

Manual

PHERO-sub E series

BIOTEC-FISCHER GmbH
Daimlerstrasse 6
35447 Reiskirchen / Germany
Fon: [phone]
Fax: [phone]
e-mail: [e-mail]
Revision: 1.0

PHERO-sub 2632-E

111600-001, 111600-002, 111600-003, 111600-004

Units include tank, lid and electrodes and include the following accessories:-

Tray Combs Loading Guides Cables

111600-001

111630-001
26 x 16cm (W x L)

6 x 111621-001
1mm, 28 sample

111640-003 – Strips
111640-004 – Platform

111610-006

111600-002

111631-001
26 x 24cm (W x L)

6 x 111621-001
1mm, 28 sample

111640-003 – Strips
111640-004 – Platform

111610-006

111600-003

111632-001
26 x 32cm (W x L)

6 x 111621-001
1mm, 28 sample

111640-003 – Strips
111640-004 – Platform

111610-006

111600-004

111630-001
111631-001
111632-001

6 x 111621-001
1mm, 28 sample

111640-003 – Strips
111640-004 – Platform

111610-006

PHERO-sub 1008-E

Unit includes tank, lid and electrodes and the following accessories:-

Casting Gates Combs Cables

111200-001

111240-002
Pack of 2

2 x 111221-003

1.5mm, 8 sample

111210-006

The packing lists should be referred to as soon as the units are received to

ensure that all components have been included. The unit should be checked for

damage when received.

Please contact your supplier if there are any problems or missing items.

6

Manual

PHERO-sub E series

BIOTEC-FISCHER GmbH
Daimlerstrasse 6
35447 Reiskirchen / Germany
Fon: [phone]
Fax: [phone]
e-mail: [e-mail]
Revision: 1.0

Usage Guidance and restrictions:

• Maximum altitude 2,000m.

• Temperature range between 4°C and 65°C.

• Maximum relative humidity 80% for temperatures up to 31OC decreasing linearly to

50% relative humidity at 40OC.

• Not for outdoor Use.

This apparatus is rated POLLUTION DEGREE 2 in accordance with IEC 664.

POLLUTION DEGREE 2, states that: “Normally only non-conductive pollution occurs.

Occasionally, however, a temporary conductivity caused by condensation must be

expected”.

7

Manual

PHERO-sub E series

BIOTEC-FISCHER GmbH
Daimlerstrasse 6
35447 Reiskirchen / Germany
Fon: [phone]
Fax: [phone]
e-mail: [e-mail]
Revision: 1.0

Care and Maintenance:-

Cleaning Horizontal Units

Units are best cleaned using warm water and a mild detergent. Water at

temperatures above 600 C can cause damage to the unit and components.

The tank should be thoroughly rinsed with warm water or distilled water to prevent

build up of salts but care should be taken not to damage the enclosed electrode and

vigorous cleaning is not necessary or advised.

Air drying is preferably before use.

The units should only be cleaned with the following:-

Warm water with a mild concentration of soap or other mild detergent.

Compatible detergents include dishwashing liquid, Hexane and Aliphatic hydrocarbons

The units should not be left to in detergents for more than 30 minutes.

The units should never come into contact with the following cleaning agents,

these will cause irreversible and accumulative damage:-

Acetone, Phenol, Chloroform, Carbon tetrachloride, Methanol, Ethanol, Isopropyl

alcohol

Alkalis.

Rnase Decontamination

This can be performed using the following protocol:-

Clean the units with a mild detergent as described above.

Wash with 3% hydrogen peroxide (H2O2) for 10 minutes.

Rinsed with 0.1% DEPC- (diethyl pyrocarbonate) treated distilled water,

Caution: DEPC is a suspected carcinogen. Always take the necessary precautions

when using.

RNaseZAP™ (Ambion) can also be used. Please consult the instructions for use with

acrylic gel tanks.

8

Manual

PHERO-sub E series

BIOTEC-FISCHER GmbH
Daimlerstrasse 6
35447 Reiskirchen / Germany
Fon: [phone]
Fax: [phone]
e-mail: [e-mail]
Revision: 1.0

Setting up the Horizontal Gel Tanks:-

Instructions for fitting Electrode Cables.

1. Note the position of the lid on the unit. This shows the correct polarity and the

correct orientation of the cables, black is negative and red positive.

2. Remove the lid from the unit. Note if the lid is not removed, fitting the cables

may result in un-tightening of the gold plug and damage to the electrode.

3. Screw the cables into the tapped holes as fully as possible so that there is no

gap between the lid and the leading edge of the cable fitting.

4. Refit the lid.

Instructions for fitting Loading Guides (Not FMMS10).

These can be fitted to enhance visibility of the wells if desired. They can be

fitted to the white vinyl platform sheet or to the unit itself.

1. Seat the tray in the unit and note the position of the comb grooves. The

samples run black to red but the trays can be used frontward or backwards so

ensure that the comb grooves closest to the black electrode are marked.

2. Remove the tray.

3. Peel the back off of the loading guide and carefully apply the loading guide

directly to the gel platform.

The unit is now ready to be used.

9

Manual

PHERO-sub E series

BIOTEC-FISCHER GmbH
Daimlerstrasse 6
35447 Reiskirchen / Germany
Fon: [phone]
Fax: [phone]
e-mail: [e-mail]
Revision: 1.0

Gel Preparation:-

1. Table 1 below shows the volume of agarose solution required to make the desired

agarose gel for each unit tray size. For a standard 0.7% agarose gel, add 0.7

grammes of agarose to 100 ml of 1x TAE or TBE solution. The same 1 x solution

should be used in the tank buffer solution.

Table 1.

PHERO-sub 0710-E PHERO-sub 1010-E PHERO-sub 1515-E PHERO-sub 2020-E
Tray Gel

volume for

a 0.5mm

thick gel

Tray Gel

volume for

a 0.5mm

thick gel

Tray Gel

volume for

a 0.5mm

thick gel

Tray Gel

volume for

a 0.5mm

thick gel

7 x 7cm 25ml 10 x 7cm 35ml 15 x 7cm 52.5ml 20 x 10cm 100ml
7 x10cm 35ml 10 x 10cm 50ml 15 x 10cm 75ml 20 x 20cm 200ml

15 x 15cm 112.5ml

PHERO-sub 2632-E PHERO-sub 1008-E
Tray Gel volume for

a 0.5mm thick

gel

Gel volume for a

0.5mm thick gel

26 x 16cm 208ml 40ml
26 x 24cm 312ml
26 x 32cm 416ml

2. Add the agarose powder to a conical flask.

3. Add the appropriate amount of 1x TAE or TBE solution from the table above. To

prevent evaporation during the dissolving steps below, the conical flask should be

covered with parafilm.

4. Dissolve the agarose powder by heating the agarose either on a magnetic hot plate

with stirring bar or in a microwave oven. If using the microwave method, the

microwave should be set at around a 400 watt or medium setting and the flask swirled

every minute. The solution should be heated until all crystals are dissolved. This is

best viewed against a light background. Crystals appear as translucent crystals. These

will interfere with sample migration if not completely dissolved.

The gel must be cooled to between 50°C and 60°C degrees before pouring.

10

Manual

PHERO-sub E series

BIOTEC-FISCHER GmbH
Daimlerstrasse 6
35447 Reiskirchen / Germany
Fon: [phone]
Fax: [phone]
e-mail: [e-mail]
Revision: 1.0

Gel Pouring:-

The multiSUB range of units allow three different methods of gel casting:-

Casting Dams

Flexicaster

Traditional Tape

Using trays with Casting Dams (Note not PHERO-sub 2632-E):-

1. Fit the casting dams over each end of the tray and place onto a level surface.

The dams should be fitted so that there is no gap between the sides of the tray

and the groove in the dams. This will ensure that there is no possibility of gel

leakage.

2. Place the comb(s) in the grooves. Each tray has more than one comb grove so

that multiple combs can be used. Using multiple combs increases sample

number available per gel but decreases run length and care must be taken to

ensure that samples from the first wells do not migrate into the lanes of the

second comb wells.

3. Pour in the agarose carefully so as not to generate bubbles. Any bubbles that

do occur can be smoothed to the edge of the gel and dispersed using a pipette

tip.

4. Allow the agarose to set, ensuring that the gel remains undisturbed.

5. Carefully remove the gel casting gates and comb and transfer the gel including

tray to the main tank.

Using The Flexicaster (Note not PHERO-sub 2632-E) :-

1. Level the Flexicaster base by adjusting the feet so that the bubble is exactly

central.

2. Insert the desired length tray into the Flexicaster such that one end of the tray is

pushed up and seals against the silicone mat of the permanent end of the

Flexicaster.

3. Position the movable end of the Flexicaster so that the silicone mat is pushed

against the other end of the tray.

11

Manual

PHERO-sub E series

BIOTEC-FISCHER GmbH
Daimlerstrasse 6
35447 Reiskirchen / Germany
Fon: [phone]
Fax: [phone]
e-mail: [e-mail]
Revision: 1.0

4. Turn the cam so that the silicone mat tightly seals against the side of the tray.

Pour in the agarose carefully so as not to generate bubbles. Any bubbles that

do occur can be smoothed to the edge of the gel and dispersed using a pipette

tip.

5. Allow the agarose to set, ensuring that the gel remains undisturbed.

6. Carefully remove the gel casting gates and comb and transfer the gel including

tray to the main tank.

Using Traditional tape method:-

1. Autoclave or plastic backed general tape should be used. A length 5cm longer

than the width of each end of the tray should be cut. One length should be

placed over one end of the tray and stuck m1cm in from the tray edge. This

should then be folded and the edges sealed securely. Repeat for the other end

and place onto a level surface for gel pouring.

2. Place the comb(s) in the grooves. Each tray has more than one comb grove so

that multiple combs can be used. Using multiple combs increases sample

number available per gel but decreases run length and care must be taken to

ensure that samples from the first wells do not migrate into the lanes of the

second comb wells.

3. Pour in the agarose carefully so as not to generate bubbles. Any bubbles that

do occur can be smoothed to the edge of the gel and dispersed using a pipette

tip.

4. Allow the agarose to set, ensuring that the gel remains undisturbed.

5. Carefully remove the gel casting gates and comb and transfer the gel including

tray to the main tank.

12

Manual

PHERO-sub E series

BIOTEC-FISCHER GmbH
Daimlerstrasse 6
35447 Reiskirchen / Germany
Fon: [phone]
Fax: [phone]
e-mail: [e-mail]
Revision: 1.0

Casting in the Mini Fast Unit:-

1. Fit the casting gates into the grooves in the unit which are 10mm from the

platinum wire. Ensure that these are seated as deeply as possible. This will

ensure that a good seal is formed and that there is no possibility of gel leakage.

A small amount of Vaseline or sealing grease on the bottom and side edges of

the gates will give further leakage protection.

2. Place the comb(s) in the grooves. Each tray has more than one comb grove so

that multiple combs can be used. Using multiple combs increases sample

number available per gel but decreases run length and care must be taken to

ensure that samples from the first wells do not migrate into the lanes of the

second comb wells.

3. Pour in the agarose carefully so as not to generate bubbles. Any bubbles that

do occur can be smoothed to the edge of the gel and dispersed using a pipette

tip.

4. Allow the agarose to set, ensuring that the gel remains undisturbed.

5. Carefully remove the gel casting gates and comb.

13

Manual

PHERO-sub E series

BIOTEC-FISCHER GmbH
Daimlerstrasse 6
35447 Reiskirchen / Germany
Fon: [phone]
Fax: [phone]
e-mail: [e-mail]
Revision: 1.0

Running the Gel:-

1. Mix the sample to be loaded with sample buffer – see solutions for common sample

buffers. Usually 3ul of sample buffer is adequate but less may be used with sample

volumes of less than 10ul.

2. Fill the unit with buffer until the gel is just flooded with buffer. This will give the

fastest resolution times. For enhanced quality of resolution of sample, fill the unit to

5mm above the gel.

3. Load the samples into the wells using pipettes. Multi-channel pipettes can be used

for

loading samples with MC compatible combs, see listing in accessories for identification

of these.

4. Carefully place the lid on the tank and connect to a power supply.

5. Typically gels are run at between 90 and 150 volts. However, maximum voltages

are indicated on the serial badge of each unit. It should be noted that higher voltages

generally give faster but poorer quality sample resolution.

14

Manual

PHERO-sub E series

BIOTEC-FISCHER GmbH
Daimlerstrasse 6
35447 Reiskirchen / Germany
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Fax: [phone]
e-mail: [e-mail]
Revision: 1.0

Gel Staining and Viewing:-

The Multi Sub trays and the Mini Fast unit allow staining to be performed without

removing the gel from the tray (unit for Fast Mini) if this is preferred.

1. Transfer the gel to a vessel containing the appropriate volume of 0.5 µg/ml ethidium

bromide stain for 15–30 minutes, see solutions for stock stain concentration and adjust

to the volume used accordingly. The entire gel should be covered.

NOTE:- Ethidium bromide is a suspected carcinogen and the necessary safety

precautions should be undertaken.

2. De-stain the gel for 10–30 minutes in distilled water again ensuring the gel is

completely immersed.

3. Rinse the gel twice for a couple of seconds with distilled water.

4. Transfer the gel to a UV Transilluminator.

5. The samples will often appear as brighter, clearer bands when photographed or

viewed using a gel documentation system. However if the gel bands are too faint

then the staining procedure should be adjusted so that there is less de-staining. If

there is too much background then the staining procedure should be adjusted so

that there is more de-staining.

References

1. Sambrook, Fritsch, and Maniatis, Molecular Cloning A Laboratory Manual,

Second Edition,

Cold Spring Harbor Laboratory Press, 1989.

2. Current Protocols in Molecular Biology, Greene Publishing Associates and

Wiley-Interscience,1989.

15

Manual

PHERO-sub E series

BIOTEC-FISCHER GmbH
Daimlerstrasse 6
35447 Reiskirchen / Germany
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Fax: [phone]
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Revision: 1.0

Solutions:-

1x TAE 40 mM tris (pH 7.6), 20 mM acetic acid, 1 mM EDTA.

50x (1L) dissolve in 750 ml distilled water:
242 g tris base (FW = 121)

57.1 ml glacial acetic acid
100 ml 0.5 M EDTA (pH 8.0).
Fill to 1 litre with distilled water.

1x TBE 89 mM tris (pH 7.6), 89 mM boric acid, 2 mM EDTA
10x (1L) dissolve in 750 ml distilled water:
108 g tris base (FW = 121)
55 g boric acid (FW = 61.8)
40 ml 0.5 M EDTA (pH 8.0)
Fill to 1 litre with distilled water.

Sample Loading Dye

10x sample buffer stock consists of 50% glycerol, 0.25% bromophenol blue, and

0.25% xylene cyanole FF in 1x TAE buffer. Only 1–10 ml of the 10x loading dye
should be prepared.

Ethidium Bromide Solution

Add 10 mg of Ethidium Bromide to 1 ml distilled water.

16

Manual

PHERO-sub E series

BIOTEC-FISCHER GmbH
Daimlerstrasse 6
35447 Reiskirchen / Germany
Fon: [phone]
Fax: [phone]
e-mail: [e-mail]
Revision: 1.0

Combs – MC Denotes Multi Channel Pipette compatible.

PHERO-sub 0710-E PHERO-sub 1010-E PHERO-sub 1515-E PHERO-sub 2020-E

No. of
Samples

Part Number No. of

Samples

Part Number No. of

Samples

Part Number No. of

Samples

Part Number

0.75mm Thick Combs

1 111120-001 1 111320-001 1 111420-001 1 111520-001
2 111120-002 2 111320-002 2 111420-002 2 111520-002
4 111120-003 4 111320-003 4 111420-003 4 111520-003
8 111120-004 8 111320-004 10 111420-004 10 111520-004
8 MC 111120-005 10 MC 111320-005 10 MC 111420-005 16 111520-005
10 111120-006 12 111320-006 12 111420-006 20 MC 111520-006
12 111120-007 16 111320-007 14 MC 111420-007 25 111520-007
16 111120-008 20 MC 111320-008 16 111420-008 30 111520-008

18 MC 111420-009 36 111520-009
20 111420-010 40 MC 111520-010
28 MC 111420-011
30 111420-012

PHERO-sub 0710-E PHERO-sub 1010-E PHERO-sub 1515-E PHERO-sub 2020-E

No. of
Samples

Part Number No. of

Samples

Part Number No. of

Samples

Part Number No. of

Samples

Part Number

1mm Thick Combs

1 111121-001 1 111321-001 1 111421-001 1 111521-001
2 111121-002 2 111321-002 2 111421-002 2 111521-002
4 111121-003 4 111321-003 4 111421-003 4 111521-003
8 111121-004 8 111321-004 10 111421-004 10 111521-004
8 MC 111121-005 10 MC 111321-005 10 MC 111421-005 16 111521-005
10 111121-006 12 111321-006 12 111421-006 20 MC 111521-006
12 111121-007 16 111321-007 14 MC 111421-007 25 111521-007
16 111121-008 20 MC 111321-008 16 111421-008 30 111521-008

18 MC 111421-009 36 111521-009
20 111421-010 40 MC 111521-010
28 MC 111421-011
30 111421-012

17

Manual

PHERO-sub E series

BIOTEC-FISCHER GmbH
Daimlerstrasse 6
35447 Reiskirchen / Germany
Fon: [phone]
Fax: [phone]
e-mail: [e-mail]
Revision: 1.0

PHERO-sub 0710-E PHERO-sub 1010-E PHERO-sub 1515-E PHERO-sub 2020-E

No. of
Samples

Part Number No. of

Samples

Part Number No. of

Samples

Part Number No. of

Samples

Part Number

1.5mm Thick Combs

1 111122-001 1 111322-001 1 111422-001 1 111522-001
2 111122-002 2 111322-002 2 111422-002 2 111522-002
4 111122-003 4 111322-003 4 111422-003 4 111522-003
8 111122-004 8 111322-004 10 111422-004 10 111522-004
8 MC 111122-005 10 MC 111322-005 10 MC 111422-005 16 111522-005
10 111122-006 12 111322-006 12 111422-006 20 MC 111522-006
12 111122-007 16 111322-007 14 MC 111422-007 25 111522-007
16 111122-008 20 MC 111322-008 16 111422-008 30 111522-008

28 MC 111422-009 36 111522-009
20 111422-010 40 MC 111522-010
28 111422-011
30 111422-012

PHERO-sub 0710-E PHERO-sub 1010-E PHERO-sub 1515-E PHERO-sub 2020-E

No. of
Samples

Part Number No. of

Samples

Part Number No. of

Samples

Part Number No. of

Samples

Part Number

2mm Thick Combs

1 111123-001 1 111323-001 1 111423-001 1 111523-001
2 111123-002 2 111323-002 2 111423-002 2 111523-002
4 111123-003 4 111323-003 4 111423-003 4 111523-003
8 111123-004 8 111323-004 10 111423-004 10 111523-004
8 MC 111123-005 10 MC 111323-005 10 MC 111423-005 16 111523-005
10 111123-006 12 111323-006 12 111423-006 20 MC 111523-006
12 111123-007 16 111323-007 14 MC 111423-007 25 111523-007
16 111123-008 20 MC 111323-008 16 111423-008 30 111523-008

18 MC 111423-009 36 111523-009
20 111423-010 40 MC 111523-010
28 MC 111423-011
30 111423-012

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Manual

PHERO-sub E series

BIOTEC-FISCHER GmbH
Daimlerstrasse 6
35447 Reiskirchen / Germany
Fon: [phone]
Fax: [phone]
e-mail: [e-mail]
Revision: 1.0

PHERO-sub 2632-E Combs:

Description Part Number

Comb 28 sample MC, 0.75mm thick 111620-001
Comb 56 sample MC, 0.75mm thick 111620-002
Comb 28 sample MC, 1mm thick 111621-001
Comb 56 sample MC, 1mm thick 111621-002
Comb 28 sample MC, 1.5mm thick 111622-001
Comb 56 sample MC, 1.5mm thick 111622-002
Comb 28 sample MC, 2mm thick 111623-001
Comb 56 sample MC, 2mm thick 111623-002

PHERO-sub 1008-E Combs:-

Description Part Number

Comb 1 sample , 1 dye , 1mm thick 111220-001
Comb 4 sample, 1mm thick 111220-002
Comb 8 sample, 1mm thick 111220-003
Comb 12 sample, 1mm thick 111220-004
Comb 16 sample, 1mm thick 111220-005
Comb 20 sample MC, 1mm thick 111220-006
Comb 1 sample , 1 dye , 1.5mm thick 111221-001
Comb 4 sample, 1.5mm thick 111221-002
Comb 8 sample, 1.5mm thick 111221-003
Comb 12 sample, 1.5mm thick 111221-004
Comb 16 sample, 1.5mm thick 111221-005
Comb 20 sample MC, 1.5mm thick 111221-006

19

Manual

PHERO-sub E series

BIOTEC-FISCHER GmbH
Daimlerstrasse 6
35447 Reiskirchen / Germany
Fon: [phone]
Fax: [phone]
e-mail: [e-mail]
Revision: 1.0

Warranty

The multiSUB Horizontal Electrophoresis units have a warranty against manufacturing

and material faults of twelve months from date of customer receipt.

If any defects occur during this warranty period, your supplier will repair or replace the

defective parts free of charge.

This warranty does not cover defects occurring by accident or misuse or defects

caused by improper operation.

Units where repair or modification has been performed by anyone other than your

supplier or an appointed distributor or representative are no longer under warranty

from the time the unit was modified.

Units which have accessories or repaired parts not supplied by your supplier or it’s

associated distributors have invalidated warranty.

Your supplier cannot repair or replace free of charge units where improper solutions or

chemicals have been used. For a list of these please see the Care and Maintenance

subsection.

If a problem does occur then please contact your supplier.

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