Eppendorf Thermomixer R User Manual
55 900.012-12/1008
Thermomixer comfort /
Thermomixer R
Operating Manual
Copyright
©
2007 Eppendorf AG, Hamburg. No part of this publication may be reproduced without
the prior permission of the copyright owner.
Trademarks
eppendorf
®
and eppendorf Thermomixer® are registered trademarks of Eppendorf AG,
Hamburg, Germany.
Thermomixer is a trademark of Eppendorf AG, Hamburg, Germany.
Falcon™ is a registered trademark of Becton Dickinson, Franklin Lakes, USA.
Trademarks are not marked in all cases with ™ or
®
in this manual.
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5355 900.012-13/1109
Thermomixer comfort / Thermomixer R — Operating Manual
Table of contents
Table of contents
1 User instructions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.1 Using this manual. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.2 Warning signs and hazard icons . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.3 Symbols used. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.4 Abbreviations used. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
1.5 Glossary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
2 Product description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
2.1 Main illustration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
2.2 Delivery package . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
2.3 Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
2.3.1 Thermomixer comfort / Thermomixer R . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
2.3.2 IsoTherm system (optional accessory) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
3 Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
3.1 Intended use. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
3.2 User profile. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
3.3 Note on product liability . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
3.4 Warnings for intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
3.5 Warning and instruction signs on the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
4 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
4.1 Preparing installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
4.2 Selecting location . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
4.3 Installing instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
5 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
5.1 Overview of operating controls. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
5.2 Inserting exchangeable thermoblocks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
5.2.1 Exchangeable thermoblocks for micro test tubes . . . . . . . . . . . . . . . . . . . . . . 19
5.2.2 MTP exchangeable thermoblock . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
5.2.3 Adapter plate for 0.2 ml tubes or 96 Well PCR plates. . . . . . . . . . . . . . . . . . . 20
5.2.4 Exchangeable thermoblocks for slides and slides DC . . . . . . . . . . . . . . . . . . 20
5.3 Inserting tubes and plates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
5.4 Time counting mode. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
5.5 Temperature control and mixing. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
5.5.1 Basic temperature control. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
5.5.2 Operation without temperature control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
5.5.3 Mixing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
5.5.4 Short Mix. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
5.5.5 Interrupting operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
5.5.6 Interval Mix . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
5.5.7 Programmable temperature control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
5.5.8 Save the values. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
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Operating Manual
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Thermomixer comfort / Thermomixer R — Operating Manual
Table of contents
EN
6 Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
6.1 General errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
6.2 Error messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
7 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
7.1 Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
7.2 Disinfection / Decontamination. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
7.3 Decontamination before shipping. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
7.4 Temperature control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
8 Transport, storage and disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
Operating Manual
8.1 Transport . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
8.2 Storage. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
8.3 Disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
9 Technical data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
9.1 Power supply . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
9.2 Ambient conditions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
9.3 Weight / dimensions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
9.4 Application parameters. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
9.5 Additional data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
10 Ordering information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
10.1 Thermomixer comfort / Thermomixer R . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
10.2 Exchangeable thermoblocks and adapter plates . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
10.3 Tubes and plates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
10.4 IsoTherm-System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
10.5 Temperature sensor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
11 Index. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
9.5.1 Interfaces . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
9.5.2 Time interval . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
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Thermomixer comfort / Thermomixer R — Operating Manual
1 User instructions
1 User instructions
1.1 Using this manual
EN
Before using the device for the first time, please read the operating manual.
Please view this operating manual as part of the product and keep it somewhere easily
accessible.
If the device is transferred to a third party, include this operating manual.
1.2 Warning signs and hazard icons
Depiction Meaning
DANGER
Risk of electric shock with potential for severe injury or death as a
consequence.
DANGER
Risk of explosion with potential for severe injury or death as a
consequence.
WARNING
Warning of potential injury or health risk.
CAUTION
Refers to risk of damage to property.
Refers to particularly useful information and tips.
1.3 Symbols used
Depiction Meaning
1.
2.
•
You are requested to perform an action.
Perform these actions in the sequence described.
List.
Operating Manual
Text Terms from the device display.
7
Thermomixer comfort / Thermomixer R — Operating Manual
1 User instructions
EN
1.4 Abbreviations used
1.5 Glossary
Deepwell plate Plate with 48, 96 or 384 wells with a larger volume than
Operating Manual
Microplate Plates with 24, 48, 96 or 384 wells for the preparation,
semi-skirted PCR
plate
skirted PCR plate PCR plate with a surrounding edge.
unskirted PCR plate PCR plate without a surrounding edge.
Well Cavity. Microplate, PCR plate or Deepwell plate tube.
DWP Deepwell plate
MTP Microplate
PCR Polymerase Chain Reaction
rpm Revolutions per minute
microplates. Suitable for the preparation, mixing,
centrifuging, transporting and storing of solid and liquid
samples.
mixing, centrifuging, transporting and storing of solid
and liquid samples.
PCR plate with surrounding half-edge.
8
Thermomixer comfort / Thermomixer R — Operating Manual
1 2 3
547
6
2 Product description
2 Product description
2.1 Main illustration
Abb. 1:Thermomixer c ompact
Fig. 1: Thermomixer compact
1 Display 2 Thermosocket
3 Operating controls 4 RS 232 interface
5 Mains connection socket 6 Fuse connector
7 Mains switch
EN
Operating Manual
2.2 Delivery package
Quant
ity
or - 022670107
1- - Mains cable
1- - Hexagon socket key
1 5355 900.012 - Operating Manual Thermomixer comfort /
Order No.
(International)
5355 000.011 -
Order No.
(North
America)
Description
Thermomixer comfort
without exchangeable thermoblock
Thermomixer R
without exchangeable thermoblock
Thermomixer R
9
Thermomixer comfort / Thermomixer R — Operating Manual
2 Product description
EN
2.3 Features
2.3.1 Thermomixer comfort / Thermomixer R
The Thermomixer comfort / Thermomixer R offers many options for temperature control and
mixing of liquids in the microliter and milliliter ranges:
• In exchangeable thermoblocks liquids can be simultaneously mixed and
temperature-controlled
– in 0.2, 0.5, 1.5, 2.0 ml micro test tubes (e.g. Eppendorf Safe-Lock and PCR tubes),
– in Falcon tubes 15 and 50 ml,
– in 1.5 and 2 ml Cryo tubes,
Operating Manual
– in 5 ml test tubes made from plastic or glass
– as well as MTP or DWP with any bottom contours, incl. PCR plates
• Cooling down the samples to 13 °C below room temperature is achieved by Peltier cooling.
Two special functions extend the area of application of theThermomixer comfort / Thermomixer R
• Interval mixing:
Continuous alternation between mixing phase and standstill where mixing frequency and
duration of the mixing and pause functions can be freely selected.
• Program mode:
In addition to a normal mixing/temperature control run two combined, consecutive mixing/
temperature control runs can be freely programmed. When the first run is completed, the
second run will switch on automatically so that two different treatments of the same liquid can
be carried out.
With the Eppendorf IsoRack, a total of 24 micro test tubes can be placed on an exchangeable
thermoblock. The IsoRack is part of the IsoTherm system. This means that with the Thermomixer
comfort / Thermomixer R and the IsoTherm system, micro test tubes can be handled on the lab
bench at all the temperatures that are relevant for everyday lab use.
The robust, space-saving design of the Thermomixer comfort / Thermomixer R combined with
the chemical-resistant plastic housing and the keypad make this Eppendorf device another
professional companion in your daily routine.
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Thermomixer comfort / Thermomixer R — Operating Manual
2 Product description
2.3.2 IsoTherm system (optional accessory)
With the IsoTherm system the samples can be cooled efficiently and at a constant temperature
for several hours and then be thawed gently.
In addition, the tube holder serves as a working aid during the filling of micro test tubes and the
preparation, sorting, distribution, transporting, storing and freezing of samples in micro test
tubes.
The IsoTherm system includes the components IsoRack, IsoPack, IsoSafe and PCR-Cooler.
Features
• IsoPack and IsoSafe
Cooling batteries for -21°C and 0 °C which allow temperature-sensitive samples to be
stored for hours on the lab bench in micro test tubes and in a corresponding insulated box
(IsoSafe).
• IsoRack
Tube holder for 24 micro test tubes (0.5 ml or 1.5/2.0 ml ) each to transfer the tubes from the
cooling battery to the exchangeable thermoblocks. The lockable and stackable,
snap-together IsoRacks are also particularly suited to the storage of micro test tubes in
refrigerators or freezers. The IsoRacks can be autoclaved. The spacing of the test tubes in
the IsoRack is determined by the use of multi-channel pipettes.
• PCR-Cooler
Cool pack for 0 °C for the storage of PCR tubes or plates (semi-skirted, unskirted and
skirted) on the lab bench and in an insulated box (IsoSafe).
Further details can be found on our homepage www.eppendorf.com and in the
operating manual for the IsoTherm system.
Hint!
EN
Operating Manual
11
3 Safety
3Safety
EN
3.1 Intended use
The Thermomixer comfort / Thermomixer R is intended exclusively for indoor use and is for the
temperature control and mixing of aqueous solutions in closed micro test tubes and plates.
Only use Eppendorf accessories or accessories recommended by Eppendorf.
3.2 User profile
This device must only be used by skilled personnel with the appropriate training.
Before using the device, read the operating manual carefully and familiarize yourself with the
device's mode of operation.
Operating Manual
3.3 Note on product liability
In the following cases the protection provided in the device may be impaired. The liability for the
function of the device passes to the operator if:
• The device is not used in accordance with the operating manual.
• The device is used outside the range of application described herein.
• The device is used with accessories or consumables (e.g. tubes and plates) which are not
recommended by Eppendorf.
• The device is maintained or repaired by persons not authorized by Eppendorf.
• The owner has made unauthorized modifications to the device.
Thermomixer comfort / Thermomixer R — Operating Manual
3.4 Warnings for intended use
Please read the operating manual and note the following general safety instructions before using
the Thermomixer comfort / Thermomixer R.
Risk of explosion!
Do not operate the device in rooms where work is being carried out with explosive
DANGER!
substances.
Do not use this device to process any explosive or highly reactive substances.
Do not use this device to process any substances which could create an explosive
atmosphere.
Danger! Electric shock from damage to device/power cable.
Only switch on the device if the device and the power cable are undamaged.
DANGER!
Only use devices that have been properly installed or repaired.
Danger! Lethal voltages inside the device.
Ensure that the housing is always closed and undamaged so that no parts inside
DANGER!
the device can be contacted by accident.
Do not remove the housing of the device.
Do not allow the device to be opened by anyone except service personnel who have
been specifically authorized by Eppendorf.
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Thermomixer comfort / Thermomixer R — Operating Manual
3 Safety
Danger! Electric shock as a result of penetration of liquid.
Switch off the device and disconnect it from the power supply before starting
DANGER!
DANGER!
DANGER!
cleaning or disinfecting.
Do not allow any liquids to enter the inside of the housing.
Do not perform spay disinfection.
Only reconnect the device to the power supply once it is completely dry.
Danger! Damage to health from biologically or chemically hazardous substances.
Hazardous chemicals cause burns and other health hazards.
Observe the material safety data sheets for the biological and chemical substances used.
Wear personal protective equipment (PPE) at all times when working with biological or
chemical substances.
Follow the instructions for cleaning and decontamination, and ensure that hygiene safety
standards are maintained.
Risk when handling toxic or radioactively-marked liquids or pathogenic germs.
Follow national regulations governing the handling of these substances.
For complete instructions regarding the handling of germs or biological material of
risk group II or higher, please refer to the "Laboratory Biosafety Manual" (Source:
World Health Organization, current edition of the Laboratory Biosafety Manual).
EN
Operating Manual
Warning! Damage due to incorrect power supply.
Only connect the device to power sources that match the electrical specifications on
WARNING!
the device's nameplate.
Use only sockets with protective earth.
Warning! Risk of burns from hot surfaces.
The exchangeable thermoblock and the thermal base can be very hot after heating and
cause burns.
WARNING!
Allow heated exchangeable thermoblocks and the thermal base to cool down
completely before removing the exchangeable thermoblock.
Warning! Risk of injury through chemical or mechanical damage to exchangeable
thermoblocks.
Check the exchangeable thermoblocks regularly.
WARNING!
Do not use exchangeable thermoblocks that show signs of corrosion or mechanical
damage.
Warning! Poor safety due to incorrect accessories and spare parts.
The use of accessories and spare parts other than those recommended by Eppendorf
may impair the safety, function and precision of the device. Eppendorf accepts no
WARNING!
warranty or liability for damage caused by non-recommended accessories and spare
parts or incorrect use.
Use only accessories recommended by Eppendorf and original spare parts.
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