Eppendorf ThermoMixer FP User Manual
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Eppendorf ThermoMixer®
F0.5/F1.5/F2.0/FP
Operating manual
Copyright ©2014 Eppendorf AG, Hamburg, Germany. All rights reserved, including
graphics and images. No part of this publication may be reproduced without the prior
permission of the copyright owner.
®
Eppendorf
and the Eppendorf logo are registered trademarks of Eppendorf AG,
Hamburg, Germany.
®
Eppendorf ThermoMixer
, Eppendorf ThermoTop®, and condens.protect® are registered
trademarks of Eppendorf AG, Hamburg, Germany.
®
Registered trademarks and protected trademarks are not marked in all cases with
or ™
in this manual.
Operating manual Eppendorf ThermoMixer® F0.5/F1.5/F2.0/FPseeon p.Fig.Tab.p.
English (EN)Operating manual
5386 900.002-01/122013
Table of contents
®
Eppendorf ThermoMixer
F0.5/F1.5/F2.0/FP
English (EN)
Table of contents
1 Operating instructions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.1 Using this manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.2 Danger symbols and danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.2.1 Danger symbols. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.2.2 Danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.3 Symbols used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
1.4 Abbreviations used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
1.5 Glossary. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
2 Product description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
2.1 Main illustration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
2.2 Delivery package . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
2.2.1 Eppendorf ThermoMixer F0.5, Eppendorf ThermoMixer F1.5,
Eppendorf ThermoMixer F2.0. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
2.2.2 Eppendorf ThermoMixer FP . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
2.3 Features. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
3 Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
3.1 Intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
3.2 User profile . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
3.3 Information on product liability . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
3.4 Warnings for intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
3.5 Danger symbols on the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
3
4 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
4.1 Selecting the location . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
4.2 Installing the instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
5 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
5.1 Overview of operating controls . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
5.2 Inserting tubes and plates. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
5.3 Installing the ThermoTop . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
5.4 Mixing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
5.4.1 Mixing without temperature control . . . . . . . . . . . . . . . . . . . . . . . 20
5.4.2 Mixing and tempering . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
5.4.3 Short Mix . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
5.5 Temperature control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
5.5.1 Temperature control without mixing process . . . . . . . . . . . . . . . . 21
5.6 Menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
5.6.1 Navigate the menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
5.6.2 Menu structure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
5.7 Loading saved parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
Table of contents
4
Eppendorf ThermoMixer
®
F0.5/F1.5/F2.0/FP
English (EN)
6 Troubleshooting. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
6.1 General errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
6.2 Error messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
7 Maintenance. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
7.1 Setting service intervals . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
7.2 Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
7.3 Disinfection/Decontamination. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
7.4 Decontamination before shipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
7.5 Verification of temperature control. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
8 Transport, storage and disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
8.1 Transport. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
8.2 Storage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
8.3 Disposal. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
9 Technical data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
9.1 Power supply. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
9.2 Weight/dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
9.3 Ambient conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
9.4 Application parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
9.4.1 Temperature control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
9.4.2 Mix. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
9.5 Interface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
10 Ordering information. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
10.1 Device and accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
10.2 Tubes and plates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
10.3 Temperature Verification System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
Certificates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
Operating instructions
®
Eppendorf ThermoMixer
F0.5/F1.5/F2.0/FP
English (EN)
1 Operating instructions
1.1 Using this manual
Read this operating manual completely before using the device for the first time. Also
observe the instructions for use of the accessories.
This operating manual is part of the product. Thus, it must always be easily accessible.
Enclose this operating manual when transferring the device to third parties.
You will find the current version of the operating manual for all available languages on
our webpage under
1.2 Danger symbols and danger levels
The safety precautions in these instructions have the following danger symbols and
danger levels:
1.2.1 Danger symbols
Biohazard Explosion
Electric shock Hot surface
www.eppendorf.com.
5
Hazard point Risk of fire
Crushing Material damage
1.2.2 Danger levels
DANGER Will lead to severe injuries or death.
WARNING Can lead to severe injuries or death.
CAUTION May lead to light to moderate injuries.
NOTICE May lead to material damage.
Operating instructions
6
Eppendorf ThermoMixer
®
F0.5/F1.5/F2.0/FP
English (EN)
1.3 Symbols used
Depiction Meaning
1.
Actions in the specified order
2.
Actions without a specified order
• List
Text Display text or software text
Additional information
1.4 Abbreviations used
PCR
Polymerase chain reaction
rpm
Revolutions per minute – in rpm
1.5 Glossary
Deepwell plate Plate with 48, 96 or 384 wells with a larger volume than
microplates. Suitable for the preparation, mixing,
centrifuging, transporting and storing of solid and liquid
samples.
Lid Lid for the thermoblock. Ensures uniform temperature
control and protects samples from unwanted exposure
to light.
Microplate Plate with 24, 48, 96 or 384 wells for the preparation,
mixing, centrifuging, transporting and storing of solid
and liquid samples.
PCR plate Plate with 96 or 384 wells for PCR applications.
ThermoTop Heated cover for the thermoblock. Prevents the
formation of condensation on the inner wall or the lid of
the tube thanks to the condens.protect technology.
Well Concave vessel of a microplate, PCR plate or deepwell
plate.
Product description
®
Eppendorf ThermoMixer
F0.5/F1.5/F2.0/FP
English (EN)
2 Product description
2.1 Main illustration
Abb. 2-1:ThermoMixer F1.5 (ThermoMixer F0.5 and ThermoMixer F2.0 are similar)
1 33 542
674
Fig. 2-1: ThermoMixer F1.5 (ThermoMixer F0.5 and ThermoMixer F2.0 are similar)
Abb. 2-2:ThermoMixer FP
1 3 3 542
7
Fig. 2-2: ThermoMixer FP
674
Product description
8
Eppendorf ThermoMixer
English (EN)
®
F0.5/F1.5/F2.0/FP
1 Operating controls
2 Display
3 Thermoblock
4 Centering pins
5 Mains/power switch
6 Mains/power cord socket
7 USB interface (for Eppendorf Service
only)
2.2 Delivery package
2.2.1 Eppendorf ThermoMixer F0.5, Eppendorf ThermoMixer F1.5, Eppendorf ThermoMixer F2.0
Quantity Order no.
(International)
Order no.
(North America)
Description
1 5386 000.010 5386000028 ThermoMixer F0.5
with thermoblock for 24 0.5 mL tubes
or 1 5384 000.012 5384000020 ThermoMixer F1.5
with thermoblock for 24 1.5 mL tubes
or 1 5387 000.013 5387000021 ThermoMixer F2.0
with thermoblock for 24 2.0 mL tubes
1– – Mains/power cord
1 5384 900.013 Operating Manual
ThermoMixer F0.5/F1.5/F2.0/FP
1 5384 900.021 Short Instructions
ThermoMixer F0.5/F1.5/F2.0/FP
1– – Certificate of Quality
2.2.2 Eppendorf ThermoMixer FP
Quantity Order no.
(International)
Order no.
(North America)
Description
1 5385 000.016 5385000024 ThermoMixer FP
with thermoblock for microplates and
Deepwell plates
1– – Mains/power cord
1 5363 000.233 5363000233 Lid
for ThermoMixer F0.5/F1.5/F2.0/FP
1 5384 900.013 Operating Manual
ThermoMixer F0.5/F1.5/F2.0/FP
1 5384 900.021 Short Instructions
ThermoMixer F0.5/F1.5/F2.0/FP
1– – Certificate of Quality
Product description
®
Eppendorf ThermoMixer
F0.5/F1.5/F2.0/FP
English (EN)
Check the delivery for completeness.
Check all parts for damage in transit.
To safely transport and store the device, keep the transport box and packing
material.
2.3 Features
You can use the Eppendorf ThermoMixer F0.5/F1.5/F2.0/FP for performing two basic
applications of the sample preparation in one convenient workstep: the simultaneous
mixing and tempering of the sample material.
• Eppendorf ThermoMixer F0.5: for 24 0.5 mL micro test tubes (e.g., Eppendorf
Safe-Lock Tubes)
• Eppendorf ThermoMixer F1.5: for 24 1.5 mL micro test tubes (e.g., Eppendorf
Safe-Lock Tubes)
• Eppendorf ThermoMixer F2.0: for 24 2.0 mL micro test tubes (e.g., Eppendorf
Safe-Lock Tubes)
• Eppendorf ThermoMixer FP: all current plate formats (e.g. Eppendorf Microplates or
Eppendorf Deepwell Plates).
Temperature control
• Temperatures from 4 °C above ambient temperature until 100 °C are met exactly and
constantly.
• The temperatures 37 °C, 42 °C, 56 °C, 95 °C can be selected directly.
Mix
• Anti-spill technology prevents lid wetting and cross contamination.
• Eppendorf ThermoMixer F1.5, Eppendorf ThermoMixer F2.0: You can select mixing
frequencies between 300 rpm and 1 500 rpm.
• Eppendorf ThermoMixer F0.5, Eppendorf ThermoMixer FP: You can select mixing
frequencies between 300 rpm and 2 000 rpm.
2D
• The controlled and efficient mixing movement of the
provides for a fast and complete mixing even of minimum volumes.
• Short Mix: Short, uncomplicated mixing of sample material. The mixing process is
performed at the selected speed as long as you press the short key.
Lid and ThermoTop
• The Lid ensures uniform temperature control and protects samples from unwanted
exposure to light.
• The ThermoTop prevents the formation of condensation on the inner wall or the lid of
the tube thanks to the condens.protect technology.
Mix-Control technology
9
Safety
10
Eppendorf ThermoMixer
®
F0.5/F1.5/F2.0/FP
English (EN)
3 Safety
3.1 Intended use
The Eppendorf ThermoMixer F0.5/F1.5/F2.0/FP is intended for use in a molecular biology
laboratory.
The Eppendorf ThermoMixer F0.5/F1.5/F2.0/FP is designed for the temperature control
and mixing of liquids in closed tubes and closed plates for the preparation and processing
of samples.
The Eppendorf ThermoMixer F0.5/F1.5/F2.0/FP is exclusively intended for use indoors.
All country-specific safety requirements for operating electrical equipment in the
laboratory must be observed.
Only use Eppendorf accessories or accessories recommended by Eppendorf.
3.2 User profile
The device and accessories may only be operated by trained and skilled personnel.
Before using the device, read the operating manual carefully and familiarize yourself with
the device's mode of operation.
3.3 Information on product liability
In the following cases, the designated protection of the device may be compromised.
Liability for any resulting property damage or personal injury is then transferred to the
operator:
• The device is not used in accordance with the operating manual.
• The device is used outside of its intended use.
• The device is used with accessories or consumables which are not recommended by
Eppendorf.
• The device is maintained or repaired by people not authorized by Eppendorf.
• The user makes unauthorized changes to the device.
®
Eppendorf ThermoMixer
3.4 Warnings for intended use
Read the operating instructions and observe the following general safety information
before using the Eppendorf ThermoMixer F0.5/F1.5/F2.0/FP.
DANGER! Risk of explosion.
F0.5/F1.5/F2.0/FP
Safety
11
English (EN)
Do not operate the device in areas where work is completed with explosive
substances.
Do not use this device to process any explosive or highly reactive substances.
Do not use this device for processing any substances which could generate an
explosive atmosphere.
DANGER! Electric shock as a result of penetration of liquid.
Switch off the device and disconnect the power plug before starting cleaning
or disinfection work.
Do not allow any liquids to penetrate the inside of the housing.
Use closed tubes and closed plates.
Do not spray clean/spray disinfect the housing.
Only plug the device back in if it is completely dry, both inside and outside.
WARNING! Electric shock due to damage to device or mains cable.
Only switch on the device if the device and mains cable are undamaged.
Only use devices that have been properly installed or repaired.
In case of danger, disconnect the device from the mains supply by pulling the
power plug from the device or the mains socket or, by using the isolating
device intended for this purpose (e.g., emergency stop switch in the
laboratory).
WARNING! Lethal voltages inside the device.
Ensure that the housing is always closed and undamaged so that no parts
inside the device can be contacted by accident.
Do not remove the housing of the device.
Do not allow any liquids to penetrate the inside of the housing.
Do not allow the device to be opened by anyone except service personnel who
have been specifically authorized by Eppendorf.
Safety
12
Eppendorf ThermoMixer
English (EN)
WARNING! Risk from incorrect supply voltage
Only connect the device to voltage sources which correspond to the electrical
requirements on the name plate.
Only use sockets with a protective earth (PE) conductor and suitable power
cable.
WARNING! Risk of burns from hot surfaces.
The thermoblock can be very hot after heating and cause burns.
Avoid direct contact with a heated thermoblock.
WARNING! Damage to health due to infectious liquids and pathogenic
germs.
When handling infectious liquids and pathogenic germs, observe the national
regulations, the biological security level of your laboratory, the material safety
data sheets, and the manufacturer's application notes.
Wear personal protective equipment.
For full instructions regarding the handling of germs or biological material of
risk group II or higher, please refer to the "Laboratory Biosafety Manual"
(Source: World Health Organization, current edition of the Laboratory
Biosafety Manual).
®
F0.5/F1.5/F2.0/FP
WARNING! Risk of fire.
Do not use this device to process any highly flammable liquids.
WARNING! Damage to health due to contaminated device and accessories.
Decontaminate the device and the accessories before storage and shipping.
WARNING! Risk of injury due to incorrect consumables.
• Poorly fitting tubes or plates can become detached from the thermoblock.
• Glass tubes can smash.
Only use the thermoblocks with the consumables designed for them.
Never use tubes made of glass or other fragile material.
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