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Eppendorf ThermoMixer®
F0.5/F1.5/F2.0/FP
Operating manual
Copyright ©2014 Eppendorf AG, Hamburg, Germany. All rights reserved, including
graphics and images. No part of this publication may be reproduced without the prior
permission of the copyright owner.
®
Eppendorf
and the Eppendorf logo are registered trademarks of Eppendorf AG,
Hamburg, Germany.
®
Eppendorf ThermoMixer
, Eppendorf ThermoTop®, and condens.protect® are registered
trademarks of Eppendorf AG, Hamburg, Germany.
®
Registered trademarks and protected trademarks are not marked in all cases with
or ™
in this manual.
Operating manual Eppendorf ThermoMixer® F0.5/F1.5/F2.0/FPseeon p.Fig.Tab.p.
English (EN)Operating manual
5386 900.002-01/122013
Table of contents
®
Eppendorf ThermoMixer
F0.5/F1.5/F2.0/FP
English (EN)
Table of contents
1 Operating instructions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.1 Using this manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.2 Danger symbols and danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.2.1 Danger symbols. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.2.2 Danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.3 Symbols used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
1.4 Abbreviations used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
1.5 Glossary. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
2 Product description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
2.1 Main illustration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
2.2 Delivery package . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
2.2.1 Eppendorf ThermoMixer F0.5, Eppendorf ThermoMixer F1.5,
Eppendorf ThermoMixer F2.0. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
2.2.2 Eppendorf ThermoMixer FP . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
2.3 Features. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
3 Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
3.1 Intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
3.2 User profile . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
3.3 Information on product liability . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
3.4 Warnings for intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
3.5 Danger symbols on the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
3
4 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
4.1 Selecting the location . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
4.2 Installing the instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
5 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
5.1 Overview of operating controls . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
5.2 Inserting tubes and plates. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
5.3 Installing the ThermoTop . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
5.4 Mixing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
5.4.1 Mixing without temperature control . . . . . . . . . . . . . . . . . . . . . . . 20
5.4.2 Mixing and tempering . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
5.4.3 Short Mix . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
5.5 Temperature control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
5.5.1 Temperature control without mixing process . . . . . . . . . . . . . . . . 21
5.6 Menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
5.6.1 Navigate the menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
5.6.2 Menu structure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
5.7 Loading saved parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
Table of contents
4
Eppendorf ThermoMixer
®
F0.5/F1.5/F2.0/FP
English (EN)
6 Troubleshooting. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
6.1 General errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
6.2 Error messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
7 Maintenance. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
7.1 Setting service intervals . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
7.2 Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
7.3 Disinfection/Decontamination. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
7.4 Decontamination before shipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
7.5 Verification of temperature control. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
8 Transport, storage and disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
8.1 Transport. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
8.2 Storage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
8.3 Disposal. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
9 Technical data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
9.1 Power supply. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
9.2 Weight/dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
9.3 Ambient conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
9.4 Application parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
9.4.1 Temperature control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
9.4.2 Mix. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
9.5 Interface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
10 Ordering information. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
10.1 Device and accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
10.2 Tubes and plates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
10.3 Temperature Verification System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
Certificates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
Operating instructions
®
Eppendorf ThermoMixer
F0.5/F1.5/F2.0/FP
English (EN)
1 Operating instructions
1.1 Using this manual
Read this operating manual completely before using the device for the first time. Also
observe the instructions for use of the accessories.
This operating manual is part of the product. Thus, it must always be easily accessible.
Enclose this operating manual when transferring the device to third parties.
You will find the current version of the operating manual for all available languages on
our webpage under
1.2 Danger symbols and danger levels
The safety precautions in these instructions have the following danger symbols and
danger levels:
1.2.1 Danger symbols
Biohazard Explosion
Electric shock Hot surface
www.eppendorf.com.
5
Hazard point Risk of fire
Crushing Material damage
1.2.2 Danger levels
DANGER Will lead to severe injuries or death.
WARNING Can lead to severe injuries or death.
CAUTION May lead to light to moderate injuries.
NOTICE May lead to material damage.
Operating instructions
6
Eppendorf ThermoMixer
®
F0.5/F1.5/F2.0/FP
English (EN)
1.3 Symbols used
Depiction Meaning
1.
Actions in the specified order
2.
Actions without a specified order
• List
Text Display text or software text
Additional information
1.4 Abbreviations used
PCR
Polymerase chain reaction
rpm
Revolutions per minute – in rpm
1.5 Glossary
Deepwell plate Plate with 48, 96 or 384 wells with a larger volume than
microplates. Suitable for the preparation, mixing,
centrifuging, transporting and storing of solid and liquid
samples.
Lid Lid for the thermoblock. Ensures uniform temperature
control and protects samples from unwanted exposure
to light.
Microplate Plate with 24, 48, 96 or 384 wells for the preparation,
mixing, centrifuging, transporting and storing of solid
and liquid samples.
PCR plate Plate with 96 or 384 wells for PCR applications.
ThermoTop Heated cover for the thermoblock. Prevents the
formation of condensation on the inner wall or the lid of
the tube thanks to the condens.protect technology.
Well Concave vessel of a microplate, PCR plate or deepwell
plate.
Product description
®
Eppendorf ThermoMixer
F0.5/F1.5/F2.0/FP
English (EN)
2 Product description
2.1 Main illustration
Abb. 2-1:ThermoMixer F1.5 (ThermoMixer F0.5 and ThermoMixer F2.0 are similar)
1 33 542
674
Fig. 2-1: ThermoMixer F1.5 (ThermoMixer F0.5 and ThermoMixer F2.0 are similar)
Abb. 2-2:ThermoMixer FP
1 3 3 542
7
Fig. 2-2: ThermoMixer FP
674
Product description
8
Eppendorf ThermoMixer
English (EN)
®
F0.5/F1.5/F2.0/FP
1 Operating controls
2 Display
3 Thermoblock
4 Centering pins
5 Mains/power switch
6 Mains/power cord socket
7 USB interface (for Eppendorf Service
only)
2.2 Delivery package
2.2.1 Eppendorf ThermoMixer F0.5, Eppendorf ThermoMixer F1.5, Eppendorf ThermoMixer F2.0
Quantity Order no.
(International)
Order no.
(North America)
Description
1 5386 000.010 5386000028 ThermoMixer F0.5
with thermoblock for 24 0.5 mL tubes
or 1 5384 000.012 5384000020 ThermoMixer F1.5
with thermoblock for 24 1.5 mL tubes
or 1 5387 000.013 5387000021 ThermoMixer F2.0
with thermoblock for 24 2.0 mL tubes
1– – Mains/power cord
1 5384 900.013 Operating Manual
ThermoMixer F0.5/F1.5/F2.0/FP
1 5384 900.021 Short Instructions
ThermoMixer F0.5/F1.5/F2.0/FP
1– – Certificate of Quality
2.2.2 Eppendorf ThermoMixer FP
Quantity Order no.
(International)
Order no.
(North America)
Description
1 5385 000.016 5385000024 ThermoMixer FP
with thermoblock for microplates and
Deepwell plates
1– – Mains/power cord
1 5363 000.233 5363000233 Lid
for ThermoMixer F0.5/F1.5/F2.0/FP
1 5384 900.013 Operating Manual
ThermoMixer F0.5/F1.5/F2.0/FP
1 5384 900.021 Short Instructions
ThermoMixer F0.5/F1.5/F2.0/FP
1– – Certificate of Quality
Product description
®
Eppendorf ThermoMixer
F0.5/F1.5/F2.0/FP
English (EN)
Check the delivery for completeness.
Check all parts for damage in transit.
To safely transport and store the device, keep the transport box and packing
material.
2.3 Features
You can use the Eppendorf ThermoMixer F0.5/F1.5/F2.0/FP for performing two basic
applications of the sample preparation in one convenient workstep: the simultaneous
mixing and tempering of the sample material.
• Eppendorf ThermoMixer F0.5: for 24 0.5 mL micro test tubes (e.g., Eppendorf
Safe-Lock Tubes)
• Eppendorf ThermoMixer F1.5: for 24 1.5 mL micro test tubes (e.g., Eppendorf
Safe-Lock Tubes)
• Eppendorf ThermoMixer F2.0: for 24 2.0 mL micro test tubes (e.g., Eppendorf
Safe-Lock Tubes)
• Eppendorf ThermoMixer FP: all current plate formats (e.g. Eppendorf Microplates or
Eppendorf Deepwell Plates).
Temperature control
• Temperatures from 4 °C above ambient temperature until 100 °C are met exactly and
constantly.
• The temperatures 37 °C, 42 °C, 56 °C, 95 °C can be selected directly.
Mix
• Anti-spill technology prevents lid wetting and cross contamination.
• Eppendorf ThermoMixer F1.5, Eppendorf ThermoMixer F2.0: You can select mixing
frequencies between 300 rpm and 1 500 rpm.
• Eppendorf ThermoMixer F0.5, Eppendorf ThermoMixer FP: You can select mixing
frequencies between 300 rpm and 2 000 rpm.
2D
• The controlled and efficient mixing movement of the
provides for a fast and complete mixing even of minimum volumes.
• Short Mix: Short, uncomplicated mixing of sample material. The mixing process is
performed at the selected speed as long as you press the short key.
Lid and ThermoTop
• The Lid ensures uniform temperature control and protects samples from unwanted
exposure to light.
• The ThermoTop prevents the formation of condensation on the inner wall or the lid of
the tube thanks to the condens.protect technology.
Mix-Control technology
9
Safety
10
Eppendorf ThermoMixer
®
F0.5/F1.5/F2.0/FP
English (EN)
3 Safety
3.1 Intended use
The Eppendorf ThermoMixer F0.5/F1.5/F2.0/FP is intended for use in a molecular biology
laboratory.
The Eppendorf ThermoMixer F0.5/F1.5/F2.0/FP is designed for the temperature control
and mixing of liquids in closed tubes and closed plates for the preparation and processing
of samples.
The Eppendorf ThermoMixer F0.5/F1.5/F2.0/FP is exclusively intended for use indoors.
All country-specific safety requirements for operating electrical equipment in the
laboratory must be observed.
Only use Eppendorf accessories or accessories recommended by Eppendorf.
3.2 User profile
The device and accessories may only be operated by trained and skilled personnel.
Before using the device, read the operating manual carefully and familiarize yourself with
the device's mode of operation.
3.3 Information on product liability
In the following cases, the designated protection of the device may be compromised.
Liability for any resulting property damage or personal injury is then transferred to the
operator:
• The device is not used in accordance with the operating manual.
• The device is used outside of its intended use.
• The device is used with accessories or consumables which are not recommended by
Eppendorf.
• The device is maintained or repaired by people not authorized by Eppendorf.
• The user makes unauthorized changes to the device.
®
Eppendorf ThermoMixer
3.4 Warnings for intended use
Read the operating instructions and observe the following general safety information
before using the Eppendorf ThermoMixer F0.5/F1.5/F2.0/FP.
DANGER! Risk of explosion.
F0.5/F1.5/F2.0/FP
Safety
11
English (EN)
Do not operate the device in areas where work is completed with explosive
substances.
Do not use this device to process any explosive or highly reactive substances.
Do not use this device for processing any substances which could generate an
explosive atmosphere.
DANGER! Electric shock as a result of penetration of liquid.
Switch off the device and disconnect the power plug before starting cleaning
or disinfection work.
Do not allow any liquids to penetrate the inside of the housing.
Use closed tubes and closed plates.
Do not spray clean/spray disinfect the housing.
Only plug the device back in if it is completely dry, both inside and outside.
WARNING! Electric shock due to damage to device or mains cable.
Only switch on the device if the device and mains cable are undamaged.
Only use devices that have been properly installed or repaired.
In case of danger, disconnect the device from the mains supply by pulling the
power plug from the device or the mains socket or, by using the isolating
device intended for this purpose (e.g., emergency stop switch in the
laboratory).
WARNING! Lethal voltages inside the device.
Ensure that the housing is always closed and undamaged so that no parts
inside the device can be contacted by accident.
Do not remove the housing of the device.
Do not allow any liquids to penetrate the inside of the housing.
Do not allow the device to be opened by anyone except service personnel who
have been specifically authorized by Eppendorf.
Safety
12
Eppendorf ThermoMixer
English (EN)
WARNING! Risk from incorrect supply voltage
Only connect the device to voltage sources which correspond to the electrical
requirements on the name plate.
Only use sockets with a protective earth (PE) conductor and suitable power
cable.
WARNING! Risk of burns from hot surfaces.
The thermoblock can be very hot after heating and cause burns.
Avoid direct contact with a heated thermoblock.
WARNING! Damage to health due to infectious liquids and pathogenic
germs.
When handling infectious liquids and pathogenic germs, observe the national
regulations, the biological security level of your laboratory, the material safety
data sheets, and the manufacturer's application notes.
Wear personal protective equipment.
For full instructions regarding the handling of germs or biological material of
risk group II or higher, please refer to the "Laboratory Biosafety Manual"
(Source: World Health Organization, current edition of the Laboratory
Biosafety Manual).
®
F0.5/F1.5/F2.0/FP
WARNING! Risk of fire.
Do not use this device to process any highly flammable liquids.
WARNING! Damage to health due to contaminated device and accessories.
Decontaminate the device and the accessories before storage and shipping.
WARNING! Risk of injury due to incorrect consumables.
• Poorly fitting tubes or plates can become detached from the thermoblock.
• Glass tubes can smash.
Only use the thermoblocks with the consumables designed for them.
Never use tubes made of glass or other fragile material.
®
Eppendorf ThermoMixer
WARNING! Contamination due to opening seals of consumables.
In the following cases, the seals of tubes can spring open. Sample material can
escape.
• high vapor pressure of the content
• improperly sealed cover
• damaged sealing lip
• improperly fastened foil
F0.5/F1.5/F2.0/FP
Safety
English (EN)
Always check that consumables have been sealed tightly before use.
WARNING! Injury from sample material being thrown out.
Sample material can be thrown out of open, improperly sealed or unstable tubes
and plates.
Only mix in closed tubes and closed plates.
Observe the nationally prescribed safety environment when working with
hazardous, toxic and pathogenic samples. Pay particular attention to personal
protective equipment (gloves, clothing, goggles etc.), extraction, and the
safety class of the lab.
CAUTION! Poor safety due to incorrect accessories and spare parts.
The use of accessories and spare parts other than those recommended by
Eppendorf may impair the safety, functioning and precision of the device.
Eppendorf cannot be held liable or accept any liability for damage resulting from
the use of incorrect or non-recommended accessories and spare parts, or from
the improper use of such equipment.
Only use accessories and original spare parts recommended by Eppendorf.
13
CAUTION! Crush hazard due to moving parts.
Do not replace any consumables during the mixing process.
Put on the ThermoTop or Lid prior to the mixing process.
Do not remove the ThermoTop or Lid during the mixing process.
NOTICE! Caution! Strong vibration.
When mixing at high speeds, items located near the device may be moved by the
vibrations of the work surface and, e.g., fall off the work table.
Do not place easily movable items near the device or secure them adequately.
Safety
14
Eppendorf ThermoMixer
English (EN)
NOTICE! Damage to the display due to mechanical pressure.
Do not apply mechanical pressure to the display.
NOTICE! Damage from overheating.
Do not install the device near to any heat sources (e.g., heating, drying
cabinet).
Do not expose the device to direct sunlight.
Ensure unobstructed air circulation. Keep free a clearance of at least 10 cm
(3.9 in) around all ventilation grilles.
NOTICE! Damage to electronic components due to condensation.
Condensate can form in the device after it has been moved from a cool
environment to a warmer environment.
After installing the device, wait at least for 3 h. Only then connect the device
to the mains.
NOTICE! Damage from the use of aggressive chemicals.
Do not use any aggressive chemicals on the device or its accessories, such as
strong and weak bases, strong acids, acetone, formaldehyde, halogenated
hydrocarbons or phenol.
If the device has been contaminated by aggressive chemicals, immediately
clean it by means of a mild cleaning agent.
®
F0.5/F1.5/F2.0/FP
3.5 Danger symbols on the device
Representation Meaning Location
Risk of burns from hot surfaces. Upper device
side
Observe the operating manual. Rear of the
device
Caution! Risk of injury from moving
parts.
On the
thermoblock
Observe the operating manual.
Eppendorf ThermoMixer
4 Installation
4.1 Selecting the location
Select the device location according to the following criteria:
• Mains/power connection in accordance with the name plate
• Minimum distance to other devices and walls: 10 cm (3.9 in)
• Resonance free table with horizontal even work surface
• The design of table is suitable for operating the device.
• The design of table is suitable for operating the device.
• Surrounding area must be well ventilated.
• The location must be protected against direct sunlight.
The mains/power switch and cutting unit of the mains/power line must be easily
accessible during operation (e.g., residual current circuit breaker).
4.2 Installing the instrument
WARNING! Risk from incorrect supply voltage
Only connect the device to voltage sources which correspond to the electrical
requirements on the name plate.
Only use sockets with a protective earth (PE) conductor and suitable power
cable.
®
Installation
F0.5/F1.5/F2.0/FP
English (EN)
15
1. Place the Eppendorf ThermoMixer F0.5/F1.5/F2.0/FP on a suitable work surface.
Position the device in such a way that the ventilation slots of the device are not
obstructed.
2. Connect the power cable to the power connection socket of the device and the power
supply.
Operation
16
Eppendorf ThermoMixer
®
F0.5/F1.5/F2.0/FP
English (EN)
5 Operation
5.1 Overview of operating controls
Abb. 5-1:Operating controls
1
2
78
6
Fig. 5-1: Operating controls
1 Temperature keys with control LEDs
Select a temperature or switch off
temperature control
2 short key
Short Mix runs as long as the Taste
short key is being pressed (see p. 21).
3 start/stop key
Start or stop mixing/temperature
control
4 Arrow keys speed
Setting the mixing frequency
Keep the arrow key pressed: Quick
setting
45
3
5 Arrow keys temp
Setting the temperature
Keep the arrow key pressed: Quick
setting
As soon as the target temperature is
modified, the device begins to perform
temperature control.
6 Display
7 Menu arrow key
Navigate the menu: Set the time mode
or the volume
8 menu/enter key
Open menu
Confirm your selection
Eppendorf ThermoMixer
Abb. 5-2:Display Eppendorf ThermoMixer F0.5/F1.5/F2.0/FP
567
Fig. 5-2: Display Eppendorf ThermoMixer F0.5/F1.5/F2.0/FP
®
F0.5/F1.5/F2.0/FP
Operation
English (EN)
1 2 3 4
17
1 Device status
Device is performing mixing/
temperature control.
2 ThermoTop
ThermoTop has been attached.
To prevent condensation, the device
heats up the ThermoTop, before
controlling the temperature of the
thermoblock.
3 Speaker
Speaker switched on.
Speaker switched off.
4 Key lock
Key lock activated: Parameters cannot
be changed.
No key lock.
5 Mixing frequency
6 Actual temperature
When the actual temperature flashes on
the display, the device is not in
temperature control mode operation.
7 Set temperature
When the set temperature has been
reached, only one value is displayed.
Operation
18
Eppendorf ThermoMixer
English (EN)
®
F0.5/F1.5/F2.0/FP
5.2 Inserting tubes and plates
NOTICE! Damage to plates due to too high temperatures.
Polystyrene microplates melt at temperatures above 70 °C.
Polypropylene deepwell plates deform at temperatures above 80 °C. Deformed
plates can become detached from the thermoblock.
Only heat microplates up to 70 °C.
If you are heating deepwell plates above 80 °C, do not exceed the mixing
frequency of 1000 rpm.
NOTICE! Material change of consumables due to extreme temperatures.
Extreme temperatures (e.g., during refrigeration or autoclaving) affect
consumables material. The mechanical strength, dimensions and shape of the
consumable will change.
Use consumables that are suitable for the selected temperature range or
selected procedure.
The height sensor of the Eppendorf ThermoMixer FP automatically differentiates
between deepwell plates and microplates.
When inserting microplates, make sure that the height sensor is not covered.
Take care that the height sensor does not get contaminated.
Inserting the plate
Insert the plate with the back edge first. Then press it down at the front.
Inserting tubes
Insert the tubes completely into the bores of the thermoblock.
Only Eppendorf ThermoMixer FP:
To ensure uniform temperature control,
place the lid on the thermoblock.
1 Height sensor
1
Operation
Eppendorf ThermoMixer
®
F0.5/F1.5/F2.0/FP
English (EN)
5.3 Installing the ThermoTop
The condens.protect technology available with ThermoTop prevents the formation of
condensation on the inner wall or the lid of the tube.
Prerequisites
• Tubes or plates have been inserted.
Place the ThermoTop on the device
vertically from above. The centering pins
behind the heating plate fit into the
recesses of the ThermoTop.
• The ThermoTop is correctly positioned if
the seal is fully flush with the upper part
of the device.
• The blue LED of the ThermoTop lights.
• The symbol appears in the display.
Functioning principle of the ThermoTop
• In order to prevent the formation of any condensate in a reliable manner, the
device first heats the ThermoTop until it reaches the set temperature. The
tempering of the thermoblock occurs with a delay.
• The temperature sensor of the thermoblock reacts to the temperature of
samples: after inserting samples into a pre-heated thermoblock, the displayed
actual temperature may fall temporarily.
• While the device is tempering, the blue LED of the ThermoTop is flashing.
19
Operation
20
Eppendorf ThermoMixer
®
F0.5/F1.5/F2.0/FP
English (EN)
5.4 Mixing
The mixing frequency can be adjusted in steps of 50 rpm.
• Eppendorf ThermoMixer F0.5: 300 rpm – 2 000 rpm
• Eppendorf ThermoMixer F1.5: 300 rpm – 1 500 rpm
• Eppendorf ThermoMixer F2.0: 300 rpm – 1 500 rpm
• Eppendorf ThermoMixer FP: 300 rpm – 2 000 rpm
5.4.1 Mixing without temperature control
1. In order to switch off the temperature control, press the temp off key.
2. Set the mixing frequency with the speed arrow keys.
3. In order to start the mixing process, press the start/stop key.
• The symbol flashes on the display.
4. In order to end the mixing process, press the start/stop key.
• The display shows the last used parameters.
5.4.2 Mixing and tempering
1. Set the temperature with the temp arrow keys.
The device immediately starts to perform the temperature control.
2. Set the mixing frequency with the speed arrow keys.
3. In order to start the mixing process, press the start/stop key.
• The symbol flashes on the display.
• The display shows the actual temperature/set temperature and the mixing
frequency.
4. In order to end the mixing process, press the start/stop key.
• The display shows the last used parameters.
• Temperature control is continued.
Operation
Eppendorf ThermoMixer
®
F0.5/F1.5/F2.0/FP
English (EN)
5.4.3 Short Mix
Use the Short Mix function for mixing for a short while without temperature control.
1. Set the mixing frequency with the speed arrow keys.
2. Keep the short key pressed.
The mixing process continues as long as the short key will be pressed.
3. In order to end Short Mix, release the short key.
5.5 Temperature control
NOTICE! Damage to electronic components due to condensation.
Condensate can form in the device after it has been moved from a cool
environment to a warmer environment.
After installing the device, wait at least for 3 h. Only then connect the device
to the mains.
The Eppendorf ThermoMixer F0.5/F1.5/F2.0/FP can be used for temperature control in a
range of 4 °C above the ambient temperature to 100 °C.
• When the actual temperature flashes on the display, the device is not in
temperature control mode operation.
• As soon as the set temperature is changed with the temp arrow keys, the
device begins to perform temperature control.
• When the set temperature has been reached, the display only shows one
value.
21
5.5.1 Temperature control without mixing process
1. To switch off the mixing function, use the speed arrow keys to select the 0 rpm setting
(▼ below 300 rpm or ▲ above 1 500 rpm or 2 000 rpm).
2. Using the temp arrow keys set the temperature.
• The device immediately starts to perform temperature control.
• When the set temperature is not changed, the actual temperature flashes on the
display and the device does not perform temperature control.
3. To manually start the temperature control procedure, press the start/stop key.
• The symbol flashes on the display.
• The display shows the actual temperature/set temperature.
Operation
22
Eppendorf ThermoMixer
®
F0.5/F1.5/F2.0/FP
English (EN)
5.6 Menu
5.6.1 Navigate the menu
To change settings, proceed as follows:
1. In order to open the menu, keep the menu/enter key pressed.
2. Select the menu item with the menu arrow key.
3. To confirm your selection, press the menu/enter key.
4. Change the settings with the menu arrow key.
5. To confirm the changed setting, press the menu/enter key.
A tick appears in front of the setting.
6. To exit the menu level, select Back in the menu and press the menu/enter key.
5.6.2 Menu structure
Menu items and
Description Symbol on
options
Key lock (Key lock )
• Key lock on • Parameters cannot be changed.
• Key lock off • Parameters can be changed.
Volume (Volume) The signal tone for error messages is always output at
medium volume level regardless of the speaker
settings.
• Set the volume of the speaker:
Volume 1, Volume 2, Volume 3
• Switching the speaker off:
Volume off
Contrast (Contrast) • Set the contrast:
0 %, 25 %, 50 %, 75 %, 100 %
Service (Service) • Set the service interval:
After 500 operating hours
After 1000 operating hours
After 2000 operating hours
No notification
Back: Go to next higher menu level.
the display
Operation
English (EN)
Eppendorf ThermoMixer
®
F0.5/F1.5/F2.0/FP
5.7 Loading saved parameters
The 37 °C to 95 °C keys can be used to quickly select a temperature for a temperature
control procedure for an unlimited period of time. Use the temp off key to switch off
temperature control.
Temperature Mixing frequency
Key 37 °C 37 °C off
Key 42 °C 42 °C off
Key 56 °C 56 °C off
Key 95 °C 95 °C off
Key temp off off off
To call a saved temperature, press a direct selection key (37 °C to 95 °C).
• The LED above the key lights blue.
• The display shows saved parameters.
To start temperature control, press the start/stop key.
To perform temperature control and mixing at the same time, also set the mixing
frequency using the speed arrow keys.
To exit the displayed parameters, set different values for the temperature or
mixing frequency.
23
Troubleshooting
24
Eppendorf ThermoMixer
English (EN)
®
F0.5/F1.5/F2.0/FP
6 Troubleshooting
If you cannot remedy an error with the recommended measures, please contact your local
Eppendorf partner. The contact address can be found online at:
www.eppendorf.com.
6.1 General errors
Symptom/
message
Display remains
dark.
Set temperature
is not reached.
ThermoTop LED
does not light.
ThermoTop does
not fit on the
device.
The device does
not mix nor
control the
temperature.
Cause Remedy
• No mains connection.
Set temperature is less than 4 °C
above ambient temperature.
• The interface between the
device and the ThermoTop is
dirty.
The lid is attached to the
thermoblock.
Various causes are possible.
6.2 Error messages
Symptom/
message
Error message
preceded by a
number code.
Cause Remedy
• Various causes are possible. 1. Switch off device and wait
Check the mains connection
and the power supply.
Switch the device on.
Set up the device in a cooler
environment.
Remove any dirt from the front
of the ThermoTop.
Remove any dirt from the top of
the device, especially from the
viewing window in front of the
thermoblock.
If using the ThermoTop, do not
use the lid.
Contact your local Eppendorf
partner.
10 seconds.
2. Switch on device.
If the error message appears
again, contact your local
Eppendorf partner.
Maintenance
®
Eppendorf ThermoMixer
F0.5/F1.5/F2.0/FP
English (EN)
7 Maintenance
7.1 Setting service intervals
The Eppendorf ThermoMixer F0.5/F1.5/F2.0/FP offers the option of activating a reminder
that the device needs to be serviced. To set a service interval, proceed as follows:
1. Under Menu > Settings > select the Service menu item. Confirm with the menu/enter
key.
2. Select a service interval with the menu arrow keys (after 500, 1 000 or 2 000 operating
hours).
To switch off the notification, select No notification.
When the specified operating hours have been reached, a message appears. Contact your
local Eppendorf partner. The contact addresses can be found online at
www.eppendorf.com/worldwide.
7.2 Cleaning
Clean the housing of the Eppendorf ThermoMixer F0.5/F1.5/F2.0/FP regularly.
DANGER! Electric shock as a result of penetration of liquid.
Switch off the device and disconnect the power plug before starting cleaning
or disinfection work.
Do not allow any liquids to penetrate the inside of the housing.
Use closed tubes and closed plates.
Do not spray clean/spray disinfect the housing.
Only plug the device back in if it is completely dry, both inside and outside.
25
NOTICE! Damage from the use of aggressive chemicals.
Do not use any aggressive chemicals on the device or its accessories, such as
strong and weak bases, strong acids, acetone, formaldehyde, halogenated
hydrocarbons or phenol.
If the device has been contaminated by aggressive chemicals, immediately
clean it by means of a mild cleaning agent.
NOTICE! Corrosion from aggressive cleaning agents and disinfectants.
Do not use corrosive cleaning agents, aggressive solvents or abrasive
polishes.
Do not use lab cleaners with sodium hypochlorite.
Maintenance
26
Eppendorf ThermoMixer
English (EN)
Auxiliary equipment
• Lint-free cloth
• Mild, soap-based lab cleaner
• Dist. water
Cleaning the Eppendorf ThermoMixer F0.5/F1.5/F2.0/FP
1. Switch off the Eppendorf ThermoMixer F0.5/F1.5/F2.0/FP and disconnect it from the
power supply.
2. Clean all of the outer parts of the Eppendorf ThermoMixer F0.5/F1.5/F2.0/FP with a
mild soap solution and a lint-free cloth.
3. Wipe off the soap solution with dist. water.
4. Dry all cleaned parts.
®
F0.5/F1.5/F2.0/FP
7.3 Disinfection/Decontamination
DANGER! Electric shock as a result of penetration of liquid.
Switch off the device and disconnect the power plug before starting cleaning
or disinfection work.
Do not allow any liquids to penetrate the inside of the housing.
Use closed tubes and closed plates.
Do not spray clean/spray disinfect the housing.
Only plug the device back in if it is completely dry, both inside and outside.
Auxiliary equipment
• Lint-free cloth
• Disinfectant
1. Switch the Eppendorf ThermoMixer F0.5/F1.5/F2.0/FP off and isolate from the power
supply.
2. Allow the device to cool down.
3. Clean the device (see Cleaning on p. 25).
4. Select a disinfection method which complies with the legal requirements and
regulations applicable to your range of application.
5. Wipe the surfaces with the lint-free cloth and disinfectant.
Maintenance
®
Eppendorf ThermoMixer
F0.5/F1.5/F2.0/FP
English (EN)
7.4 Decontamination before shipment
If you are shipping the device to the authorized Technical Service for repairs or to your
authorized dealer for disposal please note the following:
WARNING! Risk to health from contaminated device
1. Observe the notes on the decontamination certificate. You find it as a PDF file
on our website (
www.eppendorf.com/decontamination).
2. Decontaminate all the parts you would like to dispatch.
3. Include the fully completed decontamination certificate in the package.
7.5 Verification of temperature control
To verify the temperature accuracy of the thermoblock, use the Eppendorf Temperature
Verification System – Single Channel. In combination with the temperature sensor for the
Eppendorf ThermoMixer F0.5/F1.5/F2.0/FP the exact temperature in the thermoblock can
be measured.
Details on the verification process with the Eppendorf Temperature Verification System –
Single Channel can be found in the corresponding operating manual.
27
Transport, storage and disposal
28
Eppendorf ThermoMixer
English (EN)
®
F0.5/F1.5/F2.0/FP
8 Transport, storage and disposal
8.1 Transport
CAUTION! Risk of injury due to lifting and carrying heavy loads
The device is heavy. Lifting and carrying the device can lead to back injuries.
Transport and lift the device with an adequate number of helpers only.
Use a transport aid to transport the device.
Use the original packaging for transport.
Air temperature Relative humidity Atmospheric pressure
General transport -25 °C – 60 °C 10 % – 75 %30 kPa – 106 kPa
Air freight -40 °C – 55 °C 10 % – 75 %30 kPa – 106 kPa
8.2 Storage
Air temperature Relative humidity Atmospheric pressure
In transport packaging -25 °C – 55 °C 10 % – 95 %70 kPa – 106 kPa
Without transport
packaging
8.3 Disposal
In case the product is to be disposed of, the relevant legal regulations are to be observed.
Information on the disposal of electrical and electronic devices in the European
Community:
Within the European Community, the disposal of electrical devices is regulated by
national regulations based on EU Directive 2002/96/EC pertaining to waste electrical and
electronic equipment (WEEE).
According to these regulations, any devices supplied after August 13, 2005, in the
business-to-business sphere, to which this product is assigned, may no longer be
disposed of in municipal or domestic waste. To document this, they have been marked
with the following identification:
-5 °C – 45 °C 10 % – 95 %70 kPa – 106 kPa
Transport, storage and disposal
Eppendorf ThermoMixer
®
F0.5/F1.5/F2.0/FP
English (EN)
Because disposal regulations may differ from one country to another within the EU,
please contact your supplier if necessary.
In Germany, this is mandatory from March 23, 2006. From this date, the manufacturer has
to offer a suitable method of return for all devices supplied after August 13, 2005. For all
devices supplied before August 13, 2005, the last user is responsible for the correct
disposal.
29
Technical data
30
Eppendorf ThermoMixer
®
F0.5/F1.5/F2.0/FP
English (EN)
9 Technical data
9.1 Power supply
Power connection 100 V – 130 V ±10 %, 50 Hz – 60 Hz
220 V – 240 V ±10 %, 50 Hz – 60 Hz
Power consumption Maximum 200 W
Overvoltage category II
Degree of pollution 2
Protection class I
9.2 Weight/dimensions
Dimensions Width: 20.6 cm (8.1 in)
Depth: 30.4 cm (12.0 in)
Height: ThermoMixer F0.5: 16.3 cm (6.4 in)
ThermoMixer F1.5: 17.0 cm (6.7 in)
ThermoMixer F2.0: 17.0 cm (6.7 in)
ThermoMixer FP: 16.4 cm (6.5 in)
Weight ThermoMixer F0.5: 6.2 kg (13.7 lb)
ThermoMixer F1.5: 6.3 kg (13.9 lb)
ThermoMixer F2.0: 6.3 kg (13.9 lb)
ThermoMixer FP: 6.1 kg (13.4 lb)
9.3 Ambient conditions
Ambience Only for use indoors.
Ambient temperature 5 °C – 40 °C
Relative humidity 10 % – 90 %, non-condensing.
Atmospheric pressure 79.5 kPa – 106 kPa
Technical data
®
Eppendorf ThermoMixer
F0.5/F1.5/F2.0/FP
English (EN)
9.4 Application parameters
9.4.1 Temperature control
Temperature control range Minimum: 4 °C above ambient temperature
Maximum: 100 °C
Temperature setting 1 °C – 100 °C,
adjustable in steps of 1 °C
Temperature accuracy Set temperature
20 °C – 45 °C
Eppendorf ThermoMixer F0.5
Eppendorf ThermoMixer F1.5
Eppendorf ThermoMixer F2.0
Eppendorf ThermoMixer FP
Temperature homogeneity
in relation to all positions of the
±0.5 °C
±0.5 °C
±0.5 °C
±1.0 °C
Set temperature
20 °C – 45 °C
Set temperature
< 20 °C or > 45 °C
±0.5 °C
±0.5 °C
±0.5 °C
±4.0 °C
Set temperature
< 20 °C or > 45 °C
thermoblock
Eppendorf ThermoMixer F0.5/F1.5/F2.0/FP ±0.5 °C ±1.5 °C
Heating rate Eppendorf ThermoMixer F0.5: 15 °C/min
Eppendorf ThermoMixer F1.5: 11 °C/min
Eppendorf ThermoMixer F2.0: 13 °C/min
Eppendorf ThermoMixer FP: 18 °C/min
The change of temperature in filled tubes is
slower.
31
9.4.2 Mix
Mixing frequency can be set in increments of 50 rpm
Eppendorf ThermoMixer F0.5 300 rpm – 2 000 rpm
Eppendorf ThermoMixer F1.5 300 rpm – 1 500 rpm
Eppendorf ThermoMixer F2.0 300 rpm – 1 500 rpm
Eppendorf ThermoMixer FP 300 rpm – 2 000 rpm
9.5 Interface
USB interface
For Eppendorf service only.
Ordering information
32
Eppendorf ThermoMixer
®
F0.5/F1.5/F2.0/FP
English (EN)
10 Ordering information
CAUTION! Poor safety due to incorrect accessories and spare parts.
The use of accessories and spare parts other than those recommended by
Eppendorf may impair the safety, functioning and precision of the device.
Eppendorf cannot be held liable or accept any liability for damage resulting from
the use of incorrect or non-recommended accessories and spare parts, or from
the improper use of such equipment.
Only use accessories and original spare parts recommended by Eppendorf.
10.1 Device and accessories
Order no.
(International)
5386 000.010 – 220 V – 240 V
– 5386000028 100 V – 130 V
5384 000.012 – 220 V – 240 V
– 5384000020 100 V – 130 V
5387 000.013 – 220 V – 240 V
– 5387000021 100 V – 130 V
5385 000.016 – 220 V – 240 V
– 5385000024 100 V – 130 V
5308 000.003 5308000003 ThermoTop
5363 000.233 5363000233 Lid
Order no.
(North America)
Description
ThermoMixer F0.5
with thermoblock for 24 0.5 mL tubes
ThermoMixer F1.5
with thermoblock for 24 1.5 mL tubes
ThermoMixer F2.0
with thermoblock for 24 2.0 mL tubes
ThermoMixer FP
with thermoblock for microplates and
Deepwell plates
with condens.protect technology
for ThermoMixer F0.5/F1.5/F2.0/FP
Eppendorf ThermoMixer
10.2 Tubes and plates
Order no.
(International)
Order no.
(North America)
Description
Eppendorf Safe-Lock Tube 0.5 mL
500 pieces
0030 121.023 022363611 clear
Eppendorf Safe-Lock Tube 1.5 mL
1,000 pieces
0030 120.086 - clear
Eppendorf Safe-Lock Tube 2.0 mL
1,000 pieces
0030 120.094 - clear
Eppendorf Deepwell Plate 384/200 µL
40 plates, wells clear, white border color
0030 521.102 951031003 PCR Clean
Eppendorf Deepwell Plate 96/500 µL
40 plates, wells clear, white border color
0030 501.101 951031801 PCR Clean
Eppendorf Deepwell Plate 96/1000 µL
20 plates, wells clear, white border color
0030 501.209 951032603 PCR Clean
Eppendorf Deepwell Plate 96/2000 µL
20 plates, wells clear, white border color
0030 501.306 951033405 PCR Clean
Ordering information
®
F0.5/F1.5/F2.0/FP
English (EN)
33
All plates are available with different border colors (red, yellow, green and blue) and
purity qualities, in large packs as well as with barcoding on request. You can find further
information in our catalog or on our website
www.eppendorf.com.
10.3 Temperature Verification System
Order no.
(International)
Order no.
(North America)
Description
0056 000.003 0056000003 Temperature Verification System USB –
Single channel
For Mastercycler nexus, Mastercycler pro
und Mastercycler ep, ThermoMixer,
ThermoStat
Temperature sensor
for Temperature Verification System USB –
Single channel
0056 002.006 0056002006 384 Well
Index
34
Eppendorf ThermoMixer
English (EN)
®
F0.5/F1.5/F2.0/FP
Index
A
Actual temperature................................. 17
Application parameters .......................... 31
C
Cleaning ................................................. 26
condens.protect...................................... 19
D
Danger symbol
Device............................................... 14
Decontamination .................................... 27
Deepwell plate.................................... 6, 18
Display................................................ 8, 17
Disposal .................................................. 28
G
Glossary.................................................... 6
H
Hazards................................................... 11
Heating rate............................................ 31
I
Installation
Device............................................... 15
Selecting the location....................... 15
Intended use
Hazards............................................. 11
L
Lid .......................................................6, 18
M
Main illustration........................................7
Mains/power cord socket..........................8
Mains/power switch ..................................8
Menu .......................................................22
Menu arrow key ................................16
Navigation.........................................22
Overview ...........................................22
Microplate ...........................................6, 18
Mix
Application parameters.....................31
Mixing .....................................................20
Setting the mixing frequency............20
start ...................................................16
stop....................................................16
Symbol ..............................................17
without temperature control .............20
Mixing frequency....................................17
set......................................................16
O
Operating controls ..............................8, 16
P
PCR ...........................................................6
PCR plate.............................................6, 18
R
rpm............................................................6
K
Key lock
set ..................................................... 22
Symbol.............................................. 17
S
Safety precautions...................................11
Selecting the location..............................15
Set temperature ......................................17
Short Mix ..........................................16, 21
Speaker
Symbol.............................................. 17
Storage ................................................... 28
T
Technical data
Ambient conditions .......................... 30
Application parameters .................... 31
Power supply.................................... 30
Weights/dimensions ......................... 30
Temperature
set ..................................................... 16
Temperature control............................... 21
Application parameters .................... 31
Switching off the temperature
control .............................................. 20
Symbol.............................................. 17
Temperature key..................................... 16
Thermoblock ............................................ 8
ThermoTop......................................... 6, 19
Symbol.............................................. 17
Tubes...................................................... 18
Eppendorf ThermoMixer
®
F0.5/F1.5/F2.0/FP
Index
English (EN)
35
U
USB interface ........................................... 8
V
Verification of temperature control........ 27
Volume
set ..................................................... 22
W
Well........................................................... 6
Index
36
Eppendorf ThermoMixer
English (EN)
®
F0.5/F1.5/F2.0/FP
Certificates
Evaluate your manual
Give us your feedback.
www.eppendorf.com/manualfeedback
Your local distributor: www.eppendorf.com/contact
Eppendorf AG · 22331 Hamburg · Germany
eppendorf@eppendorf.com · www.eppendorf.com
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