Eppendorf Thermoblock slides User Manual
Wechselblock slides
Exchangeable Thermoblock slides
Bedienungsanleitung
Operating instructions
Inhalt / Table of contents
Bedienungsanleitung . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Operating instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Nachdruck und Vervielfältigung – auch auszugsweise – nur mit Genehmigung.
No part of this publication may be reproduced without the prior permission of the
copyright owner.
Copyright
©
2005 Eppendorf AG, Hamburg
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Table of contents
1 General . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
2 Safety instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
3 Mounting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
4 Application . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
5 Cleaning / Service . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
5.1 Replacing the snap seal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
5.2 Replacing the lid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
6 Technical data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
7 Ordering information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
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1 General / 2 Safety instructions
1 General
Only experienced laboratory personnel are permitted to use the slides thermoblock. The exchangeable Thermoblock "slides" (object carriers) is designed for
processing microarrays composed of either nucleic acids or proteins. The slides
thermoblock is used to process microarrays; these can be based on nucleic
acids as well as proteins. The slides thermoblock is only intended to be used
with the Thermomixer comfort / R and ThermoStat plus basic devices. See their
respective operating manuals for more information. The slides thermoblock is
designed for rotary speeds up to 1400 rpm and a temperature of up to 99 °C.
2 Safety instructions
– When exchanging a heated block, please keep in mind that contact with the
surface of the opened exchangeable Thermoblock, its underside or the
thermal base on the basic device can result in burns!
– When changing a hot block, do not touch the surface or underside of the
opened block or the thermobase on the basic device!
– Always tighten screws finger-tight when changing a block.
– Do not use the thermoblock if it is visibly damaged.
– Before using the mix function, the lid of the slides thermoblock must be firmly
shut (lid parallel to block).
– If the block is not identified automatically by older Thermomixer comfort / R
basic devices, it is vital that you observe the maximum rotation speed of
1400 rpm (the rotation speed is not automatically limited in this case).
Please contact our Customer Service department (see page 22) to update the
software in your basic device.
– This operating manual must also be handed over if you give the slides
thermoblock to someone else.
– Make certain that the slides are inserted precisely into the recesses.
– The outer 4 mm of both face sides of the standard slides are required for fixing
in the device through the lid. This means that they are not available as a
reaction area. The combined height of the slide and the reaction area shall not
exceed 5 mm.
– Please use desalted water when positioning a damp chamber in the block.
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3 Mounting / 4 Application
3 Mounting
To mount the exchangeable Thermoblock "slides", open the lid by
simultaneously pressing the two pressure points on the front edge of the lid. The
pressure points are labeled with "push". Adjust the exchangeable Thermoblock
on the basic device in such a way that the label can be seen from the front.
Screw it tight using an Allen key.
After loading the exchangeable Thermoblock with up to 4 slides, and prior to
temperature control / rotation, the lid must be closed by simultaneously pressing
the two pressure points. It is absolutely necessary to ascertain that the lid has
been correctly closed prior to starting the device. The slides are only fixed when
the lid is closed, and only then rotation can begin.
After placing the slides thermoblock on Thermomixer comfort / R basic devices,
the block is identified. This automatically limits the maximum rotation speed to
1400 rpm. If the block is not identified automatically by older Thermomixer
comfort basic devices, it is vital that you observe the maximum rotation speed of
1400 rpm. Please contact our Customer Service department (see page 22) to
update the software in your basic device.
4 Application
Four slide positions are provided on the exchangeable Thermoblock. A reaction
area must be positioned above the microarray on the slide surface in order to
carry out the reaction. Commercially available hybridization chambers or cover
glass of various sizes are currently used to create the reaction chamber. When
using hybridization chambers or cover glass it is important to consider that 4 mm
of the front sides of a standard slide are not available as reaction area due to the
fixing by the lid of the exchangeable Thermoblock. The total height of the slide
and the hybridization chamber shall also not exceed 5 mm. Also it is important
that the hybridization chamber doesn’t overlap the edges of the slide.
The hybridization chamber or the cover glass must be fixed on the slide surface
and sealed as air-tight as possible, thus preventing the leakage of reaction
solution during temperature control and / or rotation.
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