Eppendorf Thermoblock for slides DC User Manual

Exchangeable Thermoblock

for slides DC

Operating instructions

Table of contents

1 General . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3

2 Safety instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3

3 Mounting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4

4 Application . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

5 Cleaning / Service . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

5.1 Replacing the snap seal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

5.2 Replacing the lid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

6Technical data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

7Ordering information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

No part of this publication may be reproduced without the prior permission of the
copyright owner.

Copyright

©

2005 Eppendorf AG, Hamburg

2

1 General / 2 Safety instructions

1 General

Only experienced laboratory personnel are permitted to use the slides thermo­block. The exchangeable Thermoblock for slides DC is especially designed for
processing either DNA or protein microarrays using the Eppendorf hybridization
frames in research laboratories in the fields of bioscience, medicine and
chemistry. The Thermoblock for slides DC can be used on both the Thermomixer
comfort or the ThermoStat plus. It can be used for rotational movement up to a
maximum of 1,400 rpm and at a maximum temperature of 70 °C.

2 Safety instructions

– When exchanging a heated block, please keep in mind that contact with the

surface of the opened exchangeable thermoblock, its underside or the thermal
base on the basic device can result in burns! Exchange the thermoblock once
it has cooled down.

– Make certain that the slides are inserted precisely into the recesses. The

Thermoblock for slides DC is compatible with slides with a maximum length of

76.70 mm.
– Make sure that the block is clean (no glass particles) before placing slides.
– Make sure that the pressure plate is correctly fixed. See mounting part of this

manual.

– The lid of the Thermoblock for slides DC must be tightly closed (lid parallel to

block) prior to using the mix function.

– Be sure to observe the maximum rotational speed of 1,400 rpm and the

maximum temperature setting of 70 °C. The ThermoStat plus basic device has
no block recognition function. There is no automatic limitation of the

temperature control.
– Do not use damaged thermoblocks.
– Exchangeable thermoblocks may only be used in conjunction with the

ThermoStat plus and Thermomixer comfort devices. See their respective

operating manuals for more information.
– This operating manual must also be handed over if you give the slides

thermoblock to someone else.

3

3 Mounting

1. To mount the exchangeable Thermoblock for slides DC, open the lid by

simultaneously pressing the two pressure points on the fr ont edge of the lid.
The pressure points are labeled with "push".

Figure 1: Drawing of the
Thermoblock for slides DC

1 Pressure plate
2 Screws

3

3 Lid seal
4 Label

2
1

4

2. Alternately unscrew the 2 screws of the pressure plate until the pressure is

completely released (Figure 1).

3. Adjust the exchangeable Thermoblock for slides DC on the basic device in

such a way that the label can be seen from the front. Screw it tight using a
Hexagon key.

4

3 Mounting

4. Place the slides (up to 4) in the Thermoblock for slides DC. The slides have

to be inserted with the sticker facing the front part of the thermoblock
(Figure 2).

Figure 2: Drawing of the opened
Thermoblock for slides DC

1 Slide
2 Pressure plate location ridges
3 Holes to remove the slide
4 Front part of the thermoblock

2
1

3

4

2

5

s

3 Mounting

5. Position the pressure plate on top of the Thermoblock for slides DC and

push it firmly into the location ridges.
Hold both plate and thermoblock tightly and alternately fasten both screws

completely using the screwing tool (Figure 3).

Screwing tool

Pressure plate
location ridge

Pressure plate
location ridge

Figure 3: Schematic drawing of the Thermoblock for slides DC assembly and the

crewing tool.

6

Pressure plate

Thermoblock
for slides DC

assembly

3 Mounting / 4 Application

6. Close the lid and push down the 2 pressure points on the front edges of the

lid. It is absolutely necessary to ascertain that the lid has been correctly
closed prior to starting the device.

In the case of the Thermomixer comfort, block identification occurs after the
exchangeable Thermoblock for slides DC has been correctly positioned.
This results in an automatic limitation of maximum possible rotational speed
(1400 rpm) and temperature setting (70 °C).

In the case of the ThermoStat plus there is no block identification function
for technical reasons. Please set for max. temperature of 70 °C.

7. After the incubation step, open the Thermoblock for slides DC lid and

unscrew the pressure plate (caution: hot plate) by alternating between both
screws.

4 Application

An Eppendorf hybridization frame must be positioned above the microarray on
the slide surface in order to carry out the reaction.
In the case of using DualChip™ microarrays from Eppendorf, hybridization
frames are already placed on the slides.
Four slide positions are provided on the exchangeable Thermoblock for slides
DC.

7

5 Cleaning / Service

–To clean the Thermoblock for slides DC, switch off the power supply and

unplug the device.
– Ascertain that the surface of the opened Thermoblock for slides DC and its

underside as well as the thermal base on the basic device have cooled down

completely to minimize the risk of burns.
– Unscrew the block from the basic device.
– Clean the exchangeable Thermoblock for slides DC immediately if sample

liquid finds its way into the bores. A mild soap solution or lab cleaner can be

used for cleaning. The device should be protected from organic solutions and

aggressive chemicals. For disinfection purposes, please use alcohol-based

infectants.
– The sealant material in the lid can be removed and cleaned or replaced.

5.1 Replacing the snap seal

If sample liquid finds its way into the two snap seals of the block, remove them
from the block and wash them separately in a soap solution or in an ultrasonic
bath or replace them.
The removal of the locking devices from the exchangeable Thermoblock for
slides DC is carried out by pressing both of the side catches beneath the block
together and simultaneously pressing them out of the block (see Figure 4). Be
aware of the direction when reinserting the locking systems into the block: the
directional marking on the right side must point forward while that on the left side
points to the rear.

Figure 4: Changing the snap seal

1. Press the side catches together
simultaneously

2. Push out the lock

3 Directional marking

2.

3

1.

8

5 Cleaning / Service

5.2 Replacing the lid

The lid, including the seal, can also be replaced when damaged: To do this,
loosen and remove the 2 slotted head screws at the side of the lid with a slotted
head screwdriver (N° 2). Replace the lid and refasten it by retightening the 2
slotted head screws (Figure 5).

Figure 5: Replacing the lid

9

6 Technical data

Slide positions: max. of 4 positions
Slide dimensions: Length 74.5 – 76.7 mm

Width 24.7 – 26.3 mm

Height 0.9 – 1.2 mm
Rotational speed: max. 1400 rpm
Temperature control: to a maximum of 70 °C possible

– T

Temperature homogeneity: T

T

max
max

≤ 2 K * (at 70 °C)

min

– T

≤ 0,5 K * (at 45 °C)

min

Heating-up speed of the thermoblock: > 5 K / min *
Chemical resistance: The use of strong acids, bases or

acetone is not recommended.

This could result in damage

to the surface.
Cooling-off period from 70 °C to 10 K above the ambient temperature: 20 min

Data determined with a Thermomixer comfort.

*

10

7 Ordering information

Product Description Order no. for

Thermoblock for slides DC 022670786
Lid with seal

Snap seal

Products

Thermomixer comfort / R
115 V
220 V
ThermosStat plus (without block)
Silverquant

®

adapter

North America

0022670727
0022670743

022670107
022670158
022670204
956015073

Order no.
International

5368 000.100
5368 851.002

5368 852.009

5355 000.011

5352 000.010
5368 000.037

11

Eppendorf Offices

ASEAN

Eppendorf AG
Regional Office in Malaysia
Tel. [phone]
Fax [phone]
E-Mail: [e-mail]
Internet: www.eppendorf.com.my

AUSTRALIA / NEW ZEALAND

Eppendorf South Pacific Pty. Ltd.
Tel. [phone]
Fax [phone]
E-mail: [e-mail]
Internet: www.eppendorf.com.au

AUSTRIA

Eppendorf AG
c/o Schott Austria
Tel. [phone]
Fax [phone]
E-Mail: [e-mail]
Internet: www.eppendorf.com

BRAZIL

Eppendorf do Brasil Ltda.
Tel. [phone]
Fax [phone]
E-Mail: [e-mail]
Internet: www.eppendorf.com.br

CANADA

Brinkmann Instruments, Ltd.
Tel. [phone]
Fax [phone]
E-Mail: [e-mail]
Internet: www.brinkmann.com

CHINA

Eppendorf AG
Tel. [phone]
Fax [phone]
E-Mail: [e-mail]
Internet: www.eppendorf.cn

FRANCE

EPPENDORF FRANCE S.A.R.L.
Tel. [phone]
Fax [phone]
E-Mail: [e-mail]
Internet: www.eppendorf.fr

GERMANY

Eppendorf Vertrieb
Deutschland GmbH
Tel. [phone]
Fax [phone]
E-Mail: [e-mail]
Internet: www.eppendorf.de

INDIA

Eppendorf India Limited
Tel. [phone]
Fax [phone]
E-Mail: [e-mail]
Internet: www.eppendorf.co.in

ITALY

Eppendorf s.r.l.
Tel. [phone]
Fax [phone]
E-Mail: [e-mail]
Internet: www.eppendorf.it

Eppendorf Offices

JAPAN

Eppendorf Japan Co. Ltd.
Tel. [phone]
Fax [phone]
E-Mail: [e-mail]
Internet: www.eppendorf.jp

NORDIC

Eppendorf Nordic Aps
Tel. [phone]
Fax [phone]
E-Mail: [e-mail]
Internet: www.eppendorf.dk

SPAIN

Eppendorf Ibérica S.L.
Tel. [phone]
Fax [phone]
E-Mail: [e-mail]
Internet: www.eppendorf.es

SWITZERLAND

Vaudaux-Eppendorf AG
Tel. [phone]
Fax [phone]
E-Mail: [e-mail]
Internet: www.eppendorf.com

UNITED KINGDOM

Eppendorf UK Limited
Tel. [phone]
Fax [phone]
E-Mail: [e-mail]
Internet: www.eppendorf.co.uk

USA

Eppendorf North America
Tel. [phone]
Fax [phone]
E-Mail: [e-mail]
Internet: www.eppendorfna.com

OTHER COUNTRIES

see:
www.eppendorf.com/worldwide

is a registered trademark.

®

In touch with life

Your local distributor: www.eppendorf.com/worldwide

Eppendorf AG · 22331 Hamburg · Germany · Tel. [phone] · Fax [phone]

E-Mail: [e-mail]

Eppendorf North America, Inc. · One Cantiague Road, P.O. Box 1019

Westbury, N.Y. 11590-0207 USA · Tel. [phone] · Free phone [phone]

Fax [phone] · E-Mail: [e-mail]

Application Support

Europe, International: Tel. [phone] · E-Mail: [e-mail]

North America: Tel. [phone] ext. 2258 · E-Mail: [e-mail]

Asia, Pacific: Tel. [phone] · E-Mail: [e-mail]

eppendorf

B 5368 900.100-01/0805 · Printed in Germany