Eppendorf Temperature Verification System User Manual
Operating manual
Draft
Temperature Verification System USB Singlechannel
see
on p.Fig.Tab.p
.
Operating manual
0056000402
Temperature Verification System USB
Single-channel
Operating manual
Operating manual
Copyright© 2012 Eppendorf AG, Hamburg. No part of this publication may be reproduced
without the prior permission of the copyright owner.
eppendorf® Mastercycler® and vapoprotect® are registered trademarks of Eppendorf AG,
Hamburg, Germany.
Trademarks are not marked in all cases with ™ or ® in this manual.
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Temperature Verification System USB – Single-channel — Operating manual
Table of contents
1 Table of contents
1 Operating instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.1 Using this manual. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.2 Danger symbols and danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.2.1 Danger symbols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.2.2 Danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.3 Symbols used. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
1.4 Abbreviations used. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
1.5 Glossary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
2 Product description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
2.1 Delivery package . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
2.1.1 Temperature Verification System USB – Single-channel . . . . . . . . . . . . . . . . . 7
2.1.2 Temperature sensor 96 Well. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
2.1.3 Temperature sensor 384 Well. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
2.1.4 Temperature sensor 1,5 mL . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
2.2 Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
2.2.1 Temperature Verification System USB – Single-channel . . . . . . . . . . . . . . . . . 7
2.2.2 Design of the temperature sensor. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
2.2.3 Temperature sensor 96 Well. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
2.2.4 Temperature sensor 384 Well. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
2.2.5 Temperature sensor 1,5 mL . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
2.3 Certificate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
3 Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
3.1 Intended use. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
3.2 User profile . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
3.3 Information on product liability . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
3.4 Warnings for intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
3.5 Warnings for unintended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
4 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
4.1 Initial charging of the battery . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
4.2 Connecting the temperature sensor to the Measuring device . . . . . . . . . . . . . . . . . . 13
4.3 Connecting the Mastercycler nexus . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
4.4 Connecting the Mastercycler pro . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
4.5 Connecting the Mastercycler ep. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
4.6 Connecting the Mastercycler pro 384 and Mastercycler ep 384. . . . . . . . . . . . . . . . . 16
4.6.1 Connecting the Mastercycler pro 384 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
4.6.2 Connecting the Mastercycler ep 384 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
4.6.3 Preparing the Thermoblock 384 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
4.6.4 Removing oil . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
4.7 Connecting the Mastercycler to a computer and CycleManager pro/pro
XL
. . . . . . . . 18
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Temperature Verification System USB – Single-channel — Operating manual
4.8 Installing the Thermomixer and ThermoStat . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
4.8.1 Preparing the Thermomixer comfort and ThermoStat plus. . . . . . . . . . . . . . . 18
4.8.2 Connecting the Thermomixer and ThermoStat. . . . . . . . . . . . . . . . . . . . . . . . 18
5 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
5.1 Verifying the Mastercycler nexus, pro and ep. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
5.1.1 Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
5.1.2 Verifying the Mastercycler nexus and Mastercycler pro . . . . . . . . . . . . . . . . . 20
5.1.3 Verifying the Mastercycler ep . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
5.1.4 Verifying the Mastercycler pro/ep using the CycleManager pro . . . . . . . . . . . 23
5.2 Adjusting the Mastercycler nexus/pro/ep . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
5.2.1 Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
5.2.2 Adjusting the Mastercycler nexus and Mastercycler pro . . . . . . . . . . . . . . . . . 25
5.2.3 Adjusting the Mastercycler ep. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
5.2.4 Adjusting the Mastercycler pro/ep using the CycleManager pro. . . . . . . . . . . 28
5.3 Verifying the Thermomixer and ThermoStat. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
6 Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
6.1 General errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
6.1.1 Troubleshooting for the Measuring device . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
6.1.2 Troubleshooting for the Mastercyclers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
7 Transport, storage and disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
7.1 Packing. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
7.2 Disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
8 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
8.1 Servicing the Measuring device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
8.2 Servicing the Temperature sensor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
8.3 Removing oil from the thermoblock . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
8.4 Disinfection/Decontamination. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
8.5 Decontamination before shipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
9 Technical data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
9.1 Temperature Verification System USB – Single-channel . . . . . . . . . . . . . . . . . . . . . . 34
9.2 Mastercycler, Thermomixer and ThermoStat . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
9.3 Maximum permissible errors and accuracy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
9.3.1 Temperature Verification System USB – Single-channel . . . . . . . . . . . . . . . . 34
9.3.2 Mastercycler . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
9.3.3 Thermomixer and ThermoStat . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
9.3.4 ThermoMixer C, ThermoMixer F1.5, ThermoMixer FP and ThermoStat C. . . 35
10 Ordering information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
Index. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
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Temperature Verification System USB – Single-channel — Operating manual
1 Operating instructions
1 Operating instructions
1.1 Using this manual
Read this operating manual completely before using the device for the first time. Please also
note the operating instructions for the accessories, if applicable.
This operating manual is part of the product. Please keep it in a place that is easily
accessible.
Enclose this operating manual when transferring the device to third parties.
If this manual is lost, please request another one. For the current version, please refer to our
webpage www.eppendorf.com/worldwide
America).
1.2 Danger symbols and danger levels
The safety instructions in this manual appear with the following danger symbols and danger
levels:
1.2.1 Danger symbols
Biohazard Explosion
Electric shock Hot surface
(international) or www.eppendorfna.com (North
Hazard point Material damage
1.2.2 Danger levels
DANGER Will lead to serious injuries or death.
WARNING May lead to serious injuries or death.
CAUTION May lead to light to moderate injuries.
NOTICE May lead to material damage.
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Temperature Verification System USB – Single-channel — Operating manual
1.3 Symbols used
Symbol Meaning
Handling
1.
2.
Actions in the specified order
• List
Text Name of fields in the software
Useful information
1.4 Abbreviations used
PDF Portable document format
USB Universal serial bus
1.5 Glossary
A
Adjustment Tuning or alignment of a device to prevent systematic deviations to the extent
required for the observance of specifications. An adjustment requires an
intervention that permanently modifies the device.
T
Temperature
accuracy
Temperature
homogeneity
Verification Objective proof of the observance of defined specifications.
Deviation of the measured temperature from the specified temperature at a
given position on the thermoblock.
Maximum temperature difference between two positions of the thermoblock.
V
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Temperature Verification System USB – Single-channel — Operating manual
2 Product description
2 Product description
2.1 Delivery package
2.1.1 Temperature Verification System USB – Single-channel
For Mastercycler nexus, Mastercycler pro, Mastercycler ep, Thermomixer and ThermoStat
1× measuring device
1× plug head line (connecting cable between measuring device and temperature sensor)
1× mains/power supply device
4× international plug adapter
1× operating manual
1× device case
2.1.2 Temperature sensor 96 Well
1× Temperature sensor 96 Well
1× certificate
2.1.3 Temperature sensor 384 Well
1× Temperature sensor 384 Well
1× silicone mat for the Temperature sensor 384 Well
4× spacers
1× bottle with parrafin oil (25 mL)
1× certificate
2.1.4 Temperature sensor 1,5 mL
1× Temperature sensor 1,5 mL
1× certificate
2.2 Features
2.2.1 Temperature Verification System USB – Single-channel
Hint!
• The term "validation" has been replaced by "verification" in these
instructions. The term "validation" is still used in older software versions.
• The term "calibration" has been replaced by "adjustment" in these
instructions. The term "calibration" is still used in older software versions.
The Temperature Verification System USB – Single-channel enables simple and fast inspection
of the block temperature of the following Mastercycler products:
• Mastercycler nexus
• Mastercycler pro
• Mastercycler ep
The Temperature Verification System USB – Single-channel requires a minimum software
version in the control panel of the thermal cycler.
This table contains the minimum software versions required to use the Temperature Verification
System USB – Single-channel. If the device or CycleManager pro software has an older version,
install the software version listed below.
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Temperature Verification System USB – Single-channel — Operating manual
Device Software version
Mastercycler nexus 1.4.0.0
Mastercycler pro 1.023
Mastercycler ep 4.605
CycleManager pro/pro
The Mastercyclers listed feature a precise system which meets all of the laboratory requirements
for documentation and the obligation to provide supporting documentation.
Furthermore, the block temperature of the Eppendorf Thermomixer and ThermoStat devices can
be measured using the Temperature sensor 1,5 mL and Temperature sensor 384 Well.
The Temperature Verification System USB – Single-channel consists of a digital display device
with connected temperature sensor. Various temperature sensors, which are geometrically
adapted to the various thermoblock bores, are available.
Verification and adjustment is based on a 2-point measurement with the Temperature Verification
System USB – Single-channel at 95 °C and 35 °C. The verification compares the difference
between the block temperature and the measured value using limit values. If the limit values are
exceeded, the adjustment uses the measured values from the Temperature Verification System
USB – Single-channel to set the thermoregulation of the tested device.
The user will be guided through the program via the instructions and the display of sensor
positions on the display. The measurements and calculations are performed automatically.
The verification and adjustment can be optionally output a print-out, which contains the entire
measuring procedure and results, and can be documented. Depending on the device type, the
data can be exported to a MultiMediaCard (MMC) or a USB storage medium.
2.2.2 Design of the temperature sensor
The temperature sensor consists of a grip and a temperature probe.
The grip contains the galvanic separation, adjustment data, and all of the data for the
temperature measurement.
2.2.3 Temperature sensor 96 Well
The Temperature sensor 96 Well and Temperature Verification System USB – Single-channel are
used to verify and adjust the following devices:
XL
2.5/2.6
• Mastercycler nexus (all device variant except Mastercycler nexus flat)
• Mastercycler pro, Mastercycler pro S
• Mastercycler ep gradient, Mastercycler ep gradient S
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Temperature Verification System USB – Single-channel — Operating manual
2.2.4 Temperature sensor 384 Well
The Temperature sensor 384 Well and Temperature Verification System USB – Single-channel
are used to verify the following devices:
• Mastercycler pro 384
• Mastercycler ep 384
• ThermoMixer C
• ThermoMixer F1.5
• ThermoMixer FP
• ThermoStat C
The Mastercycler pro 384 is also adjusted using this sensor.
2.2.5 Temperature sensor 1,5 mL
The Temperature sensor 1,5 mL and Temperature Verification System USB – Single-channel are
used to verify the following devices:
• Thermomixer comfort
• Thermomixer compact
• ThermoStat plus
2.3 Certificate
The accuracy of the temperature sensor is confirmed by the accompanying certificate. This
ensures a traceability to national and international standards according to MRA (mutual
recognition arrangement from October 14, 1999, Paris, /http:www.bipm.org/pdf/mra.pdf).
The country-specific laws and quality assurance standards must be observed.
Each temperature sensor is verified after the adjustment and receives its own certificate with
information on validity.
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Temperature Verification System USB – Single-channel — Operating manual
3 Safety
3Safety
3.1 Intended use
The Temperature Verification System USB – Single-channel is a single-channel temperature
measuring device for verification and adjustment of thermoblock temperature control and
recording the temperature data of thermal cyclers. It supports the verification and adjustment of
the following Eppendorf product families:
• Mastercycler nexus
• Mastercycler pro
• Mastercycler ep
• Thermomixer
• ThermoStat
The Mastercycler gradient and Mastercycler ep realplex can no longer be verified and adjusted
with the Temperature Verification System USB – Single-channel.
The Temperature Verification System USB – Single-channel may only be used indoors.
3.2 User profile
The device and accessories may only be operated by trained and skilled personnel.
Before using the device, read the operating manual carefully and familiarize yourself with the
device's mode of operation.
3.3 Information on product liability
In the following cases, the designated protection of the device may be compromised. Liability for
any resulting property damage or personal injury is then transferred to the operator:
• The device is not used in accordance with the operating manual.
• The device is used outside of its intended use.
• The device is used with accessories or consumables which are not recommended by
Eppendorf.
• The device is maintained or repaired by people not authorized by Eppendorf.
• The user makes unauthorized changes to the device.
3.4 Warnings for intended use
Read the operating manual and observe the following safety instructions before using the
Temperature Verification System USB – Single-channel.
DANGER!
WARNING!
10
DANGER! Risk of explosion.
Do not operate the device in areas where work is completed with
explosive substances.
WARNING! Risk from incorrect supply voltage
Only connect the device to voltage sources which correspond to the
electrical requirements on the name plate.
Temperature Verification System USB – Single-channel — Operating manual
WARNING!
CAUTION!
CAUTION!
CAUTION!
WARNING! Infection by contaminated material.
There may be contaminated material on the device and accessories.
Work may only be completed on decontaminated devices.
Find out more about contamination risks before beginning work.
Check the device decontamination certificate.
Wear personal protective equipment (protective gloves, protective
goggles).
CAUTION! Risk of burns on thermoblock, heated lid and temperature
sensor.
The thermoblock, heated lid and temperature sensor can reach temperatures
of more than 95 °C.
During gradient operation, the temperature of the heated lid and thermoblock
is higher on the right side than on the left.
Use extreme caution when conducting measurements on the heated
thermoblock.
CAUTION! Burns on the silicone mat.
The heated lid heats the silicone mat to temperatures around 100 °C.
Open the heated lid without touching the silicone mat.
Wait until the temperature of the heated lid is below 30 °C.
CAUTION! Risk of burning due to flammable cleaning substances.
A small amount of cleaning substance remains on the thermoblock after the
cleaning procedure has been completed.
Cleaning substances such as ethanol or solvents may ignite when the
thermoblock heats up.
Open the heated lid.
Allow the cleaning substance to fully evaporate.
WARNING!
WARNING! Burns from hot surfaces.
The metal surfaces of the exchangeable thermoblock and the heating plate
reach temperatures up to 100 °C.
Do not touch hot surfaces.
Wait until the thermomixer or thermostat have cooled to room
temperature.
Test, service or repairs may only be conducted after the devices have
cooled to room temperature.
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Temperature Verification System USB – Single-channel — Operating manual
NOTICE!
NOTICE! Material damage from incorrect connections.
Only electrical connections may be made to devices described in the
operating manual.
Other connections are permitted only following consultation and
agreement with Eppendorf AG.
Only connect devices that meet the safety requirements defined in
IEC 60950-1.
3.5 Warnings for unintended use
CAUTION!
CAUTION! Poor safety due to incorrect accessories and spare parts.
The use of accessories and spare parts other than those recommended by
Eppendorf may impair the safety, functioning and precision of the device.
Eppendorf cannot be held liable or accept any liability for damage resulting
from the use of incorrect or non-recommended accessories and spare parts,
or from the improper use of such equipment.
Only use accessories and original spare parts recommended by
Eppendorf.
NOTICE!
NOTICE! Damage as a result of incorrect packing.
Eppendorf AG is not liable for damage caused by improper packing.
The device may only be stored and transported in its original packaging.
NOTICE!
NOTICE! Damage to the sensor and thermal cycler due to improper use.
Do not insert the sensor into thermal cyclers that are not compatible with
the Temperature Verification System USB – Single-channel.
When inserting the sensor or closing the heated lid, never use an
operational force significantly higher than the force recommended by the
thermal cycler manufacturer for handling a PCR plate.
Eppendorf accepts no warranty or liability for damage caused by incorrect
use of the Temperature Verification System USB – Single-channel.
12
NOTICE!
NOTICE! Damage to the sensor/cable connection as a result of the
cable being pulled.
Repeatedly pulling on the cable to remove the temperature sensor from the
thermoblock will cause damage to the sensor/cable connection.
Pull the thermosensor out of the thermoblock via the thermosensor
housing.
Do not apply tractive forces to the cable.
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