Eppendorf Temperature sensor for the 384well block User Manual

Temperatursensor für 384er Block

Temperature sensor for the 384well block

Bedienungsanleitung · Operating Manual

Inhalt / Contents

Bedienungsanleitung. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3

Operating Manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

Inhalt / Contents

Nachdruck und Vervielfältigung – auch auszugsweise – nur mit Genehmigung. No part of this publication may be reproduced without the prior permission of the copyright owner.

eppendorf Hamburg, Germany.

, Mastercycler® and vapoprotect® are registered trademarks of Eppendorf AG,

2008 Eppendorf AG, Hamburg

1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16

1.1 How to use this manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16

2 Safety precautions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16

3 Product information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17

3.1 Delivery package . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17

3.2 Setup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17

3.3 Accuracy of the measuring device and the measurements . . . . . . . . . . . . . . . . . . . . . 17

4 Operation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18

4.1 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18

4.1.1 Filling the measurement positions with oil . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18

4.1.2 Inserting of the spacers and inserting, repositioning and removing

the temperature sensor. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18

4.1.3 Cleaning the measurement positions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

4.2 Validation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

4.2.1 Validation of the Mastercycler 384 with software version 2.22 . . . . . . . . . . . . . . . . . . 21

4.2.2 Validation of the Mastercycler 384 with software version 2.3 and above . . . . . . . . . . 23

4.2.3 Validation of the Mastercycler

with Control Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23

4.2.4 Validation of the Mastercycler pro and Mastercycler ep 384

using the CycleManager pro / proXL or ep CycleManager . . . . . . . . . . . . . . . . . . . . . 23

4.3 Calibration / adjustment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23

4.3.1 Calibration / adjustment of the Mastercycler 384

with software version 2.30 and above . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23

4.3.2 Calibration / adjustment of the Mastercycler pro 384 and Mastercycler ep 384 . . . . . 23

4.3.3 Calibration / adjustment of the Mastercycler pro and Mastercycler ep 384

using the CycleManager pro / proXL or ep CycleManager . . . . . . . . . . . . . . . . . . . . . . 24

5 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

6 Error messages. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

7 Disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

8 Technical data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26

9 Ordering information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26

pro 384 and Mastercycler ep 384

Contents

1 Introduction / 2 Safety precautions

1 Introduction

The 348-well block sensor and the temperature validation system enable validation and calibration / adjustment of the Mastercycler 384, Mastercycler pro 384 and

1/2

Mastercycler ep 384.

1.1 How to use this manual

This operating manual for the 384-well block temperature sensor is a supplement to the user manual for the temperature validation system. It is not a replacement.

Before using the 384-well block temperature sensor for the ﬁrst time, you should also read the operating manual for the temperature validation system. The current version of the operating manual is available on the internet at www.eppendorf.com

2 Safety precautions

Risk of burns

Introduction / Safety precautions

When inserting, repositioning and removing the temperature sensor and the silicone mat during and at the end of validation and calibration, take care not to touch the heated lid.

Caution must be used when removing or repositioning the temperature sensor during and at the end of validation or calibration. The silicone mat is subjected to extreme heat by the heated lid. Allow the silicone mat to cool down with the heated lid open to around 30 °C or below (approx. 1 – 2 min).

The sensor cable should not be kinked outside the jacketed area. Should the sensor cable be damaged, the temperature sensor should no longer be used.

After cleaning the measurement positions the Mastercycler 384, Mastercycler pro 384 or the Mastercycler ep 384 should not be switched back on immediately as otherwise traces of the cleaning agent might ignite. The Mastercycler 384, Mastercycler pro 384 or the Mastercycler ep 384 should be ventilated by being left switched off with the lid open for a period of time.

The safety precautions in the operating manual of the temperature validation system and the relevant device (Mastercycler 384, Mastercycler pro 384) must be observed without fail.

3 Product information

3.1 Delivery package

– Temperature sensor for the 384well block – Operating manual for the 384 temperature sensor (this manual) – Silicone mat – Personal card for use with the Mastercycler 384 – Oil (25 ml) – Bag with 4 spacers

3.2 Setup

The temperature sensor is commissioned for use in the temperature validation system as described in the operating manual of the temperature validation system (Section 6).

3.3 Accuracy of the measuring device and the measurements

The speciﬁcations from the operating manual of the temperature validation system apply.

Product information

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