Eppendorf SOP User Manual

rating Procedure for Pipettes

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P

N)Operating Procedure for Pipettes

Eppendorf SOP

Standard Operating Procedure for Pipettes

Copyright

©

2013 Eppendorf AG, Hamburg. No part of this publication may be

reproduced without the prior permission of the copyright owner.

®

eppendorf
Eppendorf Multipette
Biomaster

, the Eppendorf logo, Eppendorf Reference®, Eppendorf Research®,

®

, Eppendorf Xplorer®, Varipette®, Varispenser®,

®

, Combitips® and PICASO® are registered trademarks of 

Eppendorf AG.

AESOP13640-08/052013

Table of contents

Eppendorf SOP

English (EN)

Table of contents

1 Test and pipette conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6

1.1 Balances . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6

1.1.1 Balance type . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6

1.1.2 Accuracy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6

1.1.3 Minimum requirements for balances . . . . . . . . . . . . . . . . . .6

1.2 Measuring station . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7

1.2.1 Evaporation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7

1.2.2 Measuring station . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7

1.2.3 Test room . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7

1.2.4 Temperature differences. . . . . . . . . . . . . . . . . . . . . . . . . . . .7

1.2.5 Test liquid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7

1.2.6 Operating Manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7

2 Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8

2.1 Scope of testing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8

2.1.1 Adjusting adjustable volume pipettes . . . . . . . . . . . . . . . . . . 8

2.1.2 Multi-channel pipettes . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8

2.1.3 Multipette . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8

2.1.4 Bottle-top dispenser and Top Buret . . . . . . . . . . . . . . . . . . .9

2.2 Measurement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

2.2.1 Work method. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

2.2.2 Removing the test volume from the reservoir. . . . . . . . . . . . 9

2.2.3 Dispensing the test volume into the weighing vessel . . . . . 10

3

3 Evaluation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .11

3.1 Calculating the systematic error . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

3.2 Calculating the random error . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .11

4 Sterilization and cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .12

4.1 Sterilization . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

4.1.1 Reference and Biomaster . . . . . . . . . . . . . . . . . . . . . . . . . .12

4.1.2 Research plus and Reference 2. . . . . . . . . . . . . . . . . . . . . . 12

4.1.3 Research and Research pro . . . . . . . . . . . . . . . . . . . . . . . .13

4.1.4 Top Buret . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .13

4.1.5 Varipette and Multipette . . . . . . . . . . . . . . . . . . . . . . . . . . .13

4.1.6 Varispenser . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .13

4.1.7 Xplorer and Xplorer plus . . . . . . . . . . . . . . . . . . . . . . . . . .14

4.2 Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

Table of contents

4

Eppendorf SOP
English (EN)

5 Leakage check . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

5.1 Pipette leakage check . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .15

6 Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .15

6.1 Potential error causes and corrections . . . . . . . . . . . . . . . . . . . . . . . 15

7 Adjustment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .17

7.1 General information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .17

7.2 Adjusting adjustable-volume pipettes. . . . . . . . . . . . . . . . . . . . . . . .17

7.2.1 Procedure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .18

7.2.2 Biomaster . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

7.2.3 Reference variable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .19

7.2.4 Reference 2 adjustable-volume. . . . . . . . . . . . . . . . . . . . . .20

7.2.5 Research variable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .24

7.2.6 Research plusadjustable volume - adjustment to

environmental parameters. . . . . . . . . . .. . . . . . . . . . .. . . . . . 25

7.2.7 Research plus adjustable volume - change to the factory

adjustment.. . . . . . . . .. . . . .. . . . . . . . . . .. . . . . . . . . . . . . . . .26

7.2.8 Research Multi-channel . . . . . . . . . . . . . . . . . . . . . . . . . . .31

7.2.9 Research plus Multi-channel . . . . . . . . . . . . . . . . . . . . . . .31

7.2.10 Varispenser plus . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .32

7.2.11 Xplorer and Xplorer plus . . . . . . . . . . . . . . . . . . . . . . . . . .32

7.3 Adjusting fixed-volume pipettes. . . . . . . . . . . . . . . . . . . . . . . . . . . .33

7.3.1 Reference fixed . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .33

7.3.2 Reference2 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .34

7.3.3 Research fixed. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34

7.3.4 Research plus fixed. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .35

7.4 Physical influences of liquids . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .35

8 Factor Z for distilled water . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .36

8.1 Factor Z overview table. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .36

Table of contents

Eppendorf SOP

English (EN)

9 Technical specifications. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37

9.1 Fixed-volume pipettes. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .37

9.1.1 Reference fixed . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .37

9.1.2 Reference 2 fixed . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .38

9.1.3 Research fixed. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39

9.1.4 Research plus fixed. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .40

9.2 Adjusting adjustable volume pipettes. . . . . . . . . . . . . . . . . . . . . . . .41

9.2.1 Reference variable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41

9.2.2 Reference 2 adjustable-volume. . . . . . . . . . . . . . . . . . . . . .42

9.2.3 Research adjustable-volume . . . . . . . . . . . . . . . . . . . . . . . .44

9.2.4 Research pro . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .45

9.2.5 Research plus adjustable-volume . . . . . . . . . . . . . . . . . . . .46

9.2.6 Biomaster . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47

9.2.7 Varipette . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47

9.2.8 Xplorer and Xplorer plus . . . . . . . . . . . . . . . . . . . . . . . . . .48

9.3 Multi-channel pipettes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49

9.3.1 Research . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49

9.3.2 Research pro . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .50

9.3.3 Research plus . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .51

9.3.4 Reference2 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .52

9.3.5 Xplorer and Xplorer plus . . . . . . . . . . . . . . . . . . . . . . . . . .53

9.4 Multipette . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54

9.4.1 MultipettePlus . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54

9.4.2 MultipetteM4 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55

9.4.3 Multipette stream / Multipette Xstream . . . . . . . . . . . . . . .56

9.5 Varispenser / Top Buret . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57

9.5.1 Varispenser and Varispenser plus. . . . . . . . . . . . . . . . . . . .57

9.5.2 Top Buret . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .57

9.6 Error limits in accordance with EN ISO 8655. . . . . . . . . . . . . . . . . . 57

9.6.1 Air-cushion pipettes, fixed and adjustable volume. . . . . . .58

9.6.2 Positive displacement pipettes (Biomaster) . . . . . . . . . . . .58

9.6.3 Dispenser (Multipette) . . . . . . . . . . . . . . . . . . . . . . . . . . . .59

9.6.4 Single-stroke dispenser (Varispenser) . . . . . . . . . . . . . . . .60

9.6.5 Piston stroke burets . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .60

5

Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .61

Test and pipette conditions

6

Eppendorf SOP
English (EN)

1 Test and pipette conditions

These test and pipetting conditions are valid for all Eppendorf pipettes
and dispensers.
Follow EN ISO 8655 "Volume-measuring equipment with pistons".

For the calibration or adjustment of pipettes, the scales and measuring station
should fulfill the following requirements:

1.1 Balances

1.1.1 Balance type

Use semi-microbalances and microbalances to calibrate pipettes.

Some companies offer balances that are specially designed to meet the
requirements of pipette calibration, e.g. Sartorius and Mettler.

1.1.2 Accuracy

When selecting the balances, ensure that they are suitable for the accuracy of
the pipette. This helps to ensure that deviations within a measuring series are
recorded exactly for the assessment of the systematic and random measurement
deviations in accordance with EN ISO 8655.

For a pipette volume of less than or equal to 10 µl, balances with a 6-digit
display must be used. With larger volumes, balances with a 5-digit display are
sufficient.

1.1.3 Minimum requirements for balances

Selected volume* of the pipette to be
tested

1 µL to 10 µL 0.001
>10 µL to 100 µL 0.01
>100 µL to 1 000 µL 0.1
>1 mL to 10 mL 0.1
>10 mL to 200 mL 1

* For practical reasons, the nominal volume can be used for the selection of the
balances.

Display resolution (mg)

Test and pipette conditions

Eppendorf SOP

English (EN)

1.2 Measuring station

1.2.1 Evaporation

Take evaporation protection into account while carrying out the measurement.

With volumes < 50 µl in particular, errors caused by the evaporation of the test
liquid must be taken into consideration.

This can be ensured by using a liquid trap or other equipment to prevent
evaporation.

1.2.2 Measuring station

For electronic measurement data processing, we recommend calibration
software and accessories for your balance (PICASO, order no. 3113 004.001).

(see also EN ISO 8655, Part 6)

1.2.3 Test room

The tests should be carried out in a draft-free room under constant climatic
conditions.

The test room should have a constant temperature between 15 °C and 30 °C and
a constant relative humidity above 50 %.

7

1.2.4 Temperature differences

Before the test, the device to be tested and test liquid must have stood in the test
room for a sufficient amount of time, at least 2 hours, in order to reach
equilibrium with the storage conditions.

Direct sunlight and other influences which could affect the temperature should
be avoided at all costs.

1.2.5 Test liquid

Distilled or deionized water of "Quality 3 in accordance with ISO 3696",
degassed or at equilibrium with air. The water must be at room temperature.

1.2.6 Operating Manual

Observe the operating instructions for your pipette.

Calibration

8

Eppendorf SOP
English (EN)

2 Calibration

2.1 Scope of testing

Note concerning the nominal volume:

The nominal volume of an adjustable-volume pipette is the largest volume to be
set by the user and specified by the manufacturer.

When using the Combitip with the Multipette, the nominal volume is the largest
possible dispensing volume of the Combitip. This means that for the Multipette
plus and Multipette M4, the nominal volume is 1/5 of the filling volume of the
Combitip advanced. For the Multipette stream / Xstream, the nominal volume is
the filling volume of the Combitip advanced.

2.1.1 Adjusting adjustable volume pipettes

For variable pipettes, check 3 different volumes with 10 measured values each:

• the nominal volume,

• approx. 50 % of the nominal volume,

• 10 % of the nominal volume.

2.1.2 Multi-channel pipettes

For Multi-channel pipettes check each channel separately with 3 different
volumes, each with 10 measured values:

• the nominal volume,

• approx. 50 % of the nominal volume,

• 10 % of the nominal volume.

2.1.3 Multipette

With the

• Multipette,

• Multipetteplus,

• Multipette stream,

• MultipetteXstream

• MultipetteM4

check the nominal volume with 10 measured values with the Eppendorf
Combitip used.

Calibration

Eppendorf SOP

English (EN)

2.1.4 Bottle-top dispenser and Top Buret

For bottle-top dispensers and the Top Buret, check the nominal volume with 10
measured values.

2.2 Measurement

2.2.1 Work method

1. Place the selected pipette tip on the tip cone of the pipette or on the
corresponding Combitip in the Multipette.

2. Implement the following settings:

• Adjustable-volume piston-stroke pipettes: the smallest volume to be tested

• Multipettes: the nominal volume

• Bottle-top dispenser and Top Buret: the nominal volume

3. For all Multipettes with fully drawn up Combitip, always discard the first
dispensing step.

4. Fill test liquid up to a height of min. 3 mm in the weighing vessel.

5. For piston-stroke pipettes fill the pipette tip 5x with test liquid and empty it
(pre-wet), in order to create a moisture balance in the dead air volume.

6. Replace the single-use tip.

7. Pre-wet the tip 1x.

9

2.2.2 Removing the test volume from the reservoir

1. Hold the pipette vertically.

2. Dip the pipette tip into the test liquid by a few millimeters.

3. Draw in the volume to be tested slowly and evenly. The waiting time of 1 to
3 seconds, for Research 1 to 10 mL 5 seconds must be observed.
(the waiting time corresponds to the size of the tip, see operating
instructions.)

4. Pull the pipette tip slowly out of the liquid, wiping it on the vessel wall.

Calibration

10

Eppendorf SOP
English (EN)

2.2.3 Dispensing the test volume into the weighing vessel

1. Rest the filled tip up against the wall of the weighing vessel at an angle.

2. Dispense the test liquid slowly until the first stop (measuring stroke).

3. Press the control button to the second stop (blow-out) and dispense the
remaining liquid in the tip (does not apply for dispensers and burets).

4. Hold down the control button and pull the tip up the vessel wall.

5. Let the control button slide back into position.

6. Determine the weight.

7. Complete all measurements of a measuring series as described and calculate
the systematic and random error (see p. 11).

8. In case of adjustable-volume pipettes, determine the measurement with the
nominal volume, 50 % and 10 % of the nominal volume. Always begin the
test with 10 % of the nominal volume.

Evaluation

Eppendorf SOP

English (EN)

3 Evaluation

3.1 Calculating the systematic error

Mean value of the dispensed volume:

n = Number of measured values

To convert the measured values into volume values, use correction factor Z for
the dependency of the test liquid on temperature and air pressure for each
individual value (see Factor Z for distilled water on p. 36).

11

Systematic error e

Systematic error e

in micro liter:

s

in percent:

s

3.2 Calculating the random error

Random error as repeat standard s:

Random error as coefficient of variation CV:

The specifications of the tested pipette can be found in the relevant operating
instructions or technical specifications (see Technical specifications on p. 37).

Sterilization and cleaning

12

Eppendorf SOP
English (EN)

4 Sterilization and cleaning

Further notes on cleaning and sterilization can found in the relevant
operating manual.

4.1 Sterilization

Parameter for autoclaving

• 121 °C

• 20 minutes

• 1 bar overpressure

4.1.1 Reference and Biomaster

The Reference pipettes and the Biomaster are fully autoclavable.

1. Prior to autoclaving, unscrew the upper and lower part of the pipette apart by
approx. one turn to enable the vapor to enter more easily.

2. After autoclaving, allow the pipette to cool down to room temperature and to
dry completely and then screw together.

4.1.2 Research plus and Reference 2

The Research plus pipettes and Reference 2 pipettes are fully autoclavable.

1. You can put the Research plus and Reference 2 into the autoclave as a
complete unit or with the lower part removed. Do not disassemble the lower
part.

2. For 2.5 mL, 5 mL and 10 mL pipettes: remove the old protection filter. Add a
new protection filter and install it after autoclaving. Only autoclave the
protection filter once.
After autoclaving:

3. Cool the pipette down to room temperature and leave to dry.

4. For 2.5 mL, 5 mL / 10 mL pipettes: the protection filter swells during
autoclaving. Slightly squeeze the protection filter when installing it into the
cone tip.

Sterilization and cleaning

Eppendorf SOP

English (EN)

4.1.3 Research and Research pro

With the Research and Research pro pipettes, the lower section is autoclavable.

With single-channel models prior to autoclaving

1. Pull off the ejector sleeve with depressed ejector.

2. Unscrew the lower part of the pipette.

With the Multi-channel versions, you can autoclave the entire lower section.

After autoclaving, assemble the parts only after room temperature has been
reached and the parts have been dried.

4.1.4 Top Buret

The Top Buret can not be autoclaved.

4.1.5 Varipette and Multipette

The

• Varipette,

• Multipette,

• Multipetteplus,

• Multipette stream

• MultipetteXstream

• MultipetteM4

13

are not autoclavable.

4.1.6 Varispenser

The Varispenser and Varispenser plus are only autoclavable when fully
assembled.

With theVarispenser plus

1. Set the discharge valve toggle to the dispensing position (–>).

2. Release the volume setting knob, move to the middle position and leave
released.

3. Place the dispenser on a cloth and autoclave, avoiding contact with hot
surfaces.

4. Only use the dispenser again when it has cooled down to room temperature.

Sterilization and cleaning

14

Eppendorf SOP
English (EN)

4.1.7 Xplorer and Xplorer plus

The lower part of theXplorer and Xplorer plus pipettes can be autoclaved.

1. Pull off the ejector sleeve with depressed ejector.

2. On the lower part of the pipette, push the ring labeled PUSH UP TO
RELEASE upward until the lower part is released.

After autoclaving, assemble the parts only after room temperature has been
reached and the parts have been dried.

4.2 Cleaning

All areas of the underside of the pipette can be cleaned in a soap
solution or isopropanol 60 % provided that no differing instructions are
given in the operating manual.

1. Clean the parts in soap solution or isopropanol.

2. Rinse the parts in distilled water.

3. Allow the parts to dry completely and then assemble.

4. Lightly lubricate the piston of the pipette (Eppendorf special grease

Leakage check

Eppendorf SOP

English (EN)

5 Leakage check

5.1 Pipette leakage check

In order to carry out a leakage check on a pipette, follow the procedure outlined
below:

1. For adjustable-volume pipettes: set the nominal volume.

2. For volumes <20 µl pre-wet the tip several times.

3. Hold the pipette vertically with a full tip for approx. 30 sec. Do not touch the
pipette tip.

4. Observe the meniscus of the liquid on the tip opening. If there is a leak in the
pipette, a drip will form on the tip opening.

6 Troubleshooting

6.1 Potential error causes and corrections

Observe the error descriptions in the operating instructions.

Error Cause Elimination

Droplets on the
inner wall of the
pipette tip.

Pipette is dripping,
pipetted volume is
incorrect.

Uneven wetting of the plastic
wall.

• Tip loose.

• Incorrect pipette tip.

Pipette is leaking because:

•The piston is
contaminated.

• The piston is damaged.

• The seal is damaged.

 Attach a new pipette tip.

 Press the tip on firmly.
 Use an original Eppendorf

tip.

 Clean the piston and

lubricate slightly.

 Replace the piston and

piston sealing and
lubricate slightly.

 Replace the seal.

15

Troubleshooting

16

Eppendorf SOP
English (EN)

Error Cause Elimination

Liquid cannot be
aspirated correctly
or dripping occurs.

• Leakage in the Combitip
advanced.

• Combitip advanced has
heated up.

 Replace the Combitip

advanced with a new
Combitip advanced.

 Ensure that the

temperature is uniform as
the liquid expands when
heated.

The control button
is jammed, runs
jerky.

•The piston is
contaminated.

• The seal is contaminated.

 Clean the piston and

lubricate slightly.

 Disassemble the pipette,

clean all seals, replace if
necessary.

• The piston is damaged.

 Replace the piston and

piston seal and lubricate
slightly.

• Penetration of solvent
vapors

 Unscrew the lower part of

the pipette and aerate the
lower part.

 Clean the piston and

lubricate slightly.

Adjustment

Eppendorf SOP

English (EN)

7Adjustment

7.1 General information

Prior to delivery, all pipettes are adjusted with distilled or de-ionized, degassed
water under the conditions described in Chapters 1 to 3 and according to ISO
3696 (see Calibration on p. 8).

If you have doubts regarding the accuracy of the volume entered, please check
the following points first:

• Is there a leak in the device (see Leakage check on p. 15)?
Exception: Biomaster

• Does the temperature of the pipetted liquid correspond to:
– the temperature of the device?
– the ambient air temperature?

• Is the set volume correct?

• Has the liquid density and air pressure been taken into consideration?

• Does the density of the pipetted liquid deviate from the double-distilled,
degassed water?

• Was the work completed correctly, as described in the "Calibration" chapter
(see p. 8) and "Evaluation" chapter (see p. 11)?

• Were original Eppendorf pipette tips used?

Volume errors can also occur when pipetting liquids with high vapor pressure,
where the density or viscosity deviates considerably from the values of water.

Adjustments should only be carried out on the device after these conditions have
been taken into consideration.

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7.2 Adjusting adjustable-volume pipettes

Observe the operating instructions for your pipette. There you will find
detailed descriptions on adjustment.

The adjustment of the following adjustable-volume pipettes is a zero point offset:

• Research

• Research plus

• Reference

•Biomaster

• Reference2

Adjustment

18

Eppendorf SOP
English (EN)

7.2.1 Procedure

Requirements

Device, original Eppendorf tip, test liquid and ambient air must have the same
temperature (15 – 30°C) at a constant temperature of ±0.5°C during the test
(according to EN ISO 8655, Part 6).

If your Research, Research plus or Reference has an adjustment, remove
it before carrying out the adjustment.
After adjusting, lock the adjustment opening with a new adjustment
seal.
Adjustment seals can be reordered (see operating manual).

1. Set the pipette to the smallest volume to be tested.

2. Use a matching original Eppendorf tip for single-channel pipettes. For
Multi-channel pipettes, use any channel.

3. Pipette the set volume 10 times.

4. Conduct a weighing after every volume dispensing.

5. Calculate the mean value after 10 measurements (see Evaluation on p. 11).
The calculated mean value of these weighings (observe conversion factor Z =
mg to µL) yields the actual volume.

6. For adjusting, introduce the suitable tool into the opening intended for this
purpose, or place it on the designated location, and adjust the actual volume
(see the following figures or operating manual).

7. Check the set volume by measuring again. If the target volume does not
match the result of the measurement, repeat steps 2 to 6.

8. After the adjustment, check the measured values for accuracy at 50% of the
nominal volume and check the nominal volume for accuracy.

7.2.2 Biomaster

1

0

2

0

Auxiliary equipment

• Supplied pipette wrench (order no. 4910 092.001)

1. Use the pipette wrench to adjust the
volume display of the pipette, with
the piston stroke unchanged, to the
actual volume value of the
measurement.

2. Pull off the pipette wrench.

3. Set the pipette to the target volume
in the usual manner.

4. Mark the adjustment on the pipette.

7.2.3 Reference variable

Adjustment

Eppendorf SOP

English (EN)

19

Auxiliary equipment

• Supplied pipette wrench (order no. 4910 092.001)

• Supplied red CAL seal

1. Insert side B of the pipette wrench
into the adjustment opening in the
lid.

0

6

2

6

2. Using the pipette wrench, set the
digital display of the pipette, with
unaltered piston stroke, to the actual
volume value of the measurement.

3. Pull off the pipette wrench.

4. Set the pipette to the target volume
in the usual manner.

5. After the adjustment has been
successfully completed, lock the
adjustment opening with a red CAL
seal.

Adjustment

Reference 2

ADJ

Reference 2

Reference 2

20

Eppendorf SOP
English (EN)

7.2.4 Reference 2 adjustable-volume

7.2.4.1 User adjustment

Changing the adjustment will change the volume by a specific value. Strictly
speaking, the change only applies to the testing volume.

Example:

You readjust a 10 – 100 µL pipette, with a volume setting of 100 µL, by 1 µL
(1 µL  1%). If the volume setting is 10 µL, the pipette is also adjusted by 1 µL
( 10%).

Auxiliary aids from the delivery package

•Pin

• Red plastic adjustment seal (ADJ)

1. Insert the pin in the middle of the gray adjustment seal (ADJ).

2. Remove the adjustment seal.

3. Insert the adjustment tool.

4. Turn the adjustment tool until the adjustment display shows the desired
value.

5. Read the set value distortion-free using the aligning aid in the viewing
window.

6. Carry out weighings to check the accuracy and precision.

1000 µL

ADJ

+-

11