Instructions for use Eppendorf Serological Pipetsseeon p.Fig.Tab.p.
10 mL in 1/10 TD 20° C
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10 mL in 1/10 TD 20° C
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English (EN)
Instructions for use
Eppendorf Serological Pipets
English (EN)
1 Operating instructions
1.1 Using this manual
Before using the consumables for the first time, read these instructions for use and the operating manual of
the device that you use the consumables with.
2 Product description
2.1 Main illustration
1Outlet opening
2Graduation
3Zero point
4 Negative graduation
5Nominal volume
7Pipette type
8 Test temperature
9 Color code
10 Filter
11 Aspiration area
6 Increment
2.2 Overview of Eppendorf Serological Pipets with color codes
Nominal volume 1 mL 2 mL 5 mL 10 mL 25 mL 50 mL
Color code
Accuracy [± mL] 0.02 0.04 0.1 0.2 0.5 1
Graduation [mL] 0.01 0.02 0.1 0.1 0.2 0.5
Negative graduation [mL] -0.2 -0.8 -3 -3 -9 -6
Length [mm] 277 277 338 338 343 343
Diameter at the aspiration area [mm]
4.6 6.4 8.2
Max. diameter [mm] 9.9 14.6 18.6
7.8 8.3 8.3
Instructions for use
Eppendorf Serological Pipets
2
English (EN)
2.3 Features
Eppendorf Serological Pipets are single-use items for aspiring and dispensing liquids using manual and
electronic pipette controllers (e.g., Eppendorf Easypet 3 and Eppendorf Pipet Helper). The pipettes are
calibrated to blow-out. The various pipette sizes are marked with a color code.
2.4 Materials
NOTICE! Aggressive substances may damage the Eppendorf Serological Pipets and
accessories.
Check the resistance to chemicals before using organic solvents or aggressive chemicals.
Material
Pipette Polystyrene (PS)
Filter Polyethylene (PE)/polyester (PES)
2.5 Resistance to chemicals
■■■ Resistant
The chemical can be used.
■■ Limited resistance and/or suitable for limited use
The chemical can be used for a limited period of time. Dispense
immediately after liquid aspiration. Extended contact may damage
the Eppendorf Serological Pipets.
■ Unstable
Material and prinitng are unstable. The chemical cannot be used.
Solution Concentration in % Resistance
Ammonia solution 25 ■■
Acetic acid 12 ■
Caustic soda 20 ■
Nitric acid 65 ■
Sulfuric acid 16 ■
Acetone ■
Acetonitrile ■
Chloroform ■
Dimethyl sulfoxide 100 ■■■
Ethanol 70 ■■
Isoamyl alcohol ■■
Isopropanol 70 ■■
Instructions for use
Eppendorf Serological Pipets
English (EN)
Solution Concentration in % Resistance
Methanol ■
Formaldehyde 37 ■■
Glutaraldehyde ■■■
Glycerol 99.6 ■■■
Triton X-100 1 ■■■
DMEM ■■■
Fetal bovine serum ■■■
Water ■■■
3Safety
3.1 Intended use
In combination with pipette controllers Eppendorf Serological Pipets are intended for dispensing liquids
within a volume range of 0.1 mL to 50 mL.
3
Eppendorf Serological Pipets can be used for training, routine and research laboratories in the areas of life
sciences, industry or chemistry. This product is intended to be used for research purposes only. Eppendorf
does not provide a warranty for other applications. Not suitable for use in diagnostic or therapeutic
applications. Eppendorf Serological Pipets may only be used by skilled personnel who have been trained in
the areas mentioned above.
The user must carefully read the instructions for use and the operating manual of the pipette controller
used and become familiar with how the device works.
Instructions for use
Eppendorf Serological Pipets
4
English (EN)
3.2 Warnings for intended use
WARNING! Damage to health from toxic, radioactive or aggressive chemicals as well as
infectious liquids and pathogenic germs.
Observe the national regulations for handling these substances, the biological security
level of your laboratory, the material safety data sheets and the manufacturer's application
notes.
Wear personal protective equipment.
For full instructions regarding the handling of germs or biological material of risk group II
or higher, please refer to the "Laboratory Biosafety Manual" (Source: World Health
Organization, current edition of the Laboratory Biosafety Manual).
Eppendorf Serological Pipets are not autoclavable.
4 Operation
To ensure maximum protection against contamination use Eppendorf Serological Pipets
immediately after opening the packing.
1. Open the packing at the location indicated.
2. Insert the pipette carefully into the corresponding opening of the pipette controller until the pipette
seals airtight.
3. Immerse the pipette into the liquid.
4. Fill the pipette up to above the desired dispensing volume.
5. Set the lower meniscus to the desired dispensing volume.
6. Wipe the pipette on the inner wall of the source vessel and remove it.
7. Hold the pipette vertically and place it against the inner wall of the destination vessel.
8. Allow liquid to flow out.
9. Blow out residual volume.
• Observe the lower meniscus while dispensing.
Instructions for use
Eppendorf Serological Pipets
English (EN)
5 Technical data
5.1 Ambient conditions
Air temperature Relative humidity Atmospheric pressure
5 °C – 40 °C 10 % – 95 % 70 kPa – 106 kPa
5.2 Transport
Air temperature Relative humidity Atmospheric
pressure
General transport -25 °C – 60 °C 10 % – 95 % 30 kPa – 106 kPa
Air freight -40 °C – 45 °C 10 % – 95 % 30 kPa – 106 kPa
5.3 Storage
Air temperature Relative humidity Atmospheric
pressure
In transport packaging -25 °C – 55 °C 10 % – 95 % 70 kPa – 106 kPa
Without transport packaging -5 °C– 45 °C 10 % – 95 % 70 kPa – 106 kPa
5
6 Purity grades and certificates
6.1 Purity
Eppendorf Serological Pipets are sterile, free of pyrogens, RNase, DNase, DNA, and not cytotoxic.
You can find the date of expiry on the label of the folding box and on the blister packing.
6.2 Certificates
You can download various certificates for Eppendorf consumables from our webpage www.eppendorf.com/
consumables.
• Lot-specific certificates:
For Eppendorf Consumables lot-specific certificates are available. You can find the lot number on the
label of the folding box and on the blister packing of the single-use item.
• General certificates of conformity
• ISO Certificates
Instructions for use
Eppendorf Serological Pipets
English (EN)
7 Ordering information
7.1 Serological pipettes
Order no.
(International)
Order no. (North
America)
Description
Eppendorf Serological Pipet, 1 mL
0030 127.692 0030127692 4 boxes of 200 (800 pieces)
Eppendorf Serological Pipet, 2 mL
0030 127.706 0030127706 4 boxes of 150 (600 pieces)
Eppendorf Serological Pipet, 5 mL
0030 127.714 0030127714 4 boxes of 100 (400 pieces)
Eppendorf Serological Pipet, 10 mL
0030 127.722 0030127722 4 boxes of 100 (400 pieces)
Eppendorf Serological Pipet, 25 mL
0030 127.730 0030127730 4 boxes of 50 (200 pieces)
Eppendorf Serological Pipet, 50 mL
0030 127.749 0030127749 4 boxes of 40 (160 pieces)
7.2 Pipette controllers
Order no.
(International)
4430 000.018 – Easypet 3
– 4430000026 Easypet 3
4423 000.010 4423000010 Eppendorf Pipet Helper
Order no. (North
America)
Description
Incl. power supply, wall mount, depositing stand, 2 membrane
filters (unsterile) 0.45 μm
Incl. power supply, wall mount, 2 membrane filters (unsterile)
0.45 μm
Your local distributor: www.eppendorf.com/contact
Eppendorf AG · 22331 Hamburg · Germany
eppendorf@eppendorf.com · www.eppendorf.com
and the Eppendorf logo are registered trademarks of Eppendorf AG, Hamburg, Germany.
®
Eppendorf
0012 585.950-01/092014
©2014 ·
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