6320 071.168-01/0611
Self Test Function
for Mastercycler® pro and
Mastercycler® nexus
Instructions for use
Copyright
©
2011 Eppendorf AG, Hamburg. No part of this publication may be reproduced without
the prior permission of the copyright owner.
Trademarks
eppendorf, Mastercycler and vapoprotect are registered trademarks of Eppendorf AG, Hamburg,
Germany.
Registered trademarks are not marked in all cases with ™ or
®
in this manual.
6320 071.168-01/062011
Self Test Function for Mastercycler® pro and Mastercycler® nexus
Instructions for use — Self Test Function for Mastercycler® pro and Mastercycler® nexusseeon p.Fig.Tab.p.
Instructions for use
Table of contents
1 Table of contents
1 Operating instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
1.1 Using this manual. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
2 Product description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
2.1 Delivery package . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
2.2 Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
3 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
3.1 Activating the self-test function. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
3.2 Performing the self-test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
3.2.1 Creating a certificate. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
3.3 Displaying, printing or exporting a history . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
3.3.1 Printing a history . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
3.3.2 Exporting a history as a file. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
4 Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
5 Transport, storage and disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
5.1 Disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
6 Ordering Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
3
Self Test Function for Mastercycler® pro and Mastercycler® nexus
1 Operating instructions
1 Operating instructions
1.1 Using this manual
These instructions for use for the self-test function of the Mastercycler pro and Mastercycler
nexus is a supplement to the Thermocycler operating instructions and does not replace the
Thermocycler instructions.
Therefore, the Thermocycler operating instructions must be read before using the self-test
function for the first time. The current version of the instructions is available on the internet at
www.eppendorf.com
2 Product description
2 Product description
2.1 Delivery package
Quantity Order No.
1 6320 071.001 950030040
1
(International)
6320 071.168-
Order No.
(North America)
Description
Selftest USB Key
For Mastercycler pro and Mastercycler nexus
Mastercycler pro Self Test Function Instructions for Use
2.2 Features
The self-test function can be used with the following devices:
• Mastercycler pro family of devices and the 6320 control panel (with USB ports)
• Mastercycler nexus family of devices
Eliminating the need for an external measuring system, the self-test function is used to quickly
check of the following thermoblock characteristics:
• Heating and cooling rates
• Correct function of the temperature control circuit
• Temperature homogeneity within each of the three block zones (left, center and right zone)
• Temperature homogeneity across the entire block
A certificate can be output as a PDF after the self-test has been completed successfully.
The self-test USB key also activates the self-test function for all connected Mastercyclers.
3Operation
3 Operation
3.1 Activating the self-test function
Requirement
• A user is logged on (if the PIN function is activated).
• The navigation tree is displayed.
Connect the self-test USB key to an open USB port on the Thermocycler.
The message Please wait appears. The self-test USB key is detected after a few seconds.
The self-test function is now unlocked.
4
Self Test Function for Mastercycler® pro and Mastercycler® nexus
The status of the self-test function can be identified using the symbol in the navigation tree below
the cycler node.
• Self-test function activated
• Self-test function locked
The self-test function remains active until the self-test USB key is removed.
3.2 Performing the self-test
CAUTION! Risk of burns from hot thermoblock and heated lid.
The thermoblock and the heated lid very quickly reach temperatures above 95 °C.
CAUTION!
Keep heated lid closed until the temperature of the thermoblock is below 30 °C.
Requirement
• A user is logged on (if the PIN function is activated).
• The navigation tree is displayed.
• The self-test function is unlocked (see p. 4).
1. Mount the thermoblock with an empty PCR plate and close the heated lid.
2. Open the cycler node.
3. Mark the Self-test node.
4. Click the Start softkey.
5. Click the Start softkey again.
The self-test runs automatically. Information on the currently running test is displayed.
5
Self Test Function for Mastercycler® pro and Mastercycler® nexus
Block zone The left, center and right block zones correspond to the
Heating/cooling rates Temperature control velocity of the block during heating or
Temperature control Accuracy of thermoregulation.
Temperature homogeneity Temperature homogeneity within each individual control circuit.
Overall temp. homogeneity Temperature homogeneity across the entire block.
At block temperatures of 4 °C, 35 °C, 55 °C, 75 °C and 95 °C, the temperature
homogeneity and thermoregulation accuracy are determined after 30 s of holding time.
After 5 to 10 minutes the test result is displayed.
temperature control loop of the block.
cooling.
• Passed tests are labeled PASS and highlighted in green.
• Failed tests are labeled FAIL and highlighted in red.
The self-test is passed if all subtests have been passed.
Failure of a test indicates an alteration, maladjustment or malfunctioning of the block. In
Hint!
this case, please contact Eppendorf Service.
Use the Eppendorf Temperature Verification System to verifiy and adjust the
Hint!
temperature control of the block (see on p. 10).
6
Self Test Function for Mastercycler® pro and Mastercycler® nexus
3.2.1 Creating a certificate
After the self-test has been passed, a certificate can be exported to an external storage medium
as a PDF.
Requirement
The self-test has passed all subtests and the test result is displayed.
1. Connect a storage medium.
2. Click the Certificate softkey.
The certificate is copied onto the storage medium as a PDF file. The path of the copied files
is displayed after the procedure has been completed.
You can display the certificate on a PC and print it using Adobe
viewer.
The certificate contains fields to enter a validity date and written signature.
Abb. 1:Example of a self- test certificate fo r a Mastercycler pro.
®
Reader® or a different PDF
Fig. 1: Example of a self-test certificate for a Mastercycler pro.
3.3 Displaying, printing or exporting a history
Use of the self-test function and the results are recorded in a log file. To display, print or export
the log file to a storage medium, proceed as follows:
7
Self Test Function for Mastercycler® pro and Mastercycler® nexus
Requirement
• A user is logged on (if the PIN function is activated).
• The navigation tree is displayed.
1. Open the cycler node and mark Self-test.
2. Click the History softkey.
The self-test history is displayed.
3.3.1 Printing a history
Requirement
A supported printer is connected (see Cycler operating instructions).
Click the Print softkey.
3.3.2 Exporting a history as a file
1. Connect an external storage medium.
2. Click the Export softkey.
3. Select the PDF or TXT file format and confirm by clicking Ok.
The file is copied to the storage medium. The path of the copied files is displayed after the
procedure has been completed.
4 Troubleshooting
4 Troubleshooting
Symptom/
message
Self-test USB key is
not detected.
8
Cause Remedy
The self-test USB key was
connected to a non-supported USB
hub.
The control panel USB connections
are not active because the control
panel was connected to a
Mastercycler ep (the symbol is
displayed).
Connect the self-test USB key
immediately.
Connect the control panel to a
Mastercycler pro immediately (see
Mastercycler pro operating
instructions).
Self Test Function for Mastercycler® pro and Mastercycler® nexus
Symptom/
message
The self-test node
is not displayed.
Cause Remedy
The USB connections were
switched off due to a damaged or
incompatible USB device (the
symbol is displayed).
No user is logged on.
A Mastercycler ep series device is
selected.
Disconnect all USB devices and
restart the Cycler.
Log on as user or administrator.
Select a Mastercycler pro node.
5 Transport, storage and disposal
5 Transport, storage and disposal
5.1 Disposal
In case the product is to be disposed of, the relevant legal regulations are to be observed.
Information on the disposal of electrical and electronic devices in the European
Community:
Within the European Community, the disposal of electrical devices is regulated by national
regulations based on EU Directive 2002/96/EC pertaining to waste electrical and electronic
equipment (WEEE).
According to these regulations, any devices supplied after August 13, 2005, in the
business-to-business sphere, to which this product is assigned, may no longer be disposed of in
municipal or domestic waste. To document this, they have been marked with the following
identification:
Because disposal regulations may differ from one country to another within the EU, please
contact your supplier if necessary.
In Germany, this is mandatory from March 23, 2006. From this date, the manufacturer has to offer
a suitable method of return for all devices supplied after August 13, 2005. For all devices
supplied before August 13, 2005, the last user is responsible for the correct disposal.
9
Self Test Function for Mastercycler® pro and Mastercycler® nexus
6 Ordering Information
6 Ordering Information
Order No.
(International)
6320 071.001 950030040
6321 900.150 - Operating Manual Mastercycler pro
6331 900.069 6331900069 Operating Manual Mastercycler nexus
6320 071.168 - Mastercycler pro Self Test Function - Instructions for Use
0055 000.298 950008059 Temperature Verification System
5331 233.007 on request Sensor for 384 well block
Order No.
(North America)
Description
Selftest USB Key
For Mastercycler pro and Mastercycler nexus
10
Eppendorf offices
Your local distributor: www.eppendor f.com/worldwide
Eppendorf AG · 22331 Hamburg · Germany · Tel: +49 40 53801-0 · Fax: +49 40 538 01-556 · E-mail: eppendorf@eppendorf.com
Eppendorf North America, Inc. · 102 Motor Parkway · Hauppauge, N.Y. 11788-5178 · USA
Tel: +1 516 334 7500 · Toll free phone: +1 800-645-3050 · Fax: +1 516 334 7506 · E-mail: info@eppendorf.com
Application Support Europe: Tel: +49 1803 666 789 (Preis je nach Tarif im Ausland; 9 ct/min aus dem dt. Festnetz; Mobilfunkhöchstpreis 42 ct/min)
support@eppendorf.com
North America: Tel: +1 800 645 3050 · E-mail: techserv@eppendorf.com
Asia Pacific: Tel: +60 3 8023 6869 · E-mail: support_asiapacific@eppendorf.com
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