Eppendorf 5430 R User Manual

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Centrifuge 5430/5430 R

Operating manual

©

Copyright

2013 Eppendorf AG, Hamburg. No part of this publication may be reproduced without the prior

permission of the copyright owner.

Trademarks

Eppendorf

®

, the Eppendorf logo. CombiSlide®, and QuickLock® are registered trademarks of

Eppendorf AG, Hamburg, Germany.

Centriplus

Microtainer

Trademarks are not marked in all cases with ™ or

®

is a registered trademark of Millipore Corporation, Billerica, USA.

®

is a registered trademark of Becton Dickinson, Franklin Lakes, NJ, USA.

®

in this manual.

Operating manual. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3

Declarations and Certificates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69

Menu structure of Centrifuge 5430/5430 R . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85

Rotor F-35-6-30: Adapters for round-bottom tubes and blood taking systems . . . . . . . . . . . . . . . . . . . . . . 86

5427 901.124-04/072013

Table of contents

Centrifuge 5430/5430 R

English (EN)

Operating manu alCentrifuge 5430/5430 Rseeon p.Fig.Tab.p.

English (EN)Operating manual

Table of contents

1 Operating instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

1.1 Using this manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

1.2 Danger symbols and danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

1.2.1 Danger symbols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

1.2.2 Danger levels. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

1.3 Symbols used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

1.4 Abbreviations used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

2 Product description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

2.1 Main illustration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

2.2 Delivery package. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

2.2.1 Centrifuge 5430. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

2.2.2 Centrifuge 5430 R . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

2.3 Features. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

2.4 Rotors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

2.4.1 rcf display and calculation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16

3

3 Safety. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18

3.1 Intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18

3.2 User profile . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18

3.3 Application limits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18

3.3.1 Declaration concerning the ATEX directive (94/9/EC) . . . . . . . . . . . . . . . . . . . . . . . . . 18

3.3.2 Maximum service life for accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18

3.4 Information on product liability . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

3.5 Warnings for intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

3.5.1 Personal injury or damage to the equipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

3.5.2 Incorrect handling of the centrifuge . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21

3.5.3 Incorrect handling of the rotors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

3.5.4 Extreme strain on the centrifuging tubes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23

3.5.5 Aerosol-tight centrifugation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24

3.6 Safety notes on the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24

4 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

4.1 Selecting the location . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

4.2 Preparing installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

4.3 Installing the instrument. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26

5 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28

5.1 Overview of operating controls . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28

5.2 Menu navigation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29

5.3 Configure centrifuge . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30

5.3.1 Set menu language . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30

5.3.2 Setting the date and time . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30

5.4 Preparing for centrifugation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32

5.4.1 Switching on the centrifuge . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32

5.4.2 Inserting the rotor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32

5.4.3 Automatic rotor detection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32

5.4.4 Manual rotor detection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32

5.4.5 Loading the rotor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

5.4.6 Closing the centrifuge lid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35

Table of contents

Centrifuge 5430/5430 R

4

English (EN)

5.5 Cooling (only 5430 R) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36

5.6 Centrifuging . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39

5.7 Standby mode . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41

5.8 User instructions on rotors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42

5.5.1 Temperature adjustment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36

5.5.2 Temperature display . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36

5.5.3 Temperature monitoring . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36

5.5.4 FastTemp. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36

5.5.5 FastTemp pro . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37

5.5.6 Continuous cooling . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38

5.6.1 Centrifugation with time setting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39

5.6.2 Centrifuging in continuous operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40

5.6.3 Short-spin centrifugation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40

5.6.4 Removing the rotor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41

5.8.1 Rotor F-35-6-30: rotor removal tool . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42

5.8.2 Rotor A-2-MTP . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43

5.8.3 Rotor FA-45-24-11-HS: using the special rotor key . . . . . . . . . . . . . . . . . . . . . . . . . . . 43

5.8.4 QuickLock . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44

6 Operating controls and function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45

6.1 Device menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45

6.2 Settings in the device menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47

6.2.1 Programs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47

6.2.2 Use program keys . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47

6.2.3 Other menu items . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48

6.2.4 Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49

7 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50

7.1 Prepare cleaning/disinfection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50

7.2 Cleaning/disinfection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50

7.2.1 Cleaning and disinfecting the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51

7.2.2 Cleaning and disinfecting the rotor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51

7.3 Additional service instructions for Centrifuge 5430 R . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52

7.4 Glass breakage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53

7.5 Fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53

7.6 Decontamination before shipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53

8 Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54

8.1 General errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54

8.2 Error messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55

8.3 Emergency release . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57

9 Transport, storage and disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58

9.1 Transport. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58

9.2 Storage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58

9.3 Disposal. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58

10 Technical data. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59

10.1 Power supply. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59

10.2 Ambient conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59

10.3 Weight/dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60

10.4 Application parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60

Table of contents

Centrifuge 5430/5430 R

English (EN)

11 Ordering Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62

11.1 Centrifuge 5430 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62

11.2 Centrifuge 5430 R . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62

11.3 Rotors, rotor lids and seals . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63

11.3.1 Rotors with QuickLock rotor lid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63

11.3.2 Rotors with rotor lid thread. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65

11.3.3 Rotors with rotor lid for attaching. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67

11.4 Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67

11.4.1 Adapter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67

11.4.2 Other accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68

11.4.3 Fuses for Centrifuge 5430. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68

5

Table of contents

Centrifuge 5430/5430 R

6

English (EN)

Operating instructions

Centrifuge 5430/5430 R

English (EN)

1 Operating instructions

1.1 Using this manual

 Read this operating manual completely before using the device for the first time. Please also note the

operating instructions for the accessories, if applicable.

 This operating manual is part of the product. Thus, it must always be easily accessible.

 Enclose this operating manual when transferring the device to third parties.

 If this manual is lost, please request another one. For the current version, please refer to our webpage

www.eppendorf.com

The Centrifuge 5430/5430 R is available in two versions: key pad or rotary knobs. This operating manual
generally describes how to operate the keypad version but it also applies to the rotary knob version.

(international) or www.eppendorfna.com (North America).

1.2 Danger symbols and danger levels

The safety instructions in this manual appear with the following danger symbols and danger levels:

7

1.2.1 Danger symbols

Biohazard Explosion

Electric shock Crushing

Hazard point Material damage

1.2.2 Danger levels

DANGER Will lead to serious injuries or death.

WARNING May lead to serious injuries or death.

CAUTION May lead to light to moderate injuries.

NOTICE May lead to material damage.

1.3 Symbols used

Example Meaning

 You are requested to perform an action.

1.

2.

• List.

Perform these actions in the sequence described.

Press this key to perform the described action.

Text Ter ms from the display of the device.

References useful information.

Operating instructions

Centrifuge 5430/5430 R

8

English (EN)

1.4 Abbreviations used

MTP

Micro test plate

PCR

Polymerase chain reaction

PTFE

Polytetrafluorethylene

RZB/rcf

Relative centrifugal force – g-force in m/s

rpm

Revolutions per minute – in rpm

2

UV

Ultraviolet radiation

2 Product description

1

2

3

4

5

678

1

2

3

4

5

1

9

6

8

7

2.1 Main illustration

Abb. 2-1: Front and rear view of Centrifuge 5430

Product description

Centrifuge 5430/5430 R

English (EN)

9

Fig. 2-1: Front and rear view of Centrifuge 5430

Abb. 2-2: Front and rear view of Centrifuge 5430 R

Fig. 2-2: Front and rear view of Centrifuge 5430 R

10

Product description

Centrifuge 5430/5430 R
English (EN)

1 Emergency lid release

On both sides of the device (see Emergency
release on p. 57).

2 Centrifuge lid

3 Control panel

Keys and dials (dependent on the device version)
for operating the centrifuge(see p. 28).

4Window

Visual control for rotor stop or option for speed
check via stroboscope.

5Display

Depiction of the current centrifuging parameters
and device settings (see p. 28).

6 Mains connection

Connection socket for the mains cable supplied.
Only 5430: The fuse holder is located beneath
(see Fuses on p. 53).

7 Mains switch

Switch for switching the device on and off.
Switch position 0: The device is switched off.
Switch position I: The device is switched on.

8ID plate

9USB port

Interface for error analysis and software updates
by the Technical Service.

2.2 Delivery package

2.2.1 Centrifuge 5430

Quantity Order no.

(International)

1– – See chapter Ordering Information for corresponding device

1 5301 850.249 022654403 4 A (230 V), 2 pieces
oder 5427 850.341 022654381 8.0 AT UL (120 V/100 V), 2 pieces

1 5416 301.001 022634305 Standard
1– – Mains power cable

1 5427 901.124 5427901124 Languages: EN, DE, FR, ES, IT, PT
1 5427 901.132 5427901132 Languages: DA, FI, EL, NL, SV (230 V devices only)

2.2.2 Centrifuge 5430 R

Quantity Order no.

(International)

1– – See chapter Ordering Information for corresponding device

1 5416 301.001 022634305 Standard
1– – Mains power cable

1 5427 901.124 5427901124 Languages: EN, DE, FR, ES, IT, PT
1 5427 901.132 5427901132 Languages: DA, FI, EL, NL, SV (230 V devices only)

Order no.
(North America)

Order no.
(North America)

Description

Centrifuge 5430

version, equipment and order number

Fuse

Rotor key

Operating manual Centrifuge 5430/5430 R

Description

Centrifuge 5430 R

version, equipment and order number

Rotor key

Operating manual Centrifuge 5430/5430 R

Product description

Centrifuge 5430/5430 R

English (EN)

2.3 Features

The multifaceted Centrifuge 5430 has a capacity of 48 × 2.0 mL and reaches max. 30,130 × g/17,500 min-1.
The versatility is reflected in the available rotor options. You can select from 12 different rotors to
centrifuge the following tubes for your various applications:

• Tubes (0.2 to 5.0 mL)

•PCR strips

• Microtainers

•Spin columns

•Cryo tubes

• Conical tubes (15/50 mL)

•Microplates

•PCR plates

• Deepwell plates (max. height 29 mm)

• Slides (with CombiSlide adapter)

Five program keys for rapid loading and saving of parameters, as well as another 45 program places, a
large display and menu-controlled operation all make it easier to use the centrifuge. The Centrifuge 5430
has been designed based on latest ergonomic studies. This facilitates an intuitive and easy operation.

11

The Centrifuge 5430 is available with two different operator panels: one easy to clean keypad or blue rotary
knobs to quickly set the centrifugation parameter.

The Centrifuge 5430 R has an additional temperature control function for centrifugation between -11°C and
+40°C. The Fast Temp function is used to start a temperature control run without samples in order to
quickly bring the rotor chamber, and rotor, bucket and adapter, to the set temperature. This temperature
control cycle can also be started automatically at specified times using the Fast Temp pro function.

12

Product description

Centrifuge 5430/5430 R
English (EN)

2.4 Rotors

The Centrifuge 5430/5430 R can be operated with the following rotors. Before using tubes, observe the
manufacturer's recommended specifications on resistance to centrifugation (max. g-force).

Rotor
FA-45-48-11

With aerosol-tight
QuickLock rotor lid

Rotor
F-45-48-11

With polypropylene
rotor lid

Max. capacity Max. g-force

(rcf)/speed
(rpm) without
adapter

Acceleration/
deceleration time
(soft): with soft ramp

48 tubes 1.5/2.0 mL.
With adapters:

• 0.2 mL PCR tubes

• 0.4 mL tubes

• 0.5 mL tubes

• 0.6 mL Microtainer

Outer ring:
18,213 × g
Inner ring:
16,048 × g/
12,700 rpm

≤ 20 s/20 s
≤ 61 s/65 s (soft)

48 tubes 1.5/2.0 mL.
With adapters:

• 0.2 mL PCR tubes

• 0.4 mL tubes

• 0.5 mL tubes

• 0.6 mL Microtainer

Outer ring:
18,213 × g
Inner ring:
16,048 × g/
12,700 rpm

≤ 20 s/20 s
≤ 61 s/65 s (soft)

Max.
load per
rotor

(1)

bore

(2)

3.75 g

3.75 g

Notes

• Aerosol-tight
QuickLock rotor lid
(aluminum).

(3)

Rotor
FA-45-30-11

With aerosol-tight
QuickLock rotor lid

Rotor
F-45-30-11

With polypropylene
rotor lid

30 tubes 1.5/2.0 mL.
With adapters:

• 0.2 mL PCR tubes

• 0.4 mL tubes

• 0.5 mL tubes

• 0.6 mL Microtainer

30 tubes 1.5/2.0 mL.
With adapters:

• 0.2 mL PCR tubes

• 0.4 mL tubes

• 0.5 mL tubes

• 0.6 mL Microtainer

20,817 × g/
14,000 rpm

≤ 15 s/15 s
≤ 61 s/65 s (soft)

20,817 × g/
14,000 rpm

≤ 15 s/15 s
≤ 61 s/65 s (soft)

3.75 g

3.75 g

• Aerosol-tight

(3)

QuickLock rotor lid
(aluminum).

•PTFE-coated
(particularly resistant
to chemicals),
marked: coated

•Spin columns
available, better with
rotor FA-45-24-11-kit.

Product description

Centrifuge 5430/5430 R

English (EN)

13

Rotor
FA-45-24-11-Kit

With aerosol-tight
QuickLock rotor lid

Rotor
FA-45-16-17

With aerosol-tight
QuickLock rotor lid

Max. capacity Max. g-force

(rcf)/speed
(rpm) without
adapter

Acceleration/
deceleration time
(soft): with soft ramp

24 spin columns or 1.5/2.0 mL
tubes.
With adapters:

• 0.2 mL PCR tubes

19.090 × g/
13,200 rpm

≤ 15 s/16 s
≤ 78 s/90 s (soft)

• 0.4 mL tubes

• 0.5 mL tubes

• 0.6 mL Microtainer

16 tubes 5.0 mL. 21,191 × g/

14,200 rpm

≤ 20 s/20 s
≤ 61 s/66 s (soft)

Max.
load per
rotor

(1)

bore

(2)

3.75 g

9.5 g

Notes

• Aerosol-tight

(3)

QuickLock rotor lid
(aluminum).

• Uniquely high edge,
for all commercial
spin columns.

Also observe the note
on centrifugation with
open tube lids (see

Fixed-angle rotors on
p. 33)

• Aerosol-tight

(3)

QuickLock rotor lid
(aluminum).

Rotor
S-24-11-AT

With aerosol-tight
QuickLock rotor lid

24 tubes 1.5/2.0 mL. 16,049 × g/

12,700 rpm

This rotor exclusively is to be
used with 1.5/2.0 mL reaction

≤ 13 s/16 s

≤ 61 s/66 s (soft)
vessels. Spin Columns and the
adapters including the
corresponding 0.2 mL, 0.4 mL,

0.5 mL and 0.6 mL vessels
must not be used in this rotor.

3.75 g

• Aerosol-tight

(3)

QuickLock rotor lid
(aluminum).

•The rotor must always
be operated with a
rotor lid.

14

Product description

Centrifuge 5430/5430 R
English (EN)

Rotor
FA-45-24-11-HS

Rotor
F-45-64-5-PCR

Max. capacity Max. g-force

(rcf)/speed
(rpm) without
adapter

Acceleration/
deceleration time
(soft): with soft ramp

24 tubes 1.5/2.0 mL.
With adapters:

• 0.2 mL PCR tubes

• 0.4 mL tubes

30,130 × g/
17,500 rpm

≤ 21 s/16 s
≤ 61 s/65 s (soft)

• 0.5 mL tubes

• 0.6 mL Microtainer

64 PCR tubes (0.2 mL)
or

13,543 × g/

11,800 rpm
eight 5-tube or 8-tube
PCR strips,
each with the enclosed

≤ 12 s/15 s

≤ 62 s/65 s (soft)
adapters.

Max.
load per
rotor

(1)

bore

(2)

3.75 g

3.4 g
(without
adapter)

Notes

• Aerosol-tight

(3)

rotor

lid (aluminum).

• Max. g-force/speed
(30,130 × g/17,500
rpm) only with tubes
approved by the man­ufacturer for this
speed.

• PTFE-coated (particu­larly resistant to
chemicals), marked:

coated

• Spin columns avail­able, better with rotor
FA-45-24-11-kit.

•The rotor can only be
tightened and loos­ened using the special
rotor key for rotor
FA-45-24-11-HS (see
p. 42).

Rotor
F-45-18-17-Cryo

18 Cryo tubes
or
18 sealable centrifugation
tubes,
max. Ø: 16.9 mm.
With supplied adapters: max.
Ø: 13.4 mm, max. tube length:
50 mm.

8,324 × g/
8,900 rpm

≤ 8 s/11 s
≤ 77 s/85 s (soft)

8.7 g • Setting the g-force/
speed in increments
of 10 × g or 10 rpm.

Product description

Fixed-angle

rotor

Aerosol-tight

version

Angle of

bores

Number of

bores

∅ of

bores (mm)

AT

24 11

Aerosol-tight

version

Number of

bores

∅ of

bores (mm)

Swing-bucket

rotor

Centrifuge 5430/5430 R

English (EN)

15

Rotor
F-35-6-30

Rotor
A-2-MTP

Max. capacity Max. g-force

(rcf)/speed
(rpm) without
adapter

Acceleration/
deceleration time
(soft): with soft ramp

6 conical tubes 50 mL, with or
without skirted bottom
or
6 conical tubes 15 mL, with

7,745 × g/
7,830 rpm

≤ 23 s/23 s
≤ 62 s/67 s (soft)

enclosed adapters
or
6 Centriplus centrifuge filter
units with adapters.

Two buckets to hold:

•Microplates

2,204 × g/
4,680 rpm

• Cell culture plates

•PCR plates

• Deepwell plates (max.

≤ 18 s/21 s
≤ 63 s/67 s (soft)

height 29 mm)

• Slide (with CombiSlide
adapter)

Max.

Notes
load per
rotor

(1)

bore

(2)

110 g • The rotor can only be

removed and inserted
using the supplied
removal tool.

•Centrifugation of
round-bottom tubes
and blood taking
systems can be
completed using
additional adapters
(see p. 86).

170 g
(per
bucket)

• PCR plates can only
be centrifuged with
appropriate adapters.

• Max. loading height:
29 mm.

• Only 5430 R: More
efficient cooling via
centrifugation without
wind shield upper
shell (see

Swing-bucket rotor on
p. 34).

(1) Maximum load per rotor bore for adapter + tube + contents.

(2) According to DIN 58 970 (device versions: 230 V, 120 V and 100 V, 50 to 60 Hz).

(3) Aerosol tightness tested and certified by the Centre of Emergency Preparedness and Response, Health
Protection Agency, Porton Down (UK) (see certificates at the end of this operating manual).

For the rotors and rotor lids labeled coated, color fluctuations may occur as a result of the production
process. These fluctuations have no effect on service life or resistance to chemicals.

16

Product description

Centrifuge 5430/5430 R
English (EN)

2.4.1 rcf display and calculation

Use the rpm/rcf key to switch the speed of centrifugation display from rpm to g-force
(rcf). Ensure that the g-force displayed during switching is standardized to suit the
rotor in question without an adapter. When adapters are used, the following maximum
g-forces (rcf) can be achieved at maximum speed:

Rotor Adapter Max.centrifugation

radius r

Rotor FA-45-48-11/
Rotor F-45-48-11

Rotor FA-45-30-11/
Rotor F-45-30-11

Rotor
FA-45-24-11-Kit

Rotor FA-45-16-17 For 5.0 mL micro test tubes 9.4 21,191

Rotor S-24-11-AT Without adapter 8.9 16,049

Rotor
FA-45-24-11-HS

Rotor
F-45-64-5-PCR

Rotor
F-45-18-17-Cryo

Without adapter Outer ring: 10.1

Inner ring: 8.9

For 0.2 mL PCR tubes Outer ring: 8

Inner ring: 6.8

For 0.4 mL tubes Outer ring: 10.1

Inner ring: 8.9

For 0.5 mL tubes Outer ring: 9

Inner ring: 7.8

For 0.6 mL Microtainer Outer ring: 10.1

Inner ring: 8.9

Without adapter 9.5 20,871

For 0.2 mL PCR tubes 7.4 16.215

For 0.4 mL tubes 9.5 20,871

For 0.5 mL tubes 8.4 18,407

For 0.6 mL Microtainer 9.5 20,817

Without adapter 9.8 19,090

For 0.2 mL PCR tubes 7.7 15,000

For 0.4 mL tubes 9.8 19,090

For 0.5 mL tubes 8.7 16,950

For 0.6 mL Microtainer 9.8 19,090

Without adapter 8.8 30,130

For 0.2 mL PCR tubes 6.7 22,940

For 0.4 mL tubes 8.8 30,130

For 0.5 mL tubes 7.7 26,364

For 0.6 mL Microtainer 8.8 30,130

For PCR strips, inside 7.7 11,987

For PCR strips, outside 8.7 13,543

Without adapter 9.4 8,320

For Cryo tubes 9.0 7,970

max

[cm]

Max. g-force (rcf)

Outer ring: 18,210
Inner ring: 16,048

Outer ring: 14,425
Inner ring: 12,261

Outer ring: 18,210
Inner ring: 16,048

Outer ring: 16,229
Inner ring: 14,065

Outer ring: 18,210
Inner ring: 16,048

Product description

Centrifuge 5430/5430 R

English (EN)

17

Rotor Adapter Max.centrifugation

radius r

max

[cm]

Max. g-force (rcf)

Rotor F-35-6-30* For 15 mL conical tubes 11.0 7,540

For 50 mL conical tubes 10.5 7,197

for Centriplus centrifuge filter units 11.1 7,567

A-2-MTP rotor Without adapter 9.0 2,204

for 384 well PCR plates 7.7 1,885

for 96 well PCR plates 7.3 1,788

CombiSlide adapter 7.7 1,885

*) Centrifugation of round-bottom tubes and blood taking systems can be done using additional adapters
(see p. 86).

To determine the g-force (rcf) for a special adapter, you can perform a calculation according to DIN 58 970
with the following formula:

-5

rcf = 1.118 · 10

· n2 · r

max

n: speed in min-1 (rpm)

: max. centrifuging radius in cm

r

max

Example:

In Rotor FA-45-30-11, the 0.5 mL adapter has a maximum radius of 8.4 cm. At 7,000 rpm, a maximum
g-force of 4,600 × g is achieved.

18

Safety

Centrifuge 5430/5430 R
English (EN)

3Safety

3.1 Intended use

The Centrifuge 5430/5430 R is intended exclusively for indoor use and for separating aqueous solutions
and suspensions of various densities in approved test tubes.

3.2 User profile

This device may only be operated by trained specialist staff. They must have carefully read the operating
manual and be familiar with the function of the device.

3.3 Application limits

3.3.1 Declaration concerning the ATEX directive (94/9/EC)

DANGER! Risk of explosion.

 Do not operate the device in areas where work is completed with explosive substances.
 Do not use this device to process any explosive or highly reactive substances.
 Do not use this device to process any substances which may generate an explosive

atmosphere.

Due to its design and the environmental conditions inside the device, the Centrifuge 5430/5430 R is not
suitable for use in a potentially explosive atmosphere.

The device only must be used in a safe environment, such as the open environment of a ventilated
laboratory or fume hood. The use of substances which could create a potentially explosive atmosphere is
not permitted. The final decision on the risks associated with the use of these types of substances is the
responsibility of the user.

3.3.2 Maximum service life for accessories

WARNING! Risk of injury from chemically or mechanically damaged accessories.

Even minor scratches and cracks can lead to serious internal material damage.

 Protect all accessory parts from mechanical damage.
 Inspect the accessories for damage before each use. Replace any damaged accessories.
 Do not use rotors, rotor lids or buckets with signs of corrosion or mechanical damage (e.g.,

deformations).

 Do not use any accessories whose maximum service life has been exceeded.
 When inserting the buckets and rotors, ensure that they do not become scratched.

CAUTION! Risk of injury due to chemically damaged rotor lids or caps.

Transparent rotor lids or caps made from PC, PP or PEI may lose their strength under the
impact of organic solvents (e.g., phenol, chloroform).

 If rotor lids or caps have come into contact with organic solvents, they should be cleaned

immediately.

 Regularly check the rotor lids and caps for damages and cracks.
 Immediately replace rotor lids or caps that have cracks or milky stains.

Safety

Centrifuge 5430/5430 R

English (EN)

The rotors listed below, and the corresponding buckets and rotor lids, have a maximum service life of the
number of years or cycles listed in the table.
Since 2012, Eppendorf has been stating the maximum service life of the rotors and accessories both in
years and in the maximum number of cycles. The decisive factor for the service life is which case occurs
first, generally this is the number of years in operation.

Rotor Maximum service from commissioning onward

FA-45-48-11
(With QuickLock)

F-45-48-11
(With rotor lid thread)

FA-45-30-11
(With QuickLock)

FA-45-24-11-Kit
(With QuickLock)

FA-45-16-17
(With QuickLock)

S-24-11-AT
(With QuickLock)

A-2-MTP
With the corresponding bucket and upper shell
of the wind shield

QuickLock rotor lid - 3 years

Rotor lid and caps made of polycarbonate (PC),
polypropylene (PP) or polyetherimide (PEI)

Plastic adapters - 1 year

100,000 mechanical cycles 10 years

100,000 mechanical cycles 10 years

100,000 mechanical cycles 10 years

100,000 mechanical cycles 10 years

100,000 mechanical cycles 10 years

100,000 mechanical cycles 10 years

100,000 mechanical cycles 7 years

-3 years

19

For all other rotors and rotor lids of this centrifuge there is no service life limit as long as the following
requirements are met:

•Proper use,

• Recommended maintenance

• Undamaged condition

The date of manufacture is stamped on the rotors in the format 03/10 (= March 2010) or on the inside of the

plastic rotor lids in the form of a clock .

To ensure aerosol tightness, the following applies:

• Replace aerosol-tight rotor lids and caps after 50 autoclaving cycles.

• Replace the seal of QuickLock rotor lids after 50 autoclaving cycles.

20

Safety

Centrifuge 5430/5430 R
English (EN)

3.4 Information on product liability

In the following cases, the designated protection of the device may be compromised. Liability for any
resulting property damage or personal injury is then transferred to the operator:

• The device is not used in accordance with the operating manual.

• The device is used outside of its intended use.

• The device is used with accessories or consumables which are not recommended by Eppendorf.

• The device is maintained or repaired by people not authorized by Eppendorf.

• The user makes unauthorized changes to the device.

3.5 Warnings for intended use

Read the operating manual and observe the following general safety instructions before using the
Centrifuge 5430/5430 R.

3.5.1 Personal injury or damage to the equipment

WARNING! Electric shock due to damage to device or mains cable.

 Only switch on the device if the device and mains cable are undamaged.
 Only use devices that have been properly installed or repaired.
 In case of danger, disconnect the device from the mains supply by pulling the power plug

from the device or the mains socket or, by using the isolating device intended for this
purpose (e.g. emergency stop switch in the laboratory).

WARNING! Lethal voltages inside the device.

 Ensure that the housing is always closed and undamaged so that no parts inside the device

can be contacted by accident.

 Do not remove the housing of the device.
 Do not allow any liquids to penetrate the inside of the housing.
 Do not allow the device to be opened by anyone except service personnel who have been

specifically authorized by Eppendorf.

WARNING! Risk from incorrect supply voltage

 Only connect the device to voltage sources which correspond to the electrical

requirements on the name plate.

 Only use sockets with a protective earth (PE) conductor and suitable power cable.

WARNING! Damage to health due to infectious liquids and pathogenic germs.

 When handling infectious liquids and pathogenic germs, observe the national regulations,

the biological security level of your laboratory, the material safety data sheets, and the
manufacturer's application notes.

 Use aerosol tight sealing systems for the centrifugation of these substances.
 When working with pathogenic germs belonging to a higher risk group, more than one

aerosol-tight bioseal must be used.

 Wear personal protective equipment.
 For full instructions regarding the handling of germs or biological material of risk group II

or higher, please refer to the "Laboratory Biosafety Manual" (Source: World Health
Organization, current edition of the Laboratory Biosafety Manual).

Safety

Centrifuge 5430/5430 R

English (EN)

WARNING! Risk of injury when opening or closing the centrifuge lid.

There is a risk of crushing your fingers when opening or closing the centrifuge lid.

 When opening or closing the centrifuge lid, do not reach between the lid and device or into

the latching mechanism of the lid.

 Always open the centrifuge lid completely to prevent it from falling.

CAUTION! Poor safety due to incorrect accessories and spare parts.

The use of accessories and spare parts other than those recommended by Eppendorf may
impair the safety, functioning and precision of the device. Eppendorf cannot be held liable or
accept any liability for damage resulting from the use of incorrect or non-recommended
accessories and spare parts, or from the improper use of such equipment.

 Only use accessories and original spare parts recommended by Eppendorf.

NOTICE! Damage to device due to spilled liquids.

1. Switch off the device.

2. Disconnect the device from the power supply.

3. Carefully clean the device and the accessories in accordance with the cleaning and
disinfection instructions in the operating manual.

4. If a different cleaning and disinfecting method is to be used, contact Eppendorf AG to
ensure that the intended method will not damage the device.

21

NOTICE! Damage to electronic components due to condensation.

Condensate can form in the device after it has been moved from a cool environment to a
warmer environment.

 After installing the device, wait for at least 4 h. Only then connect the device to the mains

power supply.

3.5.2 Incorrect handling of the centrifuge

NOTICE! Damage from knocking against or moving the device during operation.

If the rotor bangs against the rotor chamber wall, it will cause considerable damage to the
device and rotor.

 Do not move or knock against the device during operation.

22

Safety

Centrifuge 5430/5430 R
English (EN)

3.5.3 Incorrect handling of the rotors

WARNING! Risk of injury from improperly attached rotors and rotor lids.

 Only centrifuge with rotor and rotor lid firmly tightened.
 If unusual noises occur when the centrifuge starts, the rotor or the rotor lid may not be

properly secured. Immediately press the start/stop key to stop centrifuging.

CAUTION! Risk of injury due to asymmetric loading of a rotor.

 Load rotors symmetrically with identical tubes or plates and buckets.
 Only load adapters with suitable tubes or plates.
 Always use tubes or plates of the same type (weight, material/density and volume).
 Check that loading is symmetrical by balancing the adapters and tubes or plates used with

scales.
The device automatically detects imbalances during operation and stops the run immediately
with an error message and a signal tone. Check the loading, balance the tubes and re-start the
centrifugation.

CAUTION! Risk of injury from overloaded rotor.

The Centrifuge 5430/5430 R is designed for the centrifugation of material with a max. density
of 1.2 g/mL at maximum speed and filling volume and/or load.

 Do not exceed the maximum load of the rotor.

NOTICE! Damage to rotors from aggressive chemicals.

Rotors are high-quality components which withstand extreme stresses. This stability can be
impaired by aggressive chemicals.

 Avoid using aggressive chemicals, including strong and weak alkalis, strong acids,

solutions with mercury, copper and other heavy metal ions, halogenated hydrocarbons,

concentrated saline solutions and phenol.

 If the rotor is contaminated by aggressive chemicals, clean it immediately using a neutral

cleaning agent. This applies to the rotor bores, in particular.

 Due to the manufacturing process, color variations may occur on rotors marked "coated".

These color variations do not effect service life or resistance to chemicals.

NOTICE! If handled incorrectly, the rotor can fall over.

The rotor can fall over if the buckets are used as a handle.

 Remove the buckets before inserting and/or removing a swing-bucket rotor.
 Always use both hands to carry the rotor cross.

CAUTION! Risk of injury due to chemically damaged rotor lids or caps.

Transparent rotor lids or caps made from PC, PP or PEI may lose their strength under the
impact of organic solvents (e.g., phenol, chloroform).

 If rotor lids or caps have come into contact with organic solvents, they should be cleaned

immediately.

 Regularly check the rotor lids and caps for damages and cracks.
 Immediately replace rotor lids or caps that have cracks or milky stains.

3.5.4 Extreme strain on the centrifuging tubes

CAUTION! Risk of injury from overloaded tubes.

 Note the loading limits specified by the tube manufacturer.
 Only use tubes which are approved by the manufacturer for the required rcf.

NOTICE! Risk from damaged tubes.

Damaged tubes must not be used, as this could cause further damage to the device and the
accessories and loss of the samples.

 Before use, visually check all of the tubes for damage.

NOTICE! Risk from open tube lids.

Open tube lids can brake off during centrifugation and damage the rotor and the centrifuge.

 Carefully seal all tube lids before centrifuging.

Exception: Note the information on the centrifugation of spin columns in the rotor
FA-45-24-11-Kit (see p. 34).

Safety

Centrifuge 5430/5430 R

English (EN)

23

NOTICE! Hazard to plastic tubes from organic solvents.

The density of plastic tubes is reduced when organic solvents (e.g., phenol, chloroform) are
used, i.e. the tubes could become damaged.

 Observe the manufacturer's specifications for chemical resistance of the tubes.

NOTICE! Sample tubes heat up.

In uncooled centrifuges, the temperature in the rotor chamber, rotor and sample can increase
to above 40 °C, based on the run time, g-force (rcf)/speed and ambient temperature.

 Note that this can reduce the centrifugation resistance of the sample tubes.
 Please note the temperature resistance of the samples.

24

Safety

Centrifuge 5430/5430 R
English (EN)

3.5.5 Aerosol-tight centrifugation

WARNING! Risk to health due to limited aerosol tightness with incorrect rotor/rotor lid
combination.

Aerosol-tight centrifugation is guaranteed only if the rotors and rotor lids intended for this
purpose are used. The designation of aerosol-tight fixed-angle rotors always starts with
FA.The aerosol-tight rotors and rotor lids of this centrifuge are additionally marked with a red
ring on the rotor and a red rotor lid screw.
Aerosol-tight swing-bucket rotors are marked with AT (aerosol-tight).

 For aerosol-tight centrifugation, always simultaneously use rotors and rotor lids which are

marked as aerosol-tight in the centrifuge intended for the corresponding purpose. The

details specifying in which centrifuge you may use the aerosol-tight rotors and rotor lids

can be found on the rotor and, beginning from production date of October 2003, on the

upper side of the rotor lid.

 Only use aerosol-tight rotor lids in combination with rotors which are marked on the rotor

lid.

 Only use aerosol-tight buckets with the corresponding caps.

WARNING! Damage to health as a result of limited aerosol tightness and incorrect usage.

Autoclaving, mechanical stresses and contamination by chemicals or other aggressive
solvents can impair the aerosol-tightness of the rotors and rotor lids.

 Check the integrity of the seals of the aerosol-tight rotor lids or caps before each use.
 Only use aerosol-tight rotor lids or caps if the seals are undamaged and clean.
 Lightly grease the threads of the rotor lid screw with pivot grease after every proper

autoclaving (121 °C, 20 min.) (int. order no. Int. 5810 350.050, North America

022634330).

 Replace aerosol-tight rotor lids and caps after 50 autoclaving cycles.
 For QuickLock rotor lids, the seal must be replaced after 50 autoclaving cycles.
 Never store aerosol-tight rotors or buckets closed.

3.6 Safety notes on the device

Symbol Meaning Location

Hazard point. 5430: Rear of the device

CAUTION

Always tighten the rotor with the enclosed rotor
key.

CAUTION

Close all tubes and use a rotor lid.

5430 R: Right side of the
device

Upper side of device, under
the centrifuge lid.

Upper side of device, under
the centrifuge lid.

4 Installation

4.1 Selecting the location

NOTICE! If an error occurs, the objects in the immediate proximity of the device will be
damaged.

 In accordance with recommendations in EN 61010-2-020, leave a safety clearance of

30 cm around the device during operation.

 Please remove all materials and objects from this area.

NOTICE! Damage from overheating.

 Do not install the device near heat sources (e.g. heating, drying cabinet).
 Do not expose the device to direct sunlight.
 Ensure unobstructed air circulation. Maintain a clearance of at least 30 cm around all

ventilation grilles.

Installation

Centrifuge 5430/5430 R

English (EN)

25

Select the location for the device according to the following criteria:

• Suitable power connection as per the name plate (230 V/120 V/100 V).

• Stable, horizontal and resonance-free lab bench.

• A well ventilated environment which is protected from direct sunlight to prevent the device from heating
up more.

4.2 Preparing installation

Prerequisites

The weight of the centrifuge is 29 kg (5430) or 56 kg (5430 R). A second person is needed to unpack and
position the device.

Retain the packaging material and the transport protection device for subsequent transport or
storage. See also the instructions relating to transport (see p. 58).

 Perform the following steps in the sequence described.

Centrifuge 5430 Centrifuge 5430 R

1. Open the packaging board.

2. Remove the covering cardboard.

3. Remove the accessories.

4. Grip from the strap retainers and have two
persons lift the centrifuge out of the box.

5. Pull off the strap retainers, do not cut.

6. Remove the front and rear transport securing
devices from the centrifuge.

7. Remove the plastic sleeve.

8. Carefully lift the centrifuge on one side and pull
off the transport securing device of the motor
on the underside of the centrifuge.

1. Open the packaging board.

2. Remove the accessories.

3. Lift and remove the front and rear transport
securing devices.

4. Grip from the fabric straps and have two people
lift the centrifuge out of the box.

5. Pull off the textile straps, do not cut.

6. Remove the plastic sleeve.

26

Installation

Centrifuge 5430/5430 R
English (EN)

4.3 Installing the instrument

Prerequisites

The device is on a suitable lab bench.

NOTICE! Damage to electronic components due to condensation.

Condensate can form in the device after it has been moved from a cool environment to a
warmer environment.

 After installing the device, wait for at least 4 h. Only then connect the device to the mains

power supply.

NOTICE! Centrifuge 5430 R: compressor damage after improper transport.

 Only switch on the centrifuge 4 h after installation.

Perform the following steps in the sequence described.

1. Let the device warm up to ambient temperature for at least 4 hours to avoid damaging electronic
assemblies as a result of condensation and avoid damaging the compressor (only 5430 R).

2. Check that the mains voltage and frequency match the requirements on the device type plate.

3. Connect the centrifuge to the mains and switch it on using the mains/power switch on the rear of the
device (Centrifuge 5430) or at the right side of the device (Centrifuge 5430 R).

• The standby key lights green.

•The display is active.

• Only 5430: Lid opens automatically

4. Only 5430: Remove the transport securing device of the motor shaft.

5. If the scope of delivery includes a rotor, dismantle and remove it using the supplied rotor key.

6. Only 5430: Remove the transport securing device of the air guide ring.

Centrifuge 5430/5430 R

7. Use the details included in the scope of delivery to check that the delivery is complete.

8. Check all parts for any transport damage. Contact your dealer if any damage is found.

Retain the packaging material and the transport protection device for subsequent transport or
storage. See also the instructions relating to transport (see p. 58).

Installation

27

English (EN)

28

1

2

3

4

5

6

7891011

13
12

Operation

Centrifuge 5430/5430 R
English (EN)

5 Operation

5.1 Overview of operating controls

The Centrifuge 5430/5430 R is available in two versions: keypad or rotary knobs. This operating manual
generally describes how to operate the keypad version. However, it also applies to the rotary knob version.

Before using the Centrifuge 5430/5430 R for the first time, familiarize yourself with the operating controls
and the display.

Abb. 5-1: Control panel and display of Centrifuge 5430/5430 R (keypad version).

Fig. 5-1: Control panel and display of Centrifuge 5430/5430 R (keypad version).

1 Select program

Press briefly: load the stored centrifuging

parameters.
Press and hold (> 2 sec): save the current
centrifuging parameters (see p. 47).

2 Short Spin centrifugation

(see p. 40)

3Release lid

4 Start and stop centrifugation

5 Activate/deactivate standby mode

Key lights green: centrifuge is ready for
operation.
Key lights red: standby mode active (see p. 41).

6Display

8 Adjust the temperature (only 5430 R)

9 Adjust the centrifuging duration

Dependent on device version, designed as key or
rotary knob.

10 Start the temperature control cycle Fast Temp

(only 5430 R)

(see p. 36)

11 Switch the displayed centrifuging speed (rpm/

rcf)

12 Call and select the menu parameters

(see p. 47)

13 Navigating the menu

(see p. 29)

7 Set speed of centrifugation

Dependent on device version, designed as key or
rotary knob.

Abb. 5-2: Display of the Ce ntrifuge 5430/5430 R

1

2 3 4 5 7

6

8

10

9

111213

Fig. 5-2: Display of the Centrifuge 5430/5430 R

Operation

Centrifuge 5430/5430 R

English (EN)

29

1 Program number (if enabled)

2 Status of the function At set rpm

: Start of operation when reaching 95% of the

preset g-force (rcf) or speed.

: Start of run time immediately.

3 Status of the key lock

: Centrifuging parameters cannot be modified

unintentionally.

: No key lock.

4 Status of the loudspeaker

: Switched on.

: Switched off.

5 Status of the centrifuge

: Centrifuge lid unlocked.

: Centrifuge lid locked.

(flashing): Centrifuging in progress.

6 Temperature control cycle programming (only

5430 R)

: Fast Temp pro is enabled, i.e., start time
and temperature of the temperature control run
have been programmed (see p. 37).

8Soft ramp

: Rotor accelerates and brakes slowly.

No symbol: Rotor accelerates and brakes rapidly.

9Standard display

10 Extended display (if enabled)

11 g-force/speed

12 Temperature (only 5430 R)

13 Centrifuging duration

7Time

Please also read the precise description of the individual functions (see p. 47).

5.2 Menu navigation

The menu consists of two levels. To change settings, generally proceed as follows.

1. Open menu.

30

Operation

Centrifuge 5430/5430 R
English (EN)

2. Select the desired menu item.

3. Confirm selection.

4. Select the setting of the parameters in question.

5.

6.

Confirm changed setting.
A tick appears in front of the confirmed setting.

Keep pressing the key until you reach the desired menu level or exit the menu.
Some menus can only be exited by selecting and confirming the menu item Back /

Zurück / Retour / Atrás.

5.3 Configure centrifuge

5.3.1 Set menu language

Proceed as follows to set menu language.

1. Open the menu.

2. Select Settings.

3. Confirm your selection.

4. Select Language.

5. Confirm your selection.

6. Select English, Deutsch, Francais or Espanol.

7.

8. Press key several times to exit the menu.

Confirm your selection. A tick appears in front of the selected language. The setting
takes effect immediately.

5.3.2 Setting the date and time

Proceed as follows to set date and time.

1. Open the menu.

2. Select Settings.

3. Confirm your selection.

4. Select Date/time.

5. Confirm your selection.

6. Set date.

7. Confirm setting.

Operation

Centrifuge 5430/5430 R

English (EN)

31

8. Set time format (12 h/24 h).

9. Confirm setting.

10. Set time.

11. Confirm setting.

12. Press key several times to exit the menu.

There is no automatic switch between summer time and winter time.

32

Operation

Centrifuge 5430/5430 R
English (EN)

5.4 Preparing for centrifugation

5.4.1 Switching on the centrifuge

1. Switch on the centrifuge using the mains power switch or the standby key.

Only 5430: After switching on at the mains power switch, the centrifuge lid opens automatically.

2. Open the closed centrifuge lid by pressing the open key.

The parameter settings of the last run are displayed.

5.4.2 Inserting the rotor

 Swing-bucket rotors: remove the buckets before inserting and/or removing the rotor. Use

both hands to pick up the rotor cross.

 Rotor F-35-6-30: only use the rotor removal tool supplied to insert or remove the rotor.

1. Fit the rotor vertically on the motor shaft.

2. Insert the supplied rotor key into the rotor nut.

Rotor FA-45-24-11-HS: Use the special rotor key.

3. Turn rotor key clockwise until the rotor nut is firmly tightened.

5.4.3 Automatic rotor detection

The centrifuge has automatic rotor detection. It detects a newly inserted rotor during centrifugation and
displays its name for approx. 2 seconds. The set g-force (rcf)/speed (rpm) is automatically limited to the
maximum permissible value of the rotor, if necessary.

If you start centrifuging immediately after a rotor change, the centrifuge has not carried out
an automatic rotor detection yet. The speed set for the previous rotor may exceed the
maximum permitted speed for the new rotor. In this case the centrifuge stops after the
automatic rotor detection and displays the error message Note C. The new maximum
permitted speed appears in the display. You can then restart the centrifuging with this setting
or adjust the speed as necessary.

 Always check the set g-force (rcf)/speed (rpm) after a rotor change and adjust it if necessary.

5.4.4 Manual rotor detection

CAUTION! Risk of injury when turning the rotor manually.

 When turning a swing-bucket rotor, pay special attention to ensure that your fingers do not

get jammed or get caught on the swinging buckets.

 In order to trigger the rotor detection manually, turn the rotor counterclockwise by hand.

• The name of the rotor appears in the display.

• The g-force (rcf)/speed (rpm) automatically is limited to the maximum value of the rotor.

5.4.5 Loading the rotor

CAUTION! Risk of injury due to asymmetric loading of a rotor.

 Load rotors symmetrically with identical tubes or plates and buckets.
 Only load adapters with suitable tubes or plates.
 Always use tubes or plates of the same type (weight, material/density and volume).
 Check that loading is symmetrical by balancing the adapters and tubes or plates used with

scales.
The device automatically detects imbalances during operation and stops the run immediately
with an error message and a signal tone. Check the loading, balance the tubes and re-start the
centrifugation.

CAUTION! Risk from damaged or overloaded tubes.

 When loading the rotor, observe the safety precautions on dangers as a result of

overloaded or damaged tubes (see Warnings for intended use on p. 20).

Operation

Centrifuge 5430/5430 R

English (EN)

33

The device automatically detects imbalances during operation and stops the run immediately
with an error message and a signal tone.

 Check the load, balance the tubes and restart the run.

5.4.5.1 Fixed-angle rotors

Rotor lid

• Fixed-angle rotors may only be operated with the appropriate rotor lid in each case. This is
clearly shown by the identical rotor name labeling on the rotor and on the rotor lid.

• To carry out an aerosol-tight centrifugation, an aerosol-tight rotor (label: red ring) and the
corresponding aerosol-tight rotor lid (label: aerosol-tight and red lid screw) must be used.

To load the rotor, proceed as follows:

1. Check the maximum load (adapter, tube and contents) per rotor bore.

The information about this can be found on the rotor and in this operating manual (see Rotors on p. 12).

2. Load rotors and adapters only with the tubes intended for them.

3. Insert tubes opposite each other in pairs into the rotor bores. To ensure symmetric loading, tubes that
are arranged opposite each other must be of the same type and contain the same filling quantity.

To minimize weight differences between filled sample tubes, we recommend taring with a scale. This
will reduce wear on the drive and reduce running noise.

4. Attach and tighten rotor lid.

34

Operation

Centrifuge 5430/5430 R
English (EN)

Spin columns

For centrifuging spin columns in the rotor FA-45-24-11-Kit, you can leave the tube lids open.
However, this can only be done using the tubes provided by kit manufacturers for this
purpose. For reliable centrifugation, you must lean the open tube lids against the edge of the
rotor. Ensure that this does not involve the lids projecting beyond the edge of the rotor, and
then put on the associated rotor lid.

5.4.5.2 Swing-bucket rotor

Prerequisites

• A combination of rotor, carrier and adapter, approved by Eppendorf.

• Matching and tested tubes and plates.

NOTICE! Filling the plates too high can cause overflowing.

During the run the meniscuses in the tubes along the edges of the plates are at an angle. This
is due to the centrifugal forces and cannot be avoided.

 Fill the wells of the plates to a maximum of 2/3 of the max. capacity.

To load the rotor, proceed as follows:

1. Check the carrier grooves for cleanliness and grease lightly with pivot grease 
(order no. Int.: 5810 350.050 / North America: 022634330).

Dirty grooves and pivots prevent carriers from swinging out evenly.

2. Hang the buckets into the rotor.

All rotor positions must be loaded with carriers.

3. Check that all carriers are hanging properly and can swing freely.

4. Carry out a manual loading and swing test the first time a tube or plate type is used.

5. Check the maximum load per carrier (adapter, tube or plate and contents) and the loading height.

The information about this can be found on the rotor and in this operating manual (see Rotors on p. 12).

Operation

Centrifuge 5430/5430 R

English (EN)

6. Load the buckets symmetrically.

Abb. 5-3: Incomplete, but symmetric loading of the buckets. The pegs of each bucket must be uniformly loaded .

Fig. 5-3: Incomplete, but symmetric loading of the buckets. The pegs of each bucket must be uniformly

loaded.

Abb. 5-4: Symmetrical loa ding of the plates.

35

Fig. 5-4: Symmetrical loading of the plates.

The plate arrangement shown on the right-hand side is incorrect, as the buckets will not swing properly.

The plates have some play in the buckets.

7. Check the loading of the bucket.

 Carry out a brief centrifugation test at low speed (e.g., 1000 rpm) when you use a tube or

plate type for the first time.

Only 5430 R: When using the A-2-MTPs, centrifuge without the upper shell of the wind shield
in order to guarantee precise and quick temperature control of samples. Note that the
centrifugation noise will increase slightly in this case.
This does not apply to the S-24-11-AT. The S-24-11-AT must always be operated with a rotor
lid.

5.4.6 Closing the centrifuge lid

WARNING! Risk of injury when opening or closing the centrifuge lid.

There is a risk of crushing your fingers when opening or closing the centrifuge lid.

 When opening or closing the centrifuge lid, do not reach between the lid and device or into

the latching mechanism of the lid.

 Always open the centrifuge lid completely to prevent it from falling.

1. Check the correct attachment of the rotor and rotor lid.

2. Push down the centrifuge lid until the lid latch engages and the lid is automatically closed.

The centrifuge will close automatically.

The open key lights up blue. The symbol appears in the display.

36

Operation

Centrifuge 5430/5430 R
English (EN)

5.5 Cooling (only 5430 R)

5.5.1 Temperature adjustment

 Set the temperature using the temp arrow keys between -11°C and +40°C.

You can also modify the temperature during centrifugation.

At higher ambient temperature a brief fan noise is possible until the desired temperature has been
reached. This indicates a heavy cooling performance.

5.5.2 Temperature display

If the rotor is stopped: Target temperature
During centrifugation: Actual temperature

The set temperature appears in the extended display.

5.5.3 Temperature monitoring

After the target temperature has been reached, the centrifuge reacts to temperature deviations during
centrifugation as follows:

Deviation from set value Action

± 3 °C Temperatures on the display are flashing.

± 5 °C Periodic warning tone and display Error 18. Centrifugation is stopped

automatically.

5.5.4 FastTemp

This function can be used to start a temperature control run directly without samples with a rotor and
temperature-specific speed in order to quickly adjust the rotor chamber, including the rotor, buckets and
adapters, to the previously set nominal temperature.

The FastTemp pro function for programming the temperature control run with defined start times is
described in the next section.

Prerequisites

• The centrifuge is switched on.

• The rotor and rotor lid are properly attached.

• The centrifuge lid is closed.

• Temperature and g-force (rcf)/speed (rpm) for the centrifugation are set (see Centrifuging on p. 39).

1. Press the fast temp key.

The display shows Fast Temp, the remaining duration of the temperature control run as well as the
current temperature and g-force (rcf)/speed (rpm).

The temperature control run automatically ends when the set temperature is reached. A periodic signal
tone sounds.

2. Press the start/stop key to terminate the temperature control run early.

After the set temperature has been reached and the temperature control run is complete, the centrifuge
keeps the rotor chamber with the centrifuge lid closed at the set target temperature if the temperature is
below the ambient temperature. However, independent of the target temperature, 4 °C must be met via
this continuous cooling in order to prevent the rotor chamber from freezing.

Operation

Centrifuge 5430/5430 R

English (EN)

The centrifuge stops the cycle automatically if the rotor or the buckets have reached the set
temperature. Therefore, there may be a delay of approx. 30 min between the display of the set
temperature and the automatic end of the temperature control run.

Only 5430 R: When using the A-2-MTPs, centrifuge without the upper shell of the wind shield
in order to guarantee precise and quick temperature control of samples. Note that the
centrifugation noise will increase slightly in this case.
This does not apply to the S-24-11-AT. The S-24-11-AT must always be operated with a rotor
lid.

5.5.5 FastTemp pro

You can have the previously described temperature control run FastTemp (see p. 36) start automatically at
a specified time. Two options are available:

Once The temperature control cycle is started once at the set time.

Repeatedly The temperature control cycle is started at the set time on the next specified weekday.

This is repeated for an unlimited period of time with each weekday specified.

37

5.5.5.1 Programming the start time

1. Select Fast Temp pro in the device menu.

2. Select Once or Repeatedly.

This selection only appears as long as the FastTemp pro function has not already been activated.
Otherwise it is only possible to edit or delete the programmed start time.

3. Only with Repeatedly: Activate/deactivate weekdays with menu/enter, select Continue and confirm with
menu/enter.

4. Enter date and time for the one-time or repeated start of the temperature control run as well as the set
temperature and confirm with menu/enter.

An overview of the current settings is displayed.

5. Edit the settings again or save.

6. Exit the menu.

• FastTemp pro is now activated. In the display the symbol appears as long as an automatic start

of a temperature control run is still outstanding. In the standby mode Fast Temp pro is displayed.

• The temperature control run FastTemp (see p. 36) starts automatically at the set time.

• After a one-off programmed temperature control run, the following symbol is extinguished . With

several programmed temperature control runs, the FastTemp pro remains active until you deactivate it.
To do this, select Fast Temp pro in the device menu and delete the settings.

5.5.5.2 Preparing the centrifuge

 Ensure that the centrifuge is switched on or in the standby mode during the start time set and the rotor

and rotor lid are properly attached and the centrifuge lid is closed.

5.5.5.3 Automatic start of the temperature control cycle

1. If the centrifuge is in standby mode, it switches to the operating mode 1 min before the set start time.

2. At the start time the temperature control run FastTemp (see FastTemp on p. 36) begins. Fast Temp pro

appears in the display.

Automatically starting the temperature control cycle is not possible during centrifugation.

38

Operation

Centrifuge 5430/5430 R
English (EN)

5.5.6 Continuous cooling

If the rotor stops, the rotor chamber will be maintained at the target temperature if the following
requirements have been met:

• The centrifuge is switched on.

• The centrifuge lid is closed.

• The target temperature is lower than the ambient temperature.

• The centrifuge is not in standby mode.

During continuous cooling the following applies:

• The set and actual temperature are displayed alternately.

• Irrespective of the set temperature, the temperature does not go below 4 °C to prevent the rotor
chamber from freezing and from increased condensation in the device.

• The temperature adjustment is slower because the rotor does not rotate during this process.

To end continuous cooling, open the centrifuge lid or press the standby key.

If the centrifuge is not used for more than 8 hours, the continuous cooling is switched off automatically.
The device then switches to standby mode. This protects against ice formation in the rotor chamber and
increased condensation in the device. With FastTemp you can quickly reach the desired temperature again
(see p. 36).

You can change the continuous cooling to endless operation at your own risk. To do so, in the device menu
under Continuous cooling enable the ∞ (see p. 48) item.

Operation

Centrifuge 5430/5430 R

English (EN)

5.6 Centrifuging

CAUTION! Risk from incorrectly-loaded rotors and damaged/overloaded tubes!

 Before commencing centrifugation, follow the safety instructions relating to risks from

asymmetrically loaded and/or overloaded rotors and from overloaded, damaged and/or
open tubes (see Warnings for intended use on p. 20).

WARNING! Risk of injury from improperly attached rotors and rotor lids.

 Only centrifuge with rotor and rotor lid firmly tightened.
 If unusual noises occur when the centrifuge starts, the rotor or the rotor lid may not be

properly secured. Immediately press the start/stop key to stop centrifuging.

Each of the centrifuging variants described here must be preceded by the preparation described above (see
Preparing for centrifugation on p. 32).

39

Only 5430 R: Please also note the instructions on cooling (see Cooling (only 5430 R) on p. 36).

5.6.1 Centrifugation with time setting

Perform the following steps in the sequence described.

1. Use the time arrow keys to set run time.

2. Only 5430 R: Use the temp arrow keys to adjust the temperature.

3. Use the speed arrow keys to the g-force (rcf)/speed.

4. Press start/stop to start centrifuging.

During centrifugation:

• blinks in the display as long as the rotor is running.

• The remaining run time will be displayed in minutes. The last minute is counted down in seconds.

• Only 5430 R: The current temperature will be displayed.

• The current g-force (rcf) or rotor speed is displayed.

• The shortcut keys, the , open and short keys, and all menu items which directly affect

centrifugation, are blocked during centrifugation.

End of centrifugation

• The centrifuge automatically stops after the set time has elapsed. The elapsed centrifugation will be

shown in a blinking display during the braking process. A signal tone sounds when the rotor is at a
standstill.

• Only 5430: The centrifuge lid opens automatically. The display shows the symbol .

• Only 5430 R: The centrifuge lid remains closed to maintain the sample temperature. It can be

opened lid by pressing the blinking open key.

5. Remove the material for centrifuging.

• During the run you can modify the total run time, the temperature (only 5430 R), the speed
and the rpm/rcf indication. The new parameters are adopted immediately. Please note that
the shortest new total run time that can be set is the elapsed time plus 2 minuts.

• You can also terminate the centrifugation before the set run time has elapsed by pressing
the start/stop key.

40

Operation

Centrifuge 5430/5430 R
English (EN)

5.6.2 Centrifuging in continuous operation

Perform the following steps in the sequence described.

1. Use the time arrow keys to set the continuous run.

The continuous operation function can be set above 99:59 h or below 30 seconds. The timer shows
to indicate continuous operation.

2. Only 5430 R: Use the temp arrow keys to adjust the temperature.

3. Use the speed arrow keys to the g-force (rcf)/speed.

4. Press start/stop to start centrifuging.

blinks in the display as long as the rotor is running.
Time is counted upwards, first in 30-second increments and then in minute increments from ten
minutes.

5. Press start/stop to end centrifuging after the desired time period.

• The centrifugation time will be shown in a blinking display during the braking process.

• A signal tone sounds when the rotor is at a standstill.

• Only 5430: The centrifuge lid opens automatically. The display shows the symbol .

• Only 5430 R: The centrifuge lid remains closed to maintain the sample temperature. It can be
opened lid by pressing the blinking open key.

6. Remove the material for centrifuging.

5.6.3 Short-spin centrifugation

You can carry out a short-spin cycle with the currently set or with the maximum g-force (rcf)/speed of the
rotor used. This is set in the device menu (see Other menu items on p. 48) before executing the following
steps in the sequence specified:

1. For short-spin cycle with the current g-force (rcf)/speed, set this directly with the arrow keys speed.

2. Only 5430 R: Use the temp arrow keys to adjust the temperature.

3. Start the short run: Hold the short key pressed down.

• blinks in the display as long as the rotor is running.

• The time is counted upwards in seconds.

• During short run centrifuging all other keys are blocked. However, short run centrifuging is
interrupted if another key is pressed simultaneously.

4. End short spin run: Release the short key.

• The centrifugation time will be shown in a blinking display during the braking process.

• A signal tone sounds when the rotor is at a standstill.

• Only 5430: The centrifuge lid opens automatically. The display shows the symbol .

• Only 5430 R: The centrifuge lid remains closed to maintain the sample temperature. It can be
opened lid by pressing the blinking open key.

5. Remove the material for centrifuging.

During the braking process, you can restart the centrifugation up to two times by pressing the
short key again.

The soft ramp does not work during short-spin centrifugation.

Operation

Centrifuge 5430/5430 R

English (EN)

5.6.4 Removing the rotor

 Swing-bucket rotors: remove the buckets before inserting and/or removing the rotor. Use

both hands to pick up the rotor cross.

 Rotor F-35-6-30: only use the rotor removal tool supplied to insert or remove the rotor.

1. Turn the rotor nut counterclockwise using the supplied rotor key.

Rotor FA-45-24-11-HS: use special rotor key.

2. Remove the rotor vertically in an upward motion.

3. Only 5430 R: Switch off the centrifuge after use and empty the condensation water tray (remove it

from the left side of the device). Leave centrifuge lid fully opened and protect it against closing.

5.7 Standby mode

The centrifuge automatically changes from the ready state to the standby mode if the following
prerequisites are met:

• The centrifuge was not used for the time set in the device menu (1 to 60 min)(see Settings on p. 49)

• Only 5430 R: The centrifuge lid is open.

41

In the Standby mode, the following applies :

• The standby key lights red.

• Only 5430 R: The rotor chamber is not cooled (see Continuous cooling on p. 38).

In the Ready state, the following applies:

• The centrifugation parameters are displayed.

• The standby key lights green.

• Only 5430 R: The rotor chamber is cooled when the centrifuge lid is closed (see Continuous cooling on

p. 38).

You can switch between standby mode and ready state at any time when centrifugation is not performed by
pressing the standby key.

42

1. 2.

1. 2.

Operation

Centrifuge 5430/5430 R
English (EN)

5.8 User instructions on rotors

5.8.1 Rotor F-35-6-30: rotor removal tool

5.8.1.1 Inserting the rotor

Prerequisites

The rotor nut is loose.

Use the rotor removal tool to insert the rotor in the centrifuge and remove it again.

1. Position the rotor removal tool with the narrow side on the rotor thread.

2. Tighten the rotor removal tool clockwise with approx. 3 revolutions.

3. Grip the rotor removal tool and insert the rotor.

4. Loosen and remove the rotor removal tool by turning it counterclockwise.

5. Inserting the rotor: Tighten the rotor using the enclosed rotor key (see Inserting the rotor on p. 32).

5.8.1.2 Loosening the rotor lid

Use the rotor removal tool to loosen a firmly tightened rotor lid screw.

1. Position the rotor removal tool with the wide side on the rotor lid screw.

2. Loosen the rotor lid screw by turning the rotor removal tool counterclockwise.

Operation

Centrifuge 5430/5430 R

English (EN)

5.8.2 Rotor A-2-MTP

5.8.2.1 Inserting the rotor

NOTICE! If handled incorrectly, the rotor can fall over.

The rotor can fall over if the buckets are used as a handle.

 Remove the buckets before inserting and/or removing a swing-bucket rotor.
 Always use both hands to carry the rotor cross.

5.8.2.2 Position and remove the upper shell of the wind shield

The upper shell of the wind shield is used for noise reduction.

1. Before it is used for the first time, remove the attachment that is mounted on the knob of the upper
shell of the wind shield .

2. Position the upper shell of the wind shield and rotate a max. 1/4 revolution until it is lowered onto the
rotor hub.

The lock indicator must protrude so far over the knob that the red marking is clearly visible:

43

The loose positioning of the upper shell is intentional and aids in self-centering.

3. Pull on the knob of the upper shell of the wind shield in order to lift it.

Only 5430 R: When using the A-2-MTPs, centrifuge without the upper shell of the wind shield
in order to guarantee precise and quck temperature control of samples. Note that the
centrifugation noise will increase slightly in this case.
This does not apply to the S-24-11-AT. The S-24-11-AT must always be operated with a rotor
lid.

5.8.3 Rotor FA-45-24-11-HS: using the special rotor key

5.8.3.1 Tightening the rotor

1. Insert the rotor key for rotor FA-45-24-11-HS in the rotor nut.

2. Turn the rotor key clockwise until it spins through ("click").

The rotor is correctly tightened.

5.8.3.2 Loosening the rotor

 Turn the rotor nut and rotor key for Rotor FA-45-24-11-HS counterclockwise.

The rotor key for Rotor FA-45-24-11-HS can only be used to tighten and loosen this rotor. For
the other rotors described in this operating manual, use the rotor key that is delivered with the
Centrifuge 5430/5430 R.

44

Operation

Centrifuge 5430/5430 R
English (EN)

5.8.4 QuickLock

Aerosol-tight rotors FA-45-48-11, FA-45-30-11, FA-45-24-11-Kit, FA-45-16-17 and S-24-11-AT have a quick
lock for the rotor lid (QuickLock).

Rotors FA-45-30-11 and FA-45-24-11-Kit have been redesigned and are now only available with a quick
lock (QuickLock) instead of a rotor lid thread.

Closing the rotor lid

1. Check the correct positioning of the external sealing ring in the groove.

2. Place the rotor lid on the rotor in a vertical motion.

3. To lock the rotor, turn the red rotor lid screw clockwise as far as it will go, and after an audible "click" is
heard.

The rotor is correctly locked after the audible "click" is heard!

If the locking system is stiff, lightly lubricate the pins in the rotor lid screw with pivot grease.

Operating controls and function

Centrifuge 5430/5430 R

English (EN)

6 Operating controls and function

6.1 Device menu

Most menu levels contain the additional menu item Back / Zurück / Retour / Atrás. Detailed information on
the menu can be found in the following chapters.

Tab. 6-1: Menu structure of the Centrifuge 5430/5430 R in four different menu languages.

English Deutsch Français Español Display

Programs

• Load program

•Save program

• Delete program

Soft ramp

•On

•Off

Key lock

•On

•Off

Programme

•Programm laden

•Programm
speichern

•Programm löschen

Softrampe

•An

•Aus

Tastensperre

•An

•Aus

Programmes

•Charger prog.

• Enregistrer prog.

• Supprimer prog.

Rampe douce

•Marche

•Arrêt

Verrouilla. de touches

•Marche

•Arrêt

Programas

•Cargar programa

•Guardar programa

• Borrar programa

Rampa suave

• Encendido

•Apagado

Bloqueo del teclado

• Encendido

•Apagado

45

At set rpm

•On

•Off

Short spin

• Maximum speed

•Current speed

Continuous cooling
(5430 R)

•8 h

•∞

Fast Temp pro
(5430 R)

•One-time-use

• Repeated use

Settings

•Display
–S

andard display

t

– Extended display

•Alarm
–On
–Off

At set rpm

•An

•Aus

Short Spin

• Maximaler Wert

• Aktueller Wert

Dauerkühlung
(5430 R)

•8 h

•∞

Fast Temp pro
(5430 R)

•Einmal

•Mehrmals

Einstellungen

•Anzeige
–Standardanzeige
– Erweiterte

Anzeige

•Lautsprecher
–An
–Aus

At set rpm

•Marche

•Arrêt

Short Spin

• Vitesse max

• Vitesse actuelle

Refrigération
continue (5430 R)

•8 h

•∞

Fast Temp pro
(5430 R)

•Une fois

•Plusieurs fois

Réglages

•Affichage
–Affichage

standard

–Affichage large

•Signal sonore
–Marche
–Arrêt

At set rpm

• Encendido

•Apagado

Short Spin

• Velocidad máximo

• Velocidad actual

Refrigeración
continua (5430 R)

•8 h

•∞

Fast Temp pro
(5430 R)

•Una vez

• Varias veces

Ajustes

•Indicador
–Indicador

estándar

–Indicador

extendido

•Altavoz
– Encendido
–Apagado

46

Operating controls and function

Centrifuge 5430/5430 R
English (EN)

English Deutsch Français Español Display

•Volume
–Cancel
–Save
–Default

• Date/Time • Datum/Uhrzeit • Date/Heure • Fecha/Hora

• Contrast
–Cancel
–Save
–Default

• Language
–English
–Deutsch
–Français
–Español

•Standby
–On
–Off
–Set time

•Lid release 

(5430)
–Automatic
–Manual

• Lautstärke
–Abbrechen
–Speichern
–Lieferzustand

•Kontrast
–Abbrechen
–Speichern
–Lieferzustand

•Sprache
–English
–Deutsch
–Français
–Español

•Standby
–An
–Aus
– Zeit einstellen

•Deckelöffnung

(5430)
–Automatisch
–Manuell

•Volume
– Annuler
– Enregistrer
–Réglage usine

• Contraste
– Annuler
– Enregistrer
–Réglage usine

•Langue
–English
–Deutsch
–Français
–Español

• Mise en veille
–Marche
–Arrêt
–Réglage du temps

• Ouverture

couvercle (5430)
–Automatique
–Manuelle

•Volumen
–Cancelar
–Guardar
– Est. de Fábrica

• Contraste
–Cancelar
–Guardar
– Est. de Fábrica

•Idioma
–English
–Deutsch
–Français
–Español

• Standby
– Encendido
–Apagado
–Establecer tiempo

• Apertura de la tapa

(5430)
–Automática
–Manual

5430: only for Centrifuge 5430
5430 R: only for Centrifuge 5430 R

Operating controls and function

Centrifuge 5430/5430 R

English (EN)

6.2 Settings in the device menu

6.2.1 Programs

The Centrifuge 5430/5430 R has more than 50 programmable memory locations.

Load program Load the selected program. This appears in the display with number and name

and can be started immediately using the <>start/stop key.
When selecting a program with a too high g-force (rcf)/speed for the rotor
used, it flashes and a safety message appears.

Save program Save the set centrifugation parameters (centrifugation duration, temperature

(only 5430 R), g-force (rcf)/speed, soft ramp and At set rpm) under the selected
number. In addition, you can assign a program name with a maximum of 20
characters. The centrifuging duration, the temperature (only 5430 R) and the
g-force/rotational speed can still be changed in this menu using the
corresponding arrow buttons time, temp (only 5430 R) and speed.

Delete program Delete the selected program.

Program numbers 1 to 5 cannot be deleted.

47

These functions are only available with the centrifuge at a standstill.

If the program memory is empty the menu item Delete program is exited automatically after the last
program has been deleted. You will furthermore be unable to call up this menu item if the program memory
is empty.

Program numbers which are already occupied can be overwritten.

6.2.2 Use program keys

You can also save and load Programs 1 to 5 directly by pressing the program keys:

6.2.2.1 Load program

 Press the desired program key briefly.

The pressed program key illuminates in blue, the parameters are displayed.

By pressing again, you can exit the selected program again. The blue light of the key will then go out. The
parameters of the most recent centrifugation are then displayed again.

6.2.2.2 Save program

1. Set the centrifugation parameters (centrifugation duration, temperature (only 5430 R), g-force (rcf)/
speed, soft ramp and At set rpm).

2. Press the desired program key for at least 2 seconds.

A signal tone sounds and the program key you pressed lights up.

The centrifuging parameters are saved under the appropriate program number (1-5).

48

Operating controls and function

Centrifuge 5430/5430 R
English (EN)

6.2.3 Other menu items

Menu item/meaning Setting Function Display

Soft ramp

Reduce speed of acceleration and braking
ramp.
Not used for Short Spin centrifuging.

Key lock

Set the current centrifugation parameters
permanently to prevent the time,
temperature (only 5430 R), g-force (rcf) or
speed, soft ramp and At set rpm from
being unintentionally modified.

At set rpm

Set start of centrifuging run time.

Short Spin

Before the start of a short run (see
Short-spin centrifugation on p. 40) it is
possible to switch between the maximum
and currently set g-force (rcf) or speed.
Soft ramp is not used for Short Spin
centrifuging.

Continuous cooling (only 5430 R)
Time limitation of continuous cooling (see
p. 38) .
Continuous cooling is only activated when
the rotor is stopped and the centrifuge lid
is closed.

Fast Temp pro (only 5430 R)
Time and temperature programming for
the automatic temperature control run.
The selection Once/Repeatedly only
appears if no Fast Temp pro has been set
(see FastTemp pro on p. 37).

on Rotor accelerates and brakes slowly.

off Rotor accelerates and brakes rapidly.

on Set the centrifugation parameters

permanently.

off Release the permanent settings.

on The set time is counted down only

once 95% of the specified g-force (rcf)
or speed has been reached.

off The set time is counted down

immediately.

Maximum
value

Current
value

8 h Preset value.

∞ Endless operation of continuous

Once Set the date and time for the start of a

Repeatedly Set the week days and the common

Short-spin run at maximum g-force
(rcf) or speed of the rotor used.

Short run at set g-force (rcf)/speed.

cooling.
Caution! Icing possible! Set at own
risk!

temperature control run.

start time for several repeated
temperature control runs.
Fast Temp pro is active from the date
set.

Operating controls and function

Centrifuge 5430/5430 R

English (EN)

6.2.4 Settings

Menu item/meaning Setting Function Display

Display

Select standard display or extended
display of centrifuging parameters.

Loudspeaker

Switch loudspeaker on and off.
In the event of error messages, a signal
tone sounds even if the loudspeaker is
switched off.

Volume

Adjust the speaker volume using the menu

arrow keys and in 5 stages.
The signal tone for error messages is
always issued at least at medium volume.

Date/time

Set date and time.
The system does not switch automatically
between summer and winter time.

Contrast

Adjust the display contrast using the menu

arrow keys and .

Language Set menu language (English, Deutsch,

Standby

Switch standby mode on and off.
If the centrifuge is not used during the set
time period and no continuous cooling
takes place (only 5430 R), it switches to
the standby mode (see p. 41).

Lid release (only 5430) Automatic Lid opens automatically at the end of

Standard
display

Extended
display

On Switch on loudspeaker.

Off Switch off loudspeaker.

Cancel Exit menu item without saving.

Save Save volume just set.

Default Restore default volume.

Cancel Exit menu item without saving.

Save Save the contrast just set.

Default Restore default contrast.

On Switch on standby mode.

Off Switch off standby mode.

Set time Using the arrow keys, set the time

Manual Lid remains closed at the end of

If the centrifuge is at rest the target
values are displayed, and during
centrifugation the actual values of run
time, temperature (only 5430 R) and
the g-force (rcf)/speed.

In addition to the standard display,
specified values are always shown at
the bottom of the display.

In the date display, set year (YYYY),
month (MM) and day (DD).
In the time display, set hours (hh) and
minutes (mm). Before setting the clock
time the time format is selected (12 h /
24 h).

Français or Español) . (see Set menu
language on p. 30).

after which the centrifuge should
automatically change to the standby
mode (1 to 60 min).

centrifuging when the rotor stops.

centrifuging when the rotor stops and
can be opened using the now flashing
key open.

49

50

Maintenance

Centrifuge 5430/5430 R
English (EN)

7 Maintenance

7.1 Prepare cleaning/disinfection

 Clean all accessible surfaces of the device and the accessories at least weekly and when contaminated.

 Clean the rotor regularly. This way the rotor is protected and the durability is prolonged.

 Furthermore, observe the notes on decontamination (see Decontamination before shipment on p. 53)

when the device is sent to the authorized Technical Service for repairs.

The procedure described in the following chapter applies to the cleaning as well as to the disinfection or
decontamination. The table below describes the steps required on top of this:

Cleaning Disinfecting/decontamination

1. Use a mild cleaning fluid to clean the accessible
surfaces of the device and the accessories.

2. Carry out the cleaning as described in the
following chapter.

1. Choose the disinfection method which
corresponds to the legal regulations and
guidelines in place for your range of
application. For example, use alcohol (ethanol,
isopropanol) or alcohol-based disinfectants.

2. Carry out the disinfection or decontamination as
described in the following chapter.

3. Then clean the device and the accessories.

If you have any further questions regarding the cleaning and disinfection or decontamination
or regarding the cleaning fluid to be used, contact the Eppendorf AG Application Support. The
contact details are provided on the back of this manual.

7.2 Cleaning/disinfection

DANGER! Electric shock as a result of penetration of liquid.

 Switch off the device and disconnect the power plug before starting cleaning or

disinfection work.

 Do not allow any liquids to penetrate the inside of the housing.
 Do not spray clean/spray disinfect the housing.
 Only plug the device back in if it is completely dry, both inside and outside.

NOTICE! Damage from the use of aggressive chemicals.

 Do not use any aggressive chemicals on the device or its accessories, such as strong and

weak bases, strong acids, acetone, formaldehyde, halogenated hydrocarbons or phenol.

 If the device has been contaminated by aggressive chemicals, clean it immediately using a

mild cleaning agent.

NOTICE! Corrosion due to aggressive cleaning agents and disinfectants.

 Do not use corrosive cleaning agents, aggressive solvents or abrasive polishes.
 Do not incubate the accessories in aggressive cleaning agents or disinfectants for longer

periods.

Maintenance

Centrifuge 5430/5430 R

English (EN)

NOTICE! Damage from UV and other high-energy radiation.

 Do not use UV, beta, gamma, or any other high-energy radiation for disinfecting.
 Avoid storage in areas with strong UV radiation

Autoclaving

All rotors, rotor lids and adapters, except the A-2-MTP rotor, can be autoclaved (121 °C,
20 min).
Replace the lids of the aerosol-tight rotors after a maximum of 50 autoclaving cycles.
Only QuickLock rotor lid: Only the seal needs to be replaced after a maximum of 50
autoclaving cycle.

Swing-bucket rotor

• Before cleaning the rotor, remove old pivot grease from grooves and pivots.

• Make sure that the grooves and pivots are clean. Dirty grooves and pivots prevent carriers
from swinging out evenly.

• After cleaning, lubricate the pivots of the rotor and the grooves of the buckets with pivot
grease (order no. Int.: 5810 350.050/North America: 022634330) so that the carriers can
move freely in a swinging manner.

51

7.2.1 Cleaning and disinfecting the device

1. Open the lid. Switch off the device with the mains/power switch. Disconnect the power plug from the
power supply.

2. Loosen the rotor nut by turning the rotor key counterclockwise.

3. Remove the rotor.

4. Clean and disinfect all accessible surfaces of the device, including the power cable, using a damp cloth
and the recommended cleaning agents.

5. Thoroughly clean the rubber seals of the rotor chamber with water.

6. Rub the dry rubber seal with glycerine or talcum powder to prevent it from becoming brittle. Other
components of the device, such as the lid latch, lid springs, motor shaft and rotor cone, must not be
lubricated.

7. Clean the motor shaft with a soft, dry and lint-free cloth. Do not lubricate the motor shaft.

8. Check the motor shaft for damage.

9. Inspect the device for corrosion and damage.

10. Leave the centrifuge lid open when the device is not being used.

11. Only connect the device to the power supply if it is fully dry inside and out.

7.2.2 Cleaning and disinfecting the rotor

1. Inspect the rotor and accessories for damage and corrosion. Do not use any damaged rotors or
accessories.

2. Clean and disinfect the rotors and accessories with the recommended cleaning agents.

3. Use a bottle brush to clean and disinfect the rotor bores.

52

Maintenance

Centrifuge 5430/5430 R
English (EN)

4. Clean and disinfect the rotor lids. 
ONLY QuickLock: It is imperative that the sealing ring be removed during this process so that the
groove below it can be thoroughly cleaned.

5. Rinse the rotors and accessories thoroughly with distilled water. Rinse the rotor bores of fixed-angle
rotors particularly thoroughly.

Do not immerse the rotor in liquid as liquid can get trapped inside the cavities.

6. Place rotors and accessories on a cloth to dry. Place fixed-angle rotors with the rotor bores facing
downwards to allow the bores to also dry.

7. Correctly reinsert the rotor lid sealing ring in the clean and dry groove.

8. Clean the rotor cone with a soft, dry and lint-free cloth. Do not lubricate the rotor cone.

9. Inspect the rotor cone for damage.

10. Place the dry rotor onto the motor shaft.

11. Tighten the rotor nut firmly by turning it clockwise with the rotor key.

12. Load the fixed-angle rotor with the cleaned adapters or the swing-bucket rotor with the cleaned
buckets and adapters, if necessary.

13. Leave the rotor lid open when the rotor is not being used.

7.3 Additional service instructions for Centrifuge 5430 R

 Empty and clean the condensation water tray regularly and especially after liquid spillage in the rotor

chamber. Pull out the tray for condensation water from the left side of the centrifuge.

 Clean the condensation water drain on a regular basis, e.g., using a bottle brush.

 Regularly free the rotor chamber ice formations via thawing, by leaving the centrifuge lid open or

carrying out a short temperature control run at approx. 30 °C.

 Leave the centrifuge lid open when not in use for a long period.

 Wipe up condensate in the rotor chamber using a soft, absorbent cloth.

 Remove dust deposits from the ventilation slits of the centrifuge using a brush or swab at the latest

every six months. First switch off the device and remove the power plug.

 Regularly check the gas spring of the centrifuge lid for proper functioning.

A defective gas spring is an insufficient support for the centrifuge lid and could cause injury if the
centrifuge lid falls down. We recommend that the gas spring be replaced by a service technician every
2 years.

Maintenance

Centrifuge 5430/5430 R

English (EN)

7.4 Glass breakage

When using glass tubes there is a risk of glass breakage in the rotor chamber. The resulting glass splinters
are swirled around in the rotor chamber during centrifugation and have a sandblasting effect on the rotor
and accessories. The smallest glass particles become lodged in the rubber parts (e.g., the motor guide, the
rotor chamber gasket, and the rubber mats of adapters).

NOTICE! Glass breakage in the rotor chamber

Glass tubes in the rotor chamber may break if the g-force is too high. Broken glass can
damage the rotor, accessories and samples.

 Please note the manufacturer's information on the recommended centrifugation

parameters (load and speed).

Effects of glass breakage in the rotor chamber:

• Fine black metal abrasion in the rotor chamber (in metal rotor chambers)

• The surfaces of the rotor chamber and accessories are scratched.

• The chemical resistance of the rotor chamber is reduced.

• Contamination of samples

• Wear on rubber parts

53

How to proceed in case of glass breakage

1. Remove all splinters and glass powder from the rotor chamber and accessories.

2. Thoroughly clean the rotor and rotor chamber. Thoroughly clean the bores of the fixed-angle rotors, in
particular.

3. If required, replace rubber mats and adapters to prevent any further damage.

4. Regularly check the rotor bores for deposits and damage.

7.5 Fuses

The fuse holder is located under the mains power socket.

1. Pull out the power plug.

2. Remove the fuse holder towards the rear.
The two fuses can now be reached. The fuses can be replaced.

Instead of removable fuses, the Centrifuge 5430 R features a thermal overcurrent protective switch. The
mains power switch jumps to the '0' switch setting if the overcurrent protective switch is triggered.

1. Use the mains power switch to switch the device back on after more than 20 s.

2. If the mains power switch returns to the '0' switch setting, contact Technical Support.

7.6 Decontamination before shipment

If you are shipping the device to the authorized Technical Service for repairs or to your authorized dealer
for disposal please note the following:

WARNING! Risk to health from contaminated device

1. Follow the instructions in the decontamination certificate. It is available as a PDF file on
our webpage (www.eppendorf.com/decontamination

2. Decontaminate all the parts you would like to dispatch.

3. Include the fully completed decontamination certificate in the package.

).

54

Trou bleshooting

Centrifuge 5430/5430 R
English (EN)

8 Troubleshooting

If you cannot remedy an error with the recommended measures, please contact your local Eppendorf
partner. The contact address can be found online at: www.eppendorf.com

.

8.1 General errors

Symptom/message Cause Remedy

No display. No power connection.

No display. Power failure.

Lid of the device
cannot be opened.

Lid of the device
cannot be opened.

Device cannot be
started.

Device shakes
when it starts up.

Centrifuge brakes
during a short run
centrifugation,
although the short
key is pressed.

Tempera ture
display flashes.
(only for 5430 R)

Rotor is still running.

Power failure. 1. Check the mains fuse of the device

Lid of the device is not closed.

Rotor loaded unsymmetrically. 1. Stop the device and load

The short key was released briefly more
than twice (protective function for the
drive).

Temperature deviation from the set
value: ±3 °C.

 Check mains/power connection.

 Check the mains fuse of the device

(see Fuses on p. 53).

 Check the mains fuse of the

laboratory.

 Wait for rotor to stop.

(see Fuses on p. 53).

2. Check the mains fuse of the
laboratory.

3. Activate the emergency lid release
(see p. 57).

 Close device lid.

symmetrically.

2. Restart device.

 Press the short key continuously

during a short run centrifugation.

 Check the settings.
 Check unhindered air circulation

through the air slots.

 Thaw ice or switch off device and

allow it to cool down.

Troubleshooting

Centrifuge 5430/5430 R

English (EN)

8.2 Error messages

If one of the following error messages appears, proceed as follows:

1. Remove fault (see Remedies).

2. Press open key to clear the error message.

3. If necessary, repeat centrifugation.

Some errors can have various causes. The actual cause is described in the message in the device display.

Symptom/message Cause Remedy

Note A
Lid latch

Note B
Imbalance

Note C
Rotor detection

Centrifuge lid could not be locked.

Rotor loaded unsymmetrically.

Set g-force/speed too high, e.g. after a
rotor change (see Automatic rotor
detection on p. 32).

 Try again to close centrifuge lid.

 Load the rotor symmetrically and

balance it.

1. Check the g-force/speed.

2. Repeat the run.

55

Symptom/message Cause Remedy

Error 1
Rotor detection

Error 2
Electronics fault

Error 3
Speed control

Error 3
Speed control

Error 5
Lid latch

Error 6
Drive fault

Error 6
Drive fault

Error 7
Speed control

Error 8
Speed control

Error 9 to Error 14 Electronics fault.

Rotor not detected.

 Check rotor.
 If this error message appears again,

test with a different rotor.

Electronics fault.

Error in speed measuring system.

Error in speed measuring system.

Prohibited opening of lid or lid switch is
defective during a run.

Drive fault.

 Switch centrifuge off and back on

again after > 20 s.

 Insert and tighten rotor.

 Wait for displayed time.

1. Wait for rotor to stop.

2. Open and close again the lid of the
device.

3. Repeat the run.

 Repeat the run.
 If this error message appears again,

switch centrifuge off and back on
again after > 20 s.

Drive overheated.

Major deviation in the speed control. 1. Wait for rotor to stop.

•Drive fault.

• Rotor loose.

•Incorrect rotor.

 Allow the drive to cool down for at

least 15 min.

2. Tighten rotor.

1. Wait for rotor to stop.

2. Tighten rotor.

3. Repeat the run.

 Switch centrifuge off and back on

again after > 20 s.

56

Trou bleshooting

Centrifuge 5430/5430 R
English (EN)

Symptom/message Cause Remedy

Error 16 to Error 17
Electronics fault

Error 18
Room Temp. of
rotor chamber

(only 5430 R)

Error 18
Room Temp. of
rotor chamber

(only 5430 R)

Error 22
Electronics fault

(only 5430 R)

Error 25
Mains/power failure

Error 26
Electronics fault

(only 5430 R)

Error 27
Electronics fault

(only 5430 R)

Error 28
Electronics fault

Error 30
Lid latch

Error 30
Lid latch

Error 30
Lid latch

Electronics fault.  Switch centrifuge off and back on

again after > 20 s.

Temperature deviation from set value in
the rotor chamber: ΔT > 16 °C.

Temperature deviation from set value in
the rotor chamber: ΔT > 50 °C.

Electronics fault.

Mains/power failure during a run.

Electronics fault.

Electronics fault.

Electronics fault.

Centrifuge lid could not be locked.

Centrifuge lid could not be released.

Centrifuge lid has not been opened wide
enough.

 Allow the device to cool down and

repeat cycle.

 Allow the device to cool down and

repeat cycle.

 Switch centrifuge off and back on

again after > 20 s.

 Check the power supply.

 Switch centrifuge off and back on

again after > 20 s.

 Switch centrifuge off and back on

again after > 20 s.

 Press the open key.

 Try again to close centrifuge lid.

 Switch the device off and back on

again.

If the error occurs again:

1. Switch off the device.

2. Activate the emergency lid release
(see Emergency release on p. 57).

 Open the centrifuge lid wider by

hand.

Troubleshooting

Centrifuge 5430/5430 R

English (EN)

8.3 Emergency release

If the centrifuge lid cannot be opened, you can activate the emergency release manually.

WARNING! Risk of injury from rotating rotor.

If the emergency release of the lid is operated, the rotor may continue rotating for several
minutes.

 Wait until the rotor stop before operating the emergency release.
 Check the monitoring glass in the centrifuge lid.

Use the rotor key delivered with the Centrifuge 5430 for the emergency release.
The rotor key for the rotor FA-45-24-11-HS is not suitable for this purpose.

1. Pull the mains/power plug.

2. Carry out the following steps for the emergency release on both the left side and right side of the
centrifuge (see Fig. 1 and Fig. 2).

3. Only 5430: Remove the plastic cover for the emergency release.

4. Insert the centrifuge rotor key in the rear hexagonal opening until a noticeable resistance is felt.

5. Slightly press and turn the rotor key counterclockwise five to ten revolutions, as depicted on the
openings of the emergency release.

This will release the centrifuge lid.

6. Open the centrifuge lid.

7. Remove the rotor key and put the plastic covers back on (Centrifuge 5430).

57

58

Transport, storage and disposal

Centrifuge 5430/5430 R
English (EN)

9 Transport, storage and disposal

9.1 Transport

CAUTION! Risk of injury due to lifting and carrying heavy loads

The device is heavy. Lifting and carrying the device can lead to back injuries.

 Transport and lift the device with an adequate number of helpers only.
 Use a transport aid to transport the device.

 Remove the rotor from the centrifuge before transport.

 Use the original packaging for transport.

Air temperature Relative humidity Atmospheric pressure

General transport -25 °C – 60 °C 10 % – 75 % 30 kPa – 106 kPa

Air freight -20 °C – 55 °C 10 % – 75 % 30 kPa – 106 kPa

9.2 Storage

Air temperature Relative humidity Atmospheric pressure

In transport packaging -25 °C – 55 °C 10 % – 75 % 70 kPa – 106 kPa

Without transport
packaging

-5 °C – 45 °C 10 % – 75 % 70 kPa – 106 kPa

9.3 Disposal

In case the product is to be disposed of, the relevant legal regulations are to be observed.

Information on the disposal of electrical and electronic devices in the European Community:

Within the European Community, the disposal of electrical devices is regulated by national regulations
based on EU Directive 2002/96/EC pertaining to waste electrical and electronic equipment (WEEE).

According to these regulations, any devices supplied after August 13, 2005, in the business-to-business
sphere, to which this product is assigned, may no longer be disposed of in municipal or domestic waste. To
document this, they have been marked with the following identification:

Because disposal regulations may differ from one country to another within the EU, please contact your
supplier if necessary.

10 Technical data

10.1 Power supply

Centrifuge 5430

Mains connection 230 V, 50 to 60 Hz

120 V, 50 to 60 Hz
100 V, 50 to 60 Hz

Current consumption 3 A (230 V)

6 A (120 V)
7 A (100 V)

Power consumption Maximum 475 W

EMC: interference emission (radio
interference)

EMC: noise immunity EN 61326

Overvoltage category II

Protection class 1

Fuses 4.0 AT (230 V)

EN 61326 - category B

8.0 AT (120 V/100 V)

Technical data

Centrifuge 5430/5430 R

English (EN)

59

Centrifuge 5430 R

Mains connection 230 V, 50 to 60 Hz

120 V, 50 to 60 Hz
100 V, 50 to 60 Hz

Current consumption 6 A (230 V)

12 A (120 V/ 100 V)

Power consumption Maximum 1050 W

EMC: Interference emission (radio
interference)

EMC: Noise immunity EN 61326

Overvoltage category II

Protection class 1

Fuses Thermal overcurrent protective switch 7 A (230 V)

EN 61326 – Class B (230 V/120 V)
EN 61326 – Class A (100 V)

Thermal overcurrent protective switch 15 A (120 V/100 V)

10.2 Ambient conditions

Ambience For indoor use only.

Ambient temperature Centrifuge 5430: 4 to 40°C

Centrifuge 5430 R: 10 to 40°C

Maximum relative humidity 75 %, non-condensing humidity

Atmospheric pressure Use up to an altitude of 2000 m above MSL.

Degree of pollution 2

60

Technical data

Centrifuge 5430/5430 R
English (EN)

10.3 Weight/dimensions

Centrifuge 5430

Dimensions Width: 335 mm (11.2 in.)

Depth: 415 mm (16.3 in.)
Height: 250 mm (9.84 in.)

Weight without rotor 29 kg (63.9 lbs.)

Noise level < 60 dB(A) *

Centrifuge 5430 R

Dimensions Width: 380 mm (15.0 in.)

Depth: 640 mm (25.2 in.)
Height: 296 mm (11.7 in.)

Weight without rotor 56 kg (123.5 lbs.)

Noise level < 60 dB(A) *

*) The noise level was measured frontally in a sound measuring room with accuracy class 1, 1 m from the
device and at lab bench height.

10.4 Application parameters

Run time 30 s to 99:59 h, infinity ( )

Adjustable to 10 min in 0.5 min increments, then increments of
1 min

Temperature of 5430 R -11°C to 40°C

Relative centrifugal force (or rcf) 1 to 30.130 × g

Adjustable in increments of 10 × g up to 3,000 × g, then in
increments of 100 × g

Speed 100 to 17,500 rpm

Adjustable in increments of 10 rpm up to 5,000 rpm, then in
increments of 100 rpm

Maximum load 48 tubes with 2.0 mL each or 6 conical tubes with 50 mL each

Maximum kinetic energy 10,000 nm

Compulsory test log book No

Permitted density of the material for
centrifuging at max. g-force/rpm and
max. load

1.2 g/mL

Technical data

Centrifuge 5430/5430 R

English (EN)

61

Rotor Lowest achievable temperature

-11°C set
23°C ambient temperature
60 min run time

FA-45-48-11 < 0 °C 12 700 rpm

F-45-48-11 < 0 °C 12 700 rpm

FA-45-30-11 < 0 °C 14 000 rpm

F-45-30-11 < 0 °C 14 000 rpm

FA-45-24-11-HS < 5 °C ? rpm

FA-45-24-11-Kit < 0 °C 13 200 rpm

F-45-64-5-PCR < 0 °C 11 800 rpm

F-45-18-17-Cryo < 0 °C 8 900 rpm

FA-45-16-17 < 0 °C 14 200 rpm

F-35-6-30 < 0 °C 7 8300 rpm

A-2-MTP < 0 °C 4 680 rpm

S-24-11-AT < 0 °C 12 700 rpm

Acceleration times and braking times of Centrifuge 5430/5430 R

Rotor Acceleration time/Deceleration time Without soft ramp

Acceleration time/Deceleration time With soft ramp

230 V 120 V 100 V

FA-45-48-11
F-45-48-11

FA-45-30-11
F-45-30-11

FA-45-24-11-Kit ≤ 15 / 16 s

FA-45-16-17 ≤ 20 / 20 s

S-24-11-AT ≤ 13 / 16 s

FA-45-24-11-HS ≤ 21 / 16 s

F-45-64-5-PCR ≤ 12 / 15 s

F-45-18-17-Cryo ≤ 8 / 11 s

F-35-6-30 ≤ 23 / 23 s

A-2-MTP ≤ 18 / 21 s

≤ 20 / 20 s
≤ 61 / 65 s (SOFT)

≤ 15 / 15 s
≤ 61 / 65 s (SOFT)

≤ 78 / 90 s (SOFT)

≤ 61 / 66 s (SOFT)

≤ 61 / 66 s (SOFT)

≤ 60 / 65 s (SOFT)

≤ 62 / 65 s (SOFT)

≤ 77 / 85 s (SOFT)

≤ 62 / 67 s (SOFT)

≤ 63 / 67 s (SOFT)

≤ 20 / 20 s
≤ 61 / 65 s (SOFT)

≤ 15 / 15 s
≤ 61 / 65 s (SOFT)

≤ 15 / 16 s
≤ 78 / 90 s (SOFT)

≤ 20 / 20 s
≤ 61 / 66 s (SOFT)

≤ 13 / 16 s
≤ 61 / 66 s (SOFT)

≤ 21 / 16 s
≤ 60 / 65 s (SOFT)

≤ 12 / 15 s
≤ 62 / 65 s (SOFT)

≤ 8 / 11 s
≤ 77 / 85 s (SOFT)

≤ 23 / 23 s
≤ 62 / 67 s (SOFT)

≤ 18 / 21 s
≤ 63 / 67 s (SOFT)

Speed to safely maintain 4°C sample
temperature

4°C set
23 °C ambient temperature

≤ 27 / 20 s
≤ 61 / 65 s (SOFT)

≤ 20 / 15 s
≤ 61 / 65 s (SOFT)

≤ 20 / 16 s
≤ 78 / 90 s (SOFT)

≤ 30 / 20 s
≤ 61 / 66 s (SOFT)

≤ 16 / 16 s
≤ 61 / 66 s (SOFT)

≤ 30 / 16 s
≤ 60 / 65 s (SOFT)

≤ 15 / 15 s
≤ 62 / 65 s (SOFT)

≤ 8 / 11 s
≤ 77 / 85 s (SOFT)

≤ 27 / 27 s
≤ 62 / 67 s (SOFT)

≤ 18 / 21 s
≤ 63 / 67 s (SOFT)

62

Ordering Information

Centrifuge 5430/5430 R
English (EN)

11 Ordering Information

11.1 Centrifuge 5430

Order no.
(International)

5427 000.011 022620533 230 V / 50 – 60 Hz
– 022620584 120 V / 50 – 60 Hz, with US-plug

5427 000.216 022620525 230 V / 50 – 60 Hz
– 022620509 120 V / 50 – 60 Hz, with US-plug

– 022620541 120 V / 50 – 60 Hz, with US-plug

– 022620568 120 V / 50 – 60 Hz, with US-plug

5427 000.615 022620540 230 V / 50 – 60 Hz
– 022620596 120 V / 50 – 60 Hz, with US-plug

5427 000.410 - 230 V / 50 – 60 Hz
– 022620511 120 V / 50 – 60 Hz, with US-plug

– 022620557 120 V / 50 – 60 Hz, with US-plug

– 022620572 120 V / 50 – 60 Hz, with US-plug

Order no.
(North America)

Description

Centrifuge 5430, Keypad

without rotor

Centrifuge 5430, Keypad

with rotor FA-45-30-11 incl. rotor lid

Centrifuge 5430, Keypad

with rotor FA-45-24-11-Kit incl. rotor lid

Centrifuge 5430, Keypad

with rotor A-2-MTP incl. wind shield upper shell

Centrifuge 5430, Knobs

without rotor

Centrifuge 5430, Knobs

with rotor FA-45-30-11 incl. rotor lid

Centrifuge 5430, Knobs

with rotor FA-45-24-11-Kit incl. rotor lid

Centrifuge 5430, Knobs

with rotor A-2-MTP incl. wind shield upper shell

11.2 Centrifuge 5430 R

Order no.
(International)

5428 000.210 022620678 230 V / 50 – 60 Hz
– 022620667 120 V / 50 – 60 Hz, with US-plug

5428 000.015 022620612 230 V / 50 – 60 Hz
– 022620601 120 V / 50 – 60 Hz, with US-plug

Order no.
(North America)

Description

Centrifuge 5430 R, Keypad

without rotor

Centrifuge 5430 R, Keypad

with rotor FA-45-30-11 incl. rotor lid

Ordering Information

Centrifuge 5430/5430 R

English (EN)

63

Order no.
(International)

– 022620645 120 V / 50 – 60 Hz, with US-plug

5428 000.619 022620690 230 V / 50 – 60 Hz
– 022620689 120 V / 50 – 60 Hz, with US-plug

5428 000.414 022620634 230 V / 50 – 60 Hz
– 022620623 120 V / 50 – 60 Hz, with US-plug

– 022620656 120 V / 50 – 60 Hz, with US-plug

Order no.
(North America)

Description

Centrifuge 5430 R, Keypad

with rotor A-2-MTP incl. wind shield upper shell

Centrifuge 5430 R, Knobs

without rotor

Centrifuge 5430 R, Knobs

with rotor FA-45-30-11 incl. rotor lid

Centrifuge 5430 R, Knobs

with rotor A-2-MTP incl. wind shield upper shell

11.3 Rotors, rotor lids and seals

11.3.1 Rotors with QuickLock rotor lid

Rotor FA-45-48-11

Order no.
(International)

5427 754.008 5427754008 aerosol-tight, angle 45°, 48 places, max. tube diameter 11 mm,

5427 762.000 5427762000 aerosol-tight, aluminum

5409 718.002 5409718002 set of 5 pieces

Order no.
(North America)

Description

Fixed-angle rotor FA-45-48-11

incl. rotor lid (aluminum)

Rotor lid for FA-45-48-11

Seal for rotor lid

FA-45-48-11 (5427 R/5430/5430 R)

Rotor FA-45-30-11

Order no.
(International)

5427 753.001 5427753001 aerosol-tight, angle 45°, 30 places, max. tube diameter 11 mm,

5427 761.004 5427761004 aerosol-tight, aluminum

5820 762.004 5820762004 Set of 5 pieces

Order no. (North
America)

Description

Fixed-angle FA-45-30-11

incl. rotor lid (aluminum)

Rotor lid for FA-45-30-11

Seal for rotor lid

FA-45-30-11 (5427 R/5430/5430 R)

64

Ordering Information

Centrifuge 5430/5430 R
English (EN)

Rotor FA-45-24-11 Kit

Order no.
(International)

5427 752.005 5427752005 aerosol-tight, angle 45°, 24 places, max. tube diameter 11 mm,

5427 760.008 5427760008 aerosol-tight, aluminum

5820 767.006 5820767006 Set of 5 pieces

Rotor FA-45-16-17

Order no.
(International)

5427 750.002 5427750002 aerosol-tight, angle 45°, 16 places, max. tube diameter 17 mm,

5427 751.009 5427751009 aerosol-tight, aluminum

5409 717.006 5409717006 Set of 5 pieces

Order no.
(North America)

Order no.
(North America)

Description

Fixed-angle rotor FA-45-24-11-Kit

incl. rotor lid (aluminum)

Rotor lid for FA-45-24-11-Kit

Seal for rotor lid

FA-45-24-11-Kit (5427 R/5430/5430 R), FA-45-48-11,
FA-45-20-17 (5804/5804 R/5810/5810 R)

Description

Fixed-angle rotor FA-45-16-17

incl. rotor lid (aluminum)

Rotor lid for FA-45-16-17

Seal for rotor lid

FA-45-24-11 (5427 R), FA-45-16-17 (5430/5430 R)

Rotor S-24-11-AT

Order no.
(International)

5427 757.007 5427757007 aerosol-tight, steel, angle 90°, 24 places, max. tube diameter

5427 758.003 5427758003 aerosol-tight, aluminum

5409 719.009 5409719009 set of 5 pieces

5409 721.003 5409721003 set of 2 pieces

Order no.
(North America)

Description

Swing-bucket rotor S-24-11-AT

11 mm, incl. rotor lid (aluminum)

Rotor lid for S-24-11-AT

Seal for rotor lid

S-24-11-AT (5427 R/5430/5430 R)

Tube holder for S-24-11-AT

for 4 × 1,5 mL/2,0 mL Eppendorf tubes

11.3.2 Rotors with rotor lid thread

Rotor F-45-48-11

Ordering Information

Centrifuge 5430/5430 R

English (EN)

65

Order no.
(International)

5409 712.004 5409712004 aluminum, angle 45°, 48 places, max. tube diameter 11 mm,

5409 713.000 5409713000 Polypropylene

Rotor FA-45-30-11

Order no.
(International)

5427 719.008 022654063 aerosol-tight, PTFE-coated, aluminum

Rotor F-45-30-11

Order no.
(International)

5427 712.003 022654004 PTFE-coated, angle 45°, 30 places, max. tube diameter

5427 718.001 022654021 Polypropylene

Order no.
(North America)

Order no.
(North America)

Order no.
(North America)

Description

Rotor F-45-48-11

incl. rotor lid (polypropylene)

Rotor lid for F-45-48-11

Description

Rotor lid

for F-45-30-11

Description

Rotor F-45-30-11

11 mm, incl. rotor lid (polypropylene)

Rotor lid

for F-45-30-11

Rotor FA-45-24-11-HS

Order no.
(International)

5427 710.000 022654080 aerosol-tight, PTFE-coated, angle 45°, 24 places, max. tube

5427 711.007 022654101 aerosol-tight, PTFE-coated, aluminum

Order no.
(North America)

Description

Rotor FA-45-24-11-HS

diameter 11 mm, incl. rotor lid (aluminum), incl. rotor key

Rotor lid

for FA-45-24-11-HS

66

Ordering Information

Centrifuge 5430/5430 R
English (EN)

Rotor FA-45-24-11-Kit

Order no.
(International)

5427 704.000 022654144 aerosol-tight, aluminum

Rotor F-45-64-5-PCR

Order no.
(International)

5427 714.006 022654209 angle 45°, 64 places, max. tube diameter 5 mm, incl. rotor lid

5427 720.006 022654225 aluminum

Rotor F-45-18-17-Cryo

Order no.
(International)

5427 705.007 022654161 angle 45°, 18 places, max. tube diameter 17 mm, incl. rotor lid

5427 707.000 022654187 Polypropylene

Order no.
(North America)

Order no.
(North America)

Order no.
(North America)

Description

Rotor lid

for FA-45-24-11-Kit

Description

Rotor F-45-64-5-PCR

(aluminum) and adapters

Rotor lid

for F-45-64-5-PCR

Description

Rotor F-45-18-17-Cryo

(polyproplene) and adapters

Rotor lid

for F-45-18-17-Cryo

Rotor F-35-6-30

Order no.
(International)

5427 716.009 022654306 angle 35°, 6 places, max. tube diameter 30 mm, incl. rotor lid

5427 739.009 5427739009 angle 35°, 6 places, max. tube diameter 30 mm, incl. rotor lid

5427 715.002 022654322 aluminum

Order no.
(North America)

Description

Rotor F-35-6-30

(aluminum) and adapters for 15/50 mL conical tubes

Rotor lid

for F-35-6-30

11.3.3 Rotors with rotor lid for attaching

A-2-MTP rotor

Ordering Information

Centrifuge 5430/5430 R

English (EN)

67

Order no.
(International)

5427 700.005 022634403 with 2 buckets and windshield upper shell

5427 722.009 022634420 Set of 2

5427 725.008 022654446 aluminum

The Aerosol tightness is tested and certified by the Centre of Emergency Preparedness and
Response, Health Protection Agency, Porton Down (UK).

Order no.
(North America)

Description

Rotor A-2-MTP

Spare MTP buckets for A-2-MTP

Wind shield upper shell for A-2-MTP

11.4 Accessories

11.4.1 Adapter

Order no.
(International)

5425 715.005 022636260 for 1 PCR tube (0.2 mL, max. Ø 6 mm), set of 6
5425 717.008 022636243 for 1 sample tube (0.4 mL, max. Ø 6 mm), set of 6
5425 716.001 022636227 for 1 sample tube (0.5 mL, max. Ø 6 mm) or 1 Microtainer

5427 717.005 022654241 for PCR strips, set of 4 pieces

5702 752.002 022639498 for cryo tubes (max. Ø 13 mm) and sealable centrifuge tubes

5427 708.006 5427708006 for 1.5 mL HPLC vials, 18 pieces

5427 740.007 5427740007 13 × 65-89, set of 2
5427 741.003 5427741003 13 × 90-110, set of 2
5427 746.005 5427746005 for Eppendorf Tubes 5.0 mL, set of 2
5427 726.004 022654365 for 15 mL conical tubes, set of 2
5427 732.004 022654512 for 7 - 15 mL round-bottom tubes and blood collection tubes,

5427 735.003 022654538 for 9 - 15 mL round-bottom tubes and blood collection tubes,

5427 742.000 5427742000 13 × 65-89, set of 2

Order no.
(North America)

Description

Adapter

used in FA-45-48-11, F-45-48-11, FA-45-30-11, F-45-30-11,
FA-45-24-11-HS and FA-45-24-11-Kit

(0.6 mL, max. Ø 8 mm), set of 6

Adapter

used in F-45-64-5-PCR

Adapter

used in F-45-18-17-Cryo

(max. Ø 12.2 mm), max. length 50 mm, set of 6

Adapter

used in F-35-6-30, small tube bore

set of 2

set of 2

Adapter

used in F-35-6-30, large tube bore

68

Ordering Information

Centrifuge 5430/5430 R
English (EN)

Order no.
(International)

5427 743.006 5427743006 13 × 90-110, set of 2
5427 747.001 5427747001 for Eppendorf Tubes 5.0 mL, set of 2
5427 727.000 022654349 for 50 mL conical tubes, set of 2
5427 723.005 022654331 for Centriplus centrifugal filter units, set of 6
5427 734.007 022654524 for 7 - 15 mL round-bottom tubes and blood collection tubes,

5427 738.002 022654545 for 9 - 15 mL round-bottom tubes and blood collection tubes,

5427 736.000 022654556 for 20 - 30 mL round-bottom tubes, set of 2
5427 737.006 022654567 for 50 mL round-bottom tubes, set of 2

5825 711.009 022638947 for 96-well PCR plates, set of 2
5825 713.001 022638955 for 384-well PCR plates, set of 2
5825 706.005 022638963 CombiSlide Adapter, set of 2

Order no.
(North America)

Description

set of 2

set of 2

Adapter

used in A-2-MTP

11.4.2 Other accessories

Order no.
(International)

5416 301.001 022634305 Standard
5427 730.001 5427730001 for rotor FA-45-24-11-HS

5427 728.007 5427728007

5810 350.050 022634330 Tube 20 mL

5428 850.418 022680452

Order no.
(North America)

Description

Rotor key

Rotor removal tool for Rotor F-35-6-30

Pivot grease

Tray for condensation water

11.4.3 Fuses for Centrifuge 5430

Order no.
(International)

5301 850.249 022654403 4 A (230 V), 2 pieces
5427 850.341 022654381 8.0 AT UL (120 V/100 V), 2 pieces

Order no.
(North America)

Description

Fuse

Centrifuge 5430/5430 Rseeon p.Fig.Tab.p.

Operating manual

Declarations and Certificates

Declarations and Certificates

Centrifuge 5430/5430 R

69

Declarations and Certificates

Centrifuge 5430/5430 R

83

84

Declarations and Certificates

Centrifuge 5430/5430 R

Annex

Centrifuge 5430/5430 R

Annex

Menu structure of Centrifuge 5430/5430 R

English Deutsch Français Español Display

Programs

• Load program

•Save program

• Delete program

Soft ramp

•On

•Off

Key lock

•On

•Off

Programme

•Programm laden

•Programm
speichern

•Programm löschen

Softrampe

•An

•Aus

Tastensperre

•An

•Aus

Programmes

•Charger prog.

• Enregistrer prog.

• Supprimer prog.

Rampe douce

•Marche

•Arrêt

Verrouilla. de touches

•Marche

•Arrêt

Programas

•Cargar programa

•Guardar programa

• Borrar programa

Rampa suave

• Encendido

•Apagado

Bloqueo del teclado

• Encendido

•Apagado

85

At set rpm

•On

•Off

Short spin

• Maximum speed

•Current speed

Continuous cooling
(5430 R)

•8 h

•∞

Fast Temp pro
(5430 R)

•One-time-use

• Repeated use

Settings

•D

i

splay
–Standard display
– Extended display

•Alarm
–On
–Off

At set rpm

•An

•Aus

Short Spin

• Maximaler Wert

• Aktueller Wert

Dauerkühlung
(5430 R)

•8 h

•∞

Fast Temp pro
(5430 R)

•Einmal

•Mehrmals

Einstellungen

•Anzeige
–Standardanzeige
– Erweiterte

Anzeige

•Lautsprecher
–An
–Aus

At set rpm

•Marche

•Arrêt

Short Spin

• Vitesse max

• Vitesse actuelle

Refrigération
continue (5430 R)

•8 h

•∞

Fast Temp pro
(5430 R)

•Une fois

•Plusieurs fois

Réglages

•Affichage
–Affichage

standard

–Affichage large

•Signal sonore
–Marche
–Arrêt

At set rpm

• Encendido

•Apagado

Short Spin

• Velocidad máximo

• Velocidad actual

Refrigeración
continua (5430 R)

•8 h

•∞

Fast Temp pro
(5430 R)

•Una vez

• Varias veces

Ajustes

•Indicador
–Indicador

estándar

–Indicador

extendido

•Altavoz
– Encendido
–Apagado

•Volume
–Cancel
–Save
–Default

• Date/Time • Datum/Uhrzeit • Date/Heure • Fecha/Hora

• Lautstärke
–Abbrechen
–Speichern
–Lieferzustand

•Volume
– Annuler
– Enregistrer
–Réglage usine

•Volumen
–Cancelar
–Guardar
– Est. de Fábrica

86

Annex

Centrifuge 5430/5430 R

English Deutsch Français Español Display

• Contrast
–Cancel
–Save
–Default

• Language
–English
–Deutsch
–Français
–Español

•Standby
–On
–Off
–Set time

•Lid release 

(5430)
–Automatic
–Manual

•Kontrast
–Abbrechen
–Speichern
–Lieferzustand

•Sprache
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Rotor F-35-6-30: Adapters for round-bottom tubes and blood taking systems

Adapter

(1)

Bottom
shape

Tube dimensions:
Ø × length
(min to max)

(2)

Rotor
bore

r

max

Max.
g-force
(rcf)

Ordering No.
(International)

Ordering No.
(North
America)

2.6 – 7 mL round 13 × (65 to 89) mm small 9.4 cm 6,443 × g 5427 740.007 5427740007

2.6 – 7 mL round 13 × (65 to 89) mm large 8.9 cm 6,100 × g 5427 742.000 5427742000

2.6 – 7 mL round 13 × (90 to 110) mm small 10.9 cm 7,471 × g 5427 741.003 5427741003

2.6 – 7 mL round 13 × (90 to 110) mm large 10.4 cm 7,129 × g 5427 743.006 5427743006

5 mL conical only 5 mL tubes small 9.1 cm 6,237 × g 5427 746.005 5427746005

5 mL conical only 5 mL tubes large 10.4 cm 7,129 × g 5427 747.001 5427747001

7 – 15 mL round 16 × (74 to 103) mm small 10.2 cm 7,005 × g 5427 732.004 022654512

7 – 15 mL round 16 × (85 to 115) mm large 10.2 cm 7,005 × g 5427 734.007 022654524

9 – 15 mL round 16.8 × (84 to 125) mmsmall 11.3 cm 7,745 × g 5427 735.003 022654538

9 – 15 mL round 16.8 × (84 to 125) mmlarge 10.8 cm 7,403 × g 5427 738.002 022654545

20 – 30 mL round 26 × (97 to 110) mm large 10.4 cm 7,087 × g 5427 736.000 022654556

50 mL round 29 × (100 to 125)

large 11.1 cm 7,581 × g 5427 737.006 022654567

mm

1) One tube per adapter, 6 per rotor. For the diameters 13, 16, and 16.8 mm, 12 tubes of the same type can
be simultaneously centrifuged in 6 of each small and large adapters.

2) Min. tube length below cap rim to max. tube length incl. cap.

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