5404 900.023-02/0810
Centrifuge 5424/5424 R
Operating manual
Copyright © 2011 Eppendorf AG, Hamburg. No part of this publication may be reproduced without the prior permission of
the copyright owner.
Trademarks
eppendorf is a registered trademark of Eppendorf AG, Hamburg, Germany.
Microtainer is a registered trademark of Becton Dickinson, Franklin Lakes, NJ, USA.
Trademarks are not marked in all cases with ™ or
®
in this manual.
Your Online Product Registration
Register your Eppendorf instruments online and get rewarded!
Learn more: www.eppendorf.com/myeppendorf
Not available in all countries.
Operating manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Declarations and Certificates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 176
5404 900.023-04/122011
You will find a detailed description of these figures in your language in Chapters 1.1/2.1 and 4.1/5.1.
1
3
4
2
1
5
6
7
2
8
9
1
3
4
10
11
2
1
5
7
6
9
8
2
Abb. 1: Front and rear view of the Ce ntrifuge 5424
Fig. 1: Front and rear view of the Centrifuge 5424
Abb. 2: Front and rear view of the Ce ntrifuge 5424 R
Fig. 2: Front and rear view of the Centrifuge 5424 R
1 Centrifuge lid 2 Window
3 Display 4 Control panel
5 Emergency release 6 ID plate
7 Mains switch 8 Power connection
9 Fuse holder 10 USB port
(only 5424 R, only for technical support)
11 Condensation water tray (only 5424 R)
Abb. 3: Control panel of the Centr ifuge 5424 / 5424 R
Fig. 3: Control panel of the Centrifuge 5424 / 5424 R
1 Call and select the menu parameters 2 Switch the centrifuging speed displayed (rpm/rcf)
3 Display 4 Short Spin centrifugation
5 Release lid 6 Start and stop centrifugation
7 Set the speed of centrifugation
8 Adjust the temperature (only 5424 R)
Dependent on device version designed as a key or
dial.
9 Adjust the centrifuging duration
Dependent on device version designed as a key or
10 Start the temperature control run fast temp
(only 5424 R)
dial.
11 Select the menu item
Abb. 4: Display of the Centrifug e 5424 / 5424 R
Fig. 4: Display of the Centrifuge 5424 R. The display of the Centrifuge 5424 is similar.
1 Centrifuging duration 2 Status of the key lock (LOCK)
3 Status of the function ATSET 4 Temperature (only 5424 R)
5 Soft ramp 6 g-force (rcf) or speed (rpm)
7 Status of the centrifuge 8 Status of the centrifugation speed display
9 Status of the loudspeaker
Centrifuge 5424 / 5424 R — Operating manual
Table of contents
Operating manual
Table of contents
1 User instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.1 Using this manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.2 Warning signs and hazard icons. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.2.1 Hazard symbols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.2.2 Degrees of danger . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.3 Abbreviations used. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
2 Product description. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
2.1 Main illustration. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
2.2 Delivery package . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
2.2.1 Centrifuge 5424 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
2.2.2 Centrifuge 5424 R. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
2.3 Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
2.4 Rotors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
2.4.1 Rcf display and calculation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
3 Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
3.1 Intended use. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
3.2 User profile . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
3.3 Application limits. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
3.3.1 Declaration concerning the ATEX directive (94/9/EC). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
3.3.2 Maximum service life for accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
3.4 Information on product liability . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
3.5 Warnings for intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
3.5.1 Personal injury or damage to the equipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
3.5.2 Incorrect handling of the centrifuge. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
3.5.3 Incorrect handling of the rotors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
3.5.4 Extreme strain on the centrifuging tubes. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
3.5.5 Aerosol-tight centrifugation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
3.6 Safety instructions on the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
4 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
4.1 Selecting the location . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
4.2 Installing the instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
5 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
5.1 Overview of operating controls. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
5.2 Device menu. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
5.3 Menu navigation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
5.4 Preparing for centrifugation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
5.4.1 Switch on centrifuge . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
5.4.2 Inserting the rotor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
5.4.3 Load the rotor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
5.4.4 Close centrifuge lid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
5.5 Cooling (only 5424 R). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
5.5.1 Temperature adjustment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
5.5.2 Temperature display . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
5.5.3 Temperature monitoring . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
5.5.4 Fast Temp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
5.5.5 Continuous cooling . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
5.6 Centrifuging . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
5.6.1 Centrifuging with time preset. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
5.6.
2 Centrifuging in continuous operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
5.6.3 Short spin centrifugation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
5.6.4 Removing the rotor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
Operating manual — Centrifuge 5424 / 5424 Rseeon p.Fig.Tab.p.
. . . . . . . . 25
EN
Operating manual
5
EN
Operating manual
Centrifuge 5424 / 5424 R — Operating manual
Table of contents
6 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
6.1 Prepare cleaning / disinfection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
6.2 Perform cleaning / disinfection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
6.2.1 Clean / disinfect device. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
6.2.2 Clean / disinfect rotor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
6.3 Additional service instructions for Centrifuge 5424 R. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
6.4 Glass breakage. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
6.5 Fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
6.6 Decontamination before shipping . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
7 Troubleshooting. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
7.1 General errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
7.2 Error messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
7.3 Emergency release. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
8 Transport, storage and disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
8.1 Transport . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
8.2 Storage. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
8.3 Disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
9 Technical data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
9.1 Power supply . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
9.2 Ambient conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
9.3 Weight / dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
9.4 Application parameters. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
9.4.1 Centrifuge 5424 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
9.4.2 Centrifuge 5424 R. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
10 Ordering information. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
10.1 Centrifuge 5424 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
10.2 Centrifuge 5424 R . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
10.3 Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
10.3.1 Rotors and rotor lids . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
10.3.2 Adapters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
10.3.3 Other accessories. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
10.4 Fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
6
Centrifuge 5424 / 5424 R — Operating manual
1 User instructions
1 User instructions
1.1 Using this manual
1.2 Warning signs and hazard icons
1.2.1 Hazard symbols
Operating manual — Centrifuge 5424 / 5424 Rseeon p.Fig.Tab.p.
Please read this operating manual completely before using the device for the first time.
Please view this operating manual as part of the product and keep it somewhere easily
accessible.
When passing the device on to third parties, be sure to include this operating manual.
If this manual is lost, please request another one. The current version can be found on our
website www.eppendorf.com.
The Centrifuge 5424 / 5424 R is available in two versions: keypad or rotary knobs. This
operating manual generally describes how to operate the keypad version. However, it also
applies to the rotary knob version.
Biohazard Explosion
EN
Operating manual
Electric shock Crushing
Hazard point Material damage
1.2.2 Degrees of danger
The degree of danger is a part of a safety note and distinguishes the possible results of
non-observance from each other.
DANGER Will lead to severe injuries or death.
WARNING May lead to severe injuries or death.
CAUTION May lead to light to moderate injuries.
NOTICE May lead to material damage.
1.3 Abbreviations used
PCR Polymerase chain reaction
PTFE Polytetrafluorethylen
rcf Relative centrifugal force
rpm Revolutions per
UV Ultraviolet radiation
minute
7
1
3
4
2
1
5
6
7
2
8
9
1
3
4
10
11
2
1
5
7
6
9
8
2
EN
Operating manual
Centrifuge 5424 / 5424 R — Operating manual
2 Product description
2 Product description
2.1 Main illustration
The depiction of the Centrifuge 5424 / 5424 R can also be found on the front fold-out page (see Fig. 1 and Fig. 2).
Abb. 1: Front and rear view of the Ce ntrifuge 5424
Operating manual — Centrifuge 5424 / 5424 Rseeon p.Fig.Tab.p.
Fig. 1: Front and rear view of the Centrifuge 5424
Abb. 2: Front and rear view of the Ce ntrifuge 5424 R
Fig. 2: Front and rear view of the Centrifuge 5424 R
1 Centrifuge lid 2 Monitoring glass
Visual control for rotor stop or option for speed control
via stroboscope.
3Display
Depiction of the current centrifuging parameters and
device settings (see Fig. 4 on p. 19).
4 Control panel
Keys and dials (dependent on the device version) for
operating the centrifuge (see Fig. 3 on p. 18).
5 Emergency lid release
6ID plate
(see Emergency release on p. 32)
7 Mains switch
Switch for switching the device on and off.
Switch position 0: The device is switched off.
Switch position I: The device is switched on.
8 Mains connection
Connection socket for the mains cable supplied.
9 Fuse holder 10 USB port (only 5424 R)
Interface for error analysis and software updates by
the Technical Service.
11 Condensation water tray (only 5424 R)
8
Centrifuge 5424 / 5424 R — Operating manual
2 Product description
2.2 Delivery package
EN
2.2.1 Centrifuge 5424
Quantity Order No.
(International)
1- -
1
or
1 5416 301.001 022634305
1 5703 350.102 022639609
1- - Power cable
1
1
2.2.2 Centrifuge 5424 R
Quantity Order No.
1- -
1
or
1 5416 301.001 022634305
1- - Power cable
1 5404 850.085 5404850085 Tray for condensation water
1
1
5424 852.122
5424 852.130
5404 900.023
5404 900.031
(International)
5424 852.122
5424 852.130
5404 900.023
5404 900.031
Order No.
(North America)
950004266
950004240
5404900023
5404900031
Order No.
(North America)
950004266
950004240
5404900023
5404900031
Description
Centrifuge 5424
See chapter Ordering Information for corresponding device version,
equipment and order number
Fuses
3.15 AT for 230 V, 2 pieces
6.3 AT for 120 V/100 V, 2 pieces
Rotor key
Standard
Captain Eppi rotor key holder
1 piece
Operating manual Centrifuge 5424/5424 R
Languages: EN, DE, FR, ES, IT, PT
Languages: DA, FI, EL, NL, SV (230 V devices only)
Description
Centrifuge 5424 R
See chapter Ordering Information for corresponding device version,
equipment and order number
Fuses
3.15 AT for 230 V, 2 pieces
6.3 AT for 120 V/100 V, 2 pieces
Rotor key
Standard
Operating manual Centrifuge 5424/5424 R
Languages: EN, DE, FR, ES, IT, PT
Languages: DA, FI, EL, NL, SV (230 V devices only)
Operating manual
2.3 Features
The versatile Centrifuge 5424 / 5424 R has a capacity of 24 x 2 mL and reaches a maximum of
21,130 x g / 15,000 rpm (5424: 120 V, 5424 R: 230 V, 120 V, 100 V) or 20,238 x g / 14,680 rpm
(5424: 230 V, 100 V). You can select between four different rotors to centrifuge the following
tubes for your various applications:
• Micro test tubes (0.2 to 2.0 mL)
• PCR strips
• Microtainers (0.6 mL)
• Spin columns (1.5/2.0 mL)
The Centrifuge 5424 R has an additional temperature control function for centrifugation between
-10°C and +40°C. The fast temp function can be used to start a temperature control run without
samples to adjust the rotor chamber quickly to the set target temperature.
9
EN
Centrifuge 5424 / 5424 R — Operating manual
2 Product description
2.4 Rotors
You can operate the Centrifuge 5424 / 5424 R with the following rotors. Before use of sample
tubes, please note the manufacturer's specifications with regard to centrifugation resistance
(max. rcf).
Operating manual
Rotor
FA-45-24-11
Rotor
FA-45-24-11-
Special
Rotor
F-45-18-11-
Kit
Rotor
F-45-32-5-
PCR
Max. capacity Max. g-force (rcf) /
speed (rpm)
without adapter
(5424: 120 V,
5424 R)
Max. load per rotor bore
24 micro test tubes of 1.5/2.0 mL
each or spin columns.
With adapters:
• 0.2 mL PCR tubes
21,130 x g /
15,000 rpm
3.75 g
• 0.4 mL/0.5 mL micro test
tubes
• 0.6 mL Microtainers
24 micro test tubes of 1.5/2.0 mL
each or spin columns.
With adapters:
• 0.2 mL PCR tubes
21,130 x g /
15,000 rpm
3.75 g
• 0.4 mL/0.5 mL micro test
tubes
• 0.6 mL Microtainers
18 spin columns or 1.5/2.0 mL
micro test tubes.
With adapters:
18,111 x g /
15,000 rpm
3.75 g
• 0.2 mL PCR tubes
• 0.4 mL/0.5 mL micro test
tubes
• 0.6 mL Microtainers
Four PCR strips of 5/8 or
32 PCR tubes of 0.2 mL each.
18,615 x g /
15,000 rpm
3.5 g
Max. g-force (rcf) or
speed (rpm)
without adapter
(5424: 230 V/100 V)
(1)
20,238 x g /
14,860 rpm
20,238 x g /
14,860 rpm
17,347 x g /
14,860 rpm
17,829 x g /
14,860 rpm
Notes
• Aerosol-tight
lid (aluminum).
(2)
rotor
• Spin columns
possible, better with
rotor F-45-18-11-Kit.
• Aerosol-tight
lid (aluminum).
(2)
rotor
• PTFE-coated
(particularly resistant
to chemicals),
marked: coated
• Spin columns
possible, better with
rotor F-45-18-11-Kit.
• Particularly high edge
for all commercially
available spin
columns. See the note
about centrifugation
with open tube lids in
this regard(see Load
the rotor on p. 21).
10
(1) Maximum load per rotor bore for adapter + tube + content.
(2) Aerosol tightness tested and certified by the Centre for Emergency Preparedness and
Response, Health Protection Agency, Porton Down (UK) (see certificates at the end of this
operating manual).
For the rotors and rotor lids labeled coated, color fluctuations may occur as a result of the
production process. These fluctuations have no effect on service life or resistance to chemicals.
Centrifuge 5424 / 5424 R — Operating manual
2 Product description
2.4.1 Rcf display and calculation
Use the rpm/rcf key to switch the display of centrifugation speed between speed (rpm) and
g-force (rcf). Ensure that the g-force displayed upon switching is standardized to suit the rotor
FA-45-24-11 without an adapter. When other rotors and adapters are used, you can achieve the
following maximum g-forces (rcf) at the maximum speed (see p. 10):
EN
Operating manual
Rotor Adapters Max. centrifugation
radius r
FA-45-24-11,
FA-45-24-11-Special
F-45-18-11-Kit without adapter 7.2 18,111 17,347
FA-45-32-5-PCR without adapter 7.4 18,615 17,829
without adapter 8.4 21,130 20,238
0.2 mL 6.3 15,848 15,179
0.4 mL 8.4 21,130 20,238
0.5 mL 7.3 18,363 17,558
0.6 mL 8.4 21,130 20,238
0.2 mL 5.1 12,829 12,288
0.4 mL 7.2 18,111 17,347
0.5 mL 6.1 15,345 14,697
0.6 mL 7.2 18,111 17,347
To determine the g-force (rcf) for a specific adapter, you can calculate per DIN 58 970 using the
following formula:
rcf = 1.118 · 10-5 · n2 · r
n: revolutions per minute (rpm)
r
: max. centrifuging radius in cm
max
Example
In rotor FA-45-24-11, the 0.5 mL adapter has a maximum radius of 7.3 cm. At 7,000 rpm a
maximum g-force of 4,000 x g is reached.
max
max
[cm]
Max. g-force (rcf)
(5424 120 V, 5424 R)
Max. g-force (rcf)
(5424 230 V/100 V)
11
EN
Operating manual
3 Safety
3Safety
3.1 Intended use
CAUTION!
3.2 User profile
Centrifuge 5424 / 5424 R — Operating manual
Operating manual — Centrifuge 5424 / 5424 Rseeon p.Fig.Tab.p.
The Centrifuge 5424 / 5424 R is intended exclusively for indoor use and for separating aqueous
solutions and suspensions of various densities in approved test tubes.
Poor safety due to incorrect accessories and spare parts.
The use of accessories and spare parts other than those recommended by Eppendorf may
impair the safety, functioning and precision of the device. Eppendorf cannot be held liable for any
damage resulting from the use of non-recommended accessories and spare parts or from the
improper use of such equipment.
Only use accessories and original spare parts recommended by Eppendorf.
This device may only be operated by correspondingly trained specialist staff. This staff must have
carefully read the operating manual and be familiar with the function of the device.
3.3 Application limits
3.3.1 Declaration concerning the ATEX directive (94/9/EC)
Danger of explosion!
Do not operate the device in areas where work is being carried out with explosive substances.
Do not use this device to process any explosive, radioactive or highly reactive substances.
DANGER!
3.3.2 Maximum service life for accessories
Do not use this device to process any substances which could create an explosive
atmosphere.
Due to its design and the environmental conditions on the inside of the device, the Centrifuge
5424 / 5424 R is not suitable for use in a potentially explosive atmosphere.
The device must therefore only be used in a safe environment, such as in the open environment
of a ventilated laboratory or an extractor hood. The use of substances which may contribute to a
potentially explosive atmosphere is not permitted. The final decision with regard to the risks
connected with the use of such substances is the responsibility of the user.
Risk of injury from chemically or mechanically damaged accessories.
Even small scratches and cracks can lead to serious internal material damage.
Protect all parts from mechanical damage.
CAUTION!
Check accessories regularly.
Do not use rotors or buckets with signs of corrosion or mechanical damage (e.g.
deformations).
Do not use accessories whose maximum service life has been exceeded.
12
Accessory Maximum service life from first
commissioning
Transparent polypropylene rotor lids 3 years
Plastic adapters 1 year
Centrifuge 5424 / 5424 R — Operating manual
3 Safety
For the other rotors and rotor lids of this centrifuge (see Rotors on p. 10) there is no limit for their
service life, as long as the following conditions are met: proper use, recommended maintenance
and undamaged condition. Lids of aerosol-tight rotors must be replaced after 50 autoclave cycles
to ensure aerosol tightness.
The date of manufacture is stamped on the rotors in the format 03/07 (= March 2007) or on the
inside of the plastic rotor lids in the form of a clock . This is for information only and does not
have any reference to the service life.
3.4 Information on product liability
In the following cases, the protection provided by the device may be impaired. The liability for the
function of the device passes to the operator if:
• The device is not used in accordance with the operating manual.
• The device is used outside of the range of application described in the preceding chapters.
• The device is used with accessories or consumables (e.g., tubes) which are not
recommended by Eppendorf.
• The device is maintained or repaired by persons not authorized by Eppendorf.
• The owner has made unauthorized modifications to the device.
EN
Operating manual
3.5 Warnings for intended use
Read the operating manual first and observe the following general safety instructions before
using the Centrifuge 5424 / 5424 R.
3.5.1 Personal injury or damage to the equipment
Risk of electrical shock from damage to the device or mains cable.
The device may only be switched on if the device and mains cable are undamaged.
Only use devices which have been professionally installed or repaired.
WARNING!
Lethal voltages inside the device.
Ensure that the housing is always closed and undamaged so that no parts inside the device
can be contacted by accident.
WARNING!
WARNING!
Do not remove the housing of the device.
Do not allow any liquids to enter the inside of the housing.
Do not allow the device to be opened by anyone except service personnel who have been
specifically authorized by Eppendorf.
Danger from using an incorrect power supply.
Only connect the device to voltage supplies which correspond with the electrical requirements
on the name plate.
13
EN
Operating manual
3 Safety
WARNING!
WARNING!
Centrifuge 5424 / 5424 R — Operating manual
Damage to health when handling infectious liquids and pathogenic germs.
Observe the national regulations for handling these substances, the biological security level
of your laboratory, the material safety data sheets and the manufacturer's application notes.
Use suitable aerosol-tight closure systems for the centrifugation of these substances.
When working with pathogenic germs belonging to a higher risk group, more than one
aerosol-tight bioseal must be provided for.
Wear personal protective equipment (PPE).
Follow the instructions regarding hygiene, cleaning and decontamination.
For complete instructions regarding the handling of germs or biological material of risk group
II or higher, please refer to the "Laboratory Biosafety Manual" (Source: World Health
Organization, current edition of the Laboratory Biosafety Manual).
Centrifuge lid can crush. Keep hands clear.
When opening or closing the device lid, do not reach between the lid and device or into the
latching mechanism of the lid.
Always open the centrifuge lid completely to prevent it from falling.
Damage to device by spilling liquids in the rotor or rotor chamber
1. Switch the device off.
2. Disconnect the device from the power supply.
NOTICE!
NOTICE!
3. Clean the device and the accessories carefully in accordance with the cleaning and
disinfection instructions in the operating manual.
4. If a different cleaning and disinfecting method is to be used, contact Eppendorf to ensure that
the intended method will not damage the device.
Damage to electronic components through formation of condensation.
After the device has been moved from a cool to a warmer environment, condensation can form
inside the device.
Wait at least three hours (5424) or four hours (5424 R) before connecting the device to the
power supply.
Only 5424: Alternatively: let the device run for half an hour before transporting it.
3.5.2 Incorrect handling of the centrifuge
Damage from knocking against or moving the device during operation.
A rotor banging against the rotor chamber wall can cause considerable damage to the device and
rotor.
NOTICE!
Do not move or knock against the device during operation.
3.5.3 Incorrect handling of the rotors
Risk of injury from improperly attached rotors and rotor lids.
Centrifuge only with the rotor and rotor lid firmly tightened.
If unusual noises occur when the centrifuge starts, the rotor or the rotor lid may not be
CAUTION!
14
properly secured. Stop centrifugation immediately by pressing the start/stop key.
Loading...