Eppendorf 5424 R User Manual [en, es]

5404 900.023-02/0810

Centrifuge 5424/5424 R

Operating manual

Manual de instrucciones

Trademarks

eppendorf is a registered trademark of Eppendorf AG, Hamburg, Germany. Microtainer is a registered trademark of Becton Dickinson, Franklin Lakes, NJ, USA.

Trademarks are not marked in all cases with ™ or

in this manual.

Your Online Product Registration Register your Eppendorf instruments online and get rewarded! Learn more: www.eppendorf.com/myeppendorf

Not available in all countries.

Operating manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

Manual de instrucciones. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 99

Declarations and Certificates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 176

5404 900.023-04/122011

You will find a detailed description of these figures in your language in Chapters 1.1/2.1 and 4.1/5.1.

6 7

3 4

9 8

Abb. 1: Front and rear view of the Ce ntrifuge 5424

Fig. 1: Front and rear view of the Centrifuge 5424

Abb. 2: Front and rear view of the Ce ntrifuge 5424 R

Fig. 2: Front and rear view of the Centrifuge 5424 R

1 Centrifuge lid 2 Window

3 Display 4 Control panel

5 Emergency release 6 ID plate

7 Mains switch 8 Power connection

9 Fuse holder 10 USB port

(only 5424 R, only for technical support)

11 Condensation water tray (only 5424 R)

Abb. 3: Control panel of the Centr ifuge 5424 / 5424 R

Fig. 3: Control panel of the Centrifuge 5424 / 5424 R

1 Call and select the menu parameters 2 Switch the centrifuging speed displayed (rpm/rcf)

3 Display 4 Short Spin centrifugation

5 Release lid 6 Start and stop centrifugation

7 Set the speed of centrifugation

8 Adjust the temperature (only 5424 R)

Dependent on device version designed as a key or dial.

9 Adjust the centrifuging duration

Dependent on device version designed as a key or

10 Start the temperature control run fast temp 

(only 5424 R)

dial.

11 Select the menu item

Abb. 4: Display of the Centrifug e 5424 / 5424 R

Fig. 4: Display of the Centrifuge 5424 R. The display of the Centrifuge 5424 is similar.

1 Centrifuging duration 2 Status of the key lock (LOCK)

3 Status of the function ATSET 4 Temperature (only 5424 R)

5 Soft ramp 6 g-force (rcf) or speed (rpm)

7 Status of the centrifuge 8 Status of the centrifugation speed display

9 Status of the loudspeaker

Centrifuge 5424 / 5424 R — Operating manual

Operating manual

Table of contents

1 User instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

1.1 Using this manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

1.2 Warning signs and hazard icons. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

1.2.1 Hazard symbols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

1.2.2 Degrees of danger . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

1.3 Abbreviations used. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

2 Product description. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

2.1 Main illustration. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

2.2 Delivery package . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

2.2.1 Centrifuge 5424 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

2.2.2 Centrifuge 5424 R. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

2.3 Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

2.4 Rotors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

2.4.1 Rcf display and calculation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

3 Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

3.1 Intended use. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

3.2 User profile . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

3.3 Application limits. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

3.3.1 Declaration concerning the ATEX directive (94/9/EC). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

3.3.2 Maximum service life for accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

3.4 Information on product liability . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

3.5 Warnings for intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

3.5.1 Personal injury or damage to the equipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

3.5.2 Incorrect handling of the centrifuge. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

3.5.3 Incorrect handling of the rotors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

3.5.4 Extreme strain on the centrifuging tubes. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

3.5.5 Aerosol-tight centrifugation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16

3.6 Safety instructions on the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16

4 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17

4.1 Selecting the location . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17

4.2 Installing the instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17

5 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18

5.1 Overview of operating controls. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18

5.2 Device menu. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

5.3 Menu navigation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21

5.4 Preparing for centrifugation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21

5.4.1 Switch on centrifuge . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21

5.4.2 Inserting the rotor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21

5.4.3 Load the rotor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21

5.4.4 Close centrifuge lid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23

5.5 Cooling (only 5424 R). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23

5.5.1 Temperature adjustment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23

5.5.2 Temperature display . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23

5.5.3 Temperature monitoring . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23

5.5.4 Fast Temp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23

5.5.5 Continuous cooling . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24

5.6 Centrifuging . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24

5.6.1 Centrifuging with time preset. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

5.6.

2 Centrifuging in continuous operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

5.6.3 Short spin centrifugation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26

5.6.4 Removing the rotor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26

Operating manual — Centrifuge 5424 / 5424 Rseeon p.Fig.Tab.p.

. . . . . . . . 25

Operating manual

Centrifuge 5424 / 5424 R — Operating manual

Table of contents

6 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27

6.1 Prepare cleaning / disinfection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27

6.2 Perform cleaning / disinfection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27

6.2.1 Clean / disinfect device. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28

6.2.2 Clean / disinfect rotor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28

6.3 Additional service instructions for Centrifuge 5424 R. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29

6.4 Glass breakage. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29

6.5 Fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29

6.6 Decontamination before shipping . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29

7 Troubleshooting. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30

7.1 General errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30

7.2 Error messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31

7.3 Emergency release. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32

8 Transport, storage and disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

8.1 Transport . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

8.2 Storage. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

8.3 Disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

9 Technical data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34

9.1 Power supply . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34

9.2 Ambient conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34

9.3 Weight / dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35

9.4 Application parameters. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35

9.4.1 Centrifuge 5424 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35

9.4.2 Centrifuge 5424 R. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36

10 Ordering information. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37

10.1 Centrifuge 5424 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37

10.2 Centrifuge 5424 R . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37

10.3 Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38

10.3.1 Rotors and rotor lids . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38

10.3.2 Adapters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38

10.3.3 Other accessories. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38

10.4 Fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38

Centrifuge 5424 / 5424 R — Operating manual

1 User instructions

1.1 Using this manual

1.2 Warning signs and hazard icons

1.2.1 Hazard symbols

Operating manual — Centrifuge 5424 / 5424 Rseeon p.Fig.Tab.p.

Please read this operating manual completely before using the device for the first time.

  Please view this operating manual as part of the product and keep it somewhere easily

accessible.

 When passing the device on to third parties, be sure to include this operating manual.  If this manual is lost, please request another one. The current version can be found on our

website www.eppendorf.com.

The Centrifuge 5424 / 5424 R is available in two versions: keypad or rotary knobs. This operating manual generally describes how to operate the keypad version. However, it also applies to the rotary knob version.

Biohazard Explosion

Operating manual

Electric shock Crushing

Hazard point Material damage

1.2.2 Degrees of danger

The degree of danger is a part of a safety note and distinguishes the possible results of non-observance from each other.

DANGER Will lead to severe injuries or death.

WARNING May lead to severe injuries or death.

CAUTION May lead to light to moderate injuries.

NOTICE May lead to material damage.

1.3 Abbreviations used

PCR Polymerase chain reaction

PTFE Polytetrafluorethylen

rcf Relative centrifugal force

rpm Revolutions per

UV Ultraviolet radiation

minute

6 7

3 4

9 8

Operating manual

Centrifuge 5424 / 5424 R — Operating manual

2 Product description

2.1 Main illustration

The depiction of the Centrifuge 5424 / 5424 R can also be found on the front fold-out page (see Fig. 1 and Fig. 2).

Abb. 1: Front and rear view of the Ce ntrifuge 5424

Operating manual — Centrifuge 5424 / 5424 Rseeon p.Fig.Tab.p.

Fig. 1: Front and rear view of the Centrifuge 5424

Abb. 2: Front and rear view of the Ce ntrifuge 5424 R

Fig. 2: Front and rear view of the Centrifuge 5424 R

1 Centrifuge lid 2 Monitoring glass

Visual control for rotor stop or option for speed control via stroboscope.

3Display

Depiction of the current centrifuging parameters and device settings (see Fig. 4 on p. 19).

4 Control panel

Keys and dials (dependent on the device version) for operating the centrifuge (see Fig. 3 on p. 18).

5 Emergency lid release

6ID plate

(see Emergency release on p. 32)

7 Mains switch

Switch for switching the device on and off. Switch position 0: The device is switched off. Switch position I: The device is switched on.

8 Mains connection

Connection socket for the mains cable supplied.

9 Fuse holder 10 USB port (only 5424 R)

Interface for error analysis and software updates by the Technical Service.

11 Condensation water tray (only 5424 R)

Centrifuge 5424 / 5424 R — Operating manual

2 Product description

2.2 Delivery package

2.2.1 Centrifuge 5424

Quantity Order No.

(International)

1- -

1 or

1 5416 301.001 022634305

1 5703 350.102 022639609 1- - Power cable

1 1

2.2.2 Centrifuge 5424 R

Quantity Order No.

1- -

1 or

1 5416 301.001 022634305 1- - Power cable 1 5404 850.085 5404850085 Tray for condensation water

1 1

5424 852.122 5424 852.130

5404 900.023 5404 900.031

(International)

5424 852.122 5424 852.130

5404 900.023 5404 900.031

Order No. (North America)

950004266 950004240

5404900023 5404900031

Order No. (North America)

950004266 950004240

5404900023 5404900031

Description

Centrifuge 5424

See chapter Ordering Information for corresponding device version, equipment and order number

Fuses

3.15 AT for 230 V, 2 pieces

6.3 AT for 120 V/100 V, 2 pieces

Rotor key

Standard

Captain Eppi rotor key holder

1 piece

Operating manual Centrifuge 5424/5424 R

Languages: EN, DE, FR, ES, IT, PT Languages: DA, FI, EL, NL, SV (230 V devices only)

Description

Centrifuge 5424 R

See chapter Ordering Information for corresponding device version, equipment and order number

Fuses

3.15 AT for 230 V, 2 pieces

6.3 AT for 120 V/100 V, 2 pieces

Rotor key

Standard

Operating manual Centrifuge 5424/5424 R

Languages: EN, DE, FR, ES, IT, PT Languages: DA, FI, EL, NL, SV (230 V devices only)

Operating manual

2.3 Features

The versatile Centrifuge 5424 / 5424 R has a capacity of 24 x 2 mL and reaches a maximum of 21,130 x g / 15,000 rpm (5424: 120 V, 5424 R: 230 V, 120 V, 100 V) or 20,238 x g / 14,680 rpm (5424: 230 V, 100 V). You can select between four different rotors to centrifuge the following tubes for your various applications:

• Micro test tubes (0.2 to 2.0 mL)

• PCR strips

• Microtainers (0.6 mL)

• Spin columns (1.5/2.0 mL)

The Centrifuge 5424 R has an additional temperature control function for centrifugation between

-10°C and +40°C. The fast temp function can be used to start a temperature control run without samples to adjust the rotor chamber quickly to the set target temperature.

Centrifuge 5424 / 5424 R — Operating manual

2 Product description

2.4 Rotors

You can operate the Centrifuge 5424 / 5424 R with the following rotors. Before use of sample tubes, please note the manufacturer's specifications with regard to centrifugation resistance (max. rcf).

Operating manual

Rotor FA-45-24-11

Rotor FA-45-24-11-

Special

Rotor F-45-18-11-

Kit

Rotor F-45-32-5-

PCR

Max. capacity Max. g-force (rcf) /

speed (rpm) without adapter (5424: 120 V, 5424 R)

Max. load per rotor bore

24 micro test tubes of 1.5/2.0 mL each or spin columns.

With adapters:

• 0.2 mL PCR tubes

21,130 x g / 15,000 rpm

3.75 g

• 0.4 mL/0.5 mL micro test

tubes

• 0.6 mL Microtainers

24 micro test tubes of 1.5/2.0 mL each or spin columns.

With adapters:

• 0.2 mL PCR tubes

21,130 x g / 15,000 rpm

3.75 g

• 0.4 mL/0.5 mL micro test

tubes

• 0.6 mL Microtainers

18 spin columns or 1.5/2.0 mL micro test tubes.

With adapters:

18,111 x g / 15,000 rpm

3.75 g

• 0.2 mL PCR tubes

• 0.4 mL/0.5 mL micro test

tubes

• 0.6 mL Microtainers

Four PCR strips of 5/8 or  32 PCR tubes of 0.2 mL each.

18,615 x g / 15,000 rpm

3.5 g

Max. g-force (rcf) or speed (rpm) without adapter (5424: 230 V/100 V)

(1)

20,238 x g / 14,860 rpm

17,347 x g / 14,860 rpm

17,829 x g / 14,860 rpm

Notes

• Aerosol-tight

lid (aluminum).

(2)

rotor

• Spin columns

possible, better with rotor F-45-18-11-Kit.

• Aerosol-tight

lid (aluminum).

(2)

rotor

• PTFE-coated

(particularly resistant to chemicals), marked: coated

• Spin columns

possible, better with rotor F-45-18-11-Kit.

• Particularly high edge

for all commercially available spin columns. See the note about centrifugation with open tube lids in this regard(see Load the rotor on p. 21).

(1) Maximum load per rotor bore for adapter + tube + content. (2) Aerosol tightness tested and certified by the Centre for Emergency Preparedness and

Response, Health Protection Agency, Porton Down (UK) (see certificates at the end of this operating manual).

For the rotors and rotor lids labeled coated, color fluctuations may occur as a result of the production process. These fluctuations have no effect on service life or resistance to chemicals.

Centrifuge 5424 / 5424 R — Operating manual

2 Product description

2.4.1 Rcf display and calculation

Use the rpm/rcf key to switch the display of centrifugation speed between speed (rpm) and g-force (rcf). Ensure that the g-force displayed upon switching is standardized to suit the rotor

FA-45-24-11 without an adapter. When other rotors and adapters are used, you can achieve the following maximum g-forces (rcf) at the maximum speed (see p. 10):

Operating manual

Rotor Adapters Max. centrifugation

radius r

FA-45-24-11, FA-45-24-11-Special

F-45-18-11-Kit without adapter 7.2 18,111 17,347

FA-45-32-5-PCR without adapter 7.4 18,615 17,829

without adapter 8.4 21,130 20,238

0.2 mL 6.3 15,848 15,179

0.4 mL 8.4 21,130 20,238

0.5 mL 7.3 18,363 17,558

0.6 mL 8.4 21,130 20,238

0.2 mL 5.1 12,829 12,288

0.4 mL 7.2 18,111 17,347

0.5 mL 6.1 15,345 14,697

0.6 mL 7.2 18,111 17,347

To determine the g-force (rcf) for a specific adapter, you can calculate per DIN 58 970 using the following formula:

rcf = 1.118 · 10-5 · n2 · r n: revolutions per minute (rpm) r

: max. centrifuging radius in cm

max

Example

In rotor FA-45-24-11, the 0.5 mL adapter has a maximum radius of 7.3 cm. At 7,000 rpm a maximum g-force of 4,000 x g is reached.

max

[cm]

Max. g-force (rcf) (5424 120 V, 5424 R)

Max. g-force (rcf) (5424 230 V/100 V)

Operating manual

3 Safety

3Safety

3.1 Intended use

CAUTION!

3.2 User profile

Centrifuge 5424 / 5424 R — Operating manual

Operating manual — Centrifuge 5424 / 5424 Rseeon p.Fig.Tab.p.

The Centrifuge 5424 / 5424 R is intended exclusively for indoor use and for separating aqueous solutions and suspensions of various densities in approved test tubes.

Poor safety due to incorrect accessories and spare parts.

The use of accessories and spare parts other than those recommended by Eppendorf may impair the safety, functioning and precision of the device. Eppendorf cannot be held liable for any damage resulting from the use of non-recommended accessories and spare parts or from the improper use of such equipment.

 Only use accessories and original spare parts recommended by Eppendorf.

This device may only be operated by correspondingly trained specialist staff. This staff must have carefully read the operating manual and be familiar with the function of the device.

3.3 Application limits

3.3.1 Declaration concerning the ATEX directive (94/9/EC)

Danger of explosion!

 Do not operate the device in areas where work is being carried out with explosive substances.  Do not use this device to process any explosive, radioactive or highly reactive substances.

DANGER!

3.3.2 Maximum service life for accessories

 Do not use this device to process any substances which could create an explosive

atmosphere.

Due to its design and the environmental conditions on the inside of the device, the Centrifuge 5424 / 5424 R is not suitable for use in a potentially explosive atmosphere.

The device must therefore only be used in a safe environment, such as in the open environment of a ventilated laboratory or an extractor hood. The use of substances which may contribute to a potentially explosive atmosphere is not permitted. The final decision with regard to the risks connected with the use of such substances is the responsibility of the user.

Risk of injury from chemically or mechanically damaged accessories.

Even small scratches and cracks can lead to serious internal material damage.

 Protect all parts from mechanical damage.

CAUTION!

 Check accessories regularly.  Do not use rotors or buckets with signs of corrosion or mechanical damage (e.g.

deformations).

 Do not use accessories whose maximum service life has been exceeded.

Accessory Maximum service life from first

commissioning

Transparent polypropylene rotor lids 3 years

Plastic adapters 1 year

Centrifuge 5424 / 5424 R — Operating manual

3 Safety

For the other rotors and rotor lids of this centrifuge (see Rotors on p. 10) there is no limit for their service life, as long as the following conditions are met: proper use, recommended maintenance and undamaged condition. Lids of aerosol-tight rotors must be replaced after 50 autoclave cycles to ensure aerosol tightness.

The date of manufacture is stamped on the rotors in the format 03/07 (= March 2007) or on the inside of the plastic rotor lids in the form of a clock . This is for information only and does not have any reference to the service life.

3.4 Information on product liability

In the following cases, the protection provided by the device may be impaired. The liability for the function of the device passes to the operator if:

• The device is not used in accordance with the operating manual.

• The device is used outside of the range of application described in the preceding chapters.

• The device is used with accessories or consumables (e.g., tubes) which are not

recommended by Eppendorf.

• The device is maintained or repaired by persons not authorized by Eppendorf.

• The owner has made unauthorized modifications to the device.

Operating manual

3.5 Warnings for intended use

Read the operating manual first and observe the following general safety instructions before using the Centrifuge 5424 / 5424 R.

3.5.1 Personal injury or damage to the equipment

Risk of electrical shock from damage to the device or mains cable.

 The device may only be switched on if the device and mains cable are undamaged.  Only use devices which have been professionally installed or repaired.

WARNING!

Lethal voltages inside the device.

 Ensure that the housing is always closed and undamaged so that no parts inside the device

can be contacted by accident.

WARNING!

 Do not remove the housing of the device.  Do not allow any liquids to enter the inside of the housing.  Do not allow the device to be opened by anyone except service personnel who have been

specifically authorized by Eppendorf.

Danger from using an incorrect power supply.

 Only connect the device to voltage supplies which correspond with the electrical requirements

on the name plate.

Operating manual

3 Safety

WARNING!

Centrifuge 5424 / 5424 R — Operating manual

Damage to health when handling infectious liquids and pathogenic germs.

 Observe the national regulations for handling these substances, the biological security level

of your laboratory, the material safety data sheets and the manufacturer's application notes.

 Use suitable aerosol-tight closure systems for the centrifugation of these substances.  When working with pathogenic germs belonging to a higher risk group, more than one

aerosol-tight bioseal must be provided for.

 Wear personal protective equipment (PPE).  Follow the instructions regarding hygiene, cleaning and decontamination.  For complete instructions regarding the handling of germs or biological material of risk group

II or higher, please refer to the "Laboratory Biosafety Manual" (Source: World Health Organization, current edition of the Laboratory Biosafety Manual).

Centrifuge lid can crush. Keep hands clear.

 When opening or closing the device lid, do not reach between the lid and device or into the

latching mechanism of the lid.

 Always open the centrifuge lid completely to prevent it from falling.

Damage to device by spilling liquids in the rotor or rotor chamber

1. Switch the device off.

2. Disconnect the device from the power supply.

NOTICE!

3. Clean the device and the accessories carefully in accordance with the cleaning and disinfection instructions in the operating manual.

4. If a different cleaning and disinfecting method is to be used, contact Eppendorf to ensure that the intended method will not damage the device.

Damage to electronic components through formation of condensation.

After the device has been moved from a cool to a warmer environment, condensation can form inside the device.

 Wait at least three hours (5424) or four hours (5424 R) before connecting the device to the

power supply.

 Only 5424: Alternatively: let the device run for half an hour before transporting it.

3.5.2 Incorrect handling of the centrifuge

Damage from knocking against or moving the device during operation.

A rotor banging against the rotor chamber wall can cause considerable damage to the device and rotor.

NOTICE!

 Do not move or knock against the device during operation.

3.5.3 Incorrect handling of the rotors

Risk of injury from improperly attached rotors and rotor lids.

 Centrifuge only with the rotor and rotor lid firmly tightened.  If unusual noises occur when the centrifuge starts, the rotor or the rotor lid may not be

CAUTION!

properly secured. Stop centrifugation immediately by pressing the start/stop key.

3 Safety

CAUTION!

NOTICE!

Centrifuge 5424 / 5424 R — Operating manual

Risk of injury from asymmetric loading of rotors.

 Load rotors symmetrically with identical tubes.  Only load adapters with suitable tubes.  Always use tubes of the same type (weight, material/density and volume).  Check symmetric loading by balancing the adapters and tubes used with scales.

Risk of injury from overloaded rotor.

The Centrifuge 5424 / 5424 R is designed for the centrifugation of material with a max. density of

1.2 g/mL at maximum speed and volume and/or load.

 Observe the information on each rotor relating to maximum load (adapter, tube and contents)

per rotor bore and make sure it is not exceeded.

Damage to rotors from aggressive chemicals.

Rotors are high-quality components which withstand extreme stresses. This stability can be impaired by aggressive chemicals.

 Avoid the use of aggressive chemicals, including strong and weak alkali, strong acids,

solutions with mercury, copper and other heavy metal ions, halogenated hydrocarbons, concentrated saline solutions and phenol.

 If the rotor is contaminated by aggressive chemicals, clean it immediately using a neutral

cleaning agent. This applies to the rotor bores in particular.

Operating manual

3.5.4 Extreme strain on the centrifuging tubes

Risk of injury from overloaded tubes.

 Note the loading limits specified by the tube manufacturer.  Only use tubes which are approved by the manufacturer for the required rcf.

CAUTION!

Risk from damaged tubes.

Damaged tubes must not be used, as this could cause further damage to the device and the accessories and loss of the samples.

NOTICE!

 Before use, carry out a visual check of all tubes for any damage.

Risk from open tube lids.

Open tube lids can break off during centrifugation and cause damage to the rotor as well as to the centrifuge.

 Carefully close all tube lids before centrifuging.

Exception: Note the information on the centrifugation of spin columns in the rotor F-45-18-11-Kit (see Load the rotor on p. 21).

Hazard to plastic tubes from organic solvents.

When using organic solvents (e.g. phenol, chloroform) the density of plastic tubes is reduced, i.e., the tubes could get damaged.

 Follow the manufacturer's information about the chemical resistance of tubes.

Centrifuge 5424 / 5424 R — Operating manual

3 Safety

Sample tubes heat up.

In uncooled centrifuges the temperature in the rotor chamber, rotor and sample can rise to above 40°C dependent on the run time, g-force (rcf) or speed (rpm) and ambient temperature.

NOTICE!

3.5.5 Aerosol-tight centrifugation

 Note that this can reduce the centrifugation resistance of the sample tubes.  Please note the temperature resistance of the samples.

Operating manual

Risk to health due to limited aerosol tightness with incorrect rotor/rotor lid combination.

Aerosol-tight centrifugation is guaranteed only if the rotors and rotor lids intended for this purpose are used. These are always indicated by the prefix FA.

WARNING!

3.6 Safety instructions on the device

Depiction Meaning Location

 Always use rotors and rotor lids marked aerosol-tight together for aerosol-tight centrifugation.  Only use aerosol-tight rotor lids in combination with rotors which are marked on the rotor lid.

Risk to health due to limited aerosol tightness in the event of incorrect use.

Autoclaving, mechanical stresses and contamination by chemicals or other aggressive solvents can impair the aerosol-tightness of the rotors and rotor lid.

 Regularly check that the seals of aerosol-tight rotor lids are undamaged.  Only use aerosol-tight rotor lids with undamaged and clean gaskets.  Thinly brush the threads of the rotor lid screw with pivot grease (order no. Int.: 5810 350.050 /

North America: 022634330) after every proper autoclaving (121°C, 20 min.). Do not apply the pivot grease to the gaskets.

 Replace aerosol-tight rotor lids after 50 autoclave cycles.  Aerosol-tight rotors should never be stored with rotor lids screwed on.

WARNING

General hazard point. Follow the operating manual.

Right side of the device

CAUTION

Always tighten up the rotor using the rotor key supplied.

CAUTION

Close all tubes and use a rotor lid.

Top of device, below the centrifuge lid.

4 Installation

4Installation

4.1 Selecting the location

Operating manual — Centrifuge 5424 / 5424 Rseeon p.Fig.Tab.p.

Centrifuge 5424 / 5424 R — Operating manual

If a fault occurs, objects in the immediate vicinity of the devices could get damaged.

 In accordance with the recommendations of EN 61010-2-020, leave a safety distance of 30

cm clear around the device during operation.

NOTICE!

Damage from overheating.

 Do not place the device close to sources of heat (e.g., radiator, drying cabinet).  Do not expose the device to direct sunlight.

NOTICE!

 Ensure free circulation of air by maintaining a distance of at least 30 cm on all sides of the

device from adjacent devices or the wall and keep the underside of the device clear.

 Make sure that the vents in the device are always free of obstruction.

Select the location for the device according to the following criteria:

• Suitable power connection as per the name plate (230 V/120 V/100 V).

• Stable, horizontal and resonance-free lab bench. Weight of the device: 13.4 kg (5424) or

21 kg (5424 R).

• A well ventilated environment which is protected from direct sunlight to prevent the device

from heating up more.

4.2 Installing the instrument

Centrifuge 5424 R: Compressor damage after improper transport.

 Only switch on the centrifuge 4 hours after installation.

Operating manual

NOTICE!

Hint!

Perform the following steps in the sequence described.

1. Place the device on a suitable lab bench.

2. Allow the device to warm up for at least 3 hours (5424) or 4 hours (5424 R) to the ambient temperature to prevent damage to the electronic components from condensation and damage to the compressor (only5424 R).

3. Check that the mains voltage and frequency match the requirements on the device type plate.

4. Connect the centrifuge to the mains and switch it on using the mains power switch on the right side of the device.

• The display is active.

• Lid opens automatically

5. Use the details included in the scope of delivery to check that the delivery is complete.

6. Check all parts for any transport damage. Contact your dealer if any damage is found.

7. Only 5424 R: Insert the condensation water tray at the left side of the device into the holder provided (see Fig. 2 on p. 8).

Retain the packaging material and the transport protection device for subsequent transport or storage. See also the instructions relating to transport (see p. 33).

Operating manual

Centrifuge 5424 / 5424 R — Operating manual

5 Operation

5Operation

5.1 Overview of operating controls

Abb. 3: Control panel of the Centr ifuge 5424 / 5424 R

Operating manual — Centrifuge 5424 / 5424 Rseeon p.Fig.Tab.p.

Before using the Centrifuge 5424 / 5424 R for the first time, familiarize yourself with the operating controls and the display.

The depiction of the operating controls and the display can also be found on the front fold-out page (see Fig. 3 and Fig. 4).

Fig. 3: Control panel of the Centrifuge 5424 / 5424 R

1 Call and select the menu parameters

(see Device menu on p. 20)

3 Display 4 Short spin centrifugation

5 Release lid 6 Start and stop centrifugation

7 Set the speed of centrifugation

Dependent on device version designed as a key or dial.

9 Adjust the centrifuging duration

Dependent on device version designed as a key or dial.

11 Select the menu item

(see Menu navigation on p. 21)

Please also read the precise description of the individual menu functions (see p. 20).

2 Switch the displayed centrifuging speed (rpm/rcf)

(see Rcf display and calculation on p. 11)

(see Short spin centrifugation on p. 26)

8 Adjust the temperature (only 5424 R)

10 Start the temperature control run fast temp 

(only 5424 R)

5 Operation

Centrifuge 5424 / 5424 R — Operating manual

Abb. 4: Display of the Centrifug e 5424 / 5424 R

Fig. 4: Display of the Centrifuge 5424 R. The display of the Centrifuge 5424 is similar.

1 Centrifuging duration 2 Status of the key lock (LOCK)

: Centrifuging parameters cannot be modified

unintentionally.

: No key lock.

3 Status of the function ATSET

: Start of operation when reaching 95% of the

preset g-force (rcf) or speed (rpm).

: Start of operation immediately.

5 Soft ramp

: Rotor accelerates and brakes slowly.

No symbol: Rotor accelerates and brakes rapidly.

4 Temperature (only 5424 R)

6 g-force (rcf) or speed (rpm)

(see Rcf display and calculation on p. 11)

Operating manual

7 Status of the centrifuge

: Centrifuge lid unlocked.

: Centrifuge lid locked.

(Flashing): centrifuging in progress.

5 Soft ramp

: Rotor accelerates and brakes slowly.

No symbol: Rotor accelerates and brakes rapidly.

9 Status of the loudspeaker

: Switched on

: Switched off

8 Status of the centrifugation speed display

rcf: g-force (relative centrifugal force)

rpm: revolutions per minute

6 g-force (rcf) or speed (rpm)

(see Rcf display and calculation on p. 11)

Centrifuge 5424 / 5424 R — Operating manual

5 Operation

5.2 Device menu

Tab. 1: Menu structure of the Centrifuge 5424 / 5424 R. All menu levels contain the additional menu item Back.

Operating manual

Level 1 (M I) Level 2

(M 2)

SOFT

Soft ramp: Reduce speed of acceleration and braking ramp. Not used for short spin centrifugation.

LOCK

Key lock: Set the current centrifugation parameters permanently to prevent the time, temperature (only 5424 R) g-force (rcf) or speed (rpm) from being unintentionally modified.

ATSET

Set start of centrifuging run time.

SHORT

Before the start of a short run (see Short spin centrifugation on p. 26) it is possible to switch

between the maximum and currently set g-force (rcf) or speed (rpm). The SOFT function is not used for short spin centrifugation.

On Rotor accelerates and brakes slowly.

Off Rotor accelerates and brakes rapidly.

On Set the centrifugation parameters permanently.

Off

On The set time is counted down only once 95% of

Off The set time is counted down immediately.

MAX Short spin run at maximum g-force (rcf) or

Set Short run at set g-force (rcf) or speed (rpm).

Function Display

When you select the keys time, temp (only 5424 R) and speed, the display shows SAFE.

the specified g-force (rcf) or speed (rpm) has been reached.

speed (rpm) of the rotor used.

TEMP (only 5424 R) Set the time limitation of continuous cooling

(see p. 24).

ALARM On Switch on loudspeaker.

VOL VOL1

SLEEP

Standby mode

LID (only 5424) Lid release, from software version 2.2.

8 h Preset value.

oo Endless operation of continuous cooling.

Icing possible! Note that this may reduce the service life of the compressor.

Off Switch off loudspeaker.

Adjust the speaker volume in 5 steps. The loud-

...

VOL5

On If the centrifuge has not been used for 15 min and

Off Standby mode deactivated.

AUTO The centrifuge lid opens automatically after

Off The centrifuge lid must be manually opened with

speaker must be switched on for the adjustment to be audible.

the standby mode has been switched on, it switches to standby mode. The EP logo then appears in the display. When a button or knob is used or the centrifuge lid is closed, the centrifuge is reactivated. It is then ready for operation.

centrifugation.

the open key after centrifugation.

5 Operation

5.3 Menu navigation

To change settings in the device menu, proceed as follows:

1. Open the menu.

2. Select the desired menu item.

3. Confirm your selection.

4. Select the setting of the parameters in question.

Centrifuge 5424 / 5424 R — Operating manual

Operating manual

6. Exit the menu.

To exit the second menu level without changing a parameter, select the BACK menu item and confirm with menu/enter.

Hint!

Confirm the changed setting. The BACK menu item belonging to the first menu level appears.

5.4 Preparing for centrifugation

5.4.1 Switch on centrifuge

1. Switch the centrifuge on, using the mains switch. After switching on at the mains power switch, the centrifuge lid opens automatically.

2. Open the closed centrifuge lid by pressing the open key. The parameter settings of the last run are displayed.

5.4.2 Inserting the rotor

1. Place rotor vertically onto the motor shaft.

2. Insert the rotor key supplied into the rotor nut.

3. Turn rotor key clockwise until the rotor nut is firmly tightened.

5.4.3 Load the rotor

CAUTION!

Risk of injury from asymmetric loading of rotors.

Operating manual

5 Operation

CAUTION!

Hint!

Centrifuge 5424 / 5424 R — Operating manual

Risk from damaged or overloaded tubes!

 When loading the rotor, note the safety instructions with regard to hazards from overloaded or

damaged tubes(see Warnings for intended use on p. 13)

Rotor lid!

• Fixed-angle rotors may only be operated with the appropriate rotor lid in each case. This is

clearly shown by the identical rotor name labeling on the rotor and on the rotor lid.

• To carry out an aerosol-tight centrifugation, an aerosol-tight rotor (marking: red ring) and the

corresponding aerosol-tight rotor lid (marking: aerosol-tight and red lid screw) must be used.

To load the rotor, proceed as follows:

1. Check the maximum load (adapter, tube and content) per rotor bore. The information about this can be found on every rotor and in this operating manual

(see Rotors on p. 10).

2. Load rotors and adapters only with the tubes intended for them.

3. Insert tubes opposite each other in pairs into the rotor bores. For symmetrical loading, tubes that are opposite each other must be of the same type and contain the same filling quantity.

In order to minimize weight differences between filled sample tubes, we recommend taring with a scale. This will reduce wear on the drive and cut running noise.

4. Attach and tighten rotor lid.

With the rotors FA-45-24-11 and FA-45-24-11-Special centrifugation is also possible without a rotor lid.

Hint!

Please also note:

• The tube lids must be closed securely.

• The rotors are not aerosol-tight without rotor lid.

• The centrifugation is slightly louder.

• Spin columns must always be centrifuged with a rotor lid.

Spin columns

Hint!

For centrifuging spin columns in the rotor F-45-18-11-Kit, you can leave the tube lids open. However, this is possible only using the tubes provided for this purpose by kit manufacturers. For reliable centrifugation, you must lean the open tube lids against the edge of the rotor. Ensure that this does not involve the lids projecting beyond the edge of the rotor, and then put on the associated rotor lid.

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