BIO RAD NGC Quick Manual

CHROMATOGRAPHY SYSTEMS

NGC™ Fraction Collector Installation

Quick Guide

Installing the NGC Fraction Collector

Introduction

The NGC Fraction Collector provides versatile options for
preparative and analytical chromatography applications. The
high-capacity and flexible rack options enable fractions ranging
in size from milliliters to liters to be collected and accessed
freely during a purification run.

Setting Up the NGC Fraction Collector

This quick guide outlines the process of installing and plumbing
the NGC Fraction Collector to your NGC System. The fraction
collector (FC) can be installed on a laboratory bench or in a
coldroom or chromatography refrigerator.

1

Open the shipping box and remove the foam packaging.

Carefully lift the FC from the box. Do not lift the FC by the
gantry; reach down and lift it from the base. Grip the base
of the FC by the sides to lift from the shipping box.

Check the box for the tubing kit, external power supply,

and USB cable. The tubing kit contains:



92 cm green polyetheretherketone (PEEK) tubing
from NGC union to dispense head for F100 systems
(fittings on both ends)



92 cm orange PEEK tubing for F10 systems (fittings
on both ends)



92 cm clear fluorinated ethylene propylene (FEP)
waste line from dispense head to union (fittings on
both ends)



150 cm clear FEP waste line from union to waste
bottle (fitting on one end)



Black magnetic union to be attached to side of NGC
System to act as anchor point for FC tubing; position
as necessary to avoid kinking any of the tubes

Dispense head height adjustment

Dispense head cover

Standard racks

2

Connect the power cable and USB to the back of the FC.

Plug in the power supply and connect the USB cable into
a USB port on the NGC Chromatography System.

USB por t Power port

Gantry

Drip tray

Plumbing the NGC Fraction Collector

Use the supplied tubing and union to plumb from the NGC System
to the dispense head on the fraction collector. Plumb the waste line
from the dispense head to the waste bottle via the union.

Rubbe r
grommet

Waste lin e

Sample tube

Cutout

Dispense head

1

Open the dispense head cover using a small

Dispense head
cover

Dispense head
waste por t

Phillips screwdriver.

3

Attach the waste line from the dispense head waste port to

the union on the side of the NGC System.

4

Route both tubes through the top of the dispense head

cover by sliding them into the rubber grommet. Finally, close
the dispense head cover and fasten with the screw.

2

Connect the sample tube to the dispense head. Use the

cutout in the back of the cover to insert a fittings tightener
and tighten.

5

Attach the tubes to the union so the direction of flow is as

shown below.

To FC

inlet port

waste por t

From FC

From NGC

To waste

bottle