Bio-Rad Gel Doc XR Instruction manual
MI170-8062BB Rev.G
Gel Doc XR
ChemiDoc XRS
170-8170 170-8070
170-8171 170-8071
Hardware Instruction Manual
Important
Please read these instructions before attempting to install or operate the Gel Doc XR or
ChemiDoc XRS.
Note: This instrument is suitable for research use only.
It must be used, therefore, only by specialized personnel that know the health risks associated with
UV radiation and with the reagents that are normally used with this instrument. Use of the acrylic
screen doesn’t guarantee protection of the user from UV radiation. The use of protective eyeglasses
or mask and gloves is strongly recommended.
Wichtig
Bitte lesen Sie die Anweisungen und Machen Sie sich mit der Bedienungsweise vertraut ,
bevor Sie den Hood benutzen.
Anmerkung: Dieses Geraet ist nur fuer Forschungszwecke geeignet
.
Ausserdem ist die Benutzung nur fuer spezialisiertes Personal gedacht, das mit den
Gesundheitsrisiken vertraut ist, die an die UV-Strahlung gebunden sind und den Reagentien, die
normalerweise mit diesem Geraet benutzt werden.
Die Benutzung eines Plexiglasschutzschildes garantiert dem Benutzer keinen Schutz vor UVStrahlung. Die Benutzung von Schutzbrille oder Schutzmaske ist strengstens empfohlen.
Warranty
The Gel Doc XR, and Chemi XRS are warranted against defects in materials and workmanship
for 1 year. If any defect occurs in the instrument during this warranty period, Bio-Rad Laboratories
will repair or replace the defective parts at its discretion without charge. The following defects,
however, are specifically excluded:
Defects caused by improper operation.
•
Repair or modification done by anyone other than Bio-Rad Laboratories or an authorized
•
agent.
Use of spare parts supplied by anyone other than Bio-Rad Laboratories.
•
Damage caused by accident or misuse.
•
Damage caused by disaster.
•
Corrosion caused by improper solvents or samples.
•
Garantie
Die Garantie fuer den Hood betraegt 1 Jahr auf Herstellungs- und Materialfehler.
Bei Auftreten von Fehlern waehrend der Garantiezeit repariert oder ersetzt Bio-Rad Laboratories
die fehlerhaften Teile auf eigene Kosten. Die folgenden Schaeden sind in jedem Falle
ausgeschlossen:
• Schaeden durch unsachgemaesse Bedienung bedingt.
Instandsetzungen oder Veraenderungen durch nicht authorisiertes Personal von
•
Bio-Rad Laboratories
• Benutzung von Ersatzteilen, die nicht von Bio-Rad Laboratories geliefert wurden.
• Schaeden durch Naturkatastrophen verursacht.
• Schaeden durch unsachgemaessen und fehlerhaften Gebrauch verursacht.
Korrosionsschaeden durch ungeeignete Loesungen oder Proben.
•
Page 2 of 42
IMPORTANT
Regulatory Notice
: The Gel Doc XR, and ChemiDoc XRS are designed and certified to meet
ENC1010, the internationally accepted electrical safety standards and EMC regulations and TUV.
Certified products are safe to use when operated in accordance with the instruction manual. This
instrument should not be modified or altered in any way. Modification or alteration of this
instrument will:
1. Void the manufacturer’s warranty.
2. Void the regulatory certifications.
3. Create a potential safety hazard.
NOTE
: Bio-Rad Laboratories is not responsible for any injury or damage caused by use of this
instrument for purposes other than those for which it is intended, or by modifications of the
instrument not performed by Bio-Rad Laboratories or an authorized agent.
Richtlinien
WICHTIG:
anerkannt von dem internationalen elektrischen Sicherheitsstandard EMC und unterliegt dessen
Richtlinien und TUV. Die Geraete sind sicher im Gebrauch, wenn die vorliegende Bedienungsanleitung beachtet wird. Dieses Geraet darf auf keinen Fall veraendert werden. Eventuelle
Veraenderungen fuehren zu:
1. Ungueltigkeit der Herstellergarantie
2. Ungueltigkeit des Zertifikats
3. Eventuelles Sicherheitsrisiko
Bemerkung:
hervorgerufen durch eine nicht vorhergesehene Benutzung des Geraetes. Ebenso uebernimmt BioRad Laboratories keine Haftung fuer Veraenderungen, die von nicht authorisierten Personen
durchgefuehrt wurden.
Dieses Geraet, von Bio-Rad Laboratories konstruiert, besitzt das Zertifikat ENC1010
Bio-Rad Laboratories haftet nicht fuer Verletzungen an Personen oder Sachschaeden,
Page 3 of 42
General Precautions
• Please read the instruction manual carefully.
The instrument must be used only for the intended purpose of gel documentation in research
•
laboratories.
• The instrument must be connected to a power source line which MUST BE grounded and
protected by a circuit breaker.
• Do not pour liquids directly on or inside the instrument.
Switch off all the lights immediately after use.
•
• Clean the transilluminator platen after use.
Generelle Vorsichtsmassnahmen
• Bitte die Bedienungsanleitung aufmerksam lesen.
• Das Geraet darf nur fuer die vorgesehenen Applikationen eingesetzt werden, d.h.
Geldokumentation in Forschungslaboratorien.
Fuer die Stromversorgung des Hood muss eine geerdtete Steckdose
•
benutzt werden.
• Keine Fluessigkeiten auf oder in das Geraet giessen.
• Die Lampe sofort nach dem Gebrauch ausschalten.
• Den UV-Filter des transilluminators nach Gebrauch reinigen.
Page 4 of 42
TABLE OF CONTENTS
HARDWARE INSTRUCTION MANUAL ..................................................................................................................1
SECTION 1 INTRODUCTION.....................................................................................................................................7
1.1 C
OMPUTER REQUIREMENTS
......................................................................................................................................7
1.2 PC ...........................................................................................................................................................................7
1.3 M
ACINTOSH
..............................................................................................................................................................7
SECTION 2 IMPORTANT SAFETY INFORMATION.............................................................................................8
2.1 I
MPORTANT NOTICE
2.2 P
OWER SAFETY INFORMATION
..................................................................................................................................................8
...................................................................................................................................8
SECTION 3 PRODUCT DESCRIPTION ....................................................................................................................9
3.1 CCD C
3.2 D
3.3 PCI
3.4 Q
3.5 P
3.6 P
AMERA AND LENSES
ARKROOM ENCLOSURE
INTERFACE CARDS
UANTITY ONE SOFTWARE
RINTER (OPTIONAL
ACKAGING
............................................................................................................................................................10
.......................................................................................................................................9
, E
MISSION FILTERS, AND ILLUMINATION SOURCES
...............................................................9
..........................................................................................................................................10
...................................................................................................................................10
) ..............................................................................................................................................10
SECTION 4 GETTING STARTED ............................................................................................................................12
4.1 S
ELECTING A LOCATION
4.2 A
SSEMBLING THE SYSTEM
..........................................................................................................................................12
........................................................................................................................................12
4.2.1 Assembling the Universal Hood II .................................................................................................................12
4.2.2 Assembling the CCD Camera ........................................................................................................................13
4.2.3 Connecting the cabling harnesses..................................................................................................................15
4.2.4 Installing the White Light Transilluminator (Optional)...............................................................................16
4.2.5 Installing the Optional 17 mm or 25 mm Wide-Angle Lenses (ChemiDoc XRS only):..............................16
4.3 Description of System Function and System Initialization................................................................................18
4.3.1 Control panel .................................................................................................................................................18
4.3.2 System Initialization .......................................................................................................................................21
4.3.3 Aligning the Camera assembly: .....................................................................................................................24
SECTION 5 OPERATION OF THE UNIVERSAL HOOD II.................................................................................25
5.1 O
PERATING THE UNIT
..............................................................................................................................................25
5.1.1 Switch on the Universal Hood II....................................................................................................................25
5.1.2 Position your gel ............................................................................................................................................25
5.1.3 Acquire an image ...........................................................................................................................................25
5.1.4 Acquiring an image with Flat Fielding:.........................................................................................................25
5.2 C
UTTING GELS
........................................................................................................................................................28
SECTION 6 TROUBLE SHOOTING.........................................................................................................................29
SECTION 7 ACCESSORIES AND REPLACEMENT PARTS...............................................................................30
Accessories and Replacement Parts........................................................................................................................30
SECTION 8 MAINTENANCE AND PART REPLACEMENT...............................................................................32
8.1 EPI-
8.2 F
8.3 UV T
ILLUMINATION LAMP REPLACEMENT
USE REPLACEMENT
RANSILLUMINATOR
................................................................................................................................................32
..........................................................................................................................................33
..................................................................................................................32
8.3.1Lamp replacement...........................................................................................................................................33
8.3.2 Starter replacement (P/N 100-1370)..............................................................................................................34
APPENDIX A: GEL DOC XR SYSTEM CABLE CONFIGURATION..............................................................35
APPENDIX B: CHEMIDOC XRS SYSTEM CABLE CONFIGURATION .......................................................36
APPENDIX C: INSTALLING SOFTWARE AND DRIVERS ................................................................................37
Gel Doc XR and ChemiDoc XRS- PC: .............................................................................................................37
Gel Doc XR and ChemiDoc XRS- Mac: ...........................................................................................................39
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ALSO READ THE RELEASE NOTES FILE FOR LAST MINUTE UPDATES FOR THE SOFTWARE. ......40
APPENDIX D: PCI CARD INSTALLATION...........................................................................................................41
Gel Doc XR – PC:................................................................................................................................................41
Gel Doc XR - Mac: ..............................................................................................................................................41
ChemiDoc XRS- PC:............................................................................................................................................41
Installing the PCI Digitizing Card ..........................................................................................................................41
APPENDIX E: TECHNICAL SPECIFICATIONS...................................................................................................42
Page 6 of 42
Section 1 Introduction
The Bio-Rad Gel Doc XR, and the ChemiDoc XRS Gel Documentation systems are easyto-use, high-performance systems. They use a CCD Camera to capture images in real time,
which allows you to more accurately position and focus the image. While using Bio-Rad
Quantity One software, acquired images can be optimized, annotated, analyzed, and printed.
The Bio-Rad Gel Doc XR, and the ChemiDoc XRS systems utilize an enclosure (Universal
Hood II) that is light tight and contains UV illumination and white light illumination, Please
note that both the Gel Doc XR and ChemiDoc XRS Cameras have external power supplies.
1.1 Computer Requirements
This software will run under Windows 2000 or XP, or on a Macintosh PowerPC.
The amount of computer memory required for using the program is mainly determined by the
size of the images you will scan and analyze. Images scanned at high resolution can be quite
large. For this reason, we recommend that you archive images on a network file server or
removable storage media.
1.2 PC
The following is the recommended system configuration for installing and running on a PC:
Operating system:
Processor:
RAM:
Intel Pentium 400 MHz or better
128 MB or more for Gel Doc XR, and ChemiDoc XRS systems.
Hard disk space:
Monitor:
17" monitor or better, 1024 x 768 resolution (absolutely required), True
Printer:
Optional.
1.3 Macintosh
The following is the recommended system configuration for installing and running on a
Macintosh:
Operating system:
Processor/Model:
RAM:
Hard disk space:
Monitor:
Printer:
256 MB or more for Gel Doc XR, and ChemiDoc XRS systems.
Optional.
Windows 2000, Windows XP,
3 GB or greater
color.
System Mac OS 10
PowerPC G3 processor or better.
3 GB
17" monitor, 1024 x 768 resolution (absolutely required), Millions of
colors.
Page 7 of 42
r
Section 2 Important Safety Information
2.1 Important Notice
Use of the Gel Doc XR, and ChemiDoc XRS involves UV illumination. Proper precautions
must be taken to avoid eye and skin exposure to the UV radiation. This instrument is meant for
use only by specialized personnel that know the health risks associated with UV radiation and
the reagents that are normally used with this instrument. The acrylic shield provides some UV
protection. However, it does not guarantee complete protection, and it is designed to shield
only the person working in front of the system.
Anmerkung: Dieses Geraet ist nur fuer Forschungszwecke geeignet
Ausserdem ist die Benutzung nur fuer spezialisiertes Personal gedacht, das mit den
Gesundheitsrisiken vertraut ist, die an die UV-Strahlung gebunden sind und den Reagentien,
die normalerweise mit diesem Geraet benutzt werden.
Die Benutzung eines Plexiglasschutzschildes garantiert dem Benutzer keinen Schutz vor UVStrahlung. Die Benutzung von Schutzbrille oder Schutzmaske ist strengstens empfohlen.
2.2 Power Safety information
a)
Voltage Setting Information:
chooses the correct voltage for your country or region.
b)
Fusing:
following location:
• F1 and F2 are located on the bottom rear panel and are a part of the power entry module,
The Universal Hood II has two user serviceable fuses. These are located at the
please see picture below for details:
WARNING: The operator should wear appropriate safety glasses or a
protective mask and gloves in addition to using the UV Safety Shield
provided with this instrument.
.
The Universal Hood II has a power supply that automatically
Fuse F1 and F2 are located in
this drawer. Use a screwdrive
and pull the drawer out to see
the fuses
Main power entry module
Page 8 of 42
Section 3 Product Description
3.1 CCD Camera and Lenses
A CCD Camera is placed on top of the Universal Hood II for capturing images. The Camera
comes with a Motorized Zoom Lens (MZL) that allows a remote adjustment of the lens control
functions viz. zoom, focus and iris.
For ChemiDoc
extreme light collection efficiency for low light applications like chemiluminescence.
Lens f 0.95, 25 mm, Wide Angle 1708072
Lens f 0.95, 17 mm, Wide Angle 1708073
3.2 Darkroom Enclosure, Emission Filters, and Illumination Sources
The Universal Hood II is designed to capture fluorescence and chemiluminescence images
without using a photographic darkroom. The enclosure has built-in white light epi-illumination
and a UV transillumination. An optional White Light Transilluminator (170-7950) or a White
Light Conversion Screen (170-8001) may also be used with the system.
For easy sample loading, the UV transilluminator is located in the drawer of the Universal
Hood II and can accessed from the front of the enclosure. For your convenience, the lights in
the darkroom enclosure turn off automatically after about 15 minutes. This time period can be
extended indefinitely by pressing the HOLD button.
The Universal Hood II has a three position Filter Slider that offers the flexibility of using two
different emission filters for fluorescence applications as well as the ability to image
chemiluminescence samples (no filter).
Bio-Rad Laboratories offers a selection of filters and illumination sources.
Standard Filter: Comes with each system.
548-630nm Amber Filter (Ethidium Bromide) 170-8081
Optional Filters: Can be purchased for Optimal performance.
520DF30 nm Optional Filter for SYBR Green/ SYBRGold/ GFP/
560DF50 nm Optional Filter for CY3/Rhodamine/Sypro Orange 170-8075
630BP30 nm Optional Filter for SYPRO Ruby/Texas Red 170-8076
480BP70 nm Optional Filter for Hoechst/Coumarin 170-8077
Optional Illumination Sources:
White Light Transilluminator 170-7950
White Light Conversion Screen 170-8001
302 nm UV lamps 6 ea. (shipped standard with each system) 170-8097
254 nm UV lamps 6 ea 170-8098
365 nm UV lamps 6 ea 170-6887
XRS systems optional lenses with low F-number are available that offer
Fluorescein 170-8074
Page 9 of 42
3.3 PCI interface Cards
Gel Doc XR:
The Gel Doc XR system requires a Fire Wire port to communicate with the computer where
the user interface software is installed. In case of computers that do not offer a Fire Wire
(IEEE1394) interface, a PCI Fire Wire card (included with the system) needs to be installed in
the computer to communicate with the Camera.
Note: Read and follow the instructions for installing the PCI card, drivers and Software
in Appendix C and D.
ChemiDoc XRS:
The ChemiDoc XRS system requires a PCI Digitizing card to communicate with the Camera.
This card needs to be installed in all PC and Mac systems with appropriate drivers (included in
the software).
Note: Read and follow the instructions for installing the PCI card, drivers and Software
in Appendix C and D.
3.4 Quantity One Software
Each system ships with 1 full version of Quantity One and unlimited copies of Quantity
One
Basic Mode. The software can be used to annotate and document images, analyze
molecular weights, print, and perform a host of other applications. See the software manual for
detailed instructions on how to install and operate the software.
Note: Read and follow the instructions in Appendix C and D prior to installing Quantity
One software.
3.5 Printer (Optional)
For your convenience, Bio-Rad offers an optional USB printer for use with the Gel Doc XR
and ChemiDoc
XRS:
Sony UPD895 (recommended for both the systems) 170-8066
3.6 Packaging
The Gel Doc XR, or ChemiDoc XRS systems consist of multiple components. Each of
them may be in a separate box or in one box with a label describing the catalog numbers and its
contents. Please make sure that all the system components are in your shipment. Please unpack
each component carefully and verify that you have the correct one. Each system includes the
following:
Page 10 of 42
Part # Descriptions
170-8170/170-8171 Gel Doc XR System PC/Mac
170-8062 Universal Hood II W/TLUM 100/240 Vac
170-8172 Gel Doc
170-8173 Gel Doc
170-8080 Kit Accessory, Universal Hood II
170-7964/7963 Cable, Serial, MZL, PC/Mac
170-9600 Quantity One
170-8009 Adapter, USB to Serial (Mac System only)
XR Camera and Cable w/MZL
XR Fire Wire card (PC System only)
Software PC/Mac
Part # Descriptions
170-8070/170-8071 ChemiDoc
170-8062 Universal Hood II W/TLUM 100/240 Vac
170-8088 ChemiDoc
PCI card and Camera Controller Cable)
170-8080 Kit Accessory, Universal Hood II
170-8008 Fluorescent Reference Plate
170-7964/7963 Cable, Serial, MZL, PC/Mac
170-9600 Quantity One
170-8009 Adapter, USB to Serial (Mac System only)
XRS System PC/Mac
XRS Camera W/MZL (including
Software PC/Mac
Page 11 of 42
Section 4 Getting Started
4.1 Selecting a Location
The Gel Doc XR and ChemiDoc XRS systems can be placed on any bench top. The best
position is near the computer where Quantity One
space to easily access the power switch on the Universal Hood II, which is located at the rear
bottom left side of the enclosure
4.2 Assembling the System
4.2.1 Assembling the Universal Hood II
Important: It is recommended that the Universal Hood II be carried by at
Be sure that the door and the drawer are completely closed.
Wichtig:
Geraeteboden halten.
a) Open the box and review the unpacking instructions.
b) Carefully remove all of the components of your system.
c) Remove the top foam and the plastic wrapping from the Universal Hood II.
d) Carefully pull the enclosure out of the bottom foam and place it in a suitable location.
Note: Please do not connect the power cable to a power source until all connections are
made. The power source has to be grounded and protected by a circuit breaker.
e) Locate the Amber Filter in the Accessory Kit and thread it onto one of the two 62 mm
rings of the Filter Slider (inside of the hood). The Filter Slider facilitates the selection
between two filters and an open position for chemiluminescence.
f) The three selectable positions are indicated by three symbols
II (filter 2)
Filter for future use. See pictures below.
least two people holding the instrument from the bottom side.
Es wird empfohlen , dass mindestens zwei Personen den
Hood transportieren und dabei das Geraet am
on the right side of the Camera support. Note the position of the Amber
will be operating. Remember to leave
O (no filter), I (filter 1) or
Three positions sliding filter
Page 12 of 42
4.2.2 Assembling the CCD Camera
Gel Doc XR
Bracket
Gel Doc XR
a) Place the Camera on the bench so that the two tripod mount holes are facing you.
b) Using one of the two knurled screws and washers attach the Gel Doc
bracket to the
Camera as shown in the picture below. Use the tripod mounting hole that is closest to the
top of the Camera. Do not tighten the screws yet.
c) The black adapter ring provided (3 millimeters thickness) has a soft and a hard side to it.
d) Position this adapter plate on top of the Universal Hood II such that the soft side of the
ring is facing up and the ring is concentric with the round opening on the universal hood.
Gel Doc XR bracket, washers, screws and adapter Gel Doc XR Camera and bracket
e) Place the Camera over the Universal Hood II by inserting the lens into the adapter ring.
f) Make sure that the front of the lens is in direct contact with the black adapter ring.
g) Verify that the lens is centered in the opening on top of the enclosure. To do this you may
need to move the entire body of the Camera and the ring so that the lens is also in the
center of the adapter ring.
WARNING: Failing to center the lens in the adapter ring and the hood can cause the
focusing ring located on the front side of the lens to mechanically rub or bind.
h) Secure the Gel Doc XR bracket (attached to the Camera) to the bracket of the Universal
Hood II using the screw and knurled knob (see pictures below)
i) Tighten the two screws of the Gel Doc
XR Camera bracket. Check that the lens is well
positioned on the black adapter ring for a complete light seal.
j) A completely assembled Camera will look like the picture shown below.
Screw, washer and knurled knob Gel Doc XR Camera assembled
Page 13 of 42
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