Bio-Rad GelAir Drying System User Manual

GelAir Drying
System
Instruction
Manual
Catalog Numbers
165-1771, 165-1772
For Technical Service Call Your Local Bio-Rad Office or in the U.S. Call 1-800-4BIORAD (1-800-424-6723)
Table of Contents
Figures and Tables.......................................................................................ii
Safety............................................................................................................iii
Section 1 Introduction
1.1 Overview ....................................................................................................................1
1.2 Unpacking...................................................................................................................1
Section 2 System Features............................................................................................2
2.1 GelAir Dryer...............................................................................................................2
2.2 GelAir Drying Frame.................................................................................................2
2.3 GelAir Assembly Table..............................................................................................3
Section 3 Set Up and Operation..................................................................................3
3.1 Drying Acrylamide Gels............................................................................................3
3.2 Drying Other Types of Gels.......................................................................................7
Section 4 Maintenance and Troubleshooting.............................................................9
Appendix A Specifications..............................................................................................10
Appendix B Warranty Information...............................................................................10
Appendix C Ordering Information................................................................................11
Appendix D References ...................................................................................................11
Figures and Tables
Figure 1 GelAir Drying System .................................................................................1
Figure 2 GelAir Dryer.................................................................................................2
Figure 3 Drying Frame...............................................................................................2
Figure 4 Drying Frame Assembly..............................................................................3
Figure 5 Adding the Top Layer of Cellophane.........................................................4
Figure 6 Removing Air Bubbles from Between the Sheets of Cellophane............4
Figure 7 Drying Frame Assembly..............................................................................5
Figure 8 Clamping the Drying Frame.......................................................................5
Figure 9 Trimming a Dried Gel .................................................................................6
Figure 10 Autoradiography Gel Assembly.................................................................7
Figure 1 1 Autoradiography Gel Assembly with Filter Paper Backing...................8
Figure 12 Agarose Gel Assembly.................................................................................9
Table 1 Approximate Drying Times for Polyacrylamide Gels in Heated
GelAir Dryer.................................................................................................6
ii
Safety
Caution/Warning
The GelAir Dryer is electrically isolated from earth ground to minimize the risk of elec-
trical shock to the user. These guidelines should be observed when using the gel dryer.
To insure adequate air flow, be sure that there is at least 10 cm of clearance around the gel dryer. Do not block the fan vent at the rear of the unit.
Always connect the power supply to a 3-prong, grounded AC outlet, using the 3 prong AC power cord provided with the dryer.
This instrument should not be modified or altered in any way. Alteration of this instrument
will void the manufacturer’s warranty, and create a potential safety hazard for the user.
Bio-Rad is not responsible for any injury or damage caused by the use of this instrument
for purposes other than for which it is intended or by modifications of the instrument not per­formed by Bio-Rad or an authorized agent.
Important
This instrument is intended for laboratory use only. This product conforms to the “Class A” standards for electromagnetic emissions intend-
ed for laboratory equipment applications. It is possible that emissions from this product may interfere with some sensitive appliances when placed nearby or in the same circuit as those appliances. The user should be aware of this potential and take appropriate measures to avoid interference.
This Bio-Rad product 115 V and 230 V is certified to meet CAN/CSA C22.2
No. 1010-1 and UL 3101-1 safety standards. Additionally the 230 V is certified to EN61010-1* safety standards. These products are safe to use when operated in accor­dance with the instruction manual.
This instrument should not be modified or altered in any way. Alteration of this instrument
will void the manufacturer’s warranty, void the CE, EN61010-1, CAN/CSA C22.2 No. 1010-1 and UL 3101-1 certification, and create a potential safety hazard for the user.
Bio-Rad is not responsible for any injury or damage caused by the use of this instrument
for purposes other than for which it is intended or by modifications of the instrument not per­formed by Bio-Rad or an authorized agent.
*EN61010-1 certification is an internationally accepted electrical safety and EMC standard for laboratory instrumentation, which includes the CE mark.
iii
Section 1 Introduction
1.1 Overview
Fig. 1. GelAir Drying System.
The GelAir Drying System can be used for drying practically any type of polyacrylamide
(SDS-PAGE, native, IEF, 2-D) or agarose gel. The GelAir Drying System produces dried gels in a format that is ideal for densitometry, autoradiography, or permanent storage. The GelAir system requires no vacuum pump or vapor trap, is practically maintenance free, and will dry up to 16 Mini-PROTEAN®II gels simultaneously and as quickly as a standard vac­uum dryer.
1.2 Unpacking
Upon receipt of your GelAir Drying System, carefully inspect the box for any damage
which may have occurred in shipping. Severe damage to the container may indicate damage to the system components. If any parts are missing or damaged, immediately file a claim with the carrier in accordance with their instructions and contact Bio-Rad Laboratories.
Unpack your Gel Drying System
The complete GelAir Drying System is shipped with the following:
GelAir Dryer with power cord Two Complete Drying Frames (molded bottom frame, stainless steel top frame and
clamps) Assembly Table Cellophane Support (50 pre-cut sheets) Instruction Manual
diagram 1
1
Dryer
Assembly Table
Clamps
Stainless Steel Top Frame
Molded Bottom Frame
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