Bio-Rad Chromatographic Surfaces User Manual

ProteinChip®NP20 Array
(Normal Phase)

Instruction Manual

Catalog #C57-30043

For technical support,
call your local Bio-Rad office, or
in the US, call 1-800-4BIORAD

(1-800-424-6723).

Uses

n

Binding of highly water-soluble proteins

n

On-chip sample cleanup

n

Calibration of the ProteinChip SELDI system instrument using
peptides or proteins of known molecular weight

How It Works

Silicon oxide groups on the active spots of the ProteinChip NP20
array allow proteins to bind through hydrophilic and charged
residues, including serine, threonine, and lysine. This surface is
commonly used for microscale sample cleanup. Washing with
water removes buffer components such as salts and detergents.

Packaging and Storage

Store the arrays at room temperature.

ProteinChip arrays are packaged in a 12-array cassette.
A bioprocessor reservoir is included in the package (see Figure 1).
The spare ProteinChip cassette included to separate the reservoir
from the arrays should be removed before use in the ProteinChip
cassette-compatible bioprocessor (catalog #C50-30011). It is not
necessary to remove the arrays when using the cassette-compatible
bioprocessor; however, individual arrays can be removed if needed.
To do this, remove the bioprocessor reservoir before taking any
arrays out of the cassette. Be careful not to touch the spots on the
array. A pair of ProteinChip array forceps (catalog #C20-10002)
helps effectively remove the arrays from the cassette (see Figure 2).

Fig. 1. ProteinChip cassette and
reservoir.

© 2006 Bio-Rad Laboratories, Inc.

Fig. 2. Removal of ProteinChip arrays
from cassette using array forceps.