Bio-Rad Chelex 100 Molecular Biology Grade Resin User Manual

InstaGeneTMMatrix

Catalog #
732-6030

Table of Contents

Section 1. Introduction........................................................1

1.1 Contents and Storage.................................................................1

1.2 Warning ......................................................................................1

1.3 Use .............................................................................................1

Section 2. Instruction for Use .............................................2

2.1 DNA Preparation From Whole Blood...........................................2

2.2 DNA Preparation From Cultured Mammalian Cells ......................3

2.3 DNA Preparation From Bacteria .................................................4

Section 1. Introduction

1.1 Contents and Storage

This bottle contains 20 ml of 6% InstaGene matrix and a magnetic
stirbar. This is sufficient for 100 DNA preparations. Upon arrival, store
the matrix at 4 °C.

1.2 Warning

Avoid prolonged exposure of the matrix to UV light.

1.3 Use

InstaGene matrix allows fast and easy preparation of PCR*
amplifiable DNA by eliminating labor intensive phenol/chloroform
extraction steps. A simple cell lysis step by boiling in the presence of
the matrix is sufficient. This is possible because the matrix efficiently
absorbs cell lysis products that interfere with the PCR amplification
process. Procedures for generating DNA suitable for PCR from whole
blood, cultured cells, and bacteria using InstaGene matrix are
described on the following pages. Protocols for other types of tissues
or cells are presently being investigated.

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