Bio-Rad Bio-Scale MT High-Resolution Empty Columns User Manual

Bio-Scale
Columns

Instruction Manual

Catalog numbers
751-0081
751-0083
751-0085
751-0087

™

MT

Table of Contents

Section 1 Characteristics of Bio-Scale MT2,

MT5, MT10, and MT20 Columns ......................... 1

1.1 Introduction ........................................................... 1

1.2 Column Description ............................................... 1

1.3 Column Specifications ........................................... 2

Section 2 Use of the Bio-Scale MT Columns ..................... 2

2.1 Column Disassembly ............................................. 4

2.2 Column Assembly .................................................. 4

2.3 Column Packing .................................................... 5

2.4 Connections to Chromatography Systems ............. 7

Section 3 Care of the Bio-Scale MT Colums ...................... 8

3.1 Frit Removal .......................................................... 8

3.2 Column Bed Top-Off ............................................. 8

Section 4 Solvent Compatibility Table ................................ 9

Section 5 Product Informations ......................................... 10

Section 1
Characteristics of the Bio-Scale MT2, MT5, MT10,
and MT20 Columns

1.1 Introduction

The Bio-Scale MT columns have been designed specifically
to provide high resolution chromatography of bio-molecules
when packed with any suitable chromatography media. Typical
packings might include ion exchange, hydrophobic interaction,

hydroxyapatite, or affinity chromatography media.
The Bio-Scale MT range consists of four sizes (2, 5, 10, and

20 ml), allowing easy scale-up of separation and purification
protocols. The column design has been optimized to provide
easy packing, equilibration, bed-height adjustment, and sample
application. All column parts are bio-compatible for preservation of
sample biological activity.

The Bio-Scale MT column range is designed for use with
Bio-Rad’s BioLogic System, as the column fittings are 1/4-28
Super-flangeless. Adaptors and unions are available from Bio-Rad
for connection of these columns to other medium or high pressure

systems.

1.2 Column Description

Each column consists of a borosilicate glass tube held in place by
two endcaps and a plastic safety shield. The lower bed support
consists of a fixed-position frit and a distribution screen assembly.

Bed height adjustment is provided by the upper bed support,
which is positioned and held in place using a threaded lock-nut.
A removable frit protects the top of the bed and supports a thin
distribution screen which insures efficient sample application to the
bed itself.

Each column can be packed with chromatography media using
either dry or slurry packing methods. A minimum particle size of

5 microns is recommended.

Bio-Scale MT Column Instruction Manual 1