Bio-Rad Bio-Scale Mini Macro-Prep High Q and Macro-Prep High S Cartridges User Manual
Bio-Scale™Mini
Macro-Prep®High Q,
High S and DEAE
Cartridges, 1 and 5 ml
Instruction Manual
Catalog #
732-4120, 732-4122, 732-4124,
732-4130, 732-4132, 732-4134,
732-4140, 732-4142, 732-4144
Table of Contents
Section 1...Introduction ........................................1
Section 2 Connecting to Bio-Rad’s
Low-Pressure Chromatography
Instruments ........................................5
Section 3 Connection to Other Liquid
Chromatography Systems................10
3.1 BioLogic DuoFlow
™
Systems.................................10
3.2 HPLC Systems ......................11
3.3 FPLC Systems .......................12
Section 4 Preparing a Cartridge for Use...........13
4.1 Sample Preparation ...............14
4.2 General Purification
Protocol .................................15
4.3 Scaling Up the Separation .....16
Section 5...Care of the Cartridge........................18
5.1 Regeneration .........................18
5.2 Cleaning.................................18
5.3 Storage..................................20
Section 6 Technical Assistance ........................20
Section 7 Ordering Information.........................21
Section 8 References .......................................23
Section 1
Introduction
Bio-Scale Mini cartridges have a patent-pending
double-wall design that provides extra durability and
allows easy, reliable runs with aqueous buffers
commonly used for protein purification. The
polypropylene luer fittings and internal sealing
surfaces ensure leak-free operation at pressures up
to 45 psi. Bio-Scale Mini cartridges are convenient,
disposable, and supplied ready for use. They are
easy to use and prepacked for fast, reproducible
chromatographic separations. Cartridges are available for a variety of chromatographic techniques,
including desalting, ion exchange, affinity, and
mixed-mode chromatography. The design of
Bio-Scale Mini cartridges offers:
• Ready-to-go convenience; simply equilibrate
the cartridge in the buffer of choice
• Luer fittings for convenient connection to any
chromatography system or directly to a
Luer-Lok syringe
1
Bio-Scale Mini Macro-Prep High Q and S and
Macro-Prep DEAE cartridges are packed with
Macro-Prep
®
ion exchange media. These media are
based on hydrophilic spherical polymeric beads
designed for the purification of proteins, nucleic
acids, viruses, plasmids, and other macromolecules.
Macro-Prep beads are designed to provide medium
capacity, low backpressure, and high productivity.
Detailed product information is given in Tables 1
and 2. See Ordering Information for a listing of the
complete Bio-Scale Mini cartridge product line.
2
Table 1. Bio-Scale Mini Macro-Prep High Q,
High S, and DEAE cartridge specifications.
Sizes 1 ml and 5 ml bed volumes
Dimensions
1 ml: 40 mm length x 5.6 mm inner diameter
5 ml: 40 mm length x 12.6 mm inner diameter
Maximum pressure tolerance 45 psi
Operational flow rates 1 ml: 1–6 ml/min (240–1460 cm/hr)
5 ml: 1–15 ml/min (50–720 cm/hr)
Fittings: Female luer inlet;
male luer outlet
Column material Polypropylene
Frit material Polyethylene (HDPE)
Shipping conditions 20% ethanol
Storage recommendations 20% ethanol
Autoclavability Not autoclavable
3
Table 2. Macro-Prep High Q & S and
Macro-Prep DEAE specifications.
Properties High Q High S DEAE
Type of ion exchanger Strong anion Strong cation Weak anion
Functional group -N+(CH3)
3
-SO
3
-
-N+(C2H5)
2
Total ionic capacity 400 ± 75 µeq/ml 160 ± 40 µeq/ml 175 ± 75 µeq/ml
Dynamic binding capacity* ≥37 mg BSA/ml ≥49 mg IgG/ml ≥30 mg BSA/ml
Shipping counterion Cl
-
Na
+
Cl
-
Chemical stability
1% SDS, 24 hr Yes Yes Yes
6M guanidine-HCl, 24 hr Yes Yes Yes
pH stability 1–10 1–12 1–10
Total organic carbon in ppm, after 24 hours at 22°C
0.1 M HCl 0 4 0
0.2 M H
3PO4
0130
0.01 M NaOH 28 N/A N/A
0.1 M NaOH 200 31 26
1.0 M NaOH 337 49 121
Antimicrobial agent 20% ethanol 20% ethanol 20% ethanol
Regeneration 1–2 M NaCl 1–2 M NaCl 1–2 M NaCl
Storage conditions 20% ethanol 20% ethanol 20% ethanol
* 10% breakthrough capacity determined in a 1.1 x 20 cm column.
4
Section 2
Connecting to Bio-Rad’s
Low-Pressure
Chromatography
Instruments
Bio-Scale Mini cartridges are ideal for use with
Bio-Rad's BioLogic
™
LP chromatography system,
Econo
™
gradient pump, the patented* Model EP-1
Econo
™
pump, and all low-pressure chromatography instruments. Bio-Scale Mini cartridges can be
conveniently connected directly to the system using
the luer fittings on the cartridge.
1. Install 1.6 mm inner diameter (ID) tubing in the
pumphead. Adjust the platen pressure screw
(on pumphead) – using a screwdriver or coin,
turn the screw counterclockwise as far as it will
go, then turn clockwise three full turns.
* US patent 5,135,658
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