BioLogic DuoFlow
™
Chromatography System
5.0 Software
New Features
For technical service
call your local Bio-Rad office or
in the U.S. call 1-800-4BIORAD
(1-800-424-6723)
On the Web at http://www.discover.bio-rad.com
The BioLogic DuoFlow version 5.0 software introduces three new features and several
enhancements to BioLogic DuoFlow
™
version 4.0 while maintaining the familiar intuitive
and easy-to-use design.
New Features
• Scouting Wizard
• Buffer Editor
• Method Templates
Enhanced Features
• Below Threshold Fraction Collection
• Rack & Tube # and Rack & Grid # BioFrac
™
fraction collector numbering
schemes
• Time and Volume based chromatograms
• New Buffer Blending buffer systems
• Improved High Flow non Buffer Blending feature
• Vertical chromatogram trace shifting in Trace Compare overlay view
• Trace Compare display settings saved
• Enhanced Online Help
New Features
Scouting Wizard
The Scouting Wizard is a feature that automates the setup of scouting experiments.
Scouting is a procedure used to systematically optimize the purification conditions for a
specific target molecule(s) (i.e. protein). Molecules such as proteins differ from one
another in their charge, hydrophobicity, solubility, reactivity, substrate specificity and
intermolecular interactions. A purification protocol that is satisfactory for one molecule
may not work for another molecule. Several factors influence the quality of a purification
procedure. Some of these factors include: buffer composition (pH, ionic strength,
co-solutes), elution type (gradient slope and gradient duration), flow rate, column chemistry
and sample composition. In principle, each of these can be adjusted to produce the most
efficient and effective purification procedure for a molecule. However, in practice, only a
few of these are generally tested due to time and cost considerations. By performing
Scouting as a set of automated runs, the time and resources required for protocol
optimization can be significantly reduced.
Buff
er Editor
The Buffer Editor is used to create new Buffer Blending buffer systems and to modify existing
buffer systems. Buffer Blending is a feature of the BioLogic DuoFlow Maximizer
™
and
BioLogic DuoFlow Pathfinder
™
systems that dynamically "blends" the conjugate acid and
base of a buffer with water and salt to produce a solution with a specific salt concentration
and pH. The Buffer Editor can be used to create new Buffer Blending buffer systems from
user-supplied information. Each buffer system can include up to three buffers and each buffer
can have up to three pKa's. The Buffer Editor also generates recipe text that describes how
to make each Buffer Blending solution. The Buffer Editor requires information about the buffer
( pKa(s), temperature coefficient(s), charge state, molecular weight and concentration) and
the salt (charge state molecular weight and concentration). This information can also be
supplemented with user determined 1 or 2 point pH corrections (entered in the Buffer
Blending setup dialog). For multiple component buffers, pH corrections can be included for
each buffer in the buffer system, to improve the pH accuracy over the entire buffer pH range.
The Buffer Editor can also be used to copy and modify any of the 26 predefined Buffer
Blending buffer systems. Each of these buffer systems can be modified to accommodate
reagents that are readily available in your laboratory.
Method Templates
The BioLogic DuoFlow software provides a variety of method templates to simplify the
method creation process. Templates for a variety of chromatography techniques such as
affinity, chromatofocusing, gel filtration, hydrophobic interaction, hydroxyapatite and ion
exchange can be loaded from the New Methods dialog box. Each template includes a
brief method description with its application and the devices required to run it. Pressing
"View Template Help" easily accesses a more detailed description. These templates can
be used as is, or modified to fit experimental requirements. The templates that are available
depend on the system configuration (BioLogic DuoFlow
™
, BioLogic DuoFlow QuadTec™,
BioLogic DuoFlow Maximizer and BioLogic DuoFlow Pathfinder systems).
In addition to the Templates available from the New Method dialog, multi-dimensional
chromatography templates have been included in a file named "Multi-D Chromatography
Templates.ZIB" in the BioLogic directory on your hard disk. These templates are saved in
the file according to the type of system they are designed for (BioLogic DuoFlow,
BioLogic DuoFlow QuadTec, BioLogic DuoFlow Maximizer or BioLogic DuoFlow
Pathfinder systems). Additional information is available in the BioLogic
™
software online
help Contents.
Enhanced Features
Below Threshold Fraction Collection
Threshold collection can now be set to occur below a defined threshold. This feature is
especially valuable for desalting and chromatofocusing applications where it may be
important to collect fractions below a set conductivity or pH reading.
Rac
k & Tube # and Rack & Grid # BioFrac fraction collector tube numbering
This software enhancement allows users to more easily identify fractions collected into
grid numbered racks, such as microtiter plates. In "Rack & Tube #" mode, the racks and
tubes are numbered in the order that they are filled. In "Rack & Grid #" mode the racks
are numbered in the order that they are filled and the tubes are numbered by the grid
position in the rack where the tube is located. Switching between the two modes is done
from the View menu and can be done at any time.
Time and
Volume based chromatograms
This feature allows users to view chromatograms in both time mode and volume mode.
Users may switch between time and volume mode at any time. Volume mode displays
the run chromatogram in units of ml and time mode displays units in "minute.tenth" or
"HH:MM:SS". The default time unit is set in Options/Edit User Preferences. In Trace
Compare, volume mode is useful when comparing runs collected at different flow rates.
Ne
w Buffer Blending buffer systems
Two new predefined Buffer Blending buffer systems have been included in the BioLogic
DuoFlow 5.0 software. "N-methylpiperazine/Bis-Tris/Tris" is a broad range (pH 4.7 to 9.4)
multi-component buffer for applications that require a broad buffer pH range, such as pH
scouting. "Phosphate with Ammonium Sulfate" is a single component buffer for
hydrophobic interaction chromatography that uses 2.4 M ammonium sulfate as the salt.
"High Flo
w Non-Buffer Blending (1x)" Buffer System
This buffer system is used to run buffers of fixed composition (non-blending) at the high
flow rates available to Buffer Blending applications. When used, this buffer system allows
flow rates up to 20 ml/min (F10 pump heads) and 80 ml/min (F40 pump heads). For this
buffer system, buffers A and B are prepared at a 1x concentration rather than the 2x
concentration required for Buffer Blending. Buffer A is plumbed into the BioLogic
Maximizer A1 and B1 inlets, and Buffer B is plumbed into A2 and B2 inlets.
T
race Shifting in the Overlay View of Trace Compare
Shift Up and Shift Down functions have been added to the Trace Compare toolbar that
are used to shift traces vertically with respect to each other. Shifting allows the features of
individual chromatograms to be observed when comparing multiple chromatograms.
T
race Compare settings Retained
Trace Compare now retains display settings for each Compare. This facilitates the
retrieval of saved "views" of the data.
Online Help
The BioLogic 5.0 software online help has several enhancements including more than 60
new help topics and a Contents view.
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