Bio-Rad Bio-Gel A Agarose Gel User Manual

Bio-Gel®A Gels

Instruction Manual

Table of Contents

Page

Section 1 Introduction ..............................................1

Section 2 Technical Description................................2

Section 3 Principles of Gel Filtration ......................4

3.1 Gel Filtration Basics and Sample

Preparation ..................................................4

3.2 Column Selection ........................................6

3.3 Eluant Selection ..........................................6

Section 4 Column Packing and Operation ..............7

4.1 Column Packing ..........................................7

4.2 Column Operation ......................................9

4.3 Void Volume Determination

and Calibration ............................................9

Section 5 Storage and Sterilization ..........................11

Section 6 Product Information..................................12

Section 1
Introduction

Bio-Gel A gels are a series of agarose based size
exclusion gels which provide high resolving power,
minimal non-specific interaction, and excellent flow
properties. The porosity of the gel is controlled by the
percentage of agarose incorporated into the matrix. Six
fractionation ranges are available, with exclusion limits of

0.5, 1.5, 5, 15, and 50 million daltons.

Bio-Gel A gels are compatible with all commonly
used aqueous buffers. The gel has excellent shrink/swell
properties and exhibits no significant change in the bed
volume with increasing salt concentration up to 500 mM
salt. The gels can be run between pH 4–13 and can be
used at temperatures between 2–30 °C.

1