BioFrac Fraction Collector
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BioFrac™ Fraction Collector
User Guide
Version 2.0
BioFrac™ Fraction Collector
User Guide
Version 2.0
Catalog # 741-0002
Bio-Rad Technical Support Department
The Bio-Rad Technical Support department in the U.S. is open Monday through Friday,
5:00 AM to 5:00 PM, Pacific Standard Time. Worldwide technical support is available on
the Web at www.consult.bio-rad.com.
Phone: 1-800-424-6723, option 2
Fax: 1-510-741-5802
Email: LSG.TechServ.US@Bio-Rad.com (U.S.)
LSG.TechServ.Intl@Bio-Rad.com (International)
Web: www.consult.bio-rad.com
Notice
No part of this publication may be reproduced or transmitted in any form or by any
means, electronic or mechanical, including photocopy, recording, or any information
storage or retrieval system, without permission in writing from Bio-Rad.
Bio-Rad reserves the right to modify its products and services at any time. This user guide
is subject to change without notice. Although prepared to ensure accuracy, Bio-Rad
assumes no liability for errors or omissions, or for any damage resulting from the
application or use of this information.
Combicon is a trademark of Phoenix Contact, Inc. Costar is a trademark of Corning, Inc.
Dell is a trademark of Dell Computer Corp. Eppendorf is a trademark of Eppendorf AG.
PEEK is a trademark of Victrex PLC. Scienceware is a trademark of Bel-Art Products.
Tefzel is a trademark of E.I. du Pont de Nemours & Co. Titertube is a trademark of Nortech
Laboratories Inc. Tygon is a trademark of Norton Co. Windows is a trademark of Microsoft
Corporation.
Copyright © 2001–2012 by Bio-Rad Laboratories. All rights reserved.
Table of Contents
Chapter 1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
Unpacking . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
Physical Description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Chapter 2 Front and Rear Panel Controls and Connectors . . . . . . . 9
Front Panel Controls . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
Rear Panel Connectors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Chapter 3 System Configuration and Plumbing . . . . . . . . . . . . . . . 13
Fraction Collector Setup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
Connecting Instruments and Devices to the BioFrac Fraction Collector . . . . . 16
Connecting to a BioLogic DuoFlow Chromatography System . . . . . . . . . . . 17
Connecting to a BioLogic LP System or to a Model EP-1 Econo Pump . . . 18
Connecting to the Econo Gradient Pump and a Chart Recorder . . . . . . . . . 20
Connecting the Model EP-1 Econo and Econo Gradient Pumps. . . . . . . . . 21
Connecting to Components Not Manufactured by Bio-Rad . . . . . . . . . . . . 21
Setting Up the Fraction Collector Racks . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
Chapter 4 The User Interface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
Main Screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
Main Screen (Local Mode) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
Main Screen (LP/Econo Mode) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
Run Screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
The Run Screen in Local Mode. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
User Guide | iii
Table of Contents
The Run Screen in LP/Econo Mode . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
Rack Screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46
Method Library Screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48
Calibration Screen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49
Advanced Collection Screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
Collection Windows Table Screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54
Results Screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56
Chapter 5 Stand-Alone Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
Collect All . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
Peak Detection by Threshold . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61
Collect by Windows. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
Collect by Windows and Threshold. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
Collect Using a Delay Function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
Example: Collect All with Delay . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70
Example: Peak Collection by Threshold and Delay. . . . . . . . . . . . . . . . . . . . 71
Example: Collection Using Time Windows and Delay. . . . . . . . . . . . . . . . . . 73
Chapter 6 LP/Econo Mode Operation. . . . . . . . . . . . . . . . . . . . . . . . 75
Chapter 7 Maintenance and Troubleshooting . . . . . . . . . . . . . . . . . 79
Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79
Troubleshooting. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81
Appendix A Bubble Filter Time . . . . . . . . . . . . . . . . . . . . . . . . . . . 83
Appendix B Rear Panel Connector Information. . . . . . . . . . . . . . 85
15-Pin D Connector. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 86
8-Pin Mini-DIN Connector . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88
3-Pin Combicon Connector. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89
iv | BioFrac Fraction Collector
Table of Contents
Appendix C Specifications. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91
Appendix D Warranty and Ordering Information . . . . . . . . . . . . . 93
Warranty Information. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 94
Ordering Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 94
User Guide | v
Table of Contents
vi | BioFrac Fraction Collector
1 Introduction
Overview
The BioFrac™ fraction collector provides automated collection options for analytical
and preparative chromatography applications. It can be used as a stand-alone
collector or as a companion to any chromatography system. The BioFrac fraction
collector is capable of performing basic to complex fraction collection schemes and
can be used at flow rates up to 100 ml/min. The fraction collector accommodates
numerous rack options from microplates to bottles and carboys.
User Guide | 1
1 | Introduction
Fig. 1. BioFrac fraction collector with two F1 racks (12–13 mm tubes).
Features
The key features of the BioFrac fraction collector include the following:
Microprocessor control with easy-to-use front panel controls and a
menu-driven software interface for method setup
Method library for saving up to 20 user-defined fraction collection methods
Local and remote starting of the fraction collector
Collection by time, volume, or drops
2 | BioFrac Fraction Collector
Overview
Advanced fraction collection functions. Peak detection, Time, or Volume
windows (up to 20) or a combination of Peak Detection with Time or
Volume windows
Manually adjustable control module that accommodates tube heights up to
150 mm
Compatibility with chromatography systems from Bio-Rad as well as other
manufacturers
Modular system that can be stacked directly on top of the NGC™,
BioLogic DuoFlow™, and BioLogic™ LP chromatography systems
Accommodates several inexpensive, off-the-shelf racks for tubes
(12–20 mm and 30 mm diameter), Eppendorf tubes or microcentrifuge
tubes (0.5 ml, 1.5 ml, and 2 ml) and scintillation vials. Racks are
autoclavable
Ability to collect up to 180 fractions in tubes or 384 fractions in 96-well
microplates
Optional Prep-20 adaptor for preparative collection in up to 20 collection
vessels of any size, from milliliter to liter collection volumes
Optional ice bath (with tube grips) that doubles as a holder for microplates.
Tube grips hold tubes firmly in any position while decanting. Rack allows
cooling of 13 mm tubes on ice. As a microplate holder it accommodates
12-, 24-, 48-, and 96-well microplates and Titertube tubes that adhere to
SBS standards for microplates
Diverter valve to divert flow and minimize spillage during tube changes.
Diverts unwanted eluent to waste and eliminates spills during fraction
advances
Multirun capability for overlaying fractions or collection of experiments
sequentially
Serpentine arm movement. Can be changed to a column or row pattern for
microplates and Titertube tubes
User Guide | 3
1 | Introduction
Optional drop former optimized for small-volume drop dispensing
Ability to start/stop an external pump and chart recorder
Screen sleep mode for longer display life
Unpacking
When unpacking the fraction collector, carefully inspect the containers for any
damage that may have occurred in shipping. Severe damage to a container may
indicate damage to its contents. If you suspect damage to the contents may have
occurred, file a claim immediately with the carrier in accordance with their
instructions before contacting Bio-Rad Laboratories.
Caution: Do not lift the fraction collector by its dispenser arm.
Grip the base of the fraction collector and lift it slowly out of its packing. Do not lift
the unit by its dispenser arm. Remove the remaining contents from each of the
boxes and check all of the parts against the supplied packing list. The BioFrac
fraction collector is shipped with the following:
Fraction collector unit
(recommended when collecting into microplates)
AC power cord
Fittings kit (Figure 2)
Instruction guide
Tube rack #F1 (2) for 12–13 mm tubes
Diverter valve (including an hex key)
Combicon connector (included with instruction guide)
Note: If any part is missing or damaged, contact Bio-Rad Laboratories
immediately.
4 | BioFrac Fraction Collector
Fig. 2. Fittings kit.
Overview
LEGEND
Item Quantity Contents of Fittings Kit
1 1 1.0 m Tefzel tubing, 1/16" OD, 0.030" ID, with one 1/4-28
fitting
2 4 Fittings, 1/4-28, 1/16" OD
3 4 Ferrules, 1/16" OD
4 2 Unions, luer to 1/4-28
5 1 26” PEEK tubing, 1/16" OD, 0.030" ID, with two 1/4-28
fittings labeled collect HF
6 1 26” PEEK tubing, 1/16" OD, 0.020" ID, with two 1/4-28
fittings labeled collect
User Guide | 5
1 | Introduction
Physical Description
Figure 3 identifies the physical features of the BioFrac fraction collector, and the
accompanying table describes the features in greater detail.
Fig. 3. Physical features of the BioFrac fraction collector.
6 | BioFrac Fraction Collector
Overview
Feature Description
Control module The fraction collector head contains the
display, function keys, and alphanumeric
keypad (see
Controls and Connectors
height may be adjusted to accommodate tube
heights up to 150 mm.
Base The fraction collector base holds the rack
positioning tray, power switch, and I/O
connectors. It is designed so that it can be
stacked on top of the NGC, BioLogic DuoFlow,
and BioLogic LP chromatography system
workstations if benchspace is limited.
Columns The columns hold the fraction collector head
and serve as a rack for mounting the diverter
valve or other devices, such as a pH probe and
a UV monitor, using Bio-Rad bar clamps
(Cat. #750-0265).
Rack positioning tray Accurate dispensing requires that racks be
positioned using the rack-positioning tray.
Molded indents on the tray allow the rack feet
to be placed securely on the rack-positioning
tray. The tray holds two full racks (F1, F2, or F3)
on one face and can be inverted for positioning
of four half-racks (H1, H2, H3, or H4). See
Chapter 2, Front and Rear Panel
, on page 9). Its
Chapter 3, System Configuration and
Plumbing
options.
Dispenser arm The dispenser arm moves the drophead in a
serpentine motion over each of the collection
tubes. The arm movement can be changed to a
column or row pattern for microplates and
Titertube tubes.
, on page 13 for available rack
User Guide | 7
1 | Introduction
Feature Description
Drophead Consists of the drop former, a photodiode cell
Diverter valve The diverter valve minimizes spillage during
Lock pin The height adjustment lock pins, located on
for drop counting, and a clear glass tube that
protects the photodiode cell from splashes. The
inlet tubing is connected to the drop former,
which provides uniform drop size. The drop
former accepts 1/4-28 fittings. An optional drop
former is available that has been optimized for
small-volume drop dispensing (25 µl drops).
drophead movement. The BioFrac diverter
valve is designed to have a minimal internal
volume and is 100% flushed in order to
minimize loss of chromatographic resolution.
either side of the front of the control module,
are used to secure the drophead height.
8 | BioFrac Fraction Collector
2 Front and Rear Panel
Controls and Connectors
The controls and connectors on the front and rear of the panel are illustrated and
described in this chapter.
Front Panel Controls
Fig. 4. Front panel controls.
User Guide | 9
2 | Front and Rear Panel Controls and Connectors
Table 1. Front panel controls
Feature Description
Cursor keys Used to move the cursor on the LCD display up, down,
left, or right.
Function keys These five keys are located directly below the LCD
display. The function each key executes is displayed
above it.
Clear Entry key Clears a cursor field or closes a menu option list.
Decimal point key Used to enter a decimal point.
Enter key Accepts a numeric value that has been entered.
Alphanumeric keypad Used to enter a decimal value in numeric fields and
alphanumeric characters in text fields. In a text field, the
character displayed is incremented with each press of
the keypad (for example, pressing the 2 key in turn
displays 2, A, B, C, 2, and so on.)
Rear Panel Connectors
Fig. 5. Rear panel connectors.
10 | BioFrac Fraction Collector
Table 2. Rear panel connectors
Connector Description
Display cable – connects the fraction collector control
module to the power supply and other ports in the base of
the fraction collector.
Diverter valve connector – used to connect the diverter
valve to the fraction collector.
I/O connector – a 15-pin D connector used for
connecting the following instruments to the BioFrac
fraction collector:
BioLogic™ LP chromatography system,
Model EP-1 Econo
fraction advances.
Econo gradient pump. The fraction collector controls all
fraction collection parameters.
External pump. With a BioFrac accessory cable (15-pin
to bare wires) connected to the I/O port, the fraction
collector can receive start, stop, and fraction advance
signals from an external pump. (The pump must have
compatible control circuitry logic. See
Rear Panel Connector Information
Instrument Bus – for connecting the BioFrac to a
BioLogic DuoFlow™ system using system cables 17, 18,
19, or 21.
Rear Panel Connectors
™
™ pump. This system controls
Appendix B,
, on page 85.)
User Guide | 1 1
2 | Front and Rear Panel Controls and Connectors
Table 2. Rear panel connectors, continued.
Connector Description
Rec connector – this 8-pin mini-DIN connector controls
the diverter valve or a chart recorder:
When connected to a BioLogic LP system or
Model EP-1 Econo pump, the connector receives
diverter valve control signals.
When connected to a chart recorder, the fraction
collector controls paper feed, pen up/down, and event
marks. (The chart recorder must have compatible
control circuitry logic. See
Connector Information
Combicon connector – used to connect a UV monitor or
other detector for Peak Detection by Threshold. (See
Chapter 3, System Configuration and Plumbing, on
page 13 for cable information.)
Appendix B, Rear Panel
, on page 85.)
12 | BioFrac Fraction Collector
3 System Configuration and
Plumbing
The BioFrac™ fraction collector is shipped assembled and requires minimal
plumbing and cabling to prepare it for use. Once assembled, the BioFrac device can
be operated in the following configurations:
BioLogic DuoFlow system control
LP/Econo™ mode collection with Bio-Rad components
Stand-alone fraction collection (Local mode) with Bio-Rad components
Stand-alone fraction collection with components that are not
manufactured by Bio-Rad
The following sections of this chapter describe how to set up the BioFrac fraction
collector and how to connect instruments and devices to work with it.
User Guide | 1 3
3 | System Configuration and Plumbing
Fraction Collector Setup
Fig. 6. Plumbing the BioFrac fraction collector.
14 | BioFrac Fraction Collector
Fraction Collector Setup
To set up the BioFrac fraction collector
1. Place the fraction collector on a level surface on a laboratory or coldroom
bench or in a cold cabinet. Alternatively, if benchspace is limited, the modular
fraction collector can be stacked on top of the NGC, BioLogic DuoFlow™, or
BioLogic™ LP chromatography system workstations.
2. Connect the fraction collector display cable to the port labeled Display on the
back of the fraction collector. (See Figure 5 on page 10.)
3. Connect the diverter valve cable to the Diverter Valve port on the back of the
fraction collector. (See Figure 5 on page 10.)
4. Attach the diverter valve to either the right or left column. The diverter valve
should be attached so that the Collect port is pointed up and Waste port is
pointing down (see Figure 6 on page 14). Use the included hex key to secure
the diverter valve to the column.
5. Connect all power cords to available grounded, surge-protected outlets.
6. Connect the preassembled 26-inch PEEK 1/16" OD tubing, supplied in the
BioFrac fittings kit, to the Collect port of the diverter valve and to the drophead.
Use the 0.02" ID (orange) tubing for flow rates less than 20 ml/min and the 0.03"
ID (green) tubing for flow rates greater than 20 ml/min.
Note: The diverter valve is rated to a maximum pressure of 30 psi.
If tubing other than that found in the fittings kit is used to plumb the fraction
collector, make sure all tubing lengths are kept to a minimum. This reduces the
delay volume (see Collect Using a Delay Function on page 67) and
backpressure. Tubing choice is dependent on the flow rate and pressure
characteristics of the pumping system. For flow rates above 20 ml/min, use
0.03" ID or 1/8" OD, 0.062" ID tubing (available from most tubing/fitting
suppliers).
7. Attach the 0.03" ID Tefzel tubing, supplied in the fraction collector fittings kit, to
the Waste port of the diverter valve and place the other end into a waste
collection vessel.
Note: If using flow rates greater than 20 ml/min, be sure to use tubing that
has a 0.03" or larger ID.
User Guide | 1 5
3 | System Configuration and Plumbing
8. Attach the tubing from your chromatography system to the Common port on
the diverter valve using either a 1/4-28 fitting or luer to 1/4-28 union. The
BioFrac fittings kit includes 1/4-28 fittings (for 1/16" OD tubing) and luer to
1/4-28 unions.
9. Connect the fraction collector to your chromatography system as described in
the next section, Connecting Instruments and Devices to the BioFrac Fraction
Collector.
Connecting Instruments and Devices to the BioFrac Fraction Collector
The BioFrac fraction collector can be operated in any of the following
configurations.
BioLogic DuoFlow system control. The system controls the operation of
the fraction collector through the system bus. See Connecting to a
BioLogic DuoFlow Chromatography System on page 17 for details.
LP/Econo mode collection with Bio-Rad components such as the BioLogic
LP system and Model EP-1 Econo™ Pump. The BioLogic LP system
Econo pump controls all aspects of fraction collection.
Stand-alone fraction collection (Local mode) with Bio-Rad components,
such as the Econo gradient pump, a BioLogic QuadTec™ detector, a
Model EM-1 Econo UV monitor, and/or a Model 1327 chart recorder.
Note: The fraction collector provides complete control of fraction
collection.
Stand-alone fraction collection with components that are not
manufactured by Bio-Rad, such as a pump, UV monitor, and/or chart
recorder. In Local mode, the fraction collector provides complete control of
fraction collection and can start/stop an external device such as a pump or
chart recorder. In LP/Econo mode, the controller provides complete control
16 | BioFrac Fraction Collector
Connecting Instruments and Devices to the BioFrac Fraction Collector
of fraction collection. Devices not manufactured by Bio-Rad must be
capable of transistor to transistor logic (TTL) control.
Note: Before proceeding, make sure power to each component to be
connected is turned off. Be sure to use a grounded, surge-protected outlet
when plugging in the power cables.
Connecting to a BioLogic DuoFlow Chromatography System
Connect the BioFrac fraction collector to the BioLogic DuoFlow system using
system cables 17, 18, 19, or 21.
Fig. 7. Connecting the fraction collector to a BioLogic DuoFlow chromatography
system.
User Guide | 1 7
3 | System Configuration and Plumbing
To connect the fraction collector to a BioLogic DuoFlow system
1. Connect the bus cable to the connector marked INSTRUMENT BUS on the
base of the fraction collector.
2. Connect the other end of the bus cable to either the INSTRUMENT BUS
connector on the back of the BioLogic DuoFlow workstation or to other
Bio-Rad components that are daisy chained to the BioLogic DuoFlow
workstation by the instrument bus.
3. Place the BioFrac fraction collector in Local mode with the Main screen
displayed (see Main Screen on page 36). Start the BioLogic DuoFlow software
and press System in the manual screen fraction collector dialog box.
Note: The BioFrac fraction collector must be in Local mode and on the
Main screen before the BioLogic DuoFlow system can control it.
Connecting to a BioLogic LP System or to a Model EP-1 Econo Pump
To connect the fraction collector to the BioLogic LP system or Model EP-1 Econo
pump requires system cables 3 and 15. System cable 15 relays the fraction advance
signal to the BioFrac device, and system cable 3 allows the BioLogic LP system or
Model EP-1 Econo pump to control the BioFrac fraction collector’s diverter valve.
Fig. 8. Connecting the fraction collector to a BioLogic LP system and
Model EP-1 Econo pump.
18 | BioFrac Fraction Collector
Connecting Instruments and Devices to the BioFrac Fraction Collector
To connect the fraction collector to the BioLogic LP system or
Model EP-1 Econo pump
1. Connect system cable 15:
a. Connect the cable’s 15-pin D connector to the port labeled I/O on the
fraction collector.
b. Connect the mini-DIN connector to the port labeled Fraction Collector on
the BioLogic LP system or the port labeled
pump.
2. Connect system cable 3:
a. Connect one of the cable’s mini-DIN connectors to the REC port on the
fraction collector.
b. Connect the other mini-DIN connector to the port labeled Diverter Valve on
the BioLogic LP system or the port labeled
pump.
3. If you are connected to a BioLogic LP system, press Collector on the faceplate
and then press the MODEL softkey followed by the BIOFRAC (or 2128) softkey.
4. When prompted to verify that an external valve cable is connected, enter Yes.
on the Model EP-1 Econo
on the Model EP-1 Econo
5. Follow the procedure, To run the fraction collector in LP/Econo mode on
page 75.
User Guide | 1 9
3 | System Configuration and Plumbing
Connecting to the Econo Gradient Pump and a Chart Recorder
The following configuration shows the fraction collector set up with an Econo
gradient pump and a chart recorder. This system allows full use of the fraction
collector’s advanced programming features.
Fig. 9. Connecting the fraction collector, Econo gradient pump, and chart
recorder.
To connect the fraction collector, Econo gradient pump, and chart
recorder
1. Connect the mini-DIN connector to the I/O connector on the Econo gradient
pump.
2. Connect the 15-pin D-connector to the I/O connector on the fraction collector.
3. If a chart recorder is present, connect the cable’s remaining end to the chart
recorder DIN connector. If the chart recorder is not present, place this portion of
the cable aside.
4. If collecting fractions by threshold, connect a detector to the fraction collector
and chart recorder as described in this chapter.
5. Follow the procedures in Chapter 5, Stand-Alone Operation, on page 57.
20 | BioFrac Fraction Collector
Connecting Instruments and Devices to the BioFrac Fraction Collector
Connecting the Model EP-1 Econo and Econo Gradient Pumps
The BioFrac fraction collector can start an Econo gradient pump or
Model EP-1 Econo pump remotely. To make the necessary connections you will
need a BioFrac accessory cable (catalog # 731-8290) and system cable 7 (catalog #
731-8267).
1. Connect the BioFrac accessory cable green/black and blue/white wires to the
system cable 7 blue wire.
2. Connect the BioFrac accessory cable’s orange/black wire to the system cable 7
yellow wire if you are connecting to an Econo gradient pump, or red wire if you
are connecting to a Model EP-1 Econo pump.
3. Trim off all unused wires.
4. Connect the end of the BioFrac accessory cable with the 15-pin D connector to
the I/O port on the BioFrac fraction collector.
5. Connect the system cable 7 mini-DIN connector to the I/O port on the Econo
gradient pump or Model EP-1 Econo pump.
6. With the fraction collector in Local mode, press Run to start the fraction
collector and pump.
7. If you are collecting by volume, be sure to enter the pump flow rate into the
BioFrac device’s flow rate entry field.
Note: Pressing Stop on the BioFrac fraction collector will not stop the Econo
gradient pump run. The EGP program must be stopped from the Econo
gradient pump faceplate.
Connecting to Components Not Manufactured by Bio-Rad
The BioFrac fraction collector is designed to work with a variety of components
from other manufacturers. The BioFrac fraction collector can control remote devices
such as pumps, chart recorders, and UV detectors, and it can accept start/stop
signals from other equipment, such as pumps and UV detectors. The following is a
description of the remote fraction collection control options. To connect the fraction
User Guide | 2 1
3 | System Configuration and Plumbing
collector to another manufacturer’s pump, UV monitor, and/or chart recorder, you
need a BioFrac accessory cable (15-pin to bare wires), system cable 7 (mini-DIN to
bare wires), and 2-wire shielded cable (26 gauge or larger, available from most
electronics or hardware stores). In addition, the pump and chart recorder circuit
logic must be Start/closed circuit = ON; Stop/open circuit = OFF. The UV monitor’s
analog output must be 100 mV or 1 V. (Refer to Appendix B, Rear Panel Connector
Information (page 85), and Appendix C, Specifications (page 91), for details.)
Fig. 10. Connecting the fraction collector to components other than Bio-Rad’s.
To remotely start or stop an external pump from the BioFrac fraction
collector
1. As described in Chapter 5, Stand-Alone Operation (page 57), set the fraction
collector to Local mode. In Local mode, all fraction collection parameters are
programmed on the BioFrac device’s front panel.
2. Connect the BioFrac accessory cable (15-pin to bare wires; see Appendix B,
Rear Panel Connector Information, on page 85) to the I/O port on the back of
the fraction collector.
22 | BioFrac Fraction Collector
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