Zeiss KF 2 ICS User Manual

KF 2

ICS

Transmitted Light Microscope

Operating Manual

Carl Zeiss KF 2

ICS

Knowledge of this manual is required for the operation of the instrument. Would you ther efore please make yourself familiar with the contents of t his manual and pay special att ention to hint s concerning the safe operation of the instrument.

The specifications are subject to change; the manual is not covered by an update ser vice. © Unless expressly authorized, forw arding and duplic ation of t his document , and the utiliz ation and

communication of its contents are not permitted. Violations will entail an obligation to pay compensation.

Issued by:

Carl Zeiss Microscopy

D-07740 Jena Phone: ++49-36 41 64-16 16 Telefax: ++49-36 41 64-31 44 E-mail: micro@zeiss.de Internet: www.zeiss.de/micro

Number of this manual: B 40-028 e Date of issue: 10/97

II B 40-028 e 10/97

KF 2

ICS

Carl Zeiss

CONTENTS

Page

INTRODUCTION .................................................................................................................I

Title page............................................................................................................................I

Copyright........................................................................................................................... II

Contents............................................................................................................................III

Notes on instrument safety................................................................................................IV

Notes on warranty..............................................................................................................V

Overall view.......................................................................................................................VI

1 DESCRIPTION.................................................................................................................1-3

1.1 Terms, intended use........................................................................................................1-3

1.2 Instrument description.....................................................................................................1-3

1.3 Modules..........................................................................................................................1-4

1.4 Technical data.................................................................................................................1-6

2 START-UP.......................................................................................................................2-3

2.1 Installation of the instrument........................................................................................... 2-3

3 OPERATION...................................................................................................................3-3

3.1 Objective selection...........................................................................................................3-3

3.2 Visual overall magnification..............................................................................................3-4

3.3 Eyepiece reticles...............................................................................................................3-4

3.4 Setting the microscope in brightfield................................................................................3-6

3.5 Setting the microscope for phase contrast ........................................................................3-8

3.6 Setting the microscope in darkfield................................................................................3-10

3.7 Photomicrography.........................................................................................................3-11

3.8 Videomicroscopy ...........................................................................................................3-12

3.9 illumination via mirror and condensor 0.9 AS .................................................................3-12

4 CARE, MAINTENANCE AND TROUBLESHOOTING........................................................4-3

4.1 Care and maintenance of the instrument.........................................................................4-3

4.2 Troubleshooting and requesting service............................................................................4-4

ANNEX..........................................................................................................................A-1

List of abbreviations ........................................................................................................ A-3

Physical and technical units............................................................................................. A-4

EC conformity declaration............................................................................................... A-5

Certification in accordance with DIN EN ISO 9001 / DIN EN 46001................................... A-7

B 40-028 e 10/97 III

Carl Zeiss KF 2

Notes on instrument safety

ICS

The KF 2

microscope, including its original accessories, may only be used for the microscope

ICS

applications described in this manual.

The manufacturer cannot assum e any liability for any ot her applications, pos sibly also involv ing individual modules or single parts. This also applies to all service or repair work which is not carried out by authorized service personnel. Furthermore, all guarantee/warranty claims also expire for all those parts which were not directly affected by the repair.

Particular attention must be paid to the following warning notes: The KF 2

microscope was designed, produced and tested in compliance with DIN EN 61010-1

ICS

(IEC 1010-1), Safety requirements for electrical measuring, control and laboratory instruments, and meets the requirements of appendix I of directive 73/ 23/EC and the relevant CSA and UL directives. The instrument meets the requirements of the EC directive 89/336/EC and the EMC legislation of November 9th 1992. This operation manual includes information and warnings which must be observed by the user.

The KF 2

microscope is categorized as Protection Class I and has been allocated the

ICS

protection degree IP 20. The power plug must be inserted in a properly installed socket featuring a grounding contact. The grounding effect must not be made ineffective by an extension cable which does not have a protective ground wire.

Before switching on the instrument, check whether it is suitable for the line voltage present. The voltage is indicated on the underside of the microscope stand. Incorrect voltage settings can destroy the instrument.

If it is established that the protection measures are no longer ef fective, t he instrument must be switched off and safeguarded against inadvertent operation. For the repair of the KF 2

ICS

microscope, please contact the Carl Zeiss Microscopy Service in Germany (see page 4- 6) or your local Carl Zeiss agency.

Dust and dirt can impair the performance of the KF 2

microscope. Therefore, protect the

ICS

instrument against these influences as far as possible. If the microscope will not be used for longer periods of time (more than 6 hours), it m ust be pr ot ect ed using t he dust cover .

IV B 40-028 e 10/97

KF 2

ICS

The instrument must be operated by trained personnel only, who must be aware of the possible danger involved with microscopy and the relevant application.

Carl Zeiss

The KF 2 performance or damaged when handled improperly.

All repairs inside the instrument may only be performed by the Carl Zeiss Microscopy Service in Germany (see page 4-6) or your local Carl Zeiss agency.

Notes on warranty:

The manufacturer guarantees that the instrument has no material and production defects when delivered. You must inform us of any defects immediately and we must do anything to minimize the damage. If the manufacturer is informed of such a defect, he is obliged to remove it; it is his decision whether he does this by re pairing the instr ument or by deliver ing an instrument free of any defect. No guarantee is provided for defects caused by natural wear (wearing parts in particular).

microscope is an optical precision instrument which can be impaired in its

ICS

The instrument manufacturer is not liable for damage caused by f aulty operation, negligence or any other meddling with the instrument, or the use of accessories from other manufacturers. This forfeits all the claims against warranty.

With the exception of the work specified in this manual, no maintenance or repair of the KF 2

may be performed. Repairs may only be performed by Carl Zeiss Microscopy Ser vice staff or

ICS

specially authorized personnel. Should any defect occur with the inst r um ent , please contact the Carl Zeiss Microscopy Service in Germany (see page 4-6) or your local Carl Zeiss agency..

B 40-028 e 10/97 V

Carl Zeiss KF 2

ICS

Overall view of the KF 2

microscope

ICS

VI B 40-028 e 10/97

KF 2

ICS

Carl Zeiss

DESCRIPTION

Contents

1 DESCRIPTION.................................................................................................................1-3

1.1 Terms, intended use ........................................................................................................1-3

1.2 Instrument description.....................................................................................................1-3

1.3 Modules..........................................................................................................................1-4

1.4 Technical data.................................................................................................................1-6

List of illustrations

Fig. 1-1 KF 2

ICS

microscope equipment........................................................................................1-4

B 40-028 e 10/97 1-1

Carl Zeiss KF 2

ICS

1-2 B 40-028 e 10/97

KF 2

ICS

Carl Zeiss



DESCRIPTION

1.1 Terms, intended use

Manufacturer's name:KF 2 The KF 2

microscope is a universal microscope for the visualization of fine structures and forms in

ICS

microscope

ICS

biology and medicine. These are typical application fields of this microscope:

Schools, universities

Medicine (for diagnosis)

Quality inspection

1.2 Instrument Description

The KF 2

microscope is a small, compact instrument which permits high-performance transmitted-

ICS

light microscopy at little effort.

Major features:

Sturdy, compact stand

Coaxial coarse and fine drive

Microscope stage with optional stand configuration:

Fixed stage with spring clamps or mechanical stage 75  25 with specimen holder L or mechanical

stage 75  25 with specimen holder R Condenser 0.9 AS with aperture diaphragm for low- pow er obser vat ion

4-position nosepiece with W 0.8 thread

ICS objectives

Binocular tube 45°/20 ICS

Built-in illuminator with 6V 10W halogen lamp

Power unit with integrated, s t abiliz ed pow er s upply

12 V battery operation on request

Illumination using external light source possible via m irror

B 40-028 e 10/97 1-3

Carl Zeiss KF 2

1.3 Modules

ICS

Fig. 1-1 KF 2

microscope equipment

ICS

1-4 B 40-028 e 10/97

KF 2

ICS

Carl Zeiss

KF 2

microscope equipment Cat. No.

ICS

KF 2

microscope stand with 4-position W 0.8 nosepiece,

ICS

condenser 0.9 AS with aperture diaphragm and addit ional lens for low-power observation, built- in illum inator, stabilized power supply, power cable with safety plug or American flat plug, 2 halogen lamps 6V/10W and instrument protection cover.

1.1 KF 2

stand equipment with fixed stage and 2 spring clamps

ICS

450719-9901-000 (230 V) KF 2

stand equipment with fixed stage and 2 spring clamps

ICS

450720-9901-000 (115 V)

1.2

KF 2

stand equipment with mechanical stage 75  25 L and

ICS

450713-9901-000 specimen holder with spring clip R (230 V) KF 2

stand equipment with mechanical stage 75  25 L and

ICS

450714-9901-000 specimen holder with spring clip R (115 V)

2 Binocular tube 45°/20 ICS 452928-0000-000

3.1

3.2

Eyepiece PL 10/18 Br. foc. Eyepiece PL 10/18 Br.

444132-9901-000

444131-9901-000

4.1

4.2

4.3

4.4

4.5

CP-"Achromat" objective 5/0.12 CP-"Achromat" objective 10/0.25 CP-"Achromat" objective 40/0.65 CP-"Achromat" objective 100/1.25 Oil CP-"Achromat" objective 40/0.65 Ph 2 (alternative to 440950)

Ph 2 equipment Diopter d = 30 mm

5 Instrument protection cover

440920-0000-000

440930-0000-000

440950-0000-000

440980-0000-000

440951-0000-000

465214-0000-000

444020-0000-000

B 40-028 e 10/97 1-5

Carl Zeiss KF 2

ICS

1.4 Technical Data (1) Dimensions

Dimensions (width  depth  height) ......................................................................185  185  400 mm

(2) Weight

Stand equipment.................................................................................................................approx. 3 kg

Binocular tube 45°/20 ICS....................................................................................................approx. 1 kg

KF 2

microscope, complete............................................................................................. approx. 5 kg

ICS

(3) Ambient conditions Storage and transport (in packaging):

Permissible ambient temperature.......................................................................................-40 to +70° C

Permissible relative humidity (without condensation).............................................................max. 100 %

Operation:

Permissible ambient temperature......................................................................................+10 to +40° C

Permissible relative humidity (without condensation)...............................................................max. 90 %

(4) Operating data

Category of use.................................................................................................................. closed rooms

Protection class................................................................................................................................ SK 1

Protection degree............................................................................................................................IP 20

Electrical safety.................................................................in compliance with DIN EN 61010 (IEC 1010-1)

including CSA and UL directives

Pollution degree...................................................................................................................................2

Excess voltage category.........................................................................................................................II

Radio interference suppression.......................................................in accordance with EN 55011, Class B

Line voltage.......................................................................................................100 to 127 VAC (10%)

or 220 to 240 VAC (10%)

Line frequency...........................................................................................................................50/60 Hz

Label of voltage range.......................................................................................on instrument underside

max. power consumption..............................................................................................................35 VA

battery supply voltage ................................................................................................................12 V DC

(5) Light source

Built-in illuminator system with halogen lamp............................................................................6 V, 10W

Lamp voltage (variable).......................................................................................................0 to max. 6 V

(6) Opto-mechanical data

Stand with objective focusing..........................................................coaxial coarse (fine drive, lift: 15 mm

coarse drive: 1 rotation = 4 mm, fine drive: step- down ratio 1:10

Objective change..................................................................... manually via 4-position nosepiece W 0.8"

Largest possible eyepiece field...................................................................................................... 20 mm

Largest possible object field........................................................................................................... 5 mm

Binocular tube................................................. interpupillary distance can be set between 55 and 75 mm

1-6 B 40-028 e 10/97

+ 28 hidden pages