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The “How Do…” Guide
to AxioVision
Author:
Brian Svedberg
Image Analysis Specialist
Carl Zeiss MicroImaging, Inc.
How do I grab a single image?
How do I grab a single image?
•Click on the Camera icon 
to switch to the camera property page.
•Make sure light is directed to the camera, and click the Live icon 
.
•Click the Measurement icon 
. The exposure will automatically adjust.
•Fine tune the exposure by adjusting the exposure slider or using the up and down arrows.
•In the case of a color camera click the Interactive button, which will change to picking... and click on an area that’s white in the live image.
Æ
•Click the Snap icon 
to capture the image.
Carl Zeiss |
The "How Do..." Guide to AxioVision - 02.2002 |
1 |
How do I use the archive?
How do I use the archive?
•To create a new archive go to the menu at the top and go to File Æ New and the New dialog box will open.
•Go to the Archives tab.
•Enter a name for your archive in the space provided on the right and click OK.
•To open an existing archive click on the Open Archive icon 
.
•To add an image to the archive make sure the image you want to add is active and click the Add Active Image to Archive icon 
.
•Add any information you would like to store with the image into the archive form and click OK.
Carl Zeiss |
The "How Do..." Guide to AxioVision - 02.2002 |
2 |
How do I create a template for multichannel images?
How do I create a template for multichannel images?
•Go to File Æ New and the New dialog box will open up.
•Go to the Images tab.
•Select the Multichannel template and click OK.
•A blank window will open with the word Template in the top left.
•Proceed to the Acquisition page by clicking on the Acquisition icon 
.
•Click on the Color tab. There are three default channels (DAPI, FITC, Rhodamine).
Carl Zeiss |
The "How Do..." Guide to AxioVision - 02.2002 |
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How do I create a template for multichannel images?
•Click on the Add and Remove buttons to get to the number of channels you want for your template.
•To change the filters click on the Color column and select from the list provided or go to Edit Custom Colors to create your own.
•To change the exposure time for each channel highlight the current time and enter a new one. Hit Tab on the keyboard for the change to take effect.
Carl Zeiss |
The "How Do..." Guide to AxioVision - 02.2002 |
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How do I create a template for multichannel images?
•If you have a motorized microscope you can save a setting with each channel. (To create microscope setting see page 17).
•Highlight the first channel by click on the gray square to the left of the enable column.
•Go to the Microscope Settings area at the bottom of the page and choose the setting corresponding to the highlighted channel from the pull-down menu under
During Acquisition.
•Do the same for under Between Acquisition (usually Shutter closed).
•Repeat this for each channel.
•Once you’ve set the filter, exposure, and microscope setting (if applicable) go to File- Æ Save as Template and enter a name (i.e. DAPI&FITC&Rhodamine) and click OK.
Carl Zeiss |
The "How Do..." Guide to AxioVision - 02.2002 |
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How do I use a template to grab a multichannel image?
How do I use a template to grab a multichannel image?
•Open your template for the image you would like to grab by going to File Æ New or clicking on the New icon 
.
•The New dialog box will open with a list of all templates. Highlight the template you want to use by clicking on it once, and enter a name for your image in the space provided on the right side. Click OK.
•The blank template window will open.
•Go to the Acquisition page by clicking on the Acquisition icon 
.
•Click on the Color tab.
•Highlight the first channel by clicking on the gray box to the left of the enable column.
•Make sure the microscope is set-up correctly for the corresponding channel and light is directed to the camera.
Carl Zeiss |
The "How Do..." Guide to AxioVision - 02.2002 |
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