Operating Manual
Axiovert 200 / Axiovert 200 M
Inverted Microscopes
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INTRODUCTION |
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Carl Zeiss |
Copyright |
Axiovert 200 |
Knowledge of this manual is required for the operation of the instrument. Would you please therefore make yourself familiar with the contents of this manual and pay special attention to hints concerning the safe operation of the instrument.
The specifications are subject to change; the manual is not covered by an update service.
©Unless expressly authorized, forwarding and duplication of this document, and the utilization and communication of its contents are not permitted. Violations will entail an obligation to pay compensation.
All rights reserved in the event of granting of patents or registration of a utility model.
Issued by: |
Carl Zeiss |
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Light Microscopy |
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P.O.B. 4041 |
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37030 Göttingen |
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GERMANY |
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Phone : |
++49 551 5060 660 |
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Telefax: |
++49 551 5060 464 |
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Internet : |
www.zeiss.de/micro |
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E-Mail: |
micro@zeiss.de |
Number of this manual: B 40-080 e
Date of issue: 30.03.2001
0-2 |
B 40-080 e 03/01 |
|
INTRODUCTION |
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Axiovert 200 |
Contents |
Carl Zeiss |
CONTENTS
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page |
INTRODUCTION ........................................................................................................ |
0-2 |
Copyright ................................................................................................................... |
0-2 |
Contents..................................................................................................................... |
0-3 |
Notes on instrument safety.......................................................................................... |
0-7 |
Notes on warranty .................................................................................................... |
0-10 |
Overall view of the Axiovert 200................................................................................ |
0-11 |
Overall view of the Axiovert 200 M............................................................................ |
0-11 |
Microscopy in transmitted-light brightfield in a few steps ........................................... |
0-12 |
CHAPTER 1 |
INSTRUMENT DESCRIPTION ..................................................................................... |
1-3 |
1.1 |
Name and intended application ................................................................................... |
1-3 |
1.2 |
Instrument description and main features .................................................................... |
1-5 |
1.3 |
Microscope configurations and modules ...................................................................... |
1-6 |
1.4 |
Objectives ................................................................................................................. |
1-10 |
1.5 |
Eyepieces .................................................................................................................. |
1-12 |
1.6 |
Condensers............................................................................................................... |
1-12 |
1.7 |
Specimen stages and mounting frames...................................................................... |
1-13 |
1.8 |
Binocular tubes ......................................................................................................... |
1-13 |
1.9 |
Technical Data .......................................................................................................... |
1-14 |
B 40-080 e 03/01 |
0-3 |
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INTRODUCTION |
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Carl Zeiss |
Contents |
Axiovert 200 |
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page |
CHAPTER 2 |
START-UP.................................................................................................................. |
2-5 |
2.1 |
Unpacking and installation of the microscope............................................................. |
2-5 |
2.2 |
Attachment of binocular (photo) tube ......................................................................... |
2-6 |
2.2.1 |
Inserting the eyepieces and the centering telescope .................................................... |
2-6 |
2.2.2 |
Inserting the eyepiece reticle ....................................................................................... |
2-7 |
2.3 |
Attachment of transmitted-light illumination ............................................................... |
2-8 |
2.3.1 |
Attachment of carrier for transmitted-light illumination (100 W) .................................. |
2-8 |
2.3.2 |
Attachment of carrier for transmitted-light illumination (30 W) .................................... |
2-8 |
2.4 |
Screw in objectives ..................................................................................................... |
2-9 |
2.5 |
Attachment of microscope stages ............................................................................. |
2-10 |
2.5.1Attachment of mechanical stage 130x85 R/L and mounting frame
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for mechanical stage (K)............................................................................................ |
2-10 |
2.5.2 |
Attachment of scanning stage................................................................................... |
2-10 |
2.5.3Attachment of specimen stage 250x230, object guide and mounting frame
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for object guide (M) .................................................................................................. |
2-11 |
2.5.4 |
Attachment of heating stage .................................................................................... |
2-12 |
2.5.5 |
Attachment of gliding stage Z ................................................................................... |
2-12 |
2.6 |
Attachment of condensers ........................................................................................ |
2-13 |
2.6.1 |
Condensers for the Axiovert 200............................................................................... |
2-13 |
2.6.2 |
Condensers from the Axioplan 2 imaging / Axioskop 2 product line ........................... |
2-13 |
2.6.3 |
Changing the DIC prism in the condenser turret ........................................................ |
2-14 |
2.7 |
Reflector turret ......................................................................................................... |
2-15 |
2.7.1 |
Attachment of reflector turret................................................................................... |
2-15 |
2.7.2 |
Equipment of reflector turret .................................................................................... |
2-15 |
2.7.3 |
Changing the filter set in the FL reflector module ...................................................... |
2-16 |
2.7.4 |
Changing the beam splitter in the FL reflector module ............................................... |
2-16 |
2.8 |
Connection to the line .............................................................................................. |
2-18 |
2.9 |
Interfaces of the Axiovert 200 M ............................................................................... |
2-19 |
2.10 |
Switch microscope and ebq 100 dc power supply on and off..................................... |
2-19 |
2.11 |
Equipotential bonding terminals ................................................................................ |
2-19 |
2.12 |
HAL 100 halogen illuminator..................................................................................... |
2-20 |
2.12.1 |
Change / attachment of the HAL 100 halogen lamp .................................................. |
2-20 |
2.12.2 |
Coarse alignment of halogen illuminator ................................................................... |
2-21 |
2.12.3 |
Attachment of halogen illuminator............................................................................ |
2-21 |
2.12.4 |
Fine alignment of halogen illuminator ....................................................................... |
2-22 |
0-4 |
B 40-080 e 03/01 |
|
INTRODUCTION |
|
Axiovert 200 |
Contents |
Carl Zeiss |
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page |
2.13 |
Illuminator N HBO 103 .............................................................................................. |
2-23 |
2.13.1 |
Attachment / change of the HBO 103 W/2 mercury pressure short-arc lamp............... |
2-23 |
2.13.2 |
Coarse alignment of illuminator N HBO 103............................................................... |
2-25 |
2.13.3 |
Attachment of illuminator N HBO 103 ....................................................................... |
2-26 |
2.13.4 |
Fine adjustment of illuminator N HBO 103 using the adjusting aid.............................. |
2-27 |
2.14 |
Attachment of Aqua Stop ......................................................................................... |
2-28 |
CHAPTER 3 |
OPERATION............................................................................................................... |
3-3 |
3.1 |
Axiovert 200 (manual) ................................................................................................. |
3-4 |
3.1.1 |
Operation and function controls on the Axiovert 200 (manual)..................................... |
3-4 |
3.1.2 |
Switching on and basic settings on the Axiovert 200 (manual).................................... |
3-14 |
3.2 |
Axiovert 200 M (motorized)....................................................................................... |
3-16 |
3.2.1 |
Operation and function controls on the Axiovert 200 (motorized)............................... |
3-16 |
3.2.2 |
Switching on and basic settings on the Axiovert 200 M (motorized) ........................... |
3-23 |
3.3 |
Illumination and contrasting techniques..................................................................... |
3-28 |
3.3.1 |
Setting of transmitted-light brightfield for KÖHLER illumination.................................. |
3-28 |
3.3.2 |
Setting of transmitted-light phase contrast ................................................................ |
3-33 |
3.3.3 |
Setting of differential interference contrast (DIC) in transmitted light.......................... |
3-35 |
3.3.4 |
Setting of VAREL contrast in transmitted light............................................................ |
3-38 |
3.3.5 |
Setting of fluorescence contrast in reflected light ....................................................... |
3-40 |
3.4 |
Documentation ......................................................................................................... |
3-43 |
3.4.1 |
Image orientation of camera ports............................................................................. |
3-43 |
3.4.2 |
Photomicrography with SLR camera........................................................................... |
3-45 |
3.4.3 |
Photomicrography using a digital camera and videomicroscopy .................................. |
3-46 |
B 40-080 e 03/01 |
0-5 |
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INTRODUCTION |
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Carl Zeiss |
Contents |
Axiovert 200 |
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page |
CHAPTER 4 CARE, MAINTENANCE, TROUBLESHOOTING AND SERVICE |
.................................... 4-3 |
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4.1 |
Care ........................................................................................................................... |
4-3 |
4.2 |
Maintenance .............................................................................................................. |
4-4 |
4.2.1 |
Performing checks ...................................................................................................... |
4-4 |
4.2.2 |
Changing the fuses on the microscope........................................................................ |
4-4 |
4.2.3 |
Changing the fuses on the ebq 100 dc power supply .................................................. |
4-5 |
4.3 |
Service........................................................................................................................ |
4-6 |
ANNEX ...................................................................................................................... |
A-1 |
List of abbreviations .................................................................................................... |
A-3 |
List of key words......................................................................................................... |
A-5 |
Certification in accordance with DIN ISO 9001 / EN 46001 |
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EC Declaration of Conformity |
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0-6 |
B 40-080 e 03/01 |
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INTRODUCTION |
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Axiovert 200 |
Notes on instrument safety |
Carl Zeiss |
Notes on instrument safety
The Axiovert 200 / Axiovert 200 M microscopes were designed, produced and tested in compliance with DIN 61010-1 (IEC 1010-1), Safety requirements for electrical measuring, control and laboratory instruments, and meet the requirements of appendix I of directive 73/23/EC and the relevant CSA and UL directives. The instruments meet the requirements of the EC directive 89/336/EC and the EMC legislation of November 9th 1992. This operation manual includes information and warnings which must be observed by the user.
The following warning and information symbols are used in this manual:
NOTE
This symbol is a warning which you must observe under all circumstances.
CAUTION
This symbol is a warning which indicates a hazard to the instrument or instrument system.
CAUTION
This symbol is a warning which indicates a hazard to the user of the instrument.
CAUTION
Hot surface!
CAUTION
UV radiation is emitted!
CAUTION
Disconnect the instrument from the line before opening it!
B 40-080 e 03/01 |
0-7 |
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INTRODUCTION |
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Carl Zeiss |
Notes on instrument safety |
Axiovert 200 |
The Axiovert 200 microscopes, including original accessories, may only be used for the microscope techniques described in this manual.
Particular attention must be paid to the following notes:
The manufacturer cannot assume any liability for any other applications, possibly also involving individual modules or single parts. This also applies to all service or repair work which is not carried out by authorized service personnel. Furthermore, this forfeits all the claims against warranty
The power plug must be inserted in a socket featuring a grounding (earth) contact. The grounding effect must not be made ineffective by an extension cable which does not have a protective ground wire.
If it is established that the protection measures are no longer effective, the instrument must be switched off and safeguarded against inadvertent operation. For repair of the instrument, contact the Carl Zeiss microscope service in Germany (see page 4-6) or your local Carl Zeiss agency.
The Axiovert 200 microscopes are not equipped with any special devices for protection from substances which are corrosive, toxic, radioactive or otherwise hazardous to health. All the legal regulations for accident prevention, particularly those in the respective countries, must be observed when handling such substances.
Before switching on the power unit for the HBO 50/100, check whether it is suitable for the line voltage present.
Always disconnect the instrument from the line before opening the instrument and before changing the fuses.
Make sure to use only fuses of the rated power required. The use of makeshift fuses and the short-circuiting of the fuse holders are not permitted.
0-8 |
B 40-080 e 03/01 |
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INTRODUCTION |
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Axiovert 200 |
Notes on instrument safety |
Carl Zeiss |
Gas discharge lamps, e.g. HBO 100, emit ultraviolet radiation which can cause burns on the eyes and skin. Therefore, never look directly into the light of these lamps and avoid direct, unprotected incidence of their light on your skin. When using the microscope, always use the protective devices belonging to the instrument (e.g. special attenuation filters). When hot, gas discharge lamps are under high internal pressure and must therefore be changed when cooled down by using protective gloves and goggles (for detailed information, please see the operating instructions B 40-065 e).
When fluorescence filters are used, the filter protecting from heat emitted by the microscope illuminator must not be removed, since fluorescence filters are sensitive to heat and their function might therefore be impaired.
Placing objects against or covering ventilation slats can lead to a build-up of heat which will damage the instrument and, in extreme cases, cause a fire. Always keep the ventilation slats clear and make sure that no objects enter the instrument through the ventilation slats.
Avoid touching the hot lamp housings. Always pull the power plug before changing the lamps and allow the instrument to cool down for approx. 15 mins.
Dust and dirt can impair the performance of the instrument. Therefore, the instrument must be protected against these influences as far as possible, and covered with the dust cover if it is not used for longer periods of time. Always check whether the instrument is switched off before you cover it.
The instruments may only be operated by trained personnel who must be aware of the possible danger involved with microscopy and the relevant application.
The Axiovert 200 microscopes are opto-mechanical precision instruments which can be impaired in their performance or damaged when handled improperly.
B 40-080 e 03/01 |
0-9 |
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INTRODUCTION |
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Carl Zeiss |
Notes on warranty |
Axiovert 200 |
Notes on warranty
The manufacturer guarantees that the instrument has no material and production defects when delivered. You must inform us of any defects immediately and we must do anything to minimize the damage. If the manufacturer is informed of such a defect, he is obliged to remove it; it is his decision whether he does this by repairing the instrument or by delivering an instrument free of any defect. No guarantee is provided for defects caused by natural wear (wearing parts in particular) and improper use.
The instrument manufacturer is not liable for damage caused by faulty operation, negligence or any other meddling with the instrument, particularly the removal or replacement of instrument components, or the use of accessories from other manufacturers. This forfeits all the claims against warranty.
With the exception of the work specified in this manual, no maintenance or repair of the microscopes may be performed. Repairs may only be performed by Carl Zeiss service staff or specially authorized personnel. Should any defect occur with the instrument, please get in touch with the Carl Zeiss microscopy service in Germany (see page 4-6) or your local Zeiss agency.
0-10 |
B 40-080 e 03/01 |
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INTRODUCTION |
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Axiovert 200 |
Overall view of the Axiovert 200 and Axiovert 200 M |
Carl Zeiss |
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B 40-080 e 03/01 |
0-11 |
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INTRODUCTION |
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Carl Zeiss |
Microscopy in transmitted-light brightfield in a few steps |
Axiovert 200 |
Microscopy in transmitted-light brightfield in a few steps
Before starting to use the Axiovert 200, make sure to read the notes on instrument safety and the chapters entitled "Instrument Description" (Chapter 1) and "Start-up" (Chapter 2).
•Make the microscope ready for operation as described in chapter 2 and switch it on via the On/Off switch (0-2/1).
•Select the objective with the lowest magnification (e.g. 10x) on the nosepiece (0-2/2). Set factor 1x on the setting wheel (0-2/4) of the Optovar turret.
•Open the luminous-field diaphragm or the aperture diaphragm completely by pulling lever (0-2/16) to the front until stop or by turning the setting wheel (0-2/20) to the front until stop.
•Turn the setting ring (0-2/19) to move the condenser turret in position H for brightfield (or DIC).
•Move reflector turret (0-2/5, if available) into the position without filter combination via the setting ring.
•If required, remove analyzer slider (0-2/3) or switch to free light path.
•Turn setting wheel for Sideport right / left / vis (0-2/22) to position 100 % vis (visual ).
• Turn setting knob for Frontport / Baseport / vis (0-2/23) to position 100 % vis ( VIS ).
•Set beam splitting ratio to 100 % vis (0-2/10) on the tube. Switch off the Bertrand lens (if available). Move combined rotary / slider knob (0-2/9) to position 100 % vis ().
•Place a high-contrast specimen on the microscope stage (0-2/21). Adjust the binocular component.
•Use the coarse / fine focusing drive (0-2/6) to focus on the selected detail of the specimen. Should no light be visible in the eyepieces, switch on the halogen illuminator via the HAL on / off switch (0-2/7).
• |
• |
Fig. 0-1 Diaphragm settings in transmitted-
light brightfield according to •
KÖHLER
Use the toggle switch (0-2/8) to set the light intensity to comfortable brightness.
Close luminous-field diaphragm (0-2/16) until it is visible in the field of view, even if not in focus (0-1/A).
Focus on the edge of the luminous-field diaphragm (0-1/B) by moving the condenser (0-2/17) vertically.
•Center (0-1/C) luminous-field diaphragm via the centering screws (0-2/15 and 18) and open it until the edge of the diaphragm just disappears from the field of view (0-1/D).
•Remove one eyepiece from the eyepiece tube (or swing in Bertrand lens) and set aperture diaphragm (0-2/20) to approx. 2/3 of the diameter of the objective exit pupil (0-1/E). Optimum contrast setting is dependent on the respective specimen.
•Insert the eyepiece again (or swing out Bertrand lens) and refocus, if required, via the fine drive.
•After the microscope has been set to transmitted-light brightfield in this way, changing to this special contrasting technique is now possible (see chapter 3 of this manual).
0-12 |
B 40-080 e 03/01 |
|
INTRODUCTION |
|
Axiovert 200 |
Microscopy in transmitted-light brightfield in a few steps |
Carl Zeiss |
1 |
On / Off switch |
10 |
Turning or sliding knob for |
18 |
Centering screw for condenser |
2 |
Objective nosepiece |
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Bertrand lens and manual shutter |
19 |
Turret disk of condenser |
3 |
Compartment for slider |
11 |
Binocular tube component |
20 |
Setting wheel for aperture |
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Analyzer |
12 |
Setting ring of the eyepiece |
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diaphragm on the condenser |
4 |
Setting wheel for Optovar turret |
13 |
Eyepiece |
21 |
Microscope stage |
5 |
Reflector turret |
14 |
Polarizer D with 2-position filter |
22 |
Compartment for aperture |
6 |
Focusing drive coarse / fine |
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changer |
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diaphragm slider |
7 |
HAL on / off switch |
15 |
Centering screw for condenser |
23 |
Setting wheel for Sideport |
8 |
Toggle switch for illumination |
16 |
Setting lever for luminous-field |
24 |
Setting knob for Frontport / |
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intensity |
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diaphragm |
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Baseport |
9 |
Turning or sliding knob for |
17 |
Setting knob for vertical |
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vis / doc beam splitting |
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adjustment of the condenser |
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Fig. 0-2 Axiovert 200
B 40-080 e 03/01 |
0-13 |
|
INTRODUCTION |
|
Carl Zeiss |
Microscopy in transmitted-light brightfield in a few steps |
Axiovert 200 |
0-14 |
B 40-080 e 03/01 |
|
INSTRUMENT DESCRIPTION |
|
Axiovert 200 |
Contents / List of illustrations |
Carl Zeiss |
INSTRUMENT DESCRIPTION
Contents |
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1 |
INSTRUMENT DESCRIPTION .......................................................................................... |
1-3 |
1.1 |
Name and intended application........................................................................................ |
1-3 |
1.2 |
Instrument description and main features......................................................................... |
1-5 |
1.3 |
Microscope configurations and modules........................................................................... |
1-6 |
1.4 |
Objectives...................................................................................................................... |
1-10 |
1.5 |
Eyepieces....................................................................................................................... |
1-12 |
1.6 |
Condensers ................................................................................................................... |
1-12 |
1.7 |
Specimen stages and mounting frames .......................................................................... |
1-13 |
1.8 |
Binocular tubes.............................................................................................................. |
1-13 |
1.9 |
Technical Data ............................................................................................................... |
1-14 |
List of illustrations |
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Fig. 1-1 |
Microscope configurations and modules (sheet 1) ............................................................ |
1-6 |
Fig. 1-2 |
Microscope configurations and modules (sheet 2) ............................................................ |
1-7 |
Fig. 1-3 |
Microscope configurations and modules (sheet 3) ............................................................ |
1-8 |
Fig. 1-4 |
Microscope configurations and modules (sheet 4) ............................................................ |
1-9 |
B 40-080 e 03/01 |
1-1 |
|
INSTRUMENT DESCRIPTION |
|
Carl Zeiss |
Contents / List of illustrations |
Axiovert 200 |
1-2 |
B 40-080 e 03/01 |
|
INSTRUMENT DESCRIPTION |
|
Axiovert 200 |
Name and intended application |
Carl Zeiss |
1 |
INSTRUMENT DESCRIPTION |
1.1Name and intended application
Manufacturer's name: |
Inverted microscope for transmitted light and epifluorescence |
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Brief name: |
Axiovert 200 |
(manual version) |
|
Axiovert 200 M |
(motorized version) |
The Axiovert 200 microscopes fit in the product family of inverted transmitted-light microscopes as follows:
Laboratory microscopes |
Research microscopes |
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Axiovert 25 |
− |
Axiovert 200 |
− |
Axiovert 25 C |
− |
Axiovert 200 M |
−Axiovert 25 CFL
The Axiovert 200 microscopes are universally applicable inverted microscopes and are mainly used for the examination of cell and tissue cultures and of sediments in culture flasks, Petri dishes, microtiter plates, etc. in transmitted and reflected light.
The Axiovert 200 microscopes permit the performance of the transmitted-light techniques brightfield, phase contrast, differential interference contrast and VAREL contrast, and the epi-fluorescence technique.
The microscopes Axiovert 200 and Axiovert 200 M are the basis for scientific microscopic work on living cells.
−The sturdy stand provides attachment possibilities for various tools (micromanipulation), different light sources, temperature control devices, etc.
−Ample space is available for the specimens and the relevant handling systems on account of the inverted design, the LD illumination system of the microscope and the use of fixed stages. This permits the performance of experiments which would not be possible with upright microscopes.
−The design allows the easy attachment of cameras, lasers, specific stages, etc.
B 40-080 e 03/01 |
1-3 |
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INSTRUMENT DESCRIPTION |
|
Carl Zeiss |
Name and intended application |
Axiovert 200 |
Typical fields of application:
Observation of intracellular processes in living cell cultures, cell/cell interactions, motility, growth, measurements of potential, drug detection, microinjection, IVF (in-vitro fertilization), toxicity examinations, patch-clamp technique, ion measurements, digital recording, long-time / time lapse examinations in combination with the automation of processes, z-sectioning, deconvolution, visualization of molecular structures, Fura (Ca measurement), GFP, optical tweezers and scissors, single molecule detection, TIRF ....
Accessories for temperature control and incubation are described in the separate manual B 40-610 d/e, "Incubation systems on the Axiovert".
1-4 |
B 40-080 e 03/01 |
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INSTRUMENT DESCRIPTION |
|
Axiovert 200 |
Instrument description and main features |
Carl Zeiss |
1.2Instrument description and main features
The Axiovert 200 / Axiovert 200 M is available either as manual or as motorized version. The accessory components are part of a modular system.
For documentation purposes, the Axiovert 200 / Axiovert 200 M can be equipped with the maximum of five camera / TV ports in accordance with the customer's requests.
Adaptation possibilities are provided for heating stages, incubators and micromanipulators.
Major instrument features:
−ICS optics for image creation
−high thermal and mechanical stability
−high flexibility in documentation
−improved ergonomic design
−LCD display of instrument parameters
−23 mm field of view
−Light Manager
−modular design for optimum adaptation to the relevant application
−6-position nosepiece, coded
−5-position reflector turret, manual: can be changed on both sides; motorized: can be changed from the right
−5-position or 6-position condenser turret
−3-position Optovar turret
−changeable aperture diaphragm and luminous-field diaphragm sliders in reflected light
−fluorescence shutter
−illuminators: HAL 100 W, HBO 50, N HBO 103, N XBO 75
−Axiovert 200 M, all the major microscope functions are motorized.
B 40-080 e 03/01 |
1-5 |
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INSTRUMENT DESCRIPTION |
|
Carl Zeiss |
Microscope configurations and modules |
Axiovert 200 |
1.3Microscope configurations and modules
Fig. 1-1 Microscope configurations and modules (sheet 1)
1-6 |
B 40-080 e 03/01 |
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INSTRUMENT DESCRIPTION |
|
Axiovert 200 |
Microscope configurations and modules |
Carl Zeiss |
Fig. 1-2 Microscope configurations and modules (sheet 2)
B 40-080 e 03/01 |
1-7 |
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INSTRUMENT DESCRIPTION |
|
Carl Zeiss |
Microscope configurations and modules |
Axiovert 200 |
Fig. 1-3 Microscope configurations and modules (sheet 3)
1-8 |
B 40-080 e 03/01 |
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INSTRUMENT DESCRIPTION |
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Axiovert 200 |
Microscope configurations and modules |
Carl Zeiss |
Fig. 1-4 Microscope configurations and modules (sheet 4)
B 40-080 e 03/01 |
1-9 |
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INSTRUMENT DESCRIPTION |
|
Carl Zeiss |
Objectives |
Axiovert 200 |
1.4Objectives
The objectives are the optical centerpiece of the microscope. The following is an example of how objectives can be labelled:
A-Plan 10× /0.20 HD ∞ /-
Key:
10× objective magnification, with a color ring on the objective being allocated to each magnification step (Zeiss color code)
0.20numerical aperture
HD |
reflected-light brightfield and darkfield objective |
∞infinite tube length
- |
can be used without cover slip (D = 0 mm) or with cover slip thickness D = 0.17 mm |
or |
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0 |
can only be used without cover slip (D = 0 mm) |
0.17 |
can only be used with cover slip thickness D = 0.17 mm |
and |
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Oil |
oil immersion objective |
Ph 2 |
phase contrast objective with a green color ring and phase stop Ph 2 |
Color ring code for objective magnification:
Color ring on |
black |
brown |
red |
orange |
yellow |
green |
light |
dark |
white |
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objective |
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blue |
blue |
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Magnification |
1.25× |
2.5× |
4× ; 5× |
6.3× |
10× |
16× ;20× ; |
40× ; 50× |
63× |
100× |
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factor |
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25× ; 32× |
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150× |
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The objective magnification (e.g. 10x) multiplied with the eyepiece magnification (e.g. 10x) and the Optovar magnification (e.g. 1.6x) results in the visual overall magnification;
example: 10 x 10 x 1.6 = 160x.
The numerical aperture x 1000, e.g 0.20 x 1000 = 200x, is the highest useful magnification, i.e. no further details are resolved above that limit.
In transmitted-light applications, the exact observance of the cover slip thickness of 0.17 mm is all the more necessary the higher the numeric aperture of the objective. Therefore, so-called "corr" objectives can be set for different cover slip thicknesses via a correction ring. For this, a specimen area is searched, and the position of the correction ring where optimum focus and image contrast are obtained is determined (refocusing is always required).
Immersion objectives are always insensitive to differences in cover slip thickness.
When immersion objectives are used, the air between the cover slip and the objective is replaced with a liquid, which is immersion oil in most cases.
1-10 |
B 40-080 e 03/01 |
|
INSTRUMENT DESCRIPTION |
|
Axiovert 200 |
Objectives |
Carl Zeiss |
The following objectives are available for the Axiovert 200 / Axiovert 200 M microscope:
Objective type |
Magnification/ |
Cover slip |
Free working |
Contrasting |
Cat. No. |
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numeric aperture |
thickness D in |
distance A in |
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mm |
mm |
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A-Plan |
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A-Plan |
5x/0.12 |
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a = 9.1 |
ph0 |
000000-1018-589 |
A-Plan |
10x/0.20 |
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a = 4.4 |
ph1 var1 |
000000-1020-863 |
LD A-Plan |
20x/0.30 |
D = 0.6 - 1.4 |
a = 4.3 |
ph1 |
000000-1006-591 |
LD A-Plan |
20x/0.30 |
D = 0.6 - 1.4 |
a = 4.2 |
ph1 var1 |
000000-1006-592 |
LD A-Plan |
32x/0.40 |
D = 0.6 - 1.4 |
a = 3.1 |
ph1 |
000000-1006-593 |
LD A-Plan |
32x/0.35 |
D = 0.6 - 1.4 |
a = 3.1 |
ph1 var1 |
000000-1006-594 |
LD A-Plan |
40x/0.50 |
D = 0.7 - 1.3 |
a = 2.3 |
ph2 |
000000-1006-595 |
LD A-Plan |
40x/0.50 |
D = 0.7 - 1.3 |
a = 2.3 |
ph2 var2 |
000000-1006-596 |
Cover slip cap |
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D = 0.17 - 0.6 |
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000000-1016-757 |
LD Achroplan * |
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Achroplan |
4x/0.10 |
- |
a = 11.1 |
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440020-0000-000 |
Achroplan |
10x/0.25 |
- |
a = 4.8 |
ph1 |
440031-0000-000 |
LD Achroplan |
20x/0.40 |
corr D = 0 - 1.5 |
a = 10.2 |
|
440844-0000-000 |
LD Achroplan |
20x/0.40 |
corr D = 0 - 1.5 |
a = 10.2 |
ph2 |
440845-0000-000 |
LD Achroplan |
40x/0.60 |
corr D = 0 - 2 |
a = 1.8 |
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440864-0000-000 |
LD Achroplan |
40x/0.60 |
corr D = 0 - 2 |
a = 1.8 |
ph2 |
440865-0000-000 |
LD Achroplan |
63x/0.75 |
corr D = 0 - 1.5 |
a = 1.57 |
ph2 |
440861-0000-000 |
Plan-Neofluar |
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Plan-Neofluar |
5x/0.15 |
- |
a = 13.6 |
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440320-0000-000 |
Plan-Neofluar |
5x/0.15 |
- |
a = 13.6 |
ph1 |
440321-0000-000 |
Plan-Neofluar |
10x/0.30 |
D = 0.17 |
a = 5.6 |
|
440330-0000-000 |
Plan-Neofluar |
10x/0.30 |
D = 0.17 |
a = 5.6 |
ph1 |
440331-0000-000 |
Plan-Neofluar |
16x/0.50 Imm |
D = 0.17 |
a = 0.22 |
|
440530-0000-000 |
Plan-Neofluar |
16x/0.50 Imm |
D = 0.17 |
a = 0.22 |
Ph1 |
440531-0000-000 |
Plan-Neofluar |
20x/0.50 |
D = 0.17 |
a = 2.0 |
|
000000-1004-072 |
Plan-Neofluar |
20x/0.50 |
D = 0.17 |
a = 2.0 |
ph2 |
000000-1004-989 |
Plan-Neofluar |
40x/0.75 |
D = 0.17 |
a = 0.5 |
|
440350-9902-000 |
Plan-Neofluar |
40x/0.75 |
D = 0.17 |
a = 0.5 |
ph2 |
440351-9902-000 |
Plan-Neofluar |
40x/1.30 Oil |
D = 0.17 |
a = 0.2 |
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000000-1022-818 |
Plan-Neofluar |
40x/1.30 Oil |
D = 0.17 |
a = 0.2 |
ph3 |
000000-1022-819 |
Plan-Neofluar |
63x/0.95 Korr. |
D = 0.17 |
a = 0.12 |
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440364-0000-000 |
Plan-Neofluar |
100x/1.30 Oil |
D = 0.17 |
a = 0.12 |
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000000-1018-595 |
Plan-Neofluar |
100x/1.30 Oil |
D = 0.17 |
a = 0.12 |
ph3 |
000000-1031-171 |
Plan-Apochromat |
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Plan-Apochromat |
63x/1.40 Oil |
D = 0.17 |
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440760-0000-000 |
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* a refers to d = 1
B 40-080 e 03/01 |
1-11 |
|
INSTRUMENT DESCRIPTION |
|
Carl Zeiss |
Eyepieces / Condensers |
Axiovert 200 |
1.5Eyepieces
The following eyepieces are available for the Axiovert 200 and Axiovert 200 M:
Eyepiece type |
Image angle |
Cat. No. |
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Eyepiece W-PL 10× /23 Br. foc. |
24.7° |
455043-0000-000 |
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Eyepiece W-PL 10× /23 Br. foc. |
24.7° |
000000-1016-758 |
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Centering telescope d = 30 |
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000000-1006-362 |
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1.6Condensers
The following condensers are available for combination with the stage carrier D and the transmitted-light equipment on the Axiovert 200 / Axiovert 200 M:
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Condenser type |
Cat. No. |
Comments |
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LD condenser 0.35, 5 positions: |
000000-1005-844 |
from objective 2.5x |
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H/DIC, Ph0, Ph1, Ph2, Var1/2 |
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LD condenser 0.55, 6 positions: |
000000-1005-848 |
from objective 4x |
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H, Ph1, Ph2, Ph3, DIC, DIC mot. |
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Condenser 0.35, 6 positions: |
000000-1005-845 |
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H/DIC, Ph0, Ph1, Ph2, DIC, DIC |
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Condenser 0.55, 5 positions: |
000000-1005-846 |
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H, Ph1, Ph2, Ph3, Var1/2 |
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Condenser 0.55, 6 positions: |
000000-1005-847 |
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H, Ph1, Ph2, Ph3, DIC, DIC |
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Condenser adapter for condensers 0.8 |
000000-1005-849 |
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and 1.4 |
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Condenser 0.8 H, D 0.66 / 0.8 Ph DIC, |
445445-9901-000 |
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plus factory-aligned: DIC prism III/0.8 |
445485-0000-000 |
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Condenser 0.8 H, DIC, plus factory- |
000000-1087-444 |
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aligned: DIC prism III/0.8 |
000000-1087-445 |
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Condenser 1.4, 6 positions: |
445453-0000-000 |
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H, D, Ph 2x, DIC 2x |
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1-12 |
B 40-080 e 03/01 |
|
INSTRUMENT DESCRIPTION |
|
Axiovert 200 |
Specimen stages and mounting frames / Binocular tubes |
Carl Zeiss |
1.7Specimen stages and mounting frames
The Axiovert 200 microscopes can be equipped with the following specimen stages and mounting frames:
Description |
Cat. No. |
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Specimen stage 250x230 mm with ceramic surface and |
000000-1005-832 |
3-point support |
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Object guide 130x85 right, can be attached on both |
000000-1005-833 |
sides, accepts various mounting frames |
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Object guide 130x85 left, can be attached on both sides, |
000000-1110-991 |
accepts various mounting frames |
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Mechanical stage 130x85 R/L |
000000-1005-834 |
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Mounting frame for object guide (M) |
see price list |
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Mounting frame for mechanical stage (K) |
see price list |
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Gliding stage Z |
471722-0000-000 |
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Scanning stage |
000000-1113-509 |
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1.8Binocular tubes
The following tubes can be used with the Axiovert 200 microscopes:
Description |
Cat. No. |
VIEWING ANGLE |
Light distribution in |
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% |
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FIELD |
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NUMB |
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ER |
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Binocular tube 45°/23 |
000000-1005-827 |
45° / 23 |
100 % |
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Binocular phototube 45°/23, |
000000-1005-828 |
45° / 23 |
100-0, 0-100, 50-50 |
with Bertrand lens and shutter |
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Binocular ergotube 25°/23 |
000000-1005-829 |
25° / 23 |
100 % |
vertically variable, |
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with Bertrand lens and shutter |
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B 40-080 e 03/01 |
1-13 |
|
INSTRUMENT DESCRIPTION |
|
Carl Zeiss |
Technical Data |
Axiovert 200 |
1.9 |
Technical Data |
|
Dimensions (width x depth x height) |
approx. 295 × 805 × 707 mm |
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Stand Axiovert 200.................................................................................... |
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Weight |
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Axiovert 200 without fluorescence equipment ................................................................... |
approx. 26 kg |
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Axiovert 200 M with fluorescence equipment .................................................................... |
approx. 40 kg |
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Ambient conditions |
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Storage and transport (in packaging): |
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Permissible ambient temperature................................................................................... |
-40 to +50 °C |
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Operation: |
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Permissible ambient temperature.................................................................................. |
+10 to +35 °C |
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Permissible relative humidity (without condensation)........................................................... |
max. 80 % |
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Altitude of use ............................................................................................................... |
max. 2000 m |
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Atmospheric pressure......................................................................................... |
800 hPa to 1060 hPa |
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Pollution degree............................................................................................................................... |
2 |
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Operating data |
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Category of use.................................................................................................................. |
closed rooms |
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Protection class....................................................................................................................................... |
I |
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Protection type ................................................................................................................................ |
IP 20 |
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Electrical safety................................................................. |
in compliance with DIN EN 61010 (IEC 1010-1) |
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including CSA and UL directives |
Excess voltage category ......................................................................................................................... |
II |
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Radio interference suppression....................................................... |
in accordance with EN 55011, Class B |
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Voltage ranges: |
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Integrated 12 V, 100 W power unit, stabilized ............................................ |
100 to 240 VAC (± 10 %) |
1-14 |
B 40-080 e 03/01 |
|
INSTRUMENT DESCRIPTION |
|
Axiovert 200 |
Technical Data |
Carl Zeiss |
Line frequency ...................................................................................................................... |
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50 to 60 Hz |
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Power consumption with internal power units |
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Axiovert 200 ........................................................................................................................... |
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235 VA |
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Axiovert 200 M ....................................................................................................................... |
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235 VA |
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and external power units |
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Power supply N XBO 75, 100 ... |
240 V AC ............................................................................... |
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130 VA |
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Power supply N HBO 100, 90 ... |
240 V AC............................................................................... |
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265 VA |
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Fuses in accordance with IEC 127 |
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Axiovert 200 |
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100 ... |
240 V AC .................................................................................... |
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T 4.0 A/H; 250 V; 5 × |
20 mm |
Axiovert 200 M |
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100 ... |
240 V AC .................................................................................... |
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T 4.0 A/H; 250 V; 5 × |
20 mm |
Light sources |
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HBO 50 W/AC mercury pressure short-arc lamp |
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Lamp voltage for lamp type L1 and L2 .................................................... |
L1: 39 ... 45 V/L2: 34 |
... 39 V |
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Power ........................................................................................................................................ |
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50 W |
Average life............................................................................................................................... |
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100 h |
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HBO 103 mercury vapor short-arc lamp......................................................................................... |
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100 W |
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Opto-mechanical data |
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Stand with stage focusing ............................................................................ |
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with coarse drive (5 mm/rot) |
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and fine drive (0.1 mm /rot) |
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Fine scaling 1 µm/scale part |
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Overall lift approx. 10 mm |
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Objective change ................................................................................................ |
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via 6-position nosepiece |
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Objectives |
...................................................................................................... |
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with W 0.8" x 1/36"thread |
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Eyepieces |
.................................................................................................. |
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with plug-in diameter 30 mm |
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and............................................................................................................................ |
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field number 23 |
B 40-080 e 03/01 |
1-15 |
|
INSTRUMENT DESCRIPTION |
|
Carl Zeiss |
Technical Data |
Axiovert 200 |
1-16 |
B 40-080 e 03/01 |