Zeiss AXIO IMAGER USER MANUAL

START-UP Carl Zeiss Initial start-up Axio Imager
3.1.10.2 Motorized stand
Plug connecting plug (3-12/2) of power supply
230 (3-12/3) into the corresponding connector (3-12/1) on the rear side of the stand.
connector (3-12/4) of the power supply 230 and then into a power outlet. The power supply can be connected to a line voltage of 100 … 127 or 200 … 240 VAC, 50/60 Hz. The power unit is set automatically to the line voltage available.
Fig. 3-12 Axio Imager, motorized
3.1.11 HAL 100 halogen illuminator
The HAL 100 illuminator is used as light source for transmitted-light and reflected-light techniques (except fluorescence) on the Axio Imager. Attachment to the reflected-light or transmitted­light socket is performed analogously.
3.1.11.1 Attaching the HAL 100 halogen illuminator
Remove the protective cap from the reflected-
light or transmitted light socket.
Insert the dovetail mount of the lamp housing
(3-13/9) into the corresponding socket (3-13/2 or 3-13/6) and, using the SW 3 ball-headed screwdriver, tighten it with clamping screw (3-13/1 or 3-13/8).
Insert 3-pole lamp plug (3-13/7) into 3-pole
Fig. 3-13 Attaching the HAL 100 halogen
illuminator
The light manager functionality depends on the position of the toggle switch.
12 V/100 W connector (3-13/3 – for reflected light or 3-13/5 - for transmitted light) on the back of the instrument.
Switch the toggle switch for transmitted/
reflected light (3-13/4) to the required position.
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START-UP Axio Imager Initial start-up Carl Zeiss
3.1.11.2 Adjusting the HAL 100 halogen illuminator
(1) Coarse adjustment
Loosen clamping screw (3-13/1 or 3-13/8) and
remove the operational halogen illuminator from the microscope stand.
Switch on microscope as described in Section
4.3.
Direct the light beam to a projection surface
(wall) that is at least 3 m away.
Do not look into the light exit aperture of the illuminator.
Using the SW 3 ball-headed screwdriver turn
adjusting screw (3-14/3) until both images of the lamp filament appear as sharp as possible on the projection surface.
Then, turn adjusting screws (3-14/4 and 5) until
the lamp filament of one image exactly fills the gaps of the reflected filament image (3-14/1).
Fig. 3-14 Adjusting the HAL 100 halogen
illuminator
(2) Fine adjustment
Reattach the microscope illuminator to the microscope stand and lock it with the clamping screw.
Move diffusion disk for reflected or transmitted light out and remove the filter wheels from the
respective slot.
With an objective of 40x, focus on the specimen and search for a free place on the specimen.
Remove eyepiece and, in the pupil image visible in the eyepiece socket, center the lamp filament and
its reflection with adjusting screws (3-14/4 and 5).
Using adjusting screws (3-14/3) optimize the evenness of illumination of the pupil image.
It is advisable to use the adjusting aid (4-1/5) for fine adjustment of the halogen illuminator mounted to the reflected-light socket. After pulling out the adjusting aid, the lamp filament and its reflection can be viewed directly in its viewing glass.
Move the diffusion disk in and reinsert the filter wheels.
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START-UP Carl Zeiss Attachments and conversions Axio Imager
The reflective (coated) side (3-38/3) of the beam splitter has a beveled edge (3-38/1) or corner (3-38/2).
Place the excitation half of the module
(3-37/1) onto the emission half (3-37/4) (retaining pins 3-37/5b and eyelets 3-37/5a mesh with one another). Hold both halves together and turn them back into the installation position.
Re-insert the slotted screws and tighten them
Fig. 3-38 Marks on beam splitter
up.
Finally, affix the adhesive label with the name of
the filter combination to the side of the module.
3.2.10 Replacing the HAL 100 halogen lamp
CAUTION
Hot surface!
You need not remove the lamp housing from the stand to replace the halogen lamp. Do not store the supplied tool for lamp replacement in the lamp housing while the illuminator is operating. The replacement lamp (3-39/8) may remain put on in the lamp housing.
Switch off the microscope as described in
Section 4.3, disconnect plug (3-13/7) from connector12 V/100 W (3-13/3 – reflected light or 3-13/5 – transmitted light) and allow for a cool down of approximately 15 minutes.
Depress unlock button (3-39/3) of halogen
illuminator HAL 100 (3-39/1), fully pull out lamp carrier (3-39/2) and put it down separately.
Depress both spring levers (3-39/5) and remove
the old halogen lamp (3-39/6) upward. Always hold/grasp the halogen lamp by means of the replacement tool (3-39/7), as even traces of grease on the lamp may affect its lifetime.
Depressing both spring levers insert the new
Fig. 3-39 Changing the halogen lamp
lamp in the lamp socket (3-39/4) and release the spring levers.
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START-UP Axio Imager Attachments and conversions Carl Zeiss
Briefly, depress the spring levers once more thus centering the lamp.
Reattach the lamp carrier sliding it in until it snaps in noticeably.
3.2.11 Installing the LED illuminator for transmitted light
The LED illuminator for transmitted light can be installed optionally on the transmitted light port on the rear side of the instrument or on the bottom of the condenser carrier.
The LED illuminator delivers light of constant color temperature independent of the set light intensity.
CAUTION The LED illuminator is a Class 2 laser device. Do not look directly into the LED light.
Fig. 3-40 Installing the LED illuminator
Installing the LED illuminator on the transmitted light port
Loosen clamping screw (3-40/1) on transmitted-light port (3-40/2). Remove the halogen lamp.
Disconnect the illuminator plug from the 12 V/100 W connector (transmitted light) on the back of the
instrument.
Insert adapter optics for LED illuminator (3-40/3) in the transmitted-light port with the slant pointing
down and tighten the clamping screw.
Screw LED illuminator (3-40/4) onto the adapter optics using the provided Allen screws (3-40/5).
Push slider with diffusion disk 10° (3-40/6) into LED illuminator.
Plug the plug of the LED illuminator into the 12 V/100 W connector (transmitted light) on the back of
the instrument.
You can change the color temperature for specimen observation by inserting color temperature filters in the slider with diffusion disk (to be held by retaining ring).
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