Zeiss Axiophot 2 User Manual

Axioplan 2 imaging and Axiophot 2 Universal Microscopes

Operating Manual

Knowledge of

this

manual

is required

for the operation

of the instrument. Please therefore

familiarize

yourselft with

its

conte

and

pay special regard to the sections dealing with the

safe handling of

the

instrument.

Unless

expressly authorized,

forwarding

and duplication

of this document,

utilization

and communication of its conten

are

not permitted. Violations will entail

an obligation

to

pay

compensation.

All

rights reserved in the eventatentsof granting of p

or

registration

of a

utility

model.

It is not

permitted

to

pass on or copy this

document,

or

to use

or essforward

its

conten

prior consent

has

been

provided.

If

this regulation is

not observed, damages

will be claimed.

All patentingd. rights are rese

For further details, please contact:

Carl Zeiss

Microscopy

D 07740	Jena
Phone:	++49 3641	64 16	16
Telefax:	++49 3641	64 31	44
E mail:	[e-mail]
Internet:	www.zeiss.de/micro

Subject to change

B 40 042 e 09/99

Contents

Notes on the safe operation of the instrument. . . . . . . . . . . . . . . . . . . . . . . 3. General . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4. . . . . . . . .

Purpose . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6. . . . . . . . .

Overview and Connections. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7. . . .

Putting into Operation. . . .	. . . . . . . . . . . .	. . . .	.	. .	. . . . . . . . 8. . . .
Stand . . . . . . . . . . .	. . . . . . . . . . . .	. . . .	. .	. .	. . . . . . . . . . .10. . . . . . . . . .
Manual stand. . . . . .	. . . . . . . . . . . .	. . . .	. .	. .	. . . . . . 11. . . . . .
Stand E/Stand mot. . .	. . . . . . . . . . . . .	. . . . . . . . . . . . . . 17. . . . .
Light manager . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21. . . . .
Automatic balancing of	focusing speed and focus	position. . . .	.	. .	. . . . . . . . . . 21. .

Microscope control software AxioVision Control. . . . . . . . . . . . . . . . . . . . 22. . . .

What does AxioVision Control offer?. . . . . . . . . . . . . . . . . . . . . . . . 22. . . .

System requirements. . . . .	.	.	. . . .	. .	. . .	. .	.	.	. .	. .	. .	.	.		. 22. . . . . .
Electrical connections . . . .	.	.	. . . . .	. .	. . .	. .	.	.	.	. . .	. .	.		.	. 23. . . . .
System Overview. . . . . . . .	. . . . . . . . . . . . . . . . . . . . . . . 24. . . . . .
Microscope Components . . . . . . . . . . . . . . . . . . . . . . . . . . .														. . 31. . . . .
Microscope illuminators . . .	.	.	. . . . .	. .	. . .	. .	.	.	.	. . .	. .	.		.	. 32. . . . .

Tubes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36. . . . . . . .
Eyepieces . . . . .	.	. .	.	.	. . . . . .	.	.	.	.	.	. . . . . . . .	.	. .	. . .38. . . . .
Intermediate tubes .	.	. .	.	.	. . . . . .	.	.	.	.	.	. . . . . . . .	.	. .	. . 40. . . . .

Reflector	modules. .	. .	.	.	. . . . . . .	. .	.	.	.	. . . . . .	. .	. .	.	.	43. . . . . .
Objectives	. . . . .	. .	.	.	. . . . . . .	. .	.	.	.	. . . . . .	. .	. .	.	.	46. . . . . .

Stages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48. . . . . . . .

Condensers for Transmitted Light. . . . . . . . . . . . . . . . . . . . . . . . . 52. . . .

Analyzers, compensators, auxiliary objects and DIC sliders. . . . . . . . . . . . . . . . . 57. . Axiophot 2 Photo module. . . . . . . . . . . . . . . . . . . . . . . . . . . . 59. . . .

Microscopy Techniques. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63. . . . . .

Transmitted light brightfield. . . . . . . . . . . . . . . . . . . . . . . . . . . 63. . . . .

Transmitted light darkfield. . . . . . . . .	. . .	. . . .	. .	.	.	. .	. .	.	.	.	64. . . . .
Darkfield illumination in low magnifications. .	. . .	. . . .	. .	.	.	. .	. .	.	.	.	. 65. . . .

Phase contrast. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66. . . . .

DIC Differential interference contrast in transmitted. .light. . . . . . . . . . . . . . . 67. .

Transmitted light polarization Detection of birefringence. . . . . . . . . . . . . . . . 69. . .

Transmitted light polarization Determining theγ directionn of oscillation. . . . . . . . 70. . . .

Transmitted light			polarization Determining and measuring path differences. . . . . . . . . 71.
Transmitted light			polarization		Determining	the	optical characteristics	of. crystals. . .	. . .	72
Incident	light	brightfield. . . . . . . . . . . . . . . . . . . . . . . . . . . . 74. . . . .
Incident	light	darkfield. . .		.	. . . . . .	. .	. . . . . . . . . . .	. . . . .	. 75. . . . .

DIC Differential interference contrast in incident. .light. . . . . . . . . . . . . . . . 76. . .

Incident light polarization Detection of bireflection and reflection pleochroism. . . . . . . 77 Epi Fluorescence . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78. . . . . .

Photomicrography with the Axiophot. .2 . . . . . . . . . . . . . . . . . . . . . 79. . . .

1

B 40 042 e 09/99

Contents

Care, Maintenance. . . . . .	.	. .	. . . .	. . .	. . . . . .	. . . . . . .	.	.82. . . . . . . .
General . . . . . . . .	. .	. .	. . . .	. . .	. . . . . .	. . . . . . .	. . . . .82. . . . . . . . .
Lamp change. . . . . .	. .	.	. . . .	. . . .	. . . . . .	. . . . . . .	.	. 82. . . . . . . . .

Line supply . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .82. . . . . . . .

Changing the fuses. . . . . . . . . . . . . . . . . . . . . . . . . .	. . . . 82. . . . . . . .
Technical Data. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .83. . . . . . . . .
Interface Description. . . . . . . . . . . . . . . . . . . . . . . . . . .	. . . 84. . . . . . . .

Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .87. . . . . . . . . . . . .

List of Illustrations. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .89. . . . . . . .

Certification in accordance with ISO 9001

EC conformity declaration

2

B 40 042 e 09/99

Notes on the safe operation of the instrument

The Axioplan 2 imaging Universal Microscope has been

de

Specimens

hazardous

to

health!

signed

and

tested

in

compliance

with

EN

61010,

part

1

(DIN

The Axioplan 2 imaging is not equipped with any

VDE

0411)

and

IEC

1010 1

and

left

the

factory

in

a

perfect

special

devices

for

protection

against

corrosive,

state

with

regard

to

its safety facilities. To ensure

that

WARNING!

toxic,

radioactive

or

other

substances

hazardous

this re

mains the case and to guarantee the safe operation of the

to

health.

All legal requirements, especially na

equipment, the instructions and warnings given in

this

tional

accident

regulations,

must

be

observed

manual

must

be

observed.

when

handling such

specimens.

The instrument meets the requirements of the EC directive

89/336/EEC

and

the

EMC

legislation

of

November

9th 1992.

Hot

surfaces!

It has been allocated the protection degree IP 20 and

is

cate

Do not touch the hot lamp housing. Always dis

gorized

as

Class

1

Equipment.

The

power

plug

must

be

in

connect

the

power

plug

before

changing

a

lamp

WARNING!

and allow the unit to cool down for approx. 15

serted in a socket featuring a grounding (earth) contact. The

grounding

effect

must

not

be

nullified

by

an

extension

cable

minutes.

which

does

not

have

a

protective

ground

wire.

Thermally

sensitive

fluorescence

filters!

For

your

own

safety

and

for the

protection

of

the

Fluorescence

filters

are

sensitive

to

the

thermal

radiation

of

the

microscope

lamp

and

their

perfor

instrument

against

damage

WARNING!

mance can be permanently impaired by it. There

Axioplan 2 imaging features special protective devices such as

fore, never remove the heat reflecting filter on the

attenuation

filters

to

protect the eyes against intense

radi

microscope

illuminators

when

you

are

working

ation, and stops to protect specimens and objectives against

with

fluorescence

filters.

knocks and mechanical damage. These protective

devices

must be used and must not be removed. You must familiarize

Heat

build6up!

yourself with the protective devices provided by Axioplan 2

Placing

objects

against

or

covering

ventilation

imaging under

all

circumstances.

slots

on

the

microscope

or

its

components

can

WARNING!

lead

to

a

build up

of

heat

which

will

damage

the

Danger

of

crushing!

instrument

and,

in

extreme

cases,

cause

a

fire.

In

stands

with

a

motorized

focusing

drive,

there

is

Therefore,

always

keep

the

ventilation

slots

clear

a

danger

of

crushing

your

hand

between

the

(minimum

distance

15

cm).

WARNING!

stage

carrier

and

stand

base

when

the

stage

is

Always

check

whether

the

microscope

is

switched

lowered.

For

this

reason,

do

not

place

your

hand

off

before

placing

the

dust

cover

over

it.

under

stage

carrier

when

the

stage

is

being

low

ered.

Dust

und

dirt!

Dust

and

dirt

can

impair

the

performance

of

the

Gas

discharge

lamps!

Axioplan 2 imaging. Therefore, protect the micro

In

unfavorable

circumstances

and

with

improper WARNING!

scope

as

far

as

possible

against

these

influences.

use,

gas

discharge

lamps

can

explode,

flinging

Always

use

the

dust

cover

if

you

do

not

intend to

CAUTION!

splinters

of

glass

through

the

air

and

causing

pos

use

the

Axioplan

2

imaging

for

longer

periods

of

sible

injury.

Therefore,

it

is

imperative

that

the

time

(more

than

6

hours).

safety and operating instructions of the manufac

turer

of

the gas

discharge

lamp

be

followed→

(

Operation!

chapter

onCare,

Maintenance),

The

instrument

must

be

operated

by

trained

staff

Gas

discharge

lamps

emit

ultraviolet

radiation

only.

They

must

be

instructed

in

the

hazards

in

which

can

cause

burns

on

the

eyes

and

skin. Never

volved

in

microscopy

and

the

respective

field

of

look

directly

into

the

light

of

these

lamps

WARNING!

and

application.

This

includes

an

awareness

of

the

risk

avoid

direct,

unprotected

incidence of

their

light

of

eye

injury

due

to

intensive

irradiation

by

light.

on your skin. When using the

microscope,

always

use the protective devices belonging to the instru

ment,

e.g.

special

attenuation

filters.

Gas

discharge

lamps

are

contained,

for

example,

in our microscope illuminators HBO 50,

HBO

100

and

XBO

75.

3

B 40 042 e 09/99

General

Note on

exchangeable

components

Note

on power

unit

integrated in

stand

Perfect functioning of the instrument

requires that

you

useThe integrated power unit is used to supply voltage to the

spare parts and components which

are

marketed

and

apmicroscope

illumination, the Axiophot 2 Photo module and

proved by us. In the event of doubt,

please contact our servicethecoded

motorized components. The power unit must not

staff.

The

use

of parts

from

other manufacturers

may

impairbeused

to

supply

voltage to other external power consuming

the performance of the Axioplan

2

imaging

or

indeeddevices.

This can

lead

to overloading

and destruction of the

damage the instrument. The use of

such

parts

is

the powersole

unit.

responsibility

of

the user.

No

other

care,

maintenance

or repair work

must

be

carried

out apart from the activities specified→ inCare,

Maintenance.

More extensive repairs may only be performed

by

our

customer service experts or by specially authorized persons.

Damage to the instrument may otherwise result.

Note:;We would like to expressly emphasize here that any

adjustments

not

described

in

Microscopethe

Components

chapter must only be performed

by

persons

expressly

authorized by us to do

so.

The

Axioplan

2

imaging

is

a

precise

optical

instrument

which

may be impaired in its function or even damaged by inexpert handling.

4

B 40 042 e 09/99

General

The Axioplan 2 imaging is a universal microscope

whichAbbreviationsis

suitable for all applications relevant to light

microscopy. achrIts.

achromatic

modular design, numerous components and wide range ofAL

incident

light

accessories allow the Axioplan 2 imaging to be adapted aplnd.

aplanatic

extended

to

perform

a

large number

of

special

applicationsBr.

suitable

for eyeglass wearers (eyeglasses can be

placed

directly

against

eyepieces)

There is

practically

no

field

of

application

in

light

microscopyC

camera

for which the Axioplan 2 imaging cannot be

used.

However,CMount

camera

mount

users wishing to utilize the motorized and automaticD

diameter

or

coverglass

thickness

functions of Axioplan 2 imaging must take this

into

accountD

darkfield

when choosing the basic stand configuration. You

willDIC

Differential

interference

contrast

already have made this decision before starting to read

DLthis

transmitted

light

manual. But we would nevertheless like to point out thatE Plthe

flat field eyepieces with aspheric correction

configuration

for

which

you

have

opted must

not

necessarilyWD

working

distance

be the definitive one. If you wish to change your Flbasic

fluorescence

configuration

at

a

later

date,

the

possibility

most

certainlyfo

focusable diopter compensation on eyepiece

exists. Our specialist staff will

be

pleased

to provide

you fotwith.

photographic

any

advice

you

may

need.

FT

chromatic

beam

splitter

HAL

halogen

illuminator

or

lamp

The

aim

of

this manual is to describe the

many

possibleHBO

mercury pressure short arc lamp

functions

of

Axioplan 2 imaging. However, the

sheer

numberHD

bright/darkfield

of functions

offered may sometimes lead to a

certain

amountH

brightfield

of

confusion.

Therefore,

if you have any doubts

concerningICS

Infinity Colour corrected System

the

capabilities

of

your

Axioplan

2

imaging,

please

contactKorr

morrection

mount

our subsidiary or ourselves direct. Our address and telephoneLD

long

working

distance

number

are

given

on

the

inside

cover

of

this manual.

LFB

luminous

field

diaphragm

MC

microscope

camera

In the chapter entitledStand you will find the

various

basicMPM

microscope

photometer

versions of the Axioplan 2 imaging stands. Starting with Nthe.A.

numerical

aperture

manual version, this chapter deals with all stand functionsP

photometry

available, up to and including the stand featuring

Phall 1, 2,

3 phase

contrast;

the

numbers refer to the diameter

motorizable

functions

and the

light

manager.

of the ring stop used; with an objective with the

designation Ph 2, you use the ring stop with the

The

chapter Microscope

Components

describes

the

corresponding

designation Ph 2.

operating functions with which you yourself

can

equip

Polr

polarization

extend the Axioplan 2 imaging. Not all parts

described

thereSFZ

field

of

view

number

must be present on the

Axioplan

2

imaging.

However,

ifSIyou

system

integration

are considering adding some of the components to yourSLR

single

lens

reflex

configuration,

the

corresponding

catalog

numbers

are

listed.Stemi

stereomicroscope

SW

wrench

size

The chapterMicroscopy Techniques provides you with in UD

universal

rotary

stage

formation on the basic settings of the microscope,

eVar.g.

VAREL

contrast

KÖHLER illumination adjustment, and contains

instructions vis.

visual

concerning the operation of the microscope for specific apW Pl

wide

angle

flatfield

plications.

XBO

xenon

short arc

lamp

ZBE

intermediate

image

plane

5

B 40 042 e 09/99

Purpose

The Axioplan 2 imaging has been designed as a universalInstallationmi

conditions

croscope

and can

be

used

for

all

areas of light

microscopy,

pro

vided

it

is

configured

and

o

Dust free environment

equipped appropriately. Depending

relative

air humidity

85 %

on

its

equipment,

it

is

used

in

the

following

fields:

o Maximum

o Vibration free

worktop

o transmitted

light

microscopy

o incident

light

microscopy

Specifications concerning power supply, storage temperature

o fluorescence

microscopy

and other

technical

details are

contained in the chapter

o photomicrography

entitled Technical

Data.

o videomicroscopy.

The Axioplan 2 imaging also

performs the contrasting meth

ods

in

transmitted

light

and

incident

light:

odarkfield

ophase contrast

opolarization contrast

odifferential interference contrast.

Motorizable functions					and		the	recognition of coded micro
scope	components			used		permits the performace of applica
tions in such fields as						process		automation		and telepathology.
Here,	the	microscope			is	controlled by a software which acti
vates	the	programmable				CAN BUS and RS 232 interfaces.
Here,	the	microscope			is	controlled by a built in microprocessor
and a	control		software			which		activates	the	standard interfaces
CAN BUS		and	RS	232		C	in	personal	computers.

6

B 40 042 e 09/99

Overview and Connections

The stand	shown in the illustration is equipped with a wide
variety of	functional units. It may therefore differ from the

stand you have purchased. Should you therefore see any

components in the drawing which are						not	present		on	your
own microscope, this does not					mean that you have an
incorrect Axioplan 2 imaging configuration.
Setting and		adjustment	of certain		components			and	modules
of the Axioplan 2 imaging sometimes						require special				tools
which are included in the delivery					package		of	the microscope.
This	particularly concerns		the	SW	3 ball headed screwdriver
and	the SW	1.5 screwdriver		for	Allen	head		screws.

7	8

6

5

4

9 3

10 2

1

1	Stand (base) with complete transmitted light illumination
2	Condenser		with carrier
3	Stage	with		carrier
4	Objective		nosepiece
5	Reflector		turret
6	Tube
7	Stand	(upper		part)	with complete incident light illumination
8	Lamp	for	incident		light
9	Lamp	for	transmitted light
10	ON/OFF switch

Fig. 1 Axioplan 2 imaging (motorized);

7

B 40 042 e 09/99

Putting into Operation

Unpacking and installing the microscope
Normally,		your	microscope is supplied completely assembled
in	a specially		designed container. The Axiophot 2	Photo
module and the notebook required for its control,				plus	any
required intermediate tubes, are packed separately.				The
transport containers include instructions for unpacking the
instrument.
Please make sure that you observe the instructions contained
therein.
Due	to	the complexity of the Axioplan 2 imaging		and	the

necessity to ensure its perfect functioning, it will generally be

installed and		set	up on	site by our		service personnel.
The	Axioplan	2	imaging	is handed over to you in			a state which
will	enable	the	users to attach		all the items listed in the
Microscope		Components chapter				themselves.	The
procedures required are				described.

8

B 40 042 e 09/99

Putting into Operation

Setting

up

the

microscope

The

Axioplan

2

imaging

microscope

features

an

integrated

wide range power unit and can be connected to line voltages

ranging from 100...240 V AC, 50

... 60 Hz. The wide range

power unit sets itself automatically

to the

appropriate

line

voltage.

Check

that all connected components have been

mounted

correctly and

are

sitting

properly.

Set

ON/OFF

switch (Fig10. 1/)

to

positionO.

4

Connect

power

unit

first

to

the

instrument

and

then

to

the

line.

3

Make

the

other

electrical

connections,

such

as

micro

scope

illuminators,

connecting

cable

to

TV

camera,

2

Axiophot

2,

notebook,

etc.

1

5

Note:;All

the

electrical

and electronic

connections

must

be

6

made before switching on the instrument so that they can be

recognized

by

the initialization

routines.

Set

ON/OFF

switch to positionI.

This last setting switches on

the

microscope

and

makes

it

ready to

use

after

approx.

10

s.

When

the

instrument

is

equipped

with

the light manager, this last position is set.

1 Sockets

of

the

integrated

power

unit

for

the

transmitted light

Switch

on

notebook

or

PC (if connected).

illumination

2 Toggle

switch

to

change between transmitted and incident light

3 Sockets

of

the

integrated

power

unit

for

the

incident light

illumination

Switching

off

the

instrument

4 Socket

for

Axiophot

2 Photo module

5 Socket

for

instrument

plug

(line voltage)

Set

ON/OFF

switch

to

positionO.

6 Compartment

for

instrument

fuses

Fig. 2 Axioplan

2

imaging (instrument

back);

9

B 40 042 e 09/99

The components of the lower stand part are available in the following versions:

o condenser turret: manual or motorized o luminous field diaphragm: manual or motorized o filter wheels: manual,

coded or motorized o z focusing drive: manual or motorized

10

Stand

All the stand configurations described

in the following areFeatures and components

common

to

reflected and

trans

usually tailored to the customers special requirements. Formitted light

stand

types:

example,

it

is

possible that

the

stand

configuration

delivered

connection

to

you

includes

only

incident light or

fluorescence

o lamp

equipment

exit

o light

and that transmitted light components

are

therefore

noto power units

for

HAL

100

microscope

illuminator

and

available.

sevenelectronic

control

system

The

same applies to

the

objective

nosepiece,

of

which

components

models

are

available.

o switches,

adjusting

and

display

o mechanical parts for stage focusing

The

following

stand

types

exist

for

the

Axioplan

2

imaging:

of stands starts with the manual

The following description

o Manual

type

model.

On

this

basis, only the

differences

and additional

This stand can only be operated manually. Motorized andfunctions are described for

the

other

stand

models.

codable

functions

cannot

be

easily retrofitted.

o Type

E

Unlike the manual type, this stand is equipped with an

electronic

system

permitting all the appropriately pre

pared

microscope

components (except the z focusing

drive) to be motorized. These functions can also be easily

retrofitted.

o Motorfocus

type

(MOT)

Like type E, plus motorized focusing equipment

Aside from these basic types we also use the following

abbreviations

in

this

manual:

o LM/E

type

Like

type

E,

plus

additional

light

manager

o LM/MOT

type

Like Motorfocus type, plus additional light manager

Upgrading to include motorized functions is possible,

but

requires the stand type E or higher.

Should you intend to upgrade your microscope to include

motorized functions, please get in touch with the Zeiss service

department. Retrofitting is normally possible without

difficulty.

The components of the upper stand part are available in the

following

versions:

o reflector

turret:

manual

or

motorized

o nosepiece:

manual,

coded

or

motorized

o reflected light

shutter:

motorized

B 40 042 e 09/99

Stand

13 14 15 16 1718

19

12	20
	21
11	22
10

9

8 7

6

5 4

3

2 1

1	ON/OFF		switch
2	Light	intensity				control
3	On/Off display of color temperature key
4	Color	temperature					key
5	LED	line		lamp		voltage
6	Focusing			drive
7	Filter	wheel			2
8	Filter	wheel			1
9	Luminous field diaphragm (transmitted light)
10	Light exit of transmitted light equipment
11	Compartment					for	compensators
12	Reflector			turret
13	Analyzer			compartment
14	Centering screws for luminous field diaphragm (incident light)
15	Pushrod		for		luminous field diaphragm (incident light)
16	Slot	for		filter		slider
17	Centering screws for aperture diaphragm (incident light)
18	Pushrod for aperture diaphragm (incident light)
19	Adjustment aid for lamp setting (incident light option)
20	Objective			nosepiece
21	Lowerable			stage			carrier
22	Swinging in/out of diffusion disk (transmitted light)
Fig. 3	Manual			stand

Manual		stand
The	stand shown here may differ from your stand model.
Most	differences will			be present in the upper part of				the stand
i.e.	they concern incident light and polarizing microscopy. If
the	configuration		illustrated		is different	from your	own Axio
plan	2 imaging,		this	does	not necessarily mean		that	an error
has	been	made,	but	is probably due to the stand				equipment
you	have	chosen	for	your	respective	application.

Controls

(The numbers refer to Fig. 3.)

1ON/OFF switch

Position O =		instrument		switched		off
Position I	=	instrument		switched		on
For optical status checking, the						switch lights up in green in
position I	(for defects		chaptersee		Care, Maintenance).
When the Axioplan 2 imaging						is switched	on,	not	only the
HAL 100	microscope		lamp is		supplied, but		also	the	Axiophot

2 Photo module, if mounted, and the motorized internal components.

2Light intensity control

Knob to adjust the light intensity of connected 100 HAL

microscope			illuminators.
The	power		unit	integrated		in	the	stand	is highly	stabilized
against		voltage		fluctuations		and supplies			adjustable	d.c.
voltage		in	the range		from 3 to		12	V. A	yellow LED	line disp
the	set	voltage		range	and	the	light	intensity.

Note:;If the light intensity cannot be adjusted, please check whether the color temperature 4key) is( switched off.

3On/Off display of color temperature key

Lights up	when the color temperature of 3200 K has been
switched	on.

4Color temperature key

Knob to set		the	3200 K	color temperature for photomicro
graphy	using	color	films (artificial light). When switched on,
a green	LED	lights	above	the knob.

A constant color temperature of 3200 K is required for color

photography.	This is		achieved if a fixed d.c. voltage			of 10.5
is supplied	to	the	HAL 100	microscope	illuminator.	Correct
functioning	can	not	be guaranteed if other lamps than the
12 V/100 W	lamps		supplied by	Carl Zeiss	are used.

11

B 40 042 e 09/99

Stand

7, 8

Filter

wheels

for

transmitted

light

9

Luminous6field

diaphragm

Two

rotatable

filter

wheels

(filter

magazine)

with

4

positions

each

are equipped

with

different

filters;

two

different

Wheel

for

the

continuous

setting

of

the

aperture

of

the

models

are

available.

The

wheels,

which

feature

click

stops,

luminous field diaphragm (transmitted light).

are

rotated

into

position.

The

set filters are

marked on

the

wheels.

Light exit of transmitted6light equipment

10

o Filter magazine D 452155600006000

Light

filters

with

dia.

32

mm

can

be

placed

on

the

plane

Filter magazine for general use.

surface

of

the

centering

ring supplied.

The

positions

of

the

two

wheels

can

be

combined

as

required.

Note:;Additional

filters

can

be

inserted

in

the

color glass

carrier

of the

condenser

mount.

Filter

wheel

27) (100 open

position

25

neutral density

filter

0.25

6

neutral density filter 0.06

14

Centering

screws

1.5

neutral density

filter

0.015

To center the luminous field

diaphragms (incident

light)

using

Filter

wheel

18) (100 open

position

SW 3

screws.

6

neutral density filter 0.06

G

wide band interference filter

green

CB

conversion

filter

3200

K ...

5500

K

o Filter magazine D PHOTO 452157600006000

Filter

magazine

for

the

brightness

control

at

a

constant

color

temperature

of 3200 K. The 3200 K color

tempera

ture must be selected. Green LED above the key lights

constantly. The positions of the two filter wheels can be

combined

as

required.

A high current and a correspondingly bright

lamp

are

needed to achieve the color temperature of 3200 K. Filter

wheel 2

can

be

used

to reduce

the

brightness

of

the

lamp

to a "normal degree" more beneficial

to

the

eye.

The

following

brightness

steps

can

be

set:

Filter

wheel

27) ( coarse

steps

100 maximum

brightness

at

3200

K

100

%

6

normal

brightness

at

3200

K

6

%

0.4 low

brightness

at

3200 K

0.4

%

0transmitted light blocked

Filter wheel 18) (fine steps
100 brightness		100	%	of	coarse	step
50	brightness	50	%	of	coarse	step
25	brightness	25	%	of	coarse	step
12	brightness	12	%	of	coarse	step

Conversion or	green filters are additionally inserted	into
the filter mount or laid onto the light exit		of the
transmitted light	illumination.

12

B 40 042 e 09/99

B 40 042 e 09/99

Fig. 4

Stand

5

4

3

2

1

15 Pushrod

For the continuous diameter setting of the luminous field diaphragm (incident light)

Pushed in = open diaphragm Pulled out = closed diaphragm.

6Manual focusing drive

The universal microscope Axioplan 2 imaging is focused								via
coaxial drives on both sides of the stand.
Coarse drive (large knob on the						inside):
1 revolution = approx. 2				mm, 1 increment = mapproxm. . 2
Fine	drive (small	knob	at	the	outside):
Gear	ratio 1:10	(1 revolution			approx.		0.2 mm)
22	Diffusion	disk	(transmitted light)
		Setting		via	right/left		rotation using
		SW 3 ball headed screwdriver
		Diffusion		disk		OFF
		Diffusion		disk		ON
The	diffusion disk	improves		the	homogeneous illumination			of

the object plane. Normally, it is swung into position. It can b

swung	out using	the SW 3 ball headed screwdriver to
increase	the visibility of the lamp filaments during adjustment
of the	microscope	illuminator.

1

Stage centering screw

for

stage

centration (on

both

sides)

21

Stage carrier

2

Screw on cap for spring pin of

stage clamp

specimen stages The

stage

carrier

is

used

to mount the stage and the con

3

Angular

guidance for

attachment

of dovetail

for

4

Spring pin of stage clamp

denser carrier to which the

condenser

is

attached.

The stage

5

Drilled

holes to screw

on

fixed

stages and

scanning

stages

carrier is either fixed to the stand

or

removable.

The

non

rotating mechanical

stage and the

scanning stage are fixed

to

Stage

mounting

the

carrier

using screws.

Attaching/removing

rotary

mechanical

stages

(Fig. 4)

Loosen

screw on

cap2) ((3

... 4

mm).

Attach notch of stage dovetail to the spring4) atpinthe( front.

	Push stage against spring pin and lower	into	the stage
	carrier in the back, then let go.
	Tighten screw on cap2). (
The	stage can now be rotated to the right	and	left.

13

Stand

3

4

5

2

1

1	Stage	centering		screw	for	stage centration (on both sides)
2	Screw on		cap for spring pin of stage clamp
3	Stage	carrier
4	Dovetail		guiding	device
5	Clamping		lever
Fig. 5	Height		adjustment		of	the stage carrier

1

2

3

			6	5	4
1	Clamping screw for height stop,				prevents the	specimen from
	being	pressed	out	inadvertently	from below by	the condenser
2	Carrier	guidance
3	Height adjustment controls on both sides
4	Clamping screw to fix the condenser
5	Centering screws on both sides
6	Orientation groove for the condenser
Fig. 6	Condenser		carrier

14

Stage

centering

All

stages

are

factory precentered, i.e. when the stage is

rotated,

a

set

specimen feature will remain in the

image

center. Should a set feature move away from the image

center

when

the

stage

is

rotated, the

stage

must

be

centered

again.

Loosen

stage

clamping

screw

(Fig6)..

4/

Correct

the

drift of

the

image

part

by

simultaneously

rotating

the

two stage centering screws1).

(

When the

stage is centered, tighten the screw on cap.

Note:;When highly magnifying objectives are used the

centration is only exact for one chosen objective.

Height

adjustment

of

the

removable

stage

carrier

(Fig. 5)

The height

of

the

removable

stage carrier

is adjustable,

which

is

very

useful,

for example, in the case of very high specimens

(max.

specimen

height

=

49

mm).

Hold stage carrier3) ( with your left hand. Loosen clamping lever5)(.

Change	height of	stage	carrier,	tighten	clamping5).lever	(
Do not press too hard.
Note:;To remove the stage carrier, loosen					the clamping	lever
and take	the carrier	out of	the	dovetail	guiding2) deviceby	(

moving it to the left. Orientation of the clamping lever can be

selected	as	required by	pressing against the spring and
rotation	in	to the required	position.

Condenser carrier(Fig. 6)

The condenser carrier is screwed to the stage carrier. All the

condensers

available

for the Axioplan 2

imaging

are

attached

to the condenser carrier. The height

of

the

carrier

can

be

adjusted on both sides and permits

the

centering

of

the

inserted

condensers.

Note:;For

the

setting

of

KÖHLER illumination,

the

image

of

the

luminous field diaphragm must be

centered

in

the

field

of

view.

Setting the height stop(Required KÖHLER illumination

setting

must

have

been

performed):

Loosen clamping screw1) (for height

stop

using

SW

3.

Set

the

specimen.

Image the luminous field diaphragm

sharply (by adjusting

the

height

of the

condenser).

Move up the condenser by approx. half a rotation of the control.

Tighten clamping screw for height 1stop). (

Note:;Depending on which stage carrier is mounted (fixed or

removable),			the	appropriate special condenser carrier is
screwed on. Its difference mainly lies in					its height	adjustment,
and	is	of	interest	only for incident light	microscopy	and where
high	specimens in			the range of up to	49 mm are used. If you
intend		to	change	the condenser carrier, please get in touch
with	our		service	department.

B 40 042 e 09/99

Stand

3

2

1

1	Thread (normally W0.8", M27 for incident			light darkfield) to
	screw in the objectives
2	Knurled ring for nosepiece rotation
3	Compartment	for compensators
Fig. 7	Objective	nosepiece
		1		2

		Viewing
	4	window	for
		adjusting	aid
	3

1	Image of the light arc
2	Mirror		image	of	the light	arc	of the lamp reflector
3	Slide in		knob	for	adjusting	aid	activation
4	HBO 50 lamp housing
Fig. 8	Adjusting			aid

20	Objective	nosepiece
The	objective nosepiece is used for mounting the objectives
and	changing them quickly. Depending on the application,
the	customer can	choose from nine different nosepieces.

Note:;Nosepiece positions which are not being used must be covered with dust caps.

The

nosepieces for polarization is equipped with centering

screws (SW 1.5) for the centration of the objectives.

Nosepieces for

DIC

feature

one compartment for insertion of

a DIC slider in each objective mount.

Note:;Please

do

not

try to

remove the

nosepiece

from

the

stand,

since

this

will

result

in

the loss

of

the

centration;

this c

only

be reset by our service staff.

11

Compartment for the insertion of auxiliary

objects

and

compensators

Fig.

7/3

and

Fig.113/

(→ Microscope Components)

12

Reflector

turret

The

reflector

turret

(Fig12. )3/consists of a

filter wheel

with

5

click

stops

to

which

the

required

reflector

modules

are

attached. Attachment and change of modules can be per formed by the user→ Microscope( Components, Reflector Modules).

13Analyzer Compartment

Fig. 3/13

(→ Microscope Components)

14Adjusting aid for HBO/XBO incident microscope illuminators

(Fig. 8)
The adjusting			aid	simplifies	optimum setting of			the	HBO
50/100	and	XBO		75 lamps	in	fluorescence	microscopy.
A mirror, directs the image					of the light arc to a			round	wind
visible	from	the	outside (matt			black filter).	Here,	the position

change of the focal point and its mirror image, performed by

adjusting		the	screws		on	the	microscope illuminator, can				be
viewed.
The	lamp is		set	optimally		if	the	image	and the	reflected	imag
of	the	light	arc	are	shown		in	the	window	centrally	and i

focus.

15

B 40 042 e 09/99

Stand

1

8

2

7

6

5

3

4

1	Connecting tube for microscope lamps incident light
2	Ventilation		slots
3	Socket	for	instrument plug
4	Connecting tube for microscope lamps transmitted light
5	Sockets for microscope lamps transmitted light
6	Toggle switch transmitted light/incident light
7	Sockets for microscope lamps incident light
8	Socket for Axiophot 2 Photo module
Fig. 9	Back	of	manual stand, also see Fig. 2

16

Back of manual stand

Key to Fig. 9 (back of manual stand)

1Connecting tube for microscope lamps incident light with

locking screw for dovetails to fix the lamps

2	Ventilation		slots (always	keep	clear;	minimum	distance:
	15 cm)
3	Sockets for		instrument	plug	with	integrated	compart
	ment	for instrument fuses (change				of → fuseschapter
	Care,	Maintenance)

4Connecting tube for microscope lamps transmitted light,

with locking screw for dovetails to fix the lamps

5	Sockets		of	the integrated power unit for HAL 100 / trans
	mitted		light microscope			lamps
6	Toggle		switch to		change between incident and transmit
	ted	light.
	The	short		delay after you push the switch helps prevent
	you	from		hurting	your	eyes in	the unexpected bright light.
7	Sockets		of	the integrated power unit for HAL 100 incident
	light	microscope			lamps
8	Socket		for	Axiophot 2		Photo	module

B 40 042 e 09/99

Stand

9

			8	7	6	5	4 3	2	1
1	ON/OFF	switch
2	Shutter	switch incident light
3	Gear switchover to motor focusing
4	Turning to the right of the connected motorized components
5	Turning to the left of the connected motorized components
6	Coarse/fine drive			of	focusing
7	Rapid	stage	lowering		CHANGE (on both sides of the stand)
8	Rapid	stage	lowering		VIEW (on both sides of the stand)
9	Socket for connection of motorized condensers
Fig. 10	Motorized		stand

Stand	E/Stand mot
Specialities
o Integrated electronic		system	in the stand base
o Motor	focusing (only	MOT	stand)

Manual operation of these stands differs only slightly from the

operation	of the	manual	stand.	The	following	therefore
describes	only those stand functions which the instructions
for the	operation	of the	manual	stand	do not	cover.

The stand described here is equipped with all the available motorized and coded functions and may therefore differ from your stand configuration.

Note:;Microscope		Software
Some	of the motorized		functions	of the	stands can only (or
also)	be operated from the PC via the				software.
For a	description	of this	function,	please→	thsee chapter

Microscope Software.

The following numbers refer to Fig. 10 (motorized stand).

1ON/OFF switch

OInstrument switched off

IInstrument switched on

For checking the operating status,	the switch	lights	up green
in position I (for malfunctions→ seechapterCare, Mainte
nance).
When the Axioplan 2 imaging is	switched	on, not	only the

HAL 100 microscope lamp is supplied with power, but also the

Axiophot 2 Photo module, (if		it	is attached),	and the	motor
ized and coded	components.
Note:;Motorized	focusing and	all	the other	motorized	or

codable functions are only available when the instrument is switched on.

17

B 40 042 e 09/99

Stand

2

Shutter

switch

incident light

7, 8

Rapid

stage

lowering

CHANGE/VIEW

In

incident light

microscopy or when using an

externallyKnobs

on

both

sides

of

the

stand for rapid stage

lowering and

supplied

gas

discharge

lamp, it may be necessary

to blockmovingthe

it up again in the previous position.

light path without switching off

the

lamp. A

motorizedStage

lowering

shutter

is

used

for this, which interrupts the light

path

byPress

CHANGE knob7). (

swinging

in

a

diaphragm.

Stage will be lowered. The current focus setting is stored.

Normally,

the

shutter

is

opened and the lamp for

transmittedWhen

the

stage

is lowered, motorized

focusing

by

focus

light therefore switched

off.

ing

drive

is

switched

off.

A short press of switch2) blocks( the incident light and theMoving stage up

transmitted light

illuminator

is switched

on.

Press

VIEW knob8). (

If

you

want

to

combine

transmitted light

fluorescence

and

Stage

moves

up

and

the

stored focus

position

is

precisely

epi fluorescence

,

press

switch2 ( shutter)

for

more than

2

s.

reset.

The incident light or transmitted light beam path can then

be The

user friendly

design

of

this function enables

you

to

used. Pressing switch2) ( again returns to the change mode

operate

the

CHANGE/VIEW

keys with either your right

or

between

transmitted

and

reflected

light.

your

left

hand.

Note:;If you see no light in the transmitted or incident light technique, check by pressing switch2) whether( the shutter

is blocking the light path.

CAUTION!

3Gear switchover to motor focusing

Pressing the switch changes from coarse to fine drive, and vice versa (Fig. 10/3).

Risk of	injury and	instrument damage
Please do not insert your			hand or	any objects
between	the stage	and the	objective	when the

stage is moved upwards. The same applies to the downward motion: in that case, the space between condenser and stand base is reduced.

6

Motorized

focusing

drive

9

Socket

for

motorized

condensers

Focusing is manual by means

of a control knob (on

both Bothsides) the

voltage

supply

and

the

control

of motorized

con

that acts on an electronic encoder.

densers is made via this socket.

Difference:

To

change

between

coarse

and

fine

drive,

the

Switch

off

Axioplan 2

imaging

gear

switchover (Fig. 310/) must

be

operated.

Attach condenser to condenser carrier

Connect plug of the condenser cable with 9socket)on (

4, 5

Turning

to

the

left

or

right

of

the

connectedstand

base.

motorized components(Fig. 10)

Using the keys4) (and5)( attached to the right and

left of theNote:;When

the

Axioplan

2 imaging

is

switched

on,

stand you can operate two of the following

motorizedinstrument

initialization

is

performed

to allow

recognition

of

components:

the connected modules. This means that the system cannot

1.

Condenser

turret

recognize any modules which are connecafterd

the Axio

2.

Reflector

turret

plan 2

imaging has

been switched

on.

3.

Nosepiece

If

the condenser is to be

activated

via the

Microscope

control,

Key 4() turns the appropriate

control

element

of

the

compoit must

be activated

via

the

Setup

program

nent

to

the

right,

5key) to(

the

left.

(→ microscope control software).

Each time the microscope is switched on, the existence of

motorized components is "enquired" and recorded.

The

switching

function

for

the

first two

components

available

is

then assigned to keys4) and(5)(

in

the

above

sequence.

The first component found is

always

assigned

to

the

keys

on

the right of the stand. For example, if your

instrument

is

equipped

with

the

motorized

components

1

to 44,) andkeys

(

(5)

on the

right

will

switch

the

condenser

turret

and

the

keys

on

the

left

switch

the

reflector

turret.

18

B 40 042 e 09/99

Stand

7

6

	1	2		3	4	5
1	CAN bus 1 connector
2	CAN bus 2 connector
3	TV	connector (input)
4	Impedance switching for TV port
5	Connector			for Optovar / zoom intermediate tube
6	RS	232	C	interface
7	SET	programming key
Fig. 11	Back		of	motorized stand

Back of stand E/MOT

1CAN bus61 connector

For connection of the Axiophot 2 photo module.

2CAN bus62 connector

These		interfaces		permit activation of the Axioplan 2 imaging
via	a	CAN	bus.	Normally,	the	signals	from a PC which m
be	equipped		with an appropriate driver board (CAN bus inter
face)		are	transferred to		the	Axioplan	2 imaging and read o
from		there.	The	second connector also			allows cascading. This

enables several series switched instruments to be activated by a PC.

The control commands for programming languages under

WINDOWSTM		are provided			by DLynamicLs (D		inkL ibraries)L			.
			see→
For	details,	please		the	chapterInterface		Description or
get	in touch with		our service department.

5Connector for Optovar/LzoomL intermediate tube

The intermediate tube (coded or motorized) installed is con nected to the microscope via this connector using the cable supplied.

19

B 40 042 e 09/99

Stand

6

RS

232

C

interface

Coded

microscope

parts

The intermediate tube (coded or motorized) installed

is

con

nected to the microscope via this connector using

the

cableVarious

modules

of

the

Axioplan

2

imaging and

some

supplied.

additional microscope components can be coded.

Codings is beneficial when the

microscope

software

is

used.

7

Programming

key

(SET)

The software can recognize coded parts of the microscope

This key only functions in conjunction with the light

manandger

display

their

position

or

status,

even

if

the

parts

involved

described

later.

are not motorized. For example, filter wheels or intermediate

A

short

press

of

the

SET

programming

key

(<

3

s)

tubes can be coded without being motorized. In

this case,

the

stores

the

of the

software would record and display a manual change

current settings of the following microscope

parts,filter or optovar/zoom position. However, the microscope

provided

they

are

adjustable

by

motor

or

electronically

must

be appropriately

configured

if

it

is

to recognize

readable:

software

the coded

positions.

o aperture

diaphragm

for

transmitted

light

The

configuration

is

performed

with

the

aid of

a

setup

pro

o luminous

field

diaphragm

for transmitted

light

gram

delivered

with

the

instrument.

Normally,

the

setup

o incident light

aperture

diaphragm

program

has

already

been implemented

in

the factory

prior

o filter

wheels

to

delivery

of

Axioplan

2

imaging.

o condenser

front

optics

(only

with

use of achromatic motorized dual

condenNote:;

Equipping the nosepiece

ser

0.5,

445341 0000 000, the so called

pathology The

microscope

software

must

be

started

via

the

PC

before

condenser

mot.)

the

nosepiece

is

fitted

with

objectives

(normally,

the

nose

One separate storage is possible for each position of

the

nosepiece

is

fitted

with

objectives

in the

factory

as

ordered). Fit the

piece.

nosepiece mounts with the objectives displayed

for

these

The stored settings are automatically activated when

themounts by the program. If the objective to be

used

is

not

respective

positions

of

the

nosepiece

are

adopted.

displayed

by the

program,

the

setup

program

must

be

called

The stored values are retained after you switch

off

andthe

this

objective

input

for

a

mount

on

the

nosepiece.

instrument.

Always

check

whether

the

objective

position

and

the

If you have

changed

the

settings

of

the

current

position appropriateand

display

correspond

in

the

program.

The

same

now wish to call the

stored

settings,

all

you

have to

doappliesisturnfor the positions of the reflector

turret.

the nosepiece further and then return

it

to

the

previous

position.

Note:;Optovar

and

zoom

intermediate

tube

If you have inserted an Optovar intermediate tube

on

your

Axioplan

2

imaging,

its

Light

Managers

settings

(field

stop

and

filter

wheels)

are

also

stored

depending

on

the position

of the motorized objective nosepiece.

The

microscope

software

can

be

used

to

store

several

settings

for

each

mount

of the nosepiece. The settings are

stored

by

the

program

in

parameter

files

and

are

called

with

these

again.

The

microscope

software

can

be

used

to

store

several

settings

for

each

mount

of the nosepiece. The settings are

stored

by

the

program

in

parameter

files

and

are

called

with

these

again.

20

B 40 042 e 09/99

Stand

Light manager	Press	and	hold	(> 3 s)	both upper switches			for fast stag
	lowering (Fig. 12/1			left and right) at		the	same	time until
	the	orange	LED	display	(Fig2. )12/lights	up	briefly.

Light manager means the microscope's ability to adjust the

This activates the so called "learning mode" with the dry

KÖHLER illumination setting automatically. At

the

same

time

objective

mentioned

above

as

reference

system.

the

image

illumination

is adjusted

at a

constant

color

tempe

If you want to adjust the

focusing speed in the fine driv

rature.

press

both

left

switches

for

fast

lowering.

Configured settings are stored and are reproduced

automati

The upper switch causes a

coarse

focus

adjustment,

while

cally whenever you change the position of the

objective

the lower switch causes a

fine

focus

adjustment.

Holding

nosepiece.

the

switch

slowly

changes

the

focusing

speed

in

the

fine

It is

not

necessary

to

connect the

microscope

to

a computerdrive.

You

can

now

choose

among approximately

40

dif

to

perform

this

function.

ferent

focusing

speed

parameters.

Focus again exactly on the specimen and press briefly

the

Essential

requirements

for

the light manager, however, are

upper right switch. This stores focusing speed and focus

the

motorized or

coded

nosepiece

and

the

possibility

of

position

for

the

reference

objective

currently

in

use.

motorized

adjustment

for

the

following

components:

Now switch to the objective with the next lower magnifi

o luminous

field

diaphragm for

transmitted

light

cation/aperture

and

adjust

focusing

speed

and focus

posi

tion

accordingly.

You

may

store

the

parameters

for

this

o aperture

diaphragm

for

transmitted

light

objective

by

pressing

briefly

the

upper

right

switch

for

fast

o filter

wheels

lowering.

o condenser

front

optics

Proceed with the other objectives accordingly. Oil

immer

(only

with

use

of

achromatic

motorized

dual condenser

sion

objectives

should

be

adjusted

last.

0.5, 445341 0000 000)

Finally press and hold (> 3 s) the upper right switch for f

o aperture

diaphragm

for

incident

light

(only for

incident

lowering

until the

orange

LED

display

lights

up

briefly.

This

light

techniques)

switches the "learn mode" off

and

you may

now

check

The

KÖHLER

illumination

setting

procedure

should

already

the

objectives'

parfocality

visually.

have been performed prior to the inital use of your

Axioplan 2 imaging→ (page 74). You should also make an

occasional check

to

ensure

that this setting has not changed.

The factory programmed setting of the light manager

guarantees

a

practical

starting situation, from which you can

make

your

own

settings

and

then

store

them

with

the

SET

key.

Give

SET

key

(in

Fig7. )11/a

short

press

to

store

the

set

ting.

Automatic

balancing

of

focusing

speed

and

focus

position

(parfocality)

of

diffe6

rent

objectives

The lower part of the MOT

stand is equipped with a Harmonic

DriveTM

of high

precision which converts

the

hand

movement

in the focusing process into different sensitive vertical stage

movements (proportionally to

the objective's magnification).

1

2

Motor

focusing

also

compensates

minor

focus differences

1 Fast stage lowering

VIEW

which

always

occur between

the

objectives.

First,

however, 2 LED

the following

learning

process needs to

be

activated:

Fig. 12 Motorized stand

Focus dry objective exactly with the highest magnifica tion/aperture.

21

B 40 042 e 09/99

Microscope control software AxioVision Control

What does AxioVision Control offer?

AxioVision Control permits the remote control of the following Carl Zeiss microscopes from your PC:

o Axioplan	2 imaging / Axiophot 2
o Axiovert	100 M
o Axioskop	2 MOT

Remote control with AxioVision Control provides the following benefits:

o convenient remote control functionwith visual navigation aid

o flexible storage of microscope settingsand activation via

function	keys
o AxioMouse		permits microscope	operation via	PC mouse
o convenient		photomicrography with Axiophot		2 and
MC 200
o management of different user			profiles

System	requirements
Rcommended		hardware		and	software:
o Pentium	II	prozessor		300	MHz
o 128 MB	RAM
o 4 GB hard disk
o video adapter			1024	x 768		x 24	bits, 4	MB	for true color
o CD ROM drive
o 17" monitor
o Windows		98 /	Windows NT			4.0	Service	Pack	3

Handling

Minimum hardware and software requirements:

o Pentium		II	processor	233 MHz
o 32 MB		RAM
o 4 GB hard disk
o video	adapter 1024			x	768	x 24	bits,	4 MB	for true color
o CD ROM drive
o 17"	monitor
o Windows		95
Note:;Detailed			information		on	the	microscope		control
software	is	contained		in	the	AxioVision		Control manual
B 40 623	(Cat. No. 000000 1068 563)

22

B 40 042 e 09/99

Microscope control software AxioVision Control

3

1

	2
1	RS 232	C	connection
2	CAN bus	connection
3	Socket for		Axiophot
Fig. 13	Motorized		stand (back)

1

1	Serial	interface RS	232
2	Power	socket
3	2 CAN bus sockets		4 3	2
4	Spare	socket

Electrical

connections

Microscope

The coupling

of

the

Axioplan

2

with

the

PC or

the

noteboo

is

performed via

the

RS

232

C serial

interface1) (Figusing.13/

a

connecting

cable.

Axiophot

2

photo

module

The electrical

connections

are

on

the

back

of the

Axiophot

2

photo

module:

o CAN bus

socket

for

cable

connection to the stand and

other

microscope

components

(Fig. 314/)

o RS

232

serial interface

for

the connection of

a

PC

or

note

book

(Fig. 14/1)

o Spare

socket

(Fig. 14/4)

o Power

socket

(Fig. 14/2)

Connection

to

the

manual

stand

Connect

the

supplied

RS

232

C

connection

cable

to

the

serial

interface of

the photo module (Fig1) . and14/ to the

serial

interface

of

your

notebook/PC.

Connect

the internal voltage supply cable supplied to the

POWER

socket

(Fig. 14/2)

of

the photo module and to

socket

(Fig. 13/3)

at the

back

of

the

stand.

Tighten

all

the

safety

screws

on

the

plugs.

Connection

to

the

stand E/MOT

Use

the

RS

232

C cable

supplied

to

connect

the

serial

in

terface

of

the

photo

module

(Fig1). 14/or the

stand

(Fig. 13/1)

to

the

serial

interface

of your notebook/PC.

Connect

the internal voltage supply cable supplied to the

POWER

socket

(Fig. 14/2)

of

the photo module and to

socket

(Fig. 13/3)

at the

back

of

the

stand.

Connect

photo module and stand by connecting the CAN

bus

cable

to

the

appropriate

CAN

bus

sockets 3 (Fig. 14/

and

Fig. 13/2).

Tighten

all

the

safety

screws

on

the

plugs.

Fig. 14 Electrical connections of the Axiophot 2

23

B 40 042 e 09/99

System Overview

Condensers

DIC prism II / 1.4

(445375 0000 000)

DIC prism III / 1.4

(445376 0000 000)

Achromatic aplanatic universal condenser 0.9 H

(000000 1035 632)

Achromatic aplanatic universal condenser 0.9 H D Ph

(445436 0000 000)

Achromatic aplanatic universal condenser 0.9 H D Ph DIC

(445439 0000 000)

Achromatic switching condenser 0.9 H

(000000 1017 688)

Achromatic switching condenser 0.9 H D Ph

(000000 1017 690)

DIC prism I / 0.9 (with polarizer)

(000000 1005 002)

DIC prism II / 0.9 (with polarizer)

(000000 1005 003)

DIC prism III / 0.9 (with polarizer)

(000000 1005 004)

DIC prism I / 0.9

(000000 1004 901)

DIC prism II / 0.9

(000000 1004 902)

DIC prism III / 0.9

(000000 1004 903)

Front lens 0.9 / d=2.9 Pol

(445331 0000 000)

Front lens 0.6 / d=4.2 LD, a=6.8 mm

(445330 0000 000)

		Achromatic aplanatic swing in
	Achromatic aplanatic condenser 1.4 H Ph DIC,	condenser system 0.24/d=10.7
		(445325 9901 000)	Brightfield insert
	(445453 0000 000)
			(445467 0000 000)
		UV condenser 0.6 Pol for Axioplan 2 imaging Pol
		(445460 0000 000))

	Achromatic condenser 0.8 H	Achromatic condenser 0.8 H mot.
	(445443 0000 000)
		(445444 0000 000)

Achromatic condenser 0.8 H D Ph mot. Achromatic condenser 0.8 H D Ph DIC (445446 0000 000)

(445445 0000 000)

Achromatic dual	condenser 0.5	Achromatic dual	condenser 0.5 mot.
(445340 0000 000)		(445341 0000 000)

Darkfield illuminator

(445314 9901 0000 000)

Ultra condenser 1.2/1.4 (0.75 1.0)	Dry darkfield condenser
(465500 0000 000)	0.8/0.95 (0.6 0.75)
	(465505 0000 000)

Condenser holder Z

(445323 0000 000)

24

B 40 042 e 09/99

System Overview

Stage carriers, Stages, Condenser carriers

Specimen holder with spring clip R

(453533 0000 000)

Rotary, centerable mechanical stage 75x50 _/ R240

(453502 9904 000)

Mechanical stage 75x50 R with

electrical vernier scale

(453507 0000 000)

Adapter for mineralogy specimen mount

(453541 0000 000)

Specimen holder A

(000000 1046 521)

Specimen holder A

(453539 0000 000)

Specimen holder for mechanical stage 000000 1063 835/836

(000000 1046 521)

Mechanical stage 75x50 L	Mechanical stage 75x50 R
with ergo drive	with ergo drive
(000000 1063 836)	(000000 1063 835)

Rotary stage Pol

(453550 0000 000)

with object guide Pol

(453560 0000 000)

Mechanical stage 75x50 mot with control unit

(000000 1025 145)

Specimen holder with special fixation for the mounting of two specimens

(453538 0000 000)

Specimen holder D Pol

(453563 0000 000)

Specimen holder A Pol

(453564 0000 000)

	optional
	Joystick	for 2 axes	Fixed stage carrier
	(000000 1033 996)
			(452121 0000 000)
	or
	Coaxial	electronic drive
	(000000 1034 960)

Lowerable and removable stage carrier

(452122 0000 000)

Centerable condenser carrier with height adjustment on both sides

(452125 0000 000)

Attachable stage carrier ZA

(451826 0000 000)

Centerable condenser carrier with height adjustment on both sides for removable stage carrier

(452127 0000 000)

25

B 40 042 e 09/99

System Overview

Stand, Tubes, Eyepieces, Filters, Reflectors

Double video adapter

(000000 1058 640)

Binocular phototube with height adjustment

(452146 0000 000)

				Binocular	phototube	with
				sliding prism 30/25 (100/100/50:50)
				(452143 0000 000)
Eyepiece	Pl	10x/25	Br.
(444033 0000 000)
Eyepiece Pl 10x/25 Br. foc.
(444034 0000 000)
Eyepiece	Pl	16x/16	Br.
(444053 0000 000)
Eyepiece Pl 16x/16 Br. foc.
(444054 0000 000)
Eyepiece E Pl 10x/25 Br. foc.
(444234 0000 000)				Binocular	tube with
Eyepiece E Pl 10x/23 Br. foc.				two TV	ports _30/25	(100/50:50/30:70)
(444235 0000 000)				(452145 0000 000)

Reflector module H

(000000 1046 274)

Reflector module D

(000000 1046 276)

Reflector module DIC

(000000 1046 277)

Reflector module DIC red I

(000000 1046 278)

Reflector module Pol

(000000 1046 279)

Reflector module Fl

(000000 1046 281)

Optovar module 2.5x

(000000 1046 282)

Optovar module 1.6x

(000000 1046 283)

Optovar module 1.25x

(000000 1046 284)

Analyzer module D

(000000 1046 958)

Luminous field diaphragm insert D

(452160 0000 000)

Luminous field diaphragm insert D mot.

(452161 0000 000)

Double TV tubus mot.

(000000 1054 146)

Reflector module H

(000000 1046 274)

Reflector module Fl

(000000 1046 281)

Filter dia. 25 mm and beam splitter

(according to price list)

Upper stand part of Axioplan 2 imaging

(000000 1050 687)

Upper stand part of Axioplan 2 imaging mot.

(000000 1050 688)

Incident light illuminator

(452171 0000 000)

Incident light illuminator Fl

(452170 0000 000)

Filter slider A

(452174 0000 000)

Integrated 12 V/100 W power unit

(452185 0000 000)

Transmitted light illumination

(452150 0000 000)

Axioplan 2 imaging stand base

(000000 1060 417)

Axioplan 2 imaging mot. stand base

(000000 1052 299)

Axioplan 2 imaging El. stand base

(000000 1052 300)

Filter magazine D

(452155 0000 000)

Filter magazine D photo

(452157 0000 000)

Filter magazine D mot.

(452156 0000 000)

26

B 40 042 e 09/99