Zeiss Axiophot 2 User Manual

4 (1)

Axioplan 2 imaging and Axiophot 2 Universal Microscopes

Operating Manual

Knowledge of

this

manual

is required

for the operation

of the instrument. Please therefore

familiarize

yourselft with

its

conte

and

pay special regard to the sections dealing with the

safe handling of

the

instrument.

 

 

 

 

 

 

Unless

expressly authorized,

forwarding

and duplication

of this document,

utilization

and communication of its conten

are

not permitted. Violations will entail

an obligation

to

pay

compensation.

All

rights reserved in the eventatentsof granting of p

or

registration

of a

utility

model.

It is not

permitted

to

pass on or copy this

document,

or

to use

or essforward

its

conten

prior consent

has

been

provided.

If

this regulation is

not observed, damages

will be claimed.

All patentingd. rights are rese

For further details, please contact:

Carl Zeiss

Microscopy

D 07740

Jena

 

 

Phone:

++49 3641

64 16

16

Telefax:

++49 3641

64 31

44

E mail:

micro@zeiss.de

 

Internet:

www.zeiss.de/micro

Subject to change

B 40 042 e 09/99

Contents

Notes on the safe operation of the instrument. . . . . . . . . . . . . . . . . . . . . . . 3. General . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4. . . . . . . . .

Purpose . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6. . . . . . . . .

Overview and Connections. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7. . . .

Putting into Operation. . . .

. . . . . . . . . . . .

. . . .

.

. .

. . . . . . . . 8. . . .

Stand . . . . . . . . . . .

. . . . . . . . . . . .

. . . .

. .

. .

. . . . . . . . . . .10. . . . . . . . . .

Manual stand. . . . . .

. . . . . . . . . . . .

. . . .

. .

. .

. . . . . . 11. . . . . .

Stand E/Stand mot. . .

. . . . . . . . . . . . .

. . . . . . . . . . . . . . 17. . . . .

Light manager . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21. . . . .

Automatic balancing of

focusing speed and focus

position. . . .

.

. .

. . . . . . . . . . 21. .

Microscope control software AxioVision Control. . . . . . . . . . . . . . . . . . . . 22. . . .

What does AxioVision Control offer?. . . . . . . . . . . . . . . . . . . . . . . . 22. . . .

System requirements. . . . .

.

.

. . . .

. .

. . .

. .

.

.

. .

. .

. .

.

.

 

. 22. . . . . .

Electrical connections . . . .

.

.

. . . . .

. .

. . .

. .

.

.

.

. . .

. .

.

 

.

. 23. . . . .

System Overview. . . . . . . .

. . . . . . . . . . . . . . . . . . . . . . . 24. . . . . .

Microscope Components . . . . . . . . . . . . . . . . . . . . . . . . . . .

. . 31. . . . .

Microscope illuminators . . .

.

.

. . . . .

. .

. . .

. .

.

.

.

. . .

. .

.

 

.

. 32. . . . .

Tubes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36. . . . . . . .

Eyepieces . . . . .

.

. .

.

.

. . . . . .

.

.

.

.

.

. . . . . . . .

.

. .

. . .38. . . . .

Intermediate tubes .

.

. .

.

.

. . . . . .

.

.

.

.

.

. . . . . . . .

.

. .

. . 40. . . . .

Reflector

modules. .

. .

.

.

. . . . . . .

. .

.

.

.

. . . . . .

. .

. .

.

.

43. . . . . .

Objectives

. . . . .

. .

.

.

. . . . . . .

. .

.

.

.

. . . . . .

. .

. .

.

.

46. . . . . .

Stages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48. . . . . . . .

Condensers for Transmitted Light. . . . . . . . . . . . . . . . . . . . . . . . . 52. . . .

Analyzers, compensators, auxiliary objects and DIC sliders. . . . . . . . . . . . . . . . . 57. . Axiophot 2 Photo module. . . . . . . . . . . . . . . . . . . . . . . . . . . . 59. . . .

Microscopy Techniques. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63. . . . . .

Transmitted light brightfield. . . . . . . . . . . . . . . . . . . . . . . . . . . 63. . . . .

Transmitted light darkfield. . . . . . . . .

. . .

. . . .

. .

.

.

. .

. .

.

.

.

64. . . . .

Darkfield illumination in low magnifications. .

. . .

. . . .

. .

.

.

. .

. .

.

.

.

. 65. . . .

Phase contrast. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66. . . . .

DIC Differential interference contrast in transmitted. .light. . . . . . . . . . . . . . . 67. .

Transmitted light polarization Detection of birefringence. . . . . . . . . . . . . . . . 69. . .

Transmitted light polarization Determining theγ directionn of oscillation. . . . . . . . 70. . . .

Transmitted light

polarization Determining and measuring path differences. . . . . . . . . 71.

Transmitted light

polarization

 

Determining

the

optical characteristics

of. crystals. . .

. . .

72

Incident

light

brightfield. . . . . . . . . . . . . . . . . . . . . . . . . . . . 74. . . . .

Incident

light

darkfield. . .

.

. . . . . .

. .

. . . . . . . . . . .

. . . . .

. 75. . . . .

DIC Differential interference contrast in incident. .light. . . . . . . . . . . . . . . . 76. . .

Incident light polarization Detection of bireflection and reflection pleochroism. . . . . . . 77 Epi Fluorescence . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78. . . . . .

Photomicrography with the Axiophot. .2 . . . . . . . . . . . . . . . . . . . . . 79. . . .

1

B 40 042 e 09/99

Contents

Care, Maintenance. . . . . .

.

. .

. . . .

. . .

. . . . . .

. . . . . . .

.

.82. . . . . . . .

General . . . . . . . .

. .

. .

. . . .

. . .

. . . . . .

. . . . . . .

. . . . .82. . . . . . . . .

Lamp change. . . . . .

. .

.

. . . .

. . . .

. . . . . .

. . . . . . .

.

. 82. . . . . . . . .

Line supply . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .82. . . . . . . .

Changing the fuses. . . . . . . . . . . . . . . . . . . . . . . . . .

. . . . 82. . . . . . . .

Technical Data. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .83. . . . . . . . .

Interface Description. . . . . . . . . . . . . . . . . . . . . . . . . . .

. . . 84. . . . . . . .

Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .87. . . . . . . . . . . . .

List of Illustrations. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .89. . . . . . . .

Certification in accordance with ISO 9001

EC conformity declaration

2

B 40 042 e 09/99

Notes on the safe operation of the instrument

The Axioplan 2 imaging Universal Microscope has been

 

de

Specimens

 

hazardous

to

health!

 

 

 

 

 

 

 

signed

and

tested

in

compliance

with

EN

61010,

 

part

1

 

(DIN

The Axioplan 2 imaging is not equipped with any

VDE

0411)

and

IEC

1010 1

 

and

left

the

factory

 

in

a

 

perfect

special

devices

for

 

protection

against

corrosive,

 

state

with

regard

to

its safety facilities. To ensure

 

that

 

 

WARNING!

toxic,

radioactive

or

 

other

substances

hazardous

 

 

 

this re

 

 

mains the case and to guarantee the safe operation of the

to

health.

 

All legal requirements, especially na

equipment, the instructions and warnings given in

 

this

tional

 

accident

regulations,

 

must

 

be

observed

 

manual

must

be

observed.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

when

handling such

 

specimens.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

The instrument meets the requirements of the EC directive

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

89/336/EEC

and

the

EMC

legislation

of

November

 

9th 1992.

Hot

surfaces!

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

It has been allocated the protection degree IP 20 and

 

is

cate

Do not touch the hot lamp housing. Always dis

gorized

as

Class

1

Equipment.

The

power

plug

must

be

in

connect

the

power

plug

before

changing

a

 

lamp

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

WARNING!

and allow the unit to cool down for approx. 15

serted in a socket featuring a grounding (earth) contact. The

grounding

effect

must

not

be

nullified

by

an

extension

 

cable

minutes.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

which

does

not

have

a

protective

ground

wire.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Thermally

sensitive

 

fluorescence

filters!

 

 

 

 

For

your

own

safety

and

 

for the

protection

 

of

the

 

Fluorescence

filters

are

sensitive

to

the

 

thermal

 

 

 

radiation

of

the

microscope

lamp

and

their

perfor

instrument

against

damage

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

WARNING!

mance can be permanently impaired by it. There

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Axioplan 2 imaging features special protective devices such as

fore, never remove the heat reflecting filter on the

attenuation

filters

to

protect the eyes against intense

 

radi

microscope

 

illuminators

when

you

 

are

working

 

ation, and stops to protect specimens and objectives against

with

fluorescence

filters.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

knocks and mechanical damage. These protective

 

devices

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

must be used and must not be removed. You must familiarize

Heat

build6up!

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

yourself with the protective devices provided by Axioplan 2

Placing

objects

against

or

 

covering

ventilation

 

imaging under

all

circumstances.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

slots

on

the

microscope

or

its

 

components

can

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

WARNING!

lead

to

a

build up

of

heat

which

will

damage

the

 

 

 

Danger

of

crushing!

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

instrument

and,

in

extreme

 

cases,

cause

 

a

fire.

 

 

 

 

In

stands

with

a

motorized

focusing

drive,

there

is

 

 

 

 

 

 

Therefore,

 

always

keep

the

 

ventilation

slots

clear

 

 

 

a

danger

of

crushing

your

 

hand

between

 

the

 

 

 

 

 

 

 

(minimum

distance

15

cm).

 

 

 

 

 

 

 

 

 

 

 

 

WARNING!

stage

carrier

and

 

stand

base

 

when

the

 

stage

is

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Always

check

whether

the

microscope

is

switched

 

 

 

lowered.

For

this

reason,

do

not

place

your

hand

 

 

 

off

before

placing

the

dust

cover

 

over

it.

 

 

 

 

 

 

under

stage

carrier

 

when

the

 

stage

is

 

being

 

low

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

ered.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Dust

und

 

dirt!

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Dust

and

 

dirt

can

impair

the

performance

of

the

 

 

 

Gas

discharge

lamps!

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Axioplan 2 imaging. Therefore, protect the micro

 

 

 

In

unfavorable

circumstances

and

with

improper WARNING!

 

 

 

scope

as

far

as

possible

against

 

these

influences.

 

 

 

use,

gas

discharge

 

lamps

can

explode,

 

flinging

 

Always

use

 

the

dust

cover

if

you

 

do

not

intend to

CAUTION!

splinters

of

glass

through

the

air

and

causing

 

pos

 

 

 

use

the

Axioplan

2

 

imaging

for

longer

periods

of

 

 

 

sible

 

injury.

Therefore,

it

 

is

 

imperative

 

that

 

the

 

 

 

 

 

 

 

 

 

time

(more

 

than

6

 

hours).

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

safety and operating instructions of the manufac

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

turer

 

of

the gas

 

discharge

 

lamp

be

followed→

 

(

 

Operation!

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

chapter

onCare,

Maintenance),

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

The

instrument

must

 

be

operated

by

trained

staff

 

 

 

Gas

discharge

lamps

emit

 

ultraviolet

radiation

 

 

 

 

 

 

 

only.

They

 

must

be

 

instructed

in

 

the

 

hazards

in

 

 

 

which

can

cause

burns

on

the

 

eyes

and

 

skin. Never

 

 

 

 

 

 

 

 

 

volved

in

 

microscopy

and

the

respective

field

of

 

 

 

look

 

directly

into

the

light

of

these

 

lamps

 

 

WARNING!

 

 

 

 

 

 

 

and

application.

 

This

includes

an

awareness

of

the

risk

 

 

 

avoid

direct,

unprotected

incidence of

their

light

 

 

 

 

 

 

of

eye

injury

due

to

intensive

irradiation

by

light.

 

 

 

on your skin. When using the

microscope,

always

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

use the protective devices belonging to the instru

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

ment,

e.g.

special

attenuation

 

filters.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Gas

discharge

lamps

are

contained,

for

example,

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

in our microscope illuminators HBO 50,

HBO

100

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

and

XBO

75.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

3

B 40 042 e 09/99

General

Note on

exchangeable

components

 

 

 

 

 

 

Note

on power

unit

integrated in

stand

Perfect functioning of the instrument

requires that

you

useThe integrated power unit is used to supply voltage to the

spare parts and components which

are

marketed

and

apmicroscope

illumination, the Axiophot 2 Photo module and

proved by us. In the event of doubt,

please contact our servicethecoded

motorized components. The power unit must not

staff.

The

use

of parts

from

other manufacturers

may

impairbeused

to

supply

voltage to other external power consuming

the performance of the Axioplan

2

imaging

or

indeeddevices.

This can

lead

to overloading

and destruction of the

damage the instrument. The use of

such

parts

 

is

the powersole

unit.

 

 

 

 

responsibility

of

the user.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

No

other

care,

maintenance

or repair work

must

 

be

carried

 

 

 

 

 

 

out apart from the activities specified→ inCare,

 

 

 

 

 

 

 

 

Maintenance.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

More extensive repairs may only be performed

by

our

 

 

 

 

 

 

customer service experts or by specially authorized persons.

 

 

 

 

 

 

Damage to the instrument may otherwise result.

 

 

 

 

 

 

 

 

 

 

Note:;We would like to expressly emphasize here that any

 

 

 

 

 

 

adjustments

 

not

described

in

Microscopethe

Components

 

 

 

 

 

 

 

 

chapter must only be performed

by

persons

 

expressly

 

 

 

 

 

 

authorized by us to do

so.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

The

Axioplan

2

imaging

is

a

precise

optical

instrument

which

 

 

 

 

 

 

may be impaired in its function or even damaged by inexpert handling.

4

B 40 042 e 09/99

General

The Axioplan 2 imaging is a universal microscope

whichAbbreviationsis

 

 

 

 

 

 

 

 

suitable for all applications relevant to light

microscopy. achrIts.

achromatic

 

 

 

 

 

modular design, numerous components and wide range ofAL

incident

light

 

 

 

 

accessories allow the Axioplan 2 imaging to be adapted aplnd.

aplanatic

 

 

 

 

 

 

extended

to

 

perform

a

 

large number

of

special

applicationsBr.

suitable

for eyeglass wearers (eyeglasses can be

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

placed

directly

against

eyepieces)

There is

practically

no

field

of

application

in

light

microscopyC

camera

 

 

 

 

 

 

 

for which the Axioplan 2 imaging cannot be

used.

However,CMount

camera

mount

 

 

 

 

users wishing to utilize the motorized and automaticD

diameter

or

coverglass

thickness

functions of Axioplan 2 imaging must take this

into

accountD

darkfield

 

 

 

 

 

 

when choosing the basic stand configuration. You

willDIC

Differential

interference

contrast

already have made this decision before starting to read

DLthis

transmitted

light

 

 

 

manual. But we would nevertheless like to point out thatE Plthe

flat field eyepieces with aspheric correction

configuration

 

for

which

you

have

opted must

not

necessarilyWD

working

distance

 

 

 

be the definitive one. If you wish to change your Flbasic

fluorescence

 

 

 

 

 

configuration

 

at

a

later

date,

the

possibility

most

certainlyfo

focusable diopter compensation on eyepiece

exists. Our specialist staff will

be

pleased

to provide

you fotwith.

photographic

 

 

 

 

any

advice

you

may

need.

 

 

 

 

 

 

 

 

 

 

 

FT

chromatic

beam

splitter

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

HAL

halogen

illuminator

or

lamp

The

aim

of

this manual is to describe the

many

possibleHBO

mercury pressure short arc lamp

functions

of

 

Axioplan 2 imaging. However, the

sheer

numberHD

bright/darkfield

 

 

 

 

of functions

 

offered may sometimes lead to a

certain

amountH

brightfield

 

 

 

 

 

of

confusion.

Therefore,

if you have any doubts

concerningICS

Infinity Colour corrected System

the

capabilities

of

your

Axioplan

2

imaging,

please

contactKorr

morrection

mount

 

 

our subsidiary or ourselves direct. Our address and telephoneLD

long

working

distance

 

number

are

 

given

on

 

the

inside

cover

of

this manual.

 

LFB

luminous

field

diaphragm

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

MC

microscope

camera

 

 

In the chapter entitledStand you will find the

various

basicMPM

microscope

photometer

 

versions of the Axioplan 2 imaging stands. Starting with Nthe.A.

numerical

aperture

 

 

manual version, this chapter deals with all stand functionsP

photometry

 

 

 

 

 

available, up to and including the stand featuring

Phall 1, 2,

3 phase

contrast;

the

numbers refer to the diameter

motorizable

functions

and the

light

manager.

 

 

 

 

 

 

of the ring stop used; with an objective with the

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

designation Ph 2, you use the ring stop with the

The

chapter Microscope

Components

describes

 

the

 

 

corresponding

designation Ph 2.

operating functions with which you yourself

can

equip

Polr

polarization

 

 

 

 

 

extend the Axioplan 2 imaging. Not all parts

described

 

thereSFZ

field

of

view

number

 

must be present on the

Axioplan

2

imaging.

However,

ifSIyou

system

integration

 

 

are considering adding some of the components to yourSLR

single

 

lens

reflex

 

 

 

configuration,

the

corresponding

catalog

numbers

are

listed.Stemi

stereomicroscope

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

SW

wrench

size

 

 

 

 

 

The chapterMicroscopy Techniques provides you with in UD

universal

rotary

stage

 

formation on the basic settings of the microscope,

 

eVar.g.

VAREL

contrast

 

 

 

KÖHLER illumination adjustment, and contains

instructions vis.

visual

 

 

 

 

 

 

 

 

concerning the operation of the microscope for specific apW Pl

wide

angle

flatfield

 

plications.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

XBO

xenon

 

short arc

lamp

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

ZBE

intermediate

image

plane

5

B 40 042 e 09/99

Purpose

The Axioplan 2 imaging has been designed as a universalInstallationmi

conditions

 

croscope

and can

be

used

for

all

areas of light

microscopy,

pro

 

 

 

 

vided

it

is

configured

and

 

 

 

 

 

o

Dust free environment

 

equipped appropriately. Depending

 

relative

air humidity

85 %

on

its

equipment,

it

is

used

in

the

following

fields:

o Maximum

o Vibration free

worktop

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

o transmitted

light

microscopy

 

 

 

 

 

 

 

 

 

 

o incident

light

microscopy

 

 

 

 

 

Specifications concerning power supply, storage temperature

o fluorescence

microscopy

 

 

 

 

 

and other

technical

details are

contained in the chapter

o photomicrography

 

 

 

 

 

 

 

entitled Technical

Data.

 

 

o videomicroscopy.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

The Axioplan 2 imaging also

performs the contrasting meth

 

 

 

 

ods

in

transmitted

light

and

incident

light:

 

 

 

 

 

 

 

odarkfield

ophase contrast

opolarization contrast

odifferential interference contrast.

Motorizable functions

and

the

recognition of coded micro

scope

components

used

permits the performace of applica

tions in such fields as

process

automation

and telepathology.

Here,

the

microscope

is

controlled by a software which acti

vates

the

programmable

CAN BUS and RS 232 interfaces.

Here,

the

microscope

is

controlled by a built in microprocessor

and a

control

software

which

activates

the

standard interfaces

CAN BUS

and

RS

232

C

in

personal

computers.

6

B 40 042 e 09/99

Overview and Connections

The stand

shown in the illustration is equipped with a wide

variety of

functional units. It may therefore differ from the

stand you have purchased. Should you therefore see any

components in the drawing which are

not

present

on

your

own microscope, this does not

mean that you have an

incorrect Axioplan 2 imaging configuration.

 

 

 

 

Setting and

adjustment

of certain

components

and

modules

of the Axioplan 2 imaging sometimes

require special

tools

which are included in the delivery

package

of

the microscope.

This

particularly concerns

the

SW

3 ball headed screwdriver

and

the SW

1.5 screwdriver

for

Allen

head

screws.

 

7

8

 

6

5

4

9 3

10 2

1

1

Stand (base) with complete transmitted light illumination

2

Condenser

with carrier

3

Stage

with

carrier

 

4

Objective

nosepiece

 

5

Reflector

turret

 

6

Tube

 

 

 

 

7

Stand

(upper

part)

with complete incident light illumination

8

Lamp

for

incident

light

9

Lamp

for

transmitted light

10

ON/OFF switch

 

Fig. 1 Axioplan 2 imaging (motorized);

7

B 40 042 e 09/99

Putting into Operation

Unpacking and installing the microscope

 

 

Normally,

your

microscope is supplied completely assembled

 

in

a specially

designed container. The Axiophot 2

Photo

module and the notebook required for its control,

plus

any

required intermediate tubes, are packed separately.

The

 

transport containers include instructions for unpacking the

 

instrument.

 

 

 

Please make sure that you observe the instructions contained

therein.

 

 

 

 

Due

to

the complexity of the Axioplan 2 imaging

and

the

necessity to ensure its perfect functioning, it will generally be

installed and

set

up on

site by our

service personnel.

The

Axioplan

2

imaging

is handed over to you in

a state which

will

enable

the

users to attach

all the items listed in the

Microscope

Components chapter

themselves.

The

procedures required are

described.

 

 

 

8

B 40 042 e 09/99

Putting into Operation

 

 

 

 

 

 

 

 

 

 

Setting

up

the

microscope

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

The

Axioplan

2

imaging

microscope

 

features

an

integrated

 

 

 

 

 

 

 

 

 

 

 

wide range power unit and can be connected to line voltages

 

 

 

 

 

 

 

 

 

 

ranging from 100...240 V AC, 50

... 60 Hz. The wide range

 

 

 

 

 

 

 

 

 

 

power unit sets itself automatically

to the

appropriate

line

 

 

 

 

 

 

 

 

 

 

voltage.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Check

 

that all connected components have been

 

 

 

 

 

 

 

 

 

 

 

 

mounted

correctly and

are

sitting

properly.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Set

ON/OFF

 

switch (Fig10. 1/)

to

positionO.

 

 

 

 

 

 

 

4

 

 

 

 

 

 

 

 

 

 

Connect

power

unit

first

to

the

instrument

and

then

to

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

the

line.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

3

 

 

 

 

 

 

 

 

 

 

Make

the

other

electrical

connections,

such

as

micro

 

 

 

 

 

 

 

 

 

 

 

scope

illuminators,

connecting

cable

to

TV

camera,

 

 

 

 

 

 

 

 

 

 

 

 

 

2

 

 

 

 

 

 

 

 

 

 

Axiophot

2,

notebook,

etc.

 

 

 

 

 

 

 

 

 

 

1

 

 

 

 

 

 

 

5

 

Note:;All

the

electrical

and electronic

connections

must

be

 

 

 

 

 

 

 

 

 

6

 

 

 

 

 

 

 

 

 

 

 

made before switching on the instrument so that they can be

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

recognized

by

the initialization

routines.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Set

ON/OFF

switch to positionI.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

This last setting switches on

the

microscope

and

makes

it

 

 

 

 

 

 

 

 

 

 

ready to

use

after

approx.

10

s.

 

When

the

instrument

is

 

 

 

 

 

 

 

 

 

 

equipped

with

the light manager, this last position is set.

 

1 Sockets

of

the

integrated

power

unit

for

the

transmitted light

Switch

on

notebook

or

PC (if connected).

 

 

 

 

 

illumination

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

2 Toggle

switch

to

change between transmitted and incident light

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

3 Sockets

of

 

the

integrated

power

unit

for

the

incident light

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

illumination

 

 

 

 

 

 

 

 

Switching

 

off

the

instrument

 

 

 

 

 

 

 

 

 

 

4 Socket

for

Axiophot

2 Photo module

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

5 Socket

for

instrument

plug

(line voltage)

 

 

 

Set

ON/OFF

switch

to

positionO.

 

 

 

 

 

 

 

 

 

 

6 Compartment

for

instrument

fuses

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Fig. 2 Axioplan

2

imaging (instrument

back);

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

9

B 40 042 e 09/99

The components of the lower stand part are available in the following versions:
o condenser turret: manual or motorized o luminous field diaphragm: manual or motorized o filter wheels: manual,
coded or motorized o z focusing drive: manual or motorized
10

Stand

All the stand configurations described

in the following areFeatures and components

common

to

reflected and

trans

usually tailored to the customers special requirements. Formitted light

stand

types:

 

 

 

 

 

 

example,

 

it

is

possible that

the

stand

configuration

delivered

connection

 

 

 

 

 

 

 

to

you

includes

only

incident light or

fluorescence

 

 

o lamp

 

 

 

 

 

 

 

equipment

exit

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

o light

 

 

 

 

 

 

 

 

 

and that transmitted light components

are

therefore

noto power units

for

HAL

100

microscope

illuminator

and

available.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

sevenelectronic

control

system

 

 

 

 

 

The

same applies to

the

objective

nosepiece,

of

which

 

components

 

models

are

available.

 

 

 

 

 

 

 

 

 

 

o switches,

adjusting

and

display

 

 

 

 

 

 

 

 

 

 

 

o mechanical parts for stage focusing

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

The

following

stand

types

exist

for

the

Axioplan

2

imaging:

 

 

 

 

of stands starts with the manual

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

The following description

o Manual

type

 

 

 

 

 

 

 

 

 

 

 

model.

On

this

basis, only the

differences

and additional

 

This stand can only be operated manually. Motorized andfunctions are described for

the

other

stand

models.

 

 

codable

functions

cannot

be

easily retrofitted.

 

 

 

 

 

 

 

 

 

 

 

 

 

o Type

 

E

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Unlike the manual type, this stand is equipped with an

 

 

 

 

 

 

 

 

 

 

 

electronic

 

system

permitting all the appropriately pre

 

 

 

 

 

 

 

 

 

 

 

pared

microscope

components (except the z focusing

 

 

 

 

 

 

 

 

 

 

 

drive) to be motorized. These functions can also be easily

 

 

 

 

 

 

 

 

 

 

 

retrofitted.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

o Motorfocus

type

(MOT)

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Like type E, plus motorized focusing equipment

 

 

 

 

 

 

 

 

 

 

 

 

Aside from these basic types we also use the following

 

 

 

 

 

 

 

 

 

 

abbreviations

in

this

manual:

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

o LM/E

 

type

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Like

 

type

E,

plus

additional

light

manager

 

 

 

 

 

 

 

 

 

 

 

 

 

o LM/MOT

type

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Like Motorfocus type, plus additional light manager

 

 

 

 

 

 

 

 

 

 

 

Upgrading to include motorized functions is possible,

but

 

 

 

 

 

 

 

 

 

 

requires the stand type E or higher.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Should you intend to upgrade your microscope to include

 

 

 

 

 

 

 

 

 

 

motorized functions, please get in touch with the Zeiss service

 

 

 

 

 

 

 

 

 

 

department. Retrofitting is normally possible without

 

 

 

 

 

 

 

 

 

 

difficulty.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

The components of the upper stand part are available in the

 

 

 

 

 

 

 

 

 

 

following

versions:

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

o reflector

turret:

 

 

manual

or

motorized

 

 

 

 

 

 

 

 

 

 

 

 

 

o nosepiece:

 

 

 

 

manual,

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

coded

or

motorized

 

 

 

 

 

 

 

 

 

 

 

 

 

o reflected light

shutter:

 

motorized

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

B 40 042 e 09/99

Stand

13 14 15 16 1718

19

12

20

 

 

21

11

22

 

10

 

9

8 7

6

5 4

3

2 1

1

ON/OFF

switch

 

 

 

2

Light

intensity

control

3

On/Off display of color temperature key

4

Color

temperature

key

5

LED

line

 

lamp

voltage

6

Focusing

 

drive

 

 

 

7

Filter

wheel

2

 

 

 

8

Filter

wheel

1

 

 

 

9

Luminous field diaphragm (transmitted light)

10

Light exit of transmitted light equipment

11

Compartment

 

for

compensators

12

Reflector

 

turret

 

 

13

Analyzer

 

compartment

14

Centering screws for luminous field diaphragm (incident light)

15

Pushrod

for

luminous field diaphragm (incident light)

16

Slot

for

 

filter

slider

17

Centering screws for aperture diaphragm (incident light)

18

Pushrod for aperture diaphragm (incident light)

19

Adjustment aid for lamp setting (incident light option)

20

Objective

 

nosepiece

21

Lowerable

stage

carrier

22

Swinging in/out of diffusion disk (transmitted light)

 

 

 

 

 

Fig. 3

Manual

stand

 

 

Manual

stand

 

 

 

 

 

The

stand shown here may differ from your stand model.

Most

differences will

be present in the upper part of

the stand

i.e.

they concern incident light and polarizing microscopy. If

the

configuration

illustrated

is different

from your

own Axio

plan

2 imaging,

this

does

not necessarily mean

that

an error

has

been

made,

but

is probably due to the stand

equipment

you

have

chosen

for

your

respective

application.

 

 

Controls

(The numbers refer to Fig. 3.)

1ON/OFF switch

Position O =

instrument

switched

off

 

 

 

Position I

=

instrument

switched

on

 

 

 

For optical status checking, the

switch lights up in green in

position I

(for defects

chaptersee

Care, Maintenance).

 

 

When the Axioplan 2 imaging

is switched

on,

not

only the

HAL 100

microscope

lamp is

supplied, but

also

the

Axiophot

2 Photo module, if mounted, and the motorized internal components.

2Light intensity control

Knob to adjust the light intensity of connected 100 HAL

microscope

illuminators.

 

 

 

 

 

The

power

unit

integrated

in

the

stand

is highly

stabilized

against

voltage

fluctuations

and supplies

adjustable

d.c.

voltage

in

the range

from 3 to

12

V. A

yellow LED

line disp

the

set

voltage

range

and

the

light

intensity.

 

Note:;If the light intensity cannot be adjusted, please check whether the color temperature 4key) is( switched off.

3On/Off display of color temperature key

Lights up

when the color temperature of 3200 K has been

switched

on.

4Color temperature key

Knob to set

the

3200 K

color temperature for photomicro

graphy

using

color

films (artificial light). When switched on,

a green

LED

lights

above

the knob.

A constant color temperature of 3200 K is required for color

photography.

This is

achieved if a fixed d.c. voltage

of 10.5

is supplied

to

the

HAL 100

microscope

illuminator.

Correct

functioning

can

not

be guaranteed if other lamps than the

12 V/100 W

lamps

supplied by

Carl Zeiss

are used.

 

11

B 40 042 e 09/99

Stand

7, 8

Filter

wheels

for

transmitted

light

 

 

 

 

 

 

9

Luminous6field

diaphragm

 

 

 

 

 

 

Two

rotatable

filter

wheels

(filter

magazine)

with

4

 

 

 

 

 

 

 

 

 

positions

 

 

 

 

 

 

 

 

 

 

 

 

 

 

each

are equipped

with

different

filters;

two

different

 

 

Wheel

for

the

continuous

setting

of

the

aperture

of

the

models

 

 

 

 

 

 

 

 

 

 

 

 

 

 

are

available.

The

 

wheels,

which

feature

click

 

stops,

 

luminous field diaphragm (transmitted light).

 

 

 

 

 

 

 

are

 

 

 

 

 

 

 

 

 

 

 

 

 

 

rotated

into

position.

The

set filters are

marked on

 

the

wheels.

Light exit of transmitted6light equipment

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

10

 

 

o Filter magazine D 452155600006000

 

 

 

 

 

 

 

 

Light

filters

with

dia.

32

mm

can

be

placed

on

the

plane

Filter magazine for general use.

 

 

 

 

 

 

 

 

 

 

surface

of

the

centering

ring supplied.

 

 

 

 

 

The

positions

of

 

the

two

wheels

can

be

combined

as

 

 

 

 

 

 

 

 

 

 

 

 

 

 

required.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Note:;Additional

filters

can

be

inserted

in

the

color glass

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

carrier

of the

condenser

mount.

 

 

 

 

 

 

 

Filter

wheel

27) (100 open

position

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

25

neutral density

filter

 

0.25

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

6

 

neutral density filter 0.06

 

 

 

 

 

14

Centering

screws

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

1.5

neutral density

filter

 

0.015

 

 

 

 

 

To center the luminous field

diaphragms (incident

light)

using

Filter

wheel

18) (100 open

position

 

 

 

 

 

 

 

 

 

 

SW 3

screws.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

6

 

neutral density filter 0.06

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

G

 

wide band interference filter

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

green

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

CB

conversion

filter

3200

K ...

5500

K

 

 

 

 

 

 

 

 

 

 

 

 

 

 

o Filter magazine D PHOTO 452157600006000

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Filter

magazine

for

the

brightness

control

at

a

constant

 

 

 

 

 

 

 

 

 

 

 

 

 

 

color

temperature

of 3200 K. The 3200 K color

tempera

 

 

 

 

 

 

 

 

 

 

 

 

 

 

ture must be selected. Green LED above the key lights

 

 

 

 

 

 

 

 

 

 

 

 

 

 

constantly. The positions of the two filter wheels can be

 

 

 

 

 

 

 

 

 

 

 

 

 

 

combined

as

required.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

A high current and a correspondingly bright

 

lamp

 

are

 

 

 

 

 

 

 

 

 

 

 

 

 

 

needed to achieve the color temperature of 3200 K. Filter

 

 

 

 

 

 

 

 

 

 

 

 

 

 

wheel 2

can

be

used

to reduce

the

brightness

 

of

the

lamp

 

 

 

 

 

 

 

 

 

 

 

 

 

 

to a "normal degree" more beneficial

to

the

eye.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

The

following

brightness

steps

can

be

set:

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Filter

wheel

27) ( coarse

steps

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

100 maximum

 

brightness

at

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

3200

K

100

%

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

6

 

normal

brightness

at

3200

 

K

6

%

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

0.4 low

brightness

at

3200 K

 

0.4

%

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

0transmitted light blocked

Filter wheel 18) (fine steps

 

 

 

 

 

100 brightness

100

%

of

coarse

step

50

brightness

50

%

of

coarse

step

25

brightness

25

%

of

coarse

step

12

brightness

12

%

of

coarse

step

Conversion or

green filters are additionally inserted

into

the filter mount or laid onto the light exit

of the

transmitted light

illumination.

 

12

B 40 042 e 09/99

B 40 042 e 09/99
Fig. 4

Stand

5

4

3

2

1

15 Pushrod

For the continuous diameter setting of the luminous field diaphragm (incident light)

Pushed in = open diaphragm Pulled out = closed diaphragm.

6Manual focusing drive

The universal microscope Axioplan 2 imaging is focused

via

coaxial drives on both sides of the stand.

 

Coarse drive (large knob on the

inside):

 

1 revolution = approx. 2

mm, 1 increment = mapproxm. . 2

 

Fine

drive (small

knob

at

the

outside):

 

Gear

ratio 1:10

(1 revolution

approx.

0.2 mm)

 

22

Diffusion

disk

(transmitted light)

 

 

 

Setting

via

right/left

rotation using

 

 

 

SW 3 ball headed screwdriver

 

 

 

Diffusion

disk

OFF

 

 

 

 

Diffusion

disk

ON

 

 

The

diffusion disk

improves

the

homogeneous illumination

of

the object plane. Normally, it is swung into position. It can b

swung

out using

the SW 3 ball headed screwdriver to

increase

the visibility of the lamp filaments during adjustment

of the

microscope

illuminator.

1

Stage centering screw

for

stage

centration (on

both

sides)

21

Stage carrier

 

 

 

 

 

 

 

2

Screw on cap for spring pin of

stage clamp

 

 

 

 

 

 

 

 

 

 

 

 

specimen stages The

stage

carrier

is

used

to mount the stage and the con

3

Angular

guidance for

attachment

of dovetail

for

4

Spring pin of stage clamp

 

 

 

 

 

 

denser carrier to which the

condenser

is

attached.

The stage

5

Drilled

holes to screw

on

fixed

stages and

scanning

stages

carrier is either fixed to the stand

or

removable.

The

non

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

rotating mechanical

stage and the

scanning stage are fixed

to

 

Stage

mounting

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

the

carrier

using screws.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Attaching/removing

rotary

mechanical

stages

 

 

 

 

 

 

 

 

 

 

 

 

(Fig. 4)

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Loosen

screw on

cap2) ((3

... 4

mm).

 

 

 

Attach notch of stage dovetail to the spring4) atpinthe( front.

 

Push stage against spring pin and lower

into

the stage

 

carrier in the back, then let go.

 

 

 

Tighten screw on cap2). (

 

 

The

stage can now be rotated to the right

and

left.

13

Stand

3

4

5

2

1

1

Stage

centering

screw

for

stage centration (on both sides)

2

Screw on

cap for spring pin of stage clamp

3

Stage

carrier

 

 

 

4

Dovetail

guiding

device

 

 

5

Clamping

lever

 

 

 

 

 

 

 

 

Fig. 5

Height

adjustment

of

the stage carrier

1

2

3

 

 

 

6

5

4

 

 

1

Clamping screw for height stop,

prevents the

specimen from

 

being

pressed

out

inadvertently

from below by

the condenser

2

Carrier

guidance

 

 

 

 

3

Height adjustment controls on both sides

 

 

4

Clamping screw to fix the condenser

 

 

5

Centering screws on both sides

 

 

 

6

Orientation groove for the condenser

 

 

 

 

 

 

 

 

Fig. 6

Condenser

carrier

 

 

 

14

Stage

 

centering

 

 

 

 

 

 

 

 

 

 

All

stages

are

 

factory precentered, i.e. when the stage is

rotated,

a

set

 

specimen feature will remain in the

image

center. Should a set feature move away from the image

center

when

the

stage

is

rotated, the

stage

must

be

centered

again.

 

Loosen

stage

clamping

screw

(Fig6)..

4/

 

 

 

 

 

Correct

the

 

drift of

the

image

part

by

simultaneously

 

rotating

the

 

two stage centering screws1).

(

 

 

 

When the

stage is centered, tighten the screw on cap.

Note:;When highly magnifying objectives are used the

centration is only exact for one chosen objective.

 

Height

adjustment

of

the

removable

stage

carrier

 

(Fig. 5)

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

The height

of

the

removable

stage carrier

is adjustable,

which

is

very

 

useful,

for example, in the case of very high specimens

(max.

specimen

 

height

=

49

mm).

 

 

 

 

 

Hold stage carrier3) ( with your left hand. Loosen clamping lever5)(.

Change

height of

stage

carrier,

tighten

clamping5).lever

(

Do not press too hard.

 

 

 

 

Note:;To remove the stage carrier, loosen

the clamping

lever

and take

the carrier

out of

the

dovetail

guiding2) deviceby

(

moving it to the left. Orientation of the clamping lever can be

selected

as

required by

pressing against the spring and

rotation

in

to the required

position.

Condenser carrier(Fig. 6)

The condenser carrier is screwed to the stage carrier. All the

condensers

available

for the Axioplan 2

imaging

are

attached

to the condenser carrier. The height

of

the

carrier

can

be

adjusted on both sides and permits

the

centering

of

the

inserted

condensers.

 

 

 

 

 

 

 

 

 

 

Note:;For

the

setting

of

KÖHLER illumination,

the

image

of

the

luminous field diaphragm must be

centered

in

the

field

of

view.

 

 

 

 

 

 

 

 

 

 

 

 

Setting the height stop(Required KÖHLER illumination

 

 

setting

must

have

been

performed):

 

 

 

 

 

 

 

 

Loosen clamping screw1) (for height

stop

using

SW

3.

 

 

Set

the

specimen.

 

 

 

 

 

 

 

 

 

 

Image the luminous field diaphragm

sharply (by adjusting

 

 

the

height

of the

condenser).

 

 

 

 

 

 

 

Move up the condenser by approx. half a rotation of the control.

Tighten clamping screw for height 1stop). (

Note:;Depending on which stage carrier is mounted (fixed or

removable),

the

appropriate special condenser carrier is

screwed on. Its difference mainly lies in

its height

adjustment,

and

is

of

interest

only for incident light

microscopy

and where

high

specimens in

the range of up to

49 mm are used. If you

intend

to

change

the condenser carrier, please get in touch

with

our

service

department.

 

 

B 40 042 e 09/99

Stand

3

2

1

1

Thread (normally W0.8", M27 for incident

light darkfield) to

 

screw in the objectives

 

 

 

2

Knurled ring for nosepiece rotation

 

 

 

3

Compartment

for compensators

 

 

 

 

 

 

 

 

 

 

Fig. 7

Objective

nosepiece

 

 

 

 

 

1

 

2

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Viewing

 

4

window

for

adjusting

aid

 

3

 

 

 

 

 

 

 

 

 

 

 

1

Image of the light arc

 

 

 

2

Mirror

image

of

the light

arc

of the lamp reflector

3

Slide in

knob

for

adjusting

aid

activation

4

HBO 50 lamp housing

 

 

 

Fig. 8

Adjusting

aid

 

 

 

 

20

Objective

nosepiece

The

objective nosepiece is used for mounting the objectives

and

changing them quickly. Depending on the application,

the

customer can

choose from nine different nosepieces.

Note:;Nosepiece positions which are not being used must be covered with dust caps.

The

nosepieces for polarization is equipped with centering

screws (SW 1.5) for the centration of the objectives.

 

Nosepieces for

DIC

feature

one compartment for insertion of

a DIC slider in each objective mount.

 

 

 

 

 

Note:;Please

do

not

try to

remove the

nosepiece

from

the

stand,

since

this

will

result

in

the loss

of

the

centration;

this c

only

be reset by our service staff.

 

 

 

 

 

11

 

Compartment for the insertion of auxiliary

 

 

 

objects

and

compensators

 

 

 

 

 

Fig.

7/3

and

Fig.113/

 

 

 

 

 

 

 

 

 

(→ Microscope Components)

 

 

 

 

 

 

 

12

 

Reflector

turret

 

 

 

 

 

 

 

The

reflector

turret

(Fig12. )3/consists of a

filter wheel

with

5

click

stops

to

 

which

the

 

required

reflector

modules

are

attached. Attachment and change of modules can be per formed by the user→ Microscope( Components, Reflector Modules).

13Analyzer Compartment

Fig. 3/13

(→ Microscope Components)

14Adjusting aid for HBO/XBO incident microscope illuminators

(Fig. 8)

 

 

 

 

 

 

 

 

 

The adjusting

aid

simplifies

optimum setting of

the

HBO

50/100

and

XBO

75 lamps

in

fluorescence

microscopy.

 

A mirror, directs the image

of the light arc to a

round

wind

visible

from

the

outside (matt

black filter).

Here,

the position

change of the focal point and its mirror image, performed by

adjusting

the

screws

on

the

microscope illuminator, can

be

viewed.

 

 

 

 

 

 

 

 

 

 

The

lamp is

set

optimally

if

the

image

and the

reflected

imag

of

the

light

arc

are

shown

in

the

window

centrally

and i

focus.

15

B 40 042 e 09/99

Stand

1

8

2

7

6

5

3

4

1

Connecting tube for microscope lamps incident light

2

Ventilation

slots

3

Socket

for

instrument plug

4

Connecting tube for microscope lamps transmitted light

5

Sockets for microscope lamps transmitted light

6

Toggle switch transmitted light/incident light

7

Sockets for microscope lamps incident light

8

Socket for Axiophot 2 Photo module

 

 

 

 

Fig. 9

Back

of

manual stand, also see Fig. 2

16

Back of manual stand

Key to Fig. 9 (back of manual stand)

1Connecting tube for microscope lamps incident light with

locking screw for dovetails to fix the lamps

2

Ventilation

slots (always

keep

clear;

minimum

distance:

 

15 cm)

 

 

 

 

 

3

Sockets for

instrument

plug

with

integrated

compart

 

ment

for instrument fuses (change

of → fuseschapter

 

Care,

Maintenance)

 

 

 

 

4Connecting tube for microscope lamps transmitted light,

with locking screw for dovetails to fix the lamps

5

Sockets

of

the integrated power unit for HAL 100 / trans

 

mitted

light microscope

lamps

 

6

Toggle

switch to

change between incident and transmit

 

ted

light.

 

 

 

 

 

The

short

delay after you push the switch helps prevent

 

you

from

hurting

your

eyes in

the unexpected bright light.

7

Sockets

of

the integrated power unit for HAL 100 incident

 

light

microscope

lamps

 

 

8

Socket

for

Axiophot 2

Photo

module

B 40 042 e 09/99

Stand

9

 

 

 

8

7

6

5

4 3

2

1

 

1

ON/OFF

switch

 

 

 

 

 

 

 

2

Shutter

switch incident light

 

 

 

 

 

3

Gear switchover to motor focusing

 

 

 

 

4

Turning to the right of the connected motorized components

5

Turning to the left of the connected motorized components

6

Coarse/fine drive

of

focusing

 

 

 

 

 

7

Rapid

stage

lowering

CHANGE (on both sides of the stand)

8

Rapid

stage

lowering

VIEW (on both sides of the stand)

9

Socket for connection of motorized condensers

 

 

 

 

 

 

 

 

 

 

 

Fig. 10

Motorized

stand

 

 

 

 

 

 

Stand

E/Stand mot

 

Specialities

 

 

o Integrated electronic

system

in the stand base

o Motor

focusing (only

MOT

stand)

Manual operation of these stands differs only slightly from the

operation

of the

manual

stand.

The

following

therefore

describes

only those stand functions which the instructions

for the

operation

of the

manual

stand

do not

cover.

The stand described here is equipped with all the available motorized and coded functions and may therefore differ from your stand configuration.

Note:;Microscope

Software

 

 

Some

of the motorized

functions

of the

stands can only (or

also)

be operated from the PC via the

software.

For a

description

of this

function,

please→

thsee chapter

Microscope Software.

The following numbers refer to Fig. 10 (motorized stand).

1ON/OFF switch

OInstrument switched off

IInstrument switched on

For checking the operating status,

the switch

lights

up green

in position I (for malfunctions→ seechapterCare, Mainte

nance).

 

 

 

When the Axioplan 2 imaging is

switched

on, not

only the

HAL 100 microscope lamp is supplied with power, but also the

Axiophot 2 Photo module, (if

it

is attached),

and the

motor

ized and coded

components.

 

 

 

 

Note:;Motorized

focusing and

all

the other

motorized

or

codable functions are only available when the instrument is switched on.

17

B 40 042 e 09/99

Stand

2

Shutter

switch

incident light

 

 

 

 

7, 8

Rapid

stage

lowering

CHANGE/VIEW

 

 

 

In

incident light

 

microscopy or when using an

externallyKnobs

on

both

sides

of

the

stand for rapid stage

lowering and

supplied

gas

discharge

lamp, it may be necessary

to blockmovingthe

 

it up again in the previous position.

 

 

 

light path without switching off

the

lamp. A

motorizedStage

lowering

 

 

 

 

 

 

 

 

 

shutter

is

used

 

for this, which interrupts the light

path

byPress

CHANGE knob7). (

 

 

 

 

 

 

swinging

 

in

a

diaphragm.

 

 

 

 

 

 

 

Stage will be lowered. The current focus setting is stored.

Normally,

the

shutter

is

opened and the lamp for

transmittedWhen

the

stage

is lowered, motorized

focusing

by

focus

light therefore switched

off.

 

 

 

 

 

 

ing

drive

is

switched

off.

 

 

 

 

 

A short press of switch2) blocks( the incident light and theMoving stage up

 

 

 

 

 

 

 

 

transmitted light

illuminator

is switched

on.

 

 

 

 

Press

VIEW knob8). (

 

 

 

 

 

 

 

If

you

want

to

combine

transmitted light

fluorescence

and

Stage

moves

up

and

the

stored focus

position

is

precisely

epi fluorescence

,

press

switch2 ( shutter)

for

more than

2

s.

reset.

 

 

 

 

 

 

 

 

 

 

 

 

The incident light or transmitted light beam path can then

be The

 

user friendly

design

of

this function enables

you

to

used. Pressing switch2) ( again returns to the change mode

operate

the

CHANGE/VIEW

keys with either your right

or

between

 

transmitted

and

reflected

light.

 

 

 

 

your

left

hand.

 

 

 

 

 

 

 

 

Note:;If you see no light in the transmitted or incident light technique, check by pressing switch2) whether( the shutter

is blocking the light path.

CAUTION!

3Gear switchover to motor focusing

Pressing the switch changes from coarse to fine drive, and vice versa (Fig. 10/3).

Risk of

injury and

instrument damage

Please do not insert your

hand or

any objects

between

the stage

and the

objective

when the

stage is moved upwards. The same applies to the downward motion: in that case, the space between condenser and stand base is reduced.

6

 

Motorized

focusing

drive

 

 

 

 

 

 

 

 

9

 

Socket

for

motorized

condensers

 

 

 

Focusing is manual by means

of a control knob (on

both Bothsides) the

voltage

supply

and

the

control

of motorized

con

that acts on an electronic encoder.

 

 

 

 

 

 

 

densers is made via this socket.

 

 

 

 

Difference:

To

change

between

coarse

and

fine

drive,

the

Switch

off

Axioplan 2

imaging

 

 

 

 

gear

switchover (Fig. 310/) must

be

operated.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Attach condenser to condenser carrier

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Connect plug of the condenser cable with 9socket)on (

 

4, 5

 

Turning

to

the

left

or

right

of

 

the

connectedstand

 

base.

 

 

 

 

 

 

 

 

motorized components(Fig. 10)

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Using the keys4) (and5)( attached to the right and

left of theNote:;When

the

Axioplan

2 imaging

is

switched

on,

stand you can operate two of the following

motorizedinstrument

initialization

is

performed

to allow

recognition

of

components:

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

the connected modules. This means that the system cannot

1.

Condenser

turret

 

 

 

 

 

 

 

 

 

 

 

 

recognize any modules which are connecafterd

the Axio

 

2.

Reflector

turret

 

 

 

 

 

 

 

 

 

 

 

 

 

 

plan 2

imaging has

been switched

on.

 

 

 

3.

Nosepiece

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

If

 

the condenser is to be

activated

via the

Microscope

control,

Key 4() turns the appropriate

control

element

of

the

compoit must

be activated

via

the

Setup

program

 

 

nent

to

the

right,

5key) to(

the

left.

 

 

 

 

 

 

 

(→ microscope control software).

 

 

 

 

Each time the microscope is switched on, the existence of

 

 

 

 

 

 

 

 

 

 

 

motorized components is "enquired" and recorded.

 

The

 

 

 

 

 

 

 

 

 

 

 

 

 

switching

function

 

for

the

first two

components

available

is

 

 

 

 

 

 

 

 

 

 

 

 

then assigned to keys4) and(5)(

in

the

above

sequence.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

The first component found is

always

assigned

to

the

 

keys

on

 

 

 

 

 

 

 

 

 

 

 

the right of the stand. For example, if your

instrument

 

is

 

 

 

 

 

 

 

 

 

 

 

equipped

with

the

 

motorized

components

1

to 44,) andkeys

(

 

 

 

 

 

 

 

 

 

 

 

 

 

 

(5)

on the

right

will

switch

the

condenser

turret

and

the

keys

 

 

 

 

 

 

 

 

 

 

 

on

 

the

left

switch

the

reflector

turret.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

18

B 40 042 e 09/99

Stand

7

6

 

1

2

3

4

5

1

CAN bus 1 connector

 

 

2

CAN bus 2 connector

 

 

3

TV

connector (input)

 

 

4

Impedance switching for TV port

 

 

5

Connector

for Optovar / zoom intermediate tube

6

RS

232

C

interface

 

 

7

SET

programming key

 

 

Fig. 11

Back

of

motorized stand

 

 

Back of stand E/MOT

1CAN bus61 connector

For connection of the Axiophot 2 photo module.

2CAN bus62 connector

These

interfaces

permit activation of the Axioplan 2 imaging

via

a

CAN

bus.

Normally,

the

signals

from a PC which m

be

equipped

with an appropriate driver board (CAN bus inter

face)

are

transferred to

the

Axioplan

2 imaging and read o

from

there.

The

second connector also

allows cascading. This

enables several series switched instruments to be activated by a PC.

The control commands for programming languages under

WINDOWSTM

are provided

by DLynamicLs (D

 

inkL ibraries)L

.

 

 

 

see→

 

 

 

 

 

 

For

details,

please

the

chapterInterface

 

Description or

get

in touch with

our service department.

 

5Connector for Optovar/LzoomL intermediate tube

The intermediate tube (coded or motorized) installed is con nected to the microscope via this connector using the cable supplied.

19

B 40 042 e 09/99

Stand

6

 

RS

232

C

interface

 

 

 

 

 

 

 

 

 

 

 

 

Coded

microscope

parts

 

 

 

 

 

 

 

 

 

 

 

 

 

 

The intermediate tube (coded or motorized) installed

is

con

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

nected to the microscope via this connector using

the

cableVarious

modules

 

of

the

Axioplan

2

imaging and

some

supplied.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

additional microscope components can be coded.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Codings is beneficial when the

microscope

software

is

 

used.

7

 

Programming

key

(SET)

 

 

 

 

 

 

 

 

 

 

The software can recognize coded parts of the microscope

This key only functions in conjunction with the light

manandger

display

their

position

or

status,

even

if

the

parts

involved

described

later.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

are not motorized. For example, filter wheels or intermediate

 

A

short

press

of

the

SET

programming

key

 

(<

3

s)

 

tubes can be coded without being motorized. In

this case,

the

 

 

stores

the

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

of the

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

software would record and display a manual change

 

current settings of the following microscope

parts,filter or optovar/zoom position. However, the microscope

 

 

provided

 

they

are

adjustable

 

by

motor

or

electronically

 

 

 

must

be appropriately

configured

if

it

is

to recognize

 

readable:

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

software

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

the coded

positions.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

o aperture

diaphragm

for

transmitted

light

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

The

configuration

is

performed

with

the

aid of

a

setup

pro

 

o luminous

 

field

 

diaphragm

for transmitted

light

 

 

 

 

 

 

 

gram

 

delivered

with

the

instrument.

Normally,

the

setup

 

o incident light

aperture

diaphragm

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

program

has

already

been implemented

in

the factory

 

prior

 

o filter

wheels

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

to

delivery

of

Axioplan

2

imaging.

 

 

 

 

 

 

 

 

 

 

 

 

o condenser

 

front

optics

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

(only

 

with

use of achromatic motorized dual

condenNote:;

Equipping the nosepiece

 

 

 

 

 

 

 

 

 

 

 

 

 

ser

0.5,

 

445341 0000 000, the so called

pathology The

microscope

software

 

must

be

started

via

the

PC

 

before

 

 

condenser

 

mot.)

 

 

 

 

 

 

 

 

 

 

 

 

 

 

the

nosepiece

is

fitted

with

objectives

(normally,

the

 

nose

One separate storage is possible for each position of

the

nosepiece

is

fitted

with

objectives

in the

factory

as

ordered). Fit the

piece.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

nosepiece mounts with the objectives displayed

for

these

The stored settings are automatically activated when

 

themounts by the program. If the objective to be

used

is

not

respective

positions

of

the

nosepiece

are

adopted.

 

 

displayed

by the

program,

the

setup

program

must

be

called

The stored values are retained after you switch

off

andthe

this

objective

input

for

a

mount

on

the

nosepiece.

 

instrument.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Always

 

check

whether

 

the

 

objective

position

and

 

the

If you have

 

changed

the

settings

of

the

current

position appropriateand

display

correspond

in

the

program.

The

same

now wish to call the

stored

settings,

all

you

have to

doappliesisturnfor the positions of the reflector

turret.

 

 

 

 

 

the nosepiece further and then return

it

to

the

previous

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

position.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Note:;Optovar

and

 

zoom

intermediate

tube

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

If you have inserted an Optovar intermediate tube

on

your

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Axioplan

2

 

imaging,

 

its

Light

Managers

settings

(field

 

stop

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

and

filter

wheels)

are

also

stored

depending

on

the position

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

of the motorized objective nosepiece.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

The

 

microscope

 

software

can

be

used

to

store

 

several

settings

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

for

each

mount

of the nosepiece. The settings are

stored

by

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

the

 

program

in

parameter

 

files

 

and

are

called

with

 

these

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

again.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

The

 

microscope

 

software

can

be

used

to

store

 

several

settings

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

for

each

mount

of the nosepiece. The settings are

stored

by

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

the

 

program

in

parameter

 

files

 

and

are

called

with

 

these

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

again.

20

B 40 042 e 09/99

Stand

Light manager

Press

and

hold

(> 3 s)

both upper switches

for fast stag

 

lowering (Fig. 12/1

left and right) at

the

same

time until

 

the

orange

LED

display

(Fig2. )12/lights

up

briefly.

 

Light manager means the microscope's ability to adjust the

This activates the so called "learning mode" with the dry

KÖHLER illumination setting automatically. At

the

same

time

objective

mentioned

above

as

reference

system.

 

 

 

 

the

image

illumination

is adjusted

at a

constant

color

tempe

If you want to adjust the

 

focusing speed in the fine driv

rature.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

press

 

both

left

switches

for

fast

lowering.

 

 

 

 

 

Configured settings are stored and are reproduced

automati

The upper switch causes a

 

coarse

focus

adjustment,

while

cally whenever you change the position of the

objective

the lower switch causes a

 

fine

focus

adjustment.

Holding

nosepiece.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

the

switch

slowly

changes

the

focusing

speed

in

the

fine

It is

not

necessary

to

connect the

microscope

to

a computerdrive.

You

can

now

choose

among approximately

40

dif

to

perform

this

function.

 

 

 

 

 

 

 

 

 

 

 

ferent

focusing

speed

parameters.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Focus again exactly on the specimen and press briefly

the

Essential

requirements

for

the light manager, however, are

upper right switch. This stores focusing speed and focus

the

 

motorized or

coded

nosepiece

and

the

possibility

of

position

for

the

reference

objective

currently

in

use.

 

 

motorized

adjustment

for

the

following

components:

 

 

Now switch to the objective with the next lower magnifi

o luminous

field

diaphragm for

transmitted

light

 

 

 

cation/aperture

and

adjust

focusing

speed

and focus

posi

 

 

 

tion

 

accordingly.

You

may

 

store

the

parameters

for

this

o aperture

diaphragm

for

transmitted

light

 

 

 

 

 

 

 

 

 

 

 

 

objective

by

pressing

briefly

 

the

 

upper

right

switch

for

fast

o filter

wheels

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

lowering.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

o condenser

front

optics

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Proceed with the other objectives accordingly. Oil

immer

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

(only

with

use

of

achromatic

motorized

dual condenser

sion

 

objectives

should

be

 

adjusted

last.

 

 

 

 

 

 

 

0.5, 445341 0000 000)

 

 

 

 

 

 

 

 

 

 

 

Finally press and hold (> 3 s) the upper right switch for f

o aperture

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

diaphragm

for

incident

light

(only for

incident

lowering

until the

orange

LED

display

lights

up

briefly.

This

 

light

techniques)

 

 

 

 

 

 

 

 

 

 

 

 

 

switches the "learn mode" off

and

you may

now

check

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

The

 

KÖHLER

illumination

setting

procedure

should

already

the

objectives'

parfocality

visually.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

have been performed prior to the inital use of your

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Axioplan 2 imaging→ (page 74). You should also make an

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

occasional check

to

ensure

that this setting has not changed.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

The factory programmed setting of the light manager

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

guarantees

a

practical

starting situation, from which you can

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

make

your

own

settings

and

then

store

them

with

the

SET

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

key.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Give

SET

key

(in

Fig7. )11/a

short

press

to

store

the

set

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

ting.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Automatic

 

balancing

of

focusing

speed

 

 

 

and

focus

 

position

(parfocality)

of

diffe6

 

 

 

rent

objectives

 

 

 

 

 

 

 

 

 

The lower part of the MOT

stand is equipped with a Harmonic

 

 

DriveTM

of high

precision which converts

the

hand

movement

 

 

 

in the focusing process into different sensitive vertical stage

 

 

 

movements (proportionally to

the objective's magnification).

 

1

2

 

 

 

 

 

 

 

 

 

 

 

Motor

focusing

also

compensates

minor

focus differences

1 Fast stage lowering

VIEW

 

 

 

 

 

 

 

 

 

 

which

always

occur between

the

objectives.

First,

however, 2 LED

 

 

the following

learning

process needs to

be

activated:

 

 

 

 

 

 

 

 

 

 

 

 

Fig. 12 Motorized stand

 

Focus dry objective exactly with the highest magnifica tion/aperture.

21

B 40 042 e 09/99

Microscope control software AxioVision Control

What does AxioVision Control offer?

AxioVision Control permits the remote control of the following Carl Zeiss microscopes from your PC:

o Axioplan

2 imaging / Axiophot 2

o Axiovert

100 M

o Axioskop

2 MOT

Remote control with AxioVision Control provides the following benefits:

o convenient remote control functionwith visual navigation aid

o flexible storage of microscope settingsand activation via

function

keys

 

 

o AxioMouse

permits microscope

operation via

PC mouse

o convenient

photomicrography with Axiophot

2 and

MC 200

 

 

 

 

o management of different user

profiles

 

System

requirements

 

 

 

 

 

Rcommended

hardware

and

software:

 

 

o Pentium

II

prozessor

300

MHz

 

 

 

o 128 MB

RAM

 

 

 

 

 

 

 

o 4 GB hard disk

 

 

 

 

 

 

o video adapter

1024

x 768

x 24

bits, 4

MB

for true color

o CD ROM drive

 

 

 

 

 

 

 

o 17" monitor

 

 

 

 

 

 

 

o Windows

 

98 /

Windows NT

4.0

Service

Pack

3

Handling

Minimum hardware and software requirements:

o Pentium

II

processor

233 MHz

 

 

 

o 32 MB

RAM

 

 

 

 

 

 

o 4 GB hard disk

 

 

 

 

 

 

o video

adapter 1024

x

768

x 24

bits,

4 MB

for true color

o CD ROM drive

 

 

 

 

 

 

o 17"

monitor

 

 

 

 

 

 

o Windows

95

 

 

 

 

 

 

Note:;Detailed

information

on

the

microscope

control

software

is

contained

in

the

AxioVision

Control manual

B 40 623

(Cat. No. 000000 1068 563)

 

 

 

22

B 40 042 e 09/99

Microscope control software AxioVision Control

3

1

 

2

 

 

1

RS 232

C

connection

2

CAN bus

connection

3

Socket for

Axiophot

Fig. 13

Motorized

stand (back)

1

1

Serial

interface RS

232

 

2

Power

socket

 

 

3

2 CAN bus sockets

4 3

2

4

Spare

socket

 

 

Electrical

 

connections

 

 

 

 

 

 

 

 

 

 

 

Microscope

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

The coupling

of

the

Axioplan

2

with

the

PC or

the

noteboo

is

performed via

the

RS

232

C serial

interface1) (Figusing.13/

 

 

 

a

connecting

cable.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Axiophot

2

photo

module

 

 

 

 

 

 

 

 

 

 

 

The electrical

connections

are

on

the

back

of the

Axiophot

2

photo

module:

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

o CAN bus

socket

for

cable

connection to the stand and

 

 

other

microscope

components

(Fig. 314/)

 

 

 

 

 

 

 

o RS

232

serial interface

for

the connection of

a

PC

or

note

 

book

(Fig. 14/1)

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

o Spare

socket

(Fig. 14/4)

 

 

 

 

 

 

 

 

 

 

 

 

 

o Power

socket

(Fig. 14/2)

 

 

 

 

 

 

 

 

 

 

 

 

 

Connection

to

the

manual

stand

 

 

 

 

 

 

 

 

 

 

Connect

the

supplied

RS

232

C

connection

cable

to

the

 

serial

interface of

the photo module (Fig1) . and14/ to the

 

 

 

serial

interface

of

your

notebook/PC.

 

 

 

 

 

 

 

 

Connect

the internal voltage supply cable supplied to the

 

 

POWER

socket

(Fig. 14/2)

of

the photo module and to

 

 

 

socket

(Fig. 13/3)

at the

back

of

the

stand.

 

 

 

 

 

 

Tighten

all

the

safety

screws

on

the

plugs.

 

 

 

 

 

Connection

to

the

stand E/MOT

 

 

 

 

 

 

 

 

 

 

Use

the

RS

232

C cable

supplied

to

connect

the

serial

in

 

terface

 

of

the

photo

module

(Fig1). 14/or the

 

stand

 

 

 

(Fig. 13/1)

to

the

serial

interface

of your notebook/PC.

 

 

 

Connect

the internal voltage supply cable supplied to the

 

 

POWER

socket

(Fig. 14/2)

of

the photo module and to

 

 

 

socket

(Fig. 13/3)

at the

back

of

the

stand.

 

 

 

 

 

 

Connect

photo module and stand by connecting the CAN

 

 

bus

cable

to

the

appropriate

CAN

bus

sockets 3 (Fig. 14/

 

 

and

Fig. 13/2).

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Tighten

all

the

safety

screws

on

the

plugs.

 

 

 

 

 

Fig. 14 Electrical connections of the Axiophot 2

23

B 40 042 e 09/99

System Overview

Condensers

DIC prism II / 1.4

(445375 0000 000)

DIC prism III / 1.4

(445376 0000 000)

Achromatic aplanatic universal condenser 0.9 H

(000000 1035 632)

Achromatic aplanatic universal condenser 0.9 H D Ph

(445436 0000 000)

Achromatic aplanatic universal condenser 0.9 H D Ph DIC

(445439 0000 000)

Achromatic switching condenser 0.9 H

(000000 1017 688)

Achromatic switching condenser 0.9 H D Ph

(000000 1017 690)

DIC prism I / 0.9 (with polarizer)

(000000 1005 002)

DIC prism II / 0.9 (with polarizer)

(000000 1005 003)

DIC prism III / 0.9 (with polarizer)

(000000 1005 004)

DIC prism I / 0.9

(000000 1004 901)

DIC prism II / 0.9

(000000 1004 902)

DIC prism III / 0.9

(000000 1004 903)

Front lens 0.9 / d=2.9 Pol

(445331 0000 000)

Front lens 0.6 / d=4.2 LD, a=6.8 mm

(445330 0000 000)

 

Achromatic aplanatic swing in

 

Achromatic aplanatic condenser 1.4 H Ph DIC,

condenser system 0.24/d=10.7

 

(445325 9901 000)

Brightfield insert

(445453 0000 000)

 

 

(445467 0000 000)

 

 

 

UV condenser 0.6 Pol for Axioplan 2 imaging Pol

 

 

(445460 0000 000))

 

Achromatic condenser 0.8 H

Achromatic condenser 0.8 H mot.

(445443 0000 000)

(445444 0000 000)

 

Achromatic condenser 0.8 H D Ph mot. Achromatic condenser 0.8 H D Ph DIC (445446 0000 000)

(445445 0000 000)

Achromatic dual

condenser 0.5

Achromatic dual

condenser 0.5 mot.

(445340 0000 000)

 

(445341 0000 000)

 

Darkfield illuminator

(445314 9901 0000 000)

Ultra condenser 1.2/1.4 (0.75 1.0)

Dry darkfield condenser

(465500 0000 000)

0.8/0.95 (0.6 0.75)

 

(465505 0000 000)

Condenser holder Z

(445323 0000 000)

24

B 40 042 e 09/99

Zeiss Axiophot 2 User Manual

System Overview

Stage carriers, Stages, Condenser carriers

Specimen holder with spring clip R

(453533 0000 000)

Rotary, centerable mechanical stage 75x50 _/ R240

(453502 9904 000)

Mechanical stage 75x50 R with

electrical vernier scale

(453507 0000 000)

Adapter for mineralogy specimen mount

(453541 0000 000)

Specimen holder A

(000000 1046 521)

Specimen holder A

(453539 0000 000)

Specimen holder for mechanical stage 000000 1063 835/836

(000000 1046 521)

Mechanical stage 75x50 L

Mechanical stage 75x50 R

with ergo drive

with ergo drive

(000000 1063 836)

(000000 1063 835)

Rotary stage Pol

(453550 0000 000)

with object guide Pol

(453560 0000 000)

Mechanical stage 75x50 mot with control unit

(000000 1025 145)

Specimen holder with special fixation for the mounting of two specimens

(453538 0000 000)

Specimen holder D Pol

(453563 0000 000)

Specimen holder A Pol

(453564 0000 000)

optional

 

 

Joystick

for 2 axes

Fixed stage carrier

(000000 1033 996)

(452121 0000 000)

or

 

 

 

Coaxial

electronic drive

 

(000000 1034 960)

 

Lowerable and removable stage carrier

(452122 0000 000)

Centerable condenser carrier with height adjustment on both sides

(452125 0000 000)

Attachable stage carrier ZA

(451826 0000 000)

Centerable condenser carrier with height adjustment on both sides for removable stage carrier

(452127 0000 000)

25

B 40 042 e 09/99

System Overview

Stand, Tubes, Eyepieces, Filters, Reflectors

Double video adapter

(000000 1058 640)

Binocular phototube with height adjustment

(452146 0000 000)

 

 

 

 

Binocular

phototube

with

 

 

 

 

sliding prism 30/25 (100/100/50:50)

 

 

 

 

(452143 0000 000)

 

Eyepiece

Pl

10x/25

Br.

 

 

 

(444033 0000 000)

 

 

 

 

Eyepiece Pl 10x/25 Br. foc.

 

 

 

(444034 0000 000)

 

 

 

 

Eyepiece

Pl

16x/16

Br.

 

 

 

(444053 0000 000)

 

 

 

 

Eyepiece Pl 16x/16 Br. foc.

 

 

 

(444054 0000 000)

 

 

 

 

Eyepiece E Pl 10x/25 Br. foc.

 

 

 

(444234 0000 000)

 

Binocular

tube with

 

Eyepiece E Pl 10x/23 Br. foc.

two TV

ports _30/25

(100/50:50/30:70)

(444235 0000 000)

 

(452145 0000 000)

 

Reflector module H

(000000 1046 274)

Reflector module D

(000000 1046 276)

Reflector module DIC

(000000 1046 277)

Reflector module DIC red I

(000000 1046 278)

Reflector module Pol

(000000 1046 279)

Reflector module Fl

(000000 1046 281)

Optovar module 2.5x

(000000 1046 282)

Optovar module 1.6x

(000000 1046 283)

Optovar module 1.25x

(000000 1046 284)

Analyzer module D

(000000 1046 958)

Luminous field diaphragm insert D

(452160 0000 000)

Luminous field diaphragm insert D mot.

(452161 0000 000)

Double TV tubus mot.

(000000 1054 146)

Reflector module H

(000000 1046 274)

Reflector module Fl

(000000 1046 281)

Filter dia. 25 mm and beam splitter

(according to price list)

Upper stand part of Axioplan 2 imaging

(000000 1050 687)

Upper stand part of Axioplan 2 imaging mot.

(000000 1050 688)

Incident light illuminator

(452171 0000 000)

Incident light illuminator Fl

(452170 0000 000)

Filter slider A

(452174 0000 000)

Integrated 12 V/100 W power unit

(452185 0000 000)

Transmitted light illumination

(452150 0000 000)

Axioplan 2 imaging stand base

(000000 1060 417)

Axioplan 2 imaging mot. stand base

(000000 1052 299)

Axioplan 2 imaging El. stand base

(000000 1052 300)

Filter magazine D

(452155 0000 000)

Filter magazine D photo

(452157 0000 000)

Filter magazine D mot.

(452156 0000 000)

26

B 40 042 e 09/99

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