Zeiss Axiolab A User Manual

5 (2)

Axiolab A

Reflected-Light

Microscope

Operating Manual

Carl Zeiss

Axiolab A

 

 

Knowledge of this instruction manual is necessary for device operation. Please get familiar with its contents and especially the precautions for safe device operation.

Changes due to further technical development are reserved; this manual is not covered by an update service.

©Unless expressly authorized, forwarding and duplication of this document, utilization and communication of its contents are not permitted. Violations will entail an obligation to pay compensation.

All rights reserved in the event of granting of patents or registration of an utility model.

Issued by:

Carl Zeiss

 

 

Microscopy

 

D-07740 Jena

 

Phone:

++49-36 41 64-16 16

 

Telefax:

++49-36 41 64-31 44

 

E-mail:

micro@zeiss.de

 

Internet:

www.zeiss.de/micro

Number of this operating manual: B 40-015 e

Date of issue: 06/99

II

B 40-015 e 06/99

Axiolab A

 

Carl Zeiss

 

 

 

 

CONTENTS

 

 

 

Page

 

INTRODUCTION .................................................................................................................

I

 

Title Page ............................................................................................................................

I

 

Copyright ...........................................................................................................................

II

 

Contents ............................................................................................................................

III

 

List of Illustrations...............................................................................................................

V

 

Notes ................................................................................................................................

VI

 

Hints on Instrument Safety................................................................................................

VII

 

Overall View of the Axiolab A Reflected-Light Microscope....................................................

X

1

DESCRIPTION.................................................................................................................

1-1

1.1Name; Intended Application,............................................................................................1-2

1.2

Instrument Description.....................................................................................................

1-2

1.3

Microscope Configurations and Modules..........................................................................

1-5

1.4

Function Elements (see Fig. 1-5, after this table) .............................................................

1-12

1.5

Objectives......................................................................................................................

1-18

1.6

Eyepieces.......................................................................................................................

1-19

1.7

Stage Micrometers and Eyepiece Reticles........................................................................

1-20

1.8

Technical Data...............................................................................................................

1-22

2

START-UP.......................................................................................................................

2-1

2.1

Unpacking the Instrument................................................................................................

2-3

2.2

Screw in Objectives..........................................................................................................

2-3

2.3

Insertion of Eyepieces ......................................................................................................

2-4

2.3.1

Insertion of Eyepiece Reticle .............................................................................................

2-4

2.3.2

Compensation of Ametropia when Eyepiece Reticles are used ..........................................

2-5

2.4

Setting of Interpupillary Distance......................................................................................

2-5

2.5

Attachment of Reflected-Light Halogen Illuminator...........................................................

2-6

2.6

Retrofit the Transmitted-Light Halogen Illumination ..........................................................

2-6

2.6.1

Switch on the Transmitted-Light Halogen Illuminator........................................................

2-7

2.7

Equipment of Filter Slider .................................................................................................

2-7

2.8

Set the Luminous-Field Diaphragm ...................................................................................

2-8

2.9

Connecting the Instrument to the Line .............................................................................

2-8

B 40-015 e 06/99

III

Carl Zeiss

Axiolab A

 

 

 

 

Page

3

OPERATION...................................................................................................................

3-1

3.1

Switch on the Instrument ................................................................................................

3-3

3.2

Illumination and Contrasting Techniques..........................................................................

3-4

3.2.1

Setting of Reflected-Light Brightfield................................................................................

3-4

3.2.2

Setting of Reflected-Light Polarization..............................................................................

3-5

3.2.3

Setting of Transmitted-Light Polarization with extended Polarization Equipment ...............

3-5

3.2.4

Setting of Epi-Fluorescence............................................................................................

3-13

3.2.5

Setting of Transmitted-Light Brightfield (KÖHLER Illumination)........................................

3-14

3.3

Attachment of Microscope Stages and Specimen Holders...............................................

3-15

3.3.1

Attachment of Pol Rotary Stage.....................................................................................

3-16

3.4

Use of Polished Section Attachment...............................................................................

3-17

3.5

Photomicrography and Videomicroscopy........................................................................

3-18

3.5.1

Attachment of Photomicrography Equipment ................................................................

3-19

3.5.2

Attachment of Adapters for Video Cameras...................................................................

3-22

3.6

Insertion of 8× Drawing Eyepiece...................................................................................

3-24

4

CARE, TROUBLESHOOTING AND SERVICE ...................................................................

4-1

4.1

Maintenance of the Instrument .......................................................................................

4-3

4.2

Troubleshooting ..............................................................................................................

4-4

4.3

Requesting Service ..........................................................................................................

4-6

 

ANNEX...........................................................................................................................

A-1

 

List of Abbreviations........................................................................................................

A-3

 

Certification in Accordance with DIN EN ISO 9001 / DIN EN 46001 ...................................

A-5

 

EC Conformity Declaration ..............................................................................................

A-7

IV

B 40-015 e 06/99

Axiolab A

Carl Zeiss

 

 

LIST OF ILLUSTRATIONS

 

 

Page

Fig. 1-1

Axiolab A main modules ..................................................................................................

1-3

Fig. 1-2

Optical design of the Axiolab A with transmitted-light equipment.....................................

1-4

Fig. 1-3

Axiolab A microscope configurations................................................................................

1-6

Fig. 1-4

Axiolab A accessories..............................................................................................

1-8, 1-10

Fig. 1-5

Axiolab A function elements .................................................................................

1-16, 1-17

Fig. 2-1

Unpacking the instrument................................................................................................

2-3

Fig. 2-2

Screw in objectives ..........................................................................................................

2-3

Fig. 2-3

Insertion of eyepiece reticle..............................................................................................

2-4

Fig. 2-4

Attachment of reflected-light halogen illuminator.............................................................

2-6

Fig. 2-5

Equipment of filter slider..................................................................................................

2-7

Fig. 2-6

Setting the luminous-field diaphragm...............................................................................

2-8

Fig. 2-7

Connecting the instrument to the line..............................................................................

2-9

Fig. 3-1

Switch on the instrument.................................................................................................

3-3

Fig. 3-2

Setting of reflected-light brightfield..................................................................................

3-4

Fig. 3-3

Setting of reflected-light polarization ...............................................................................

3-5

Fig. 3-4

Centering of objectives ....................................................................................................

3-6

Fig. 3-5

Setting of transmitted-light polarization ...........................................................................

3-7

Fig. 3-6

Determine the nγ ' vibration direction using the example of an artificial fiber......................

3-8

Fig. 3-7

Schematic diagram of the color chart in addition and subtraction position ........................

3-8

Fig. 3-8

Determine the optical character of crystals .....................................................................

3-11

Fig. 3-9

Setting of epi-fluorescence.............................................................................................

3-13

Fig. 3-10

Setting of transmitted-light brightfield ...........................................................................

3-14

Fig. 3-11

Stage selection ..............................................................................................................

3-15

Fig. 3-12

Changing the microscope stage .....................................................................................

3-15

Fig. 3-13 Pol rotary stagesetting of specimen mount and stop ....................................................

3-16

Fig. 3-14

Use of polished section attachment................................................................................

3-17

Fig. 3-15 Attach various camera systems to the Axiolab A phototube ............................................

3-18

Fig. 3-16

Attachment of SLR camera, e.g. CONTAX 167 MT .........................................................

3-19

Fig. 3-17

Attachment of MC 80 DX...............................................................................................

3-21

Fig. 3-18

Insertion of 8× drawing eyepiece....................................................................................

3-24

B 40-015 e 06/99

V

Carl Zeiss

Axiolab A

 

 

Fig. 4-1

Changing the fuses .........................................................................................................

4-4

Fig. 4-2

Changing the 6 V, 30 W reflected-light halogen illuminator .............................................

4-5

Fig. 4-3

Changing the 6 V, 30 W transmitted-light halogen lamp..................................................

4-6

NOTES

The figures integrated in the text each have a figure number and a caption, e.g. ”Figure 2-7” signifies: the figure in Section 2 with the serial number 7. In each figure, details discussed in the text have been assigned with a reference line marking and an item number. In the running text, ”Mains cable (2-7/1)” signifies: in Figure 7 of Section 2, the mains cable is marked with the item number 1.

Refer to the annex for explanations of the abbreviations.

This instruction manual refers to the Axiolab A microscope equipment including accessories (see page 1-5 following).

This manual can also be used for other instrument versions.

VI

B 40-015 e 06/99

Axiolab A

Carl Zeiss

 

 

Notes on Instrument Safety

The Axiolab A reflected-light microscope was designed, produced and tested in compliance with DIN 61010-1 (IEC 1010-1), Safety requirements for electrical measuring, control and laboratory instruments, and meets the requirements of appendix I of directive 73/23/EC and the relevant CSA and UL directives. The instrument meets the requirements of the EC directive 89/336/EC and the EMC legislation of November 9th 1992. This operation manual includes information and warnings which must be observed by the user.

The following warning and information symbols are used in this manual:

NOTE

This symbol is a warning which you must observe under all circumstances.

CAUTION

This symbol is a warning which indicates a hazard to the instrument or instrument system.

CAUTION

This symbol is a warning which indicates a hazard to the user of the instrument.

CAUTION

Hot surface!

CAUTION

Disconnect the instrument from the line before opening it!

B 40-015 e 06/99

VII

Carl Zeiss

Axiolab A

 

 

The Axiolab A microscope, including original accessories, may only be used for the microscope techniques described in this manual.

Particular attention must be paid to the following warning notes:

The manufacturer cannot assume any liability for any other applications, possibly also involving individual modules or single parts. This also applies to all service or repair work which is not carried out by authorized service personnel. Furthermore, this forfeits all the claims against warranty.

The power plug must be inserted in a socket featuring a grounding (earth) contact. The grounding effect must not be made ineffective by an extension cable which does not have a protective ground wire.

If it is established that the protection measures are no longer effective, the instrument must be switched off and safeguarded against inadvertent operation. Please contact your local Carl Zeiss service agency or the Carl Zeiss microscopy service for the repair of the instrument.

The wide range power unit which is integrated in the stand of the microscope permits the use of line voltages in the range between 100 and 240 V AC ± 10%, 50 - 60 Hz, without the need for the voltage to be changed at the instrument.

Always disconnect the instrument from the line before opening the instrument and before changing the fuses.

Make sure to use only fuses of the rated power required. The use of makeshift fuses and the short-circuiting of the fuse holders are not permitted.

The Axiolab A microscope is not equipped with any special devices for protection from substances which are corrosive, toxic, radioactive or otherwise hazardous to health. All the legal regulations for accident prevention, particularly those in the respective countries, must be observed when handling such substances.

Avoid touching the hot lamp housing. Always pull the power plug before changing the lamps and allow the instrument to cool down for approx. 15 mins.

VIII

B 40-015 e 06/99

Axiolab A

Carl Zeiss

 

 

Dust and dirt can impair the performance of the instrument. Therefore, the instrument must be protected against these influences as far as possible, and covered with the dust cover if it is not used for longer periods of time. Always check whether the instrument is switched off before you cover it.

Placing objects against or covering ventilation slats can lead to a build-up of heat which will damage the instrument and, in extreme cases, cause a fire. Always keep the ventilation slats clear and make sure that no objects enter the instrument through the ventilation slats.

The instruments must be operated by trained personnel only who must be aware of the possible danger involved with microscopy and the relevant application. The Axiolab A is an optical precision instrument which can be impaired in its performance or damaged when handled improperly.

Notes on warranty

The manufacturer guarantees that the instrument has no material and production defects when delivered. You must inform us of any defects immediately and we must do anything to minimize the damage. If the manufacturer is informed of such a defect, he is obliged to remove it; it is his decision whether he does this by repairing the instrument or by delivering an instrument free of any defect. No guarantee is provided for defects caused by natural wear (wearing parts in particular) and improper use.

The instrument manufacturer is not liable for damage caused by faulty operation, negligence or any other meddling with the instrument, particularly the removal or replacement of instrument components, or the use of accessories from other manufacturers. This forfeits all the claims against warranty.

With the exception of the work specified in this manual, no maintenance or repair of the Axiolab A may be performed. Repairs may only be performed by Carl Zeiss service staff or specially authorized personnel. Should any defect occur with the instrument, please get in touch with your local Carl Zeiss agency.

B 40-015 e 06/99

IX

Carl Zeiss

Axiolab A

 

 

Overall View of the Axiolab A Reflected-Light Microscope

X

B 40-015 e 06/99

Axiolab A

Carl Zeiss

 

 

DESCRIPTION

Contents

1

DESCRIPTION.................................................................................................................

1-2

1.1Name; Intended Application,............................................................................................1-2

1.2

Instrument Description.....................................................................................................

1-2

1.3

Microscope Configurations and Modules..........................................................................

1-5

1.4

Function Elements (see Fig. 1-5, after this table) .............................................................

1-12

1.5

Objectives......................................................................................................................

1-18

1.6

Eyepieces.......................................................................................................................

1-19

1.7

Stage Micrometers and Eyepiece Reticles........................................................................

1-20

1.8

Technical Data...............................................................................................................

1-22

B 40-015 e 06/99

1-1

Carl Zeiss

Axiolab A

 

 

1 DESCRIPTION

1.1Name; Intended Application,

Manufacturer's name:

Upright reflected-light microscope for routine examinations

Within the product family of upright reflected-light microscopes, the Axiolab A fits in as follows:

Routine microscopes

Research microscopes

Axiolab A

Axioplan 2 imaging

− Axiotech / Axiotech vario

Axiophot 2

− Axioskop 2 / Axioskop 2 MOT

− Axiotron 2 / Axiotron DUV

 

 

− Axioskop 2 FS / Axioskop 2 FS MOT

The Axiolab A reflected-light microscope is a light microscope suitable for use in all areas of research and industry involving opaque material, e.g. in

-

-

-

metallography (metallic and compound materials)

engineering mineralogy (ceramics, building materials, slags and ashes)

production inspection/receiving inspection (plastics, papers and textiles)

The Axiolab can also be equipped with a transmitted-light facility for the examination of transparent material samples.

1.2Instrument Description

The Axiolab A microscope is a high-performance routine microscope. Due to its compact design and clearly arranged controls, the instrument also meets high demands on user-friendliness.

-Microscope stand (1-1/1): diecast component with low-lying center of gravity and, therefore, high stability.

-Coaxial coarse/fine drive (1-1/2): focusing function acting on the specimen stage

-Stage: optionally as fixed version (1-1/3), as glide stage (1-1/5) or as mechanical stage (1-1/4)

-5-position objective nosepiece (1-1/6) with W 0.8 × 1/36" thread

-Reflected-light illuminator H (1-1/7) with permanently attached binocular phototube A H (1-1/8), two E-PL 10× /20 Br. and E-PL 10× /20 Br. foc. eyepieces , and camera port (1-1/9)

-Wide-range power supply in the stand can be set to line voltages between 100 and 240 V AC

-Reflected-light halogen illuminator (1-1/10) with 6 V, 30 W halogen lamp

The Axiolab A microscope features a modular design, i.e. standard modules can be easily replaced with alternative modules, or further accessories can be added. The carrier element for this purpose is the microscope stand (1-1/1), in which the power supply unit is also integrated. The new wide-range power supply in the stand can be set to line voltages between 100 and 240 V AC by the user himself.

1-2

06/99 B 40-015 e

Axiolab A

Carl Zeiss

 

 

Fig. 1-1 Axiolab A main modules

The specimen stage can be designed

-as fixed stage (1-1/3) with stage clamps,

-as glide stage (1-1/5) with stage clamps, 25 × 25 mm travel range, or

-as mechanical stage (1-1/4) 75 × 35 R/A with ceramic surface and short drive.

Both the fixed stage and the glide stage can be locked in a top or a bottom stop. The maximum specimen height is 45 mm.

The mechanical stage is a component of the transmitted-light version of the Axiolab A and permits the particularly sensitive moving of transparent objects. For opaque specimens, this mechanical stage can be equipped with a special specimen holder, permitting the maximum specimen height of 18 mm. Controls within easy reach and the user-friendly viewing height of 440 mm with a 30° viewing angle ensure fatigue-free work.

B 40-015 e 06/99

1-3

Carl Zeiss

Axiolab A

 

 

The reflected-light halogen illuminator (1-1/10) is equipped with a 6V 30 W halogen lamp as a standard. Its luminous intensity (lamp voltage) is continuously variable via the relevant control. The correct color temperature for color photography (3200 K) is automatically reached in the end stop. The XBO 75 or HBO 50 illuminators can also be attached to the Axiolab A for examinations requiring a high illuminance. Other features: adjustable aperture diaphragm and homogeneous illumination of object fields of up to dia. 8 mm.

Fig. 1-2 Optical design of the Axiolab A with transmitted-light equipment

1-4

06/99 B 40-015 e

Axiolab A

Carl Zeiss

 

 

The optical performance of the Axiolab A can be ideally matched to the relevant requirements by using various objectives which are inserted into a 5-position nosepiece (1-1/6).

The camera port (1-1/9) attached to the binocular reflected-light tube (1-1/8) is available for the documentation of microscope images. Microscope and video accessories are attached via a wide variety of specific adapter components and modules.

Pulling out or pushing in the pushrod on the binocular tube allows you to switch between observation and photomicrography/videomicroscopy. Switchover is 100% in each case, i.e. simultaneous observation and documentation is not possible.

The use of specific sliders in the reflected-light illuminator H allows observation in polarization contrast. Epi-fluorescence observation is possible with a special fluorescence configuration.

1.3Microscope Configurations and Modules

Depending on the application, the following configurations of the Axiolab A microscope are recommended:

- Axiolab A for reflected-light brightfield with fixed stage and Epi-objectives

Cat. No. 490960-9804-000

- Axiolab A for reflected-light brightfield with glide stage and Epi-objectives

Cat. No. 490962-9804-000

-Axiolab A for reflected-light brightfield and transmitted-light examinations with mechanical stage

Cat. No. 490961-9804-000

B 40-015 e 06/99

1-5

Carl Zeiss

 

 

Axiolab A

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Fig. 1-3 Axiolab A microscope configurations

1-6

06/99 B 40-015 e

Axiolab A

Carl Zeiss

 

 

 

Axiolab A microscope configurations

Cat. No.

Fixed

Glide

Mechani-

 

 

 

stage

stage

cal stage

 

 

 

 

 

 

1

Configurations

 

 

 

 

1

Microscope Axiolab A with fixed mechanical stage

490960-9804-000

 

 

2

Microscope Axiolab A with glide stage

490962-9804-000

 

 

3

Microscope Axiolab A with mechanical stage and

490961-9804-000

 

 

 

additional transmitted-light equipment

 

 

 

 

 

Selected modules for configuration 1

 

 

 

 

1.1

Microscope stand Axiolab A

 

450909-0000-000

 

 

1.2

Binocular phototube 30°/20 A H (100 vis/

450962-0000-000

 

 

 

100 doc) with reflected-light illuminator H

 

 

 

 

2.1

Eyepiece E-PL 10× /20 Br.

 

444231-9901-000

2.2

Eyepiece E-PL 10× /20 Br. foc.

444232-9902-000

3.1

Epiplan objective 5× /0.13 ∞ /-

442920-0000-000

3.2

Epiplan objective 10× /0.20 ∞

/-

442930-0000-000

3.3

Epi objective 20× /0.40 ∞ /0

 

442941-0000-000

3.4

Epiplan objective 50× /0.70 ∞

/0

442950-0000-000

 

3.5

Achroplan objective 40× /0.65 ∞ /017

440050-0000-000

 

 

4.1

Filter slider,

 

 

 

is a component of binocular phototube 30°/20 A H

450962-0000-000

 

 

 

 

(100 vis/100 doc) with reflected-light illuminator H

 

 

 

 

4.2

Attenuation filter 0.06, dia. 32

467848-9001-000

5

Dust cover slider (5 pcs.)

 

 

6.1

6 V, 30 W halogen illuminator

447206-9901-000

6.2

6 V, 30 W halogen lamp

 

000000-0402-943

7

Dust cover G

 

459306-0000-000

8.1

Mains cable with European plug

 

8.2

Mains cable with American flat plug

 

on request

on request

on request

8.3

Fuse insert T 0.8 A; 250 V; 5 × 20 mm

000000-0127-019

 

(not shown)

 

 

 

 

 

9

Accessory case

 

 

Part of the microscope configuration

B 40-015 e 06/99

1-7

Carl Zeiss

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Axiolab A

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Fig. 1-4 Axiolab A accessories (1 of 2)

1-8

06/99 B 40-015 e

Axiolab A

Carl Zeiss

 

 

 

Axiolab A accessories

 

Cat. No.

Fixed

Glide

Mechani-

 

 

 

 

 

 

stage

stage

cal stage

 

 

 

 

 

 

 

 

 

 

STAGES

 

 

 

 

 

 

 

1.1

Glide stage with stage carrier

 

 

453509-0000-000

!

 

 

1.2

Mechanical stage 75 ×

35 R/A with ceramic

453517-9901-000

+

+

 

 

surface and short drive, plus:

 

 

 

 

 

 

1.2.1

Specimen holder with spring clip R

 

473448-0000-000

+

+

 

1.2.2

Specimen holder A

 

 

 

433411-0000-000

+

+

 

1.3

Polished specimen attachment

 

 

453575-0000-000

!

!

 

 

OBJECTIVES

 

 

 

 

 

 

 

2.1

Epiplan objective 100×

/0.75 ∞ /0

 

442980-0000-000

 

 

!

2.2

Epiplan-Neofluar objective 2.5×

/0.075 ∞ /-, plus:

442310-0000-000

!

!

!

2.2.1

2,5× Antiflex cap

 

 

 

444922-0000-000

+

+

+

2.3

Epiplan-Neofluar objective 5× /0.15 ∞ /-

442320-0000-000

!

!

!

2.4

Epiplan-Neofluar objective 10×

/0.30 ∞

/0

442330-0000-000

!

!

!

2.5

Epiplan-Neofluar objective 20×

/0.50 ∞

/0

442340-0000-000

!

!

!

2.6

Epiplan-Neofluar objective 50×

/0.85 ∞

/0

442350-0000-000

 

!

!

 

for covered specimens:

 

 

 

 

 

 

2.7

Achroplan objective 20× /0.45 ∞

/0.17

 

440040-0000-000

 

 

!

2.8

Achroplan objective 40× /0.65 ∞

/0.17

 

440050-0000-000

 

 

!

2.9

Achroplan objective 100× /1.25 ∞ /0.17 oil

440080-0000-000

 

 

!

 

plus:

 

 

 

 

 

 

 

2.9.1

518 N immersion oil, 20 ml oiler

 

444950-0000-000

 

 

+

3.1

Eyecup, folding

 

 

 

444801-0000-000

!

!

!

3.2

8× drawing eyepiece

 

 

 

444128-0000-000

!

!

!

4

Stage micrometer for incident light 5+100/100y

474027-0000-000

!

!

!

 

D = 0

 

 

 

 

 

 

 

5

Equipment for qualitative polarization in incident

 

!

!

!

 

light

 

 

 

 

 

 

 

5.1

Polarizer slider P

 

 

 

453608-0000-000

+

+

+

5.2

Analyzer slider A, rotary

 

 

453686-0000-000

+

+

+

5.3

Compensator slider λ -sub

 

 

453706-0000-000

+

+

+

6

Equipment for Epodye fluorescence

 

 

!

!

!

6.1

Filter slider FL (for dia. 25 filters)

 

446478-0000-000

+

+

+

6.2

Excitation filter BP 450 ... 490, dia. 25

 

447722-0000-000

+

+

+

6.3

Barrier filter LP 520,

25

 

 

447737-0000-000

+

+

+

B 40-015 e 06/99

1-9

Zeiss Axiolab A User Manual

Carl Zeiss

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Axiolab A

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Fig. 1-4 Axiolab A accessories (2 of 2)

1-10

06/99 B 40-015 e

Axiolab A

Carl Zeiss

 

 

 

Axiolab A accessories

Cat. No.

Fixed

Glide

Mechani-

 

 

 

stage

stage

cal stage

 

 

 

 

 

 

7

Retrofit package for transmitted-light

 

!

!

 

 

illumination (can only be fitted by service

 

 

 

 

 

personnel), consisting of:

 

 

 

 

7.1

- Mechanical stage 75 × 35 R/A with ceramic

453517-9901-000

+

+

 

 

surface

 

 

 

 

7.2

- Specimen holder (1) with spring clip R

473448-0000-000

+

+

 

7.3

- Transmitted-light illumination, consisting of:

450951-0000-000

+

+

 

7.3.1

- 6 V, 30 W halogen lamp

000000-0402-943

+

+

!

7.3.2

- Luminous-field diaphragm with iris

 

+

+

+

7.4

- ABBE condenser 0.9/1.25

445302-0000-000

+

+

+

 

For enhanced incident light intensity

 

 

 

 

 

requirements

 

 

 

 

8.1

XBO 75 (Xenon) illuminator

487202-9804-000

!

!

!

8.2

HBO 50 (Hg, 230 V) illuminator

487201-9804-000

!

!

!

 

HBO 50 (Hg, 115 V) illuminator

487201-9904-000

 

 

 

9

MC 80 DX microscope camera for

496065-9804-000

!

!

!

 

24 × 36 mm, consisting of:

 

 

 

 

9.1

MC 80 DX basic body

456031-0000-000

+

+

+

9.2

MC 80 DX control panel, plus:

456048-0000-000

+

+

+

9.2.1

Mains cable with European plug

380137-6750-000

+

+

+

9.3

35 mm Mot DX film cassette

456071-0000-000

+

+

+

9.4

Adapter 60 for microscope camera, d = 30mm

456006-0000-000

+

+

+

9.5

Projection lens P 2.5× for MC 80/MC 80 DX

456021-0000-000

+

+

+

9.6

Eyepiece E-PL 10× /20 Br. foc.

444232-9902-000

+

+

+

9.7

Photo reticle MC 2.5× /d = 26 mm

454075-0000-000

+

+

+

9.8

Databack D4 (for 35 mm Mot DX film cassette)

456073-0000-000

+

+

+

1035-mm SLR camera

CONTAX 167 MT, consisting of:

10.1

T2 2.5× SLR camera adapter

456005-0000-000

+

+

+

10.2

T2 adapter for CONTAX (CONTAX bayonet)

416010-0000-000

+

+

+

10.3

SLR-camera housing CONTAX 167 MT

416181-0000-000

+

+

+

10.4

Cable release

416167-0000-000

+

+

+

10.5

Photo reticle MC 2.5× /d = 26 mm

454075-0000-000

+

+

+

11.1Other T2 adapters (not shown)

 

T2-Adapter for OLYMPUS OM (OM bayonet)

416002-0000-000

+

+

+

11.2

T2-Adapter for MINOLTA (SR bayonet)

416003-0000-000

+

+

+

11.3

T2-Adapter for CANON (FD bayonet)

416004-0000-000

+

+

+

11.4

T2-Adapter for NIKON F (F bayonet)

416009-0000-000

+

+

+

11.5

T2-Adapter for PENTAX (KA bayonet)

416011-0000-000

+

+

+

! unit operable in combination with microscope equipment

+functions in combination with a further accessory unit

B 40-015 e 06/99

1-11

Carl Zeiss

Axiolab A

 

 

1.4Function Elements

(see Fig. 1-5, after this table)

Item

Designation

Purpose/description

 

 

 

1

Stand

Module base of the Axiolab A microscope

2

6 V, 30 W reflected-light

Reflected-light illuminator with 6 V, 30 W halogen lamp

 

halogen illuminator

 

3

Reflected-light illuminator H

Carrier element for the binocular tube and the illuminator; also used to

 

 

accommodate various technique sliders

4

Camera port

Enables (after removal of the dust cap) the connection of various

 

 

adapters for attachment of photomicrography/videomicroscopy

 

 

equipment (35 mm cameras, video cameras)

5

Binocular tube

Binocular viewing tube permanently attached to the reflected-light

 

 

illuminator H; can be set to the user's individual interpupillary distance;

 

 

after removal of the dust caps, dia. 30 mm eyepieces - one of which is

 

 

adjustable (foc) - can be inserted.

6, 7

Eyepiece, right and left

Fixed and adjustable (foc) eyepiece; the fixed eyepiece must be

 

 

assigned to the less myopic eye; two focusing eyepieces are required if

 

 

reticles or photo reticles are used.

8

Dust protection slider

These two dust protection sliders should always remain in the

 

(2 pcs.)

Axiolab A microscope; technique sliders can also be inserted only if

 

 

transmitted-light equipment is available

9

Objective nosepiece

Carrier for the maximum of 5 objectives which are swung into the

 

 

beam path manually

10

Specimen stage, fixed

Stage area: 160 × 140 mm; for the maximum specimen height of

 

 

- 20 mm (stage attached at upper stop) or

 

 

- 45 mm (stage attached at lower stop)

 

 

Two spring clips (supplied) enable specimens attached to a 26 × 76 mm

 

 

slide to be held in position (also see item 31)

11

Coaxial coarse and fine drive, L

Permits the specimen stage to be lifted and lowered within a travel

 

 

range of approx. 20 mm (also see section 1. 6 (7));

 

 

used for specimen focusing; mechanical unit together with item 29

12

Connecting cable for 6 V

Must be connected to the sockets at the instrument rear to allow

 

30 W reflected-light illuminator

operation of the reflected-light illuminator

13

”A/D” illumination converter

Permits the switchover between incident light and transmitted-light

 

 

when instruments with an additional transmitted-light equipment are

 

 

used.

 

 

 

1-12

06/99 B 40-015 e

Axiolab A

Carl Zeiss

 

 

Item

Designation

Purpose/description

 

 

 

14

Fuse holder

Holder for two fuse inserts

15

Fuse insert

T 0.8 A; 250 V; 5 × 20 mm

17

Mains cable

To apply the supply voltage

18

Pushrod

Pushrod to change the beam path between

 

 

- observation through the binocular tube (pushrod pushed in)

 

 

- photomicrography/videomicroscopy through camera port

 

 

(pushrod pulled out)

19

Aperture diaphragm

Used to limit the illumination aperture; can be changed via knurled

 

 

wheel

20

Filter slider

Permits the insertion of dia. 32 filters in the illumination beam path; can

 

 

be equipped, for example, with

 

 

- attenuation filter D = 0.06

21

Pushrod for luminous-field

Permits insertion of a perforated plate with 1/3 field size

 

diaphragm (incident light)

 

22

Dust protection slider

Prevents the entry of dust. Can be replaced during operation with the

 

 

polarization slider P (item 23)

23

Polarization slider P

Permits observation of the specimen in polarized light (EAST-WEST

 

 

oscillation direction)

24

Dust protection slider

Prevents the entry of dust. Can be replaced during operation with the

 

(2 pcs.)

analyzer slider A and the λ -sub compensator slider

25

Analyzer slider A,

Enables polarization contrast in combination with the polarizer slider P

 

rotary

(NORTH-SOUTH oscillation direction with ± 10° fine adjustment

 

 

possibility)

26

Compensator slider

In combination with the polarizer and analyzer slider, this slider permits

 

λ -sub

the color contrast of anisotropic specimen details

 

 

 

B 40-015 e 06/99

1-13

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