Zeiss Axiostar User Manual

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Knowl€dg€ ol rhis manual i5

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sale ooeration .f rhP inrtrument

The speciiical ons are subjecr ro change;ihe manualh not covered by a. lpdateseoice.
@ un ers expre$V aurhorized, loMardinq and duplcation of this document, and the utilzation and

fam 6 srh r'e

communication
Al n9hr5 .esery€d

ol

conlens ol

lts conrents are noi

in the ewnl ol

red lor rhe op€ration ol the instrumenr. Tnerefore

rcq!

lls Tarualard oav

permited

glanl

n9 ol

patnrs

or rcgGrrarion ol a L{liV model.

soecialalle

voariont wll email a. oblgatio. ro

iror o hnts.o'.qnT

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Phone: ++49 551 5060 650

T€l€laxr ++49 551 5060464

l.ternetr
E mailr [e-mail]

Nufrbdr oi thls franla : B 40 031 e

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CONTENTS

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1

2
21

2.7

Genera
Notsone{ety

ard opecl on
Nores on efery: care aid ma dena

DE5CRtPTtON,..,,.,,.,,.......-........

Name and ntended appli.ahon
De(ioron aid na n

Eyspe.er.. .... ... .. ... .. . .

5iaqe
re.hnc

Ana.hmem
Ara.hment Dt bno.u r tube 45"/20

ar..hmenr

kraw iq n oi obreclvei .. .. ...

nseit'onof
nsefrion

Compenrt.nolamd,opiawheneyep,e.p,eti.sreusd.......

Ata.hnenr oi ldd iq eye.upr ..

setinqor

Ata.hmenl ol3pernen horder to mo.h.i.alnaqe
Ana.hmenl
Anadrmenr olAbbe.ondenser 0 9/ ..

r.jenon
atla.hmedorAbbecondetrerwrh
ara.hhenl 0l LED inunmdor..
AtG.hmentof
conn€dn9 fie n\rumei' Lo,heline

noresonrrely, nnrumenr

uopart'nq tanspo( sto,aqe, d sposal

retres

mcomerer and ey-cp e.€ Et des

d3ra

ol rube.

ol b no(! rphororube

eyepe(e5. ..

ol eyepe.e rct

ite4upillarytlitraf,.eandvewnglreght.... .... ... ....

of.ondene6

oi s der lor

pha..oitst

coored

tlisk.drer

de

..

45'120 .s

drkle d

o,

podzer

75 x

30

o,wde.angle un r.

R

1-3

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... .. ... . 2 3

,. - ..... 2

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Usnqbendnsmred
rrammiued.

Ser'iqorransmred

lsinqtanrmted

T,ansm(ed liqhi

setinq oI trnsmted

(he

Lrs

dg

Cenera

TGBmted-]9h1 darkled.oilgtrr

serng ol ransmitod'light drkled .....

nq

Us

tansmfied

roismted

nq ol tan:mited lqhr

5et
sampe d lfa,€n!{oi bdw.en

Ararhme^r
Ana(hmed ol
A|a.hmeftolslRcameE,e q co
ArachmenrofMc 30 Dx m(ros.ope .amec
Arlachmenl

Aib.hnenrol a&pte^ lordgrt.ompad.ame,as .... .. ...

Diqita .ameraswth 37

DiqicGm€,aswth52mm1ie,th,ead..... ....... ... .. ...

Atra.hm.niol omer3sw th orhe, r rer th.eid rzes ..
tony otc 97oondiqG came,aada

tht

ransflnred-l9ht darlled re.hnique

p'ncpe

tht

iI

.5 doubre rubo ...

phorom

ol adapteElorvdeo came,a3

qhlblqhrrieldle.hnque

bi9hrlield .onnqu

qh1

b'qhrrieldlo,

qlrtphae.oita

pbase.ont.4t.onlqucton

qht phase.oitan

pobrnaiion

'qht

pol.rzalon

conl,a

po

rzd

qour

c,oq,aphy equ'pmenr

mm1

t, th,ead

KdHLER

.ontan

pseudoqout

aid

{19

umindon

mm rilm

€$ette)

...-- - ,

.... ... 313

.. .... ...3l4

... ..

.. . ..3a7

... .... . 3

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CARE. MAINTENANCE AND TROUBLE5HOOTIN6

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Iabe of sparE, wer nq

ANNEX

oJ AbbPviaton5

Lisr

Phys ca andTe.hn GlUn ts.....

Certf.a1oi

l"r

qnql|r6v

in A(ordan.e

.ej

70w \aoqq arp

pads

3id rools

w$ DrN lN 50 9001/D

N EN 46m1

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G€nera notes, instrument

salety and EMC

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General

B€lore nanng-up lhe microcop€
ensure
lhoroughly

F!.d€r infomaron
seryice depanment or lrom authorized

The Axionar m.ros.ope is an oprical

nntuhent which may be impaned n its

perforhan.e
Th€ m c.os.ope mu5t be

pe6onne

possible

To e.sure rhal the working
and thal the nicroscope iunctions whhout

probems,
the operal ng in*tudlons nun be obrerued

rhese

ll

the saiety notes are not obie ed there

not€s on safeiy

p

ease

you

da.ger

are highlighled n the terr by the

have lamiaized youBei

w th the conten$ ol th 5 manual.

can be obtained lrom our

pre.sion

or

damaqed

operaled

who mun

only

lnvolwd wirh micro5copy

notes

the

and wa.nngs

I handed

by

tralned

be aware oi the

and

pro(edurc

is saie

incllded

n

llot surfa.€, rh€r€ is. risL of burns.

lI the rafety notes a.e

not

obsetoed lhere is a

tuk that rhe insrrument wil be denroyed.

6 NOTEI
Noies which must be obseded whe. working

Initrument Sa{€ty and EMC

The Ar ostd m croscop€

produced

61010 1
ele.trkal n$utihg, contrcl and
tnsr.urer6". t leit the

The A{iostar mcroscope meet the

rcqunementr ol lhe EC d rectives 73l23lEC­app€ndh 1 and 39/316/EC and fie FMC

egislation ot 0911

Rado

and teited

(lEC

l0l0-r),,safety

interference

i.

comp

1992:
s slppre$ed

wa5

ance wlih DIN EN

requieme,tr tor

rac(ory in

designed,

taba.atory

a

in ac.o.dane

pe.fedy

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CAUTLON!

Dk.onnect the innrumenllrom

th€

tz00

ine

Resiiant to
50082 2

conforfrity with the above EC
documenredbytheCEsymbo.

tamminq

in accordan.e wih EN

drecrw5 is

unpackinq, Transport, 5toraqe, operarcn

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,

Unpa(kin9,

Pease obse e the lo owifg saiery nores whe.
unpack.q lranspod ng a.d

li

a.cordance wth standaid

mdo(ope s suppred . a

with .adboard

or9 nalpa.kaqing tor transport

Relan ihe

perods

-

When unpacknq use the deLiv€ry nole to

ensure $at al conflslratio.s and modules

transport,

pa.[agfq

pa.kagng

relurn

or

lor lo.qer

of the

stora9e

norn9 the

p.acti.e

panlc.onrainer

ony

!5e

norage

iffumenl

ro rhe

rhe
lhe

m

Obserue lransport and norage

temperalur€s

Pace ihe m

in a..ordan.€ with

c@scope

on a

nable

the

wod

io lowinq salery noles

/i\

lhe m

The nlcrorcope
a
groundnq

mun not be made
ertens

ff

urer are

-

-

-conract an aulhorzed workshop or the

(o(ope.an

propery

on .abe wh.h doer nor have a

elrabkhed rhar the

I 5

swtch
il€glard

manlfadurer ro lrave repa 6.arfed out.

nsra

contact; the

no onger en€crvel

ofl rhe mi.roscope

againsi nadvefteni operation

be op€rar€d wiih a iie

must ony be.onnec(ed

led

socket

rnefiedive

learurlng

yolnd.g

proledion

when

to

a

elfed

by an

meas

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G
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3
G

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Keep oprca surface5

When dspornq ot ihe Axiostar mlcro5cope
p

ease observe the

Def€cl ve m.ros.opes do nol belong w

they sho! d be disposedor

pa.[aq
rerevanr

f in dolbt

nq and n accordance

rarubry

io!ownq

please

lree

provE

ons.

(o.rac.

linger

of

saJery notes:

in

piic

th

@AA

Aways d sconne.t rh€ nnrume lrom the

ne before

.hang nq the amps or

only use llses ior the

Ihe u5e of

. r.uiing of rhe

Be .arefu oi
wh€n usnq the LED

rearly into the i umna1or wilh

d
eye5 irom a
are rcqured, the
a..ording y va the

openng lh€ nntument,

.hanginq lhe luses!

requn€d rated

mak€shi{r fuses and the short-

luse ho de6 are

lght-emlt nq d ode rad

lumnaior. Do nol

your

short

disian.e

nrensly must be redu.ed

potent

when adtunmena

ometer,

or a surtab

not

aron

loo[

naked

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Care and maintenan.e

aare and Ma.renan.e, Waranty

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eas-a observe the lo owinq saiety notes Nhen

.arn! tor and manlannq the Aronnr

ft,/N

Wth the ex.e4on of the work spe.iled I
ahaptet I a1 th s manua , CdF,
naintenan.e .ntl TtaLbl6haotin9", na
manlenan.eor repair may be
the us€r. We axprosy
orher repa6 mun
.ur aurnor Te0

Damaqed nntumen$

.ny be

and
fie

It

the

usnq fie dust.ov€r; make rure that t ha5

repaned

no6rure,

perfonan.e

nicroscope

o.y be caiied out by

pe6onne

o, .ompoienE mun

aid oa nla ned by our

these ntuen.es..n mpar

ol the inslrum€nl.

s nol rn use,

pedormed

point

out that any

aqa nn d rt, dun

pror€.t

by

il

AA@

Lam$ are lo be .hang€d rn a..ordan.e
wnh fie neps n chaptet 1
M.intenan.e and TrcLbl4ha.ting

Ds.on.e.t the Innrument tom

,,carc,

the

Ine

The Axionar m
a..e$or es, may ofy be us€d tor the

m.ros.ope t€.hniques de(ribed n ihs
manual Th€ manutaarurer .annoi asum€ any

ab lty ior any other app i.at ons

Pea5e obsetop the lolow ng lvaranty noies tor

The manlfadurpr

nstlnenl has no material and

deiects when de

Yo! mun 'nform u5 ol any del€.ts
imdedar€ly
min mize the damage

I the manuiadurer 5
deie.t h€ is.b
de.son wh€ther he doer th6 by
ihe innrlm€nr or by de

nnrumentlree oi any defeci.

-

guarantee s prov'ded for

No

by natu.a wear

The manufa.rlrer s nol abe
.ausod by lauty operalon, neg'gence ar
ant orher
paftcular

excnangng n froscope
u5e ol accesor e5

unaurh.rzed nleNe.ron

.laims aga nn fie waranly

(os.ope,

ivered

aid do everylhng

ged

(wearn!

tamper

a rpsLt ot removng and

a5

nruding ils origina

luaranlees

infomed

remove

10

part in

ng wth lhe Innrumeni,

.omponenE or rne

prod!.iion

po<ibb

i:il s

vern9 a.

defeds

lor

tom orher

'nva

lhat lh€

ro

of su.h

his

repainq

caLsad

(ular)

Da

damaqe

dates a

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L€i ihe amps .ool

rcep the amps iree oflnqer

down before changng

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co.tents, Ln oI I ustrations

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INSTRUMENT

DESCRIPTION

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INSTRUMENT DESCRIPTION

1.r

The Axiostar
lorlhe visua

n b o ogy

aboralory m.ro5copy,

-

rra n n9

Name.nd iotend€d

5 a ranshifled ltqht m

zation oi ine nru.tur€s and lorfis

and med cne Typi..l app icat

(schoos

application

and unlve6tier.

(oscope

ons oi

1,2

Thanks

compact lransmirled-l
In additon to high rBolur on c5 objecdves

rh€ major bnghtiield, darkli€ d,

and
oplional .amera
documentalion 6 aso avaiabl€ lo

Major

-

tiurdyand convenentnand nrh€

userlrend
MechanicaL itage 75 x 30 R wrh cerami.

.oated nagesu ace and specnen hoder

-

spa.ejavinq and .onl nuously adjlsrabb
inieEared lumnaiorwih lonq fe6v,

D€sc'iption and main featu'e5

pyramid

its

to

poarizalion

nsfi ument

y.oax

des

tht

.onl.an t€.hniques,

port

lo.

rearures:

a .oaEe and line

the Axonar is a

9n,

micros.ope.

pha5e

pholo

and

contran

and video

the user.

pyfam

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7 fhe
s aoaer&u'igdnc(tuo.w4)

r3
rs ceder nq

roas iq d,i€

kwr rof adjunhq:p€due

(rew

(Mw:y)

d4tr4m

for.ondenar

lrM

way)

0 9/r 25
and

Bal bearifg,4 or s

ICS obl€.t v€s n the
caregol es CP.Achromal,
Achrop an n iiiey

(onde.serrof

phaie

contan

3 nocular tube 4s'l20

height ot 425 - 470 mm and bifocuar
pholorube

100% v5l 100% do.
l0xeyepiecesrorlhetieldfumbeE20or

18, suitab e ior eyega$ weare6, ixed and

45',20 c5 wrrh lo d nq

b.qhtre

Ph r,

2, 3

postion

pr.e/performan.e

A-P an

qradualed

rcs

wirh a vewinq

d, darkfeld

nosepie.e

and

rows

prsm

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donaan ia,r 5i!

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dano.o6:iGij:iii
iliir-i:ddoddiro

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(rb

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;laransoii:d;ii'

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rhe

oble(tives areiheopica centerpie.e oJ

The io owinq is an €xamp e of how obled ver

cP-acHRoMAT

10x = oblecive fragniicalon,

.oLor rnq on lhe obj€.tive

beinq alo.aled io ea.h maqnlication

nep

0.25= numencalapefture

-=

0

Oil= oi mmeEion objective
Ph 2

intiniie tub€ enqih

=

aan
O=Oor0.l7mm

17 = .an be us€d wih.over 5lp lh.kne$

- phase.oniran
objecr ve

t0r0 25

(car

ze s.olor.ode)

be

usedwlh cov€rnipfil.kne$

labeln9 and ann! ar

-/-

with

obje.rive wrh

a delned

qreen

F

The object've

objectver may only be
iearurinq an lniinire ube
innrufrenrswhose obje.tiv€s

'160

The
ol 0.r7

h

objecive.

objectves.an be set
th.kne$es via a cotreario.
speclmen ar€a s 3€arched, and
rhe.otrecrion ri.q where optimum
lmage.onirasi are oblaned

(relocusinq

mme6ion obie(ves

diiferences n cover i

when imme6io. obiectives

betw€en the.over slp and
rep a.€d with a
in mon.ases. The
20

Due ro their shod
25x and hgher

.s rhe r me.han ca lube

exaar

qher

m ol 531 N mme6o 5

labe L.q

obseruan.e oi ihe .orer 5 p th ckne$

mm s a rh€ more nece$ary

nw€r.a apenure ol rhe

the

rhqelor€, so.alled Coii"

c a ways requned)

-

ndicat€s lhatihere

Lsed wth mi.ros.opes

engih and nol wlh

arc marked wrh

length

lor dflereni.ovd sip

rinq. For ths, a

rhe

is determned

ar€ always

p

thickne$.

quid, whch is mmeEron ol

pLasrc

work ng dnance, obl€cuv€s

lealure r€s ieft

nsenllv€ to

are used, ihe ar

rhe objectve s

oler.o.iainng

particuady

rhe

posrlon

1o.us and

oi

mounls

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Th€ objectve magnllicarion muripied by the

e(e magnili.arion

eyep
the v sual overa

The num€r.a aperrure x 1000,
e.9.0 25

usefulmagnifcation,
reso ved above thal

I maqn ficationr

x 1000 = 250r, i5lhe highen

(usualy

e no furthe. detais are

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The

io owi.q

oblecl ves are

avaiable ior ihe A\

oslar mioos.upe:

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nnrumenr

Eyepe.es Slae-e mL.romere6, Eyep

de5cnpr on

e.e

retr

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a,
a
a:

The fo

i"q

1.6 Stage mi.rometeB and eyepie.e reti.les

Mearur.q
se€croi of whL.h s ned beow

ow'iq eyep ece5 are ofl€rcd Jor lhe Axonar

Etepele PL l0nl3 3, fo. 39"

41"

Et€p e.a E.PL 10J20 B fol 444212 9902 000

Ey€p e.e PL 16116 3r fo.

L"

'"d"r"

a.d.ountrq u3 n9 the ml..os.ope

D o

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Descripton,
stage mi.romeier,

'lradaton oi

I mm 'i

rb"od""d 'o- " \.

requ

r€s nnq€

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positive

+y.ar

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Crossline mi.rometer dirk 14:140
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qranaton

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1o eran.e < 0001 mm

=

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mm

26

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Crosslined'sl/d=26mm

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croslih€ mi(ometer diik
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12.5x12.5/5;10/d = 25 mh

area

Photo reti<le

lor 35mm

magnil
photography

length = r0
toerance<000T mm

l2

5x 12.5 mm, d vid€d in ie dr ol5 x

MC 2,5x / d = 25 mm

photography

cation of 2.5, or for aqejomat

wirh a l0x addnona

mm

wirh an add ional

10100

474066-9901-000

474068.0000.000

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B 40-031 e l2100

lI an ey€piEc€ retic e is

lo.. €yep ec€s .ontaning an adlustable

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used,lhe b

pointerlor

poinl

towad objecrs n ihe specmen

fo.u3ins €yepie.e /

nocuLa.

tube or

eyeens,

000000.1095-418

l-9

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(1)

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OveGlheighlin.udinq12 adaprerand CONTAXARIAsLR

Axionarwrh b nocuar

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5torage and transport

Dp
P€rm$berelatvehlmdily(wtholtcoidenfion)

Perm$beambentlemperatlre
Perm$ b e ielatve

Akpre$ure

Dihensions

phorotube

Ambient .ondiiionr

r

bF or 04

.. ..

(widlh

'en p.rdr.ts

humdily

rdeplh . height)

...

tube ..

(in

pa(kasins):

(wthou1

condensaiion).. .......md35%

.........approx 200 ! l50x45O

camera housng..........approx

appro,200 150.450fim

mm

57Omm

approx.6.7k9

. .. .. 40 o r

max r00,i

.........+10to+35'C

hPato 1060 hPa

800

/0.C

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Co.veE on of the

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Adjunmeni ol

io|rpe

safety. . .if.omp iance wth D N

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100

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averaqe re ............

(7)
5tand

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5pe.mennale .. .....nerhanicanagoT5rl0Rwth.eramcsurface

spe.menhoder. .. ..wthsprlng.iptotherqhtloron€handedoperaion

Abbe.ondenser0 9/1.25. . . . . .

optome.hanicaldara

wth

wilh ield.umb€r 13 . . . . . . PL l0'J13

wnhleld number 20 .... E

Dmenslons

T.ave Enqe

ma^ihum

nage

locu5 n!

(width

(wdlh

fed

nlmbe,

xdeprh)

x

depth)

. wilh.oa6e drivei(4 hm/U)

. . . and fiie drve

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. ......... cs iie ol.bj€.ivei

PLl0t20Br andE PL10V20Br to.

..

lorV!! <4iwth ude anqe f!n.ton

.an be sel betwe€n

(0

4mm/rJ)

.v€ral lft l9 mm

yva4l5-po5ton nosepi€.e

wnhW03 thread

Br and PL 10113 Br. to.. or

.. . . . . . . .. .160 x 140

... .75x30mm

4-'15312

' ',','' 20

55 and 75 mm

mm

000

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Binocular

phorotLbe

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vewing anq e

viewlng he
v6ua
Gmeravo€o

.ameravoeo

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45'l20

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potr

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START-UP

2

22
221

2.2.2

2.1

2.4

2.4.2

2.5

2.6

2.1

2.1.1

2.1.2

2.1.3

2a

29

Anachme.r of binocuar

screw

nq

in oioblecrver... .........

Contents, List o1

phororube

45'120

l lunraton5

c5 2-2

2-3

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Fig.2-1
Fig.2-2
Fig.2 3
Fiq. 2 4
Fiq. 2 5
Fig 26

Fig

29

Bid 2-13
sid 2-14

artachm€n1 ol brnoc! ar

setlinq of inrerpup llary d nan@

seting olviewinq heighr .......

Afiachmenrolspe.imenhoderlomechanicalsta9e75x30R......,..................2-6

Atachmenro1Abbecondenser0.911.25................................................21

nseronolsderlorphaseconlrastordarklipld.............................................2-j

Atta.hmentolAbbe.ondenserwth5-posltiontlrei..................................2-8

AitachmentoflED iLuminator...

connectionoILEDilumnatortopower$urce........................,...................2-10

atlachmentolcoofeddirk6(er,poarizerorwideangle!nit............,.............211

connecdng the i.sttument to the

lube/phototube

2-5

......................................2-5

.. . . .. ..... ..... ... ........ 2-9

ife

o

Unpacknq Atlachmenl oi Tube5

2 START.UP

!€
Belor€ innalinq and nartinq up rhe

.arel! ly read through and obsetoe

'Io

prev€nl

suda.es

when unpa.[in9

inqer

pr

nls, do nol to!.h op1.a

2.1 Unpa(kingthe

'Ihe

Arionar m.ros.op€, n. udinq a..e$or€s,

r deLv€red . nandard

.

Remove

.are a.d

.

Kepp the
.an be st.rad for a longer
returned

one kiurled 3crew and
ea.h afe slppied for the bino.uar

(pholorube)
.an b€ us€d to .amp ihe tube

.

S(ew ihe req!red

hole12-l/6)in rhe srand

2.2.1

the hicro<ope

pla.e

t oi thewor( tabe

pa.kaqnq

to the

45'120 cs Any of the5e 5.rews

Attachmebt

manufa.rurer

inerrufrent

pa.kaqnq

trom

rhe iran5pon

so lhal lh€ nnrumenl

p€rod

one he$qonal scew

(rew

inlo the threaded

of binoolar tube

ol llme or

(Dhototube)

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Fig',JAfuchnentofhhdfu]aI|ubJPha|bfuhe

.

Remove dun .ove6
lnde6de and lhe dovetai
nand by uns.r€wnq the

(2'115)or

r

Hod

nc .ed
nand mounr vla the doveta' aid lurn
the r€quired observano.

both rhe
backward.olalon are

t

Tqhten the knur€d

hexagona s.@w(2-114)

2,2.2

!

Remove

s..ew

.

Ara.h rhe
rhe nand mount n rh€ same way as

.

rqhten

th€ hexaqona screw

the bnocul& tube

portof

Attachneot of

dln caps

(2

16)or hexagonal5crew 12

(nured

to the eft, atta.h

lustated

binocula.

5.rew

{?-rl1)

mount on rhe
knuned s.rew

(2

(2.1/2)

roraion

po$

(rew

(2-1ll);

phototube

C

and lhe 130"

b e

Q

bino.ular

oosen

r/t or hexagofa

lrom rube

r/4)rrom

n a sighly

I to the

it to

pollronl

r/5) or rh€

knlrled
1/4).

(2-rB)

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Remove duncaps
numbe
(2-26,

by one. rranio 4il' Lhe
maqnr.aton.

ne

nosepiec€

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if

rcrewhgin or ooFdrves

(2-212)

o'objedires -d r!{ ob_eatres

ii@ nosepi*e

oln caps snouE

eyes wh ch a.e not requ red,

\2.2,1,

rnsertion

accord n9 to rhe

cloct$ se o-e

ot objectives and eyepieces

lw4.

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Fyep,Re

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In*rtion

P, 10V 8 Br fo..

rymr(e

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rube ard tle'dusi.q

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840-031€ 12/00

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2.4_2 Conpensation

when eyeFie.e

The

coiiecl use

locueng

two

Br io.., to fiale
po$be

.

.

When the

n lo.us n lhis eyepe.e,
th€ se.ond ey-a

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usnq the

io.usnq drive on

ametop a ol lhe

LJse

th€

locu5 on the lne igure oi $e eyep ece

ro

Fo.us on the niaos.ope
spe.imen va $e foclsing dave
through lh€ ey€Piee wth

,maqe h rocus€d lor rh€ s€.ond

ot an ey€pi€.e reti. e requres

eyepe.€s,

po$

il

ey€ens oi $e iocurnq eyepece

male and the eyepiece relrle

via

the

fo.ueig

eyelens,

€.

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obseruer s eyes

to.us the Lmaqe

lo.usinq eyeens oi

the

of anetropia

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retle

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PL 10r18

mage ol a

by iooknq

pos

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2.4.1 Intertion

The

eyepie.es

E PL l0x/20 Br ioc are
The s ilhl imaqe shift.aused by the addrion,

paih

ihrolgh rhe

on $e d opler 5cae
polnt po5

(2-4^,v)blt

dot
The ey€pie.e d ces
ariached to s.r€w

r

uns.rew

.

s.rew n rhe new

req! red eyep e.e retc e.

s indi.ated nol by lhe whle

tion

rh€ mouft ronianing rh€

6f eyepie.e 'erkle

PL 10x/13 3r 10. and

nlended tor

qla$

is taken into a..ounr

by lh€ iacr lhat rhe zero

by the

red dot

n mounrs

mount

(2.4/R)

(2.4/3)

(2 !t/4)ro

containing $e

lse w th

have

been

alow

Att.rhment of

The €yepeces are eq!

proiear

to

These

.

Remov€ rhe rLbber

pyep

somermes,

very tiqhty n rhe eyepi€.e

dulobFct(€! iooth

lhe spectacles

rngs

e.es andatach lheeye.ups

the specrace

be rep aced

can

pnk)6

Ioldinq eyeops

pped

wlh rlbber r nqs

tnq5

prolecton

lrom s.ratcher.

wth fodnq

(2

4/2) fiom rhe

(2

4/1).

rinqs nt

groove

r-pqur€d to

so lrrai

a

Fre$

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rn.

idvd.a interpupiltr
obsele br swinoro
svmmelfca lv towards

.

The vi€w ng height is

r€quremena ol

swnging ih€
(2-614)

s6ttlnE ol inrerpupittdry
distan@

dyepiece

of

disra..e . matfed

eyepiec€ rubes

downwards

rnierpupilary

andvaewins heighr

lo

.he eyepele uo*

o.e a.orher l2-5)

frat hed

the ndvduat

(2

6/8).

to the
lser b!
upwards

distance, Vising height

rr

Flg-2.55djn9ofidgeuPi||!4ldkcnc

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840-0Jre

12/00

2-5

Ite.ha.

ca sidqe,

Spe.men hotder

Atra.hment
to m€.hanicalstage

the

axiostar mtoscope

me.hanca

The

ipe.imen s

nq

us

the.oaiia drve

Th€

/y

wfth lhe

The readly
rprng
of the nandard.onlig!ratio.

As an ate.nalve
.

the spe.imen holder wth
(473443

the
operaton

Here, rh€ miooscope

ns€rled nro

gudnq
and wl aLlomatca y

The
ex.hanled n

lixrng s.rews

Loos€n the
spe.imen holder
spe.men holder
qu

d ng

staqe 75 I l0 R wth

pre.sey

gradaiion

lwo vernier 5.ae5 hep5

.ip R

spec men ho d€rlor one-harded

edge,

spe.rman holdeE

5loB. Tiqhten th€ rwo fx nq s.r€w5

on the nage sudace

mounr€d rp€c'men

(453536-0000-000) (2

ilis aso

0000,000)

(451543'0000.000)

the rpecimen holder along

lhe rprnq .ip s 5wLn9

the r drecl@n uinq th€ two

o. the

two

of spe(imen

nand Jealur€s

moved

(2-714)

po$be

(2-7l2)

c ahp fi€ m.roscope

qu

d nq ral

Jing

to the

{ronl

unril il enqaqes n rhe

holder

75 x30

.eramic

ln x

to reo.at€

hod€r wtlh

7/1) is

to use

5p.ing clp R

(2

7/3).

sl de

screws

12717) s

(an

b€ easly

pu

and ns€rtnew

R

and

(2

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pan

lhe

our

out

the

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2,7
2,7.1

.

Urscrew
the
condetuer 09/1.25
inserted

.

Insert the condenser
.arief n

I the dovetaloi the.o.denser

2 the or€ntatnn

t

lghten

the condenser .nrier
engage Ln tfre dovera I
condenrer

Att.dment
Atta.hment ot

both centernS scrcws

condenss ca(er

nto th-a .ondenser

such a

againn lhe 5prnq
cond€nser cari€f and

und€tride

qroove

of ihe condenser €nte6 the

(2.8/4)

rrolrr centennq s.r€ws

(2

3t) in

ot condensere

Abbe condens€r

(2

a/l) unn

(2

3')

into ihe cond€nser

way

that

pn

5crew

on rhe condenser .atrrer

pos

lion

p'a/l)

(2.9/3)

ihe Abbe

can be easiy

canier

s

{?-8/2)

(2

a/5) of the

(2-813)

until $ey

and keep lhe

on

pre$ed

i lhe

on

a

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a

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2.7_2
!

hsen the dder
the openinq in lhe

upwa.ds and €ngage
12-9t2)

Insertion or

5lider for

(2

9/3) unri

condenser

9/4) mun la.e

{2

n $€

pha3e

n engaq$ in

(2

9/1)i the

gudinq

sot

a

i

il

2.7

,.

2,7.3 Atrachment

Alia(hmenr is mad€ in the same way as $at oi
the Abbe condenser

Loosen bolh cent€rnq

rhe condenser cari€r

(onden5€r

Press

(2-1012)

ipr.9

Make su@ thar
(2

groove

pn

r0/5) on th€

(2-l0l4)

of abbe.ondenser

o 9/1.25.

s.rews

(2.10/1).

with dovetail agann rhe

n the condenser carier.
rhe orentarion screw

.ondenser enqaqes h the

or lrre.ondenser

(2.10/3)

calrer.

on

Q

6
t

6

o

g-1,oAfu.hn?ntofAhbddnd.6qwi|h

Tighten bofi .ente n9
the condenser.aiier
enqage
condenser

n the doveiai and hold rhe

(2'r

0/7) in

(reM (2-1013)

(2-r0l1)

ion.

Pos

on

unll they

a

5
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a,

fr

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2.7.4 An.chnenr

.

nsert
condenser.an€r
as the abbe .ondenser

.

Tighlen
rhe LED i um nator er approx matey n ihe
cenler ot rhe beam

'

Use.ondenss d.ive

.ondenser.ader nto the topmo5t

Power
ehhq via the llne
oi rhe
Akaine batery
the nlelrared banery comparlmenr, or via an
€xlerna voiaqe sour.e ranq n! trom 11 10

14 V DC

voltage sour.e mun

ot up 1o approi. 120 mA to be oaded.
The

LEo iumnator
neltal white
temperature ndepende oi the set biqhme$
rr

5 sutabe ior objecrives ranqnq ion 2.5xro

100x. The lqhr emttinq diodes hav€ a onglle

anc ca6e ony frrnor operannq cons

LED llumnator

bo$ centernS <rews

to the LFo

Ine,

va a cofrme(a y avalabe 9V

(9V/N

(e.q

12 V .ar ballery) The ex1€rnal

lght

of IED il'uminator

(2

1rl3) in the

(2

11/'l)in the same way

(2

rrlaunti

palh

(2'1114)1.

llumnator

power

permt

provdes peasant,

wth a constant .oor

can be suppled

unt or, independent

cd batiery bo.k) n

a .onnant.urent

move rhe

positon

color

a

a

a

I
I

a

a

a

I

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I

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operation with

.

ns€rl .ountry spe.ti. adaplef

inlo

lhe

r

.sert aiq€d ronnedor or the

(2'129)
the LED lum naror

'

Connect

The operaton ii€ with batlery operalion
nrongly dependi on the s€t briqhh€s and lhe

oading capac!, ol the badery.

power

power

inro the +12 V soclei

power

unit

unLt

12-l2l9)

(2'12l1)

uni to the

ine

\2.12/1Ol

1212) ol

\2

B€.areful oi lqhtem'ttn9 dode

when using the LED luminabr. Do not Look

recny .b

d

lrom

eye5
a.e requred, tha
accord

a short

ngly

v a the

lumnaror wilh

rhe

distame. When adju(ments

ntens ty mun be redu.€d

potenl

omeler,

radalon

your

naked

or a sulable

a

Operaioh with 9v
(9v/

Ni cd

bartery blo(t)

Alkaline

)<,

1Z

:r

9

Ium holding

(2-lr)
iett

and rcmove rhe.over.

lnsen SV Alkalin,o
ban€ry

reminas

Atla.h cover,

pos

ijon by rurning

opetation with external voltage5upply
.

lnse.t anged
adapler

5ocket(2 l2)of rhe LED lluminator

t

Connect cable .lamps ol

adafler cabe

red clamp to
bla.k.lamp

sfiew

ot rhe barery

compartne.t

i. lront

con.edoroifie 12 V battery-

cabe

(2-lzil)

plur pole

to minur

(2-1213)

compartmenr ro

batery

(2

(pus

pre$

{2

it

holding

rhe

r2111) rnlo

(1),

pole (").

of ihe cov€r

(21216)into

12ls) wrh

pole points

on and iix it

sdew ro

+12V

the

12

the

to the used 12V

V baire.y-

the

the
rhe

in

rhe

I r2vbarer3daprerabe(4hronq)

Flg2n,cannedion.lLEDunk*t|oeo@|

Depend nq

(2-12l3),
guaraniees

s sufiicient loading

fie loadng .apaci9 is

luminalor wi

'

After .onneclon
swrt.h

toggle switch

.

Match ihe iiuminaton intensiv

on the bl

rhe inteqrared

.onsrant br

the LEo

qhr.6s

ghtne$

capa. ty

go

oui or drop to minimum

10 ihe votage source,

lumnalor

(2-

1 z4)

slre to avoid ntense

12 V batery adaprer cable

sei va

curent conrro

as

lrom

exhauned, the LED

on or ofi via rhe

the codro

lonq

a5 th-aE

ihe battery.

via the

rf

t

2-10

840 031 € l2l00

a

t
t

Co ored d sk

5tart-up

(atrer,

polai?er,

wde anqle un t

t

a

t

a

a

t

a

I

a

i

t

a

a
a
a

I

t

Afta.hment of
erier,

(olored

Ho

d

the unde6ide oJ the .ondenss
(2

l3ll) and
.olored disk .adq
thread on rh€ botiom
.arer
ofls€t hebgona key

Screw nop bok

12-13/4)
.ondenser catr er

S(s wdeange unt or *nqin
{?-r3A)
{2-13/3,

hoe

por

unt

unrilrhe lhreaded hole olrhe hoLdnq angLe
{2

$readed hoe ol the condenser.amer

.ontads rh€ undeEide ol rhe .o.densei

(2-13li,

wth

posiion

oJ the condenser carer

tlon a) by lurni.g rhe ent re wide'ang

(o.

the

l3n,

posilon

polarizei

dik carrer

(rew

po5non

(2

nro ihe rear rhr€ad on

(2

exceeding lhreaded bolt

a) nlo

polarzer)

(olored

or wide-angl€

(2

the holding

(2.13/6)

elt of the condenser

a) usng rh€ 90.

5W 2

13/3) wlh adjuning

posn

1ll1,

the l.ont rhr€ad€d

5.rew in rh€ lnt

b) ies below the rear

dirk

pa.aLlel

I l/2)

bo ts ol ihe

nto

ihe iionl

(2

13/5) unii il

on b)unliiir

(2-13l1,

to

caiier

ever

ihe

polarizer

e

and

q.

o

I
I
I

I
I
I

a

a

I

a

a

t

a

Then use the dotted
rhe hodinq

(ondenss
ihe suppled notred s(ew

ange to the underde of rhe

caftier

screwdver b s.rew

(2

13/1,

porton

b)with

211

conne<rins the itutrument to

Atr

The

microscope @n

vollagesof

r

connect the llne cable with
(2-r4,?)

and .onnect the eanh-conract

.

swich on the ifn.ument

iwilch

.

The

switch
inst.ument is @ady

' '

100 240 V withoul co.veBio..

io the nsirumenl socket

(2-14la)on

green

p6iton).

LED

i9ht5 Lp to ndicale lhat

be opeGied uing ine

the ei1-hand

inl€oraied

lor operation

Th€ inreqrared

(onnecior

(2-1411)
(2-14/:|)

plug

va the on/oii

side of the

i.to

harogen

odofi

the

th€

(swirch

lamp

in

i,

o,

{,

6,

a'l

ar,

a,

al

F

2-12

3 40 0!l e

l2l00

a
e,

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ai,

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a

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t

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I

OPERATION

3
31

a2

32.1

3.2.2
323

(ansmrr€d

3a

342
333

341
342
343
35
351
)52
353

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la
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342
39

r0

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310.1
3142
3103

311

ng

U5

Ge.eralflnclple .... ........... 16

Transmifted
seitinq oirransmtlled- ight

nq rhe

Us

sefting oi rransmfted- ight darkfield . ... ... . . ........39

Us nq tra.sm tt€dllghr

Generalp.n.
Transmitled- ighr

setinqolrransmited-ighrpolaizaUon.ontran ....... ..... ..... .......3 10

sample dilfer€ntiat

Measurementofengths................. ...................312

Atra.hmentolcsdoubetube...........

Atla.hmentof

Atia.hmentolSLRcame6,e.g.coNTAxARA.....

Attachment
Altachment

altachmentofadapi€E1ordgra.ompacrcameras................................3-19

Dqracameraswth37mmiiterthread .. ........................320

Dqra.ameraswith

Ariachmentofcameraswilhotherfterrhreadsizes......

sonyDsC-SToondqtalcameraadapter4-4Ms2x07s....... ........3-22

Packinq

qhr phase

ransmlled.

qhr

pl€

phoromidoqraphy

ot Mc 30 Dx mcros.ope.ameG

oI adapt€E lorvideo ..................3-r7

the inntumenr . .. .. . .. . ....... .. . .

lqhr phase

qht

darlneld.onl

polarzaton

polaizatio.

on beNeen

52 mmii€rthread.... ................ .

contan

.ontran .onliquranon ..... ..... ........ 3 6

phar

darlf e d re.hn

gurai'on

contan 3t0

contrast conligurar

goutand pseudogout

equ

que

pfreni

.............................310

on ..........3 10

..

.... ..

(f

lfr ca$etie 35 mm)... .... ...,. 316

.. . .. .. .. .. .. .. 31

... ... ..........3 l1

.............313

.. ....3-14

.......1'14

.. ... .....3'21

..

...........3-22

.... ...

l6

la
laTransm'ned

3-23

ia

a

a

I

a

I

a

a

31

Fig3-1
Fig.3-2

Fig. 3-3

t

Fiq.3-7
Fig.3-8
Fig.3-9
Fig.3-10

Fig.312
Fig.3-14

Fig.3-r5
Fig.3-16

Setting of transmitted-liqht

'DigiE

A-a.'r.n' ol

Attachment

nsen'n9

co-y

of Sony

Ariosta'ir

the

"Digita

poldrizaton

ra.d'.ar

Stilcamera Dsc-s7o'

rhe.ase.

contan,

o(

Dr R.

.. .......1-10

100' Gmer" 3-20

324

,

t

a

f

€

t

f

a

6

t
t

C

e

3-2 B40{31e

t

G

e

C

f

C

e
e

f

t

e

1200

t

I

t
t

a

I

a

I
I

t

a

t

a
a

I

a

a

a

a

t

OPERATION
switch on the instrument

Switch on rhe nstrument va lh-" On/Ofl

green

The
swr.h lights up

nfumeni s

"l'

postio.)

ile

halog€n

05

The Axiostd m d6.ope s suppl€d wih
lacrory aiqned iluminalon The ilumnarion

need noi be adluftd ev€n when rhp amp 6
ex.han9ed by the.unomers themse v€s.

.

r

the requned

Set

briqhtne$ conl.o
Depending on the app icaron,

seveEl of ihe iolownq dla 32 iire6
(3-211)

um nouriield diaphGqm

nterfereice wde-band I lter,

lor coniran enhancpm€nt n b/w

photoqraphy

LED integrated ln

nd

to

lor

ready

The inteEated 6 v

lamp

munalso be on.

(3-l12).

on the duii-proiecrcn

ol sia ned

cale that ihe

operation

bighhe$

(3

22) , e
q.e€n,32x4,

secton5 and

the on/oti

Gwtch

long

20 W

pLace

one or

gla$

oi rhe

q

in

lor

I

a

a

a

a

a

+

I

a

I

I

a
a

,

CB I conveE on i ter, 32x2, to
fie co(ec1 coLor temperarur€ ot 3200 K
when aniicial lght coor .ev€6al lim s

-

CB 3, 32x2, and CB 12, 32x2, conveB on
lite6 for use wth daylisht.olor I m

qenerare

r€

When lhe LED iluminalor i5 used nst€ad of a
condensel t mun be swilched on va the
roqgie swtch, and the illhnarion ntensity

mun b€ set va the .onirc. sennq

lluminaiion s noi reqlned

homogeneous
20 mm. Ony when objective 2 sx s used, it
may be nece$ary to cents the LED liuminaror

a.d io slghty lower the condenser c.rier

lield lluminalo.

on account ol

!p to d ameter

Kdhl€r

oI

the

l3

i,

Transm rtEd'l

3.2

Transmred llqhi brlqtulield mcroscopy n the

mon usual oi ai the oplca m.oscopy
te.hniques, 5..€ il alow5 rh€ ea5y and fast
viewing ot high-.ontran or naned spe.mens

n order b obtan optmufr resolution

lumnaiion ol rhe fied, rh€ .ondenser, the

uminourli€d

diaphragm

rues ol the
Here, ihe ilumnalon.one s adaFied to rhe

oble.nve s opening .one In ths way, lhe

numer.a apenur€ oi the opti.al system s
uspd, and superiuols lqhr, whi.h.af cause
,nrerferen.€ n the fom ol scanered lqht, s

using

mun be set n a.cordan.€ with tlre

K0NLaR i

fi e tran!nitted-light

wilh flll

daphr.qn and lhe aperture

umjnarion

pin.Lp

e

qhr

qhrtield

br

3.2.2

Each A{ostar m croscope 6n be conrigured

to

When

positon

fa nlder lor
ih€ Abbe .ondenser, r mun be rcmoved
when

rranimifted]i9ht brightf ield

permit

rhe rfansmrted-iqhr b

using the

tu(et, s,at

w.g tha

s€tting of trammittedlight

Abbe .ondenser wth

ih€ brghdield

phas€

.oftast is avaiab e wirh

rransmned llqhr bnqhrlield

po5il

qhrtied

5.

on

a,

e

5
3

€

6

a

€
€
€

t

f

e

A des..ibed n chapler

r€ady for oper on

a..ord nq io s€.t on 3.1.

place

Fnn,

0.17 mm .ov€r s p on top
me.hani.aL

nage

set
contro

Move
upps stop

(3

3/3) aid move apenure

(l

l/2) to the center

a hiqh co.rrast

5taqe

brghtne$

(l

3/1)on the

abbe

ihe

co.denrer

pos

t on

75x30 R

2,

usinq lhe brighhes

micros.ope(and.

v a the .ond€fser dlve

posilon

mcros.ope ir

ihe

and swt.hed o.

spe.imen with

09/1 25 10 the

daphrasm ever

ol rhe

a
a

a

e

C

a

a

a

f

a

a

JQn'njfudJighrbnghfredro.

t

a

I
I

t

a

t

a

t

a

t

N

a
a

Transmfted-

|r5

t n.e t e d s ze
.rranqe arts
lumnoulfed
daphragm seuliqs mun be repeated
optmufr
overuew unl mun be 5wun9

swnq
Inur ed rnq

Look thrcugh rhe fred
blno.uarlube
obled va the..ar

rhen

tum'nq lhe eyelens of lhe focusing

reiuls.

lox

set the io.us for rhe oiher

a.d the obje.rv. apenure

every obJe.tve .hange,

daphragm and ;penure

io

For oblecrves <4r,

nto tlre beam

objecrve nto
(l4/t

ol rhe

(l416)rnn

a drv€11-,1/1)

beam

rcseple.e

ey€p e.e of th€
afd fo.us on rhe

palh

the

enrure

rhe

va

eye by

brqhti e d

tht

a

a

t

a

t

a

a

a

I

I

a

i

a

close rLm nousJie
it svsbe in lh€ tield

use trre
.ondenser
d

Center
usng borh .€nrerng
thEf open t unrl
daphraqm

Fof apenu.e daphragm
remove one eyep ece riofr
look nto thetubewirh yo!r
lever
to app.ox 2/3 4/5
objecrive exit
appicalons, thi settnq
d aphragm
amontu re5olution
compromise

c.ndenss drive

aphragm s ruffc enty in fo.us

4/C)

13

lun

{l',1,B)

prov

d diaphragn

yiew,

of

l/3) to ow€rrhe

1l

untl ihe uminoull e d

!mnous field

jcrews

the edq€ ot the

dsapFea6

to

iet the aperture diaphraqm

pupi (l-4lE).

de5

tor

the humf eye

iioh the n€d oi

rtrnq

ofthediameierof the

ol the apenlre

optmun.ontan dt

and fierelore the ben

(3

4/2) unrl

ev€n I not n

(3-&B)

diaphraqm

4/4) and

{3

konr6t)

fie tlbe and

naked eye. U5e

h mon

NGGOE

a

I

a

a

I

Transmired

lqhi phase

conran

.7

Uring tr:nsmittedlight pha$

3.3.1

The phase

erafr narions of rhin, u.naned

phase

The

modualoA phase
tlre iiten€ren.e pro.€dures
rordaton
the 5mal
.o or diiterences which

.onrran technique

contran ft.hnque

stop and

ot

lnlermedaie

the

phase

d ifere.ces ln intensry and

are vsib e to rhe human

spe.men5,

u5ei the opti.a

phase

hage ro.hange

sg

optimum phase

Thereiore,

ured, th€ v6be

.over sip slrface
surlac€ oJ
afd.afefuly removelrease

.onlran requires

.ean

the 3pecfre. wfi

lront led

the

co.denser surfa.€s, rhe upp€r

and $e iower.arer

of the oblectve

part'.uar.are

is rdea

rnq and

durinq the

a hiqh ev€lol

fof

e9

pat€

t
t

t

I

a
e
a

Fis 3-s tedins

ot nansntudrbht phda.ontun

3,3,2 Transmitted-lightphasecontrart

contiguration

Abbe

phase

-

When Lsng

po5ilon
poston (Phl,

Phas€
Ph 1, Ph
numerc

n

The name
ror
relevant

conde.ser 0 9/l 25 and

contrasr, e.q. Ph 2

lhe Abbe co.denser wnh 5

u(et, set the re evant

Ph2 or Phl) via

contast oblecives wth

2 or Ph 3 lor difier€nr averaqe

apertures whi.h .an aso

brighrfi€ d wilhout any.e5rrclon

phase

on ihe oblect ve, e.9. Ph 1

nop on the sider

pnase

name

oi fie

contan mln .orcspond

sider

phas€

ihe tutrer

phase

tor

nop

.inqs

be u5ed

to the

a

C

a

a

e

f
f

f
C

f

a

C

€

t

a

a

t

I

t

I

a

t

t

I

a

a

t

t

a

t

Setting

As

deKrbed

feaay

accordrng

The m.ros.ope
tramm

r

swing

40N/0

.

.rpen

aperture

Abbe

pha3€

I a

appeats

diaphragm
opened
dapnraqm

ior

tt€dJ4ht

Fhase

65 Ph 2

ruminourfield

daphragm

condansef

qop

n

rne wndow

ind.alng

.ompietery

and 'n

Transm

ot transmitted-tighr

n.hapGf2

operat@n

to se.ton 3 l

brqhtfield,

.onrrast

(:5/10),

(3

wirh

postion (or

condens€i,

thar the

(green

tho window(l6/4tare

ihe

nrro5cope

and

swt.hed .n

must

dtaphra,om

s/9)

tunei)

dot

be

as

des.r'bed in

objective, e q

inrolhe

dark|ed po,rion)is

of

beafi

(3

(.r

I s/3 on

qrce.

a

ihe .Eertlre

sroD mun

on the apedu.e

tted qht phase.onlran

s

set tor

path

5r)

and

rhe

doi

be

n lhe

a

a

a

t

I

a

a

t

a

t

a

a

t

On

the conde.*r

phase
a5 on
Ph

ruret

Check wheiher phase
.omplles
femove

d'opter

(3
out or

c€nlerinq
11

th€ rwo
urng

Then
ree<ope

conran

lh€

2, or s€t

dsk

oneeyepiece

(3.5/11)

y3)

Focls

p6hinq

requn€d,

adlunnq

a 90. ollset

repla.e rhe

Enh

(3

phase

the reryanr

(3

6/2) .r

wrh

rhe tgure

on

n

telescope

.€nrer

lre

inreft

s€) with

.ontrst

$e abbe

afd reF

or a .enternq

rhe

Dhase

th€ eyee6

via

phase

the

s.re$

heragonatkey

doprer

elep e.a

fi€

i der for

ihe

same .ane

obied,e,

posiion

.ondenser

noD

(3

7/B) For
a.€ twlh

r.q

by

{r-yi)oa

the tnuned

nop

{l516

5W 1.5

or rhe.enlernq

eq

on

th;

.enternq

rhG

the

leescope

ou tno

rh;

rna

(j

7) via

of I6/5t

!s

compiete phase.oniran

brgnr phase
dark prrase
congrueni'n

fnq

the i Lm

nop

(n

(in

is

the condenser)

rhe obr€crva)

nation beam

ony a.h€ved

path

i rhe

and rhe

are exa.ty

3-7lB)

a

I 3.7

T.ansmited lqht darki e d

3.4

Darkred
obledi rurh ai ba.rera, but a5o ior emuslofs
or unnan€d oble.G in waterysouions

In

Van5mfted
boogca spe.mens, such as ba.refla or ivnq
cei.uturcs, are oltef barcly dentlbbe
al on a..ounr of rh€r lqhr trafsm$on The
sluaton.han9e5sgnil.anily f
afe viewed n iransmitedllghl darkled In

prin.iple,

luminanon

only the diltra.ted and
.ompon€nis !vh!.h are so impona.l lor maq€

produarion

dnedy relearinq

Uting rhe t.ansmnledlisht

s many used for sml or mnute

lqht

the

spe(men

apeliure

rea.h

brqhdield, unnaned

such!pecmans

is ilminaled wth an

whch

the

ghl

r a19er rhan ihat

(attered

oblectve, wh e lh€

bundle5

g!ded past

are

iiar

lqht

rhis i3

ntuduercan be resolved and appear &
a dark background athouqh they
b€low lh€ r€iovinq

3,4,2 Iransmntedlisht darklield

one of the

condenrer
Wh€n usnq the Abbe condens€f with 5

pos

t on tutrer, ser the darki e d oosrion

(DF,0

CS oblectives

whlch s smaler than

For lurther dera s,

wirh s d€r for da*fi€ld

55/0 9)via rhe tudet d sk.

re.sons why

power

wth a nums.al aperture

that o1 the used

pease

see the followi.g

even

panaly

ol the lqhr

fne

qht

on

ie

t

t

t

f

f.

f

s dg l.rdJ,Ied o,5Ds

fordrrrPd

i de'

o 65 0elof40)

LDt

f

e

|0)

C

o

a

f

c

f
f

a

f

C

e

3-3

e
a
a

t

a
a

a

a

a

I

a

a
a
a

I

I

I

Tranrnred

3.4.3 setting

As

descrbed in chapter 2, the mi.roscope s

ready
accodrnq io secr on I L

!

Ser

ror

.

n5ertrhe reevant dde.(l-&3)lor darkfi€ld

­on the .ondenser unt nop

'

Open thelumnourlield

.

ncrease

fof opefalon afd sw ched on

KdHLER i

nanrmtted

se€ tabLe on

the

ol tranemitted light

umnatlon ln the sameway as

qhr

paqe

brghtne$

qhrli€

br

1.3 nlo the opennq

daph.agm

d, eq wLth

senmq on the

qht

da*ie d

6

Darkle d spe. men5 requne a .onsid€raby
hgher eve oi .eanine$ lhan spe.imens lor
olher methodst tnqerprnls, drt and dun in
pan.ular

the conlran

brighlen the ba.k9round and redu.e

ol lhe oblect ima9€

-.'-2

t.

t

I

a

I

t

a

a

a
a

I

a

a

t

.

For darkfed 5enn9, I s aso
lhe sider lor
1o

owng oblectlves
darkfield
Pan

inagecenre.

flhe

*nstlvely co(e.r the .ondenser heiqht

unll the ie d ol v ew appea6
hohogeneousy dark or J€ailres
homogefeous b.

0 2510 9 CP Achrohat, A

Neoiuar 2.5x l0x and A.hrop an 4x

stoo brishr oftoo da.k,

qhh€$

phase

instead

disiribul on

po$ible

conrran 3 and rhe

oi $e s der for

Pan

to use

and

a

a
a

a

a

39

I

Transmifted- ight

Using

3.5.1

The iransn lted-lght

used wth specimens
polaizaton

nate oi ight Such spe. mens, e.q.

cryslals, mineras or

vansmittedlight

polazalon

which

poyme6,

technique s

change ihe

ar€ lermed as

bnefiing€nr I bneiringenr sLbstan.es are

vewed

betvveen cro$ed

poLaizer

analyzer), they appear

brght whie

(polarizer

L

the

surou.d nq rema ns dalk

polarzation.oniran

Brefrngent slbna.cer are

lact

postions
otatio. of the

Dependinq on the

thi.kne* a.d oi€nraton

nt€rler€nce co06 wilo..ur
n b o oqi.a obledi) to

blue.

they dspay 4 brght and 4 dalk

that

betw€en

These nle.ference colo6 can

r

Po arzer, orient€d

mounied on swing

recog.zed

.rc*ed

spe.imen ablnd 360'.

leve oI bnefrngen.e,

trom

white,

Traff mittedrisht

in EAST wEsT d

out cadier{3 9/t)

by the

pola

ze6 aft-"r

ot the obiect,

(usualy

Itay

ye

low, red and

be ol 1'

polarization

or

recdon,

Flg'39sedinga|b|'oiftd]iqhtpal.iBnon

s€trin9 of

As des.ribed n chapt€.2,

ready for operalion
accordinq lo iecton

The
ransmitl€dr

m..os.ope must

ghi

5cr€w NORTN-SOUTFIoaemed
(3

9/2) inro the tlb€
nand) from below.
orientaron is ava labLe
on the
longiludina

Swing
rhe

analzer

axs oI the sta.d

polarz€r

field ol vi€w appeaE dark be.ause

tranemittednisht

the mcroscope is

and 3wthed on

3.1
b€ s€t ro.

brghtfed, a5 des.ibed

analyzef

(removed

lrom the

NoRTN'solTll

wh-"n rhe ma*ing

(3

is

algned

nio ihe beam

9/1)

along the

path;

in

ol

Move

fed o1 vi€w. Bnefringe.t
oblecB

rh€ objecr ro be examn€d

(anisoiropic)

now lsualy exhibn rhe

inlo lhe

optca

I

a

t

a

t

I

a
a

t

a

t

a

a

I

a

a

3.5.4 sample
.

Move

(anaryzer

EAST

.

swnq n lambda

ambda

0000'000),

.

5e eat.ry5la needles which
the

t

t the crystalneede5

qamma

y€low

right anqe
blue, lh€

two

NoRTH

WE5T orienred)

pate

qamma

dnecUon

and

difi erentiation between

porarizes

soUlH-o.ienred,

pate

ir

availabe

set lhe osci alion d rection

dre.ion

oi the ambda
the aysta needles lyng at a
to the

crysta s are monosodum urate

Transmrtedl

ro th€

Gee

o.ienled

gafrha

dar(

and, r{ a rotary

(e.q

afe orented in

marklnq on the

paEle

dn€aro. are

ght po

posiron

po

arizer

445226-

ta

toihe

piate

are

arlzaton.ontran

a

a

t

I

a

a

a
a
a

t

I

a

.

lf lhe

cfystalneed es orenred

gamma
b!e, and the.rFta needes

angle to tlie
Uie crystals ar€ .alcium

Ths

analJ6 s is aso

wth .emented lambda

placed

.ase, the ambda

on rh€ lumi.ouiie

re.rion

d

gamma

plate

of the ambda

po$rble

needs

parallel10

pate

ynq

al a riqht

dneclon

pyrophosphate

using

plate

which .an b€

d d aphraqm. n

notberotated.

are

poa

a

yeLlow,

lhe

are

zer

that

a

a

a

a

Jrl

Measu.emenrof lengths

Tlre m,aaiurefrenr
requiies

$e lo lowing, forexample

naqe microfreFr, pos

eyepre.e .ro<lne mdometer

An

oveto ew ot avaiabl,a

and eyepie.e reiicl€s is

ol lengths

tive 5 +

|r9

Ihe

dstan.e to be m€allred

n fie rnterm€diate

keep lhe influence
dev arions as ow a5
other measurng
eyepr€.e has not

eyepiece mage n

ol random measuang

po$ible

ero6 .an o.cur

been nserted into

uing tho Aronar

100/100 y D

10 :

stage m.romete6

provded

should be> 5 mm

100,

n .haptef

order to

f the

the tube

Belore the
m(os.ope

or s.a e value

relcle
scale
spe.imen wh.h.omp
lsed eyepie.e..o$ ine

For
m'crometer
paralel1o

and make lh€ zero Ines of

f, ior example,

ol |re

100 ncrenenrs

n Fig 3 10,

used objectjve / €yep e.€ relcle

(A

I

combnation mun be

yalue

caibration, aiqn the s.aes

stage m oohet€r .otre5pond

Plan 10xl0.25 and

-

:

englh measuremenr

can be

and ihe .ro$ine

ea.h oiher by iurnng

the resutnq s.al€ value

pertormed,

ol rhe used

s exacrly

99 n(emefts

of rh€.ro$ln€ micrometer,

0um=99!m

lhat dista..e n

es ro on€ nt€toa

micometer

c.o$Llne mi.rometer

lhe fricromeref

oblecrve / eyep

determrn€d Ths

both s.ales exaddy

(of

urng the

ece
the

of lh-A

of fie staqe

m crom€ter

the eyeprece,

10

combnaton

each)

!m

to exa.ry

as

k' ior the

,

I

t

f

t

a,

t

--=o=----

Fl9.310Lengthna'urenent

Atterexchanqe ol the nage m(omeleriorlhe

speclnen ro be measured,
dnance ol interest L r€sulls

ol n..€nenll

m..ometer

L=155t99!m=351
Paruculafy

delermrned by 6nq rhe vern
qradatons (0.1

l-lere, ri mqhr be nece$ary
d nan.€ lo be measlred

lrom

a comb.ed x and y meas!.ement

oi the eyep e.€ .roslne

{tenih

arse obj€.r ntu.iures

enimared) muriplied with

5pm

mm) on

the

lhe measurng

irom ihe nlmber

can also be

er scale

mecha.ca

10 determ n€ the

tholqh.a.uation

naqe.

l-12

a

t
t

t

t

a

t

a

a

t

a

a
a

a

a

Ana.hm€nt

.

Loosef
and

t

Remove
unde6de and lrom

'

Hord

slqhty n. n€d
req!

knured s.r€w

lo .amp t nto
1l

the
A.cording y,
iubeare

.

Renove
aid nsert two ey€pieces

knured s.r€w

renove b no.uar

du5t

c! doubletube

red vl€w n9

1t5) Bolh

90' bac(wad rotaloi

acce$bl€lo the

dln caps lrod

cov€6 from

postion,

l/4 or heragona

13-l

rhe i unraled rotaton

Lhe .onror

Atiachmeft

of

Cs do!bte tube

(3

11/4)onthenand

tube, I ava

rhe

the

doveta I m.unr

(3

I ln)lolhe t€ft

nsert

tlrn

d re.ton and

the threaded

or the

!serlrom

the €yepiece

€a.h

ts dov€tai

1 nlo ihe

are

c5 doubte

(3

rr/2)wrh

ab e

Ube

ir a

uje the

soew)

open'ig

and

po$'be.

rhe s de

rubes

ot .5 doub e

on

@

tube

a

a

a

a

I

I

t

,.

a

a

a

a

a

t

ns€rt.ounrry speclJic

power

rhe

t

nsert anqed

powerunrrntothe

'

Lrserhe enclose.i

(3rrnr)ro
nand and

t

Connecrpower u.tloth€

The

Cs doubletub€ soperat€d

r

Adjlnns ev€r
oithe

r

contro

unrr(3-rrA).

connector

5eladherve.abo

su taby atlach

quide

ghr poinrerin

{3.1116)lo

tio

(:-llt)lof

ser rhe

adapter

1l

t2vs.(kei(l-11l3)of

the.ab

the ba.k

ine

$encos.ope

brghtn€s

1tl9) i.io

G

I t/10) .t

e 10 the

vial

po5tronn!

the

the

holdeE

imaqe

oJ lhe

']

4 Knu

-{,

ed rrew

h*aqon:

lor

arew)

a

t

a

a

ll

Pholom

noqraphy

equ

pment

3,a

photomic.ogr.phy

€q!ipmeht

TheA{ostar
.hanqed
nicroqrapht
atach€d

lpnobmcoqraphy
!.11 nop,
ol tlre rnob,

eyepeces
.ommer.ialy
sp€cla
beana.hed

It

fo.us n!
ot lhe
eyepece

eyepreces

For

TheiJoos
andihe

mraoscope

lom

via the

to borh

ie r00

the beam

(100

% vt

avar able

mi(os.ope

to the

is nor

.am€ra

ren.e

(ahos€esecuons1

focurnq,

ro be made

mun

set lhe

untl both

relcre.r€

wfi

observaion

knob

€

sdes

Fostro.:

o/o

caneras

cam€r.

rhe

or the

turned

doc)

. fo.us

n the rear posilon

Fa$

spe.a

35 mm

{e

port

va rhe

compone.t

be screwed

5 and

eyept€.e u.tit

the m

photoiube.an

ro

lzt

a.d 3 t2l3)

photorlbe

to the tefi

s

dne.ted

adapt€r

SLR.am€ras

Mc BO

9.
of ihe Ax

v€Minder

wth

2.! 1)

(onope

oholo

to lhe

atow

and

Dx) io

osta.

ihe

h

the

rhe

maoe

be

rig'312A&achnejrorsLR.ane?.

r,3,t

.

\.rew

bayon€t

{3

.

Arta.h.amera
rerease

Attachmenl

T-2

adapter

on the

l2l4)(4s6005

oooo.ooo).

housn!

(l-12n),

if requned.

hexaqonat

remove
phorotube
pr€mounted

r

A'gn
pornon

screws

.

For

rhe dun

tlre

Gmsa

and iglitef

I2l5).

{l

photom

.roqraphy,

(:

t2ls)

(3-r2lA)in

unr

!9
For

the

use ol

equrpdenl, pr€ase
separar€

manuak

in

additon

of SLR.amera,

(J

I Zj) lor

2.5x
(3

rh€ CONTAX

connector tor

t2l2)a.d

screws

.over

(3

t2a) from

and

the

!nt in

the

the required

thre€ hexaqonal

rurn.ontro

pholomicrography

the
obse.ve

rhe reev.ir

rorh€ inlormaton

T2

cabre

(3.r216)

1he

nsert the

pholotube

(3

lzz)

I

f

I

a

t

t
t

a

t

a

t
t
t

a

t

a

When artil
temp€rature

(3

z2), as mentoned

For

dayighr coor r€veua lilm,

various T2 ad.pG6

12 adaprer ror

n 3d3ph' fol d|NofA i-3 b:Fid)

12 adapF ror

l9h1

ca

ol 3200 K. The I ter mlst

color reveEai ilm

n.haprer 3 r

for sLR(amerasare listed below:

oTYMPUS OM

CANON

Pholom

$e CB 12 .onv€E on liter must be us€d in addtion

(oM

bayondr

bapner)

1ro

aogiaphy equlpment

5 used, the CB 3 conveb on
be p aced on lhe dun cover of ihe

llter

provdes

luhi.ourf€

$e cotre.t

d diaphragm

to the CB 3

cotor

a
a

a
a

I
I

a
a

a
a

a

a

a

rz:daprerrdrPENra(rA

For detaled inlormarion

Dhororr.oadphv..r9l5rn.,q

bapien

on sLR camefas,

pease

!a-d tor. d t/eh\

s€e the new operaunq

a
a
a
a

a

l-15

Pholom

(ogEphy

€qu

pmenl

3.8.2

.

Loosen heiaqonal 5.rew5

remove dun

nsen
(3
(l13/5)

nse P

adapts
(3

Ata.h MC 30or bas. body
adapter 60 f.f m.ror.ope
nop,

r n! € r38)

Ataclr l9 mm Mot DX t m .aseiie
(3

that the .onta.t p ns irm y enqaqe n the

Attachm€nt ot Mc 30 Dx

(3-l3/7)

prore.rion

adapler 60 tor mtrroscop€

l3/5)(456006 0000 000) inlo

and tjx t Ls.q thr€e hexagona

prqecr

2 5i

60 ior friooscope .am€ra5

I3/5)

ailn t and l t

a.ric ockwie

13/1) 1o the basi. body in su.h a way

cap

{l

on ens

by iurning

(3

{3-l:2)

camerar unii

l3/9) lrom

phototube

13/4) nro

clafrpng

and

.aoera

onto

ror microphotography,

when artfl. a
the CB I .onveBion ilter
.olor temperatlre of 3200 K The filer must

pla.ed

diaFhragm

31
Fo.

.onve6io. f rer h6r aso be u5ed.
mlst

luninous fe d daphraqm

For dera ed info.matron on lhe MC30Dx,
p

on lhe duit

daylight color reveGal

be

ease s€e manualB ao-036-e

qht

.olor rev€6a I m s used,

covef

(3

2/2), as mentioned n .hapter

Fa.ed

on the dun .over oi the

t!r. co.trol(3 13/3)

provd€s

ol

rhe uminous

flm,

(l

2/2), as des.rib€d

th€ .orect

be

leid

Ihe CB l2

rhe

urr

a

,

t

operaron

Attachment of adapteB ior video cameras

a

a

t

t

t
t

I

t

I

t

I
I

I

3,9 Ana.hment of adaprers lor

the lo owlng

adapte6 with

vldeo adapte6

nterrace

and

60

video

permit

zoom

the

l-chip b/w and color

cameras to the

phototube

.am€rar and l.hip ccD

ihe ariostar.

ol

I

a

a

f

a

I

a

I

a

a

I

a
a
a

I

,l

Attachment

-

60

44 also

interlace

Operation

oi

adapteB for vids

allos vids

to be us€d with

phororube

the

Gmeras

oi rhe

Ariona. with

60

mm

!9

The

instructions

must

attacimont
.

.

.

t

al$

be obsetoed

a.d settingi:

Lo@n

dust

thrce hexagonal

@er from

sc.ew video adapter

wn c,molnt

Inser(

video adapts

in

ENG 23'

vdeo came..
hsen

video
camera tube
it using rh€

prenounted

adapt€r

oi the Arostaf,

thrce hebqonat{rs.

Mi.l6co96

oi

the camera manufacru.er

whh iilere

when opeErng

sc.es

rhe cam€€

o. video

thread

or video

or ENG r4,,

and.amp

or vids

it tiqht,

(video

unh

zoom adapt€r)

and r€move

tube

of the

u oon

inlo

adapter

the vdeo

200m adapier

bayonet

of the

camera

atiqn

it and fix

the

with

in

@

mm and

l.sert

eyepiece

binocuar

Swing oLt
phoiorube

me camera
5et the requned

via

the wheel

I requn€d,
mo.rtof

wlth

rlbe

and allqn

foldifg

to dnecl

pon.

zoom magnifiGtion

of the video

adjust image

by

changi.g

phoro

prism

rctide
photo

in the

rettcte

on the bin@tar

100 % oi the tighr

factor

zoom adapt-af.

brightness

the limp b

on the

ghtngs

ro

3-13

B 40-031

e T2l00

,

c

)

OPeration

Attachment oi adap1e6 for dlgiralcompact

cameEs

c

9

I

I

a

t

a

a
a

I
I

a

3.10 Atrichnent
digital

@mpa.t emera3

Ei#,*,,,

of adapteB lor

t

a

a
a

I

a

a

I

a
a

a

I

I

a

I

a

a

840,031e

I2100

3.r9

Attachmenl

ot adaDle6 ior

Operaon

dEta

compacr.ameras

.-l

3.10.1

When d€lyered,

(3-14/2)

Ml7

premolnred.

Refrov€ rhreaded

13

Pluq

bino.uar

S.rew

wth
{3-r4l4),

wrth
totuard,

(3

Digital
fillerthr€ad,
PCr00

the slidrng

and the len5

The drawing

15/3)

connecror 60

phototube (l-raA

the !ni consistng

tfrread Ml7

nlo rhe M3T riher

the lens mounl

plug

l4l5) lnt

cahe.as with

e.g.

/ soNY Dsc-

mouft wrth thread

mount

on the rghr

adaprer rng

(lJ4l6)

{l-14/2)

(3

the lnt onto conned(or

stop and tghten the

37 mm

SONY DCR-

550

(l-14l4)are

rhos

M37lM52

onro the

and ighten

idinq

of I

and ens mount

rhread ol

1414)

mount

rhe

pointing

60

set

q''.14A64nentoffuny"Digita|

Dependi.q
and the
the .amera

perhaps

mun

This s required

mage

postion
Make

the forlowinq

t

Swnch olflheaubfocus

.

5el

r

let

.

sei the apeture
se ecr a smal

Not

al caheras ieature

Please se€ lhe

.

Loosen

Varylhe dista..e

and rhe

sidi.g mouft

on the mi..oscope

canera used, the

ens and rhe

be opiimzed

in

cannot be

ol the

the distan.e

lhe aulomatc

Gmera ens

i nop nlmber

operaiinq nnrLcrions

the s€t screw

ens mounr n n€p5, ie move

with .amefa

co iguraton

dstance betu€en

ens frount

doub e atrow).

Gee

paadcular

achieved n any zoom

sett nqs on the.am€ra:

toa.

erposure co ro to lime

as wd€ as

betueen the.ame.a

ii an untrimmed

).

these

(3.14/3).

(l

144)

po$ible

po$blit6.

on the le.r

(

ol rhe

e

ens
the

,

I

Alla.hmenr

ol adapte6

Operalion

lor

d 9 tal

(ompa.t

cameras

t

I

t

s

I

a

I
I
I
I'

t

a

a

a

a
a

r

zoom cahera l€n5 lrom wde-ange
po

.

Pertorm len unt image
without l.mm ng

t f ghten
No untrmmed imaqe may

.amera adapt€r ..mbination
nol r€.ommcnd€d by

3.10.2 Dtqaial

whe.

the fireaded adapt€r rinq M37lMs2
and ihe ens mounl

The dEwing on lhe right shows the

Furthermore, the
Ml7/52
srldinq mount
.amctas wth M!7
deso bed n the

(W)

on

deivered, rhe s dng mouni

(3

lhrough ro

viqnettinq

or

the serscrew(l 14/3)again

Ca Zei

(am€ras

filterthread,

e,9, SONY DSC S70

(l

lV6)are

threaded rnq adapt€r

l5/3) .an

be unr.rcwed frcm the

(3-rta) (noi

can aeo be atta.hed as

previous

chapt€r

pos

&e

be obia ned when a

with 52 mh

lon

is iormatli inq

B used which is

premounied

shown) €

(r).

(3.r9la),

€-r5/3)

(

t,

t

I

I

a

I

a
a

t

a

a
a

I

a

a

.

screw SoNY VAD s70 adaprer ring
(3

lsl2) into the oble.tve/ ter rhread ol

tlre D5C 570.amera

.

PrLq .onne(or
bnocuar
lhe 3 sel s.rews

.

s.rewrh€ unt,
(3.l5/4),

Ml7/M52
(3-15/6)

| ^9

.

With the €n5 mou.t
foMafd,

(l-r5/8)

phoiorube (r

the

(3.15/3)

inro

lhe SONY VAD S70 adaprer

13-15/2).

pluq

the lnn onto .onnecror 60

untl nop and I

(l-r5/t)unti

(3

60

(l

1Vlo)

cofsistlng of sldlng mounr

threaded adapier

1s/3) onto the

r5l9) and rghren

and the lens mount

(3

ts/6)

qhten

stop.

rlng

pointnq

the 5er

321

Ana.hment

ol adapteB for digita

oper on

compac(cameras

Depending
.amera
.amera rens

perhaps

s reqlir€d in

cannol be achieved

Make the io owng Ftiings
r

swii.hofltheautoiocus

.

sel lhe allomalc

.

set the aperture as wde
seLeat a sma I'nop numberr).

Noi a .ameras fea
Pease

t

Loosen fie sel s.rew

.

Varylhe dtlance

and the len5 mount

siding molnt

moLnt

r

zoom camera l€n5 irom

porn

'

Perlorm

wthout

r

Tighien rhe s€t srcw

No

untimmed maqe may be
camera adapier.ombi.aton is
not recomfrended

on $e mi.ros.ope

used, fie dstance

and rhe r€.5 mounr

be oprim zed

paftic!

5ee the opeGtnq innrud:on5

by der ned steps.

on

M)lhrouqh

len until inage 6 lormat-ili.g

lrinnlng or viqnett nq

Gee

ar if an untrmm€d image

n any zoom

expoiure.onrro roiime

rc ih6€

belween fie.amera lenr

wth camera on the lenr

by car ze$

conlguratlon or

double arow). Ihc

o. the cahera:

as

(l-195)

n steps, .e. mov€ the

io ie

e

(3-15l5)aga

obtaned when a

between ihe

(3

I s/5) must

posrion

po$ib

posiron

used whch s

e

{i.e

posibiltEs

ol lhe

wde-a.g e

(1.

n

of

Atta.hment of

(am€ras

with

other f ilter thread sizes

prncpl€,

n

r s aso

cameras with alilrerihr€ad orherihaf

M52. Suitable tilter adapte6
are avalabe n
.bove,

only a test can clailfy whether such

photo

po$b€

red!ction

o.

5hops.

As

ro

connect

M37 or

rngs

mentoned

camefas are comparbe wth fie dqia

3,10.4

00000G'1103-934

Ihis

adapr€r

rhe

so.y DSC-S7o Combined

has

objecl vB, e.9.

.creaed

edqe definiton and ower d nonion

can be achieved shoud futlre cameras

pan

been

cu arLy designed for

Plan-Neotua

wnh

Plan

objeclves,

pov€

to be surable, roo, our saes naii wil be

Aiia.hm€nr ol the .amera requnes the SONY
VAD

Furthermore,

(456140-0000
the

canera adapier and ihe binocllartube.

Make

.

swt.h ofi

.

set the automal. exposur€.ontro rorime

5

70

adapter

the

000)must

.on.ectn9

rng

be

$e folowinqrtli.gs on th-acamera:

aurolo.us.

the

(Fig
pec€

xxxz).

60-44

nserted betreen

122

.

Set the aperture as wde as
select a smal f nop number

possible (i.e

).

t

t

I

t
t

a
a

t
t
t

I

I

a
a

I

I

3_11

portable.ase

The

to transpon the basi. Axiostar €qlipment
Gtand

mechanical
r

.

.

t

wilh

stage,

Belore irsertinq the Alona. inio ihe case,

loosen the knured screw on rhe stand
(3164)

b nocular rube
(eyp

eces

the knured
Open rhefolrlockr olthecarand

case cover
rrom

rhe case iloor

nsen m.roscope nlolhe re.€$ in the.ase

Push rubbef roam
the conde.ser
conden5er upward5 until

rubber foam

lor the Ar onaf

bnocuar tube,

ne cable,

by rlhnq :t by Yr. turn rh€

(3r616)

poiniinq

screw.gain

(3

component

ba.kwardt and tqhlen

1611) upwardr io remove
{3

component

(3-i615)

Abbe

dust cover).

around r30'

162).

and

it

be

can

(3-16/4)

contacls the

lsed

condefser,

plLlthe

ovef

move

the

ir

Pu I the bino.u ar tube

apanand s id€ rhe rubber ioam compo.enr
(3-r6t)

slop t

pre$ute
unrlihe

wththe upper sland

Lne cabe, dun cover and a.y smal

a.ce$or €s Gn be p aced on the case il@r

bes d€ the

rhe V€ cro faste.eB
Attach

snap and,

b€rween rh€ eyep ece lubes untl

mighi be nece$ary ro apply some

on the

holding iaps

(and

and iixed in

the case

cover, .lose ihc ockswtha

il requted, lock rhem wilh the

(l-1616)

rubber foam .omponent

(3-r613)

part

(3

1 6/3).

compl€rely

arc llLsh

pos

tion using

a

I

a

I

I

t
t

a

a

a

a

a
a

I

a
a

B 40 011 e r2l00

323

a

f

€

a

€

€

6
5

a

t

I

a

124

€
€
€

C

€

C
C

a

G

C

G

t

Care, Mainlenan.e, Trcubleshootng

t

cont€ni5, il ustratrons

t

t

t

t
t
t

t

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I

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I

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t
I

a

I
I
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I

CARE, MAINTENANCE AND

TROUBLESHOOTING

I'oub1erhoolinq...........,..,,,,.,..

Chanq nq the t!5es....

4.2.2

43

chanq nq lhe 6 v, 20 w ha oqen lamp . .. ..

Troub|eshoor ng lable.. .. . .. .. . .. .. .

'abe

or spare..

Requenn9 serui.e

Changng thefuses. .... .. ..

Changngrhe 6

sea"

v,20

pa,rs

9

W ha ogen

at roo.

lamp

. ............

A2
4-3

4-3

.

+3

a
a
a
a
a

a

t

a

a

I

Care, Maintena.ce, Troub eshootng

Care and marntenance

ol the

nnrument

4 CARE,

TROUBLE5HOOTING

4,1 Care and

Care of rhe Aionar s imred

oper.tons

.

Cover the nstrument \!th
aftereveryuse

room,

.

Covs open rubes wilh

caps.

.

Remove
arla@s 6ng a brush, anb
optcs cean ng

.

Remove watersotub e
er.) alter breathlng on
wrh a dunjr*
moii€.ed.loth
also be add€d to the warer

.

Remove

(immeAion
ol conon or a du(nee

dipped n the opt.s.l€an.9
This .eanng mirture s prcdu.ed
90 Volo/o

sopropanol

are aso known under

e max hufridny<35olo

dun and din trom vsibte

MAINTENANCE AND

naintenan.e ofthe

to rhe lolowing

rhe dun.over

dun

ower,

paper

or a

conon c oih

(coifee,

dtrr

t and wipinq I olj

cotton .oth or

A mid cean no aoeni.a.

srubborn, oly or

ols, iifqer

benzolne and 10 Vo

(rPA).

prnls)wth

rhe

various .omponents

greasy

cotton .loth

m xrure L

the fo owinq

prolecton

optca

Qip,

coke,

di]lt

a wad

oi

%

When
zones,

,

The rsk ot

fie.hanical innrufient aways €xist n

lolow ng.ondit ons:

-

!5ng the

p.oceed

store rhe Axiostar n biqhi,

v€nliat€d tooms wih a humidily

.5d

obledives and eyepieces, nadryctoser.

RetaUve humdry

remperalures beiw€e.
lor more

Dust deposts and lngelprnts

5!aces.

3c

nrta aiion in dark rooms wthout ar

Ariostar in

lolows:

as

qo

.ro..

growrh

than three days

o.n

ol lunqus on

more

of

+r5.

hlmd .lmatc

dry and well

of le$

.rtarr se-nrr"

than 75% and

C and +3s" c

on opticat

opio-

the

benzoline: medcalaL.ohol,

pevoeuh

lsopropanol

C€an the optca surface by movrng in
stannq in the mddle sliqhi
ex€rtedon the optic dunng

42

prop.no,

2
dmethyl.arbnol,
2 hydroxypropa.e

ether

pr€$ure

cleannq

sholld be

c r<les

care, Ma nt€nan.e, Troubleshoolinq

Trouble5hootinq

4,2

Troubeshooling for the Axiostar s d€s.rlbed in
lunh€r delai us nq the iollowinq lwo examp es:

changing the

'

.hanqinqih€ 6V,20Whaoqen lamp

Fu her

measures are summaized in thp tabe

luses

a.d

€9

.

swlrch ofi the initumeni
swt.h
rh€ ine and disconnect

,

.

Check

(4

rn), dhconned

line.able and

t

.

Use a 2 0 mm cockmake/s screwdivs

t

I

pre$
sd€s oi fie tuse hoLder ovs rhe 5ol and
remove lhe ilse

in the 1wo sprng shackles on

hoLder

via

rhe odoll

plug

ne

llneconnectof fiom

puss

and exchange

(4-r/3)

fiom

lo

the

I
I
!

)

I

I

a

t

I

a
a
a

.

Remov€ dele.tv€ tuse nsens

lus€ ho d€r and repia.e them wth new

rhe

.

Reinsertfie

luses, the spring shackles on the sides mun

bly lock inlo

aud

T0.34;250v;9

For the
please

.ataloque numbets oj luse lnserts

se€

fuse

p.q€4

holder contanng the

Place.

r 20 mm fus€ iruens mun be

3 ir searion4.2 4.

(4

1/2)

Iiom

new

I

I

care, Maintenance, Troublesh@t

Trcubleshootnq

ng

changing the 6 v,20w halogen

i

!

Do not

handsj il
alcoho betore iwnching il on
to

The lolowing

touch the amp bub

required,

prewnl

dinfrom blm.g

pro.edlre

cea. lhe bllb

ror

n.

is requ red 1o

6D /-\.

switch oll amp slpply

and allow lamp to coo dow. tor app.ox. 15

osconned the eanh-contacr
line and remove the

Place rh€ d sco.necred instrument on 15
back 1o mak€ the undeBide of the

Fold

down cover

deiective haloge. lamp

uie the
6V,20W halogen ahp and insert both
lamp

prot€cive

pns

ca relu

Ly

lnro

rs

The

2

Ar ostar microscope
iaciory-aigned llumination,
need not be adluned even whenth€
exchanqed by rhe c!51ome6 thenrelves.

via

lar

(4-2)

(4'zi).

cover to hold the

the ecepracles.

is slppied with

your

with

with

th€ ii6t time

odoil sw'lch

pllq

pluq

afd

Ihe lludination

bare

clean

iron the

irom the

pyramid

pull

olt

new

lamp is

-,{

.I

r--

n

,{

f,t

.:{

,.{

{

',{
,,,

{

,'i
'',

i

,:,

I

hs..2 <hanslns the 6 v 20 w h't6sen t

np

Fold

up covq agan, reruh
uprighi

poshion

and .econnecr

the sland to the

fie nand to

840 031 e l2l00

na

,r

a

..,I
,ta
',I
,t

I

,I

,ia

t

Ma ntenance, Trolb eshooting

Care,

t

lroubeshoorn9

I

t
t

.|

t

I

t

t

I
I

'|

't
t

.t

'f

4.2.3

Move lh€ v ndo. knob on the
photorube
porlion

Nosepiece

Condenser nor ser cor ecr

aperture d aphraqm

Luminols l e d d aphragn

Filiernot nsefted conect

is noi n ihe rorecl

(intermed

wnh

obleclive

ale

nor

posi

nol switch nosepiece

sei

y

phorotube

on)

y

not

in I Lter ns€rtiiier co(edy

(end

t€t apertlre d aphragm cotrecty
(.enternq

5er lumiious

set

.otre.rly

10 rhe cotrecr

poslionl,

ree p I 14 I 16

open

,

fe

(cente

wih

nq),

d d aphragm

n9, apenlnq),

posil

objective

see p :-5

n r lrer

on

see

I

.l

't

,|

t

I
I
I
I

a

I

I

a

Aperture d aphraqm opening not

Conde.rer.ot

r ,;iiiq i""ii iip thilr""i'

!:ll'r.:lrll:1.19.!!

imme6ion olwlh CZ imheaion

lo.used.ore.iy

iii

?!regrv!:

-

9!!!91": .................

Af bubb e5 in rhe mme6 0n or

mmpr.n oi alrhe{ront ens ol a

!1 9!r:!1|: ..............

co( rng s

nor set ro r1r€ .ofecr

aperture d aphraqm openin9

Sei
in

accordan.e

or depend

wilh lhe 2/3 rue

nq on lh€ spe. men

19!!"1!::*!: l l .............

!g!!T"tr :9: Sr.l.',4r.:.:..

f:!!:

Ure $e core.t 0

1 7 mm .ov€r

:l!:r:::.9: l.!.. .

cZimm€Blo. ol5rB

U5e

Remov€ an bubb es byapplyinq
.ew ol

c ean the fiontle.s olthedrv

9!19!ltv:r

setlhecotr

o. by mov nq objective

':9

!:.1.1.

rinqiofiecotrecl

N, se€

a

I

a

45

Care, Ma ntenance, rrolb

Iroubeshootnq

erhoot

n!

ioc!s

Greal

Lell .nd .iqhi I e ds of v ew

.annot be.omb ned rnto an

d i'erences ailerFo.ueng eyepi€.es are nor sel ser fo.ui ng eyep ecs to th€

Drtor dun on

Condens€r not 5el.ore.ry

Nosep ece not.offeciy

Sp€cimen

nrerpup'

lr'nocu

Focus ng

nrerpupilary

binocular lube s not ser.ore.rly

a. tube is not se1.ore.1y

the opticalsurfa(es

swl.hed Corearly

.ot

tr

iixed on rhe

ary dislance ol tlre

eyep eces a.e nol set set

disian.e ol the

.L .k nop nos€pLe.e

spec men

app.oprale ametop a, see

holder and lril

p.

24,25

nrerpup lary d nan.e

Set

lo.ulfq ey€pLeces to the
appropnate
2 4,2-5

Set core.t

ametrop a, s€e

inlerpupi ary d slan.e,

p

aLlhou9h

rhe on/ojf sw r.h

Fo.us ng

maqe

Condenser noL f..used.oredy

Aperlure diaphralm open ng loo ser aperture d'aphragm open

Di.l ordun on fie

condens€E,1lt€6 or spe. mens

Lne cab e nol

etepeces are iot set set

b,iqhh€$ nota(eprabe Reduce

opi(a suda.es

conne(ed lo rhp

1o.!5inq eyepieces to the
appropnat€

n a..ordance wth the 2/3

or d€pend fq on rne spec men

ametrop a, s€e

lamp voLta9e or n5ert

p

rue

cl€an rh€ opt.a suria(esoi the

conne.r I n€ .able 1o the

nea.d

'nnrumem and rn€ vonage, see
p.212

ng

t

Care, Mainle.ance,

t

Troubleshoorin9

Troubleshoot

ng

t
t
t

a

t

t

a

,

t
t

t
t
t
I

I

6 V,20 W halog€n lamp not nnal 6 v,20 w haloqen lamp,

q

11

w halogen

V,20 W

6

halog€n lamp def€arre

:i9

Fxchange 6 V, 20

191P,199 P: t1

The

rpecfed 6 V,20 W ha oqen

E

ecrron cs

End of average

Incotrecry instaled

pins

The

amp are

module

ol rhe 6 v, 20

not

po$by

lle

of 6

or brok€n

corccly

v,20 W R€pLa.e 6 V,20 W ha ogen lamp

w halogen

ifserted 6 V,20 haooen amp into

Use th€

!9J,o: L-9.............................

llJ.,o..,a

llave e edronics module checked
by m .rorcopy seMce ano
r-Pp aced, i rcqured, see

Ine cor€.ty coinect

sp€.il ed 5 V,20 W

l

p.4-9

ine

cab e or

a

a

I
I
I
I
I
I

a

I
I
I
I

I

a

adr€ Ma ntenan.e, Troubl€shooinq

Troubeihootng

I
I

a

Table ofspares, wearihg pads

a ? rir

tWSba headeds.rewdrve,
9w2 90' ottsel hexagona key

Dun.overror eyep e.e tub-A

alednnqpapc 100'rreer5
lo,rl nev.raqer;T03A:250v

!!

onq

ir.

h;oqen

lamp

and toolt

lorrhe inlegraled

f.r.hanqnq ihepholoube

lor

attach

.e.ommended
lowbiqhtne$ ev. n orderro

f or.o!-{inq unused

lorapp

io'. ean'nq opr

e odr.aloveroad

nq.o orcd-drk.ondenser

(aronr

Lum'na1on

lorie.hn

utng mme6on oi

ra

ques

wth a

nn,ument

,uda.P!

protecion 1., rhe

!fthe

t

t
t
t
t
t
t
t

I
I

t

.l

I

,|

nlerte,en.e
ntederence band

lT.onvPB.i llter, d = 12:2

CB
cB 6.oive6onJ ter d = 32
CB 3.onvetr on

3200.5500
N 025 newa den5lvtiiP, d=17x2
N 0 06 neDta

KG I h€at
Ref e.i'.n

Dun coler G

rv

de band

llte,

K.oiv€6 on riler, d=32:

-densty

prore.l

pDtectoi

heal

lony

ir ie,,9,een

q,een

fi1er,

d=12!2

l

t'1er, d

on

i.ombinati.n !v th

546,

x2

ter d = r2 x 2

=

32 r 2

I her, d = l2

to,

enlran.n9lrie.o.tan

phdidq,aPhY

lo, ro or
ro or n ms and artil.b

1or

obsetual

rvih tansm n on nformation

phoroq,aphyuthout.o

for

pr.1ed

for

ior .over nq the innrumenl

and

Phase.on1Gn

photoqraphy

on and b/wphorogcphy

nq s.nstive spe.,mens

n b^v

us nq day

qht

coo,

in

or

when is

qht

!n

lrom

I
I

'l
'l

I
I
I
I
I

I

a

I
'l

I

I

t

Care, Ma ntenan.e, TroubLeshmnng

Requ$ling

t

seruice

t
t

'r

_

t

t

t
t

-

t

I

­t
"
t

t'

4i
All repaic ol mechanical, optical or elecrronic

components nsde the nsrrumed and ol lh-"
eleclr ca @mpo.ents of rhe a. ostar may only

pedormed

be
specranv 6utnonzed

To

ensur€ the opumum s( ng a.d troub eiree

iunction of rcur midoscooe ow a lonoer

pe

od ol iim;, wewould rc;ommend $at

con.lude a ,eryi.smainrenance codrac( wrrh

In

the case of subsequent ordeE or when

setoice

your

ocal Cad 2eis agency.

,unn",

ntehet

!v!w.ze $.de/mi(o

Requ€dlngsetul@

by Car Zeiss 5eBc€ statf or

petsonf

is

requned,

i.tormaton is

und€riheloll@ing addre$:

please qet

e

n touch with

also ava

lable

v;!

n the

I

t

t
t
I

I

t

a
a

t

t

I

I

I

|

840-011€

l2l00

4-9

C.r€, M a inlenance,

Request

-Iou

ng sedi.e

blesh oding

ii

.l

':

a.{.

";

.{

,',,1

,q

'4

't

'-,i
',rI

,.i

,:a
,a

B 40431 €

I2/00

,.

,l
,I
,l

I
I

,

'a

t

'a

t
t

,t
t

t

t

,

I
D
D

a

D
D

I
I

,t
t

a

ANNEX

I
I
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App ication

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phototube

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Exchanging lanps........... ............v1, !-4

Eyepiece rercle.................. I 3, 2 4, 3t2

Eyepiece... ....... ....1-3, l-8, l-l1, 2-3, 3 5,3-6

Fi-ald

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Filter..
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amp. .r-3, 1-5, 1r0, 2-r2, 3-:,4-4

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Insirumentsalery.... ........... ..................V

Inrerfercn.e wide-ba.d lilter . .,., .. 1-5,3-3

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Contquraiions and modules.....

ConveEiontits.....

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Darkli€id....

Descrptioo.....................,....

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Doprer.............................,....

Dsposal........................

Doubletube.....................................3

Dlst-protecton

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ga$

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Microscopecamera..............................,..3-16

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Nume.icalapelore.,................15,

objecrivemagnifi.ation..,........................1,6

objecrive.... .... .....,.....1-3,

On/off swirch...........................,.

operation...............................,,.....,.,,.,..Vt.

Opto-m*hanicai data..............................1-11

Overal

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of enCihs........,.,..,.............3-r2

equipm€nr...........,.......3-14

1-6, 1-7. r-t 1, 2-3,

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vieiiganqle....................................... I 11

Viewiiq heiqhr.......................... l-3, 1-11, 2-6

Warunt......................,....,,.,.....,..,....,.,..,..,V||

Wea ng

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Phase nop...... .. .. .. .. .. .. ..,.....,.

Polarizato. conr6 ........................,,....,..,3-r0

Pola aton iiier... ..... ..... ........,,....

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seryice......................................................4-9

5LR cameG..........................,..,..,............3-14

Spares..................................,..,..,..............48

5pecimen holder..........,..,.........................2-6

Stagemicromerer .................,...................1€

5tand...,....,.....................,,....,..............1-!,

5tad-up..................................,..,..,...........2-2

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