Zeiss AxioVision User Manual

The “How Do…” Guide

to AxioVision

Author:

Brian Svedberg

Image Analysis Specialist

Carl Zeiss MicroImaging, Inc.

How do I grab a single image?

How do I grab a single image?

• Click on the Camera icon to switch to the camera property page.

• Make sure light is directed to the camera, and click the Live icon .

• Click the Measurement icon . The exposure will automatically

adjust.

• Fine tune the exposure by adjusting the exposure slider or using the up and down
arrows.

• In the case of a color camera click the Interactive button, which will change to
picking... and click on an area that’s white in the live image.

Æ

• Click the Snap icon to capture the image.

Carl Zeiss The "How Do..." Guide to AxioVision - 02.2002 1

How do I use the archive?

How do I use the archive?

• To create a new archive go to the menu at the top and go to File Æ New and
the New dialog box will open.

• Go to the Archives tab.

• Enter a name for your archive in the space provided on the right and click OK.

• To open an existing archive click on the Open Archive icon .

• To add an image to the archive make sure the image you want to add is active

and click the Add Active Image to Archive icon

.

• Add any information you would like to store with the image into the archive form
and click OK.

Carl Zeiss The "How Do..." Guide to AxioVision - 02.2002 2

How do I create a template for multichannel images?

How do I create a template for multichannel
images?

• Go to File Æ New and the New dialog box will open up.

• Go to the Images tab.

• Select the Multichannel template and click OK.

• A blank window will open with the word Template in the top left.

• Proceed to the Acquisition page by clicking on the Acquisition icon .

• Click on the Color tab. There are three default channels (DAPI, FITC, Rhodamine).

Carl Zeiss The "How Do..." Guide to AxioVision - 02.2002 3

How do I create a template for multichannel images?

• Click on the Add and Remove buttons to get to the number of channels you
want for your template.

• To change the filters click on the Color column and select from the list provided
or go to Edit Custom Colors to create your own.

• To change the exposure time for each channel highlight the current time and
enter a new one. Hit Tab on the keyboard for the change to take effect.

Carl Zeiss The "How Do..." Guide to AxioVision - 02.2002 4

How do I create a template for multichannel images?

• If you have a motorized microscope you can save a setting with each channel. (To
create microscope setting see page 17).

• Highlight the first channel by click on the gray square to the left of the enable
column.

• Go to the Microscope Settings area at the bottom of the page and choose the
setting corresponding to the highlighted channel from the pull-down menu under
During Acquisition.

• Do the same for under Between Acquisition (usually Shutter closed).

• Repeat this for each channel.

• Once you’ve set the filter, exposure, and microscope setting (if applicable) go to

File- Æ Save as Template and enter a name (i.e. DAPI&FITC&Rhodamine) and
click OK.

Carl Zeiss The "How Do..." Guide to AxioVision - 02.2002 5

How do I use a template to grab a multichannel image?

How do I use a template to grab a multichannel
image?

• Open your template for the image you would like to grab by going to File Æ

New or clicking on the New icon .

• The New dialog box will open with a list of all templates. Highlight the template
you want to use by clicking on it once, and enter a name for your image in the
space provided on the right side. Click OK.

• The blank template window will open.

• Go to the Acquisition page by clicking on the Acquisition icon .

• Click on the Color tab.

• Highlight the first channel by clicking on the gray box to the left of the enable

column.

• Make sure the microscope is set-up correctly for the corresponding channel and
light is directed to the camera.

Carl Zeiss The "How Do..." Guide to AxioVision - 02.2002 6