Zeiss Axiovert 200 M, Axiovert 200 User Manual
Operating Manual
Axiovert 200 / Axiovert 200 M
Inverted Microscopes
INTRODUCTION
Carl Zeiss Copyright Axiovert 200
Knowledge of this manual is required for the operation of the instrument. Would you please therefore
make yourself familiar with the contents of this manual and pay special at tent ion to hints concerning t he
safe operation of the instrument.
The specifications are subject to change; the manual is not covered by an update service.
© Unless expressly authorized, forw arding and duplication of this document, and the utilization and
communication of its contents are not permitted. Violations will entail an obligation to pay
compensation.
All rights reserved in the event of gr anting of patents or registrat ion of a utility model.
Issued by: Carl Zeiss
Light Microscopy
P.O.B. 4041
37030 Göttingen
GERMANY
Phone : ++49 551 5060 660
Telefax: ++49 551 5060 464
Internet : www.zeiss.de/micro
E-Mail: micro@zeiss.de
Number of this manual: B 40-080 e
Date of issue: 30.03.2001
0-2 B 40-080 e 03/01
INTRODUCTION
Axiovert 200 Contents Carl Zeiss
CONTENTS
page
INTRODUCTION........................................................................................................0-2
Copyright ...................................................................................................................0-2
Contents.....................................................................................................................0-3
Notes on instrument safety..........................................................................................0-7
Notes on warranty....................................................................................................0-10
Overall view of the Axiovert 200................................................................................0-11
Overall view of the Axiovert 200 M............................................................................0-11
Microscopy in transmitted-light brightfield in a few steps...........................................0-12
CHAPTER 1 INSTRUMENT DESCRIPTION .....................................................................................1-3
1.1 Name and intended application...................................................................................1-3
1.2 Instrument description and main features....................................................................1-5
1.3 Microscope configurations and modules......................................................................1-6
1.4 Objectives.................................................................................................................1-10
1.5 Eyepieces..................................................................................................................1-12
1.6 Condensers...............................................................................................................1-12
1.7 Specimen stages and mounting frames......................................................................1-13
1.8 Binocular tubes.........................................................................................................1-13
1.9 Technical Data..........................................................................................................1-14
B 40-080 e 03/01 0-3
INTRODUCTION
Carl Zeiss Contents Axiovert 200
page
CHAPTER 2 START-UP.................................................................................................................. 2-5
2.1 Unpacking and installation of the microscope............................................................. 2-5
2.2 Attachment of binocular (photo) tube.........................................................................2-6
2.2.1 Inserting the eyepieces and the centering telescope.................................................... 2-6
2.2.2 Inserting the eyepiece reticle....................................................................................... 2-7
2.3 Attachment of transmitted-light illumination............................................................... 2-8
2.3.1 Attachment of carrier for transmitted-light illumination (100 W).................................. 2-8
2.3.2 Attachment of carrier for transmitted-light illumination (30 W).................................... 2-8
2.4 Screw in objectives .....................................................................................................2-9
2.5 Attachment of microscope stages ............................................................................. 2-10
2.5.1 Attachment of mechanical stage 130x85 R/ L and m ount ing fr a m e
for mechanical stage (K)............................................................................................2-10
2.5.2 Attachment of scanning stage...................................................................................2-10
2.5.3 Attachment of specimen st age 250x230, object guide and m ount ing f rame
for object guide (M).................................................................................................. 2-11
2.5.4 Attachment of heating stage ....................................................................................2-12
2.5.5 Attachment of gliding stage Z................................................................................... 2-12
2.6 Attachment of condensers........................................................................................2-13
2.6.1 Condensers for the Axiovert 200............................................................................... 2-13
2.6.2 Condensers from the Axioplan 2 imaging / Axioskop 2 product line........................... 2-13
2.6.3 Changing the DIC prism in the condenser turret........................................................ 2-14
2.7 Reflector turret.........................................................................................................2-15
2.7.1 Attachment of reflector turret................................................................................... 2-15
2.7.2 Equipment of reflector turret ....................................................................................2-15
2.7.3 Changing the filter set in the FL reflector module...................................................... 2-16
2.7.4 Changing the beam splitter in the FL reflector module ............................................... 2-16
2.8 Connection to the line.............................................................................................. 2-18
2.9 Interfaces of the Axiovert 200 M............................................................................... 2-19
2.10 Switch microscope and ebq 100 dc power supply on and off.....................................2-19
2.11 Equipotential bonding terminals................................................................................ 2-19
2.12 HAL 100 halogen illuminator..................................................................................... 2-20
2.12.1 Change / attachment of the HAL 100 halogen lamp.................................................. 2-20
2.12.2 Coarse alignment of halogen illuminator................................................................... 2-21
2.12.3 Attachment of halogen illuminator............................................................................ 2-21
2.12.4 Fine alignment of halogen illuminator .......................................................................2-22
0-4 B 40-080 e 03/01
INTRODUCTION
Axiovert 200 Contents Carl Zeiss
page
2.13 Illuminator N HBO 103..............................................................................................2-23
2.13.1 Attachment / change of the HBO 103 W/2 mercury pressure short-arc lamp...............2-23
2.13.2 Coarse alignment of illuminator N HBO 103...............................................................2-25
2.13.3 Attachment of illuminator N HBO 103.......................................................................2-26
2.13.4 Fine adjustment of illuminator N HBO 103 using the adjusting aid..............................2-27
2.14 Attachment of Aqua Stop .........................................................................................2-28
CHAPTER 3 OPERATION...............................................................................................................3-3
3.1 Axiovert 200 (manual).................................................................................................3-4
3.1.1 Operation and function controls on the Axiovert 200 (manual).....................................3-4
3.1.2 Switching on and basic settings on the Axiovert 200 (manual)....................................3-14
3.2 Axiovert 200 M (motorized).......................................................................................3-16
3.2.1 Operation and function controls on the Axiovert 200 (motorized)...............................3-16
3.2.2 Switching on and basic settings on the Axiovert 200 M (motorized)...........................3-23
3.3 Illumination and contrasting techniques.....................................................................3-28
3.3.1 Setting of transmitted-light brightfield for KÖHLER illumination..................................3-28
3.3.2 Setting of transmitted-light phase contrast................................................................3-33
3.3.3 Setting of differential interference contrast (DIC) in transmitted light..........................3-35
3.3.4 Setting of VAREL contrast in transmitted light............................................................3-38
3.3.5 Setting of fluorescence contrast in reflected light.......................................................3-40
3.4 Documentation.........................................................................................................3-43
3.4.1 Image orientation of camera ports.............................................................................3-43
3.4.2 Photomicrography with SLR camera...........................................................................3-45
3.4.3 Photomicrography using a digital camera and videomicroscopy..................................3-46
B 40-080 e 03/01 0-5
INTRODUCTION
Carl Zeiss Contents Axiovert 200
page
CHAPTER 4 CARE, MAINTENANCE, TROUBLESHOOTING AND SERVICE.................................... 4-3
4.1 Care........................................................................................................................... 4-3
4.2 Maintenance..............................................................................................................4-4
4.2.1 Performing checks...................................................................................................... 4-4
4.2.2 Changing the fuses on the microscope........................................................................ 4-4
4.2.3 Changing the fuses on the ebq 100 dc power supply ..................................................4-5
4.3 Service........................................................................................................................4-6
ANNEX......................................................................................................................A-1
List of abbreviations....................................................................................................A-3
List of key words.........................................................................................................A-5
Certification in accordance with DIN ISO 9001 / EN 46001
EC Declaration of Conformity
0-6 B 40-080 e 03/01
INTRODUCTION
Axiovert 200 Notes on instrument safety Carl Zeiss
Notes on instrument safety
The Axiovert 200 / Axiovert 200 M microscopes were designed, produced and tested in compliance with
DIN 61010-1 (IEC 1010-1), Safety requirements for electrical measuring, control and laboratory
instruments, and meet the requirem ent s of appendix I of directive 73/23/EC and the relevant CSA and UL
directives. The instruments meet the requir ements of the EC direct ive 89/336/EC and the EMC legislation
of November 9th 1992. This operation manual includes information and warnings which must be
observed by the user.
The following warning and information symbols are used in this ma nual:
☞
NOTE
This symbol is a warning which you must observe under all circumstances.
CAUTION
This symbol is a warning which indicates a hazard to the instrument or instrument system.
CAUTION
This symbol is a warning which indicates a hazard to the user of the instr ume nt .
CAUTION
Hot surface!
CAUTION
UV radiation is emitted!
CAUTION
Disconnect the instrument from the line before opening it!
B 40-080 e 03/01 0-7
INTRODUCTION
Carl Zeiss Notes on instrument safety Axiovert 200
The Axiovert 200 microscopes, including original accessories, may only be used for the microscope
techniques described in this manual.
Particular attention must be paid to the following notes:
The manufacturer cannot assume any liability for any other applications, possibly also involving
individual modules or single parts. This also applies to all service or repair work which is not
carried out by authorized service personnel. Furthermore, this forfeits all the claims against
warranty
The power plug must be inserted in a socket featuring a grounding (earth) contact. The
grounding effect must not be made ineffective by an extension cable which does not have a
protective ground wire.
If it is established that the protection measures are no longer effective, the instrument must be
switched off and safeguarded against inadvertent operation. For repair of the instrument,
contact the Carl Zeiss microscope service in Germany (see page 4-6) or your local Carl Zeiss
agency.
The Axiovert 200 microscopes are not equipped with any special devices for protect ion from
substances which are corrosive, toxic, radioactive or otherwise hazardous to health. All the
legal regulations for accident prevention, particularly those in the respective countries, must be
observed when handling such substances.
Before switching on the power unit for t he HBO 50/100, check whether it is suitable for the
line voltage present.
Always disconnect the instrument from the line before opening the instrument and before
changing the fuses.
Make sure to use only fuses of the rated power required. The use of makeshift fuses and the
short-circuiting of the fuse holders are not permitted.
0-8 B 40-080 e 03/01
INTRODUCTION
Axiovert 200 Notes on instrument safety Carl Zeiss
Gas discharge lamps, e.g. HBO 100, emit ultraviolet radiation which can cause burns on the
eyes and skin. Therefore, never look directly into the light of these lamps and avoid direct,
unprotected incidence of their light on your skin. When using the microscope, always use the
protective devices belonging to the instrument (e.g. special attenuat ion filters). When hot, gas
discharge lamps are under high internal pressure and must therefor e be changed when cooled
down by using protective gloves and goggles (for detailed information, please see the
operating instructions B 40-065 e).
When fluorescence filters are used, the filter protecting from heat emitted by the microscope
illuminator must not be removed, since fluorescence filters are sensitive to heat and their
function might therefore be impaired.
Placing objects against or covering ventilation slats can lead to a build-up of heat which will
damage the instrument and, in extreme cases, cause a fire. Always keep the ventilation slats
clear and make sure that no objects enter the instrument through the ventilation slats.
Avoid touching the hot lamp housings. Always pull the power plug befor e changing the lamps
and allow the instrument to cool down for approx. 15 mins.
Dust and dirt can impair the performance of the instrument. Therefore, the instrument must be
protected against these influences as far as possible, and covered with the dust cover if it is not
used for longer periods of time. Always check whether the instrument is switched off before
you cover it.
The instruments may only be operated by trained personnel who m ust be aw ar e of the possible
danger involved with microscopy and the relevant application.
The Axiovert 200 microscopes are opto-mechanical precision instruments which can be
impaired in their performance or damaged when handled improperly.
B 40-080 e 03/01 0-9
INTRODUCTION
Carl Zeiss Notes on warranty Axiovert 200
Notes on warranty
The manufacturer guarantees that the instrument has no material and production defects when
delivered. You must inform us of any defects immediately and we must do anything to minimize the
damage. If the manufacturer is informed of such a defect, he is obliged to remove it; it is his decision
whether he does this by repairing the instrument or by deliver ing an instrument free of any defect. No
guarantee is provided for defects caused by natural wear ( w ear ing par t s in par t icular) and im proper use.
The instrument manufacturer is not liable for damage caused by faulty operation, negligence or any
other meddling with the instrument, particularly the removal or replacement of instrument components,
or the use of accessories from other manufacturers. This forfeits all the claims against warranty.
With the exception of the work specified in this manual, no maintenance or repair of the microscopes
may be performed. Repairs may only be performed by Carl Zeiss service staff or specially authorized
personnel. Should any defect occur with the instrument, please get in touch with the Carl Zeiss
microscopy service in Germany (see page 4-6) or your local Zeiss agency.
0-10 B 40-080 e 03/01
INTRODUCTION
Axiovert 200 Overall view of the Axiovert 200 and Axiovert 200 M Carl Zeiss
B 40-080 e 03/01 0-11
INTRODUCTION
Carl Zeiss Microscopy in transmitted-light brightfield in a few st eps Axiovert 200
Microscopy in transmitted-light brightfield in a few steps
☞
• Make the microscope ready for operation as described in chapter 2 and switch it on via the On/Off
switch (0-2/1).
• Select the objective with the lowest magnification (e.g. 10x) on the nosepiece (0-2/2). Set factor 1x on
the setting wheel (0-2/4) of the Optovar turret.
• Open the luminous-field diaphragm or the aperture diaphr agm completely by pulling lever (0-2/16) to
the front until stop or by turning the sett ing w heel ( 0- 2/ 20) t o t he front until stop.
• Turn the setting ring (0-2/19) to move the condenser turret in position H for brightfield (or DIC).
• Move reflector turret (0-2/5, if available) into the position without filter combination via the setting
ring.
• If required, remove analyzer slider (0-2/3) or switch to free light path.
• Turn setting wheel for Sideport right / left / vis (0-2/22) to position 100 % vis (visual
• Turn setting knob for Frontport / Baseport / vis (0- 2/ 23) t o posit ion 100 % vis (
• Set beam splitting ratio to 100 % vis (0-2/10) on the tube. Switch off the Bertrand lens (if available).
Move combined rotary / slider knob (0-2/9) to posit ion 100 % vis (
• Place a high-contrast specimen on the microscope stage (0-2/21). Adjust the binocular component.
Before starting to use the Axiovert 200, make sure to read the notes on instrument safety and
the chapters entitled "Instrument Description" (Chapter 1) and "Start-up" (Chapter 2).
).
VIS
).
).
• Use the coarse / fine focusing drive (0-2/6) to focus on the selected detail of the specim en. Should no
light be visible in the eyepieces, switch on t he halogen illum inat or v ia th e HAL on / off switch (0-2/7).
• Use the toggle switch (0-2/8) to set the light
intensity to comfortable brightness.
• Close luminous-field diaphragm (0-2/16) until it
is visible in the field of view, even if not in focus
Fig. 0-1 Diaphragm settings in transmitted-
light brightfield according to
KÖHLER
• Center (0-1/C) luminous-field diaphragm via the centering screws ( 0-2/15 and 18) and open it until
the edge of the diaphragm just disappears from the field of view ( 0- 1/D).
• Remove one eyepiece from the eyepiece tube (or swing in Bertrand lens) and set aperture diaphragm
(0-2/20) to approx. 2/3 of the diameter of the objective exit pupil ( 0-1/E). Optimum contrast setting is
dependent on the respective specimen.
• Insert the eyepiece again (or swing out Bertrand lens) and refocus, if required, via the fine drive.
• After the microscope has been set to transmitted-light brightfield in this way, changing to this special
contrasting technique is now possible (see chapter 3 of this m anual).
(0-1/A).
• Focus on the edge of the luminous-field
diaphragm (0-1/B) by moving the condenser
(0-2/17) vertically.
0-12 B 40-080 e 03/01
INTRODUCTION
Axiovert 200 Microscopy in transmitted-light brightfield in a few steps Carl Zeiss
1 On / Off switch
2 Objective nosepiece
3 Compartment for slider
Analyzer
4 Setting wheel for Optovar turret
5 Reflector turret
6 Focusing drive coarse / fine
7 HAL on / off switch
8 Toggle switch for illumination
intensity
9 Turning or sliding knob for
vis / doc beam splitting
10 Turning or sliding knob for
Bertrand lens and manual shutter
11 Binocular tube component
12 Setting ring of the eyepiece
13 Eyepiece
14 Polarizer D with 2-position filter
changer
15 Centering screw for condenser
16 Setting lever for luminous-field
diaphragm
17 Setting knob for vertical
adjustment of the condenser
18 Centering screw for condenser
19 Turret disk of condenser
20 Setting wheel for aperture
diaphragm on the condenser
21 Microscope stage
22 Compartment for aperture
diaphragm slider
23 Setting wheel for Sideport
24 Setting knob for Frontport /
Baseport
Fig. 0-2 Axiovert 200
B 40-080 e 03/01 0-13
INTRODUCTION
Carl Zeiss Microscopy in transmitted-light brightfield in a few st eps Axiovert 200
0-14 B 40-080 e 03/01
INSTRUMENT DESCRIPTION
Axiovert 200 Contents / List of illustra t ions Carl Zeiss
INSTRUMENT DESCRIPTION
Contents
1 INSTRUMENT DESCRIPTION..........................................................................................1-3
1.1 Name and intended application........................................................................................1-3
1.2 Instrument description and main features.........................................................................1-5
1.3 Microscope configurations and modules........................................................................... 1-6
1.4 Objectives......................................................................................................................1-10
1.5 Eyepieces.......................................................................................................................1-12
1.6 Condensers...................................................................................................................1-12
1.7 Specimen stages and mounting frames..........................................................................1-13
1.8 Binocular tubes............................................................................................................. .1-13
1.9 Technical Data .............................................................................................................. .1-14
List of illustrations
Fig. 1-1 Microscope configurations and modules (sheet 1) ............................................................1-6
Fig. 1-2 Microscope configurations and modules (sheet 2) ............................................................1-7
Fig. 1-3 Microscope configurations and modules (sheet 3) ............................................................1-8
Fig. 1-4 Microscope configurations and modules (sheet 4) ............................................................1-9
B 40-080 e 03/01 1-1
INSTRUMENT DESCRIPTION
Carl Zeiss Contents / List of illustrations Axiovert 200
1-2 B 40-080 e 03/01
INSTRUMENT DESCRIPTION
Axiovert 200 Name and intended application Carl Zeiss
1 INSTRUMENT DESCRIPTION
1.1 Name and intended application
Manufacturer's name: Inverted microscope for transmitted light and epifluorescence
Brief name: Axiovert 200 (manual version)
Axiovert 200 M (motorized version)
The Axiovert 200 microscopes fit in the product family of inverted transmitted-light microscopes as
follows:
Laboratory microscopes Research microscopes
− Axiovert 25
− Axiovert 25 C
− Axiovert 25 CFL
The Axiovert 200 microscopes are universally applicable inverted microscopes and are m ainly used for the
examination of cell and tissue cultures and of sediments in culture flasks, Petri dishes, microtiter plates,
etc. in transmitted and reflected light.
The Axiovert 200 microscopes permit the performance of the transmitted-light techniques brightfield,
phase contrast, differential interference contrast and VAREL contrast, and the epi-fluorescence
technique.
The microscopes Axiovert 200 and Axiovert 200 M are the basis for scientific microscopic work on living
cells.
− The sturdy stand provides attac hment possibilities for various t ools ( micr omanipulat ion), diff erent light
sources, temperature control devices, etc.
− Ample space is available for the specimens and the relevant handling systems on account of the
inverted design, the LD illumination system of the microscope and the use of fixed stages. This
permits the performance of experiments which w ould not be possible w ith upr ight m icroscopes.
− The design allows the easy attachment of cameras, lasers, specific stages, etc.
− Axiovert 200
− Axiovert 200 M
B 40-080 e 03/01 1-3
INSTRUMENT DESCRIPTION
Carl Zeiss Name and intended application Axiovert 200
Typical fields of application:
Observation of intracellular processes in living cell cultures, cell/cell interactions, motility, growth,
measurements of potential, drug detection, microinjection, IVF (in-vitro fertilization), toxicity
examinations, patch-clamp technique, ion measurements, digital recording, long-time / time lapse
examinations in combination with the automation of processes, z-sectioning, deconvolution, visualization
of molecular structures, Fura (Ca measurement), GFP, optical tweezers and scissors, single molecule
detection, TIRF ....
Accessories for temperature control and incubation are described in the separate manual B 40- 610 d/e,
"Incubation systems on the Axiovert".
1-4 B 40-080 e 03/01
INSTRUMENT DESCRIPTION
Axiovert 200 Instrument description and main features Carl Zeiss
1.2 Instrument description and main features
The Axiovert 200 / Axiovert 200 M is available either as manual or as motorized version. The accessory
components are part of a modular system.
For documentation purposes, the Axiovert 200 / Axiovert 200 M can be equipped with the maximum of
five camera / TV ports in accordance with the customer's requests.
Adaptation possibilities are provided for heating s t ages , inc ubat or s and m ic r om anipulat ors.
Major instrument features:
− ICS optics for image creation
− high thermal and mechanical stability
− high flexibility in documentation
− improved ergonomic design
− LCD display of instrument parameters
− 23 mm field of view
− Light Manager
− modular design for optimum adaptation to the r e levant applicat ion
− 6-position nosepiece, coded
− 5-position reflector turret, manual: can be changed on both sides; motorized: can be changed from
the right
− 5-position or 6-position condenser turret
− 3-position Optovar turret
− changeable aperture diaphragm and luminous-field diaphragm sliders in ref lect ed light
− fluorescence shutter
− illuminators: HAL 100 W, HBO 50, N HBO 103, N XBO 75
− Axiovert 200 M, all the major microscope functions are motorized.
B 40-080 e 03/01 1-5
INSTRUMENT DESCRIPTION
Carl Zeiss Microscope configurations and modules Axiovert 200
1.3 Microscope configurations and modules
Fig. 1-1 Microscope configurations and modules (sheet 1)
1-6 B 40-080 e 03/01
INSTRUMENT DESCRIPTION
Axiovert 200 Microscope configurations and modules Carl Zeiss
Fig. 1-2 Microscope configurations and modules (sheet 2)
B 40-080 e 03/01 1-7
INSTRUMENT DESCRIPTION
Carl Zeiss Microscope configurations and modules Axiovert 200
Fig. 1-3 Microscope configurations and modules (sheet 3)
1-8 B 40-080 e 03/01
INSTRUMENT DESCRIPTION
Axiovert 200 Microscope configurations and modules Carl Zeiss
Fig. 1-4 Microscope configurations and modules (sheet 4)
B 40-080 e 03/01 1-9
INSTRUMENT DESCRIPTION
Carl Zeiss Objectives Axiovert 200
1.4 Objectives
The objectives are the optical centerpiece of the microscope. The following is an example of how
objectives can be labelled:
A-Plan 10×/0.20 HD ∞/-
Key:
10× objective magnification, with a color ring on the objective being allocat ed to each m agnification
step (Zeiss color code)
0.20 numerical aperture
HD reflected-light brightfield and darkfield objective
∞ infinite tube length
- can be used without cover slip (D = 0 mm) or with cover slip thickness D = 0. 17 m m
or
0 can only be used without cover slip (D = 0 mm)
0.17 can only be used with cover slip thickness D = 0.17 mm
and
Oil oil immersion objective
Ph 2 phase contrast object ive w ith a green color r ing and phase stop Ph 2
Color ring code for objective magnification:
Color ring on
objective
Magnification
factor
black brown red orange yellow green light
blue
1.25× 2.5× 4×; 5× 6.3× 10× 16×;20×;
25×; 32×
40×; 50× 63× 100×;
dark
blue
white
150×
The objective magnification (e.g. 10x) multiplied with the eyepiece magnification (e.g. 10x) and the
Optovar magnification (e.g. 1.6x) results in the visual overall magnification;
example: 10 x 10 x 1.6 = 160x.
The numerical aperture x 1000, e.g 0.20 x 1000 = 200x, is the highest useful magnification, i.e. no
further details are resolved above that limit.
In transmitted-light applications, the exact observance of the cover slip thickness of 0.17 mm is all the
more necessary the higher the numeric aperture of the objective. Therefore, so-called "corr" objectives
can be set for different cover slip thicknesses via a correction ring. For this, a specimen area is searched,
and the position of the correction ring where optimum focus and image contrast are obtained is
determined (refocusing is always required).
Immersion objectives are always insensitive to differences in cover slip thickness.
When immersion objectives are used, the air between the cover slip and the objective is replaced with a
liquid, which is immersion oil in most cases.
1-10 B 40-080 e 03/01
INSTRUMENT DESCRIPTION
Axiovert 200 Objectives Carl Zeiss
The following objectives are available for the Axiovert 200 / Axiovert 200 M microscope:
Objective type Magnification/
numeric aperture
A-Plan
A-Plan 5x/0.12 a = 9.1 ph0 000000-1018-589
A-Plan 10x/0.20 a = 4.4 ph1 var1 000000-1020-863
LD A-Plan 20x/0.30 D = 0.6 - 1.4 a = 4.3 ph1 000000-1006-591
LD A-Plan 20x/0.30 D = 0.6 - 1.4 a = 4.2 ph1 var1 000000-1006-592
LD A-Plan 32x/0.40 D = 0.6 - 1.4 a = 3.1 ph1 000000-1006-593
LD A-Plan 32x/0.35 D = 0.6 - 1.4 a = 3.1 ph1 var1 000000-1006-594
LD A-Plan 40x/0.50 D = 0.7 - 1.3 a = 2.3 ph2 000000-1006-595
LD A-Plan 40x/0.50 D = 0.7 - 1.3 a = 2.3 ph2 var2 000000-1006-596
Cover slip cap D = 0.17 - 0.6 000000-1016-757
LD Achroplan *
Achroplan 4x/0.10 - a = 11.1 440020-0000-000
Achroplan 10x/0.25 - a = 4.8 ph1 440031-0000-000
LD Achroplan 20x/0.40 corr D = 0 - 1.5 a = 10.2 440844-0000-000
LD Achroplan 20x/0.40 corr D = 0 - 1.5 a = 10.2 ph2 440845-0000-000
LD Achroplan 40x/0.60 corr D = 0 - 2 a = 1.8 440864-0000-000
LD Achroplan 40x/0.60 corr D = 0 - 2 a = 1.8 ph2 440865-0000-000
LD Achroplan 63x/0.75 corr D = 0 - 1.5 a = 1.57 ph2 440861-0000-000
Plan-Neofluar
Plan-Neofluar 5x/0.15 - a = 13.6 440320-0000-000
Plan-Neofluar 5x/0.15 - a = 13.6 ph1 440321-0000-000
Plan-Neofluar 10x/0.30 D = 0.17 a = 5.6 440330-0000-000
Plan-Neofluar 10x/0.30 D = 0.17 a = 5.6 ph1 440331-0000-000
Plan-Neofluar 16x/0.50 Imm D = 0.17 a = 0.22 440530-0000-000
Plan-Neofluar 16x/0.50 Imm D = 0.17 a = 0.22 Ph1 440531-0000-000
Plan-Neofluar 20x/0.50 D = 0.17 a = 2.0 000000-1004-072
Plan-Neofluar 20x/0.50 D = 0.17 a = 2.0 ph2 000000-1004-989
Plan-Neofluar 40x/0.75 D = 0.17 a = 0.5 440350-9902-000
Plan-Neofluar 40x/0.75 D = 0.17 a = 0.5 ph2 440351-9902-000
Plan-Neofluar 40x/1.30 Oil D = 0.17 a = 0.2 000000-1022-818
Plan-Neofluar 40x/1.30 Oil D = 0.17 a = 0.2 ph3 000000-1022-819
Plan-Neofluar 63x/0.95 Korr. D = 0.17 a = 0.12 440364-0000-000
Plan-Neofluar 100x/1.30 Oil D = 0.17 a = 0.12 000000-1018-595
Plan-Neofluar 100x/1.30 Oil D = 0.17 a = 0.12 ph3 000000-1031-171
Cover slip
thickness D in
mm
Free working
distance A in
mm
Contrasting Cat. No.
Plan-Apochromat
Plan-Apochromat 63x/1.40 Oil D = 0.17 440760-0000-000
* a refers to d = 1
B 40-080 e 03/01 1-11
INSTRUMENT DESCRIPTION
Carl Zeiss Eyepieces / Condensers Axiovert 200
1.5 Eyepieces
The following eyepieces are available for the Axiovert 200 and Axiovert 200 M:
Eyepiece type Image angle Cat. No.
Eyepiece W-PL 10×/23 Br. foc.
Eyepiece W-PL 10×/23 Br. foc.
24.7° 455043-0000-000
24.7° 000000-1016-758
Centering telescope d = 30 000000-1006-362
1.6 Condensers
The following condensers are available for combination with the stage carrier D and the transmitted-light
equipment on the Axiovert 200 / Axiovert 200 M:
Condenser type Cat. No. Comments
LD condenser 0.35, 5 positions:
000000-1005-844 from object ive 2.5x
H/DIC, Ph0, Ph1, Ph2, Var1/2
LD condenser 0.55, 6 positions:
000000-1005-848 from object ive 4x
H, Ph1, Ph2, Ph3, DIC, DIC mot.
Condenser 0.35, 6 positions:
000000-1005-845
H/DIC, Ph0, Ph1, Ph2, DIC, DIC
Condenser 0.55, 5 positions:
000000-1005-846
H, Ph1, Ph2, Ph3, Var1/2
Condenser 0.55, 6 positions:
000000-1005-847
H, Ph1, Ph2, Ph3, DIC, DIC
Condenser adapter for condensers 0.8
000000-1005-849
and 1.4
Condenser 0.8 H, D 0.66 / 0.8 Ph DIC,
plus factory-aligned: DIC pris m III/0.8
Condenser 0.8 H, DIC, plus factory-
aligned: DIC prism III/0.8
Condenser 1.4, 6 positions:
445445-9901-000
445485-0000-000
000000-1087-444
000000-1087-445
445453-0000-000
H, D, Ph 2x, DIC 2x
1-12 B 40-080 e 03/01
INSTRUMENT DESCRIPTION
Axiovert 200 Specimen stages and mounting frames / Binocular t ubes Carl Zeiss
1.7 Specimen stages and mounting frames
The Axiovert 200 microscopes can be equipped with the following specimen stages and mounting
frames:
Description Cat. No.
Specimen stage 250x230 mm with ceramic surface and
000000-1005-832
3-point support
Object guide 130x85 right, can be attached on both
000000-1005-833
sides, accepts various mounting frames
Object guide 130x85 left, can be attached on both sides,
000000-1110-991
accepts various mounting frames
Mechanical stage 130x85 R/L 000000-1005-834
Mounting frame for object guide (M) see price list
Mounting frame for mechanical stage (K) see price list
Gliding stage Z 471722-0000-000
Scanning stage 000000-1113-509
1.8 Binocular tubes
The following tubes can be used with the Axiovert 200 microscopes:
Description Cat. No. VIEWING ANGLE
/
Light distribution in
%
FIELD
NUMB
ER
Binocular tube 45°/23 000000-1005-827 45° / 23 100 %
Binocular phototube 45°/23,
000000-1005-828 45° / 23 100-0, 0-100, 50-50
with Bertrand lens and shutter
Binocular ergotube 25°/23
000000-1005-829 25° / 23 100 %
vertically variable,
with Bertrand lens and shutter
B 40-080 e 03/01 1-13
INSTRUMENT DESCRIPTION
Carl Zeiss Technical Data Axiovert 200
1.9 Technical Data
Dimensions (width x depth x height)
Stand Axiovert 200.................................................................................... approx. 295 × 805 × 707 mm
Weight
Axiovert 200 without fluorescence equipment ................................................................... approx. 26 kg
Axiovert 200 M with fluorescence equipment .................................................................... approx. 40 kg
Ambient conditions
Storage and transport (in packaging):
Permissible ambient temperature................................................................................... -40 to +50 °C
Operation:
Permissible ambient temperature.................................................................................. +10 to +35 °C
Permissible relative humidity (without condensation)........................................................... max. 80 %
Altitude of use...............................................................................................................max. 2000 m
Atmospheric pressure......................................................................................... 800 hPa to 1060 hPa
Pollution degree............................................................................................................................... 2
Operating data
Category of use.................................................................................................................. closed rooms
Protection class.......................................................................................................................................I
Protection type................................................................................................................................IP 20
Electrical safety.................................................................in compliance with DIN EN 61010 (IEC 1010-1)
...............................................................................................................including CSA and UL directives
Excess voltage category........................................................................................................ .................II
Radio interference suppression.......................................................in accordance with EN 55011, Class B
Voltage ranges:
Integrated 12 V, 100 W power unit, stabilized ............................................ 100 to 240 VAC (± 10 %)
1-14 B 40-080 e 03/01
INSTRUMENT DESCRIPTION
Axiovert 200 Technical Data Carl Zeiss
Line frequency......................................................................................................................50 to 60 Hz
Power consumption with internal power unit s
Axiovert 200........................................................................................................................... 235 VA
Axiovert 200 M.......................................................................................................................235 VA
and external power units
Power supply N XBO 75, 100 ... 240 V AC...............................................................................130 VA
Power supply N HBO 100, 90 ... 240 V AC...............................................................................265 VA
Fuses in accordance with IEC 127
Axiovert 200
100 ... 240 V AC....................................................................................T 4.0 A/H; 250 V; 5 × 20 mm
Axiovert 200 M
100 ... 240 V AC....................................................................................T 4.0 A/H; 250 V; 5 × 20 mm
Light sources
HBO 50 W/AC mercury pressure short-arc lamp
Lamp voltage for lamp type L1 and L2....................................................L1: 39 ... 45 V/L2: 34 ... 39 V
Power........................................................................................................................................50 W
Average life...............................................................................................................................100 h
HBO 103 mercury vapor short-arc lamp.........................................................................................100 W
Opto-mechanical data
Stand with stage focusing............................................................................ with coarse drive (5 mm/rot)
and fine drive (0.1 mm /rot)
Fine scaling 1 µm/scale part
Overall lift approx. 10 mm
Objective change................................................................................................via 6-position nosepiece
Objectives......................................................................................................with W 0.8" x 1/36"thread
Eyepieces .................................................................................................. with plug-in diameter 30 mm
and............................................................................................................................field number 23
B 40-080 e 03/01 1-15
INSTRUMENT DESCRIPTION
Carl Zeiss Technical Data Axiovert 200
1-16 B 40-080 e 03/01
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