Zeiss Axiostar plus Operating Manual

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Operating Manual

Axiostar

Transmitted-Light Microscope

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Carl Zeiss Copyright Axiostar plus

Knowledge of this manual is required for the operation of the instrument. Therefore please make
yourself familiar with the contents of this manual and pay special attention to hints concerning the
safe operation of the instrument.

The specifications are subject to change; the manual is not covered by an update service.



Unless expressly authorized, forwarding and duplication of this document, and the utilization and

communication of its contents are not permitted. Violations will entail an obligation to pay
compensation.
All rights reserved in the event of granting of patents or registration of a utility model.

Issued by: Carl Zeiss

Light Microscopy

P.O.B. 4041
D-37030 Göttingen
Phone: ++49 551 5060 660
Fax: ++49 551 5060 464
Internet: www.zeiss.de/micro
E-mail: [e-mail]

Number of this manual: B 40-815 e
Date of issue: 12/01

II B 40-815 e 12/01

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Axiostar plus Contents Carl Zeiss

CONTENTS

Page

INTRODUCTION

Title Page............................................................................................................. I

Copyright ........................................................................................................... II

Contents ........................................................................................................... III

General Notes on Safety, Instrument Safety and EMC.........................................VI

Notes on Safety: Unpacking, Transport, Storage, Disposal and operation............ VII

Notes on Safety: Care and Maintenance, Warranty Notes ................................. VIII

1 INSTRUMENT DESCRIPTION ..........................................................................1-3

1.1 Name and intended application........................................................................1-3

1.2 Description and main features..........................................................................1-3

1.3 System overview ..............................................................................................1-4

1.4 Objectives........................................................................................................1-7

1.5 Eyepieces.........................................................................................................1-8

1.6 Stage micrometers and eyepiece reticles...........................................................1-9

1.7 Technical data................................................................................................1-11

2 START-UP.......................................................................................................2-3

2.1 Unpacking the instrument................................................................................2-3

2.2 Attaching and setting ergonomic base plate .....................................................2-3

2.2.1 Attaching the ergonomic base plate ................................................................................2-4

2.2.2 Adjusting the ergonomic base plate.................................................................................2-6

2.3 Attaching tubes ...............................................................................................2-8

2.3.1 Attaching binocular tube 45°/20 ICS................................................................................2-8

2.3.2 Attaching binocular phototube 45°/20 ICS ......................................................................2-8

2.3.3 Attaching binocular tube 30°/20 ICS................................................................................2-9

2.4 Screwing in objectives....................................................................................2-10

2.5 Inserting eyepieces.........................................................................................2-10

2.5.1 Inserting eyepiece reticles ..............................................................................................2-11

2.5.2 Compensation of ametropia when eyepiece reticles are used .......................................2-11

2.5.3 Attaching folding eyecups .............................................................................................2-12

2.6 Setting interpupillary distance and viewing height...........................................2-12

2.7 Setting mechanical stage 75 x 30 R/L..............................................................2-13

2.7.1 Setting drive length on the stage drive...........................................................................2-13

2.7.2 Setting drive knob torque for x/y adjustment on the mechanical stage...........................2-13

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Carl Zeiss Contents Axiostar plus

2.8 Attaching condensers.....................................................................................2-14

2.8.1 Attaching Abbe condenser 0.9/1.25.............................................................................. 2-14

2.8.2 Inserting slider for phase contrast or darkfield.............................................................. 2-14

2.8.3 Attaching Abbe condenser with 5-position turret.......................................................... 2-15

2.8.4 Attaching LED illuminator ............................................................................................. 2-16

2.8.5 Attaching darkfield condenser ...................................................................................... 2-18

2.9 Attaching colored disk carrier, polarizer or wide-angle unit .............................2-20

2.10 Inserting white-balance filter ..........................................................................2-21

2.11 Attaching epi-fluorescence illuminator ............................................................2-22

2.11.1 Filter stop carrier for epi-fluorescence illuminator ......................................................... 2-23

2.11.2 Attaching HBO 50 fluorescent lamp .............................................................................. 2-24

2.11.3 Replacing burner in fluorescent lamp ............................................................................ 2-25

2.11.4 Adjusting burner in fluorescent lamp ............................................................................ 2-27

2.11.5 Changing P&C reflector module.................................................................................... 2-28

2.11.6 Changing filter set in P&C reflector module .................................................................. 2-30

2.11.7 Changing color splitter in P&C reflector module............................................................ 2-31

2.12 Connecting the instrument to the line ...........................................................2-33

3 OPERATION....................................................................................................3-3

3.1 Switching on the instrument ............................................................................3-3

3.2 Using the transmitted-light brightfield technique ..............................................3-4

3.2.1 General principle............................................................................................................. 3-4

3.2.2 Transmitted-light brightfield configuration...................................................................... 3-4

3.2.3 Setting transmitted-light brightfield for KÖHLER illumination .......................................... 3-4

3.3 Using transmitted-light phase contrast..............................................................3-6

3.3.1 General principle............................................................................................................. 3-6

3.3.2 Transmitted-light phase contrast configuration ............................................................... 3-6

3.3.3 Setting transmitted-light phase contrast.......................................................................... 3-7

3.4 Using the transmitted-light darkfield technique.................................................3-8

3.4.1 General principle............................................................................................................. 3-8

3.4.2 Transmitted-light darkfield configuration ........................................................................ 3-8

3.4.3 Setting transmitted-light darkfield................................................................................... 3-9

3.5 Using transmitted-light polarization contrast...................................................3-12

3.5.1 General principle........................................................................................................... 3-12

3.5.2 Transmitted-light polarization contrast configuration .................................................... 3-12

3.5.3 Setting transmitted-light polarization contrast............................................................... 3-12

3.5.4 Sample differentiation between gout and pseudogout.................................................. 3-13

3.6 Setting epi-fluorescence .................................................................................3-14

3.6.1 General principle........................................................................................................... 3-14

3.6.2 Epi-fluorescence configuration ...................................................................................... 3-14

3.6.3 Setting epi-fluorescence................................................................................................ 3-14

3.7 Measurement of lengths ................................................................................3-16

3.8 Attaching ICS double tube .............................................................................3-17

IV B 40-815 e 12/01

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Axiostar plus Contents Carl Zeiss

3.9 Attaching photomicrography equipment ........................................................3-18

3.9.1 Attaching SLR camera, e.g. CONTAX ARIA ...................................................................3-18

3.9.2 Attaching MC 80 DX microscope camera (film cassette 35 mm) ....................................3-20

3.10 Attaching adapters for video cameras.............................................................3-21

3.11 Attaching adapters for digital compact cameras..............................................3-23

3.11.1 Digital cameras with 37 mm filter thread, e.g. SONY DCR-PC100 / SONY DSC – S50.....3-24

3.11.2 Digital cameras with 52 mm filter thread, e.g. SONY DSC–S70, DSC-S75, DSC-S85......3-25

3.11.3 Attaching cameras with other filter thread sizes ............................................................3-26

3.11.4 Sony DSC-S70, DC-S75, DSC-S85 on digital camera adapter 44

M52x0.75, 000000-1108-984 .......................................................................................3-26

3.12 Axiostar plus - Packing the mount ..................................................................3-27

4 CARE, MAINTENANCE AND TROUBLE-SHOOTING .......................................4-2

4.1 Care and maintenance of the instrument..........................................................4-2

4.2 Troubleshooting...............................................................................................4-3

4.2.1 Changing the fuses..........................................................................................................4-3

4.2.2 Changing the 6 V, 20 W halogen lamp..........................................................................4-4

4.2.3 Troubleshooting ..............................................................................................................4-5

4.2.4 Spares, wearing parts and tools.......................................................................................4-8

4.3 Requesting service............................................................................................4-9

APPENDIX

Index .............................................................................................................................. A-2

List of Abbreviations .......................................................................................................A-4

Physical and Technical Units............................................................................................ A-5

Certification in Accordance with DIN EN ISO 9001 / DIN EN 46001
EC Conformity Declaration

B 40-815 e 12/01 V

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Safety

Carl Zeiss General Notes, Instrument Safety and EMC Axiostar plus

General Notes on Safety

Before starting up the microscope, please
ensure that you have familiarized yourself
thoroughly with the contents of this manual.

Further information can be obtained from our
service department or from authorized
representatives.

The Axiostar plus microscope is an optical
precision instrument which may be impaired in
its performance or damaged if handled
improperly.

The microscope must be operated by trained
personnel only who must be aware of the
possible problems involved with microscopy and
who are trained in the relevant application.

CAUTION

Hot surface; there is a risk of burns.

CAUTION!

If the safety notes are not observed, there is a
risk that the instrument will be destroyed.

☞

Notes that must be observed when working
with the microscope.

NOTE!

!

To ensure that the working procedure is safe
and that the microscope functions without
problems, the notes and warnings included in
the operating instructions must be observed.

These are highlighted in the text by the
following symbols:

CAUTION!

If the safety notes are not observed there is a
risk for the user.

CAUTION!

Disconnect the instrument from the line.

Instrument Safety and EMC

The Axiostar plus microscope was designed,
produced and tested in compliance with DIN EN
61010-1 (IEC 1010-1) “Safety requirements for

electrical measuring, control and laboratory
instruments“. It left the factory in a perfectly

safe condition.
The Axiostar plus microscope meets the
requirements of the EC directives 73/23/EC­appendix 1 and 89/336/EC and the EMC
legislation of 09/18/1998:

Radio interference suppression in accordance
with EN 55011 class B

Resistance to jamming in accordance with EN
50082-2

Conformity with the above EC directives is
documented by the CE symbol.

VI B 40-815 e 12/01

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Safety

Axiostar plus Unpacking Transport, Storage, Operation Carl Zeiss

Unpacking, Transport, Storage

Please observe the following safety notes when
unpacking, transporting and storing the
Axiostar plus microscope:

– In accordance with standard practice, the

microscope is supplied in a plastic container
with cardboard packaging; only use the
original packaging for transport.

– Retain the packaging for longer storage

periods or to return the instrument to the
manufacturer.

– When unpacking, use the delivery note to

ensure that all configurations and modules
are present.

– Observe transport and storage tempera-

tures in accordance with the technical data.

– Place the microscope on a stable work

surface.

Operation

Please observe the following safety notes when
using the Axiostar plus microscope:

– The microscope can be operated with a line

voltage of 100 − 240 V.

– The microscope must be connected only to

a properly installed outlet featuring a
grounding contact; the grounding effect
should not be made ineffective with an
extension cable that does not have a
protective ground wire.

– If it is established that the protection meas-

ures are no longer effective:

⇒

switch off the microscope

⇒

safeguard against inadvertent operation

⇒

contact an authorized workshop or the

manufacturer for repairs.

– Keep optical surfaces free of fingerprints.

Disposal

When disposing of the Axiostar plus
microscope, please observe the following safety
notes:

– Defective microscopes should not be placed

in ordinary domestic waste;
they should be disposed of in accordance
with the relevant statutory provisions.

– Always disconnect the instrument from the

line before opening the instrument,
changing the lamps or changing the fuses!

– Only use fuses for the required rated

power!

– The use of makeshift fuses and the short-

circuiting of the fuse holders are not
permitted!

Be careful of light-emitting diode radiation
when using the LED illuminator. Do not look
directly into the illuminator with your bare eyes
from a short distance. When adjustments are
required, the intensity must be reduced
accordingly via the potentiometer, or a suitable
attenuator must be used.

B 40-815 e 12/01 VII

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Safety

Carl Zeiss Care and Maintenance, Warranty Notes Axiostar plus

Care and Maintenance

Please observe the following safety notes when
caring for and maintaining the Axiostar plus
microscope:

– With the exception of the work specified in

Chapter 4 of this manual “Care,
Maintenance and Troubleshooting“, no

maintenance or repair may be performed by
the user. We expressly indicate that any
other repairs must only be carried out by
our authorized personnel.

Damaged instruments or components must

–

only be repaired and maintained by our
service department.

– Protect the microscope against dirt, dust

and moisture; these influences can impair
the performance of the instrument.

– If the microscope is not in use, protect it

with the dust cover and make sure that it
has been switched off.

– Lamps are to be changed in accordance

with the steps in Chapter 4 “Care,
Maintenance and Troubleshooting“.

Notes on Warranty

The Axiostar plus microscope, including its
original accessories, may only be used for the
microscope techniques described in this
manual. The manufacturer cannot assume any
liability for any other applications.

Please observe the following warranty notes for
the Axiostar plus microscope:

– The manufacturer guarantees that the

instrument has no material and production
defects when delivered.

– You must inform us of any defects

immediately and do everything possible to
minimize the damage.

– If the manufacturer is informed of such a

defect, he is obligated to remove it; it is his
decision whether he does this by repairing
the instrument or by delivering an
instrument free of any defect.

– No guarantee is provided for defects caused

by natural wear (wearing parts in particular)
or improper use.

– The manufacturer is not liable for damage

caused by faulty operation, negligence or
any other tampering with the instrument, in
particular as a result of removing and
exchanging microscope components or the
use of accessories from other
manufacturers.

– Disconnect the instrument from the line

before changing a lamp.

– Let the lamps cool down before changing

– Unauthorized intervention invalidates all

claims against the warranty.

them.

– Keep the lamps free of fingerprints.

VIII B 40-815 e 12/01

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Instrument description

Axiostar plus Contents, List of Illustrations Carl Zeiss

INSTRUMENT DESCRIPTION

Contents

1 INSTRUMENT DESCRIPTION..................................................................................... 1-3

1.1 Name and intended application .................................................................................. 1-3

1.2 Description and main features .................................................................................... 1-3

1.3 System overview......................................................................................................... 1-4

1.4 Objectives .................................................................................................................. 1-7

1.5 Eyepieces ................................................................................................................... 1-9

1.6 Stage micrometers and eyepiece reticles ..................................................................... 1-9

1.7 Technical data.......................................................................................................... 1-11

List of illustrations

Fig. 1-1 Overall view ............................................................................................................... 1-2

40-815 e 12/01 1-1

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Instrument description

Carl Zeiss Overall view Axiostar plus

Fig. 1-1 Overall view

1-2 B 40-815 e 12/01

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Instrument description

Axiostar plus Intended application, Main features Carl Zeiss

1 INSTRUMENT DESCRIPTION

1.1 Name and intended application

Manufacturer‘s name:
Axiostar plus microscope

The Axiostar plus is a transmitted light
microscope for the visualization of fine
structures and forms in biology and medicine.
Typical applications of the Axiostar plus include:

– Histology, pathology,
– Doctors’ offices,
– Training (schools and universities),
– Routine laboratory microscopy,
– Immunofluorescence and FISH methods,
– Field use with LED illumination.

Key to Fig. 1-1

1 Eyepieces
2 Binocular tube
3 Microscope stand
4 Knurled screw for tube locking
5 Brightness control
6 On/off switch with integrated signal lamp
7 Fine focusing drive (two-way)
8 Coarse focusing drive (two-way)
9 Drive for adjusting mechanical stage

in X direction
10 Drive for adjusting mechanical stage in Y direction
11 Luminous field diaphragm
12 Condenser carrier
13 Lever for adjusting aperture diaphragm
14 Condenser
15 Centering screw for condenser (two-way)
16 Mechanical stage with specimen holder
17 Objective
18 4- or 5-position nosepiece

1.2 Description and main features

Thanks to its pyramid design, the Axiostar plus
is a compact transmitted-light microscope.

In addition to high-resolution ICS objectives and
the major brightfield, darkfield, phase contrast,
polarization contrast and epi-fluorescence
techniques, an optional camera port for photo
and video documentation is also available to
the user.

Major instrument features:

– Sturdy and convenient stand in the pyramid

design

– Ergonomic base plate with hand rests and

adjustable viewing height and angle

– User-friendly coaxial coarse and fine drive
– Mechanical stage 75 × 30 R/L with ceramic-

coated stage surface and specimen holder;
selectable stage drive right or left

– Space-saving and continuously adjustable

integrated illuminator with long-life 6 V,
20 W halogen lamp

– 0.9/1.25 condenser for brightfield, darkfield

and phase contrast Ph 1, 2, 3

– Ball-bearing, 4- or 5-position objective

revolver with W 0.8 thread

– ICS objectives in the price/performance

categories CP-Achromat, A-Plan and
Achroplan in finely graduated rows

– Binocular tube 45°/20 ICS with a viewing

height of 425 − 470 mm and binocular
phototube 45°/20 ICS with folding prism
100% vis / 100% doc

– Binocular tube 30°/20 ICS with a viewing

height of 430 – 475 mm and binocular
phototube 30°/20 ICS with folding prism
100 % vis / 100 % doc

– 10× eyepieces for the field numbers 20 or

18, suitable for eyeglass wearers, fixed and
adjustable

– Visual white-balancing with the white-

balance filter

40-815 e 12/01 1-3

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Instrument description

Carl Zeiss System overview Axiostar plus

1.3 System overview

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Instrument description

Axiostar plus System overview Carl Zeiss

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Instrument description

Carl Zeiss System overview Axiostar plus

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Instrument description

Axiostar plus Objectives Carl Zeiss

1.4 Objectives

The objectives are the optical centerpiece of
the microscope.
The following is an example of how objectives
can be labeled:

CP-ACHROMAT 10×/0.25 ∞/-.

where:
10× = objective magnification, with a defined

color ring on the objective
allocated to each magnification step
(Carl Zeiss color code)

0.25 = numerical aperture

∞ = infinite tube length

− = can be used with cover slip thickness

D = 0 or 0.17 mm

or

0.17 = can be used with cover slip thickness

D = 0.17 mm

and
Oil = oil immersion objective

Ph 2 = phase contrast objective with green

objective labeling and annular
diaphragm Ph 2

The objective magnification multiplied by the
eyepiece magnification (usually 10×) results in
the visual overall magnification:
e. g. 10 × 10 = 100×.

☞

The objective labeling "∞" indicates that these
objectives may only be used with microscopes
featuring infinite optics (ICS) and not with
instruments whose objectives are marked with
"160" as their mechanical tube length (in
mm).

The exact observance of the cover slip thickness
of 0.17 mm is all the more necessary the
higher the numerical aperture of the
objective. Therefore, so-called "Corr"
objectives can be set for different cover slip
thickness via a correction ring. For this, a
specimen area is searched, and the position of
the correction ring where optimum focus and
image contrast are obtained is determined
(refocusing is always required).

Immersion objectives are always insensitive to
differences in cover slip thickness.

When immersion objectives are used, the air
between the cover slip and the objective is
replaced with a liquid, which is immersion oil
in most cases. The plastic oiler containing
20 ml of 581 N Immersol is particularly
suitable for this purpose.

Due to their short working distance, objectives
25× and higher feature resilient mounts
(specimen protection).

The numerical aperture × 1000,
e. g. 0.25 × 1000 = 250×, is the highest
useful magnification, i.e. no further details are
resolved above that limit.

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Instrument Description

Carl Zeiss Objectives Axiostar plus

The following objectives are available for the Axiostar plus microscope:

Microscopy

Technique

Transm.-light brightfield CP-Achromat 5×/0.12 11.2 - 440920-0000-000

Phase contrast CP-Achromat 10×/0.25 Ph 1 5.1 - 440931-0000-000

Transm.-light brightfield A-Plan 5×/0.12 9.9 - 441020-0000-000

Phase contrast A-Plan 10×/0.25 Ph 1 4.4 - 441031-0000-000

Objective

CP-Achromat 10×/0.25 5.1 - 440930-0000-000

CP-Achromat 40×/0.65 0.3 0.17 440950-0000-000

CP-Achromat 100×/1.25 Oil 0.07 0.17 440980-0000-000

CP-Achromat 40×/065 Ph 2 0.3 0.17 440951-0000-000

CP-Achromat 100×/1.25 Oil Ph 2 0.07 0.17 000000-1007-159

CP-Achromat 100×/1.25 Oil Ph 3 0.07 0.17 440981-0000-000

A-Plan 10×/0.25 4.4 - 441030-0000-000

A-Plan 20×/0.45 0.53 0.17 441040-0000-000

A-Plan 40×/0.65 0.43 0.17 441050-0000-000

A-Plan 100×/1.25 Oil 0.22 0.17 441080-0000-000

A-Plan 20×/0.45 Ph 2 0.53 0.17 441041-0000-000

Magnification/

Numerical

aperture

Free working

distance

FAA [mm]

Cover slip

thickness

D [mm]

Cat.No.

A-Plan 40×/0.65 Ph 2 0.43 0.17 441051-0000-000

A-Plan 100×/1.25 Oil Ph 3 0.22 0.17 441081-0000-000

Transm.-light brightfield Achroplan 4×/0.10 11.1 - 440020-0000-000

Achroplan 10×/0.25 4.8 - 440030-0000-000

Achroplan 20×/0.45 2.07 0.17 440040-0000-000

Achroplan 40×/0.65 0.59 0.17 440050-0000-000

Achroplan 50×/0.90 Oil 0.29 0.17 440057-0000-000

Achroplan 63×/0.80 0.29 0.17 440060-0000-000

Achroplan 63×/0.95 0.15

Achroplan 100×/1.25 Oil 0.19 0.17 440080-0000-000

Achroplan 100×/1.25 Oil Iris 0.19 0.17 440086-0000-000

Phase contrast Achroplan 10×/0.25 Ph 1 4.8 - 440031-0000-000

Achroplan 20×/0.45 Ph 2 2.07 0.17 440041-0000-000

Achroplan 40×/0.65 Ph 2 0.59 0.17 440051-0000-000

Achroplan 100×/1.25 Oil Ph 3 0.19 0.17 440081-0000-000

Transm.-light brightfield Plan-Neofluar 2.5×/0.075 9.3 - 440310-0000-000

0

(no cover slip)

440068-0000-000

1-8 B 40-815 e 12/01

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Instrument description

Axiostar plus Eyepieces, Stage micrometers, Eyepiece reticles Carl Zeiss

1.5 Eyepieces

The following eyepieces are offered for the Axiostar plus:

Eyepiece Image angle Cat. No.

Eyepiece PL 10×/18 Br.

Eyepiece PL 10×/18 Br. foc.

Eyepiece E-PL 10×/20 Br.

Eyepiece E-PL 10×/20 Br. foc.

Eyepiece PL 16×/16 Br.

Eyepiece PL 16×/16 Br. foc.

39° 444131-9901-000

39° 444132-9901-000

43° 444231-9901-000

43° 444232-9902-000

54° 444053-0000-000

54° 444054-0000-000

We recommend using eyecups for the eyepieces; they can be ordered in the Zeiss catalog under Cat.
No. 444801-0000-000.

1.6 Stage micrometers and eyepiece reticles

Measuring and counting using the microscope requires stage micrometers and eyepiece reticles, a small
selection of which is listed below:

Illustration Description, Technical Data Cat. No.

Stage micrometer, positive 5 + 100/100 y

D = 0.17 mm

12345

mm

0

gradation on the +y-axis: 5 mm in 5 intervals;
gradation on the −y-axis:
1 mm in 100 intervals with two opposing scales =
10 µm
Accuracy ±1 µm

474026-0000-000

Crossline micrometer disk 14:140 / d = 26 mm

454060-0000-000
gradation length = 14 mm
increments = 0.1 mm

0 1 2 3 4 5 6 7 8 9 1011 1213 14

0 1 2 3 4 5 6 7 8 9 1011 1213 14

gradation tolerance ≤ 0.001 mm

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Instrument description

Carl Zeiss Eyepieces, Stage micrometers, Eyepiece reticles Axiostar plus

Crossline disk / d = 26 mm 474064-0000-000

0
1
2
3
4
5
6
7
8
9

10

Crossline micrometer disk 10:100 / d =
26 mm

gradation length = 10 mm
increments = 0.1 mm
gradation tolerance ≤ 0.001 mm

Net micrometer

474066-9901-000

474068-0000-000

12.5××××12.5/5;10 / d = 26 mm

area 12.5 ×××× 12.5 mm, divided in fields of 5 ×××× 5 or
10 ×××× 10

Photo reticle MC 2.5×××× / d = 26 mm

454075-0000-000
for 35mm photography with an additional
magnification of 2.5×××× or for large-format
photography with a 10×××× additional magnification

Fixed pointer for focusing eyepiece /

000000-1095-418

d = 26 mm

To point toward objects in the specimen

☞

If an eyepiece reticle is used, the binocular tube or the phototube must be equipped with two

foc. eyepieces containing an adjustable eye lens, into one of which the eyepiece reticle is
mounted.

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Instrument description

Axiostar plus Technical Data Carl Zeiss

1.7 Technical data

(1) Dimensions (width ×××× depth ×××× height)

Stand with binocular tube......................................................................approx. 200 × 350 × 450 mm

Stand with phototube............................................................................approx. 200 × 350 × 450 mm

Overall height including T2 adapter and CONTAX ARIA SLR camera housing.............. approx. 570 mm

(2) Weight

Axiostar plus with binocular tube ..................................................................................approx. 6.7 kg

(3) Ambient conditions

Storage and transport (in packaging):

Permissible ambient temperature ...................................................................................-40 to +70 °C

Permissible relative humidity (without condensation) ........................................................max. 100 %

Operation:

Permissible ambient temperature ..................................................................................+10 to +35 °C

Permissible relative humidity (without condensation) ..........................................................max. 85 %

Air pressure........................................................................................................800 hPa to 1060 hPa

(4) Operating data

Category of use..............................................................................................................closed rooms

Protection class..................................................................................................................................I

Protection type........................................................................................................................... IP 20

Electrical safety.......................................... in compliance with DIN EN 61010-1 (IEC 1010-1) including

.........................................................................................................................CSA and UL directives

Pollution degree ...............................................................................................................................2

Excess voltage category .................................................................................................................... II

Radio interference suppression....................................................in accordance with EN 55011 Class B

Line voltage...................................................................................................... 100 to 240 V (±10 %)

Conversion of the voltage is not necessary due to the wide range power supply!

Line frequency..................................................................................................................50 to 60 Hz

Power consumption..........................................................................................................max. 65 VA

Output voltage ........................................................................... stabilized, adjustable from 1.5 to 6 V

(5) Fuses in accordance with IEC 127

for 100 − 240 V.........................................................................................T 0.8 A; 250 V; 5 × 20 mm

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Instrument description

Carl Zeiss Technical Data Axiostar plus

(6) Light sources

Halogen lamp.............................................................................................................. HAL 6 V, 20 W

Adjustment of the light source ........................................................ continuous, 1.5 to 6 V DC

Color temperature at 6 V ...........................................................................................2800 K

Light flux ..................................................................................................................... 280 lm

Average life .................................................................................................................1000 h

Luminous area ...................................................................................................2.0 × 2.0 mm

LED Illumination

Constant, brightness independent color temperature of................................................7480 K

Homogenous image field illumination ................................................... up to 20 mm diameter

Suitable for objectives with magnification of .......................................................2.5 x to 100 x

Analog brightness control of ...................................................................approx. 15 to 100 %

Operation with 9 V alkaline battery / 9 V NiCd Accublock:

Operating voltage.......................................................................... approx. 4.5 to 10 V

Current at maximum brightness depending on operating voltage of:

Operating voltage 4.5 V.................................................................................... 90 mA

Operating voltage 9 V....................................................................................... 51 mA

Operating voltage 10 V..................................................................................... 45 mA

Average life with 9 V alkaline battery (depending on battery capacity):

Maximum intensity................................................................................. approx. 6.5 h

65 % of maximum intensity..................................................................... approx. 16 h

50 % of maximum intensity..................................................................... approx. 21 h

Operation with 12 V battery / Accu (voltage between 9-13.5 V):

Operating voltage 9 V....................................................................................... 88 mA

Operating voltage 12 V..................................................................................... 60 mA

Operating voltage 13.5 V.................................................................................. 50 mA

Fluorescent Lamp HBO 50

Lamp voltage.............................................................................. L1: 39 45 V / L2: 34 to 39 V

Lamp current ....................................................................................... L1: 1.30 A / L2: 1.45 A

Power ........................................................................................................................... 50 W

Average light current .................................................................................................2000 lm

Average life ...................................................................................................................100 h

Illumination surface................................................................................................ 0.3x1 mm

Power supply mbq52ac-z for HBO 50

Line voltage ................... 100,110,120,127,230, 240 V, ±10 % AC, can be switched externally

Line frequency ............................................................ 50 to 60 Hz, can be switched externally

Power consumption. ...........................................................................................max. 350 VA

1

2

1

For photography using artificial light color reversal film for 3200 K, the conversion filter CB 3 (467852)
produces the correct color temperature in the light path.

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Instrument description

Axiostar plus Technical Data Carl Zeiss

(7) Opto-mechanical data

2

Stand with stage focusing........................................................................with coarse drive

(4 mm/U)

and fine drive (0.4 mm/U)

overall lift 15 mm

Objective change............................................................... manually via 4/5-position objective revolver

Objectives ..............................................................................ICS line of objectives with W 0.8 thread

Eyepieces ......................................................................................................30 mm plug-in diameter

with field number 18.................................................. PL 10×/18 Br. and PL 10×/18 Br. foc. or

with field number 20................................................E- PL 10×/20 Br. and E- PL 10×/20 Br. foc.

Specimen stage ..................................................... mechanical stage 75 × 30 R/L with ceramic surface

Dimensions (width × depth).............................................................................. 170 × 140 mm

Travel range (width × depth) ................................................................................ 75 × 30 mm

Coaxial drive ..........................................................................................right or left selectable

Vernier............................................................................................... readable from the right

Specimen holder..............................................................................with spring clip to the left

Abbe-condenser 0.9/1.25 .............................for V

< 4× with wide angle function 445312-0000-000

Obj.

Binocular tube 45°/20

maximum field number .......................................................................................................20

interpupillary distance ........................................................can be set between 55 and 75 mm

viewing angle.................................................................................................................... 45°

viewing height ................................................................................................. 425 − 470 mm

visual port.........................................................................................................tube factor 1×

Binocular phototube 45°/20

maximum field number .......................................................................................................20

interpupillary distance ........................................................can be set between 55 and 75 mm

viewing angle.................................................................................................................... 45°

viewing height ................................................................................................. 425 − 470 mm

visual port.........................................................................................................tube factor 1×

camera/video port ............................................................................................ tube factor 1×

camera/video port..........................................................................................interface 60 mm

switched via folding prism......................................................................100% vis / 100% doc

2

The scale on the coarse drive (0 to 400) permits the orienting measurement of the object thickness:
1 increment corresponds to approx. 5 µm

40-815 e 12/01 1-13

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Instrument description

Carl Zeiss Technical Data Axiostar plus

Binocular tube 30°/20

maximum field number .......................................................................................................20

interpupillary distance.........................................................can be set between 55 and 75 mm

viewing angle.................................................................................................................... 30°

viewing angle with ergonomic base plate............30°, can be adjusted by ±6.5° in steps of 2.2°

viewing height ................................................................................................430 to 475 mm

viewing height with ergonomic base plate (4 settings each):

binocular component turned down...................................... 480 / 490 / 500 / 510 mm

binocular component turned up........................................... 525 / 535 / 545 / 555 mm

visual port......................................................................................................... tube factor 1x

Binocular phototube 30°/20

maximum field number .......................................................................................................20

interpupillary distance.........................................................can be set between 55 and 75 mm

viewing angle.................................................................................................................... 30°

viewing angle with ergonomic base plate.................... 30°, adjustable by ±6.5° in steps of 2.2°

viewing height ...........................................................................................................430 mm

viewing height with ergonomic base plate (4 settings each):

binocular component turned down...................................... 480 / 490 / 500 / 510 mm

binocular component turned up........................................... 525 / 535 / 545 / 555 mm

visual port.........................................................................................................tube factor 1×

camera/video port ............................................................................................ tube factor 1×

camera/video port ..........................................................................................interface 60 mm

switched via folding prism ......................................................................100% vis / 100% doc

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Start-Up

Axiostar plus Contents Carl Zeiss

START-UP

Contents

2 START-UP ..................................................................................................................2-3

2.1 Unpacking the instrument ...........................................................................................2-3

2.2 Attaching and setting ergonomic base plate ................................................................2-3

2.2.1 Attaching the ergonomic base plate ............................................................................2-4

2.2.2 Adjusting the ergonomic base plate.............................................................................2-6

2.3 Attaching tubes...........................................................................................................2-8

2.3.1 Attaching binocular tube 45°/20 ICS ............................................................................2-8

2.3.2 Attaching binocular phototube 45°/20 ICS...................................................................2-8

2.3.3 Attaching binocular tube 30°/20 ICS............................................................................2-9

2.4 Screwing in objectives ...............................................................................................2-10

2.5 Inserting eyepieces ....................................................................................................2-10

2.5.1 Inserting eyepiece reticles ..........................................................................................2-11

2.5.2 Compensation of ametropia when eyepiece reticles are used ....................................2-11

2.5.3 Attaching folding eyecups .........................................................................................2-12

2.6 Setting interpupillary distance and viewing height ......................................................2-12

2.7 Setting mechanical stage 75 x 30 R/L .........................................................................2-13

2.7.1 Setting drive length on the stage drive.......................................................................2-13

2.7.2 Setting drive knob torque for x/y adjustment on the mechanical stage........................2-13

2.8 Attaching condensers................................................................................................2-14

2.8.1 Attaching Abbe condenser 0.9/1.25 ..........................................................................2-14

2.8.2 Inserting slider for phase contrast or darkfield ...........................................................2-14

2.8.3 Attaching Abbe condenser with 5-position turret.......................................................2-15

2.8.4 Attaching LED illuminator ..........................................................................................2-16

2.8.5 Attaching darkfield condenser ...................................................................................2-18

2.9 Attaching colored disk carrier, polarizer or wide-angle unit.........................................2-20

2.10 Inserting white-balance filter......................................................................................2-21

2.11 Attaching epi-fluorescence illuminator .......................................................................2-22

2.11.1 Filter stop carrier for epi-fluorescence illuminator ......................................................2-23

2.11.2 Attaching HBO 50 fluorescent lamp...........................................................................2-24

2.11.3 Replacing burner in fluorescent lamp.........................................................................2-25

2.11.4 Adjusting burner in fluorescent lamp .........................................................................2-27

2.11.5 Changing P&C reflector module ................................................................................2-28

2.11.6 Changing filter set in P&C reflector module ...............................................................2-30

2.11.7 Changing color splitter in P&C reflector module.........................................................2-31

2.12 Connecting the instrument to the line.......................................................................2-33

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Start-Up

Carl Zeiss List of illustrations Axiostar plus

List of illustrations

Fig. 2-1 Inserting base pods......................................................................................................2-4

Fig. 2-2 Attaching ergonomic base plate ..................................................................................2-5

Fig. 2-3 Adjusting ergonomic base plate...................................................................................2-7

Fig. 2-4 Attaching binocular tube / phototube 45°....................................................................2-8

Fig. 2-5 Attaching binocular tube / phototube 30°....................................................................2-9

Fig. 2-6 Screwing in objectives ...............................................................................................2-10

Fig. 2-7 Inserting eyepieces ....................................................................................................2-10

Fig. 2-8 Inserting eyepiece reticle............................................................................................2-11

Fig. 2-9 Setting interpupillary distance ....................................................................................2-12

Fig. 2-10 Setting viewing height...............................................................................................2-12

Fig. 2-11 Setting torque ...........................................................................................................2-13

Fig. 2-12 Attaching Abbe condenser 0.9/1.25...........................................................................2-14

Fig. 2-13 Inserting slider for phase contrast or darkfield ...........................................................2-14

Fig. 2-14 Attaching Abbe condenser with 5-position turret .......................................................2-15

Fig. 2-15 Attaching LED illuminator ..........................................................................................2-16

Fig. 2-16 Connecting LED illuminator to power source..............................................................2-17

Fig. 2-17 Attaching darkfield condenser ...................................................................................2-18

Fig. 2-18 Attaching colored disk carrier, polarizer or wide angle unit........................................2-20

Fig. 2-19 Inserting white-balance filter......................................................................................2-21

Fig. 2-20 Attaching epi-fluorescence illuminator .......................................................................2-22

Fig. 2-21 Filter stop carrier .......................................................................................................2-23

Fig. 2-22 Attaching HBO 50 .....................................................................................................2-24

Fig. 2-23 Replacing burner .......................................................................................................2-25

Fig. 2-24 Adjusting burner .......................................................................................................2-27

Fig. 2-25 Removing/inserting reflector carriage .........................................................................2-28

Fig. 2-26 Removing/inserting P&C reflector module ..................................................................2-29

Fig. 2-27 Changing filter set in P&C reflector module................................................................2-30

Fig. 2-28 Inserting filter and color splitter .................................................................................2-31

Fig. 2-29 Changing color splitter ..............................................................................................2-31

Fig. 2-30 Changing color splitter ..............................................................................................2-32

Fig. 2-31 Color splitter designation...........................................................................................2-32

Fig. 2-32 Connecting the instrument to the line........................................................................2-33

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Start-Up

Axiostar plus Attaching and adjusting ergonomic base plate Carl Zeiss

2 START-UP

☞

Before installing and starting-up the Axiostar
plus, carefully read through and observe the
notes on safety (page VI ff.).

To prevent fingerprints, do not touch optical
surfaces when unpacking!

2.1 Unpacking the instrument

The Axiostar plus microscope, including
accessories, is delivered in standard packaging.

• Remove the microscope from the transport

case and place it on the work table.

• Keep the packaging so that the instrument

can be stored for a longer period of time or
returned to the manufacturer.

2.2 Attaching and setting

ergonomic base plate

Using the Axiostar plus with ergonomic base
plate allows you to set the microscope to your
individual ergonomic requirements.

An experienced technician can attach the
Axiostar plus (000000-1169-149, -150, -151) or
Axiostar (000000-1122-098, -099, -100) to the
ergonomic base plate on his own. However, we
recommend asking another person to help.

Before attaching the ergonomic base plate,
unplug the microscope.

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Start-Up

Carl Zeiss Unpacking, Attaching ergonomic base plate Axiostar plus

2.2.1 Attaching the ergonomic base plate

• Remove the tube if one is attached.

• Tilt the stand back and set it down.

• Remove the four plastic feet (Fig. 2-1/1)

from the stand and replace with the four
base pods included (Fig. 2-1/2). If necessary,
clean adhesive or varnish remains from the
drilled holes.

• Turn over the stand and place it top side

down on a soft surface or the packing
cardboard (Fig. 2-2).

• Secure the microscope in this position or

have a second person hold it to avoid
damaging the microscope.

• Place the ergonomic base plate (Fig. 2-2/1)

on the bottom of the stand and fasten with
the four Allen screws (SW 2.5 M4 x 30

1 Plastic feet
2 Base pod

Fig. 2-1 Inserting base pods

(Fig. 2-2/2) using the four washers.

• Turn the microscope over and set it down

on the ergonomic base plate.

• Reattach tube and secure.

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Start-Up

Axiostar plus Attaching and adjusting ergonomic base plate Carl Zeiss

1 Ergonomic base plate
2 Allen screws M4 x 30

Fig. 2-2 Attaching ergonomic base plate

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Start-Up

Carl Zeiss Attaching and adjusting ergonomic base plate Axiostar plus

2.2.2 Adjusting the ergonomic base plate

Adjusting viewing height

The viewing height of the microscope can be
adjusted by turning the feet (2-3/3) on the base
plate in four levels. The height difference is 10
mm per level (total lift 30 mm).

When the viewing height is changed, the slope
of the hand rest on the ergonomic base plate is
changed automatically. The adjustment range is
approximately 6.5°.

When setting the viewing height, there is a risk
of getting your hands caught between the table
and ergonomic base plate or adjustment feet;
therefore, do not place your hands under the
ergonomic base plate or adjustment feet when
setting it down.

Setting viewing angle (microscope slope)

The slope of the microscope in relation to the
table surface or hand rest can be set according
to your needs and application. There are four
settings (adjustment range 6.5° in steps of

2.2°).

In one setting, the microscope is basically
parallel to the table surface.

• Grasp the microscope at the top of the

stand (2-3/1)and lift it by tilting it back
slightly; while doing so, press the locking
button (2-3/4) until it unlocks.

• Tilt the microscope backward or forward

until you reach the desired angle.

• Let go the the locking button when you

reach the desired position. When the
button engages, let go of the stand.

• To set the height, lift the entire unit with

the handle on the back of the ergonomic
base plate (2-3/2). The adjustment feet
(2-3/3) will disengage and can be adjusted.

• Select desired height by turning the

adjustment feet, then lower the entire unit.
The adjustment feet will engage in position
automatically.

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Start-Up

Axiostar plus Attaching and adjusting ergonomic base plate Carl Zeiss

1 Top of stand
2 Handle on back of ergonomic base plate
3 Adjustment feet
4 Locking button

Fig. 2-3 Adjusting ergonomic base plate

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Start-up

Carl Zeiss Attaching tube Axiostar plus

2.3 Attaching tubes

One knurled screw and one hexagonal screw
each are supplied with the mount. Any of these
screws can be used to clamp the tube
(phototube) to the stand.

• Screw the required screw into the threaded

hole (2-4/6) in the stand.

2.3.1 Attaching binocular tube 45°/20 ICS

• Remove dust cover (2-4/1) from tube

underside. Unscrew the knurled screw
(2-4/5) or the hexagonal screw (2-4/4) from
the stand.

• Press the binocular tube (2-4/2) in a slightly

inclined position to the right into the spring
of the stand.

1Dust cap
2 Binocular tube
3 Binocular phototube
4 Hexagonal screw
5 Knurled screw
6 Threaded hole for knurled or hexagonal screw

Fig. 2-4 Attaching binocular tube / phototube 45°

• Then set the tube in the dovetail of the

stand by lowering the left side. The tube is
now secured from falling out.

• Turn the tube to the desired observation

position; both the illustrated rotation and
the 180° backward rotation are possible.

• Tighten the knurled screw (2-4/5) or the

hexagonal screw (2-4/4).

2.3.2 Attaching binocular phototube

45°/20

ICS

• Remove dust caps (2-4/1); loosen knurled

screw (2-4/5) or hexagonal screw (2-4/4).

• Attach the binocular phototube (2-4/3) to

the stand mount in the same way as the
binocular tube.

• Tighten knurled screw (2-4/5) or hexagonal

screw (2-4/4).

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Start-up

Axiostar plus Attaching tube Carl Zeiss

2.3.3 Attaching binocular tube 30°/20

ICS

Using the binocular tube/phototube 30° ICS
with the ergonomic base plate offers the best
ergonomic conditions for microscope use. The
adapter for 30° tubes is required to attach the
binocular tube 30°
first remove the 45°

ICS to the stand. If necessary,

ICS tube from the stand.

• Remove dust cap from tube underside.

Loosen knurled screw (2-5/4) or hexagonal
screw on the stand.

• Press the adapter for 30° tubes (2-5/2)

slightly to the right into the spring of the
stand.

• Then set the adapter in the dovetail of the

stand by lowering the left side. This secures
the adapter from falling out.

• Turn the adapter to align the outer edges

parallel to the stand; the clamping screw for
the 30° tube should point to the right.

• Tighten knurled screw (2-5/4) or hexagonal

screw.

• Place the 30°

ICS tube (2-5/1) in the adapter

(2-5/2), align the outer edges and tighten
clamping screws (2-5/3) on the adapter.

Proceed the same way when attaching the
phototube 30°

ICS.

1 Binocular tube 30° ICS
2 Adapter for 30° tubes
3 Clamping screw
4 Knurled screw

Fig. 2-5 Attaching binocular tube / phototube 30°

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Start-up

Carl Zeiss Screwing in objectives, Inserting eyepieces Axiostar plus

2.4 Screwing in objectives

• Remove dust caps (2-6/2) according to the

number of objectives and screw objectives
(2-6/3) into nosepiece (2-6/1) clockwise one
by one, starting with the lowest
magnification.

☞

Place dust caps on the unused objective
openings in the nosepiece.

1 4- or 5-position nosepiece
2Dust cap
3Objective

Fig. 2-6 Screwing in objectives

2.5 Inserting eyepieces

• Remove both protection caps (2-7/4) from

the binocular tube (2-7/3).

• Insert the fixed eyepiece, e. g. PL 10×/18 Br.

(2-7/2), into the right tube and the
focusing eyepiece PL 10×/18 Br. foc. (2-7/1)
into the left tube.

☞

The focusing eyepiece is used to compensate
for ametropia of the eyes.

1 Focusing eyepiece
2 Fixed eyepiece
3 Binocular tube
4 Dust caps

Fig. 2-7 Inserting eyepieces

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Start-Up

Axiostar plus Inserting eyepieces Carl Zeiss

2.5.1 Inserting eyepiece reticles

The eyepieces PL 10×/18 Br. foc. and
E-PL 10×/20 Br. foc. are intended for use with
eyepiece reticles (see chapter 1.6).
The slight image shift caused by the additional
path through the glass is taken into account
on the diopter scale by the fact that the zero
point position is indicated not by the white
dot (2-8/W) but by the red dot (2-8/R).
The eyepiece reticles (2-8/3) have been
attached to screw-in mounts (2-8/4) to allow
easy replacement. Complete mounts with
eyepiece reticles can be obtained directly from
Zeiss. When reordering reticles for already
available eyepieces 444232-9902-000, you
must also order the mount 444232-0206-000.
For eyepieces 444132-9901-000, order the
mount 444132-0206-000. The eyepiece
reticle will be delivered already in the mount.

To replace the mount:

• Unscrew the mount containing the

eyepiece reticle from the eyepiece.

• Screw in the new mount containing the

required eyepiece reticle.

R Zero point position of diopter scale with

eyepiece reticle

W Zero point position of diopter scale without

eyepiece reticle
1 Eyecup
2 Spectacle protection ring
3 Eyepiece reticle
4 Mount

Fig. 2-8 Inserting eyepiece reticle

2.5.2 Compensation of ametropia

when eyepiece reticles are
used

The correct use of an eyepiece reticle requires
two focusing eyepieces, e. g. PL 10×/18
Br. foc., to make it possible to compensate for
possible ametropia of the observer's eyes.

• Use the eyelens of the focusing eyepiece

to focus on the line figure of the eyepiece
reticle.

• Focus on the microscope image of a

specimen via the focusing drive by looking
through the eyepiece with reticle.

• When the image and the eyepiece reticle

are in focus in this eyepiece, focus the
image for the second eye via the focusing
eyelens of the second eyepiece.

Both microscope images and plates should be
focused.

Now focusing should occur only with the
focusing drive.

B 40-815 e 12/01 2-11

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Start-Up

Carl Zeiss Setting interpupillary distance and viewing height Axiostar plus

2.5.3 Attaching folding eyecups

The eyepieces are equipped with rubber rings
to protect eyeglasses from scratches. These
rings can be replaced with folding eyecups, if
desired.

• Remove the rubber rings (2-8/2) from the

eyepieces and attach the eyecups (2-8/1).

Sometimes, the spectacle protection rings fit
very tightly in the eyepiece groove so that a dull
object (e.g. tooth pick) is required to press
them out.

2.6 Setting interpupillary distance

and viewing height

• The eyepiece distance is matched to the

individual interpupillary distance of the
observer by swinging the eyepiece tubes
symmetrically towards one another
(Fig. 2-9).

Fig. 2-9 Setting interpupillary distance

Fig. 2-10 Setting viewing height

The interpupillary distance is correct if you see
only one round image when you look in both
eyepieces!

• The viewing height can be matched to the

requirements of the individual user by
swinging the eyepiece tubes up
(2-10/A) or down (2-10/B).

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Start-Up

Axiostar plus Setting mechanical stage 75 x 30 R/L Carl Zeiss

2.7 Setting mechanical stage 75 x 30 R/L

The Axiostar plus stand is delivered from the
factory with right or left (R/L) drive operation.

A specimen holder with spring lever is attached
on the left of the mechanical stage.

The verniers for x- and y- adjustment can be
found on the right side of the mechanical
stage.

2.7.1 Setting drive length on the

stage drive

The drive length of the x- and y-drive can be
changed within a range of approximately 15
mm by moving the drive knob (2-11/4 or 1).

2.7.2 Setting drive knob torque for

x/y adjustment on the

1 y-adjustment drive knob
2 Nut for y torque setting
3 Nut for x torque setting
4 x-adjustment drive knob
5 Adjustment pin

Fig. 2-11 Setting torque

mechanical stage

The drive knob torque is set to an average value
from the factory. To change the preset setting,
proceed as follows:

X-drive

• Move the drive knob for x-adjustment

(2-11/4) all the way down.

• Remove the adjustment pin (2-11/5) from

the y-adjustment drive knob (2-11/1) and
place into the bottom nut (2-11/3).

• Hold drive knob for x-adjustment (2-11/4)

and turn nut with adjustment pin clockwise
(lesser torque: -) or counterclockwise
(greater torque: +). See Figure 2-11.

• Do not adjust by more than one rotation.

Y-drive

• Push drive knob for y-adjustment (2-11/1)

all the way up.

• Place adjustment pin (2-11/5) in the top nut

(2-11/2).

• Hold drive knob for y-adjustment (2-11/4)

and turn nut with adjustment pin clockwise
(lesser torque: -) or counterclockwise
(greater torque: +).

• Do not adjust by more than one rotation.

• Place adjustment pin back in y-adjustment

drive knob (2-11/1).

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Carl Zeiss Attaching condensers Axiostar plus

2.8 Attaching condensers

2.8.1 Attaching Abbe condenser

0.9/1.25

• Unscrew both centering screws (2-12/3) on

the condenser carrier (2-12/1) until the
Abbe condenser 0.9/1.25 (2-12/7) can be
easily inserted into the condenser carrier.

• Insert the condenser into the condenser

carrier in such a way that

1. the dovetail of the condenser is pressed

against the spring pin (2-12/2) in the
condenser carrier and

2. the orientation screw (2-12/5) on the

underside of the condenser enters the
groove (2-12/4) on the condenser

1 Condenser carrier
2 Spring pin
3 Centering screws
4 Groove
5 Orientation screw
6 Aperture diaphragm lever
7 Condenser

• Tighten both centering screws (2-12/3) on

carrier.

the condenser carrier (2-12/1) until they
engage in the dovetail and keep the
condenser (2-12/7) in position.

Fig. 2-12 Attaching Abbe condenser 0.9/1.25

1 Condenser
2 Guiding slot
3 Slider for phase contrast or darkfield
4 Orientation screw

Fig. 2-13 Inserting slider for phase contrast

or darkfield

2.8.2 Inserting slider for phase

contrast or darkfield

• Insert the slider (2-13/3) until it engages in

the opening in the condenser (2-13/1); the
orientation screw (2-13/4) must face
upwards and engage in the guiding slot
(2-13/2).

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Axiostar plus Attaching condensers Carl Zeiss

2.8.3 Attaching Abbe condenser with

5-position turret

Attachment is made in the same way as the
Abbe condenser 0.9/1.25.

• Loosen both centering screws (2-14/3) on

the condenser carrier (2-14/1).

• Press condenser with dovetail against the

spring pin (2-14/2) in the condenser carrier.
Make sure that the orientation screw
(2-14/5) on the condenser engages in the
groove (2-14/4) of the condenser carrier.

• Tighten both centering screws (2-14/3) on

the condenser carrier (2-14/1) until they
engage in the dovetail and hold the
condenser (2-14/7) in position.

1 Condenser carrier
2 Spring pin
3 Centering screws
4 Groove
5 Orientation screw
6 Aperture diaphragm lever
7 Condenser

Fig. 2-14 Attaching Abbe condenser with 5-position

turret

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Carl Zeiss Attaching condensers Axiostar plus

2.8.4 Attaching LED illuminator

• Insert LED illuminator (2-15/3) in the

condenser carrier (2-15/1) in the same way
as the Abbe condensers.

• Tighten both centering screws (2-15/2) until

the LED illuminator lies approximately in the
center of the beam path.

• Use condenser drive (2-15/4) to move the

condenser carrier into the topmost position.

Power to the LED illuminator can be supplied
either via the line power unit or, independent
of the line, via a commercially available 9 V
Alkaline battery (9 V / Ni Cd battery block) in
the integrated battery compartment, or via an
external voltage source ranging from 11 to
14 V DC (e.g. 12 V car battery or battery

1 Condenser carrier
2 Centering screw
3 LED illuminator
4 Condenser drive

Fig. 2-15 Attaching LED illuminator

pack C). The external voltage source must
permit a constant current of up to approx.
120 mA to be loaded.

The LED illuminator provides pleasant, color­neutral white light with a constant color
temperature (approx. 7500 K) independent of
the set brightness. It is suitable for objectives
ranging from 2.5x to 100x. The light-emitting
diodes have a long life and cause only minor
operating costs.

Operation with power unit

Be careful of light-emitting diode radiation
when using the LED illuminator. Do not look
directly into the illuminator with your bare eyes
from a short distance. When adjustments are
required, the intensity must be reduced
accordingly via the potentiometer, or a suitable
attenuator must be used.

☞

The operation life with battery operation

• Insert country-specific adapter (2-16/10)

into the power unit (2-16/9).

• Insert angled connector of the power unit

(2-16/9) into the +12 V socket (2-16/2) of
the LED illuminator (2-16/1).

• Connect power unit to the line.

• Attach the cable to the back of the stand

with the self-sticking cable ties included.

strongly depends on the brightness set and the
loading capacity of the battery.

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Axiostar plus Attaching condensers Carl Zeiss

Operation with 9 V Alkaline battery
(9 V / Ni Cd battery block)

• Turn holding screw (2-16/8) on the cover

(2-16/7) of the battery compartment to the
left and remove the cover.

• Insert 9 V Alkaline battery (2-16/6) into the

battery compartment (2-16/5) with the
terminals in front (plus pole points
upwards).

• Attach cover, press it on and fix it in

position by turning the holding screw to the
right.

Operation with external voltage supply

• Insert angled connector of the 12 V battery-

adapter cable (2-16/11) into the +12 V
socket (2-16/2) of the LED illuminator.

• Connect cable clamps of the 12 V battery-

adapter cable (2-16/11) to the used 12 V
battery:

red clamp to plus pole (+),
black clamp to minus pole (-).

Depending on the brightness set via the control
(2-16/3), the integrated current control
guarantees constant brightness as long as there
is sufficient loading capacity from the battery. If
the loading capacity is exhausted, the LED
illuminator will go out or drop to minimum
brightness.

• After connection to the voltage source,

switch the LED illuminator on or off via the
toggle switch (2-16/4).

• Match the illumination intensity via the

1 LED illuminator
2 +12 V socket
3 Control
4 Toggle switch
5 Battery compartment
6 9 V Alkaline battery
7 Cover
8 Holding screw
9 Power unit
10 Country-specific adapter
11 12 V battery-adapter cable (4 m long)
12 Cable holder

control (2-16/3).

Fig. 2-16 Connecting LED illuminator to power

source

Operation with battery pack C

• Use the included connection cables to

connect a 12 V connection box on the
battery pack C (cigarette lighter) with the
12V connection box on the LED illuminator.

Make absolutely sure to avoid moistening of
the 12 V battery-adapter cable with liquids.

For further details regarding the battery pack C,
please see the applicable operating manual.

B 40-815 e 12/01 2-17

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Carl Zeiss Attaching condensers Axiostar plus

2.8.5 Attaching darkfield condenser

The darkfield condenser frame (for Axiostar
plus) is required to place darkfield condensers in
the condenser carrier.

• Place darkfield condenser (2-17/6) in the

darkfield condenser frame (2-17/5) and
screw on fastening ring (2-17/7)

☞

The dry darkfield condenser (0.7/0.85, 465506­000-000) is screwed directly into the darkfield
condenser frame without the fastening ring.

• Press the darkfield condenser frame

(2-17/5) against the spring pin (2-17/2) into
the condenser carrier (2-17/1).

1 Condensor carrier
2 Spring pin
3 Centering screws
4 Groove
5 Darkfield condenser frame
6 Darkfield condenser
7 Fastening ring

• Tighten both centering screws (2-17/3) on

the condenser carrier (2-17/1) until they
grip the ring mount and hold the darkfield
condenser frame (2-17/5) in place.

Fig. 2-17 Attaching darkfield condenser

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Axiostar plus Darkfield condenser Carl Zeiss

Selection of recommended combinations: darkfield condenser – objective for best contrast
when using transmitted-light darkfield technique

Recommended darkfield condensers:

A Ultracondenser 1.2 / 1.4 465500-0000-000 (applicable from 0.75 - 1)

B Dry darkfield condenser 0.8 / 0.95 465505-0000-000 (applicable from 0.6 – 0.75)

C Dry darkfield condenser 0.7 / 0.85 465506-0000-000 (applicable from 0.4 – 0.6)

Objective Cat. No. Magnification Aperture Darkfield condenser

A - Plan 441050-0000-000 40 X 0.65 B

A - Plan 441040-0000-000 20 X 0.45 C

Achroplan 440086-0000-000 100 X 1.25 A

Achroplan 440057-0000-000 50 X 0.90 A

Achroplan 440050-0000-000 40 X 0.65 B

Achroplan 440040-0000-000 20 X 0.45 B, C

Achroplan 440068-0000-000 63 X 0.95 A

Achroplan 440060-0000-000 63 X 0.80 A

Achrostigmat 440286-0000-000 100 X 1.25 A

CP - Achromat 440953-9901-000 50 X 0.80 A

CP - Achromat 440950-0000-000 40 X 0.65 B

CP - Achromat 440943-0000-000 20 X 0.40 C

Fluar 440257-0000-000 40 X 1.30 A

Plan - Apochromat 440786-9902-000 100 X 1.30 A

Plan - Apochromat 440786-0000-000 100 X 1.30 A

Plan - Apochromat 440756-0000-000 40 X 1.00 A

Plan - Neofluar 000000-1031-173 100 X 1.30 A

Plan - Neofluar 440364-0000-000 63 X 0.95 A

Plan - Neofluar 440456-0000-000 40 X 1.30 A

Plan - Neofluar 440486-0000-000 100 X 1.30 A

Plan - Neofluar 440544-0000-000 25 X 0.80 A

Plan - Neofluar 440350-9902-000 40 X 0.75 A, B

Plan - Neofluar 440466-0000-000 63 X 1.25 A

Plan - Neofluar 440542-0000-000 25 X 0.80 A

B 40-815 e 12/01 2-19

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Carl Zeiss Attaching colored disk carrier, polarizer or wide-angle unit Axiostar plus

2.9 Attaching colored disk carrier,

polarizer or wide-angle unit

The polarizer must be installed if you want to
use polarization contrast. The wide-angle unit
must be used with objectives having a
magnification < 4x so that the entire visible
field is illuminated.

• Hold colored disk carrier (2-18/2) parallel to

the underside of the condenser carrier
(2-18/1) and screw the holding bolts of the
colored disk carrier (2-18/6) into the front
thread on the bottom left of the condenser
carrier (2-18/1, position a) using the 90°-
offset hexagonal key SW 2 (2-18/5) until it
engages.

• Screw stop bolt (2-18/3) with adjusting lever

(2-18/4) into the rear thread on the
condenser carrier (2-18/1, position b) until it
engages.

1 Condenser carrier
2 Colored disk carrier
3 Stop bolt
4 Adjusting lever
5 Hexagonal key SW 2
6 Holding bolt
7 Slotted screwdriver
8 Wide angle unit or swiveling polarizer

Fig. 2-18 Attaching colored disk carrier, polarizer or

wide angle unit

• Screw wide-angle unit or swiveling polarizer

(2-18/8) with exceeding threaded bolt
(2-18/8, position a) into the front threaded
hole of the condenser carrier (2-18/1,
position a) by turning the entire wide-angle
unit (or the polarizer). Screw in the unit
until the threaded hole of the holding angle
(2-18/8 position b) lies below the rear
threaded hole (2-18/1, psition b) of the
condenser carrier and contacts the
underside of the condenser.

• Then use the slotted screwdriver to screw

the holding angle to the underside of the
condenser carrier (2-18/1, position b) with
the supplied slotted screw.

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Axiostar plus Inserting white-balance filter Carl Zeiss

2.10 Inserting white-balance filter

The white-balance filter can be used to balance
differing, subjective color perceptions when
observing a specimen and to increase contrast.

☞

The white-balance filter is only for subjective
balancing for the user. For microphotography,
remove the white-balance filter and use an
appropriate conversion filter (e.g. conversion
filter 3200 ... 5500 K).

• Carefully hold the white-balance filter

(2-19/2) on the edge without smudging it
and place it in the luminous field diaphragm
opening (2-19/4).

• To make sure the filter does not fall out,

push the filter clamp (2-19/1) in the groove
(2-19/3) until it engages. A maximum of
two filters can be secured with the filter
clamp.

• There are depressions (2-19/5) on the top of

the luminous field diaphragm (front and
back) to make it easier to remove the filter
clamp and white-balance filter. This allows
you to pry out the filter clamp or filter with
your fingers.

1 Filter clamp
2 White-balance filter
3 Groove
4 Luminous field diaphragm
5 Depression in the luminous field diaphragm

Fig. 2-19 Inserting white-balance filter

☞

If a yellow tone appears when using objectives
5x and 2.5x even though a white-balance filter
is inserted, use an additional neutral filter 25 %
(467849-0000-000) on the luminous field
diaphragm and increase the lamp intensity.

B 40-815 e 12/01 2-21

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Carl Zeiss Attaching epi-fluorescence illuminator Axiostar plus

2.11 Attaching epi-fluorescence

illuminator

The epi-fluorescence illuminator can be used
only in conjunction with a binocular
tube/phototube 30°.

• If necessary, remove the 45° tube or 30°

tupe and adapter for 30° tubes.

• Replace knurled screws on the stage mount

with hexagonal screws (2-20/5).

• Press epi-fluorescence illuminator (2-20/2)

at a slight angle to the right into the spring
of the stand mount.

• Then press the epi-fluorescence illuminator

into the dovetail mount of the stand by
lowering the left side.

• Align epi-fluorescence illuminator to the

outer contours of the stand.

• Loosen the two back clamping screws

(2-20/7) on the epi-fluorescence illuminator.

• First tighten the hexagonal screws (2-20/5)

and then the two back clamping screws
(2-20/7) to hold the illuminator in position.

• Place 30°

ICS tube (2-20/1) with ring mount

in the epi-fluorescence illuminator opening
(2-20/2), align to the outer edges and
tighten the clamping screws (2-20/6) on the
epi-fluorescence.

130° ICS Tube
2 Epi-fluorescence illuminator
3 Filter stop carrier 2
4 Filter stop carrier 1
5 Hexagonal screw (on stand)
6 Clamping screw
7 Clamping screws (back)

Fig. 2-20 Attaching epi-fluorescence illuminator

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Axiostar plus Attaching epi-fluorescence illuminator Carl Zeiss

2.11.1 Filter stop carrier for epi-fluorescence illuminator

The epi-fluorescence illuminator has two slots for filter stop carriers. The carriers can be inserted and
accessed from the right or left.

Filter stop carrier 1 (designated with one dot) has three positions (a, b, c) for filters with a 25 mm
diameter. O-rings are used to hold the filters in the stop carrier.

Filter stop carrier 2 (designated with two dots) also has three positions. Position 1 (adjust) is used to
adjust the fluorescent lamp (see section 2.11.4). Position 2 (open) is a free passage, but a 25 mm
diameter filter can be used. Position 3 (closed) is the locking position for the fluorescent lamp.

• When inserting the filter stop carrier, make sure that the markings on the top and right side of

the carrier match the markings and the epi-fluorescence illuminator.

1 Filter stop carrier 1
2 Filter stop carrier 2
3 Epi-fluorescence illuminator

Fig. 2-21 Filter stop carrier

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Carl Zeiss Attaching epi-fluorescence illuminator Axiostar plus

2.11.2 Attaching HBO 50 fluorescent

lamp

• Place HBO 50 fluorescent lamp (2-22/1) on

the connection supports on the epi­fluorescence illuminator (2-22/3), align and
tighten with clamping screw (2-22/2) .

• Plug the HBO 50 connection cable into the

power supply and plug the power supply
into an outlet.

• Use switch on the front of the power supply

to turn the HBO 50 lamp on and off. The
lamp lights automatically when you turn on
the switch.

1 HBO 50 lamp
2 Clamping screw
3 Epi-fluorescence illuminator

Fig. 2-22 Attaching HBO 50

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Axiostar plus Attaching epi-fluorescence illuminator Carl Zeiss

2.11.3 Replacing burner in fluorescent

lamp

• Turn off power supply for HBO 50. Remove

HBO 50 plug from the socket on the power
supply.

Danger of getting burned! Allow lamp housing
to cool off at least 15 minutes.

• Remove HBO 50 lamp from the epi-

fluorescence illuminator and place it on a
work surface.

• Loosen clamping screw (2-23/1) with Allen

key (SW 3) and lift up lamp housing.

• Press spring handle down (2-23/4) and lift

up heat sink (2-23/2) with the burner
(2-23/3) out of the lamp frame (2-23/5). Set
it down so that clamping screw on the heat
sink is accessible.

The cable on the heat sink cannot be
removed.

• Loosen clamping screw on the heat sink

with the Allen key and remove used burner.

Dispose of used burner according to applicable
ordinances. Pay attention to manufacturer‘s
suggestions.

1 Clamping screw
2 Heat sink
3 Brenner
4 Spring handle
5 Lamp frame

Fig. 2-23 Replacing burner

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Carl Zeiss Attaching epi-fluorescence illuminator Axiostar plus

• Hold the new burner on the labeled metal

base and place it in the heat sink so that
the reflective portion of the burner points
down when placed in the lamp frame. If
both sides are reflective, make sure that the

The heat sink must be parallel to the lamp base.
To aling, press spring handle and turn heat sink
with burner in the lamp frame.

electrode labeled “UP“ is at the top. If there
is a sideways reflective melting position on
the burner, it must point to the side in
relation to the beam path.

• Replace lamp housing and tighten clamping

screws (2-23/1). Make a note of the
number on the operational hours counter.

• Carefully tighten clamping screw on the

heat sink.

Change the burner after reaching the expected
life of 100 hours.

Avoid getting fingerprints on the glass
components of the burner. If fingerprints do
occur, remove immediately.

• Press down spring handle (2-23/4) and

place burner with heat sink into the lamp
frame (2-23/5). Make sure to touch only the
heat sink.

• Slowly let go of spring handle and release

heat sink.

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Axiostar plus Attaching epi-fluorescence illuminator Carl Zeiss

2.11.4 Adjusting burner in fluorescent

lamp

Since the Axiostar plus epi-fluorescence
illuminator has an adjustment guide (filter stop
carrier 2, 2-24/5), the HBO 50 can remain on
the illuminator while adjusting the burner.

• Turn on completely connected fluorescent

lamp via the power supply unit. The burner
will ignite automatically.

If the image of the light arc and the mirror
image are superimposed, the burner will have a
greater thermal load. This will reduce burner
life.

• Move filter stop carrier 2 (2-24/5) to the

adjust position. The light arc of the burner

(lighter) and its mirror image (somewhat
darker) can be seen the filter stop carrier 2
window (2-24/6).

• Use the knob (2-24/4) to focus the collector

so both light arcs are sharply focused.

• Use adjustment screw (2-24/1) to adjust

1 Axial burner adjustment
2 Height adjustment for burner
3 Side adjustment for burner
4 Knob for collector adjustment
5 Filter stop carrier 2 with adjustment guide
6 Image from light arc and mirror image of

burner in the adjustment guide

Fig. 2-24 Adjusting burner

burner in an axial direction to the mirror so
that both light arcs appear the same size in
the adjustment guide window (see Fig.
2-24/6).

• Use the adjustment screws for height

(2-24/2) and side adjustment (2-24/3) to
position the light arc and mirror image
centered and parallel to each other in the
adjustment circle (2-24/6). The light arc and
mirror image should not be superimposed.

• When you are finished making the

adjustments, use the knob to refocus again
and move the filter stop carrier 2 (2-24/5) to
the open or closed position.

B 40-815 e 12/01 2-27

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Carl Zeiss Attaching epi-fluorescence illuminator Axiostar plus

2.11.5 Changing P&C reflector module

The reflector carriage in the epi-fluorescence illuminator must be removed to change the P&C
reflector module.

Removing the reflector carriage:

• Push the reflector carriage lever (2-25/5) to the left or middle position.

• Push up the protective cap (2-25/2) on the epi-fluorescence illuminator (2-25/1) along the groove.

• Push the reflector carriage lever (2-25/5) to the right.

• Turn lock (2-25/3) to horizontal position (open) and pull out the reflector carriage (2-25/4) by the

lock (2-25/3) and place down as shown in Fig. 2-26.

1 Epi-fluorescence illuminator
2 Protective cap
3Lock
4 Reflector carriage
5 Lever for reflector carriage
6 Label for filter combination or pigment

Fig. 2-25 Removing/inserting reflector carriage

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Axiostar plus Attaching epi-fluorescence illuminator Carl Zeiss

Inserting a P&C reflector module:

• Hold the module (2-26/4) with thumb and

index finger as shown in Fig. 2-26 and
insert at a slant from the top with the right
and left holding supports (2-26/5) into the
bottom spring clamps (2-26/2) in the
reflector carriage (2-26/3).

• Press on the top of the module until it

engages in the top spring clamps (2-26/1).

Removing a P&C reflector module:

• Slightly tip the module and disengage first

from the top spring clamps (2-26/1) and
then from the bottom spring clamps
(2-26/2) to remove completely.

Inserting the reflector carriage:

• Turn the reflector carriage (2-25/4) to the

correct insertion position and place in the
epi-fluorescence illuminator. Hold the lever
(2-25/5) to the right and push in the
reflector carriage until it engages.

• Turn lock (2-25/3) clockwise to lock

position.

• Push the reflector carriage lever to the left

or middle position an insert the protective
cap into the groove from above.

1 Top spring clamps
2 Bottom spring clamps
3 Reflector carriage
4 P&C reflector module
5 Holding supports

Fig. 2-26 Removing/inserting P&C reflector module

☞

If the reflector carriage is not locked correctly,
the imaging quality will be affected negatively.

• The last step is to label the three reflector

carriage positions (2-25/6) on the epi­fluorescence illuminator with the included
labels (000000-1100-994) for filter
combination and pigment.

B 40-815 e 12/01 2-29

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Carl Zeiss Attaching epi-fluorescence illuminator Axiostar plus

2.11.6 Changing filter set in P&C

reflector module

You can combine and attach the filter sets for
the P&C reflector module as needed. Filter sets
or completely equipped P&C reflector modules
can be ordered from Carl Zeiss.

• Remove P&C reflector module (2-27/3) from

the reflector revolver and place in a secure
position.

• Unscrew fastening ring (2-27/1) with

mounting sheet (2-27/6) from the tool set.

• Turn over reflector module so that the filter

1 Fastening ring
2 Locking filter
3 P&C reflector module
4 Orientation groove
5 Excitation filter
6 Mounting sheet

(2-27/2 or 5) falls onto a soft surface.

• Insert the locking filter (emission filter)

(2-27/2) and excitation filter (2-27/5) and
secure with the fastening (2-27/1).

Fig. 2-27 Changing filter set in P&C reflector module

The emission filter and excitation filter will be
labeled with an inscription or arrow on the
perimeter. The arrow indicates the installation
direction for the filter into the reflector module
and should point in (see arrow in Fig. 2-27).

To minimize image shift during multiple
fluorescence procedures, the emission filter can
have an additional marking showing the
position of the wedge angle.

The marking should be aligned to the
orientation groove (2-27/4) when inserting an
emission filter in the reflector module. This
guarantees that the wedge angle of the
emission filter has the same defined position in
the reflector modules used. This compensates
for or minimizes the already slight image shift
when Zeiss filter sets are used between
modules.

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Axiostar plus Attaching epi-fluorescence illuminator Carl Zeiss

We recommend the following procedure if you
need to install filters without direction markings
(arrow):

Filters with reflective, dielectric layers should be
installed so that the reflective layer

– Points out for an excitation filter (in relation

to the reflector module)

and

– Points in for an emission filter (Fig. 2-28).

2.11.7 Changing color splitter in P&C

reflector module

Extreme care is required when attaching the
filter and color splitter to avoid damage to the
optical elements and to keep them clean.

We recommend ordering completely equipped
P&C reflector modules since changing the color
splitter has higher requirements.

To replace a color splitter:

• Remove P&C reflector module from

reflector revolver.

• Loosen both slotted screws

(2-29/1) with a screwdriver.

• Hold together both halves of the reflector

module, turn against the installation
position and set aside.

1 Locking filter (emission filter)
2 Reflective layer on emission filter
3 Color splitter
4 Reflective layer on the color splitter
5 Excitation filter
6 Reflective layer on the excitation filter
7 Illumination/Imaging beam path

Fig. 2-28 Inserting filter and color splitter

1 Slotted screws
2 Module half: emission
3 Module half: excitation

Fig. 2-29 Changing color splitter

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Start-Up

Carl Zeiss Attaching epi-fluorescence illuminator Axiostar plus

• Tip the top module half up (Excitation)

(2-30/1) and remove the bottom module
half (Emission) from the holding supports
(2-30/5b).

• Remove color splitter (2-30/2) and spring

frame (2-30/3) from bottom module half.

• Remove old color splitter and carefully place

the new one on the spring frame (2-30/3)
with the reflective side pointing up. Place
both parts together in the bottom module
half. Make sure that the side catch of the
spring frame engages in the correct portion
of the bottom module half.

☞

The reflective (layered) side of the color splitter
has an angled edge or corner (Fig. 2-31).

1 Module half: Excitation
2 Color splitter
3 Spring frame
4 Module half: Emission
5a Eyelets
5b Holding supports

Fig. 2-30 Changing color splitter

• Place Excitation module half (2-30/1) onto

Emission module half (2-30/4) (holding

support 2-30/5b and eyelets 2-30/5a should
connect). Hold both halves together and
turn in the installation position.

• Replace slotted screws and tighten.

• Attach label with filter combination on the

side of the module.

1 Angled edge
2 Angled corner
3 Layered side

Fig. 2-31 Color splitter designation

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Start-Up

Axiostar plus Connecting the instrument to the line Carl Zeiss

2.12 Connecting the instrument to

the line

The microscope can be operated using line
voltages of 100 - 240 V without conversion.

• Connect the line cable with connector

(2-32/2) to the instrument socket (2-32/1)
and connect the earth-contact plug (2-32/3)
to the line.

• Switch on the instrument via the on/off

switch (2-32/4) on the left-hand side of the
instrument.

• The green LED integrated into the on/off

switch lights up to indicate that the
instrument is ready for operation (switch in
"I" position). The integrated halogen lamp
6 V, 20 W must also be on.

1 Instrument socket
2 Line connector
3 Line plug
4 On/off switch

Fig. 2-32 Connecting the instrument to the line

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Start-Up

Carl Zeiss Axiostar plus

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Operation

Axiostar plus Contents Carl Zeiss

OPERATION

Contents

3 OPERATION...............................................................................................................3-3

3.1 Switching on the instrument........................................................................................3-3

3.2 Using the transmitted-light brightfield technique..........................................................3-4

3.2.1 General principle .........................................................................................................3-4

3.2.2 Transmitted-light brightfield configuration...................................................................3-4

3.2.3 Setting transmitted-light brightfield for KÖHLER illumination........................................3-4

3.3 Using transmitted-light phase contrast.........................................................................3-6

3.3.1 General principle .........................................................................................................3-6

3.3.2 Transmitted-light phase contrast configuration.............................................................3-6

3.3.3 Setting transmitted-light phase contrast.......................................................................3-7

3.4 Using the transmitted-light darkfield technique............................................................3-8

3.4.1 General principle .........................................................................................................3-8

3.4.2 Transmitted-light darkfield configuration .....................................................................3-8

3.4.3 Setting transmitted-light darkfield................................................................................3-9

3.5 Using transmitted-light polarization contrast ..............................................................3-12

3.5.1 General principle .......................................................................................................3-12

3.5.2 Transmitted-light polarization contrast configuration..................................................3-12

3.5.3 Setting transmitted-light polarization contrast............................................................3-12

3.5.4 Sample differentiation between gout and pseudogout ...............................................3-13

3.6 Setting epi-fluorescence ............................................................................................3-14

3.6.1 General principle .......................................................................................................3-14

3.6.2 Epi-fluorescence configuration...................................................................................3-14

3.6.3 Setting epi-fluorescence ............................................................................................3-14

3.7 Measurement of lengths............................................................................................3-16

3.8 Attaching ICS double tube.........................................................................................3-17

3.9 Attaching photomicrography equipment....................................................................3-18

3.9.1 Attaching SLR camera, e.g. CONTAX ARIA................................................................3-18

3.9.2 Attaching MC 80 DX microscope camera (film cassette 35 mm) .................................3-20

3.10 Attaching adapters for video cameras ........................................................................3-21

3.11 Attaching adapters for digital compact cameras.........................................................3-23

3.11.1 Digital cameras with 37 mm filter thread, e.g. SONY DCR-PC100 / SONY DSC – S50 ..3-24

3.11.2 Digital cameras with 52 mm filter thread, e.g. SONY DSC–S70, DSC-S75, DSC-S85 ...3-25

3.11.3 Attaching cameras with other filter thread sizes.........................................................3-26

3.11.4 Sony DSC-S70, DC-S75, DSC-S85 on digital camera adapter 44 M52x0.75,

000000-1108-984.....................................................................................................3-26

3.12 Axiostar plus - Packing the mount..............................................................................3-27

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Operation

Carl Zeiss List of illustrations Axiostar plus

List of illustrations

Fig. 3-1 Switching on the instrument........................................................................................3-3

Fig. 3-2 Inserting filters ............................................................................................................3-3

Fig. 3-3 Transmitted-light brightfield for KÖHLER illumination, preparation................................3-4

Fig. 3-4 Setting transmitted-light brighfield for KÖHLER illumination .........................................3-5

Fig. 3-5 Setting transmitted-light phase contrast.......................................................................3-6

Fig. 3-6 Setting Abbe condenser with 5-position turret disk .....................................................3-7

Fig. 3-7 Centering phase stop ..................................................................................................3-7

Fig. 3-8 Setting transmitted-light darkfield................................................................................3-9

Fig. 3-9 Setting transmitted-light polarization contrast...........................................................3-12

Fig. 3-10 Setting epi-fluorescence ............................................................................................3-15

Fig. 3-11 Length measurement.................................................................................................3-16

Fig. 3-12 ICS double tube.........................................................................................................3-17

Fig. 3-13 Attaching SLR camera, e.g. CONTAX ARIA...............................................................3-18

Fig. 3-14 Attaching MC 80 DX microscope camera..................................................................3-20

Fig. 3-15 Attaching Sony "Digital Handycam DCR-PC100" camera ...........................................3-24

Fig. 3-16 Attaching Sony "Digital Still Camera DSC-S70"..........................................................3-25

Fig. 3-17 Inserting the Axiostar plus in the case ........................................................................3-28

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Operation

Axiostar plus Switching on the instrument Carl Zeiss

3 OPERATION

3.1 Switching on the instrument

• Switch on the instrument via the On/Off

switch (3-1/1).

• The green LED integrated in the on/off

switch lights up to indicate that the
instrument is ready for operation (switch in
"I" position). The integrated 6 V 20 W long­life halogen lamp must also be on.

☞

The Axiostar plus microscope is supplied with
factory-aligned illumination. The illumination
need not be adjusted even when the lamp is
exchanged by the customer.

• Set the required brightness via the

brightness control (3-1/2).

1 On/off switch with integrated control lamp
2 Brightness control

Fig. 3-1 Switching on the instrument

• Depending on the application, place one or

several of the following dia. 32 filters
(3-2/2) on the dust-protection glass of the
luminous-field diaphragm (3-2/1) , e.g.

– Interference wide-band filter, green, 32 x 4,

for contrast enhancement in b/w
photography of stained sections and for
phase contrast.

– CB 3 conversion filter, d = 32 x 2, to

generate the correct color temperature of
3200 K when artificial light color reversal
film is used.

– CB 3, d = 32 x 2, and CB 12, d = 32 x 2,

conversion filters for use with daylight color
film.

• If necessary, use the filter clamp (3-2/3) to

secure the filter.

When the LED illuminator is used instead of a
condenser, it must be switched on via the
toggle switch, and the illumination intensity
must be set via the control. Setting Köhler
illumination is not required on account of the
homogeneous field illumination up to diameter
20 mm. Only when objective 2.5x is used, it
may be necessary to center the LED illuminator
and to slightly lower the condenser carrier.

1 Luminous-field diaphragm with dust protection glass
2 Filter
3 Filter clamp

Fig. 3-2 Inserting filters

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Operation

Carl Zeiss Transmitted-light brightfield Axiostar plus

3.2 Using the transmitted-light

brightfield technique

3.2.1 General principle

Transmitted-light brightfield microscopy is the
most usual of all the optical microscopy
techniques, since it allows the easy and fast
viewing of high-contrast or stained specimens
(e.g. blood smears).
In order to obtain optimum resolution with full
illumination of the field, the condenser, the
luminous-field diaphragm and the aperture
diaphragm must be set in accordance with the
rules of the KÖHLER illumination principle.
Here, the illumination cone is adapted to the
objective's opening cone. In this way, the
numerical aperture of the optical system is
used, and "superfluous" light, which can cause
interference in the form of scattered light, is
prevented.

3.2.2 Transmitted-light brightfield

configuration

– Each Axiostar plus microscope can be

configured to permit the transmitted-light
brightfield technique.

– When using the Abbe condenser with 5-

position turret, set the brightfield position
(H) via the turret disk.

– If a slider for phase contrast is available with

the 0.9/1.25 Abbe condenser, it must be
removed when using the transmitted-light
brightfield technique.

3.2.3 Setting transmitted-light

brightfield for KÖHLER
illumination

Requirement:

– As described in section 2, the microscope is

ready for operation and switched on
according to section 3.1.

1 Brightness control
2 Aperture diaphragm lever
3 Condenser drive

Fig. 3-3 Transmitted-light brightfield for KÖHLER

illumination, preparation

Settings:

• First, place a high-contrast specimen with

0.17 mm cover slip on top of the
mechanical stage 75x30 R/L.

• Set image brightness using the brightness

control (3-3/1) on the microscope stand.

• Move the Abbe condenser 0.9/1.25 to the

upper stop position via the condenser drive
(3-3/3) and move aperture diaphragm lever
(3-3/2) to the center position.

☞

If the microscope is equipped with a mechanical
stage 75 x 30 R (stage drive on the right side),
then the condenser drive will be attached on
the left in the factory.

The condenser drive will be located on the right
side of the microscope if the microscope is
equipped with a mechanical stage 75 x 30 L.

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Operation

Axiostar plus Transmitted light brightfield Carl Zeiss

• Swivel 10x objective into beam path via

knurled ring (3-4/5) of the nosepiece.

• Look through the fixed eyepiece of the

binocular tube (3-4/6) first and focus on the
object via the focusing drive (3-4/1).

• Then set the focus for the other eye by

turning the eyelens of the focusing eyepiece
if necessary.

• Close luminous-field diaphragm (3-4/2) until

it is visible in the field of view, even if not in
focus (3-4/A).

• Use the condenser (3-3/3) to adjust the

condenser until the luminous-field
diaphragm is sufficiently in focus (3-4/B)

• Center luminous-field diaphragm (3-4/C)

using both centering screws (3-4/4) and
then open it until the edge of the
diaphragm just disappears from the field of
(3-4/D).

• For aperture diaphragm setting (contrast),

remove one eyepiece from the tube and
look into the tube with your naked eye. Use
lever (3-4/3) to set the aperture diaphragm
to approx. 2/3 ... 4/5 of the diameter of the
objective exit pupil (3-4/E). In most
applications, this setting of the aperture
diaphragm provides optimum contrast at
almost full resolution and therefore the best
compromise for the human eye.

• Replace eyepiece into the tube.

1 Focusing drive
2 Luminous field diaphragm
3 Lever for aperture diaphragm setting
4 Centering screws for condenser
5 Knurled ring - nosepiece
6 Binocular tube

Fig. 3-4 Setting transmitted-light brighfield for

KÖHLER illumination

☞

The size of the field of view and objective
aperture changes every time an objective is
switched; therefore, you must reset field of
view and aperture settings for optimal results.
For objectives < 4x, the swivel wide-angle unit
must be moved into the light path (see
section 2.9).

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Operation

Carl Zeiss Transmitted-light phase contrast Axiostar plus

3.3 Using transmitted-light phase

contrast

3.3.1 General principle

The phase contrast technique is ideal for
examinations of thin, unstained specimens, e.g.
culture cells.

The phase contrast technique uses the optical
modulators "phase stop and phase ring" and
the interference procedures during the
formation of the intermediate image to change
the small phase differences in intensity and
color differences which are visible to the human
eye.

☞

Optimum phase contrast requires a high level of
cleanliness! Therefore, clean the front lens of
the objective used, the visible condenser
surfaces, the upper cover slip surface and the
lower carrier plate surface of the specimen with
particular care and carefully remove grease.

1Eyelens – auxiliary microscope
2 Knurled ring – auxiliary microscope
3 Auxiliary microscope
4 Binocular tube/phototube
5 Hexagonal key SW 2
6 Centering screws for annular diaphragm centering
7 Luminous field diaphragm
8 Slider for phase contrast

9 Lever for aperture diaphragm
10 Phase contrast objective
11 Diopter

Fig. 3-5 Setting transmitted-light phase contrast

3.3.2 Transmitted-light phase contrast

configuration

– Abbe condenser 0.9/1.25 and slider for

phase contrast, e.g. Ph 2.

– When using the Abbe condenser with 5-

position turret, set the relevant phase stop
position (Ph1, Ph2 or Ph3) via the turret
disk.

– Phase contrast objectives with phase rings

Ph 1, Ph 2 or Ph 3 for different average
numeric apertures which can also be used
in brightfield without any restriction.

– The name of the phase stop on the slider

for phase contrast must correspond to the
relevant name on the objective, e.g. Ph 1.

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Operation

Axiostar plus Transmitted-light phase contrast Carl Zeiss

3.3.3 Setting transmitted-light phase

contrast

Requirements:

– As described in chapter 2, the microscope is

ready for operation and switched on
according to section 3.1.

– The microscope must be set for

transmitted-light brightfield, as described in
section 3.2.

Settings:

• Swivel phase contrast objective, e.g.

40×/0.65 Ph 2 (3-5/10), into the beam path.

• Open luminous-field diaphragm (3-5/7) and

aperture diaphragm (3-5/9) or (3-6/3) on
the Abbe condenser with turret.

If a phase stop position (or darkfield position) is
set on the turret condenser, a green dot
appears in the window of the aperture
diaphragm indicating that the stop must be
opened completely (green dot on the aperture
diaphragm and in the window (3-6/4) are in the
same row.

• On the condenser, insert the slider for

1 Turret disk
2 Window for turret disk position
3 Aperture diaphragm lever
4 Window for aperture diaphragm marking
5 Adjusting screws for phase stops

Fig. 3-6 Setting Abbe condenser with

5-position turret disk

phase contrast (3-5/8) with the same name
as on the phase contrast objective, e.g.
Ph 2, or set the relevant position on the
turret disk (3-6/2) of the Abbe condenser
with 5-position turret.

• Adjust the brightness.

• Check whether phase stop centering

complies with the figure (3-7). For this,
remove one eyepiece and replace it with the
diopter (3-5/11) or a auxiliary telescope
(3-5/3). Focus on the phase ring by pulling
out or pushing in the 3-5/1) of the auxiliary
telescope via the knurled ring (3-5/2).

Fig. 3-7 Centering phase stop

• If required, center the phase stop (3-7/A)

via the two adjusting screws (3-5/6 or
(3-6/5) using a 90° hexagonal key SW 1.5.

• Then replace the diopter or the auxiliary

telescope with the eyepiece.

☞

Complete phase contrast is only achieved if the
bright phase stop (in the condenser) and the
dark phase ring (in the objective) are exactly
congruent in the illumination beam path
(3-7/B).

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Operation

Carl Zeiss Transmitted-light darkfield Axiostar plus

3.4 Using the transmitted-light

darkfield technique

3.4.1 General principle

Darkfield is mainly used for small or minute
objects such as bacteria, but also for emulsions
or unstained objects in watery solutions.

In transmitted-light brightfield, unstained
biological specimens, such as bacteria or living
cell cultures, are often barely identifiable – if at
all – on account of their light transmission. The
situation changes significantly if such specimens
are viewed in transmitted-light darkfield. In
principle, the specimen is illuminated with an
illumination aperture which is larger than that
of the objective used.

Only the diffracted and scattered light
components which are so important for image
production reach the objective, while the
directly reflecting light bundles are guided past
the objective.

This is one of the reasons why even fine
structures can be resolved and appear bright on
a dark background although they partially lie
below the resolving power of the light
microscope.

3.4.2 Transmitted-light darkfield

configuration

– Condenser with slider for darkfield.
– When using the Abbe condenser with

5-position turret, set the darkfield position
(DF, 0.65 / 0.9) via the turret disk.

– ICS objectives with a numerical aperture

smaller than that of the used darkfield stop
can be used.

– For further details, please see the following

table:

Condenser with slider for contrasting techniques Suitable objectives

Slider for darkfield 0.25/0.9 (DF 10) CP-Achromat 5x/0.12

DF setting on condenser revolver (0.65/0.9) CP-Achromat 10x/0.25

A-Plan 5x/0.12

A-Plan 10x/0.25

Achroplan 4x/0.10

Plan-Neofluar 2.5x/0.075

Slider for darkfield 0 .65/0.9 (DF 40) CP-Achromat 10x/0.25

DF setting on condenser revolver (0.65/0.9) CP-Achromat 40x/0.65

A-Plan 10x/0.25

A-Plan 20x/0.45

Achroplan 10x/0.25

Achroplan 20x/0.45

Achroplan 40x/0.65

Slider for phase contrast Ph 1 All engraved Ph 1

Slider for phase contrast Ph 2 All engraved Ph 2

Slider for phase contrast Ph 3 All engraved Ph 3

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Operation

Axiostar plus Transmitted-light darkfield Carl Zeiss

3.4.3 Setting transmitted-light

darkfield

Before starting:

– As described in section 2, the microscope is

ready for operation and switched on
according to section 3.1.

Settings:

• Set KÖHLER illumination in the same way as

for transmitted-light brightfield, e.g. with
objective 10x.

• Insert the relevant slider (3-8/3) for darkfield

− see table on page 3-8 − into the opening

on the condenser until stop.

• Open the luminous-field diaphragm (3-8/2).

• Increase the brightness setting on the

potentiometer.

• If the image center is too bright or too dark,

sensitively correct the condenser height
until the field of view appears
homogeneously dark or features
homogeneous brightness distribution.

☞

Darkfield specimens require a considerably
higher level of cleanliness than specimens for
other methods; fingerprints, dirt and dust in
particular brighten the background and reduce
the contrast of the object image.

For darkfield setting, it is also possible to use
the slider for phase contrast 3 and the
following objectives instead of the slider for
darkfield 0.25/0.9: CP-Achromat, A-Plan and
Plan-Neofluar 2.5x – 10x and Achroplan 4x.

Darkfield condensers can be placed directly in
the Axiostar plus when you use the darkfield
condenser carrier. Chapter 2 offers an overview
of applicable types and recommended
objectives.

1 Focusing drive
2 Luminous field diaphragm
3 Dark field slider
4 Aperture diaphragm lever
5 Centering screws for condenser
6Nosepiece
7 Binocular tube

Fig. 3-8 Setting transmitted-light darkfield

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Operation

Carl Zeiss Transmitted-light darkfield Axiostar plus

Setting darkfield contrast with dry
darkfield condensers

• If necessary, swivel open the wide-angle

unit, colored disk carrier, polarizer or λ
plate.

• Move condenser carrier down until it makes

contact.

• Place dry darkfield condenser in darkfield

condenser carrier (see section 2).

• Place darkfield condenser carrier in

condenser carrier and center approximately
so that when the condenser carrier moves
the condenser moves into the gap of the
mechanical stage without making contact.

• Move condenser up until it makes contact.

• Place specimen.

• Set lamp brightness to maximum.

• Swivel in objective with a small

magnification (e.g. 5x or 10x) and focus the
specimen with the focusing drive.

• Reset focus level with Z drive. Focus the

luminous field diaphragm with the
condenser drive. Open the luminous field
diaphragm just above the viewing field
edge.

• Optimize the contrast with the condenser

drive, if necessary.

Additional information:

Darkfield specimens require a considerably
higher level of cleanliness than specimens for
other methods; fingerprints, dirt and dust in
particular brighten the background.

• Since you can only see the specimen where

small components illuminate when using
darkfield illumination, set the specimen so
that an even detail distribution can be seen.
This will make the imaging of the luminous
field diaphragm easier to identify.

• Close luminous field diaphragm by turning

luminous field diaphragm ring
counterclockwise until it makes contact.

• Lower the condenser until the edge of the

luminous field diaphragm is displayed
clearly (luminous field diaphragm focus
level). There will be an increasing or
decreasing light ring above or below the
focus level of the luminous field diaphragm
(circular “breathing” of the luminous field
diaphragm depiction).

• Recenter the luminous field diaphragm with

the adjustment screws on the condenser
carrier.

• Swivel in desired objective.

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Operation

Axiostar plus Transmitted-light darkfield Carl Zeiss

Setting darkfield contrast with immersion
oil darkfield condensers

• If necessary, swivel open the wide-angle

unit, colored disk carrier, polarizer or λ
plate.

• Move condenser carrier down until it makes

contact.

• Place immersion oil darkfield condenser in

darkfield condenser carrier (see section 2).

• Place darkfield condenser carrier in

condenser carrier and center approximately
so that when the condenser carrier moves
the condenser moves into the gap of the
mechanical stage without making contact.

• Move condenser up until it makes contact.

• Place a drop of immersion oil (without

bubbles, if possible) on the center of the
condenser.

• Place specimen. The immersion oil will

disperse due to the capillary effect between
the top of the condenser and the bottom of
the specimen holder.

• Slightly move the mechanical stage back

and forth to dissipate any air bubbles in the
immersion oil.

• Set lamp brightness to maximum and open

luminous field diaphragm completely.

• Swivel in objective with a small

magnification (e.g. 10x) and focus the
specimen with the focusing drive. Then step
by step swivel in the next largest dry
objectives and focus the specimens with the
focusing drive.

• Close luminous field diaphragm by turning

luminous field diaphragm ring
counterclockwise until it makes contact.

• Lower the condenser until the edge of the

luminous field diaphragm is displayed
clearly.

• Recenter the luminous field diaphragm with

the adjustment screws on the condenser
carrier.

The luminous field diaphragm appears only as a
circle segment on the edge of the viewing field
due to the high magnification of the immersion
oil objective. As a result, focusing and centering
of the luminous field diaphragm must be
repeated. If necessary, the luminous field
objective should be opened slightly if the light
intensity is too small.

The luminous field diaphragm is centered when
the edge of the luminous field diaphragm is
centered or equidistant from the viewing field
edge.

• For a sharply focused specimen, open the

sharply set luminous field diaphragm until
just above the viewing field edge.

• You can improve the contrast of the

microscope image by slightly adjusting the
focus level of the condenser with the
condenser drive.

• The last criteria is an equally dark

background of the eyepiece image.

• For immersion oil objectives with an iris

diaphragm, the contrast can be further
optimized by turning the adjustment of the
iris diaphragm.

• Center the luminous field diaphragm on the

condenser carrier with the adjustment
screws and focus with the condenser drive.

• Place one drop of immersion oil on the

specimen location, swivel in immersion oil
objective and focus specimen.

Additional information:

Darkfield specimens require a considerably
higher level of cleanliness than specimens for
other methods; fingerprints, dirt and dust in
particular brighten the background.

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Operation

Carl Zeiss Transmitted-light polarization Axiostar plus

3.5 Using transmitted-light

polarization contrast

3.5.1 General principle

The transmitted-light polarization technique is
used with specimens that change the
polarization state of light. Such specimens, e.g.
crystals, minerals or polymers, are termed as
birefringent. If birefringent substances are
viewed between crossed polarizers (polarizer ⊥
analyzer), they appear bright while their
surrounding remains dark.

Birefringent substances are recognized by the
fact that they display 4 bright and 4 dark
positions between crossed polarizers after
rotation of the specimen around 360°.
Depending on the level of birefringence,
thickness and orientation of the object,
interference colors will occur from gray (usually
in biological objects) to white, yellow, red and
blue. These interference colors can be of 1

st

or

higher order.

3.5.2 Transmitted-light polarization

contrast configuration

– Polarizer, oriented in EAST-WEST direction,

mounted on swivel-out carrier (3-9/1).

– Analyzer, oriented in NORTH-SOUTH

direction (3-9/2).

1 Polarizer on swivel-out carrier
2 Analyzer

Fig. 3-9 Setting transmitted-light polarization

contrast

3.5.3 Setting transmitted-light

polarization contrast

Before starting:

– As described in section 2, the microscope is

ready for operation and switched on
according to section 3.1.

– The microscope must be set for

transmitted-light brightfield, as described in
section 3.2.

Settings:

• Screw NORTH-SOUTH-oriented analyzer

(3-9/2) into the tube (removed from the
stand) from below. NORTH-SOUTH
orientation is available when the marking
on the analyzer is aligned along the
longitudinal axis of the stand.

• Swivel polarizer (3-9/1) into the beam path;

the field of view appears dark because of
the crossed polarizers.

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Operation

Axiostar plus Transmitted-light polarization Carl Zeiss

• If necessary, correct the orientation of the

analyzer to achieve an optimal, dark
viewing field.

• Move the object to be examined into the

field of view. Birefringent (anisotropic)
objects now usually exhibit the optical
effects described above.

☞

The orientation of the analyzer to the polarizer
changes when you turn the tube, which in turn
influences the contrast.

3.5.4 Sample differentiation between

gout and pseudogout

• Move two polarizers to the dark position

(analyzer NORTH-SOUTH-oriented, polarizer
EAST-WEST-oriented).

• Swivel in lambda plate and, if a rotary

lambda plate is available (e.g. 445226­0000-000), set the oscillation direction to
45° (γ, stop position).

• Select crystal needles that are oriented in

the gamma direction (see marking on the
lambda plate).

Analysis:

• If the crystal needles oriented parallel to the

gamma direction of the lambda plate are
yellow, and the crystal needles lying at a
right angle to the gamma direction are
blue, the crystals are monosodium urate
crystals (gout).

• If the crystal needles oriented parallel to the

gamma direction of the lambda plate are
blue, and the crystal needles lying at a right
angle to the gamma direction are yellow,
the crystals are calcium pyrophosphate
crystals (pseudogout).

This analysis is also possible using a polarizer
with cemented lambda plate which can be
placed on the luminous-field diaphragm. In that
case, the lambda plate needs not be rotated.

B 40-815 e 12/01 3-13

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Operation

Carl Zeiss Setting epi-fluorescence Axiostar plus

3.6 Setting epi-fluorescence

3.6.1 General principle

The epi-fluorescence method displays
fluorescent substances with strong contrast in
typical fluorescent colors. In an epi-fluorescence
microscope, this occurs through a light
generated via a heat protection filter on the
excitation filter (bandpass). The filtered short­wave excitation beam is reflected by a
dichromatic beam splitter and focused on the
specimen via the objective. The specimen
absorbs the short-wave beam and emits a long­wave fluorescence beam (Stoke’s Law), which is
registered by the objective and let through by
the dichromatic beam splitter. Then the beams
pass through a blocking filter
(lowpass/bandpass) that only allows long-wave
beams emitted from the specimen to pass.

The excitation and blocking filters must be
calibrated spectrally; they are both in the
reflector module FL P&C, along with the
dichromatic beam splitter.

You can get an overview of filter sets and
fluorochromes available from Zeiss at:

www.zeiss.de/micro

“Fluorescent microscopy” under
“Techniques of Microscopy”

3.6.2 Epi-fluorescence configuration

• Recommended objectives Plan-Neofluar or

Fluar (UV excitation).

• Epi-fluorescence illumination with reflector

module FL P&C.

• Mercury vapor short-arc lamp HBO 50 for

incident illumination.

☞

The mercury vapor short-arc lamp must be
adjusted with the adjustment guide before
using it for the epi-fluorescence procedure. If
necessary, you must readjust the setting
depending on the amount of use.

3.6.3 Setting epi-fluorescence
Before starting:

– The microscope is ready for use as outlined

in section 2.

Settings:

• Turn on the halogen lamp with the line

switch on the microscope.

• Swivel the desired objective into place.

• Search for the specimen location to be

observed in the transmitted light. If the 5­position Abbe condenser with turret is to be
used, set the turret to position H
transmitted-light brightfield (or phase
contrast).

• Set KÖHLER illumination in the same way as

transmitted-light brightfield.

• Focus on the specimen with the focusing

drive.

• Turn off halogen lamp.

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Operation

Axiostar plus Setting epi-fluorescence Carl Zeiss

The first epi-fluorescence setting is significantly
easier if you use the Plan-Neofluar 20x/0.50
objective and a strong fluorescent specimen.
Demonstration specimens can be used first.

• First leave the illumination beam closed;

move the filter stop carrier 2 (3-10/2) to the
closed position.

• Turn on the HBO 50 with the power supply

and let it warm up to operating
temperature for about 15 minutes.

• Use the adjustment handle (3-10/3) to

move the desired fluorescence filter
combination on the reflector carrier into the
beam path.

• Turn off the halogen lamp (on microscope)

and open illumination beam path. Move the
filter stop carrier 2 to the open position.

• Refocus on the specimen and, if necessary,

optimize the collector setting with the knob

1 Knob for collector adjustment
2 Filter stop carrier 2
3 Adjustment handle

Fig. 3-10 Setting epi-fluorescence

(3-10/1) on the HBO 50. Set the collector so
that the illumination from the field of view
appears as equal as possible to the short­wave excitation from the reflector module.
Correction of the collector position is no
longer necessary for modules with long­wave excitation.

B 40-815 e 12/01 3-15

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Operation

Carl Zeiss Measurement of lengths Axiostar plus

3.7 Measurement of lengths

Before starting:

The measurement of lengths using the Axiostar
plus requires the following, for example:

– stage micrometer, positive 5 + 100/100 y

D = 0.17 mm

– eyepiece crossline micrometer 10 : 100,

d = 26 mm

– An overview of available stage micrometers

and eyepiece reticles is provided in
chapter 1.6.

☞

The distance to be measured should be ≥ 5 mm
in the intermediate eyepiece image in order to
keep the influence of random measuring
deviations as low as possible.
Other measuring errors can occur if the
eyepiece has not been inserted into the tube
until stop.

Settings:

Before the length measurement using the
microscope can be performed, the micrometer
or scale value of the used objective / eyepiece
reticle combination must be determined. This
scale value is exactly that distance in the
specimen which complies to one interval of the
used eyepiece crossline micrometer.

For calibration, align the scales of the stage
micrometer and the crossline micrometer
parallel to each other by turning the eyepiece,
and make the zero lines of both scales exactly
congruent.

If, for example, 99 increments (of 10 µm each)
of the stage micrometer correspond to exactly
100 increments of the crossline micrometer, as
in Fig. (3-11), the resulting scale value k’ for the
used objective / eyepiece reticle combination
(A-Plan 10x/0.25 and crossline micrometer
10:100) is

99

k‘ = x × 10 µm = 9.9 µm

100

Fig. 3-11 Length measurement

After exchange of the stage micrometer for the
specimen to be measured, the measuring
distance of interest L results from the number
of increments of the eyepiece crossline
micrometer (tenth estimated) multiplied with
the scale value k’:

L = 35.5 × 9.9 µm = 351.5 µm.

Particularly large object structures can also be
determined by using the vernier scale
gradations (0.1 mm) on the mechanical stage.
Here, it might be necessary to determine the
distance to be measured through calculation
from a combined x and y measurement
(Pythagoras).

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Operation

Axiostar plus Attaching

ICS double tube Carl Zeiss

3.8 Attaching

ICS double tube

• Loosen knurled screw (3-12/4) on the stand

and remove binocular tube, if available.

• Remove dust cover from the underside of

the tube.

• Press the

ICS double tube (3-12/1) to the

right in a slightly inclined position into the
spring of the stand mount.

• Then set the double tube

ICS by lowering

the left side into the dovetail of the stand
mound. This will secure the double tube

ICS

from falling out.

• Turn the

ICS double tube into the required

viewing direction and use the knurled screw
(3-12/4 or hexagonal screw) to clamp it into
the threaded opening (3-12/5). Both the
illustrated rotation and the 90° backward
rotation are possible. Accordingly, the
controls of the

ICS double tube are

accessible to the user from the side or from
the front.

• Remove dust caps from the eyepiece tubes

and insert two eyepieces each (3-12/2) with
the same field number 18 or 20.

• Insert country-specific adapter (3-12/9) into

the power unit (3-12/8).

• Insert angled connector (3-12/10) of the

power unit into the 12 V 3-12/3) of the ICS
double tube.

• Use the enclosed self-adhesive cable holders

(3-12/11) to suitably attach the cable to the
stand and guide it to the back.

• Connect power unit to the line.

The ICS double tube is operated via:

• Adjusting lever (3-12/7) for the positioning

of the light pointer in the microscope image

• Control (3-12/6) to set the brightness of the

light pointer.

1 ICS double tube
2 Eyepieces
3 12 V socket
4 Knurled screw (or hexagonal screw)
5 Threaded opening for knurled screw or hexagonal

screw
6 Control for intensity of light pointer
7 Adjusting lever for light pointer
8 Power unit
9 Country-specific adapter
10 Angled connector
11 Cable holder

Fig. 3-12 ICS double tube

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Operation

Carl Zeiss Attaching photomicrography equipment Axiostar plus

3.9 Attaching photomicrography

☞

For the use of the photomicrography equip­ment, please observe the relevant separate
manuals in addition to the information provided
in this manual.

The Axiostar plus microscope with phototube
can be changed from observation to photo­micrography via the control knob (3-13/7 and
3-14/8) attached to both sides of the

equipment

phototube (photomicrography position: turned
counterclockwise until stop: 100 % doc). In the
other position of the knob, the beam path is
directed to the eyepieces (100 % vis). Special
adapters allow commercially available 35 mm
SLR cameras and special microscope cameras
(e.g. MC 80

DX) to be attached to the camera

port of the Axiostar plus.
If focusing is not to be made via the viewfinder

of the camera, the component with the
eyepiece reticle must be screwed in the
eyepieces (see sections 1.6 and 2.5.1).

• For focusing, set the eyepiece until the

reticle is imaged in focus.

1 Cable release
2 Camera housing
3 T2-Adapter for CONTAX bayonet

42.5× connector for T2
5 Phototube
6 Hexagonal screw
7 Control
8 Dust protection cap

• Then focus until both the microscope image

and the reticle are in focus.

3.9.1 Attaching SLR camera,

e.g. CONTAX ARIA

• Screw T-2 adapter (3-13/3) for the CONTAX

bayonet on the 2.5x connector for T2
(3-13/4) (456005-0000-000).

• Attach camera housing (3-13/2) and cable

release (3-13/1), if required.

• Loosen three hexagonal screws (3-13/6)

remove the dust cover (3-13/8) from the
phototube (3-13/5) and insert the
premounted unit (3-13/A) in the phototube.

• Align the camera unit in the required

position and tighten the three hexagonal
screws (3-13/6).

• For photomicrography, turn control (3-13/7)

counterclockwise until stop. When using
the phototube 30°

ICS, pull out the lever for

light path switching completely (100 %
light to camera port).

Fig. 3-13 Attaching SLR camera,

e.g. CONTAX ARIA

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Operation

Axiostar plus Attaching photomicrography equipment Carl Zeiss

When artificial light color reversal film is used, the CB 3 conversion filter provides the correct color
temperature of 3200 K. The filter must be placed on the dust cover of the luminous-field diaphragm
(3-2/2), as mentioned in chapter 3.1.

For daylight color reversal film, the CB 12 conversion filter must be used in addition to the CB 3
conversion filter.

Various T2 adapters for SLR cameras are listed below:

T2 adapters for SLR camera housings Cat. No.

T2 adapter for CONTAX (CONTAX bayonet) 416010-0000-000

T2 adapter for OLYMPUS OM (OM bayonet) 416002-0000-000

T2 adapter for MINOLTA (SR bayonet) 416003-0000-000

T2 adapter for CANON (FD bayonet) 416004-0000-000

T2 adapter for NIKON (F bayonet) 416009-0000-000

T2 adapter for PENTAX (KA bayonet) 416011-0000-000

☞

For detailed information on SLR cameras, please see the new operating manual B 40-046 e,
“Photomicrography using 35 mm SLR cameras”, from Carl Zeiss.

B 40-815 e 12/01 3-19

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Operation

Carl Zeiss Photomicrography equipment Axiostar plus

3.9.2 Attaching MC 80 DX microscope

camera (film cassette 35 mm)

• Loosen hexagonal screws (3-14/7) and

remove dust protection cap (3-14/9) from
the phototube.

• Insert adapter 60 for microscope camera

(3-14/5) (456006-0000-000) into phototube
(3-14/6) and fix it using three hexagonal
screws (3-14/7).

• Insert P 2.5× projection lens (3-14/4) into

adapter 60 for microscope cameras
(3-14/5).

1 35 mm Mot DX film cassette
2 MC 80
3 Clamping ring
4P 2.5× projection lens
5 Adapter 60 for microscope cameras
6 Phototube
7 Hexagonal screws
8 Control
9 Dust protection cap

Fig. 3-14 Attaching MC 80

DX basic body

DX microscope

camera

• Attach MC 80

DX basic body (3-14/2) onto

adapter 60 for microscope cameras until
stop, align it and fix it by turning clamping
ring (3-14/3) anti-clockwise.

• Attach 35 mm Mot DX film cassette

(3-14/1) to the basic body in such a way
that the contact pins firmly engage in the
relevant sockets.

• For microphotography, turn control knob

(3-14/8) counterclockwise until stop. When
using the phototube 30°

ICS, pull out the

lever for light path switching completely
(100 % light to camera port).

When artificial light color reversal film is used,
the CB 3 conversion filter provides the correct
color temperature of 3200 K. The filter must be
placed on the dust cover of the luminous-field
(3-2/2), as mentioned in chapter 3.1.

For daylight color reversal film, the CB 12
conversion filter must also be used. The filter
must be placed on the dust cover of the
luminous-field diaphragm (3-2/2), as described
in chapter 3.1.

☞

For detailed information on the MC 80 DX,
please see manual B 40-036-d, “Microscope
Camera MC 80

DX.”

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Operation

4

Axiostar plus Attaching adapters for video cameras Carl Zeiss

3.10 Attaching adapters for video

cameras

The following video adapters and video zoom
adapters with interface 60 permit the

and 3-chip CCD cameras to the phototube of
the Axiostar plus.

attachment of 1-chip b/w and color cameras

Tube Adapter Cameras

456106

456106-0000-000

60
C 1/2“

0.5

×

CAMERAS

WITH

C-MOUNT

3-CHIP

CAMERAS
WITH 2/3“

BAYONET

AXIOSTAR

PLUS

PHOTO-

TUBE WITH
INTERFACE

60 MM

456105-0000-000

456105

60
C 2/3“

×

1.0

456108

456108-0000-000

60
C 1/3“

×

0 .4

456115-0000-000

456115

60
ENG 2/3“

×

1 .0

000000-1069-414

456107

60
C 2/3“

0.63

452989-0000-000

Zoom 44 C 1/3“
(3 CCD)
0 .35x ... 1 .6x

To be used with:

456140-0000-000

×

3-CHIP

452984-0000-000

456124

Zoom 44
ENG 1/2“

0 .5× ... 2 .4×

To be used with:

456140-0000-000

452992-0000-000

44
ENG 1/2“

0 .63×

56118

To be used with:

456140-0000-000

CAMERAS
WITH 1/2“

BAYONET

B 40-815 e 12/01 3-21

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Operation

Carl Zeiss Attaching adapters for video cameras Axiostar plus

The connecting piece 60 - 44 also allows video
adapters with 44 mm interface to be used with

Video adapter

(Cat. No.)

456140

456140-0000-000

Connecting piece 60 - 44

Microscopes with interface 60 mm and
all video adapters for 44 mm interface.

Suitable for Comments

☞

The instructions of the camera manufacturer
must also be observed when operating the
video camera.

Attachment and settings:

• Loosen three hexagonal screws and remove

dust cover from the camera tube of the
Axiostar plus

• Screw video adapter or video zoom adapter

with C-mount thread into the video
camera.

• Insert video adapter or video zoom adapter

in ENG 2/3" or ENG 1/2“ bayonet of the
video camera and clamp it tight.

• Insert premounted unit (video camera with

video adapter or video zoom adapter) in
camera tube of the Axiostar plus, align it
and fix it using the three hexagonal screws.

.

the phototube of the Axiostar plus with 60 mm
interface.

Connects video adapters for 44 mm
interface to microscopes with 60 mm
interface.

• Insert eyepiece with photo reticle in the

binocular tube and align photo reticle
parallel to the camera.

• Swivel out folding prism on the binocular

phototube 45° ICS to direct 100 % of the
light to the port. When using the
phototube 30° ICS, pull out the lever for
light path switching completely (100 %
light to camera port).

• Set the required zoom magnification factor

via the wheel of the video zoom adapter.

• If required, adjust image brightness on the

monitor by changing the lamp brightness
on the microscope stand.

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Operation

Axiostar plus Attaching adapters for digital compact cameras Carl Zeiss

3.11 Attaching adapters for digital

compact cameras

Tube Adapter Cameras

AXIOSTAR

PLUS

PHOTO-

TUBE WITH
INTERFACE

60 MM

000000-1096-522

D40
M52x0.75

To be used with:

456006-0000-000

000000-1108-984

44
M52x0.75

To be used with:

456140-0000-000

COMPACT

DIGITAL

CAMERAS /

CAMCOR-

DERS WITH

FILTER

THREAD

37/52 MM

B 40-815 e 12/01 3-23

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Operation

Carl Zeiss Attaching adapters for digital compact cameras Axiostar plus

3.11.1 Digital cameras with 37 mm

filter thread, e.g. SONY DCR­PC100 / SONY DSC – S50

When delivered, the sliding mount with thread
M37 (3-15/2) and the lens mount (3-15/4) are
premounted. The drawing on the right shows
the disassembled components.

• Remove threaded adapter ring M37/M52

(3-16/3).

• Plug connector 60 (3-15/6) onto the

binocular phototube (3-15/7) and tighten
the 3 set screws (3-15/8).

• Screw the unit, consisting of sliding mount

with thread M37 (3-15/2) and lens mount
(3-15/4), into the M37 filter thread of the
camera (3-15/1).

• With the lens mount (3-15/4) pointing

forward, plug the unit onto connector 60
(3-15/6) until stop and tighten the set
screw (3-15/5).

1 SONY DCR-PC100 camera
2 Sliding mount with thread M37
3 Set screw
4 Lens mount
5 Set screw
6 Connector 60 for microscope camera
7 Binocular phototube
8 Set screw

Fig. 3-15 Attaching Sony "Digital Handycam DCR-

PC100" camera

Depending on the microscope configuration
and the camera used, the distance between
the camera lens and the lens mount (3-15/4)
must perhaps be optimized (see double arrow).
This is required in particular if an untrimmed
image cannot be achieved in any zoom
position of the camera lens.

Make the following settings on the camera:

• Switch off the autofocus.

• Set the distance to ∞.

• Set the automatic exposure control to time

priority.

• Set the aperture as wide as possible (i.e.

select a small f-stop number!).

Not all cameras feature these possibilities.
Please see the operating instructions of the
camera used.

• Loosen the set screw (3-15/3).

• Vary the distance between the camera lens

and the lens mount in steps, i.e. move the
sliding mount with camera on the lens
mount by defined steps.

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Operation

Axiostar plus Attaching adapters for digital compact cameras Carl Zeiss

• Zoom camera lens from wide-angle

position (W) through to tele position (T).

• Perform test until image is format-filling

without trimming or vignetting.

• Tighten the set screw (3-15/3) again.

No untrimmed image may be obtained when a
camera adapter combination is used which is
not recommended by Carl Zeiss.

3.11.2 Digital cameras with 52 mm

filter thread,
e.g. SONY DSC–S70, DSC-S75,
DSC-S85

When delivered, the sliding (3-16/4), the
threaded adapter ring M37/M52 (3-16/3) and
the lens mount (3-16/6) are premounted. The
drawing on the right shows the disassembled
status.

Furthermore, the threaded ring adapter
M37/52 (3-16/3) can be unscrewed from the
sliding mount (3-16/4) (not shown), i.e.
cameras with M37 can also be attached as
described in the previous chapter.

• Screw SONY VAD-S70 adapter ring

(3-16/2) into the objective/filter thread of
the DSC-S70 camera (3-16/1) until stop.

• Plug connector 60 (3-16/8) onto the

binocular phototube (3-16/9) and tighten
the 3 set screws (3-16/10).

• Screw the unit, consisting of sliding mount

(3-16/4), the threaded adapter ring
M37/M52 (3-16/3) and the lens mount
(3-16/6) into the SONY VAD-S70 adapter
ring (3-16/2).

• With the lens mount (3-16/6) pointing

forward, plug the unit onto connector 60
(3-16/8) until stop and tighten the set
screw (3-16/7).

1 SONY DSC-S70 camera
2 SONY VAD-S70 adapter ring
3 M37/M52 threaded adapter ring
4 Sliding mount
5 Set screw
6 Lens mount
7 Set screw
8 Connector 60 for microscope camera
9 Binocular phototube
10 Set screw

Fig. 3-16 Attaching Sony "Digital Still Camera DSC-

S70"

B 40-815 e 12/01 3-25

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Operation

Carl Zeiss Attaching adapters for digital compact cameras Axiostar plus

Depending on the microscope configuration or
camera used, the distance between the
camera lens and the lens mount (3-16/6) must
perhaps be optimized (see double arrow). This
is required in particular if an untrimmed image
cannot be achieved in any zoom position of
the camera lens.

Make the following settings on the camera:

• Switch off the autofocus.

• Set the distance to ∞.

• Set the automatic exposure control to time

priority.

• Set the aperture as wide as possible (i.e.

select a small f-stop number!).

Not all cameras feature these possibilities.
Please see the operating instructions of the
camera used.

• Loosen the set screw (3-16/5).

3.11.3 Attaching cameras with other

filter thread sizes

In principle, it is also possible to connect
cameras with a filter thread other than M37 or
M52. Suitable filter adapters or reduction rings
are available in photo shops. As mentioned
above, only a test can clarify whether such
cameras are compatible with the digital
adapters.

3.11.4 Sony DSC-S70, DC-S75, DSC-S85

on digital camera adapter 44
M52x0.75, 000000-1108-984

This adapter has been particularly designed for
the Sony DSC-S70. Combined with “Plan”
objectives, e.g. “Plan-Neofluar” objectives,
increased edge definition and lower distortion
can be achieved. Should future cameras prove
to be suitable, too, our sales staff will be
informed accordingly.

• Vary the distance between the camera lens

and the lens mount in steps, i.e. move the
sliding mount with camera on the lens
mount by defined steps.

• Zoom camera lens from wide-angle

position (W) through to tele position (T).

• Perform test until image is format-filling

without trimming or vignetting.

• Tighten the set screw (3-16/5) again.

No untrimmed image may be obtained when a
camera adapter combination is used which is
not recommended by Carl Zeiss.

Attachment of the camera requires the SONY
VAD S 70 adapter ring (Fig. 3-16/2). Further­more, the connecting piece 60-44 (456140­0000-000) must be inserted between the
camera adapter and the binocular tube.

Make the following settings on the camera:

• Switch off the autofocus.

• Set the distance to ∞.

• Set the automatic exposure control to time

priority.

• Set the aperture as wide as possible (i.e.

select a small f-stop number!).

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Operation

Axiostar plus Packing the mount Carl Zeiss

3.12 Axiostar plus - Packing the

mount

• Pull the binocular tube (3-17/6) completely

The portable case for the Axiostar plus can be
used to transport the basic Axiostar plus
equipment (stand with binocular tube 45° ICS,
Abbe condenser, mechanical stage, line cable,
dust cover).

• Before inserting the Axiostar plus into the

case, loosen the knurled screw on the stand
(3-17/9) by turning it by ¼, turn the
binocular tube 45°

ICS (3-17/6) around 180°

(eyepieces pointing backwards) and tighten
the knurled screw again.

• Open the four locks of the case and pull the

case cover (3-17/1) upwards to remove it

apart and slide the rubber foam component
(3-17/7) between the eyepiece tubes until
stop. It might be necessary to apply some
pressure on the rubber foam component
until the holding flaps (3-17/8) are flush
with the upper stand part.

• Line cable, dust cover and any small

accessories can be placed on the case floor
beside the stand and fixed in position using
the Velcro fasteners (3-17/3).

• Attach the case cover, close the locks with a

snap and, if required, lock them with the
enclosed key.

from the case floor (3-17/2).

• Insert microscope into the recess in the case

floor.

• Push rubber foam component (3-17/4) over

the condenser (3-17/5) and move the
condenser upwards until it contacts the
rubber foam component.

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Operation

Carl Zeiss Packing the mount Axiostar plus

1 Case cover
2 Case floor
3 Velcro fasteners
4 Rubber foam component
5 Condenser
6 Binocular tube 45°
7 Rubber foam component
8 Holding flaps
9Stand

Fig. 3-17 Inserting the Axiostar plus in the case

ICS

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Care, maintenance and trouble-shooting

Axiostar plus Contents, List of illustrations Carl Zeiss

CARE, MAINTENANCE AND

TROUBLESHOOTING

Contents

4 CARE, MAINTENANCE AND TROUBLE-SHOOTING.................................................. 4-2

4.1 Care and maintenance of the instrument.................................................................... 4-2

4.2 Troubleshooting ......................................................................................................... 4-3

4.2.1 Changing the fuses .................................................................................................... 4-3

4.2.2 Changing the 6 V, 20 W halogen lamp..................................................................... 4-4

4.2.3 Troubleshooting......................................................................................................... 4-5

4.2.4 Spares, wearing parts and tools .................................................................................. 4-8

4.3 Requesting service...................................................................................................... 4-9

List of illustrations

Fig. 4-1 Changing the fuses .................................................................................................... 4-3

Fig. 4-2 Changing the 6 V, 20 W halogen lamp....................................................................... 4-4

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Care, maintenance and trouble-shooting

Carl Zeiss Care and maintenance of the instrument Axiostar plus

4 CARE, MAINTENANCE AND

TROUBLE-SHOOTING

4.1 Care and maintenance of the

instrument

Care of the Axiostar plus is limited to the
following operations:

• Cover the instrument with the dust cover

after every use.

• Do not set up the instrument in a damp

room, i.e. max. humidity < 85 %.

• Cover open tubes with dust protection

caps.

• Remove dust and dirt from visible optical

surfaces using a brush, airblower, Q-tip,
optics cleaning paper or a cotton cloth.

• Remove water-soluble dirt (coffee, Coke,

etc.) after breathing on it and wiping it off
with a dust-free cotton cloth or a
moistened cloth. A mild cleaning agent can
also be added to the water.

• Remove stubborn, oily or greasy dirt

(immersion oils, fingerprints) with a wad of
cotton or a dust-free cotton cloth dipped in
the optics cleaning mixture L.
This cleaning mixture is produced of
90 Vol% benzoline and 10 Vol%
isopropanol (IPA). The various components
are also known under the following
synonyms:
benzoline: medical alcohol,

petroleum ether

Isopropanol 2-propanol,

dimethyl carbinol,
2-hydroxypropane

When using the Axiostar plus in humid climatic
zones, proceed as follows:

• Store the Axiostar plus in bright, dry and

well-ventilated rooms with a humidity of
less than 85 %; store particularly sensitive
components and accessories, such as
objectives and eyepieces, in a dry closet.

The risk of growth of fungus on opto­mechanical instruments always exists in the
following conditions:

− Relative humidity of more than 75% and

temperatures between +15 °C and +35 °C
for more than three days.

− Installation in dark rooms without air

ventilation.

− Dust deposits and fingerprints on optical

surfaces.

Clean the optical surface by moving in circles
starting in the middle. Slight pressure should be
exerted on the optics during cleaning.

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Care, maintenance and trouble-shooting

Axiostar plus Troubleshooting Carl Zeiss

4.2 Troubleshooting

Troubleshooting for the Axiostar plus is
described in further detail using the following
two examples:

− changing the fuses and

− changing the 6 V, 20 W halogen lamp

Further measures are summarized in the table
under4.2.2.

4.2.1 Changing the fuses

• Switch off the instrument via the on/off

switch (4-1/1), disconnect line plug from
the line and disconnect line connector from
instrument socket.

• Check line cable and plugs and exchange

them, if required.

• Use a 2.0 mm clockmaker‘s screwdriver to

press in the two spring shackles on the
sides of the fuse holder over the slot and
remove the fuse holder (4-1/3).

• Remove defective fuse inserts (4-1/2) from

the fuse holder and replace them with new
ones

• Reinsert the fuse holder containing the new

fuses; the spring shackles on the sides must
audibly lock into place.

T 0.8 A; 250 V; 5 × 20 mm fuse inserts must be
used.

For the catalog numbers of fuse inserts, please
see Section 4.2.4.

1 On/off switch
2 Fuse inserts
3 Fuse holder

Fig. 4-1 Changing the fuses

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Care, maintenance and trouble-shooting

Carl Zeiss Troubleshooting Axiostar plus

4.2.2 Changing the 6 V, 20 W

halogen lamp

Do not touch the lamp bulb with your bare
hands; if required, clean the bulb with clean
alcohol before switching it on for the first time
to prevent dirt from burning in.

The following procedure is required to
exchange the lamp:

1 Halogen lamp
2Cover

Fig. 4-2 Changing the 6 V, 20 W halogen lamp

• Switch off lamp supply via on/off switch

and allow lamp to cool down for approx.
15 minutes.

☞

☞

☞☞

When changing the lamp, it is not necessary to
remove the microscope from the ergonomic
base plate (in the event the base plate is being
used).

• Disconnect the earth-contact plug from the

line and remove the flat plug from the
instrument connector.

• Place the disconnected instrument on its

back to make the underside of the pyramid
stand accessible.

• Fold down cover (4-2/2) and pull out

defective halogen lamp (4-2/1).

• Use the protective cover to hold the new

6 V, 20 W halogen lamp and insert both
lamp pins carefully into the receptacles.

☞

The Axiostar plus microscope is supplied
with factory-aligned illumination. In general,
the illumination need not be adjusted even
when the lamp is exchanged by the
customer.

• Fold up cover again, return the stand to the

upright position and reconnect the stand to
the line.

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Care, maintenance and trouble-shooting

Axiostar plus Troubleshooting Carl Zeiss

4.2.3 Troubleshooting

Problem Cause Remedy

Vignetting or
inhomogeneous image
brightness in the field of
view;

the field of view is not
entirely visible

Low resolving power and
poor image contrast

The vis/doc knob on the
phototube is not in the correct
position (intermediate position)

Nosepiece with objective not
switched to stop position

Move the vis/doc knob on the
phototube to the correct position
(end position), see p. 3-18, 3-20

Switch nosepiece with objective
to stop position

Condenser not set correctly Set condenser correctly

(adjustment, centering),
see p. 3-4, 3-5

Aperture diaphragm not set
correctly

Luminous field diaphragm not set
correctly

Set aperture diaphragm correctly
(centering , opening), see p. 3-5

Set luminous field diaphragm
correctly (centering, opening), see
p. 3-5

Filter not inserted correctly in filter
mount

Aperture diaphragm opening not
set correctly

Insert filter correctly in filter
mount, see p. 3-3

Set aperture diaphragm opening
in accordance with the 2/3 rule
or depending on the specimen
features, see p. 3-5

Condenser not focused correctly Focus condenser, see p. 3-4, 3-5

Wrong cover slip thickness for

0.17 transmitted-light objectives

Use of no or unspecified
immersion oil with CZ immersion

Use the correct 0.17 mm cover
slips, see p. 1-7

Use CZ immersion oil 518 N, see
p. 4-8

objectives

Air bubbles in the immersion oil Remove air bubbles by applying

new oil or by moving objective
back and forth

Immersion oil at the front lens of a
dry objective

Corr. ring is not set to the correct
cover slip thickness

Dirt or dust on the optical surfaces
of objectives, eyepieces,

Clean the front lens of the dry
objective, see p. 4-2

Set the corr. ring to the correct
thickness, see p. 1-7

Clean the appropriate
components, see p. 4-2

condensers or filters

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Care, maintenance and trouble-shooting

Carl Zeiss Troubleshooting Axiostar plus

Problem Cause Remedy

Image aberration Condenser not set correctly Set condenser correctly,

see p. 3-3, 3-4, 3-5

Nosepiece not correctly switched
to stop position

Specimen is not fixed on the
mechanical stage

Great focus differences after
objective change

Left and right fields of view
cannot be combined into an

Focusing eyepieces are not set
correctly

Interpupillary distance of the
binocular tube is not set correctly

image

Focusing eyepieces are not set
correctly

Eye-fatiguing microscopy Interpupillary distance of the

binocular tube is not set correctly

Focusing eyepieces are not set
correctly

Image brightness not acceptable Reduce lamp voltage or insert

Correctly click-stop nosepiece

Correctly set specimen in
specimen holder and fix it

Set focusing eyepieces to the
appropriate ametropia, see p.
2-11 and 2-12

Set interpupillary distance
correctly, see p. 2-12

Set focusing eyepieces to the
appropriate ametropia,
see p. 2-11 and 2-12

Set correct interpupillary distance,
see p. 2-12

Set focusing eyepieces to the
appropriate ametropia,
see p. 2-11 and 2-12

conversion filter

Dirt or dust in the field of
view

Condenser not focused correctly Focus condenser,

see p. 3-3, 3-4, 3-5

Aperture diaphragm opening too
small

Set aperture diaphragm opening
in accordance with the 2/3 rule
or depending on the specimen
features, see p. 3-5

Dirt or dust on the optical surfaces
of objectives, eyepieces,
condensers, filters or specimens

Clean the optical surfaces of the
appropriate components,
see p. 4-2

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Care, maintenance and trouble-shooting

Axiostar plus Troubleshooting Carl Zeiss

Problem Cause Remedy

The 6 V, 20 W halogen
lamp does not function
although the on/off switch
is in “on“ position

The 6 V, 20 W halogen
lamp flickers, unstable
brightness

Line cable not connected to the
line

Connect line cable to the line and
make sure to check the
instrument and line voltage, see
p. 2-33

6 V, 20 W halogen lamp not
installed

Install 6 V, 20 W halogen lamp,
see p. 4-4

6 V, 20 W halogen lamp defective Exchange 6 V, 20 W halogen

lamp, see p. 4-4

The specified 6 V, 20 W halogen
lamp is not used

Use the specified 6 V, 20 W
halogen lamp,
see p. 4-8

Defective fuses Exchange fuses,

see p. 4-3

Electronics module possibly
defective

Have electronics module checked
by microscopy service and
replaced, if required, see p. 4-9

End of average life of 6 V, 20 W
halogen lamp

Incorrectly installed or broken line
cable

Replace 6 V, 20 W halogen lamp,
see p. 4-4

Correctly connect line cable or
replace it, see p. 2-33

The pins of the 6 V, 20 W halogen
lamp are not correctly inserted
into the receptacle

Correctly insert pins of
6 V, 20 halogen lamp into
receptacle, see p. 4-4

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Care, maintenance and trouble-shooting

Carl Zeiss Troubleshooting Axiostar plus

4.2.4 Spares, wearing parts and tools

Description Cat. No. Application

6 V, 20 W long-life halogen lamp 380079-9690-000 for the integrated illumination of the

Axiostar plus

SW 3 ball-headed screwdriver 000000-0069-551 for changing the phototube

SW 2 90°-offset hexagonal key for attaching colored-disk condenser and

swiveling polarizer

Eyepiece eyecup 444801-0000-000 recommended for techniques with a low

brightness level in order to suppress light
reflection

Dust cover for nosepiece
Dust cover for eyepiece tube

Immersol 518 N ;

20 ml oiler
100 ml bottle
250 ml bottle
500 ml bottle

Cleaning paper, 300 sheets 462975-0000-000 for cleaning optical surfaces

Fuse inserts (5 × 20 mm)
for all line voltages; T 0.8 A; 250 V 000000-0127-019

Light filters:
Interference wide-band filter ,green,
d = 32 × 4
Interference band filter, green 546,
d = 32 × 3

462981-0000-000
000000-0168-373

000000-1111-806
000000-1111-807
000000-1111-808
000000-1111-809

467803-0000-000

467807-0000-000

for covering unused instrument openings

for applications using immersion oil

electrical overload protection for the
integrated power supply

for enhancing the contrast in b/w
photography and phase contrast

CB 12 conversion filter, d = 32 × 2
CB 6 conversion filter, d = 32 × 2
CB 3 conversion filter, d = 32 × 2
3200-5500 K conversion filter, d = 32 × 2

N 0.25 neutral-density filter; d = 32 × 2
N 0.06 neutral-density filter; d = 32 × 2

White balance filter

0.50 gray filter, d = 32 × 4

0.12 gray filter, d = 32 × 4

0.03 gray filter, d = 32 × 4

KG 1 heat-protection filter, d = 32 × 2
Reflection heat protection filter, d = 32 × 2

Dust cover K
Dust cover G (only in combination with
binocular phototube)

467850-9901-000
467851-0000-000
467852-0000-000
467847-0000-000

467849-0000-000
467848-0000-000

000000-1155-789

467840-0000-000
467841-0000-000
467842-0000-000

467830-0000-000
467832-0000-000

459300-0000-000
459306-0000-000

for color photography using daylight
color films and artificial light color
reversal films

for visual observation and b/w
photography with transmission factor
information

for white light adjustment and contrast
enhancement for visual observation
for photography without color distortion,
with transmission factor information

for protecting sensitive specimens from
heat

for covering the instrument when it is not
used

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Care, maintenance and trouble-shooting

Axiostar plus Requesting service Carl Zeiss

4.3 Requesting service

All repairs of mechanical, optical or electronic
components inside the instrument and of the
electrical components of the Axiostar plus may
only be performed by Carl Zeiss service staff or
specially authorized personnel.

To ensure the optimum setting and trouble-free
function of your microscope over a longer
period of time, we would recommend that you
enter into a service/maintenance contract with
Carl Zeiss.

In the case of subsequent orders or when
service is required, please get in touch with
your local Carl Zeiss agency.

Within Germany, you can reach Carl Zeiss
Microscopy Service as follows:

Telephone: ++49 180 333 6 333

Fax: ++49 7364-20 4939

E-mail: [e-mail]

Further information is also available in the
Internet at the following address:

www.zeiss.de/micro

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Care, maintenance and trouble-shooting

Carl Zeiss Axiostar plus

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Axiostar plus Appendix Carl Zeiss

APPENDIX

Index......... .................................................................................................................................. A-2

List of Abbreviations ..................................................................................................................... A-4

Physical and Technical Units.......................................................................................................... A-5

Certification in Accordance with DIN EN ISO 9001 / DIN EN 46001
EC Conformity Declaration

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Appendix

Carl Zeiss Index Axiostar plus

Index

A

Abbe Condenser ....................2-14, 2-15, 2-16,

.....................................................3-4, 3-6, 3-7

Adapter................ 3-17, 3-18, 3-19, 3-21, 3-23

Ambient conditions ...................................1-11

Ametropia.................................................2-11

Analyzer....................................................3-12

Aperture diaphragm .............................3-4, 3-7

Aperture diaphragm lever...................3-6, 3-11

Application..................................................1-3

Attaching photomicrography equipment....3-18

B

Binocular phototube 30°/20.........................2-9

Binocular phototube 45°/20.........................2-8

Binocular Tube 30°/20 .................................2-9

Binocular Tube 45°/20 .................................2-8

Birefringence.............................................3-12

Brightfield ...................................................3-4

Brightness control.................................3-3, 3-4

C

Care..................................................... VIII, 4-2

Changing lamps .......................................... VIII

Colored disk carrier....................................2-20

Condenser.......................1-3, 1-13, 2-14, 2-15,

...................................... 3-3, 3-4, 3-5, 3-6, 3-7

Condenser carrier .....................1-3, 2-17, 2-14,

......................................2-15, 2-16, 2-18, 2-20

Connecting the instrument to the line........2-33

Conversion filter........................2-21, 3-3, 3-19

Cover slip thickness................................1-7, 1-8

D

Dark field slider ...........................................3-9

Darkfield condenser...................2-18, 2-19, 3-9

Digital compact cameras............................3-23

Dimensions................................................1-11

Diopter.................................................3-6, 3-7

Disconnect .............................................VII, VIII

Disposal........................................................ VII

Double tube ..............................................3-17

Dust cover........................................3-19, 3-20

Dust-protection glass ...................................3-3

E

EMC.............................................................. VI

Epi-fluorescence ........................................3-14

Epi-fluorescence illumination.............2-22, 3-14

Ergonomic base plate...... 2-3, 2-4, 2-6, 2-7, 2-9

Exchange the lamp ......................................4-4

Eyepiece.................................. 1-8, 2-10, 2-12,

......................................... 3-5, 3-7, 3-16, 3-17

Eyepiece reticle................ 1-9, 2-11, 3-16, 3-18

F

Field number .............................................1-13

Filter...................................................3-3, 3-19

Filter clamp................................................2-21

Filter stop carrier......................2-23, 2-27, 3-15

Fluorescence.....................................2-22, 3-14

Fluorescent Lamp..............................1-12, 2-24

Fuses ...........................................VII, 1-11, 4-3

H

Halogen lamp............. 1-3, 1-12, 2-33, 3-3, 4-3

I

Immersion objectiv.......................................1-7

Instrument description .................................1-3

Instrument Safety ..........................................VI

Interference wide-band filter........................3-3

Interpupillary distance................................1-13

K

Knurled ring ................................................3-5

KÖHLER illumination principle ...............3-4, 3-9

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Appendix

Axiostar plus Index Carl Zeiss

L

LED illuminator ............................................3-3

Line voltage......................................1-11, 2-33

Luminous-field diaphragm ...................3-3, 3-4,

.....................................................3-5, 3-7, 3-9

M

Magnification..............................................1-7

Magnification numerical aperture ................1-8

Maintenance ........................................ VIII, 4-2

Measurement of lengths............................3-16

Mechanical stage................................2-13, 3-4

Microscope camera ...................................3-20

N

Nosepiece ............................................1-3, 3-9

Numeric aperture ..........................1-7, 3-4, 3-6

O

S

Service ........................................................4-9

Slider for darkfield.......................................3-8

Slider for phase contrast ..............2-14, 3-4, 3-6

Slider for phase darkfield ...........................2-14

SLR camera................................................3-18

Spares .........................................................4-8

Stage micrometers.......................................1-9

Start-Up ................................................ VII, 2-3

Storage .....................................................1-11

System overview..........................................1-4

T

Tools...........................................................4-8

Transmitted-light brightfield.........................3-4

Transmitted-light darkfield....................3-8, 3-9

Transmitted-light polarization contrast .......3-12

Transport............................................. VII, 1-11

Troubleshooting ..........................................4-3

Objective........ 1-7, 1-8, 1-13, 2-10, 2-16, 2-19,

............................. 2-21, 3-4, 3-5, 3-6, 3-7, 3-8

Objective magnification ...............................1-7

On/off switch ..............................1-3, 2-33, 3-3

Operation.............................................. VII, 3-3

Opto-mechanical data ............................... 1-13

P

P&C reflector module ................................2-30

Packing ..................................................... 3-27

Phase contrast.............................................3-7

Phase contrast objective...............................3-6

Polarizer....................................................3-12

Power unit ................................................3-17

U

Unpacking ............................................VII, 2-3

V

Video adapter............................................3-21

Viewing angle............................................1-13

Viewing height .................. 1-3, 1-13, 2-6, 2-12

W

Warranty..................................................... VIII

Wearing parts..............................................4-8

Weight......................................................1-11

White-balance filter ...................................2-21

Wide-angle unit.........................................2-20

B 40-815 e 12/01 A-3

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Appendix

Carl Zeiss Abbreviations Axiostar plus

List of Abbreviations

A-Plan Achromatic objectives featuring improved image flatness (ICS line)
Br. suitable for eyeglass wearers
CB Correction Blue (conversion filter)

CCD Charge C

oupled Device
CE EC conformity declaration
CP-Achromat Achromatic objective (ICS line)
CSA Canadian S

tandards Association

d diameter
D dark field or cover slip thickness
DIN Deutsches Institut für Normung (German standards association)
doc documentation
DX coding system for the storage of electronically legible information, e. g. film speed

EG European Community
EMV electromagnetic compatibility
EN European standards
ENG Electronic N

ews Gathering
E-PL name of eyepiece type with aspheric lens and flat field of view
EWG European Economic Community

FISH Fluorescence In Situ Hybridization
foc. Focusing
H bright field

HAL halogen lamp
HBO mercury vapor short-arc lamp

ICS Infinity C

olor corrected System
IEC International Electrotechnical Commission
IP International Protection (protection type)
ISO International Standard Organization

MC Microscope C

amera

N neutral-density filter
N.A. numerical aperture

Ph phase contrast
PL plan

R right (control on the right of the mechanical stage)

A-4 B 40-815 e 12/01

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Appendix

Axiostar plus Physical and Technical Units Carl Zeiss

SLR Single L

ens Reflex
SW wrench opening
s/w black-and-white

T slow-blow (fuse type)
T2-Adapter standard adapter for 35 mm cameras

V

Obj

magnification of the objective
VDE association of German electrotechnicians
vis visual

W 0,8 Whitworth-type thread (inch thread) 0.8

Physical and Technical Units

A ampere

° angular degree
°C Centigrade

h hour
hPa hectopascal
Hz Hertz

K Kelvin
kg kilogram

lm Lumen (light flux)

mm millimeter
µm micrometer

VVolt

W Watt

“ inch

B 40-815 e 12/01 A-5

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