8.2Cleaning the infrared filter or protective glass ............................................... 21
9 Disposal and Recycling ......................................................................................... 22
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1 General information | 1.1 IntroductionZEISS
1 General information
1.1 Introduction
Welcome
Content
Welcome to the Axiocam 512 user documentation.
The camera is a professional digital camera for universal light microscopy with a high resolution
12 Megapixel sensor and a USB 3.0 interface.
To set up the camera correctly, follow the instructions in this guide step by step.
ChapterContent
About this guideIncludes an introduction and overview about this guide.
SafetyProvides important information on a safe handling with the
Axiocam 512.
Read this chapter, before unpacking the camera and
putting it into operation.
ShipmentDescribes the contents of delivery and optional attach-
ments.
Technical dataIncludes all technical data to your camera.
Connecting the cameraProvides detailed instructions on connecting and using the
camera.
Installing software and driversDescribes how to install ZEISS software and camera drivers.
Trouble-shootingIncludes some solutions to various problems.
If you can not solve your problem, contact the ZEISS support.
MaintenanceDescribes some measures for the maintenance and care of
your camera. For greater damage always contact the ZEISS
support.
Disposal and RecyclingIncludes important instructions for disposal and recycling.
1.2 Explanation warning messages and additional information
The safety notes in this document follow a system of risk levels, defined in the following manner:
CAUTION
Risk of personal injury
CAUTION indicates a potentially hazardous situation which, if not avoided, may result in minor
or moderate personal injury.
NOTICE
Risk of property damage
NOTICE indicates a property damage message. In addition, NOTICE is used for data loss or corrupt data.
User Guide ZEISS Axiocam 512 color/mono | en-US | Rev.15
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1 General information | 1.3 Text formats and conventionsZEISS
Info
Indicates useful additional information. It helps you to make your daily work easier, but it is optional. There is no risk for personal injury or property damage involved.
1.3 Text formats and conventions
Bold texts
Bold is used for texts within the software like names of GUI elements (e.g. buttons, sections,
tools, menus), buttons on a device, and product names (e.g. MTB 2011).
Font type "Courier"
Used for programming code, e.g. macro code as well as for anything that you would type literally when programming, including keywords, data types, constants, method names, variables,
class names, and interface names.
Prerequisite
Shortcuts and key commands
Shortcuts are written like Crtl+C, meaning you should press Crtl-Key and C-Key simultaneously.
Procedures
The following formats are used for procedures (instructive sequences):
ü
stands for a condition which must be fulfilled before starting with the action.
1. stands for a single step the user is asked to perform.
Web-Links
Web links appear in blue text. To open the linked website, simply click on the link. Please make
sure you have an internet connection established before opening the web link.
User Guide ZEISS Axiocam 512 color/mono | en-US | Rev.1
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2 Safety | 2.1 Safety notesZEISS
2 Safety
2.1 Safety notes
The camera has been manufactured and tested by ZEISS according to the regulations specified in
CE and has left the manufacturer’s premises in perfect working order. In order to ensure that this
condition is maintained and to avoid any risks when operating the system, the user must comply
with any notes and warnings contained in this manual. The manufacturer shall be exempt from
statutory liability for accidents should the operator fail to observe the safety regulations.
CAUTION
Personal Injury
To avoid personal injury, read and adhere to the safety notes below.
4 To avoid the risk of fire or explosion, do not use the camera near inflammable liquids or
gases.
4 Setup, expansions, re-adjustments, alterations, and repairs must be carried out only by
persons who have been authorized by ZEISS.
4 Do not allow any cables, particularly power cords, to trail across the floor, where they can
be snagged by people walking past.
4 Protect the cables from excessive heat (e.g. halogen lamps, microscope fluorescence illu-
mination).
NOTICE
To avoid equipment damage, data loss, or corrupted data, read and adhere to the safety notes
below.
4 Protect the camera against mechanical impact. External damage may affect the operation
of inner components.
4 Keep chemicals and fluids away from the camera.
4 Make sure there is sufficient ventilation of the camera head. Avoid direct exposure to sun-
light and locations near heat sources (radiators, stoves). Overheating can cause noisy images.
4 Use the camera in a clean and dry location.
4 Attach all connectors firmly and securely.
4 Use only the accessories supplied by ZEISS, when applicable.
4 Use only normal microscope cleaning material to clean the camera housing.
4 Contact your local ZEISS service organization if a repair is necessary.
4 Save all your data, such as images, measurement data, archives, reports, forms and docu-
ments, at regular intervals on an external storage medium. Otherwise it cannot be avoided
that access to this data may be lost as a result of operational errors or hardware defects.
ZEISS accepts no liability for consequential damage resulting from insufficient data protection.
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2 Safety | 2.2 Limitation of liabilityZEISS
2.2 Limitation of liability
No warranty shall be assumed by ZEISS during the warranty period if the equipment is operated
without observing the safety regulations. In any such case, ZEISS shall be exempt from statutory liability for accidents resulting from such operation.
2.3 Warranty
ZEISS shall be exempt from any warranty obligations should the user fail to observe the safety regulations. ZEISS only guarantees the safety, reliability, and performance of the system if the safety
notes are closely observed.
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3 Technical Data | 3.1 Axiocam 512 color/monoZEISS
3 Technical Data
3.1 Axiocam 512 color/mono
FeatureValue
Sensor ModelAxiocam 512 color:
Sony ICX 834AQG, EXviewHAD CCD II ™, Progressive
Scan, Quad-Port Readout, pre-selected sensor quality
Max Full Well Capacity (typical)9.000 e- per pixel
Signal AmplificationAdjustable analog amplification 1x, 2x, 3x
Digitization14 Bit / Pixel
Read Out Speed39 MHz, 13 Mhz, switchable readout clock speed
Readout Noise
(typical)
Dynamic Range (typical)1:1380 (63dB)
Dark Current (typical)< 0,1 e-/p/s at 23 °C sensor temperature
CoolingRegulated Peltier-Cooling,
6.8 e- at 39 Mhz
6.5 e- at 13 Mhz
(power supplied through USB 3.0 and USB 2.0 ports),
Delta-T 20° C, sensor temperature 23°C
Dark Current CompensationDigital Dark Current Compensation for optimum low
light performance at long exposure times,
Automatic Hot Pixel Correction
Exposure Time Range250 µs to 60 s
Color Interpolation ModesAxiocam 512 color:
“High Speed”: optimum speed color interpolation
“High Quality”: optimum quality color interpolation
Data-Post Processing (optional)§ Objective specific shading correction
§ Sharpening, Noise Filter, Color Enhancement
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3 Technical Data | 3.1 Axiocam 512 color/monoZEISS
FeatureValue
§ Black Reference, Dark Current Compensation
Special Features§ Time Stamp from Camera for precise acquisition
time point
§ Auto Switch Mode for Single Port/Dual Port/Quad
Port Readout
§ Adjustable intensity of status LED
Special Preset ModesEight pre-loadable sets of imaging parameters for
speed-optimized multi-modal image acquisition,
overlapping exposure and readout for fast time lapse
imaging
Switchable Sensor Output AmplifierSingle Port Readout mode for long exposure times for
maximum signal quality, Dual Port and Quad Port
Readout Mode for improved readout speed at full resolution,
automatic port activation mode or full manual mode
Region of Interest (ROI)User defined imaging sub area for improvement of
readout speed and reduction of amount of data
Hardware TriggerGalvanic isolated I/O signals,
three output signals: exposure time, readout time, trigger ready (i.e. for controlling external mechanical shutters),
one trigger input for exposure control, 5V auxiliary
voltage, GND
Status LEDTop LED: Camera Status (acquisition, power, cooling,
max. 80% relative humidity, non-condensing,
free air circulation required,
Ambient Conditions (Storage)-15° ... +60° Celsius
90% relative humidity at +40° Celsius,
80% relative at +20° Celsius, non-condensing
3.1.1 Binning Mode and Frame Rate
Axiocam 512 color
Axiocam 512 mono
BinningH x VModeFPS@1ms
1x14248 x 2832Color/Mono10
2x22120 x 1416Mono19
3x31416 x 944Color/Mono26
4x41056 x 708Mono31
5x5848 x 564Color/Mono35
ROI1936 x 1080Color/Mono22
ROI1936 x 512Color/Mono36
BinningH x VModeFPS@1ms
1x14248 x 2832Mono11
2x22120 x 1416Mono20
3x31416 x 944Mono27
4x41056 x 708Mono32
5x5848 x 564Mono36
ROI1936 x 1080Mono23
ROI1936 x 512Mono36
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3 Technical Data | 3.1 Axiocam 512 color/monoZEISS
3.1.2 Live Image Frame Rates
Axiocam 512 color
Axiocam 512 mono
Max. Ratings at optimum hardware settings, Color Enhancement off:
Max. Frame RateBinning Factor / ModePixel Count
10 frames/s1 / slow4248 x 2832
26 frames/s3 / medium1416 x 944
35 frames/s5 / fast848 x 564
Max. Ratings at optimum hardware settings:
Max. Frame RateBinning Factor / ModePixel Count
10 frames/s1 / slow4248 x 2832
19 frames/s2 / medium2120x1416
26 frames/s3 / fast1416x944
Info
Computer hardware, operating system and software may decrease the frame rates. Selecting a
part of the sensor area or applying binning can increase the frame rate. All specifications are
subject to change without notice.
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3 Technical Data | 3.1 Axiocam 512 color/monoZEISS
3.1.3 Spectral Sensitivity
Axiocam 512 color
Axiocam 512 mono
Fig.1: Axiocam 512 color incl. Hoya C5000 IR Cut Filter
Fig.2: Spectral Sensitivity Axiocam 512 mono
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4 ShipmentZEISS
4 Shipment
ContentOrder Number
1 x Axiocam 512 color camera or
1 x Axiocam 512 mono camera
1 x USB cable (2 in one) for power supply (USB 2.0 = black cable)
and data transfer (USB 3.0 = blue cable)
1 x PCI express interface card ( 4 x USB 3.0)
1 x 15 Pin SATA power connector cable
1 x DVD with device driver for ZEISS software as well as this
guide as a PDF file.
Accessory (optional)
426560-9000-000
426560-9020-000
Fig.3: Axiocam trigger cable
ContentOrder Number
Trigger cable426557-0001-000
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5 Connecting the Camera | 5.1 Camera OverviewZEISS
5 Connecting the Camera
5.1 Camera Overview
Camera connections
Fig.4: Camera back
NumberDescription
1Trigger connection
2USB 2.0 connection for power supply and cooling
3USB 3.0 connection for image / controller data and power supply
4¼ “ photo thread (tripod connection)
5.2 Building in the interface card
This chapter describes how to build in the PCI express interface card (4 x USB 3.0) into your PC.
NOTICE
Static Electricity
Static electricity can damage electronic components. To protect electronic components against
static electricity, do not touch them until you have grounded yourself to the casing of the device. Never touch the contacts of the electronic components. We also recommend that you
work only on an antistatic mat.
Prerequisite
User Guide ZEISS Axiocam 512 color/mono | en-US | Rev.115
ü
You have read the documentation of the interface card and of your computer before inserting
the interface card.
1. Switch off your PC and all connected peripherals.
2. Disconnect the PC and the peripherals from the mains and open the computer case.
3. Examine your PC’s power supply to see if it has Molex type connectors or SATA type connectors. Choose the appropriate power connector cable for the following steps. If your PC’s
power supply has both connector types, choose only one cable.
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5 Connecting the Camera | 5.3 Mounting the camera on the microsopeZEISS
4. Connect the appropriate power connector cable to one of the open connectors from your
computer’s power supply.
5. Connect the other end of the power cable to the PCIe interface card.
6. Insert the PCIe interface card into the appropriate slot on your computer.
7. Close the computer case and reconnect the peripherals.
You can now mount the camera on the microscope and connect it to the PC via the USB 3.0 interface, see chapter Connecting the camera to the PC [}16].
5.3 Mounting the camera on the microsope
To mount the camera onto your microscope’s TV port, use a C-mount adapter. The adapter is not
included in the shipment. You will find some suitbale examples for adapters in the list below:
PortNameOrder Number
44Video Adapter 44 C 2/3" 1,0x452995-0000-000
60Camera Adapter 60 C 1" 1,0x456105-9901-000
60Video Adapter 60 C 2/3" 0,63x000000-1069-414
60NCamera Adapter 60N-C 1" 1,0x426114-0000-000
60NCamera Adapter 60N-C 2/3" 0,63x426113-0000-000
NOTICE
Loss of warranty
The Axiocam 512 color is delivered with an integrated IR filter (infrared cut filter). The Axiocam
512 mono is delivered with an integrated BK7 protective glas. This will protect the camera
against dust and reduces optical interferences.
4 The IR filter is situated approx. 5 mm behind the outer edge of the C-mount opening in
the camera.
4 Due to this pre-mounted IR barrier filter, C-mount adapters that screw more than 5 mm
into the thread cannot be screwed into the camera.
4 Do not remove the filter or the protective glas otherwise the warranty will be lost.
1. Remove the dust cap from the camera’s C-mount port.
2. Screw the adapter in as far as it will go.
3. Mount the camera onto the microscope’s TV port. Ensure that no dust enters the opening
of the camera or the microscope’s TV port.
5.4 Connecting the camera to the PC
1. Lay the USB cable (2 in 1) carefully between the camera and the PC.
2. Connect the USB cables to the USB 3.0 interface card of the PC.
3. Connect the USB cables with the camera.
4. If you switch on the PC, the camera will be also switched on.
Power is supplied via the USB 3.0 cable. The USB 2.0 cable supplies the peletier cooling. By
switching off the PC, the camera will be switched of as well. If the camera is installed correctly, the status LED will be red. If you start the software the LED will turn blue. You`ll find
a detailed description of the LED status light in the chapter Function indicator [
User Guide ZEISS Axiocam 512 color/mono | en-US | Rev.1
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18].
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5 Connecting the Camera | 5.5 Connecting the trigger cableZEISS
5.5 Connecting the trigger cable
In this chapter you will learn how to connect the trigger cable (optional) to the camera and the
SVB (signal distribution box).
Fig.5: Trigger cable
NumberDescription
1Trigger (Trigger-In)
2GPO 0 (Trigger Out, for exposure time incl. predelay, coresponding to
shutter control
3GPO 1 (Trigger Ready, for Readout Time data)
4GPO 2
5GPOPWR
6AUXPWR
7Commercial Micro-D
The numbers in brackets in the guide below refer to the connectors shown in the illustration
"Trigger cable" and described in the table below it.
1. Plug the connector Commercial Micro D (7) in the trigger connector on the back of the
camera, see Camera Overview [}15].
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5 Connecting the Camera | 5.6 Function indicatorZEISS
2. Plug the connector Trigger (1) in the socket
Trigger Out of the SVB (Signal distribution
box).
Fig.6: Signal distribution box (SVB) backside
3. Plug the connector GPO 0 (2) in the socket Trigger In 1 of the SVB (Signal distribution
box).
4. Plug the connector GPO 1 (3) in the socket Trigger In 2 of the SVB.
5. Connect the connectors GPOPWR (5) and AUXPWR (6) of the trigger cable together.
à
GPO 2 (4) is not connected.
5.6 Function indicator
Signal colorDescription
BlueUSB 3.0 connection (Camera) connected with USB 3.0 connection (PC)
GreenUSB 3.0 connection (Camera) connected with USB 2.0 connection (PC)
VioletUSB 3.0 connection (Camera) connected with USB 3.0 connection (PC)
YellowUSB 3.0 connection (Camera) connected with USB 2.0 connection (PC)
RedPower supply attached, no driver loaded, camera not yet initialized
No lightNo power supply or software shut down (camera in power down
Power supply (USB 2.0 cable) attached
Recommended configuration for best camera performance
Power supply (USB 2.0 cable) attached
Power supply (USB 2.0 cable) not attached
Power supply (USB 2.0 cable) not attached
mode)
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6 Installing software and driversZEISS
6 Installing software and drivers
Prerequisite
ü
To acquire images with the Axiocam 512 on a PC, you must install ZEISS software (e.g. ZEN or
AxioVision). You will find the software installation on the DVD delivered. The camera drivers
will be installed automatically during the installation of the software.
ü
Before starting the installation of the software and drivers, the camera must be connected to
the PC.
1. Install the software according to the installation guide delivered with the software.
2. During the installation you will be asked to install the camera drivers. Follow the procedure
by confirming the upcoming messages.
3. Restart your PC after the installation.
4. You can check in the device manager if the driver installation was successful.
You have installed the software and the camera drivers. You can now start acquiring images with
your camera using ZEISS software. For more information, please read the software documentation.
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7 Troubleshooting | 7.1 SoftwareZEISS
7 Troubleshooting
7.1 Software
7.1.1 The camera does not appear in the menu of selectable cameras
§Make sure that you have connected the camera and installed the software and drivers in ac-
cordance to the instructions in this manual.
§Make sure that you installed the software and drivers with administrative rights. Install the
driver with administrative rights again if necessary.
7.1.2 You don't see a camera image on your screen
§Check the light path setting of the microscope.
§Is the status LED on the camera on? If not, check the cable connections between camera and
computer.
§Execute a reset of the camera.
§Execute an automatic exposure measurement.
§Check the display adjustments for the live image.
§Check the aperture diaphragm of the microscope.
§Check the position of the beam splitter between the ocular and the TV port.
7.1.3 The color of my image does not correspond to the impression through the
ocular
In case of color cameras:
§Check white balance and, if necessary, repeat white balance.
§Check the monitor’s color temperature setting. If necessary, reduce this to the lowest value
that can be set (usually 5200 K).
In case of monochrome cameras:
§Images of monochrome cameras are displayed by using overlay colors to represent the colors
of fluorescence dyes on the monitor. If the color is different to the visual impression in the
eyepiece, select a different overlay color.
7.2 Hardware
7.2.1 Vibrations
Sudden jolt, vibration or moving objects during the acquisition can detract from the image quality.
The light intensity during exposure can change as well.
§To avoid jolts, use damping systems on your microscope.
§Repeat the acquisition and take care to ensure the conditions remain calm.
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8 Maintenance | 8.1 Optical SystemZEISS
8 Maintenance
8.1 Optical System
The internal optical components of the camera should always be protected. If no lens, or TV
adapter with optics, is screwed into the camera's C-Mount thread, the camera's sensor and protective glass must be protected by screwing the protective cap onto the camera's C-Mount
thread.
8.2 Cleaning the infrared filter or protective glass
Contamination of the IR filter (for color cameras only) or the protective glass has an adverse effect
on the quality of the resulting image (dark points, cloudy structures in the image). If there is dry
dust on the front side of the infrared filter or protective glass, you can clean it with a soft brush or
with cotton (wool). Use cleaning fluid for optics/lenses only to clean the IR filter. Do not use tap
water to clean the IR filter.
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9 Disposal and RecyclingZEISS
9 Disposal and Recycling
This product has been developed, tested and manufactured in accordance with the applicable environmental provisions and directives of the European Union:
§The product and its accessories comply with EU directives 2002/95/EC (RoHS) and 2002/96/EC
(WEEE), insofar as these apply to this product.
§ZEISS has implemented a take-back and recycling process that ensures that proper recycling is
carried out in accordance with the aforementioned EU directives.
§Please contact your ZEISS sales/service organization for details relating to disposal and recy-
cling.
§This product must not be disposed of with domestic waste or using municipal waste disposal
services. In the event of resale, the seller must inform the buyer of the need to dispose of the
product appropriately.
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Carl Zeiss Microscopy GmbH
Carl-Zeiss-Promenade 10
07745 Jena
Germany