YSI 9600 User Manual
YSI Environmental
Pure Data for a Healthy Planet.™
YSI 9600
Nitrate Monitor
Operations
Manual
NITRATE MONITIORING WITH YSI 9600 AT
FRESHWATER SITE; 1 HOUR SAMPLE INTERVAL
6
5
4
3
mg/L NO3-N
2
1
0
7/10/03 0:00 7/15/03 0:00 7/20/03 0:00 7/25/03 0:00 7/30/03 0:00
Time
9600
USER QC
YSI QC
YSI Environmental 2 Model 9600 Nitrate Monitor
TABLE OF CONTENTS
SECTION 1 INTRODUCTION TO THE 9600 7
SECTION 2 GETTING STARTED 8
2.1 UNPACKING 8
2.2 UNDERSTANDING THE 9600 MODE OF OPERATION 12
2.3 PREPARING REAGENTS 13
2.4 FILLING THE REAGENT BAGS 16
2.5 PREPARING THE CADMIUM COLUMN 18
2.6 INSTALLING THE CADMIUM COLUMN 20
2.7 INSTALLING THE REAGENT BAGS 22
2.8 USING THE WASTE BAGS 23
2.9 ASSEMBLING THE NITRATE MONITOR 25
SECTION 3 PREPARING FOR A 9600 DEPLOYMENT 27
3.1 INSTALLING THE NUviewTM PC SOFTWARE 27
3.2 CHOOSING A CABLE FOR THE 9600 28
3.3 POWERING THE 9600 29
3.4 ATTACHING THE SECOND STANDARD BAG 31
3.5 INTERFACING WITH THE 9600 32
SECTION 4 STARTING A 9600 DEPLOYMENT 34
4.1 UNDERSTANDING CALIBRATION OF THE 9600 34
4.2 CHECK LIST FOR DEPLOYMENT READINESS 34
4.3 PREDEPLOYMENT SYSTEM CHECK 36
4.4 PREDEPLOYMENT SET-UP 39
4.5 INSTALLING THE INLET FILTER 42
SECTION 5 USING THE 9600 IN THE FIELD 44
5.1 PICKING A DEPLOYMENT SITE 44
5.2 POWERING THE 9600 DURING A DEPLOYMENT 44
5.3 VIEWING AND UPLOADING DATA DURING A DEPLOYMENT 45
5.4 STOPPING A DEPLOYMENT 49
5.5 DEPLOYING THE 9600 WITH A DATA COLLECTION PLATFORM 49
5.6 9600 POSTDEPLOYMENT TEST – QUALITY ASSURANCE 56
5.7 9600 MAINTENANCE AND NEW DEPLOYMENTS 56
SECTION 6 MANAGING 9600 FILES 58
6.1 INTRODUCTION 58
6.2 DIRECTORY 58
6.3 UPLOAD FILES TO YOUR PC 59
6.4 DELETING FILES 59
6.5 DETERMINING AVAILABLE FILE MEMORY 60
SECTION 7 ANALYZING DATA WITH ECOWATCH FOR WINDOWS 61
7.1 INTRODUCTION 61
7.2 INSTALLING ECOWATCH FOR WINDOWS 61
7.3 GLOSSARY 61
7.4 VIEWING AND PLOTTING NITRATE DATA 63
7.5 USING TOOLBAR SHORTCUTS 68
YSI Environmental 3 Model 9600 Nitrate Monitor
SECTION 8 PRINCIPLES OF OPERATION 70
8.1 CHEMISTRY 70
8.2 EFFECT OF PH 71
8.3 FLOW SYSTEM DETAILS 71
8.4 PUMPING SEQUENCES 72
8.5 FLOW RESTRICTION PROBLEMS 72
8.6 CALIBRATION OF SAMPLE AN D STANDARD PUMPS 72
8.7 CALIBRATION DETAILS 72
8.8 SYSTEM LINEARITY 73
8.9 TEMPERATURE EFFECTS 74
SECTION 9 UPGRADING 9600 FIRMWARE AND SOFTWARE 76
9.1 UPGRADING 9600 MONITOR FIRMWARE 76
9.2 UPGRADING NUVIEW PC SOFTWARE 77
SECTION 10 TROUBLESHOOTING 78
SECTION 11 FERRITE BEAD INSTALLATION 80
SECTION 12 9600 SAFETY CONSIDERATIONS 81
SECTION 13 WARRANTY AND SERVICE INFORMATION 82
SECTION 14 REQUIRED NOTICE 85
SECTION 15 9600 SPECIFICATIONS 86
15.1 SENSORS 86
15.2 SYSTEM 86
SECTION 16 ACCESSORIES 87
SECTION 17 FREQUENTLY ASKED QUESTIONS 88
APPENDIX A ECOWATCH FOR WINDOWS COMMANDS 96
YSI Environmental 4 Model 9600 Nitrate Monitor
SAFETY AND EMC CONSIDERATIONS
9600 NITRATE MONITOR
Before using the 9600, the user should carefully read the caution messages listed below and should
review Sections 11 and 12 of this manual on Safety and EMC considerations.
CAUTION: Operation of the YSI 9600 Nitrate Monitor requires the use of a variety of
chemicals. With one exception (elemental cadmium), the user is required to purchase these
chemicals from a vendor of their choice. Users should consult the Material Safety Data Sheets
(MSDS) supplied by these vendors for all reagents before their use. Users should ALWAYS wear
appropriate personal protective equipment including gloves and safety glasses with side shields when
handling any of these chemicals.
For proper operation of the YSI 9600, cadmium metal MUST be purchased in packaged form as the
YSI Model 9612 Cadmium Column Assembly. An MSDS for the material captured in the cadmium
column is automatically supplied to the user by YSI. To protect against potential hazards
associated with inhalation of cadmium dust, the cadmium column should be handled in a fume hood
during activation. After the reducing capacity of the cadmium column has been exhausted, the entire
sealed column should be disposed of in accordance with regulatory requirements, e.g. hazardous
waste. Users should NEVER open the cadmium column in attempts at regeneration. If the
cadmium is accidentally spilled, appropriate spill measures should be taken.
The waste generated from use of the YSI 9600 contains potentially toxic organic chemicals, but most
importantly, contains Cd
Users should NEVER discharge waste from the YSI 9600 into the environment, instead retaining it in
the waste bags that are provided with the instrument. Likewise, unless specifically approved by the
local wastewater authority, users should NEVER dispose of the reaction waste into a sanitary or
storm sewer. The waste should be disposed of in accordance with regulatory requirements with an
approved waste handler.
2+
that according to the USEPA is toxic and a probable human carcinogen.
CAUTION FOR INSTRUMENTS USE D IN EUROPEAN CO M MUNITY (CE)
COUNTRIES:
Nitrate Analyzer complies with the Residential, Commercial and Light Industrial Class B Limits for
radio-frequency
laboratory equipment with no qualifiers. However, if you are using your YSI 9600 Nitrate Monitor in a
European Community (CE) country or in Australia or New Zealand, when communicating with the
instrument you must attach a ferrite bead to the interface cable in order to comply with the industrial
immunity specified in EN61326 for Industrial, Scientific and Medical laboratory equipment. The
ferrite bead (YSI Item Number 699296) is NOT supplied as standard equipment with the 9600 and
should be purchased as an accessory by customers requiring its use. Attachment of the bead is shown
graphically in Section 11.
YSI Environmental 5 Model 9600 Nitrate Monitor
As described in the Declaration of Conformity (YSI Item # 699298), the YSI 9600
emissions specified in EN55011 (CISPR11) for Industrial, Scientific and Medical
YSI Environmental 6 Model 9600 Nitrate Monitor
SECTION 1 INTRODUCTION TO THE 9600
The YSI 9600 Nitrate Monitor is a powerful instrument that allows the user to continuously record nitrate
levels at a variable sample interval and thus provides a much greater capability to detect nutrient events
than the spot sampling activities which have historically been carried out. The 9600 can be custom
configured with regard to detector cell to provide maximum accuracy for the type of water that you
normally monitor. For applications where typical nitrate levels are over 2 mg/L of nitrate-nitrogen, the
9600 should be configured with a 2 mm cell (designated 9600-02 for ordering purposes). For applications
where typical nitrate levels are below 2 mg/L, the monitor should be equipped with a 10 mm detector cell
and designated 9600-10. The 9600-02 and 9600-10 are designed primarily for freshwater and
estuarine/near coastal applications, respectively, but there may be some cases where the greater range of the
9600-02 will be required for brackish water studies and some cases where freshwater nitrate levels are
consistently low enough to require the greater accuracy of the 9600-10. Note that users cannot change the
detector cell that was purchased with their particular 9600 in order to meet the requirement of a new
application. While detector cells can be switched from 2 mm to 10 mm or vice versa, this action must be
carried out by factory-trained personnel.
To accurately measure nitrate, the 9600 uses flow injection technology combined with a chemical reaction
sequence which is defined in literature from both the USEPA and Standard Methods for the Examination of
Water and Wastewater. The intensity of the color produced by the chemical reaction sequence is
determined by standard absorbance spectroscopy and compared to that of a standard of known
concentration in order to quantify the amount of nitrate in an environmental sample. The use of flow
injection technology minimizes the amount of reagents required and the amount of waste generated.
Key features of the YSI 9600:
• Works on standard cadmium reduction/diazotization principle. Standard Methods #4500-NO
• Can be custom configured relative to detector cell for most freshwater applications (2 mm) or for most
estuarine and near coastal applications (10 mm).
• Easy-to-use NUview
Windows
provided with each instrument.
• Easy access to reagent and waste bags and internal battery pack for field replacement.
• Good battery life (approx. 70 days at a 1 hour sample interval at 20 C) with internal D-cell battery
pack. Also can be powered externally by 12 V lead acid battery, AC adapter, or data collection
platform for longer deployments.
TM
software for data analysis and custom presentations. Both software packages are
TM
PC software for simple set-up and deployment. Proven EcoWatch for
• Automatic calibration at user-selected interval. Calibration points do not affect data continuity for
sample intervals of 30 minutes and greater.
• SDI-12 and RS-232 interfaces standard for use with computers and data collection platforms.
• Waste collection system included with instrument to avoid cadmium
• Minimal waste volume because of pump design – approximately 7 liters of waste in 30 days at a 1 hour
sample interval.
• CE and Australian C-Tick Compliance
2+
discharge into the environment
3
-
E.
YSI Environmental 7 Model 9600 Nitrate Monitor
• Large, non-volatile memory for data storage.
• System can be used at depths up to 200 feet.
• All firmware and software is user-upgradeable via the YS I Web page.
SECTION 2 GETTING STARTED
This section is designed to quickly familiarize you with the hardware and s oftware components of the YSI
9600 and its accessories. By the end of Section 2 you will have...
Unpacked the 9600 Nitrate Monitor and confirmed that all components are present
Become familiar with the general features and set-up configurations of your 9600
Prepared the required reagent solutions
Filled and installed the collapsible reagent delivery bags and the waste bags
Activated and installed the cadmium column
Assembled the instrument in preparation for a deployment or laboratory test
Successful completion of the above list is essential for you to continue on to Section 3, which focuses on
setting up the hardware to allow a successful computer interface with the 9600. In subsequent sections, you
will learn about how to set up the 9600 to log data during a deployment , deploy the monit or, and manage the
data files which are generated during field studies.
2.1 UNPACKING
When unpacking your 9600 Nitrate Monitor, be certain not to discard any parts or supplies that were
supplied with the instrument. As you proceed through the unpacking process, use the packing list
(available immediately after opening the box) to ensure that all items are included. Finally, be sure to
inspect all assemblies and components for damage. Proceed by the following steps to unpack the 9600:
• Open the flaps at the top of the box and remove the packing list and sealed information packet which
contains several items, including the manual and the software necessary to interface with the 9600 and
analyze the data, and set the items aside.
• After removing the cardboard tray that contains the information packet, remove the 9600 from the
shipping box by grasping the bail and pulling straight up as shown in the picture below. It may be
prudent to have two people present for this initial unpacking step: one to lift the instrument by the bail
and another to pull the box from the assembly. Note carefully that the 9600 weighs approximately 30
pounds with no chemicals present so make certain that the person removing the instrument from the
box is capable of lifting this amount of weight.
YSI Environmental 8 Model 9600 Nitrate Monitor
• After removal from the box, place the 9600 on a flat surface, usually the floor, and inspect it for any
damage that might have occurred in transport. If any parts are damaged or missing, contact your YSI
representative immediately. If you do not know which YSI dealer you obtained your YSI 9600 from,
refer to Section 13 of this manual for contact information.
• If no damage is present, proceed with the removal of the carousel from the outer housing of the 9600
as defined in the steps below:
• Using a large Phillips head screwdriver, remove the four (4) screws from the sides of the instrument
assembly which hold the inner carousel to the outer assembly as shown in the picture below. Make
certain to retain the screws in a safe place for reassembly prior to use of the 9600.
• After the screws hav e been removed, grasp the bail and lift straight up to remove the carousel as shown
in the picture below. If the carousel binds to the outer shell, have a second person hold the outer shell.
Place the carousel in an upright position on the floor or a bench top after removal.
Lift by Bail
Remove these screws
YSI Environmental 9 Model 9600 Nitrate Monitor
• Locate the package containing the cadmium column and remove the box from the carousel. Also
locate the 9615 Syringe Kit which is near the cadmium column. Set these items aside for later use.
• Locate the seven (7) one liter reagent bags that are present in the individual compartments of the
carousel. Note that one of the bags is loose in the compartment and has a tubing assembly attached to
its middle port. This bag should be removed immediately and set aside for later use. Note that the
other six bags are attached to tubing by multicolored threaded fittings. Note also that each bag has a
colored tie-wrap on the top of the container that is coordinated with the color of the fitting to which the
bag is attached.
• As you inspect the reagent bags, note carefully that there is a small (50 mL) plastic bag that is
permanently attached to the side of the internal cylinder as shown in the picture below. This bag
MUST NOT BE REMOVED as it contains silicon oil. The 50-mL bag is designed to expand and
contract with temperature and depth changes to equalize the pressure between the pump chamber and
the water at the site.
Note Permanent
Attachment
Lift straight up by bail
YSI Environmental 10 Model 9600 Nitrate Monitor
• Using only your fingers (no tools), remove each 1 L reagent bag from its color-coded fitting by
grasping the bag-end of the assembly with one hand and turning the nut counterclockwise with the
other as shown in the picture below. Set the bags aside for later use.
• Inspect the detector cell that protrudes from the underside of the pressure manifold as shown in the
pictures below and confirm that it is correct according to your order – either a 9600-02 with a 2 mm
cell or a 9600-10 with a 10 mm cell. Besides the label that is attached to the cell as an identifier, it is
also possible to differentiate the cells by the fact that the 10 mm cell has two tan colored nuts attaching
the tubing while the 2 mm cell has one tan colored nut and one smaller stainless steel fitting.
2 mm Detector Cell 10 mm Detector Cell
• Remove the battery lid by loosening the four (4) knurled screws on the top of the battery lid as shown
in the picture below. If the screws cannot be turned, use any slotted screwdriver to loosen them
slightly and then screw them out until the battery lid can be removed
YSI Environmental 11 Model 9600 Nitrate Monitor
CAUTION: The battery lid screws are captive. Do NOT continue to turn them after the
battery lid can be removed.
• Confirm that the battery pack is present in the compartment, but do NOT connect its battery lead to the
mating connector at this time.
• Replace the battery lid.
After completing the unpacking and inspection sequence outlined above, proceed to Section 2.2 below to
gain a basic understanding of the Model 9600 mode of operation.
Loosen 4 battery lid screws
and lift battery lid straight up
2.2 UNDERSTANDING THE 9600 MODE OF OPERATION
The Model 9600 is designed to allow users to monitor the nitrate concentration in env ironmental water over
long periods of time with minimal maintenance required. It is NOT designed for spot sampling of nitrate
since the minimal interval between samples is 15 minutes. The system operates by the principle of
injection analysis (FIA) using a series of precise low-volume pumps to mix an aliquot of the water at an
environmental site with user-prepared reagents that produce a colored solution. The intensity of the color
is measured in a detector cell using a green light-emitting-diode (LED) as the light source and a photodiode
as the detector and this intensity is proportional to the concentration of nitrate in the sample. The detector
cell is also equipped with a reference photodiode to automatically compensate for any changes in LED
intensity during a study. Because the pumps are low-volume, minimal waste is generated by the 9600 FIA
system during field studies and this waste can readily be captured in the containers supplied to prevent
environmental contamination.
It is important to understand that, in its normal configuration with the cadmium column in place, the 9600
measures the sum of the nitrate AND nitrite in the water being monitored. Normally, however, because
the biological process of nitrification is active at most sites, there is very little nitrite in most environmental
water and therefore the 9600 reports its readings as nitrate. Users who are concerned that there is indeed a
significant quantity of nitrite in the water at their monitoring sites should either carry out studies with the
9600 with no cadmium column in place or should collect water samples at the site and perform laboratory
analyses for nitrite to determine if its presence is having a significant effect on the overall accuracy of the
measurement.
Users interface to the Model 9600 via a PC that is running the YSI software designated as NUview
which is supplied with each nitrate monitor as part of the information packet. This software provides a
simple interface for set-up and deployment of the nitrate monitor that is ideal for the vast majority of
studies. Under the standard set-up routine, the user inputs specific information about the pending
flow
TM
YSI Environmental 12 Model 9600 Nitrate Monitor
deployment, such as study name, sample interval, and the frequency of auto calibration, into a “Wizard”
based interface, with this portion of the set-up taking a minute or less. The Wizard then guides the user
through a predeployment pumping routine which takes approximately 60 minutes to assure that the
instrument is functioning properly and provides error messages if any problem is apparent. After a
successful predeployment test, the user simply starts the deployment and transports the 9600 to the desired
site for deployment. The study can either be started immediately during laboratory set-up of the nitrate
monitor, or delayed by several hours to allow the study to start after deployment. The key is that the
default FIA sequence for the pumps is ideal for the monitoring of nitrate, and no user “programming” of the
9600 hardware is required.
During deployments, a blank (puri fi ed wat e r ) and a standard (made up to a concentration that matches the
typical nitrate values at a particular site) are automatically run at an interval selected by the user. Use of
these periodic blank and standard reagents allows the instrument software to automatically compensate the
data for any instrument drift during deployment and provides an on-board quality assurance methodolog y.
For most deployments, it will be sufficient to run a blank and standard twice during each 24 hour logging
period. For sample intervals of 30 minutes and greater (typical for most deployments), the calibration
reagents are run through the system
The overall length of a deployment is generally limited by a combination of the quantity of reagents present
in the instrument assembly at the beginning of the study and the sample interval chosen by the user. For
example, the reagents will be expended in approximately 30 days if an interval of 1 hour is chosen between
samples, but approximately 60 days if the interval is set to 2 hours.
While most studies will be carried out using the pumping sequence (number of pumping pulses for each
particular pump at a specified time in the overall routine ) for “typical” nitrate-N concentrations in the 0-5
mg/L range, NUview software also allows users who are monitoring water with very high nitrate
concentrations (5-10 mg/L) to select a special pumping sequence to enhance their accuracy.
You now should have a basic understanding of the capabilities of the YSI 9600 and be ready to proceed to
the next section where you will prepare reagents for use in the deployment of the nitrate monitor.
between samples so that no discontinuity occurs in the logged data.
2.3 PREPARING THE REAGENTS
Four different reagent solutions and distilled or deionized water are required for the use of the Model 9600
nitrate monitor. In this section, you will be given instructions on how to prepare these reagents. In
addition, the 9600 utilizes a column of elemental cadmium which must be “activated” prior to use and
instructions for this procedure are also provided.
CAUTION: All chemicals are potentially harmful and should be handled with care. Be sure to
consult the MSDS for each of the chemicals before preparing any of the reagent solutions. It is very
important to wear appropriate protective equipment including safety glasses with side shields and
use disposable gloves when handling solid chemicals and when preparing and transferring solutions
of these chemicals.
2.3.1 PURCHASING THE REAGENTS
The sources used for the various reagents that have been used in YSI development studies on the 9600 are
provided below. However, users can purchase reagents from any source of their choosing as long as the
chemicals are (a) identical in formulation and (b) equivalent in purity – ACS Reagent Grade or better. An
appropriate quantity of the reagents should be purchased based on the frequency of deployment of your
9600.
YSI Environmental 13 Model 9600 Nitrate Monitor
Ammonium Chloride – Fisher Scientific, Item Number A661-500
Sulfanilamide – Sigma , Item Number S-9251
N-(1-napthyl)ethylenediam i ne dihydrochloride (NED) – Aldrich, Item Number 22,248-8
Potassium Nitrate -- Alfa Aesar Chemical, Item Number 13443
Hydrochloric Acid (10 % v/v; 1.2 M) -- Fisher Scientific, Item Number LC15070-6 (10 liter container)
Copper Sulfate Solution (10 % w/v) -- Spectrum Chemical Mfg. Corp., Item Number C-283
BRIJ 35 Surfactant, Sigma -- 430AG-6
Kodak Photo-Flo 200 Wetting Agent -- Warehouse Photographic, Carrollton, TX, 800-400-8203,
www.warehousephoto.com, 146-4502. Alternatively, this chemical can be purchased at most camera
stores or photo supply houses.
2.3.2 REQUIRED EQUIPMENT AND CONTAINERS
Before proceeding with solution preparation, YSI recommends that you first make sure that you have
available the necessary equipment and storage vessels. First, you should have the proper bottles for storage
of at least 1.5 liters of each reagent. The plastic bottles recommended by YSI for this purpose are 2 liters
in capacity and are graduated so that 1.5 liters of solution can be prepared to the required accuracy for most
of the reagents. These bottles can be purchased from Fisher Scientific in packs of 6 or 12 as Fisher Item
Number 03-311-3g. Second, for preparation of the nitrate standards in various concentrations, you will
need a 1000 mL glass volumetric flask to obtain the desired accuracy. Third, users will require an
adjustable volume pipet for the 0 to 1 mL range such as the Brinkman Eppendorf model which can be
ordered from Fisher Scientific under Item Number 05-402-50. Fourth, if you wish to prepare nitrate
standards above 2 mg/L, you will need glass pipets with the appropriate volumes. For example, to prepare
a 5 mg/L nitrate standard, you will need a 5 mL glass pipet. Finally, users will require a balance that will
weigh accurately to at least 0.01 gram and preferably one which has an accuracy of 0.001 gram. In
summary, the following parts are required prior to reagent preparation:
• Graduated Plastic Bottles with 2 L capacity (at least 6)
• Volumetric Flask for 1000 mL (at least 1)
• Adjustable pipetter for 0-1 mL volumes (at least 1)
• Glass pipet s of va ri o us deno minations (depending on standard concentrat i ons req ui re d)
• Laborato ry bal a nce wit h wei ghi n g accuracy of 0.0 1 g
2.3.3 SOLUTION PREPARATION
The following instructions should be followed to prepare the aqueou s solutions that will be required for use
with the 9600 Nitrate Monitor. At least 1.5 L of the reagents and 3 L of the final standard should be
prepared in order to have enough solution volume to set up the nitrate monitor properly for a deployment.
CAUTION: Be sure to consult the MSDS for each chemical before proceeding and use safe
laboratory practices as outlined above during their handling.
BUFFER – Weigh 128 g of ammonium chloride to the nearest gram and place the solid in a 2 L graduated
plastic bottle. Add 1.5 L of purified water (distilled or deionized) and stir or shake until the solid is
completely dissolved. Using the adjustable pipetter, add 75 uL of 10 % copper sulfate solution and shake
well to mix.
YSI Environmental 14 Model 9600 Nitrate Monitor
SULFANILAMIDE – Weigh 15 g of sulfanilamide to the nearest gram and place the solid in a 2 L
graduated plastic bottle. Add 1.5 L of 10 % hydrochloric acid and shake or stir until all of the solid is
dissolved. Using the adjustable pipetter, add 1.5 mL of BRIJ 35 surfactant.
NED – Weigh 1.50 g of N-(1-napthyl)-ethylenediamine dihydrochloride (NED) to the nearest 0.01 g and
place the solid in a 2 L graduated plastic bottle. Add approximately 1.5 L of purified water and shake or
stir until the solid is completely dissolved. Then add 3.75 mL of Photo-Flo 200 and sh ake well to mix.
NOTE CAREFULLY: The NED reagent is light sensitive and the storage bottle should be either
kept in the dark or covered with some material, e.g., aluminum foil, to prevent premature
degradation.
WASH – Place 1.5 L of water in a 2 L graduated plastic bottle. Add 3.75 mL of Photo-Flo 200 and shake
well to mix.
CAUTION: It is CRITICAL for proper operation of the 9600 for the Photo-Flo 200 to be included in
both the NED and Wash reagents. Do not attempt to deploy without adding the Photo-Flo to these
solutions.
1000 MG/L NITRATE-NITROGEN STANDARD – Weigh 7.22 g of potassium nitrate to the nearest
0.01 g and quantitatively transfer the solid to a 1000 mL volumetric flask. Add approximately 500 mL of
purified water and swirl the mixture until the solid is completely dissolved. Then add additional water to
fill the flask to the top graduation mark. Mix well by repeated inversion of the flask. Transfer the
standard to a dry 2-L plastic bottle for storage. YSI recommends that this standard be stored in a
refrigerator and then warmed to ambient temperature prior to use. If refrigerated, the standard will be
stable for approximately 3 months.
VARIABLE NITRATE-NITROGEN STANDARDS – Nitrate-nitrogen standards in various
concentrations can be prepared by diluting the 1000 mg/L standard prepared in the section above. Using
this method, accurate aliquots of the concentrated standard are transferred to the 1000 mL volumetric flask
and then the flask is filled to the top graduation mark and inverted repeatedly to assure good solution
homogeneity. Examples of the amount of 1000 mg/L standard to dilute to 1000 mL are shown in the Table
below:
Final Nitrate-N Standard Conc Amount of 1000 mg/L Standard Final Volume
6 mg/L 6.00 mL 1000 mL
4 mg/L 4.00 mL 1000 mL
2 mg/L 2.00 mL 1000 mL
1 mg/L 1.00 mL 1000 mL
0.50 mg/L 0.50 mL 1000 mL
0.20 mg/L 0.20 mL 1000 mL
0.10 mg/L 0.10 mL 1000 mL
NOTE: Users can also purchase 1.0 mg/L nitrate-N standard from YSI in 500 mL quantities as Model
Number 3885.
SELECTING A NITRATE STANDARD CONCENTRATION – For your initial deployment using
either a 9600-02 (2 mm detector cell) or a 9600-10 (10 mm detector cell), YSI recommends using a
standard that is 1mg/L in nitrate-nitrogen (nitrate-N). This standard will assure readings that are accurate
to 5 % of the reading or 0.2 mg/L whichever is greater, with the 9600-02 and 5 % of the reading or 0.02
mg/L, whichever is greater, with the 9600-10. Once you obtain more information about the typical high
and low limits of nitrate concentration at a particular site, you might want to use standards of different
values to maximize accuracy. For example, with a 9600-02, if the water at your site is known to
YSI Environmental 15 Model 9600 Nitrate Monitor
consistently contain concentrations of nitrate higher than 6 mg/L, then users might consider employing a
higher standard concentration such as 6 mg/L nitrate-N. For many estuarine and near coastal studies,
where nitrate concentrations may always be below 0.5 mg/L nitrate-N, the use of a 0.2 mg/L standard will
probably improve the data accuracy. Note, however, that YSI does not recommend using standards less
than 0.1 mg/L of nitrate-N.
CAUTION: Use of standards with concentrations higher than 8 mg/L of nitrate-N may compromise the
accuracy of the 9600.
NOTE: The procedure noted above for the variable nitrate-nitrogen standards will have to be carried out
multiple times in order to generate enough standard to fill the two reagent bags as described below.
Alternatively, a larger volumetric flask can be used in the preparation as long as the amount of 1000 mg/L
standard shown in the table is increased appropriately.
After preparing the reagent solutions, be sure to label each storage bottle appropriately.
BLANK– The blank is typically purified water which has a salinity which is roughly that of the water at
the site for brackish and oceanographic applications and simply deionized or distilled water for freshwater
applications.
2.4 FILLING THE REAGENT BAGS
Under deployment conditions, chemical reagents are fed into the FIA system of the Model 9600 from
collapsible reagent bags that you unpacked and set aside in Section 2.1 above. Thus, to operate the nitrate
monitor, you must transfer solutions from the storage bottles to the bags prior to each deployment. The
bags are nominally 1 L, but will in fact require approximately 1.2 L of solution for complete filling and
maximum deployment times.
CAUTION: Be sure to wear appropriate protective equipment including safety glasses and gloves
while filling the reagent bags. In addition, be certain to point the reagent bag fittings away from
your face when you are expelling air from the bag through the partially-open fittings as described
below.
NOTE: The reagent bags are color-coded to match the threaded fittings on the nitrate monitor tubing to
facilitate installation. Be sure to put each reagent in the proper color-coded bag according to the
information in the table below:
REAGENT BAG COLOR
Standard – 2 bags White
Blank – Purified Water Black
Buffer Blue
NED + Photo-Flo 200 Red
Sulfanilimide + BRIJ Yellow
Wash – Purified Water + Photo-Flo 200 Green
It may also be prudent for the user to label each bag with a “magic marker” prior to filling.
NOTE: For the Predeployment Check of the analyzer as described in Section 4.2 below, you will need
TWO 1 liter bags filled with your standard solution. Note that you have been furnished with two
bags labeled with a white tie-wrap, one of which has a tubing assembly attached.
YSI recommends that the method outlined below be used to fill each of the reagent bags:
YSI Environmental 16 Model 9600 Nitrate Monitor
• Locate the 60 mL plastic syringe that was supplied with your Model 9600 in the 9615 Syringe Kit and
remove the plunger. Clamp the syringe to a ringstand with the Luer Lock bottom fitting “down” and
the syringe opening “up”.
• Remove the Luer Lock plug from the side port on the reagent bag and make sure that the pinch clamp
on the middle port of the bag is open. CAUTION: Make sure that you save the side port plug for
resealing the bag after filling.
• Attach the reagent bag via the side port to the Luer Lock fitting at the bottom of the syringe and tighten
securely. See the pictures below for the proper set-up to begin filling reagent bags.
• Make sure that the pinch clamp on the middle port of the bag is in the “open” position.
• Pour the appropriate reagent into the 60-mL syringe and allow it to drain into the reagent bag. Repeat
the addition of reagent until the bag is full and reagent overflows slightly out of the middle port of the
bag.
• Close the pinch clamp on the middle port of the reagent bag so that no solution can escape via this
route.
• Carefully remove the syringe from the side port of the reagent bag and seal the side port with the plug
which was set aside above. With the bag completely sealed, invert the bag back and forth several
times to loosen any bubbles that might be present.
• Now hold the bag in your hand with the connectors “up” and move it slightly back and forth until any
bubbles present are near the side Luer Lock fitting. Loosen the Luer Lock plug slightly (Do no t
remove it) and squeeze the bag gently to expel any bubbles out through the port as shown in the picture
below. Then quickly retighten the Luer Lock plug. See the picture below.
YSI Environmental 17 Model 9600 Nitrate Monitor
The reagent bag is now ready for installation in the nitrate analyzer.
Repeat the filling process for all six reagent bags. Note that the wash (green fitting) and blank (black
fitting) bags should be filled with purified water only.
NOTE: Be sure to rinse the 60 mL syringe with purified water before switching reagents to avoid cross
contamination in the reagent bags.
2.5 PREPARING THE CADMIUM COLUMN
The cadmium column that was set aside during the unpacking process in Section 2.1 is a key ingredient in
the Model 9600 function and MUST be activated prior to use in the nitrate monitor. Activation should be
carried out according to the instructions below.
CAUTION: Cadmium, according to the USEPA, is toxic and a probable human carcinogen. Be sure
to handle the column and perform the activation process in an approved fume hood. Be sure to
wear appropriate personal protective equipment including eye protection and wear gloves while
handling the column and performing the activati on process. Failure to observe these precautions
may lead to potentially serious health problems.
To activate the cadmium column, you must locate the 10-mL plastic syringe assembly that is part of the
9615 Syringe Kit that was supplied with the nitrate monitor. Note that the syringe is fitted with tubing and
a threaded fitting. This syringe is used to push reagents through the cadmium column during the activation
process. You will also require the following reagents that were prepared or purchased above:
• Hydr ochloric Acid (10 % v/v)
• Ammonium Chloride Buffer Solution
• Copper Sulfate Solution (10 % w/v)
Once the reagents and the 10 mL syringe assembly have been located, you are ready to proceed to the next
step.
2.5.1 PREPARING THE COPPER SULFATE REAGENT
The copper sulfate solution (10 % w/v), purchased as described in 2.3.1 above, MUST be diluted prior to
its use in cadmium column activation. Perform the dilution by placing 2 mL of the purchased 10 % (w/v)
YSI Environmental 18 Model 9600 Nitrate Monitor
reagent in a beaker or other suitable container and adding 8 mL of purified water. The resulting 2 % (w/v)
solution will be used as part of the cadmium column activation process.
2.5.2 CADMIUM COLUMN ACTIVATION
CAUTION: In case an inadvertent leak occurs during the following procedure, it is prudent to
perform all operations with the apparatus placed in a flat glass baking dish with dimensions of about
9” x 18 “ x 1”. This procedure allows all leaks to be contained and allows for proper cleanup and
disposal of any spilled reagents.
Working in an approved fume hood, open the box and remove the cadmium column assembly – shown
•
in the picture below.
• Wearing appropriate protective equipment, remove the threaded plugs at the ends of the column
assembly and set aside for later reassembly.
• Place about 15 mL of 10 % (v/v) hydrochloric acid in a beaker or other suitable vessel. With the
syringe plunger fully depressed, place the threaded fitting at the bottom of the syringe assembly in the
reagent and withdraw 10 mL into the syringe by pulling back on the plunger.
• Connect the threaded connector on the syringe assembly to one end of the cadmium column and
tighten by hand – no tools should be used. Mark the direction of flow that you have chosen through
the column by using a marker to place an arrow on the cadmium column assembly.
• Hold the open end of the cadmium column over a beaker or other suitable vessel and then depress the
plunger on the syringe assembly to push the acid steadily through the column as shown in the picture
below. The entire injection process should take approximately 15 seconds.
YSI Environmental 19 Model 9600 Nitrate Monitor
• Rinse and fill the syringe assembly with 10 mL of purified water and repeat the injection procedure
through the column to rinse out the acid. Again, the injection process should take approximately 15
seconds.
• Rinse the syringe assembly with purified water and fill it with the 2 % (w/v) copper sulfate solution
prepared in 2.5.1 above. Connect the syringe assembly to the cadmium column and slowly inject 5 mL
of the reagent into the column while holding the open end of the column over a beaker. The injection
of the 5 mL aliquot of reagent should take approximately 30 seconds. Do not remove the syringe
assembly (which still should contain 5 mL of solution) from the cadmium column .
• Reinsert the threaded plug in the open end of the column assembly.
• Set the entire assembly aside for at least 15 minutes to allow proper activation. No damage to the
column will occur if the assembly is left for several hours in this state.
• After the initial activation, remove the threaded plug from the open end of the column, slowly inject
the remaining 5 mL of the copper sulfate solution into the cadmium column and again set the assembly
aside for 15 minutes or longer after reinserting the plug in the open end of the column.
• Remove the syringe from the assembly and rinse it with purified water. Remove the threaded plug
from the other end of the column assembly .
• Fill the syringe with 10 mL of the ammonium chloride buffer solution which was prepared as
described in 2.3.3 above. Inject all of the buffer reagent through the cadmium column at a rate so that
the entire injection takes about 15 seconds.
• Remove the syringe from the column assembly and reinsert the threaded plugs in each end of the
assembly.
The cadmium column is now activated and ready for installation.
2.6 INSTALLING THE CADMIUM COLUMN
The next step in setting up for deployment is to install the cadmium column in the carousel of the nitrate
analyzer. Remove the carousel from the outer shell of the analyzer and place it on the floor or a bench top
in the vertical position supported by its three legs.
YSI Environmental 20 Model 9600 Nitrate Monitor
g
Locate the two black straps in one of the carousel segments that will be used to retain the cadmium column
assembly. Note that there is a section of tubing within the straps with two ends with brown threaded
fittings that are connected by a translucent nut.
NOTE: For optimum performance, the cadmium column should be installed in the flow system of the
carousel so that the direction of flow through the column is the SAME as that used in its activation.
To make sure that the direction of flow through the cadmium column is correct, note that one of the
sections of tubing within the carousel straps tubing goes to a circular tan fitting with two other pieces of
tubing connected to it. This fitting on this section should be attached to the OUTLET of the cadmium
column as marked during the activation process. See the picture below left to identify the tubing nut that
goes to the cadmium tube outlet.
Loosen the straps by pushing up on the tab and pulling on the strap as shown in the picture ab ove right.
Remove the translucent coupling nut so that the two threaded brown fittings are free. As noted above,
identify the threaded nut that goes to the circular tan adapter. Remember that this nut should be attached to
the OUTLET of the cadmium column assembly.
Screw the threaded fittings on the tubing ends into the ends of the cadmium column assembly after
removing the plugs from the column. Use only finger tightening to make the connection – no tools should
be used.
Align the connected cadmium column assembly vertically within the strap clamps and pull the end of the
straps so that the column assembly is held in place firmly as shown in the picture below. Note that the
straps should fit within the indentations on the cadmium column assembly.
Outlet
CAUTION: Be certain to retain the two black plugs that were removed from the cadmium
column prior to its installation. These plugs will be required to seal the column assembly
for proper disposal after its reducing capacity has been expended.
Circular Fittin
YSI Environmental 21 Model 9600 Nitrate Monitor
Now proceed to the installation of the reagent bags as described in Section 2.7 below.
2.7 INSTALLING THE REAGENT BAGS
The next step in setting up the Model 9600 for field use is to install the six 1-liter reagent bags containing
solutions prepared in Section 2.4 above into the nitrate monitor carousel. Remove the carousel from the
outer shell of the analyzer and place it on the floor or a bench top in the vertical position supported by the
three legs. Then follow the step-by-step instructions below to install the reagent bags.
• Locate the six lines of reagent tubing which have color-coded threaded fitting on the ends. The ends
of the tubing with threaded fittings will be found hanging below the middle plate of the carousel and
should be retrieved manually. Note that these are the only loose fittings within the carousel, so their
identification should not be a problem.
• Note that there are three compartments in the top section of the carousel and that each compartment
must hold two reagent bags. The bags will be placed on the hooks that are hanging from the top plate
of the reagent compartments.
• Retrieve one of the fittings which is hanging in the carousel, note its color and then locate the filled
reagent bag with the same colored tie-wrap on its middle port. Make sure that each bag has the proper
reagent as shown in the table below. As long as the color code was followed in the filling of the
reagent bags, no possibility of error exists at this step. Make sure that the recovered tubing is routed
through the hole in the middle of the bottom plate rather than around the edges.
REAGENT BAG COLOR
Standard – 2 bags White
Blank – Purified Water Black
Buffer Blue
NED Red
Sulfanilimide Yellow
Wash – Purified Water Green
• Make the tubing to reagent bag connection by screwing the threaded fitting on the tubing into the
middle port of the reagent bag. Tighten securely with fingers only – no tools should be used.
• Hang the connected bag from one of the six hooks in the upper plate of the carousel.
• Repeat the sequence for the remaining five reagent bags making sure that t here are tw o b ags hanging
in each compartment, one on each hook.
• Open the pinch clamps on all bags and flick the middle port of each of the bags with your finger to
cause any remaining air bubbles to move to the top (closed end) of the reagent bags as shown in the
picture below.
YSI Environmental 22 Model 9600 Nitrate Monitor
Flick fitting to force
bubbles to top of bag
2.8 USING THE WASTE BAGS
As received by the user, the two 5 liter waste bags will already be connected and reside in the bottom
compartment of the carousel. However, YSI sugg est s that u sers become acquainted with the routing of the
tubing to the bags from the pump manifold so that reattachment after each deployment will be easier.
Note that each waste bag is enclosed in a plastic net that will support the weight of the liquid waste during a
deployment. The net material is an integral part of the waste bag assembly and should NOT be removed.
The waste bag assembly is attached via the metal clips to two hooks on the bottom of the carousel’s middle
plate. Each of the two waste bag assemblies should be attached to two different hooks for proper function.
Note also that a single waste line from the manifold connects to a “T” fitting which distributes the effluent
from the system between the bags as shown in the picture below. Because the flow path to one bag may be
slightly more facile than the other, it is not uncommon for effectively all of the waste to accumulate in a
single bag until it is full and then the waste stream shift to the other bag. Users should not be concerned by
this phenomenon.
2.8.1 REMOVING THE WASTE BAGS
To remove the waste bags, place the carousel on the floor or on a bench in a vertical orientation, supported
by its three legs. Then follow the step-by-step instructions below:
From Pump Manifold
T-Fitting
To Waste Bags
YSI Environmental 23 Model 9600 Nitrate Monitor
• Make certain that the pinch clamps on the two waste bags are CLOSED.
• Remove the threaded fittings from the middle ports of the waste bags.
• Remove the waste bag assemblies (waste bag enclosed in netting) from the hooks on the underside of
the center plate of the carousel.
• Set the waste bag assemblies aside for proper disposal of the waste.
CAUTION: The waste solution will contain potentially hazardous organic compounds, hydrochloric
acid, and, most importantly, cadmium ions (Cd
carcinogen. Users should be aware of two important warnings when dealing with this waste
solution:
• Be certain to wear appropriate personal protective equipment including disposable
gloves and proper protective eyewear when handling the waste bags and the waste
solution.
• Be certain to dispose of the waste properly. Unless specifically allowed by your local
wastewater authority, the waste should NOT be emptied into a sanitary sewer, i.e. a sink
drain. Instead, the waste should be collected in a suitable container and disposed of
with an approved waste handler. This waste handler should be contacted BEFORE you
accumulate waste in laboratory or field studies so that the proper storage vessels are
available and a schedule for waste pick-up has been arranged.
• To empty each bag into the appropriate container, invert the bag so that the port is in the “down”
position and is over the waste container. Then carefully open the pinch clamp and allow the waste
solution to flow out of the bag. Use gentle pressure on the bag to facilitate the process and make sure
that all of the solution has been removed. Note, however, that it is not important to rinse the waste
bag before reinstallation.
2.8.2 ATTACHING THE WASTE BAGS
To install the waste bags prior to deployments, place the carousel on the floor or bench in a vertical
position supported by its three legs, as shown in the pictures above, and follow the step-by-step instructions
below.
• Open the pinch clamp on the port of the waste bag and place the bag assembly on a flat surface.
• Place your hand on the closed end of the bag and move it across the bag toward the end with the
fitting, squeezing out any air through the pinch clamp fitting.
• Close the pinch clamp.
• Locate one of the tan-colored threaded fittings on the waste lines and attach it to the pinch clamp port
of the waste bag. Tighten securely with fingers only – no tools sho ul d be use d.
• Hang the empty waste bag assembly (waste bag in netting) on two of the hooks on the underside of the
center plate of the carousel.
• Install the second waste bag repeating the steps above.
2+
) which are toxic and a potential human
YSI Environmental 24 Model 9600 Nitrate Monitor
• Open the pinch clamps on the ports of the waste bags.
2.9 ASSEMBLING THE NITRATE MONITOR
Before assembling the Model 9600 nitrate monitor for deployment, make sure the following has been done:
• The cadmium column has been activated properly and installed in the carousel.
• The six reagent bags have been filled, attached to the proper connectors, are properly installed, i.e.,
hanging from individual hooks in the carousel, and all of their pinch clamps have been OPENED.
• The two waste bag assemblies are properly attached to the effluent lines and the metal rings at the tip
of each assembly are attached to the hooks on the bottom of the carousel center plate. OPEN the
pinch clamps on the waste bag assemblies.
CAUTION: Make absolutely certain that the pinch clamps on all of the bags (reagent and waste) are
OPEN so that fluid can be pumped within the system. Failure to open ALL pinch clamps will result
in total failure and may damage the pumps.
After this check list has been completed, assemble the 9600 by following the instructions below:
• Place the outer shell of the monitor on the floor near the carousel.
• Lift the carousel by the bail, raising it above the top of the outer shell as shown in the picture below:
• Slide the carousel into the outer shell making sure that the two rectangular indentations on the top plate
of the carousel are aligned with the black strips on the outside of the outer shell as shown in the
pictures below. Note carefully that the inlet filter should be on the side of the strip that has two
YSI Environmental 25 Model 9600 Nitrate Monitor
eyebolts attached to it. The picture on the left below shows th e correct orientation; the one on the
right shows the incorrect placement of the carousel within the outer shell.
CAUTION: Make certain that no sections of tubing are pinched between the outer shell and the
carousel during the assembly process.
• With the carousel supported by the outer shell and the top plate slots aligned with the outer shell strips,
press down on the top plate until the outer shell is seated in the circular slot on the carousel top plate
and the four (4) side screw holes in the strips on the outer shell are aligned properly with the holes in
the top plate.
• Install the four screws that were set aside in the disassembly process described in Section 2.1 above.
Use either a standard Phillips head screwdriver for this process or a battery powered driver with a
Phillips bit.
CAUTION: If a power driver is used, make sure that it is on a minimal torque setting to avoid
overtightening the screws.
CAUTION: The eyebolts which are used to attach the bail to the top of the carousel are DESIGNED
TO BE AT AN ANGLE as shown in the picture below. Do NOT attempt to straighten the alignment
of the eyebolts.
Correct
Incorrect
DO NOT CHANGE ALIGNMENT
OF EYEBOLTS
YSI Environmental 26 Model 9600 Nitrate Monitor
SECTION 3 PREPARING FOR A 9600 DEPLOYMENT
In Section 2, you set up the physical components of your Model 9600 – cadmium column, reagent bags,
and waste bags. In this section, you will learn how to install the PC software which allows interface to
your 9600, power your instrument with either a battery pack or a power supply, attach a cable to the 9600,
and establish an interface between the nitrate monitor and your PC.
3.1 INSTALLING NUVIEW PC SOFTWARE
Locate the NUview CD-ROM which was shipped as part of your 9600 package and which you unpacked in
Section 2.1 above. The CD contains a file that will install software to your PC that will allow you to interface
to the 9600 in order to:
• Configure y our inst rument for a depl oyment
• Start a depl oyment
• Interact wit h the 9600 during depl oyment s to upload data
• Stop a deployment
This software is designated NUview and the Model 9600 can ONLY be operated from a computer which is
running this software.
3.1.1 SYSTEM REQUIREMENTS
NUview software will run properly on computers with the following characteristics:
• IBM Compatible PC
• Windows
• 32 MB memory
• 20 MB available disk space
• CD-ROM Drive
• RS-232 Serial Port
• Monit o r ca pable of 800 x 600 resolution or better
3.1.2 SOFTWARE INSTALLATION
To install NUview software to your computer, perform the following steps:
• Place the NUview CD-ROM into the CD drive of your computer.
• Use Windows Explorer or My Computer to view files on the CD-ROM. Double-click on the
The display will indicate that the set-up routine is proceeding. Follow the instructions on the screen as the
installation proceeds. For most applications, the default settings should work without problems. After
installation, there is no need to restart the computer.
®
2000, or XP
Setup.exe file.
YSI Environmental 27 Model 9600 Nitrate Monitor
3.2 CHOOSING A CABLE FOR THE 9600
Depending on your applications, several YSI cables can be used to interface your 9600 to a computer
running NUview software. The use of each of the cable types is explained below.
3.2.1 MODEL 6067B LABORATORY CABLE
The 6067B is a short cable with a slide-in connector for the 9600 communication port on one end and a
DB-9 (for serial computer connection) on the other. The cable also has an MS-4 connector which can be
used to input power to the 9600 from the optional 9689 power supply – see section 3.3.2 below for more
information on the 9689. If your applications involve the remote deployment of the 9600 where the
instrument is powered on internal batteries and then recovered for data upload, then the relativelyinexpensive 6067B is probably the ideal cable interface to your 9600. The cable can be used in the
laboratory to set up the instrument for deployments as described in Section 4 below and to upload data in
the laboratory or in the field when no provision for a water tight connection to the 9600 is required.
However, users should note the following limitation of the 6067B before choosing to purchase this cable
for use with the 9600:
• The connection between the cable and the 9600 is NOT watertight and so the cable cannot remain in
place for field deployments
To use the 6067B with your 9600, simply align the keys on the “sonde” end of the cable and the
communication port on top of the 9600 and press the connectors together. Then plug the DB-9 end of the
connector into a serial port on a computer running NUview software. To power the instrument using the
9689 power supply, connect the MS-4 connector on the 9689 to the mating connector on the cable.
3.2.2 STANDARD YSI 6-SERIES CABLES
Users of the 9600 who also have YSI 6-series field cables, su ch as the Models 6090, 6091, 6092, or 6093,
can utilize these cables for interface to the 9600 under limited conditions. These cables have a silver,
water-tight “sonde” connector with a knurled nut on one end and a smaller military-style (MS) connector
on the other end. When using these cables to interface to the 9600, the optional YSI 6095B MS-8 to DB-9
adapter must be connected to the MS-8 end of the cable. Place the MS-8 ends of the cable and adapter
together, and rotate until the alignment pins engage and the male and female portions of the connector slide
together. Once the bodies of the connectors are fully engaged, twist the knurled ring on the cable until the
two pieces lock together.
Once the cable is assembled, attach the knurled silver connector to the communication port on the top of
your 9600. Rotate the knurled end until the metal “key” engages and then tighten the connector by turning
the knurled nut clockwise using fingers only – no tools should be used.
Connect the DB-9 end of the cable assembly to a serial port of the PC on which you have installed NUview
software in Section 3.1 above.
CAUTION: Users with existing 6-series field cables should be aware of the following limitation for use
with the 9600.
• The 9600 CANNOT be powered by auxiliary devices such as the 9689 power supply, 12 volt lead acid
batteries, or data collection platforms during deployments using these cables. The current draw when
the 9600 is actually making measurements is too great for the wires in the cable.
YSI Environmental 28 Model 9600 Nitrate Monitor
3.2.3 MODEL 9600 FIELD CABLES
As noted in Sections 3.2.1 and 3.2.2 above, the major limitation of the use of existing YSI cables with the
9600 is that the nitrate monitor CANNOT be powered externally through these cables during deployments
using the 9689 power supply, auxiliary batteries, or data collection platforms. However, special field
cables such as the 9691, 9692, and 9693 are available for use with the 9600 under these conditions. These
cables utilize heavier gauge wires than the standard YSI cables such that the 9600 can be remotely powered
for distances of up to 100 feet between the power supply and the nitrate monitor.
NOTE CAREFULLY: The 96XX series field cables can be identified by the YELLOW plastic
overmold on each of the connectors. The standard YSI 6-series cables have BLACK plastic
overmolds on the connectors.
As described above for the standard 6-series cables in Section 3.2.2, users must utilize a 6095B adapter
with the 96XX cables in order to interface to a computer running NUview software
Remember that if you will be powering your 9600 IN THE FIELD USING AUXILIARY POWER OF
ANY TYPE you will need to use a 96XX field cable – a standard 6-series cable will NOT function in this
mode.
Once you have selected the cable that is suitable for your application, proceed to the next section on
applying power to the 9600.
3.3 POWERING THE 9600
3.3.1 INSTALLING AND USING THE BATTERY PACK
For remote deployments, the Model 9600 operates off a factory sealed battery pack containing 14 alkaline
D batteries operating at an initial voltage of approximately 10.5 volts. A battery pack has been shipped
with your 9600 and will be found in the battery compartment as described above in the unpacking
instructions.
NOTE: For initial set-up of the 9600, YSI recommends using the battery pack as described below.
However, if you have purchased the Model 9689 power supply, you may also use this item to power the
instrument during the set-up process. If you wish to use the 9689 in the absence of batteries, proceed to
Section 3.3.2 below.
CAUTION: Users should use ONLY battery packs which have been purchased from YSI for use with
the 9600. Use of alternative battery packs could damage the instrument.
To properly install the Model 9600 battery pack, follow the step-by-step instructions belo w:
• With the assembled Model 9600 in a vertical position on the floor or a benchtop, locate the battery lid
on top of the instrument as shown in the picture below left:
YSI Environmental 29 Model 9600 Nitrate Monitor
• Using your fingers only, loosen the four captive screws on the battery lid until they turn freely.
NOTE: If the screws are too tight to remove by hand, it is permissible to use a slotted screwdriver to
loosen them.
• Grasp the handle on the battery lid and pull straight up to remove it, exposing the battery compartment
as shown in the picture above right. Set the lid aside for later reassembly.
• Locate the hexagonal battery pack that is stored in the battery compartment and is shown in the picture
above right. Note that there is a cable attached to the battery pack with a 2-wire connector.
• Note that there is also a cable coming out of the side of the battery compartment that also has a mating
2-wire connector.
• Connect the two 2-wire connectors together to power the instrument from the battery pack.
• Replace the battery lid and tighten the four screws using your fingers only.
IMPORTANT: If the two battery cables are connected, then the batteries are slowly being
discharged even if the instrument is not being used actively. Therefore, for periods of inactivity
lasting more than a few hours, YSI recommends that you remove the battery lid and disconnect the
two connectors in order to maximize the lifetime of your battery pack.
Battery Lid
CAUTION: The battery lid screws are captive. Do NOT continue to turn them after the
battery lid can be removed.
Battery Pack
3.3.2 USING THE MODEL 9689 POWER SUPPLY
Although the Model 9600 has internal batteries, using the optional Model 9689 power supply for laboratory
studies and for instrument calibration and set-up is often convenient and extends battery life. The 9689 will
automatically convert line voltages of 90 to 264 VAC to 12 VDC and allows for the use of line input cords
from most countries world-wide. The Model 9689 is supplied with an American/Canadian cord.
To use the 9689, attach the four-pin connector from the power supply to the mating connector on the 6067B
cable or the 6095B adapter when using a field cable. Twist the connectors together and then simply plug
the power cord into the appropriate AC outlet. The 9600 is equipped with reverse voltage protection so
Lift
YSI Environmental 30 Model 9600 Nitrate Monitor
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