The YSI 9300 direct-reading photometer is designed to give long and troublefree operation. To ensure the best results, please read these instructions
carefully and follow the procedures recommended.
The photometer is suitable for use in both the laboratory and the field. It is
rugged and durable but should be regarded as a scientific instrument. It is
designed to resist moisture and spills but care must be taken to maintain the
life of the instrument.
Keeping the photometer clean, free from contamination and in good working
order :-
1 Do not pour out samples or prepare the tests directly over the instrument.
2 Always cap the test tubes after preparing the blank and test sample.
3 Wipe test tubes with a clean tissue to remove drips or condensation
before placing in the photometer.
4 Do not leave tubes standing in the photometer test chamber. Remove
the tubes immediately after each test.
5 Immediately wipe up any drips or spills on the instrument or in the test
chamber with a clean tissue.
6 Keep the instrument clean. Clean the test chamber regularly using a
moistened tissue or cotton ball.
7 Keep the instrument away from all chemicals and cleaning materials.
8 Keep the instrument in a clean, dry place when it is not in use. Keep it on
a clean, dry bench away from chemicals, place it in a storage cupboard
or keep it in a carrying case.
9 Keep the carrying case in a clean, dry condition. Make sure that the
carrying case is dry before the case is closed up and the instrument is put
The YSI 9300 direct-reading photometer is an instrument that measures color
intensity. Light is passed through a test tube containing the sample solution, and
then through a colored filter onto a photodetector. Filters have been chosen so that
light of a specific wavelength is selected. When the solution is completely colorless,
all of the light passes through the sample. With colored samples, light is absorbed
and that which passes through the sample is proportionately reduced.
In 9300 test procedures, the direct-reading photometer is used to m eas ure the colo r
which is produced when chemical reagents are reacted with the sample. In these
tests, the color intensity produced is proportional to the concentration of the
parameter under test.
The photometer is pre-programmed with calibrations for each test parameter.
Different test procedures are carried out at different wavelengths to optimize the
sensitivity of each test. The required wavelength is selected automatically by
the instrument.
The calibrations are accessed by entering a unique program number at the
start of each test procedure. This enables the instrument to select the
appropriate wavelength filter automatically and allows the photodiode
response to be converted to a concentration reading. The instrument thus
displays a direct-reading
The photometer is ideally suited for general analytical applications. The
instrument can be used as a laboratory or field photometer for standard
analytical methods or for comparison of colored solutions.
of the test result.
For general analytical applications, Transmittance (test program 0), or
Absorbance (test program 1) can be chosen.
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Power Supply
The photometer is designed to operate on alkaline batteries.
The photometer features a battery indicator – see ‘System Mode’ functions and scroll
down to Battery Level. A minimum voltage of 3.0V is needed to operate the
photometer.
In addition to the above feature, a battery-warning message will appear automatically
on the display when the battery voltage becomes low. The batteries should be
replaced as soon as possible after the warning message appears.
Replacing the Batteries
The battery compartment in the base of the instrument is secured by four screws.
To replace the batteries, remove the screws (set aside), remove the cover and
install the batteries, observing the correct polarity as indicated. Use 3 x 1.5V 'AA’
alkaline batteries.
To avoid potential corrosion damage, remove batteries from the instrument if it is to
be stored or left unused for a long period of time (> 30 days).
GENERAL PHOTOMETER OPERATION
The photometer is controlled by a simple intuitive menu system.
The highlight indicates the active line or section of the screen.
The and keys move the highlight through the menu choices.
The and keys allow selection of options.
The flashing cursor in the ‘options menu’ at the bottom of the screen
indicates the action which will occur if the [OK] button is pressed.
Operating Modes
The photometer has two distinct operating modes - the PHOTOMETER mode
and the SYSTEM mode.
The PHOTOMETER mode is the normal operating mode for taking photometer
readings. This mode is engaged automatically when the instrument is turned on
by pressing the key.
In order to conserve battery life the photometer will switch off automatically
after 5 minutes of non-use.
The SYSTEM mode is used to set the system options. This mode is engaged
when the photometer is turned on using the key and then selecting 'System'
using the
and keys and pressing [OK].
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