OLYMPUS CELL TIRF User Manual [ru]
®
USER INSTRUCTIONS
MITICO(-2L)
LAS-BOB
LAS-MC
MOTORIZED INTEGRATED TIRF
ILLUMINATION COMBINER
LASER SAFETY BREAK-OUT BOX
LASER MANUAL CONTROL
This instruction manual describes the Olympus MITICO illumination combiner with
accessories. To ensure safety, obtain optimum performance and familiarize yourself fully with the
use of this product, we recommend that you study this manual thoroughly before operation.
Together with this manual, please also read the imaging system manuals, the laser manual and
the instruction manual of the microscope in order to understand overall operation methods.
Retain this manual in an easily accessible place near a system for future reference.
OLYMPUS SOFT IMAGING SOLUTIONS GMBH
Johann-Krane-Weg 39
D-48149 Münster
Tel: +49 89 - 89 55 805 660
Fax +49 89 - 89 55 805 6606
Email: info@olympus-sis.com
www.olympus-sis.com
Imaging Excellence
We at Olympus Soft Imaging Solutions GmbH have tried to make the information in this manual as
accurate and reliable as possible. Nevertheless, Olympus Soft Imaging Solutions GmbH disclaims any
warranty of any kind, whether expressed or implied, as to any matter whatsoever relating to this manual, including without limitation the merchantability or fitness for any particular purpose. Olympus Soft
Imaging Solutions GmbH will from time to time revise the software described in this manual and reserves the right to make such changes without obligation to notify the purchaser. In no event shall
Olympus Soft Imaging Solutions GmbH be liable for any indirect, special, incidental, or consequential
damages arising out of purchase or use of this manual or the information contained therein.
No part of this document may be reproduced or transmitted in any form or by any means, electronic or
mechanical, for any purpose, without the prior permission of Olympus Soft Imaging Solutions GmbH.
2010 by Olympus Soft Imaging Solutions GmbH. All rights reserved.
Manual version: September 2010
Illumination Combiner, User Manual Contents
Contents
1
Introduction ................................................................................................. 1
1.1 Abstract ........................................................................................................ 2
1.2 General ......................................................................................................... 2
1.3 Components .................................................................................................3
2
Safety Symbols............................................................................................5
3
Safety Precautions and Handling Instructions .......................................9
3.1 Laser Safety Precautions ............................................................................10
3.2 General Safety Precautions ........................................................................11
4
4.1 Setup Scheme ............................................................................................14
4.2 The Laser Safety Features .......................................................................... 15
4.3 The MITICO Features – an Overview .......................................................... 17
4.4 The LAS-BOB Laser Safety Break-out Box ................................................18
4.5 The Laser Shutter Manual Control..............................................................18
4.6 The AD-USB-ODB Bus Adaptor Box.......................................................... 19
4.7 The Remote Interlock Connector of the Olympus ..........................................
Soft Imaging Solutions ODB Laser Systems ..............................................20
4.8 The Light Path Selector Slider ....................................................................20
4.9 The Iris Diaphragm Field Stops .................................................................. 21
4.10 Laser Clean-up Filter Sliders ...................................................................... 22
4.11 Focusing the Laser Lines............................................................................22
4.12 The FRAP Lens of Laser Line 1 .................................................................. 23
4.13 Laser Adjustment........................................................................................23
4.14 Filters, Mirrors, Objectives .......................................................................... 24
MITICO and Peripherals Overview..........................................13
5
Modes of Operation .................................................................................. 25
5.1 The User Mode ...........................................................................................26
5.2 The Maintenance Mode .............................................................................. 27
5.2.1 Exchanging Microscope or Camera Hardware .........................................28
5.2.2 Adjusting the Laser Beam Position ...........................................................28
5.2.3 Access to the Specimen without Deactivating the TIRFM System .......... 28
5.2.4 Observing the Specimen under TIRF Illumination Through the Ocular ....29
6
Using the MITICO Illumination Combiner ............................... 31
6.1 The cell^TIRF Control Software.................................................................. 32
6.2
6.2.1 System Configuration ............................................................................... 38
6.2.2 Operating MITICO ...................................................................... 39
6.2.3 TIRFM in the Experiment Manager ...........................................................41
7
Specifications and Technical Data.......................................................... 45
MITICO Control via the cell^R Software ..................................... 38
Illumination Combiner, User Manual Chapter 1 –Introduction 1
1 Introduction
Thank you very much for purchasing Olympus Soft Imaging Solutions' state of art motorized
illuminator system, and for your confidence in our products and service. It is Olympus Soft Imaging
Solutions' main objective to provide you with solutions able to meet your experimental demands and
thus pave the way to your scientific success.
The
lasers and a white-light source to an inverted Olympus IX2 microscope and to adjust the position of
each laser beam individually relative to the optical axis to obtain total internal reflection illumination.
illuminator system is designed for general laboratory use only. It allows coupling up to four
1.1 Abstract ........................................................................................................ 2
1.2 General ......................................................................................................... 2
1.3 Components .................................................................................................3
2 Chapter 1 – Introduction
Motorized multi-port
illumination combiner MITICO with fiber port for widefield illumination
1.1 Abstract
In the following sections you will find a detailed description of the components of the
system, handling and alignment instructions and how to maintain the performance of your TIRF microscope. Keep in mind that all components are designed to work together with an Imaging Station like
the Olympus cell^M or cell^R as an integrated system. We strongly discourage taking away or replacing single components without prior consultation of Olympus Soft Imaging Solutions, most probably it
will impair the performance of the system dramatically. The guarantee will not cover any damages to
the system due to such mishandling. All necessary tools for handling and alignment (except the laser
safety goggles) are part of the product.
If you find any information missing in this manual or you need additional support, please contact your
local Olympus representative.
illuminator
1.2 General
The MITICO motorized integrated TIRF illumination combiner is compatible with the Olympus microscopes IX51, IX71 and IX81 as well as a range of laser systems within visible spectral range between
400nm and 700nm provided that they meet the following requirements:
• Every laser unit must have an input remote-control safety interlock that must be connected to the
central laser emergency stop and which interrupts the laser radiation in case of danger (EN 60825-
Illumination Combiner, User Manual Chapter 1 –Introduction 3
1:2003, Sect. 4.4 and 10.2). Furthermore, every laser unit must have a key-activated main power
switch in the sense of EN 60825-1:2003, Sect. 4.5).
• Provided that the laser units can be operated independently they must have their own emission
warning display (audible or visible) (EN 60825-1:2003, Sect. 4.6).
• The combined power of all employed and/or connected laser powers must not exceed 500mW.
This instructions manual describes the hardware components of the
illuminator system, their
installation and alignment. For the use of the connected lasers refer to the respective manuals.
It is important to familiarize yourself with the entire system to ensure safety and optimum performance.
We recommend studying this manual as well as the software and hardware manuals of the imaging
system thoroughly before operating the
illuminator system. Keep this manual close to the imag-
ing station for future reference.
The
illuminator system meets the CE requirements:
• EN61326 Class A
• EN60825
1.3 Components
The TIRF illuminator system consists of the following hardware components:
a MITICO motorized integrated TIRF illumination combiner:
—
Four fiber ports, each with adjustable line focus
—
A port for either a TIR-LHA adaptor to couple a standard fluorescence lamp housing or TIRMTCON to couple a MT10 / MT20 illumination system.
—
A slider to switch between 100% widefield illumination and 50% widefield plus 100% TIRF
—
Motorized drives to move each laser line
—
A field stop for each laser line
—
Removable slider for a clean-up filter for each laser line
—
FRAP focus lens for the first line that can be moved in and out of the optical path
—
Laser safety shutter with key
—
Illumination combiner support
b Laser shutter hand switch LAS-MC with key with emergency interrupter and key-controlled switch
to maintenance mode
c Laser safety break-out box LAS-BOB
d Laser safety stage cover with translucent laser safety glass that allows following the beam position
e Laser safety ocular shutter
f Laser beam alignment tool
g Manuals
The following items are additionally needed to convert a fluorescence microscope into a TIRF microscope:
h Up to four laser systems with a maximum total of 500 mW light output
4 Chapter 1 – Introduction
i – Either a TIR-LHA adaptor to couple a standard fluorescence lamp housing
– or a TIRMT-CON to couple a MT10 / MT20 illumination system
j A widefield illumination system
– Either a standard fluorescence lamp housing, e.g., U-LH100HGAPO
– or a MT10 / MT20 illumination system
Illumination Combiner, User Manual Chapter 2 –
Safety Symbols
5
2 Safety Symbols
The deactivation of the safety systems or the dismantling of different components by the operator is
required in certain operational modes and in case of illumination alignment. As a result the Olympus
motorized
safety class 3B and in accordance to EN 954-1:1997 in hazard category 2. Class 3B lasers are medium power lasers with an output power of up to 500 mW within 400 – 700 nm wavelength. Laser
safety regulations require extensive labeling of all possible apertures where laser radiation might potentially exit including such apertures that are totally covered except upon partially or totally disassembling
the system.
illuminator system is to be classified in accordance with EN 60825-1:2003 in laser
6 Chapter 2 –
Safety Symbols
The following symbols are placed on the Olympus
illuminator system. Study the meaning of the
symbols and always use the equipment in the safest possible manner.
General symbols
Symbol Explanation
Indicates the use of a laser beam. Take special care in handling that part.
(See also following chapter)
Carefully read the instructions on the label and the manual before use. Improper use could
result in personal injury to the user and/or damage the equipment.
This symbol indicates separate collection of waste electrical and electronic equipment in the EU
countries.
Do not throw the equipment into the domestic refuse
Please use the return and collection systems available in your country for the disposal of this
product
Each laser source must be provided with a danger symbol, a sign with the classification text and a sign
on the beam exit opening. Please ensure that your microscope is labeled as shown in the table below.
For all parts labeled with the following labels special laser precautions must be considered (see Chapter 4.1, Laser Safety Precautions)!
Laser safety symbols
Symbol Explanation Location
Indicates the use of a laser beam.
Take special care in handling this part.
Front of the MITICO illumination combiner; see photos 1
Laser radiation can cause irreversible
damage to your eyes. Wear adequate
protection before opening this aperture.
These apertures should be closed
while operating the system. Parts
connected should be dismounted by
trained personal only and when the
laser is turned off.
When system is switched to maintenance mode laser radiation can be
emitted from this aperture. Be sure to
wear adequate eye protection.
Front of the MITICO illumination combiner. May be hidden if the MITICO is
mounted on the microscope; see photo 1.
Microscope frame, right side, left side
near C-mount; see photos 2 and 3.
Laser safety ocular shutter; see photo 4.
Laser safety stage cover; see photo 5.
Illumination Combiner, User Manual Chapter 2 –
Safety Symbols
7
Labels for the microscope components are included in the shipping list of the
illuminator system. Please paste them on the microscope upon system installation. If any label is missing please ask
your Olympus representative for additional labels.
1:
MITICO motorized integrated TIRF illumination combiner
2: microscope frame, right side 3: fluorescence turret and C-mount
4: laser safety ocular shutter 5: laser safety stage cover
8 Chapter 2 –
Safety Symbols
Illumination Combiner, User Manual Chapter 3 – Safety Precautions and Handling Instructions 9
3 Safety Precautions and Handling
Instructions
The Olympus
Class 3B. Class 3B lasers are medium power lasers with an output power of up to 500 mW within 400 –
700 nm wavelength. The total of the output power of the connected lasers may not exceed 500 mW.
Viewing these lasers under direct beam and specular reflection conditions is hazardous. The diffuse
reflection is usually not a hazard except for higher power Class 3B and Class 4 lasers. Normally the
class 3B laser is not a fire hazard. A class 3B laser product may only be used under the control of a
laser safety officer. Before using this product be sure to be familiar with the demanded safety measures
according to EN 60825-1 and the national regulations
illuminator system must be used with laser products belonging to the Laser Safety
3.1 Laser Safety Precautions ............................................................................10
3.2 General Safety Precautions ........................................................................11
10 Chapter 3 – Safety Precautions and Handling Instructions
3.1 Laser Safety Precautions
Caution: Control or adjustments using procedures other than those prescribed in this manual will lead to hazardous laser beam exposure.
To avoid hazardous situations strictly follow the laser safety instructions below.
1. Before operating an Olympus
rectly and all apertures are closed.
2. The usage of a Class 3B laser is only permitted under the control of a laser safety officer.
3. A Class 3B laser can cause irreversible damage to your eyes. Thus do not stare into the
laser beam or illuminated fiber. Make sure to wear adequate eye protection during operation
of the laser in maintenance mode.
4. Always operate the
training in operating the system in maintenance mode. Inform your laser safety officer if you
intend to operate the system in maintenance mode. The user mode is the safest manner to
operate the system. Opening the stage cover or the binocular slider will interrupt the laser
beam in this mode.
5. Only trained Olympus service personnel is allowed to perform the installation and alignment
procedures described in the Service Manual.
6. A person in charge with the laser alignment or performing scientific experiments while oper-
ating the laser in maintenance mode must be aware of the safety precautions adequate to
the TIRF microscope setup. He/she is responsible for the safety of any person present in the
room while operating the lasers in maintenance mode.
illuminator system in user mode unless you underwent a detailed
illuminator system check if all devices are attached cor-
7. The focused laser beam or any UV light emitted from the microscope nosepiece may injure
the skin through the objective exit lens and through a free nosepiece position. Thus, avoid
exposing the hands to the laser beam when working on the microscope.
8. Do not bend, step on or pull excessively on the laser fibers. This could damage a fiber and
cause hazardous beam leakage. In case of a damaged fiber, switch off the laser immediately
and contact Olympus.
9. Be sure all apertures of your microscope system stay closed or are connected while operat-
ing the laser. A change in camera or any microscope part should be performed only when
the laser is turned off.
10. Never cover the air outlet of the laser to prevent overheating.
Illumination Combiner, User Manual Chapter 3 – Safety Precautions and Handling Instructions 11
Direct exposure to the laser light is possible at the following positions:
• The laser system’s housing at the fiber port if the fiber is removed
• The exit of the laser fiber
• The fiber coupling of the widefield illumination system to the
• The beam path of the microscope
• Any microscope port, which includes the entrance ports because the laser light can be reflected
within the microscope
• The light output port of the widefield illumination system
• The objective tip or – if no objective is mounted – a free nose piece position
illuminator system
3.2 General Safety Precautions
a Always use power cords and power supplies provided or approved by Olympus Soft Imaging Solu-
tions or the manufacturer of your laser system.
b Provide unimpaired access to the emergency shutter switch at the LAS-MC manual control and to
the main power switches.
c Only those lasers may be connected that comply the laser safety regulations as described in Chap-
ter 1.2.
d Always make sure that the grounding terminal of the laser system and the wall outlet are connected
properly. If the system is not grounded, Olympus Soft Imaging Solutions cannot warrant the electrical safety and proper performance of the product.
e Please refer to the user manuals of the respective laser manufacturer for adequate safety precau-
tions and proper usage.
f If the equipment is not used as specified in this manual, the safety and performance may be im-
paired. In addition the equipment may be damaged and warranty may be lost. Use the equipment
only as outlined in this instruction manual.
Olympus Soft Imaging Solutions GmbH accepts no liability for any health damages caused
by improper use of the lasers and laser related setup!
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