OLYMPUS CELL FRAP User Manual [ru]

®

I

INSTRUCTIONS

SOFTWARE MODULE AND

LASER SAFETY FOR THE HIGH-SPEED SCANNING FRAP SYSTEM

This instruction manual describes the configuration and usage of the scanning FRAP system within the Olympus Imaging Stations. To ensure safety, obtain optimum performance and familiarize yourself fully with the use of the product, we recommend that you study this manual thoroughly before operation. Together with this manual, please also read the hardware manual, the hardware and software manuals as well as the instruction manuals of the microscope in order to understand overall operation methods. Retain this manual in an easily accessible place near a system for future reference.

OLYMPUS SOFT IMAGING SOLUTIONS GMBH

Johann-Krane-Weg 39 D-48149 Münster

Tel: +49 89 - 89 55 805 660

Fax +49 89 - 89 55 805 6606 Email: [e-mail]

www.olympus-sis.com

III

Imaging Excellence

We at Olympus Soft Imaging Solutions GmbH have tried to make the information in this manual as accurate and reliable as possible. Nevertheless, Olympus Soft Imaging Solutions GmbH disclaims any warranty of any kind, whether expressed or implied, as to any matter whatsoever relating to this manual, including without limitation the merchantability or fitness for any particular purpose. Olympus Soft Imaging Solutions GmbH will from time to time revise the software described in this manual and reserves the right to make such changes without obligation to notify the purchaser. In no event shall Olympus Soft Imaging Solutions GmbH be liable for any indirect, special, incidental, or consequential damages arising out of purchase or use of this manual or the information contained therein.

No part of this document may be reproduced or transmitted in any form or by any means, electronic or mechanical, for any purpose, without the prior permission of Olympus Soft Imaging Solutions GmbH.

2010 by Olympus Soft Imaging Solutions GmbH. All rights reserved.

Manual version: September 2010

Manual

Contents

Contents

1	Introduction.........................................................................................................			1
1.1		Abstract ........................................................................................................		2
1.2		General .........................................................................................................		3
1.3		Components .................................................................................................		3
2	Safety Symbols ...................................................................................................			5
3	Safety Precautions and Handling Instructions ...............................................			9
3.1		Laser Safety Precautions............................................................................		10
3.2		General Safety Precautions ........................................................................		11
4		Overview and Laser Safety Setup ...................................................		13
4.1		Setup Scheme ............................................................................................		14
4.2		The Laser Safety Features – Overview and Check.....................................		15
4.3		Mounting the Laser Safety Kit ....................................................................		17
4.4		Connecting the	Control Unit .......................................................	18
4.5		Connecting the LAS-BOB Laser Safety Break-out Box .............................		19
4.6		The Laser Shutter Manual Control..............................................................		20
4.7		Key Control .................................................................................................		21

4.8The Remote Interlock Connector of the Olympus Soft Imaging Solutions

	ODB Laser Systems	....................................................................................	22
5 Modes of Operation..........................................................................................			23
5.1	The User Mode ...........................................................................................		24
5.2	The Maintenance Mode ..............................................................................		25
5.2.1	Exchanging Microscope ...........................................or Camera Hardware		25
5.2.2	Access to the Specimen ..............without Deactivating the FRAP System		26
5.2.3	Observing the Specimen ............................................Through the Ocular		26
6 Operating the .......................................................................		System	29
6.1	System Configuration .................................................................................		30
6.2	The FRAP GUI in ...............................................................		31
6.3	in the Experiment ........................................................Manager		33
6.3.1	FRAP Experiments......................................................................................		33
6.3.2	Fire On Click ...............................................................................................		36
6.3.3	Pattern Bleaching – Fluorescence ............................Speckle Microscopy		37
7 Appendix ..........................................................................................................			39
7.1	Laser Safety Stage Cover .......................................................Dimensions		40

Contents

Manual

Chapter 1 –Introduction

1

1 Introduction

Thank you very much for purchasing Olympus Soft Imaging Solutions' state of art scanning FRAP system and for your confidence in our products and service. It is Olympus Soft Imaging Solutions' main objective to provide you with solutions able to meet your experimental demands and thus pave the way to your scientific success.

The scanning FRAP system is designed for general laboratory use only. It allows coupling up to two lasers to an inverted Olympus IX2 FRAP microscope.

1.1	Abstract ........................................................................................................	2
1.2	General .........................................................................................................	3
1.3	Components .................................................................................................	3

2Chapter 1 – Introduction

scan head mounted on an IX81

1.1 Abstract

In the following sections you will find a detailed description of the components of scanning FRAP system, handling and alignment instructions and how to maintain the performance of your FRAP microscope. Keep in mind that all components are designed to work together with an Olympus Imaging Station as an integrated system. We strongly discourage taking away or replacing single components without prior consultation of Olympus Soft Imaging Solutions, most probably it will impair the performance of the system dramatically. The guarantee will not cover any damages to the system due to such mishandling. All necessary tools for handling and alignment (except the laser safety goggles) are part of the product.

If you find any information missing in this manual or you need additional support, please contact your local Olympus representative.

Manual

Chapter 1 –Introduction

3

1.2 General

The MITICO motorized integrated TIRF illumination combiner is compatible with the Olympus microscope frames IX81S1F-FRAP, IX81S1F-FRAP-ZDC, and IX71S1F-FRAP, as well as a range of diode laser systems between 405 nm and 700 nm provided that they meet the following requirements:

•Every laser unit must have an input remote-control safety interlock that must be connected to the central laser emergency stop and which interrupts the laser radiation in case of danger (EN 60825- 1:2003, Sect. 4.4 and 10.2). Furthermore, every laser unit must have a key-activated main power switch in the sense of EN 60825-1:2003, Sect. 4.5).

•Provided that the laser units can be operated independently they must have their own emission warning display (audible or visible) (EN 60825-1:2003, Sect. 4.6).

•The combined power of all employed and/or connected laser powers must not exceed 500 mW.

This instructions manual describes the hardware components of the scanning FRAP system, and their installation. It also explains the software configuration and the use o the system.

It is important to familiarize yourself with the entire system to ensure safety and optimum performance. We recommend studying this manual as well as the software and hardware manuals of the imaging system thoroughly before operating the scanning FRAP system. Keep this manual close to the imaging station for future reference.

The scanning FRAP system meets the CE requirements:

•EN61326 Class A

•EN60825

1.3 Components

The scanning FRAP system consists of the following components:

aLaser shutter hand switch LAS-MC with key with emergency interrupter and key-controlled switch to maintenance mode

b Laser safety break-out box LAS-BOB

c Laser safety stage cover with translucent laser safety glass that allows following the beam position

d Laser safety ocular shutter

e Manual

The software module for the imaging software is likewise required as well as at least one laser, favorably a dedicated OSIS FRAP diode laser:

Furthermore the following scanner head and controller shall be used (Rapp Optoelectronic):

4Chapter 1 – Introduction

a	illuminator featuring two fiber ports, each with adjustable line focus

b control unit

Manual

Chapter 2 – Safety Symbols 5

2 Safety Symbols

The deactivation of the safety systems or the dismantling of different components by the operator is required in certain operational modes and in case of illumination alignment. As a result the Olympus scanning FRAP system is to be classified in accordance with EN 60825-1:2003 in laser safety class 3B and in accordance to EN 954-1:1997 in hazard category 2. Class 3B lasers are medium power lasers with an output power of up to 500 mW within 400 – 700 nm wavelength. Laser safety regulations require extensive labeling of all possible apertures where laser radiation might potentially exit including such apertures that are totally covered except upon partially or totally disassembling the system.

6 Chapter 2 – Safety Symbols

The following symbols are placed on the Olympus scanning FRAP system. Study the meaning of the symbols and always use the equipment in the safest possible manner.

General symbols

Symbol	Explanation
	Indicates the use of a laser beam. Take special care in handling that part.
	(See also following chapter)
	Carefully read the instructions on the label and the manual before use. Improper use could
	result in personal injury to the user and/or damage the equipment.
	This symbol indicates separate collection of waste electrical and electronic equipment in the EU
	countries.
	Do not throw the equipment into the domestic refuse
	Please use the return and collection systems available in your country for the disposal of this
	product

Each laser source must be provided with a danger symbol, a sign with the classification text and a sign on the beam exit opening. Please ensure that your microscope is labeled as shown in the table below. For all parts labeled with the following labels special laser precautions must be considered (see Chapter 4.1, Laser Safety Precautions)!

Laser safety symbols

Symbol	Explanation	Location
	Indicates the use of a laser beam.	scan head
	Take special care in handling this part.
	Laser radiation can cause irreversible
	damage to your eyes. Wear adequate	Microscope frame, right side, left side
	protection before opening this	near C-mount; see photos 2 and 3.
	aperture.
	These apertures should be closed
	while operating the system. Parts
	connected should be dismounted by	Laser safety ocular shutter; see photo 4.
	trained personal only and when the
	laser is turned off.
	When system is switched to
	maintenance mode laser radiation can	Laser safety stage cover; see photo 5.
	be emitted from this aperture. Be sure
	to wear adequate eye protection.

Labels for the microscope components are included in the shipping list of the Olympus scanning FRAP system. Please paste them on the microscope upon system installation. If any label is missing please ask your Olympus representative for additional labels.

Manual

Chapter 2 – Safety Symbols 7

1: scan head

2: microscope frame, right side

3: fluorescence turret and C-mount

4: laser safety ocular shutter

5: laser safety stage cover

8Chapter 2 – Safety Symbols

Illumination Combiner, User Manual

Chapter 3 – Safety Precautions and Handling Instructions

9

3Safety Precautions and Handling Instructions

The Olympus scanning FRAP system must be used with laser products belonging to the Laser Safety Class 3B. Class 3B lasers are medium power lasers with an output power of up to 500 mW within 400 – 700 nm wavelength. The total of the output power of the connected lasers may not exceed 500 mW. Viewing these lasers under direct beam and specular reflection conditions is hazardous. The diffuse reflection is usually not a hazard except for higher power Class 3B and Class 4 lasers. Normally the class 3B laser is not a fire hazard. A class 3B laser product may only be used under the control of a laser safety officer. Before using this product be sure to be familiar with the demanded safety measures according to EN 60825-1 and the national regulations

3.1	Laser Safety Precautions............................................................................	10
3.2	General Safety Precautions ........................................................................	11

10 Chapter 3 – Safety Precautions and Handling Instructions

3.1 Laser Safety Precautions

Caution: Control or adjustments using procedures other than those prescribed in this manual will lead to hazardous laser beam exposure.

To avoid hazardous situations strictly follow the laser safety instructions below.

1.Before operating an Olympus scanning FRAP system check if all devices are attached correctly and all apertures are closed.

2.The usage of a Class 3B laser is only permitted under the control of a laser safety officer.

3.A Class 3B laser can cause irreversible damage to your eyes. Thus do not stare into the laser beam or illuminated fiber. Make sure to wear adequate eye protection during operation of the laser in maintenance mode.

4.Always operate the scanning FRAP system in user mode unless you underwent a detailed training in operating the system in maintenance mode. Inform your laser safety officer if you intend to operate the system in maintenance mode. The user mode is the safest manner to operate the system. Opening the stage cover or the binocular slider will interrupt the laser beam in this mode.

5.Only trained Olympus service personnel is allowed to perform the installation and alignment procedures described in the Service Manual.

6.A person in charge with the laser alignment or performing scientific experiments while operating the laser in maintenance mode must be aware of the safety precautions adequate to the TIRF microscope setup. He/she is responsible for the safety of any person present in the room while operating the lasers in maintenance mode.

7.The focused laser beam or any UV light emitted from the microscope nosepiece may injure the skin through the objective exit lens and through a free nosepiece position. Thus, avoid exposing the hands to the laser beam when working on the microscope.

8.Do not bend, step on or pull excessively on the laser fibers. This could damage a fiber and cause hazardous beam leakage. In case of a damaged fiber, switch off the laser immediately and contact Olympus.

9.Be sure all apertures of your microscope system stay closed or are connected while operating the laser. A change in camera or any microscope part should be performed only when the laser is turned off.

10.Never cover the air outlet of the laser to prevent overheating.