OLYMPUS CELL FRAP User Manual [ru]
®
I
INSTRUCTIONS
SOFTWARE MODULE AND
LASER SAFETY FOR THE
HIGH-SPEED SCANNING FRAP SYSTEM
This instruction manual describes the configuration and usage of the scanning FRAP
system within the Olympus
performance and familiarize yourself fully with the use of the product, we recommend that you
study this manual thoroughly before operation. Together with this manual, please also read the
hardware manual, the hardware and software manuals as well as the
instruction manuals of the microscope in order to understand overall operation methods. Retain
this manual in an easily accessible place near a system for future reference.
Imaging Stations. To ensure safety, obtain optimum
OLYMPUS SOFT IMAGING SOLUTIONS GMBH
Johann-Krane-Weg 39
D-48149 Münster
Tel: +49 89 - 89 55 805 660
Fax +49 89 - 89 55 805 6606
Email: info@olympus-sis.com
www.olympus-sis.com
III
Imaging Excellence
We at Olympus Soft Imaging Solutions GmbH have tried to make the information in this manual as
accurate and reliable as possible. Nevertheless, Olympus Soft Imaging Solutions GmbH disclaims any
warranty of any kind, whether expressed or implied, as to any matter whatsoever relating to this
manual, including without limitation the merchantability or fitness for any particular purpose. Olympus
Soft Imaging Solutions GmbH will from time to time revise the software described in this manual and
reserves the right to make such changes without obligation to notify the purchaser. In no event shall
Olympus Soft Imaging Solutions GmbH be liable for any indirect, special, incidental, or consequential
damages arising out of purchase or use of this manual or the information contained therein.
No part of this document may be reproduced or transmitted in any form or by any means, electronic or
mechanical, for any purpose, without the prior permission of Olympus Soft Imaging Solutions GmbH.
2010 by Olympus Soft Imaging Solutions GmbH. All rights reserved.
Manual version: September 2010
Manual Contents
Contents
1 Introduction......................................................................................................... 1
1.1 Abstract ........................................................................................................ 2
1.2 General ......................................................................................................... 3
1.3 Components .................................................................................................3
2 Safety Symbols ...................................................................................................5
3 Safety Precautions and Handling Instructions ...............................................9
3.1 Laser Safety Precautions ............................................................................10
3.2 General Safety Precautions ........................................................................11
4 Overview and Laser Safety Setup ................................................... 13
4.1 Setup Scheme ............................................................................................14
4.2 The Laser Safety Features – Overview and Check .....................................15
4.3 Mounting the Laser Safety Kit .................................................................... 17
4.4 Connecting the Control Unit ....................................................... 18
4.5 Connecting the LAS-BOB Laser Safety Break-out Box .............................19
4.6 The Laser Shutter Manual Control.............................................................. 20
4.7 Key Control ................................................................................................. 21
4.8 The Remote Interlock Connector of the Olympus Soft Imaging Solutions
ODB Laser Systems....................................................................................22
5 Modes of Operation.......................................................................................... 23
5.1 The User Mode ...........................................................................................24
5.2 The Maintenance Mode .............................................................................. 25
5.2.1 Exchanging Microscope or Camera Hardware...........................................25
5.2.2 Access to the Specimen without Deactivating the FRAP System .............. 26
5.2.3 Observing the Specimen Through the Ocular ............................................26
6 Operating the System.......................................................................29
6.1 System Configuration .................................................................................30
6.2 The FRAP GUI in ............................................................... 31
6.3
6.3.1 FRAP Experiments...................................................................................... 33
6.3.2 Fire On Click ...............................................................................................36
6.3.3 Pattern Bleaching – Fluorescence Speckle Microscopy ............................37
7 Appendix ..........................................................................................................39
7.1 Laser Safety Stage Cover Dimensions .......................................................40
in the Experiment Manager ........................................................ 33
Contents
Manual Chapter 1 –Introduction 1
1 Introduction
Thank you very much for purchasing Olympus Soft Imaging Solutions' state of art
FRAP system and for your confidence in our products and service. It is Olympus Soft Imaging
Solutions' main objective to provide you with solutions able to meet your experimental demands and
thus pave the way to your scientific success.
The
two lasers to an inverted Olympus IX2 FRAP microscope.
scanning FRAP system is designed for general laboratory use only. It allows coupling up to
scanning
1.1 Abstract ........................................................................................................ 2
1.2 General ......................................................................................................... 3
1.3 Components .................................................................................................3
2 Chapter 1 – Introduction
scan head mounted on an IX81
1.1 Abstract
In the following sections you will find a detailed description of the components of
FRAP system, handling and alignment instructions and how to maintain the performance of your FRAP
microscope. Keep in mind that all components are designed to work together with an Olympus
Imaging Station as an integrated system. We strongly discourage taking away or replacing
single components without prior consultation of Olympus Soft Imaging Solutions, most probably it will
impair the performance of the system dramatically. The guarantee will not cover any damages to the
system due to such mishandling. All necessary tools for handling and alignment (except the laser safety
goggles) are part of the product.
If you find any information missing in this manual or you need additional support, please contact your
local Olympus representative.
scanning
Manual Chapter 1 –Introduction 3
1.2 General
The MITICO motorized integrated TIRF illumination combiner is compatible with the Olympus
microscope frames IX81S1F-FRAP, IX81S1F-FRAP-ZDC, and IX71S1F-FRAP, as well as a range of
diode laser systems between 405 nm and 700 nm provided that they meet the following requirements:
• Every laser unit must have an input remote-control safety interlock that must be connected to the
central laser emergency stop and which interrupts the laser radiation in case of danger (EN 608251:2003, Sect. 4.4 and 10.2). Furthermore, every laser unit must have a key-activated main power
switch in the sense of EN 60825-1:2003, Sect. 4.5).
• Provided that the laser units can be operated independently they must have their own emission
warning display (audible or visible) (EN 60825-1:2003, Sect. 4.6).
• The combined power of all employed and/or connected laser powers must not exceed 500 mW.
This instructions manual describes the hardware components of the scanning FRAP system,
and their installation. It also explains the software configuration and the use o the system.
It is important to familiarize yourself with the entire system to ensure safety and optimum performance.
We recommend studying this manual as well as the software and hardware manuals of the imaging
system thoroughly before operating the scanning FRAP system. Keep this manual close to the
imaging station for future reference.
The scanning FRAP system meets the CE requirements:
• EN61326 Class A
• EN60825
1.3 Components
The scanning FRAP system consists of the following components:
a Laser shutter hand switch LAS-MC with key with emergency interrupter and key-controlled switch
to maintenance mode
b Laser safety break-out box LAS-BOB
c Laser safety stage cover with translucent laser safety glass that allows following the beam position
d Laser safety ocular shutter
e Manual
The software module for the imaging software is likewise required as well as at least
one laser, favorably a dedicated OSIS FRAP diode laser:
Furthermore the following scanner head and controller shall be used (Rapp Optoelectronic):
4 Chapter 1 – Introduction
a illuminator featuring two fiber ports, each with adjustable line focus
b control unit
Manual Chapter 2 –
Safety Symbols
5
2 Safety Symbols
The deactivation of the safety systems or the dismantling of different components by the operator is
required in certain operational modes and in case of illumination alignment. As a result the Olympus
scanning FRAP system is to be classified in accordance with EN 60825-1:2003 in laser safety
class 3B and in accordance to EN 954-1:1997 in hazard category 2. Class 3B lasers are medium
power lasers with an output power of up to 500 mW within 400 – 700 nm wavelength. Laser safety
regulations require extensive labeling of all possible apertures where laser radiation might potentially
exit including such apertures that are totally covered except upon partially or totally disassembling the
system.
6 Chapter 2 –
Safety Symbols
The following symbols are placed on the Olympus scanning FRAP system. Study the meaning
of the symbols and always use the equipment in the safest possible manner.
General symbols
Symbol Explanation
Indicates the use of a laser beam. Take special care in handling that part.
(See also following chapter)
Carefully read the instructions on the label and the manual before use. Improper use could
result in personal injury to the user and/or damage the equipment.
This symbol indicates separate collection of waste electrical and electronic equipment in the EU
countries.
Do not throw the equipment into the domestic refuse
Please use the return and collection systems available in your country for the disposal of this
product
Each laser source must be provided with a danger symbol, a sign with the classification text and a sign
on the beam exit opening. Please ensure that your microscope is labeled as shown in the table below.
For all parts labeled with the following labels special laser precautions must be considered (see
Chapter 4.1, Laser Safety Precautions)!
Laser safety symbols
Symbol Explanation Location
Indicates the use of a laser beam.
Take special care in handling this part.
Laser radiation can cause irreversible
damage to your eyes. Wear adequate
protection before opening this
aperture.
These apertures should be closed
while operating the system. Parts
connected should be dismounted by
trained personal only and when the
laser is turned off.
When system is switched to
maintenance mode laser radiation can
be emitted from this aperture. Be sure
to wear adequate eye protection.
scan head
Microscope frame, right side, left side
near C-mount; see photos 2 and 3.
Laser safety ocular shutter; see photo 4.
Laser safety stage cover; see photo 5.
Labels for the microscope components are included in the shipping list of the Olympus
scanning FRAP system. Please paste them on the microscope upon system installation. If any label is
missing please ask your Olympus representative for additional labels.
Manual Chapter 2 –
Safety Symbols
7
1:
scan head
2: microscope frame, right side 3: fluorescence turret and C-mount
4: laser safety ocular shutter 5: laser safety stage cover
8 Chapter 2 –
Safety Symbols
Illumination Combiner, User Manual Chapter 3 – Safety Precautions and Handling Instructions 9
3 Safety Precautions and Handling
Instructions
The Olympus scanning FRAP system must be used with laser products belonging to the Laser
Safety Class 3B. Class 3B lasers are medium power lasers with an output power of up to 500 mW
within 400 – 700 nm wavelength. The total of the output power of the connected lasers may not exceed
500 mW. Viewing these lasers under direct beam and specular reflection conditions is hazardous. The
diffuse reflection is usually not a hazard except for higher power Class 3B and Class 4 lasers. Normally
the class 3B laser is not a fire hazard. A class 3B laser product may only be used under the control of a
laser safety officer. Before using this product be sure to be familiar with the demanded safety measures
according to EN 60825-1 and the national regulations
3.1 Laser Safety Precautions ............................................................................10
3.2 General Safety Precautions ........................................................................11
10 Chapter 3 – Safety Precautions and Handling Instructions
3.1 Laser Safety Precautions
Caution: Control or adjustments using procedures other than those prescribed in this
manual will lead to hazardous laser beam exposure.
To avoid hazardous situations strictly follow the laser safety instructions below.
1. Before operating an Olympus scanning FRAP system check if all devices are
attached correctly and all apertures are closed.
2. The usage of a Class 3B laser is only permitted under the control of a laser safety officer.
3. A Class 3B laser can cause irreversible damage to your eyes. Thus do not stare into the
laser beam or illuminated fiber. Make sure to wear adequate eye protection during operation
of the laser in maintenance mode.
4. Always operate the scanning FRAP system in user mode unless you underwent a
detailed training in operating the system in maintenance mode. Inform your laser safety
officer if you intend to operate the system in maintenance mode. The user mode is the
safest manner to operate the system. Opening the stage cover or the binocular slider will
interrupt the laser beam in this mode.
5. Only trained Olympus service personnel is allowed to perform the installation and alignment
procedures described in the Service Manual.
6. A person in charge with the laser alignment or performing scientific experiments while
operating the laser in maintenance mode must be aware of the safety precautions adequate
to the TIRF microscope setup. He/she is responsible for the safety of any person present in
the room while operating the lasers in maintenance mode.
7. The focused laser beam or any UV light emitted from the microscope nosepiece may injure
the skin through the objective exit lens and through a free nosepiece position. Thus, avoid
exposing the hands to the laser beam when working on the microscope.
8. Do not bend, step on or pull excessively on the laser fibers. This could damage a fiber and
cause hazardous beam leakage. In case of a damaged fiber, switch off the laser immediately
and contact Olympus.
9. Be sure all apertures of your microscope system stay closed or are connected while
operating the laser. A change in camera or any microscope part should be performed only
when the laser is turned off.
10. Never cover the air outlet of the laser to prevent overheating.
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