Leica SP2600 User Manual
Supplementary instruction manual Leica SP2600
To be used only in conjunction
with instruction manual Leica SM2500 .
V1.2 English 07/2002
Always keep this manual near the instrument.
Read carefully prior to operating the instrument.
Leica SP2600
Ultramilling attachment
NOTE
The information, numerical data, notes and value
judgments contained in this manual represent the
current state of scientific knowledge and stateof-the-art technology as we understand it following thorough investigation in this field.
We are under no obligation to update the present
manual according to the latest technical developments, nor to provide our customers with additional copies, updates etc. of this manual.
For erroneous statements, drawings, technical
illustrations etc. contained in this manual we exclude liability as far as permissible according to
the national legal system applicable in each individual case. In particular, no liability whatsoever
is accepted for any financial loss or consequential damage caused by or related to compliance
with statements or other information in this manual.
Statements, drawings, illustrations and other information as regards contents or technical details of the present manual are not to be considered as warranted characteristics of our
products.
These are determined only by the contract provisions agreed between ourselves and our customers.
Leica reserves the right to change technical
specifications as well as manufacturing processes without prior notice. Only in this way is it possible to continuously improve the technology and
manufacturing techniques used in our products.
This document is protected under copyright laws.
Any copyrights of this document are retained by
Leica Microsystems Nussloch GmbH.
Any reproduction of text and illustrations (or of
any parts thereof) by means of print, photocopy,
microfiche, web cam or other methods including any electronic systems and media requires
express prior permission in writing by Leica Microsystems Nussloch GmbH.
For the instrument serial number and year of
manufacture, please refer to the name plate at
the back of the instrument.
© Leica Microsystems Nussloch GmbH
Published by:
Leica Microsystems Nussloch GmbH
Heidelberger Str. 17 - 19
D-69226 Nussloch
Germany
Telephone: +49 (0)6224 143-0
Facsimile: +49 (0)6224 143-200
Internet: http://www.histo-solutions.com
Leica SP2600 Ultramilling attachment
3
Table of contents
1. Important information................................................................................................................6
1.1 Symbols in the text and their meaning ................................................................................................ 6
1.2 Designated use........................................................................................................................................ 6
1.3 Information on this instruction manual................................................................................................ 7
2. Safety ............................................................................................................................................ 8
2.1 Safety regulations ................................................................................................................................... 8
2.2 Built-in safety devices............................................................................................................................ 9
2.3 Safety instructions ................................................................................................................................ 10
3. Technical Data ......................................................................................................................... 13
4. Installation................................................................................................................................. 14
4.1 Overview - Leica SP2600 Ultramilling attachment / basic instrument Leica SM2500
- instrument parts / functions .............................................................................................................. 14
4.2 Leica SP2600 standard delivery.......................................................................................................... 15
4.3 Site requirements.................................................................................................................................. 15
4.4 Unpacking, transport, installation ...................................................................................................... 15
4.5 Installing the Leica SP2600 ultramilling attachment........................................................................ 16
4.6 Connecting the vacuum cleaner......................................................................................................... 17
4.7 Installing additional accessories........................................................................................................ 18
5. Operation ..................................................................................................................................... 19
5.1 Overview of chapter 5 Operation...................................................................................................... 19
5.2 Adjusting the pre-millers in the calibrating device..........................................................................20
5.3 Adjusting the finishing millers in the calibrating device ................................................................. 25
5.4 Specimen holder systems - how to combine individual components .......................................... 29
5.5 Clamping specimen .............................................................................................................................. 30
5.5.1 Installing the base plates in the basic instrument ........................................................................... 30
5.5.2 Clamping the specimen holders in the base plate with dove tail guide ....................................... 30
5.5.3 Clamping the vacuum stages on/in the base plates........................................................................ 31
5.5.4 Connecting the vacuum pump / placing specimens onto the vacuum stages ............................ 32
5.6 Preparatory steps prior to the milling procedure ............................................................................ 34
5.7 Pre-milling .............................................................................................................................................. 36
5.7.1 Perspective view of the pre-milling procedure................................................................................ 38
5.8 Finishing milling ..................................................................................................................................... 40
5.8.1 Perspective view of the finishing milling procedure ....................................................................... 42
5.9 Switching the instrument off (switching off the control unit .......................................................... 43
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Supplementary instruction manual V 1.2 07/2002
Important information
6. Troubleshooting ....................................................................................................................... 44
7. Cleaning, disinfection and maintenance ............................................................................ 45
7.1 Cleaning and disinfection .................................................................................................................... 45
7.2 Maintenance.......................................................................................................................................... 46
7.2.1 Resharpening the millers ..................................................................................................................... 46
7.2.2 Replacing the light bulb in the calibration device............................................................................ 47
8. Overview - accessories .......................................................................................................... 48
8.1 Miller holders and millers .................................................................................................................... 48
8.2 Base plates for specimen holders and vacuum stage adapters ................................................... 48
8.3 Specimen holders ................................................................................................................................. 48
8.4 Vacuum stage adapters ....................................................................................................................... 48
8.5 Vacuum stages and matching lids / perforated specimen holders .............................................. 49
8.6 Vacuum pumps ...................................................................................................................................... 49
8.7 Additional accessories ........................................................................................................................ 50
9. Warranty and service ................................................................................................................ 51
Leica SP 2600 Ultramilling attachment
5
1. Important information
1.1 Symbols in the text and their meaning
Warnings and cautions appear in a
grey box and are marked by a warning
triangle.
Failure to comply with these warnings/
cautions can lead to accidents, bodily
injury and damage to instrument and/
or accessories.
Notes, i.e. important information for the
user, appear in a grey box and are
marked by an information sign.
Figures in brackets refer to item num-
(5)
bers in illustrations or to the illustrations themselves.
1.2 Designated use
Fig. 1.1
The Leica SP2600 is designated to prepare milled
surfaces and thin slices of specimen materials
such as plastics and metals as well as of organic
materials (such as bones, teeth) in medicine, biology and industry.
The Leica SP2600 ultramilling attachment is an
accessory which cannot be operated as a standalone device but must be used in conjunction
with the Leica SM2500 sliding microtome described in a separate instruction manual.
ENTER
6
Function keys that need to be pressed
Fig. 5.23
on the control unit are printed in bold
and capital letters.
Specimen surfaces are milled with a rotating
spindle, equipped with either a pre- or a finishing
miller.
With each sledge stroke, the micrometer feed
mechanism of the Leica SM2500 lowers the miller towards the specimen by the feed setting selected.
The Leica SP2600 may be used only in conjunction with accessories and supplementary instruments made by Leica.
If the instrument is used for any other than
the designated application, this will be
considered an improper use of the product!
Supplementary instruction manual V 1.2 07/2002
1.3 Information on this instruction manual
Instrument type
This supplementary instruction manual must be used together with the Leica
SM2500 sliding microtome instruction manual describing the basic instrument Leica SM2500, which is needed to operate the Leica SP2600 ultramilling attachment.
All information provided in this supplementary instruction manual applies
only to the instrument type indicated on the title page.
A name plate indicating the instrument type and serial number is attached to
the rear of the Leica SP2600 ultramilling device.
Fig. 1.2
Working with this instruction manual
This supplementary instruction manual contains important instructions and
information related to the operating safety and maintenance of the Leica
SP2600 ultramilling attachment.
It is an important part of the Leica SP2600 ultramilling attachment.
Prior to setting up and operating the Leica SP2600 together with the Leica
SM2500 be sure to read carefully both this supplementary instruction
manual as well as the Leica SM2500 instruction manual, with the exception
of chapters 5.5.6 - 5.7 of the Leica SM2500 manual. These chapters describe
installation of knife holders / knives and sectioning with the Leica SM2500
and are not of importance for operating the Leica SP2600 ultramilling attachment.
1. Important information
Leica SP 2600 Ultramilling attachment
If additional requirements on accident prevention and environmental protection exist in the country of operation, this supplementary
instruction manual must be supplemented by additional appropriate instructions to ensure compliance with such requirements.
7
2. Safety
2.1 Safety regulations
This instrument has been built and tested in accordance with the same directives and standards as the Leica SM2500.
For a complete list of the directives and standards applied please refer to
chapter 2.1, page 8 of the Leica SM2500 instruction manual.
In order to maintain the SP2600 in a condition compliant with the above directives and standards and to ensure safe operation, the operator must observe the instructions and warnings contained in this supplementary instruction manual as well as in the Leica SM2500 instruction manual.
The Leica SP2600 ultramilling may be operated only in conjunction
with the Leica SM2500 sliding microtome!
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Supplementary instruction manual V 1.2 07/2002
2.2 Built-in safety devices
The instrument is equipped with the following safety devices:
1
Plexiglass protective cover
The miller is equipped with a protective cover made of plexiglass (1).
When the cover is moved to the upper limit position (1, Fig. 2.1) the miller
is automatically blocked and cannot be started.
For any manipulations in the milling area (such as inserting millers, fine
adjustment of specimen height etc.) the protective cover (1, Fig. 2.1) must
be in the upper limit position.
Fig. 2.1
1
To be able to start the miller, the protective cover must be lowered (1,
Fig. 2.2).
2. Safety
Never touch the milling spindle while the plexiglass cover is shut,
no matter whether the spindle is rotating or not.
Fig. 2.2
Important note on the safety devices built in by the manufacturer:
The safety devices provided by the manufacturer constitute only a basis for
accident prevention.
Major responsibility for accident prevention during the use of the instrument
rests with the employer who has the operating authority for the instrument
and, in addition, with the persons, designated by him, who operate, service
and repair the instrument.
To ensure safe and trouble-free operation of the instrument, be sure to
comply with all instructions, warning and cautions in this supplementary
instruction manual as well as in the Leica SM2500 instruction manual!
Leica SP 2600 Ultramilling attachment
9
2. Safety
2.3 Safety instructions
Be sure to read and comply with the
safety instructions, warnings and cautions in this chapter as well as in
chapter 2 of the Leica SM2500 instruction manual, even if you are already familiar with the operation and use of
other Leica products.
Safety instructions - general
The Leica SP2600 ultramilling attachment may only be operated in conjunction with the Leica SM2500 sliding microtome.
Prior to working with the Leica SP2600
ultramilling attachment, be sure to
read the following instruction manuals:
The entire Leica SM2500 instruction
manual except chapters 5.5.6
through 5.7.
This supplementary instruction
manual Leica SP2600.
The Leica SP2600 ultramilling attachment may only be operated by skilled
laboratory personnel. It may only be
used for the designated application
and operated in accordance with the
instructions contained in this supplementary instruction manual and in the
Leica SM2500 instruction manual!
Safety instructions - transport and installation
Be sure to follow the unpacking instructions (attached to the outside of
the instrument shipping crate)!
Never remove or modify any of the
safety devices installed on the instrument and/or accessories!
Before mounting the ultramilling attachment, make sure the bearing
blocks of the microtome supporting the
milling attachment and the bearing
surfaces of the milling attachment are
clean.
Two people are required to place the
milling attachment on the bearing
blocks from above.
Do not slide the milling attachment
onto the bearing blocks to prevent the
bearing surfaces of the microtome and
milling attachment from getting
scratched!
The bearing surfaces must not become
damaged under any circumstances!
Caution:
When releasing the lid of the milling
attachment (can be lifted and used as
a handle) be careful not to get your fingers caught between the lid and the
body of the milling attachment!
10
Supplementary instruction manual V 1.2 07/2002
2. Safety
Safety instructions - transport and installation
Caution!
Only the vacuum cleaner offered by
Leica or other vacuum cleaner brands
rated at 230 V/ 50 Hz and a maximum
power draw of 4 A can be connected
directly to the SM2500 control unit (see
page 17), and this only in countries
where the mains supply is 230 V / 50
Hz!
Otherwise, the vacuum cleaner must
be connected directly to mains and operated via the on/off switch of the
vacuum cleaner.
Safety instructions - inserting the millers into the
holders / working with the calibrating device
Twisted or deformed springs for the
miller holders must be exchanged!
When the millers are inserted into the
holders the letter V (pre-millers) or F
(finishing miller) must be visible for the
milling edge to point into the right direction.
Caution:
The gauge contact plate must never hit
the milling edge.
This could damage the surface of the
contact plate, leading to incorrect
measuring results.
Be very careful when drawing the contact plate close to the milling edge.
Always use the magnifier.
Safety instructions working with the instrument
Never remove or modify any of the
safety devices installed on the instrument and/or accessories!
Never touch the milling spindle while
the plexiglass cover is shut, no matter
whether the spindle is rotating or not.
Always slide the plexiglass cover to
the upper position prior to attaching
the miller holders to the milling
spindle!
Wear protective gloves when working
with potentially infectious material!
Always slide the plexiglass cover to
the upper position prior to checking
the distance between milling edge
and specimen surface! - Risk of injury!
Always check the distance between
miller cutting edge and specimen surface thoroughly prior to milling!
Under no circumstances may the
miller cutting edge be positioned more
than a few microns (= selected milling
thickness) below the specimen surface when starting the milling procedure.
When spraying alcohol to moisten the
specimen surface, remove excess liquid as soon as it starts accumulating.
Do not operate near open flames or
near other sources of ignition!
No liquids may enter the interior of the
instrument during operation!
Leica SP2600 Ultramilling attachment
11
2. Safety
Safety instructions working with the instrument
Do not exceed a milling thickness setting of 5 µm with the following materials: brittle, very ductile materials such
as titanium or fiberglass and compound samples containing such materials (such as printed circuit boards).
During milling:
switch on the vacuum cleaner,
wear appropriate safety goggles,
wear appropriate mask (type of
mask depends on the material being
milled / size of the chips that form
during milling).
Goggles and mask prevent milling
chips and microdust from entering
your eyes and from being inhaled.
Caution - rotating miller:
After switching off the milling spindle
(button (37) - chapter. 5.4.11, p. 34 - instruction manual Leica SM2500) the
miller continues to rotate for a little
while.
When switching off the mains switch
on the control unit (see chapter 5.2, p.
21 - Instruction manual Leica SM2500)
the milling spindle will rotate briefly.
Therefore, never switch off the control
unit, while one of your hands is in the
rotation zone of the miller.
- Risk of injury!
Never touch the milling spindle while
it is rotating and/or while the plexiglass cover is shut, no matter whether
the spindle is rotating or not.
Safety instructions cleaning disinfection and maintenance
Only authorized service technicians
may access the internal components of
the instrument for service and repair!
Cleaning detergents appropriate for
the ultramilling attachment.
Varnished surfaces:
mild laboratory detergents (slightly
moistened cloth)!
Non-varnished metal parts:
mild laboratory detergents,
xylene substitutes (slightly
moistened cloth)
alcohol (slightly moistened cloth).
For disinfection of the instrument and
accessories use Leica Cryofect or
other commercial disinfectants. - Use
all disinfectants according to the
manufacturers instructions.
When disinfecting, wear appropriate
goggles and mask as well as protective gloves.
Dispose of potentially infectious
specimen material as per the laboratory regulations applicable in the
country of operation.
No liquids may enter the interior of the
instrument during cleaning, disinfection or maintenance!
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Supplementary instruction manual V 1.2 07/2002
3. Technical Data
Ultramilling attachment
Milling spindle (special design)......................................................................................... type TSAV 60 x 160
Motor, rotation speed selectable in 100-rpm steps ............................................................ 500 to 3.000 rpm
Protective class ................................................................................................................................................... I
Pollution degree.................................................................................................................................................. 2
Dimensions and weight - ultramilling attachment
Dimensions (H x W x D) ..................................................................................................... 300 x 315 x 240 mm
Weight ........................................................................................................................................................... 18 kg
Vacuum cleaner Fakir S20
Nominal voltage ........................................................................................................................................... 230 V
Nominal frequency ......................................................................................................................................50 Hz
Power draw ............................................................................................................................................ max. 4 A
These specifications are supplemented by the specifications contained in the Leica SM2500 instruction manual.
Leica SP 2600 Ultramilling attachment
13
4. Installation
4.1 Overview - Leica SP2600 Ultramilling attachment / basic instrument Leica SM2500
- instrument parts / functions
1.4
1.2
1.5
Fig. 4.1 1
1.1
2
2.1 2.2
1.3
1. Leica SM2500 - basic instrument (sliding microtome)
1.1 Leica SP2600 - ultramilling attachment
1.2 Carrier for ultramilling attachment SP2600 (when not in use)
1.3 Specimen holder system (not part of standard delivery)
1.4 Carrying handle, black, rear (extendible - not visible in Fig. 4.1)
1.5 Carrying handle, black, front (extendible)
2. Leica SM2500 - control unit
2.1 Control panel
2.2 Emergency stop button
3. Foot switch (not shown in Fig. 4.1 - for details on the foot switch please
refer to p. 18, chapter 4.6 of this supplementary instruction manual and to
p. 18, chapter 4.5.2 and p. 24, chapter 5.4.1 of the Leica SM2500 manual).
14
Supplementary instruction manual V 1.2 07/2002
4. Installation
4.2 Leica SP2600 standard delivery
Standard delivery includes:
1 Basic instrument Leica SP2600 (Ultramilling
attachment)
1 Calibrating device with dial gauge for miller
adjustment
3 Holders, each with two springs, for pre- and
finishing millers, one holder delivered attached
to the instrument.
1 Carrier for Leica SP2600 Ultramilling device
when not in use
1 Tool set:
1 Allen key, size 2.5, with handle
1 Allen key, size 4.0
1 Allen key, size 4.0 with handle
1 Allen key, size 5.0 with handle
1 Allen key, size, 8.0
1 Power cord type KN 2/5 7,5 V
1 Box level
1 Plexiglass block, 19 x 33 x 53 mm
1 Balance weight, installed in one of the miller
holders
1 Flat belt
1 Dust cover
1 Supplementary instruction manual for Leica
SP2600, to be used in conjunction with the
Leica SM2500 instruction manual
- German
- English
Millers and specimen holders are not
included in the instrument standard
delivery but have to be ordered separately, in accordance with each users
specific application(s).
Mounting instructions for all accessories and/or references to the corresponding chapters of the instruction
manual Leica SM2500 can be found in
chapter 5.6 of this instruction manual.
4.3 Site requirements
For site requirements, please refer to chapter 4.2
of the Leica SM2500 instruction manual.
4.4 Unpacking, transport, installation
Be sure to follow the unpacking instructions (attached to the outside of
the instrument shipping crate)!
Never remove or modify any of the
safety devices installed on the instrument and/or accessories!
The Leica SP2600 ultramilling attachment is supplied only in conjunction
with the Leica SM2500 microtome
(Leica SM2500 microtomes already installed at a customers site must be returned to the manufacturer for upgrading with a Leica SP2600 ultramilling
attachment).
For instructions on how to install / set up the Leica SM2500 please refer to chapter 4 of the Leica
SM2500 instruction manual.
Leica SP2600 Ultramilling attachment
15
4. Installation
4.5 Installing the Leica SP2600 ultramilling attachment
Place the carrier (1, Fig. 4.2) for the ultramilling attachment (3, Fig. 4.3) on
the rear part of the Leica SM2500 microtome fitting the two pins of the
carrier into the corresponding bores in the Leica SM2500 microtome.
1 2
Fig. 4.2
Lower the bearing blocks (2) of the microtome as far as they will go (use
button (51), chapter 5.4.13, p. 36 - Leica SM2500 manual).
Prior to placing the ultramilling attachment onto the microtome:
Make sure the bearing blocks of the microtome supporting the
milling attachment and the bearing surfaces of the milling attach-
34
ment are clean.
Two people are required to place the milling attachment on the
bearing blocks from above.
Do not slide the milling attachment onto the bearing blocks to prevent the bearing surfaces from getting scratched!
The bearing surfaces must not become damaged under any circumstances!
Fig. 4.3
16
Place ultramilling device (3) onto the microtome bearing blocks (two
people!).
Lift lid (4) of the milling attachment (serves as a handle - see Fig. 4.3).
Caution:
When releasing the lid of the milling attachment (can be lifted and
5
used as a handle) be careful not to get your fingers caught between the lid and the body of the milling attachment!
Fasten the ultramilling attachment to the microtome with four screws (5)
using a size 8 Allen key.
Connect the connecting cable of the milling attachment to the control
unit (see chapter 4.5.2, page 18 of the Leica SM2500 manual).
Supplementary instruction manual V 1.2 07/2002
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