Leica SM2500 User Manual
Instruction manual
Leica SM2500
V1.3 English - 10/2007
Always keep this manual near the instrument.
Read carefully prior to operating the instrument.
Leica SM2500
Heavy-duty
sliding microtome
NOTE
The information, numerical data, notes and value
judgments contained in this manual represent the
current state of scientific knowledge and stateof-the-art technology as we understand it following thorough investigation in this field.
We are under no obligation to update the present
manual according to the latest technical developments, nor to provide our customers with additional copies, updates etc. of this manual.
For erroneous statements, drawings, technical
illustrations etc. contained in this manual we exclude liability as far as permissible according to
the national legal system applicable in each individual case. In particular, no liability whatsoever
is accepted for any financial loss or consequential damage caused by or related to compliance
with statements or other information in this manual.
Statements, drawings, illustrations and other information as regards contents or technical details of the present manual are not to be considered as warranted characteristics of our
products.
These are determined only by the contract provisions agreed between ourselves and our customers.
Leica reserves the right to change technical
specifications as well as manufacturing processes without prior notice. Only in this way is it possible to continuously improve the technology and
manufacturing techniques used in our products.
This document is protected under copyright laws.
Any copyrights of this document are retained by
Leica Biosystems Nussloch GmbH.
Any reproduction of text and illustrations (or of
any parts thereof) by means of print, photocopy,
microfiche, web cam or other methods – including any electronic systems and media – requires
express prior permission in writing by Leica Biosystems Nussloch GmbH.
For the instrument serial number and year of
manufacture, please refer to the name plate at
the back of the instrument.
© Leica Biosystems Nussloch GmbH
Published by:
Leica Biosystems Nussloch GmbH
Heidelberger Str. 17 - 19
D-69226 Nussloch
Germany
Telephone: +49 (0)6224 143-0
Facsimile: +49 (0)6224 143-200
Internet: http://www.histo-solutions.com
Leica SM2500
3
Table of contents
1. Important information ................................................................................................................ 6
1.1 Symbols used in the text and their meanings ............................................................................................ 6
1.2 Designated use ............................................................................................................................................... 6
1.3 Information on this instruction manual ....................................................................................................... 7
2. Safety ............................................................................................................................................ 8
2.1 Safety regulations .......................................................................................................................................... 8
2.2 Built-in safety devices ................................................................................................................................... 9
2.3 Safety instructions ....................................................................................................................................... 10
3. Technical data ............................................................................................................................ 13
4. Setup ............................................................................................................................................. 14
4.1 Overview - instrument parts / functions ................................................................................................... 14
4.2 Site requirements ......................................................................................................................................... 14
4.3 Standard delivery Leica SM2500 ............................................................................................................... 15
4.4 Unpacking, transport, installation .............................................................................................................. 16
4.5 Electrical connection ................................................................................................................................... 17
4.5.1 Verifying and adjusting the voltage selector ........................................................................................... 17
4.5.2 Installing power cord, accessory cords and connecting cable to the microtome ............................ 18
4.5.3 Connecting a remote alarm device or remote camera shutter............................................................. 19
5. Operation ................................................................................................................................... 20
5.1 Overview of chapter 5 ‘Operation’ ............................................................................................................. 20
5.2 Switching the instrument on ....................................................................................................................... 21
5.3 Overview control panel - by functional areas.......................................................................................... 22
5.4 Description of each individual control element....................................................................................... 24
5.4.1 Foot switch .................................................................................................................................................... 24
5.4.2 Area 1 - Standby on/off; emergency stop ................................................................................................. 25
5.4.3 Area 2.1 - Modes of operation .................................................................................................................... 26
5.4.4 Rotary-type selector switch in area 2 ....................................................................................................... 27
5.4.5 Area 2.2 - Section/milling thickness, section/milling stroke preselection, retraction ....................... 28
5.4.6 Area 2.3 - Sectioning / milling modes ........................................................................................................ 29
5.4.7 Area 2.4 - Sledge speed .............................................................................................................................. 30
5.4.8 Area 2.5 - Setting the stop or photo position............................................................................................ 31
5.4.9 Area 2.5 - Changing the dwelling and triggering time in photo position.............................................. 32
5.4.10 Area 2.6 - Start / stop sectioning / milling................................................................................................. 33
5.4.11 Area 3 - Operating optional supplementary instruments ....................................................................... 34
5.4.12 Area 4 - Section/milling stroke counter and thickness totalizer and reset buttons .......................... 35
5.4.13 Area 5.1 - Manual movement of knife / miller .......................................................................................... 36
5.4.14 Area 5.2 - Manual movement of the sledge ............................................................................................. 37
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Instruction manual V 1.3 – 10/2007
Table of contents
5.5.1 Mounting the base plates ........................................................................................................................... 38
5.5.2 Clamping specimens or specimen stages in the base plate with vise clamp .................................... 40
5.5.3 Mountable jaws for vise .............................................................................................................................. 42
5.5.4 Mounting specimen holders onto the base plate with dovetail guide ................................................ 42
5.5.5 Specimen orientation................................................................................................................................... 45
5.5.6 Mounting the knife holders ......................................................................................................................... 46
5.5.7 Inserting knives into the holders ................................................................................................................ 46
5.5.8 Mounting knife holder A .............................................................................................................................. 47
5.5.9 Mounting knife holder B .............................................................................................................................. 50
5.5.10 Mounting knife holder C .............................................................................................................................. 52
5.5.11 Fixed-setting declination blocks (45°) and knife holder A or C ............................................................. 55
5.5.12 Working with fixed-setting (45°) declination blocks and knife holder B ............................................. 58
5.5.13 Inserting the disposable blade rail (only in knife holder B) ................................................................... 59
5.5.14 Working with distance blocks for sectioning very high specimen blocks.......................................... 59
5.6 Working with the instrument ...................................................................................................................... 60
5.6.1 Switching the instrument on / selecting parameters ............................................................................. 60
5.6.2 Trimming ........................................................................................................................................................ 61
5.6.3 Sectioning ...................................................................................................................................................... 62
5.7 Switching the instrument off ...................................................................................................................... 63
6. Trouble shooting ...................................................................................................................... 64
6.1 Error codes .................................................................................................................................................... 64
6.2 Instrument / accessories not working ...................................................................................................... 64
6.3 Power failure ................................................................................................................................................. 64
7. Cleaning, disinfection and maintenance ............................................................................ 65
7.1 Cleaning / disinfection ................................................................................................................................. 65
7.2 Maintenance ................................................................................................................................................. 67
7.2.1 General maintenance steps ........................................................................................................................ 67
7.2.2 Replacing the fuses...................................................................................................................................... 68
7.2.3 Lubricating the bearing points and the nut of the micrometer spindle ............................................... 70
8. Overview - accessories .......................................................................................................... 71
8.1 Knife holders, specimen holders, additional accessories ..................................................................... 71
8.2 Overview - which knife to be used in which knife holder ......................................................................73
9. Warranty and service .............................................................................................................. 74
10. EC Declaration of Conformity.................................................................................................75
11. Peoples Republic of China ..................................................................................................... 76
Leica SM2500
5
1. Important information
1.1 Symbols used in the text and their meanings
Warnings and cautions appear in a
grey box and are marked by a warning
triangle.
Failure to comply with these warnings/
cautions can lead to accidents, bodily
injury and damage to instrument and/
or accessories.
Notes, i.e. important information for the
user, appear in a grey box and are
marked by an information sign.
Figures in brackets refer to item num-
(5)
bers in illustrations or to the illustrations themselves.
1.2 Designated use
Fig. 1
The Leica SM 2500 is a microprocessor-controlled, electrically driven heavy-duty sliding microtome, designated to produce thin sections of
industrial materials and paraffin-, celloidin- and
plastic-embedded samples in medicine, biology
and industry.
In connection with the Leica SP2600 ultramilling
device, the unit is used for surface milling of plastic, metal and organic-material specimens (such
as bones, teeth). - The Leica SP2600 is described
in a separate instruction manual.
ENTER
6
Function keys that need to be pressed
on the control unit are printed in bold
and capital letters.
The Leica SM 2500 may be used only in conjunction with accessories and supplementary instruments made by Leica.
If the instrument is used for any other than
the designated application, this will be
considered an improper use of the product!
Instruction manual V 1.3 – 10/2007
1.3 Information on this instruction manual
Instrument type
All information provided in this instruction manual applies only to the instrument type indicated on the title page.
Name plates indicating the instrument type and serial number are attached
to the right side of the microtome and to the left side of the control unit.
1. Important information
Microtome Control unit
Fig. 2
Fig. 3
Working with this instruction manual
This manual contains important instructions and information related to the
operating safety and maintenance of the instrument.
It is an important part of the product, which must be read carefully prior to
setting up and operating the instrument and it should be kept near the instrument at all times.
If additional requirements on accident prevention and environmental protection exist in the country of operation, this instruction
manual must be supplemented by appropriate instructions to ensure compliance with such requirements.
Leica SM2500
7
2. Safety
2.1 Safety regulations
This instrument has been built in accordance with the German version of the
following directives and standards:
1. Council Directive 98/37/EC - Machinery
• DIN EN 292-1 - Part 1
• DIN EN 292-2 and A1 - Part 2
• DIN EN 294
• DIN EN 349
• DIN EN 418
• DIN EN 1037
• DIN EN 1050
2. Council Directive 73/23/EEC - Low voltage
• DIN EN 61010-1
• DIN EN 61010-1/A2 - Part 1
3. Council Directive 89/336/EEC - Electromagnetic compatibility
• DIN EN 61326 - Amendment A1
• DIN EN 61326/A1 - Amendment A1
• DIN EN 61000-3-2 - Part 3 - Main section 2
• DIN EN 6100-3-2 - Part 3-2
• DIN EN 6100-3-3 - Part 3-3 - Main section 3
In order to maintain this condition and to ensure safe operation, the operator
must observe the instructions and warnings contained in this instruction
manual.
8
Instruction manual V 1.3 – 10/2007
2.2 Built-in safety devices
The instrument is equipped with the following safety features and devices:
Emergency stop button located on the control panel:
• For a detailed functional description of the emergency stop switch
please refer to chapter 5.4.2, page 23.
Emergency stop function of the foot switch:
• For a detailed functional description of the foot switch and the emergency stop function integrated into the foot switch, please refer to chapter 5.4.1, page 22.
Integrated knife guards of the different knife holders
• Detailed descriptions of the knife guards integrated into each knife
holder are provided in chapter 5.5.
The safety devices provided by the manufacturer constitute only a basis for
accident prevention.
Major responsibility for accident prevention during the use of the instrument
rests with the employer who has the operating authority for the instrument
and, in addition, with the persons, designated by him, who operate, service
and repair the instrument.
To ensure safe and trouble-free operation of the instrument, be sure to
comply with all instructions, warning and cautions in the following chapters!
2. Safety
Leica SM2500
9
2. Safety
2.3 Safety instructions
Be sure to read and comply with the
safety instructions, warnings and cautions in this chapter, even if you are already familiar with the operation and
use of other Leica products.
Safety instructions:
Transport and installation
Be sure to follow the unpacking instructions (attached to the outside of
the instrument shipping crate)!
When transporting the instrument, always lock the extendible handles in
place by attaching the transport safety
devices (white plastic blocks) - for details on how to secure the extendible
handles, please refer to chapter 4.3
‘Unpacking, transport, installation’.
The instrument may only be transported in an upright position!
Two people are required to lift and/or
carry the instrument (instrument
weight!)
Be sure to comply with the site requirements (chapter 4.2)!
Never remove or modify any of the
safety devices installed on the instrument and/or accessories!
Verify the voltage selector setting:
The voltage selector comes factoryadjusted to a setting of 230 V.
Prior to connecting the instrument to
mains, verify whether the voltage setting is correct for the mains electricity
in your laboratory!
Incorrect voltage selector settings can
lead to severe damage to the instrument!
Prior to adjusting the voltage selector,
disconnect the instrument from mains!
For details on verification and adjustment of the voltage selector, please refer to chapter 4.4.1 ‘Verifying and adjusting the voltage selector’.
The instrument may be connected to
mains only with one of the power
cords supplied and only to grounded
sockets!
The instrument may not be operated in
hazardous locations where risk of explosion exists!
Extreme temperature fluctuations and
high air humidity (e.g. transport, storage) may cause condensation inside
the instrument.
In this case, wait at least two hours
before switching the instrument on.
Failure to comply with this waiting period may cause severe damage to the
instrument!
10
Instruction manual V 1.3 – 10/2007
Safety instructions:
Operating the instrument
The instrument may only be operated
by skilled laboratory personnel. It may
only be used for the designated application and operated in accordance
with the instructions contained in this
manual!
Before mounting the knife holder make
sure the bearing blocks of the microtome supporting the knife holder are
clean.
Carefully place the preassembled
knife holder onto the microtome bearing blocks from above. Do not slide the
knife holder onto the bearings to prevent the bearing surface from getting
scratched.
Handle microtome knives and blades
with utmost care. Knife and blade
edges are extremely sharp and can
cause severe injuries!
2. Safety
Never leave knives/disposable blades
or knife holders with a knife/disposable blade inserted lying around!
Always store knives in the knife case
when not in use!
Never place a knife on a bench with
the edge facing upwards!
Never try to catch a falling knife or
disposable blade!
Always clamp the specimen first and
the knife second!
Always wear protective glasses when
sectioning brittle specimens! - Risk of
splintering!
No liquids may enter the interior of the
instrument during operation!
Always cover the knife edge with the
knife guard during work breaks!
Wear cut-resistant safety gloves when
inserting/removing the knife (see also
chapter 8.1 ‘Additional accessories’).
Prior to inserting the knife, always
tighten one of the knife clamping
screws of the knife holder to secure
the knife holder in position!
Make sure not to misalign the knife
when tightening the knife clamping
screws as this will negatively affect
sectioning results!
Leica SM2500
Always remove the knife from the
knife holder when finishing work!
When working with the eccentric ring,
NEVER try to orient the specimen - this
will cause severe damage to the
specimen orientation mechanism!
When knife holder A or C is combined
with the declination blocks, the knife
guard cannot be placed over the knife
edge. Therefore, use extreme caution
when working with this configuration
- wear cut-resistant gloves!
11
2. Safety
Safety instructions:
Cleaning, disinfection and maintenance
Only authorized service technicians
may access the internal components of
the instrument for service and repair!
Prior to any cleaning, disinfection or
maintenance work, the instrument
must be switched off and disconnected from mains!
Exception:
Cleaning or disinfection procedures
which require the sledge to be moved.
Remove both knife and specimen prior
to any cleaning, disinfection or maintenance work!
Clean the control panel only in
standby mode or when the instrument
is switched off.
Appropriate cleaners for non-varnished metal parts (knife holders,
base plates, specimen clamps):
xylene substitutes, alcohol - apply
with an only slightly moistened cleaning cloth!
Use cleaners and disinfectants according to the safety instructions of
the manufacturer and in compliance
with the regulations applicable in
your laboratory!
Wear cut-resistant safety gloves to remove the knife (see chapter 8.1 ‘Additional accessories’).
No liquids may enter the interior of the
instrument during cleaning, disinfection or maintenance!
Clean only with a slightly moistened
cleaning cloth!
Disinfection only with disinfectants
that allow application with a slightly
moistened cloth!
Do not use any solvents (e.g. xylene,
acetone), any cleaners containing
solvents or scouring powders for
cleaning!
Appropriate cleaners for the control
unit / control panel and all painted surfaces: mild laboratory detergent
(slightly moistened cloth)!
Regularly check and empty the section waste trays located below the extendible carrying handles.
Prior to exchanging any fuse, always
switch the instrument off and unplug
the mains plug.
Replace defective fuses only with
fuses of identical specification!
Be sure to follow the instructions in
chapter 7.2.2 (selecting the correct replacement fuse for each fuse holder).
After replacing the line fuses doublecheck whether the correct voltage selector setting is visible.
Users may carry out only the maintenance work described in chapter 7.2!
Any other maintenance work must be
carried out by authorized Leica service technicians.
12
Instruction manual V 1.3 – 10/2007
3. Technical data
Microtome
Section thickness range ...........................................................0 μm to 1.000 μm, adjustable in 1-μm steps
Total horizontal specimen stroke .......................................................................................maximum 275 mm
Total vertical knife feed ........................................................................................................................... 70 mm
Knife retraction (during specimen return stroke) ...................................................................... 0 - 1,000 μm
Clearance angle adjustment ............................................................................................................. 0 ° to 17 °
Knife declination - fixed setting (declination blocks = optional accessory) ....................................... 45 °
Maximum specimen size (L x W x H) ........................................................................................ 25 x 20 x 7 cm
Specimen orientation (along x / y axis) ..................................................................... 4,8 ° along each axis
Specimen orientation (rotability) ................................................................................. approx. +/- 3 and 90 °
Sectioning speed ................................................................... 0.5 - 100 mm/s, adjustable in 0.1-mm steps
Return speed ................................................................... 0.5 - 100 mm/s, adjustable in 0.1-mm steps
Manual knife movement (slow / fast) ......................................................................... 37 mm/s and 74 mm/s
Manual specimen movement (slow / fast)................................................................. 37 mm/s and 74 mm/s
Electrical connections
Nominal voltage .............................................................................................................. 100 / 120 / 230 / 240 V
Nominal frequency ...................................................................................................................50 Hz and 60 Hz
Maximum power draw ......................................................................................................................... 1,400 VA
Main fuses, type MDA, Fa. Bussmann .............................................................................................. 2 x T10A
Protective class .................................................................................................................................................. I
Overvoltage installation category ...................................................................................................................II
Vacuum cleaner ........................................................................ 100 / 120 V - maximum power draw 500 VA
Vacuum cleaner ..................................................................... 230 / 240 V - maximum power draw 1,200 VA
Lamp .......................................................................................... 100 / 120 V - maximum power draw 100 W
Lamp .......................................................................................... 230 / 240 V - maximum power draw 200 W
Dimensions and weights
Microtome (H x W x L) ....................................................................................................... 250 x 390 x 750 mm
Control unit (H x W x D) ...................................................................................................... 220 x 385 x 510 mm
Required bench top space for microtome and control unit ................................................ 1,000 x 950 mm
Microtome ..................................................................................................................................... approx. 75 kg
Control unit .................................................................................................................................... approx. 23 kg
Leica SM2500
13
4. Setup
4.1 Overview - instrument parts / functions
1.3 1.11.2 1 2.1 2.22
Fig. 3a
1.4
1. Leica SM2500 - Microtome
1.1 Knife holder system (not part of standard delivery)
1.2 Specimen holder system (not part of standard delivery)
1.3 Carrying handle, rear (extendible - not visible in Fig. 3a)
1.4 Carrying handle, front (extendible)
2. Leica SM2500 - control unit
2.1 Control unit
2.2 Emergency stop button
3. Foot switch (not visible in Fig. 3a see chapter 5.4.1 for details)
4.2 Site requirements
• Bench top surface for microtome and control unit sized approx. 1,000 x 950 mm
• Stable laboratory bench (total weight of microtome and control unit approx. 100 kg), exactly horizontal or horizontally adjustable.
• At least 15 cm space on all sides of both instruments to ensure that air can circulate freely around
the instruments.
• Virtually vibration-free floor.
• Stable ambient temperature between +18 °C and +35 °C.
• Relative air humidity of maximum 60 %, non-condensing.
• Do not place near sources of vibration, avoid exposure to direct sunlight and marked temperature
fluctuations.
14
Instruction manual V 1.3 – 10/2007
4.2 Standard delivery Leica SM2500
1 Basic instrument
1 Separate control unit ............................................................................. 0405 42863
1 Connecting cable for control unit ........................................................ 0405 35685
1 Foot switch (0505 33888)
1 Set of power cords:
-1 Power cord “D” ............................................................................... 0411 13558
-1 Power cord “USA-C-J” .................................................................. 0411 13559
-1 Power cord “UK” ST/BU F-5A....................................................... 0411 27822
1 Set of adapter cables:
-2 Adapter cables “USA” ................................................................... 0411 36234
-2 Adpater cables “EU” ...................................................................... 0411 36235
1 Set of replacement fuses:
-2 x T 1.0 A ............................................................................................ 6943 01001
-2 x T 6.25A ........................................................................................... 6943 06251
-2 x T 10.0 A .......................................................................................... 6943 10001
1 Tool set:
-1 Allen key, size 2.5 ............................................................................ 0194 13195
-1 Allen key, size 3.0 ............................................................................ 0194 04764
-1 Allen key, size 4.0 ............................................................................ 0194 04782
-1 Allen key, size 5.0 ............................................................................ 0194 04760
-1 Allen key, size 8.0 ............................................................................ 0194 04792
-1 Torque wrench, size 32 .................................................................. 0383 11934
-1 Single-head wrench, size 13 ......................................................... 0330 11936
-1 Screw driver .................................................................................... 0170 12988
-1 Clamping device .............................................................................. 0383 32392
-1 Remote alarm jack 6.3 mm............................................................. 6844 01005
-1 Jack connector 3-contact .............................................................6844 01008
1 Brush ........................................................................................................ 0183 04477
1 Grease gun .............................................................................................. 0383 11896
1 Dust cover for microtome ..................................................................... 0212 11725
1 Dust cover for control unit .................................................................... 0212 36428
1 Rubber mat .............................................................................................. 0405 36405
1 Instruction manual for Leica SM2500 ................................................. 0701 35100
4. Setup
Specimen and knife holder systems are not included in the instrument standard delivery. They
have to be ordered separately, in accordance with each user’s specific application(s).
Mounting instructions for all accessories can be found in chapter 5.5 of this instruction
manual.
Leica SM2500
15
4. Setup
4.4 Unpacking, transport, installation
2
1
2
1
Fig. 4
3
4
• Pull out the carrying handles (1) at the front and rear of the instrument.
• Remove the upper housing parts (2) at the front and rear.
• Secure the carrying handles (1) placing the white plastic blocks (3) on
Be sure to follow the unpacking instructions (attached to the outside of the instrument shipping crate)!
When transporting the instrument, always lock the extendible
carrying handles in place by attaching the transport safety devices (white plastic blocks)!
the telescopic bars (4).
Two persons are needed to lift and/or carry the instrument (instrument weight!).
The instrument may only be transported in an upright position!
• Once the instrument has been placed onto the bench, remove the two
plastic blocks (3) from the telescopic bars (4) and put them back into
their storage place in the instrument housing.
• Put the upper housing parts (2) back in place and push the carrying
handles (1) inwards as far they will go.
Fig. 5
34
16
Prior to connecting the instrument to mains, verify whether the
voltage setting is correct for the mains electricity in your lab
(please refer to chapter 4.4.1 ‘Verifying and adjusting the voltage
selector’)!
The instrument may not be operated in hazardous locations where
risk of explosion exists!
Never remove or modify any of the safety devices installed on the
instrument and/or accessories!
Fig. 6
Instruction manual V 1.3 – 10/2007
4.5 Electrical connection
4.5.1 Verifying and adjusting the voltage selector
The voltage selector comes factory-adjusted to a setting of 230 V.
Prior to connecting the instrument to mains, verify whether the
voltage setting is correct for the mains electricity in your lab!
Incorrect voltage selector settings can lead to severe damage to
the instrument!
Prior to adjusting the voltage selector, disconnect the instrument
from mains!
Checking the voltage selector setting:
The voltage selector is located above the mains switch, on the rear left of
the control unit.
• Does the selected setting (1) correspond to the nominal voltage in your
laboratory?
1
• If yes: --> proceed to chapter 4.4.2 on the following page.
• If no: --> the voltage selector must be changed to the correct setting as
follows:
• Insert a small screw driver into the notch at the lower end of the voltage
selector housing and carefully remove the housing, using the screwdriver as a lever.
• Remove housing (2) together with fuses (3) and the voltage selector
342
1
Fig.7
block (4) (white color).
• Remove the fuses.
• Remove the voltage selector block and re-insert it so that the correct
setting, corresponding to the mains voltage in your lab, is visible in window (1). (Selectable voltage settings: 100, 120, 230 and 240 V).
• Re-insert the fuses.
• Re-insert the housing together with the voltage selector block and the
fuses into the instrument and push slightly inwards until the voltage selector housing locks in place (audible click).
• Verify once again, whether the setting that shows in window (1) is appropriate for the mains voltage in your lab.
4. Setup
Leica SM2500
17
4. Setup
4.5.2 Installing power cord, accessory cords and connecting cable to the microtome
At the rear of the control unit, set up the following connections:
• Remove the tape from the mains inlet for the power cord (8).
• From the set of power cords delivered (the instrument is delivered with
several, country-specific power cords), select the one that is appropriate
for your lab; connect the power cord.
Fig. 8
Do not yet plug the power cord into the mains outlet.
Connect the remaining cables:
Fig.
9
• Install the connecting cable (1) leading to the microtome and tighten the
132
5
674
8
fixing screws.
• Connect the other end of cable (1) to the right side of the microtome and
tighten the fixing screws.
• The connecting cable to the ultramiller (2) needs to be installed only
when working with the Leica SP2600 --> see separate instruction manual
Leica SP2600.
• Connect photo camera (3) (optional).
• Connect remote alarm (4) (optional).
• Connect foot switch (5) (NOT optional).
18
The foot switch must be connected - otherwise the instrument will
not be operational.
• Connect the vacuum cleaner (6) (optional with Leica SM2500 - necessary
when working with Leica SP2600).
• Connect the lamp (7) (optional).
Instruction manual V 1.3 – 10/2007
4.5.3 Connecting a remote alarm device or remote camera shutter
Connecting a remote alarm device:
A remote alarm device can be connected to the Leica SM2500 via a 6.3 mmdiameter jack connector (optional accessory).
• For details on how to connect a remote alarm device to the Leica
SM2500, please contact your local Leica office or the manufacturer
Leica Biosystems Nussloch directly.
Connecting a camera shutter:
The Leica SM2500 offers the option to automatically trigger a photocamera
shutter each time the specimen sledge reaches a preselected photo position.
The connecting cable leading from the camera to the Leica SM2500 must be
configured individually, depending on the type of camera used.
• For on this option, please contact your local Leica office or the manufac-
turer Leica Biosystems Nussloch directly.
4. Setup
Leica SM2500
19
5. Operation
5.1 Overview of chapter 5 ‘Operation’
Chapter 5 ‘Operation’ is structured as follows:
Chapter 5.1 - Structural description of chapter 5.
Chapter 5.2 - Switching the instrument on.
Chapter 5.3 - Overview - control panel.
Chapter 5.4 - Detailed description of each individual control element
Chapter 5.5 - Mounting the accessories (specimen holders,
Chapter 5.6 - Working with the instrument:
Chapter 5.7 - Switching the instrument off
Initial operation:
The first time you operate the instrument we recommend you work through
chapters 5.1, 5.2, 5.4 and 5.5, in that order.
Pay particular attention to the safety instructions.
Once you are familiar with the control panel functions and know how to in-
stall the accessories, proceed to chapters 5.6 / 5.7.
(controls numbered from 1 to 62;
control panel divided into functional areas 1 to 5).
knife holders, knives).
short description of a typical application, including
references to detailed descriptions of individual steps
given in other chapters.
20
Daily routine work:
The control panel overview (chapter 5.3 on pages 20/21) will be a useful tool
for your daily routine work.
Instruction manual V 1.3 – 10/2007
5.2 Switching the instrument on
• Switch on the mains switch (0/I) located at the rear side of the control
panel (On = switch position ‘I’):
All control elements on the control unit will emit light for about 2 secs.
Next, the software version is displayed in the section counter (no. 44,
upper display in functional area 4 - chapter 5.4.12).
5. Operation
Caution:
Did you verify whether the voltage selector is set correctly? If not, please refer to chapter 4.4.1!
Incorrect voltage selector settings can lead to severe damage to
the instrument!
The instrument may be connected to mains only with one of the
power cords supplied and only to grounded sockets!
The instrument may not be operated in hazardous locations where
risk of explosion exists!
Extreme temperature fluctuations and high air humidity (transport,
storage) may cause condensation inside the instrument.
In this case, wait at least two hours before switching the instrument on.
Failure to comply with this waiting period may cause severe damage to the instrument!
Leica SM2500
Fig. 10
After switching on the mains switch, the instrument will go to standby mode
(mains voltage is supplied to the control unit, the control panel is, however,
deactivated).
• To activate the control panel, press and hold the STANDBY button for
approx. 3 secs:
the control elements are activated.
Control parameters stored prior to switching the instrument off
will still be available when the instrument is switched on again.
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5. Operation
5.3 Overview control panel - by functional areas
Area 3 - includes the controls for the supplementary instruments
(41) Rotational speed of miller (RPM)
and accessories that can be attached to the Leica SM 2500,
(ultramilling attachment, vacuum cleaner, lamp).
(33/34) Lamp On/Off
Fig. 11
(40, 39) to set rotational
speed of miller
Area 4 contains two displays:
-Section counter (total number of
sections / milling strokes)
-Section thickness totalizer (total
thickness of sections / milling strokes)
Contains two reset buttons,
one for each display.
Area 5.1 contains all display elements and
control buttons for manual
movement of knife / miller
(operational in manual mode of
operation ‘MAN’).
(48) - Knife/miller fast upwards
(49) - Knife/miller slow upwards
(50) - Knife/miller slow downwards
(51) - Knife/miller fast downwards
(47) - LED
(52) - LED
(40) (39)
(56)
(57)
(58)
(59)
(60) - LED
(35/36) Vacuum cleaner
On/Off
(37/38) Miller rotation On/Off
(44) Section / milling stroke
counter
(42) Reset for counter (44)
(43) Reset for counter (45)
(45/46)Section thickness /
milling stroke
thickness totalizer
(53) - LED
(54) - LED
(55) - LED
(61/62) - Sectioning / milling
stroke limit
(56 - 59) - Manual sledge
movement
(forward/backward
/ fast / slow)
Area 5.2 contains the display and
control elements for
manual movement of the
specimen (in manual mode
of operation ‘MAN’) and
for limiting the sectioning
/ milling stroke length
(‘Sectioning window’).
Fig. 12
22
Instruction manual V 1.3 – 10/2007
5. Operation
Area 1 - to switch from standby mode to active mode (control panel activated) and back.
Emergency stop button, stops all instrument functions immediately when pressed.
Area 2.1to select a mode of
operation via button (5):
manual mode ‘MAN’ (6)
automatic mode ‘AUTO’ (7)
and
program mode ‘PROG’ (8)
Area 2.3to select a sectioning /
milling mode
via button (15):
continuous mode (16)
single mode (17) or
step-by-step mode (18)
Area 2.5 to select a stop position
(24/25) or photo position
(27/28), where the sledge
will stop.
(23) Selector button
‘Stop position’
(26) Selector button
‘Photo position’
Area 2.6 contains the buttons to start
and stop sectioning / milling
and the LEDs that indicate
sectioning / milling status.
(5)
(6)
(7)
(8)
(15)
(16)
(17)
(18)
(24)
(25)
(27)
(28)
(1) LED
(2) Standby On/Off (3) LED (4) Emergency stop button
Area 2.2selection (10) and
indication (14) of
section/milling
(11)
(12) (13) (10)
(14)
(22) (19)
(20)
(21)
thickness (11),
preselected number of
sections / milling
strokes (12) (in mode
of operation PROG
only) and knife
retraction (13).
Area 2.4to select (19) and
indicate (22)
sectioning / milling
speed (20)
and return stroke speed
(21) of the sledge
(9)
Rotary-type selector
switch (9) for
entering parameters
(11), (12) and( 13) in
area 2.2 as well as
for entering
parameters (20) and
(21) in area 2.4.
Leica SM2500
(29) (30) (32) (31)
To start: press (29) and (30) simultaneously
To stop: press either (29) or (30)
Fig. 13
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