Leica SM2500 User Manual

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Instruction manual
Leica SM2500 V1.3 English - 10/2007 Always keep this manual near the instrument. Read carefully prior to operating the instrument.
Leica SM2500
Heavy-duty
sliding microtome
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NOTE
The information, numerical data, notes and value judgments contained in this manual represent the current state of scientific knowledge and state­of-the-art technology as we understand it follow­ing thorough investigation in this field. We are under no obligation to update the present manual according to the latest technical devel­opments, nor to provide our customers with addi­tional copies, updates etc. of this manual. For erroneous statements, drawings, technical illustrations etc. contained in this manual we ex­clude liability as far as permissible according to the national legal system applicable in each indi­vidual case. In particular, no liability whatsoever is accepted for any financial loss or consequen­tial damage caused by or related to compliance with statements or other information in this man­ual. Statements, drawings, illustrations and other in­formation as regards contents or technical de­tails of the present manual are not to be consid­ered as warranted characteristics of our products.
These are determined only by the contract provi­sions agreed between ourselves and our cus­tomers. Leica reserves the right to change technical specifications as well as manufacturing process­es without prior notice. Only in this way is it pos­sible to continuously improve the technology and manufacturing techniques used in our products. This document is protected under copyright laws. Any copyrights of this document are retained by Leica Biosystems Nussloch GmbH. Any reproduction of text and illustrations (or of any parts thereof) by means of print, photocopy, microfiche, web cam or other methods – includ­ing any electronic systems and media – requires express prior permission in writing by Leica Bio­systems Nussloch GmbH. For the instrument serial number and year of manufacture, please refer to the name plate at the back of the instrument.
© Leica Biosystems Nussloch GmbH
Published by: Leica Biosystems Nussloch GmbH
Heidelberger Str. 17 - 19 D-69226 Nussloch Germany Telephone: +49 (0)6224 143-0 Facsimile: +49 (0)6224 143-200 Internet: http://www.histo-solutions.com
Leica SM2500
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Table of contents
1. Important information ................................................................................................................ 6
1.1 Symbols used in the text and their meanings ............................................................................................ 6
1.2 Designated use ............................................................................................................................................... 6
1.3 Information on this instruction manual ....................................................................................................... 7
2. Safety ............................................................................................................................................ 8
2.1 Safety regulations .......................................................................................................................................... 8
2.2 Built-in safety devices ................................................................................................................................... 9
2.3 Safety instructions ....................................................................................................................................... 10
3. Technical data ............................................................................................................................ 13
4. Setup ............................................................................................................................................. 14
4.1 Overview - instrument parts / functions ................................................................................................... 14
4.2 Site requirements ......................................................................................................................................... 14
4.3 Standard delivery Leica SM2500 ............................................................................................................... 15
4.4 Unpacking, transport, installation .............................................................................................................. 16
4.5 Electrical connection ................................................................................................................................... 17
4.5.1 Verifying and adjusting the voltage selector ........................................................................................... 17
4.5.2 Installing power cord, accessory cords and connecting cable to the microtome ............................ 18
4.5.3 Connecting a remote alarm device or remote camera shutter............................................................. 19
5. Operation ................................................................................................................................... 20
5.1 Overview of chapter 5 ‘Operation’ ............................................................................................................. 20
5.2 Switching the instrument on ....................................................................................................................... 21
5.3 Overview control panel - by functional areas.......................................................................................... 22
5.4 Description of each individual control element....................................................................................... 24
5.4.1 Foot switch .................................................................................................................................................... 24
5.4.2 Area 1 - Standby on/off; emergency stop ................................................................................................. 25
5.4.3 Area 2.1 - Modes of operation .................................................................................................................... 26
5.4.4 Rotary-type selector switch in area 2 ....................................................................................................... 27
5.4.5 Area 2.2 - Section/milling thickness, section/milling stroke preselection, retraction ....................... 28
5.4.6 Area 2.3 - Sectioning / milling modes ........................................................................................................ 29
5.4.7 Area 2.4 - Sledge speed .............................................................................................................................. 30
5.4.8 Area 2.5 - Setting the stop or photo position............................................................................................ 31
5.4.9 Area 2.5 - Changing the dwelling and triggering time in photo position.............................................. 32
5.4.10 Area 2.6 - Start / stop sectioning / milling................................................................................................. 33
5.4.11 Area 3 - Operating optional supplementary instruments ....................................................................... 34
5.4.12 Area 4 - Section/milling stroke counter and thickness totalizer and reset buttons .......................... 35
5.4.13 Area 5.1 - Manual movement of knife / miller .......................................................................................... 36
5.4.14 Area 5.2 - Manual movement of the sledge ............................................................................................. 37
Instruction manual V 1.3 – 10/2007
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Table of contents
5.5.1 Mounting the base plates ........................................................................................................................... 38
5.5.2 Clamping specimens or specimen stages in the base plate with vise clamp .................................... 40
5.5.3 Mountable jaws for vise .............................................................................................................................. 42
5.5.4 Mounting specimen holders onto the base plate with dovetail guide ................................................ 42
5.5.5 Specimen orientation................................................................................................................................... 45
5.5.6 Mounting the knife holders ......................................................................................................................... 46
5.5.7 Inserting knives into the holders ................................................................................................................ 46
5.5.8 Mounting knife holder A .............................................................................................................................. 47
5.5.9 Mounting knife holder B .............................................................................................................................. 50
5.5.10 Mounting knife holder C .............................................................................................................................. 52
5.5.11 Fixed-setting declination blocks (45°) and knife holder A or C ............................................................. 55
5.5.12 Working with fixed-setting (45°) declination blocks and knife holder B ............................................. 58
5.5.13 Inserting the disposable blade rail (only in knife holder B) ................................................................... 59
5.5.14 Working with distance blocks for sectioning very high specimen blocks.......................................... 59
5.6 Working with the instrument ...................................................................................................................... 60
5.6.1 Switching the instrument on / selecting parameters ............................................................................. 60
5.6.2 Trimming ........................................................................................................................................................ 61
5.6.3 Sectioning ...................................................................................................................................................... 62
5.7 Switching the instrument off ...................................................................................................................... 63
6. Trouble shooting ...................................................................................................................... 64
6.1 Error codes .................................................................................................................................................... 64
6.2 Instrument / accessories not working ...................................................................................................... 64
6.3 Power failure ................................................................................................................................................. 64
7. Cleaning, disinfection and maintenance ............................................................................ 65
7.1 Cleaning / disinfection ................................................................................................................................. 65
7.2 Maintenance ................................................................................................................................................. 67
7.2.1 General maintenance steps ........................................................................................................................ 67
7.2.2 Replacing the fuses...................................................................................................................................... 68
7.2.3 Lubricating the bearing points and the nut of the micrometer spindle ............................................... 70
8. Overview - accessories .......................................................................................................... 71
8.1 Knife holders, specimen holders, additional accessories ..................................................................... 71
8.2 Overview - which knife to be used in which knife holder ......................................................................73
9. Warranty and service .............................................................................................................. 74
10. EC Declaration of Conformity.................................................................................................75
11. Peoples Republic of China ..................................................................................................... 76
Leica SM2500
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1. Important information

1.1 Symbols used in the text and their meanings

Warnings and cautions appear in a grey box and are marked by a warning triangle. Failure to comply with these warnings/ cautions can lead to accidents, bodily injury and damage to instrument and/ or accessories.
Notes, i.e. important information for the user, appear in a grey box and are marked by an information sign.
Figures in brackets refer to item num-
(5)
bers in illustrations or to the illustra­tions themselves.

1.2 Designated use

Fig. 1
The Leica SM 2500 is a microprocessor-con­trolled, electrically driven heavy-duty sliding mi­crotome, designated to produce thin sections of industrial materials and paraffin-, celloidin- and plastic-embedded samples in medicine, biology and industry.
In connection with the Leica SP2600 ultramilling device, the unit is used for surface milling of plas­tic, metal and organic-material specimens (such as bones, teeth). - The Leica SP2600 is described in a separate instruction manual.
ENTER
Function keys that need to be pressed on the control unit are printed in bold and capital letters.
The Leica SM 2500 may be used only in conjunc­tion with accessories and supplementary instru­ments made by Leica.
If the instrument is used for any other than
the designated application, this will be
considered an improper use of the product!
Instruction manual V 1.3 – 10/2007
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1.3 Information on this instruction manual

Instrument type
All information provided in this instruction manual applies only to the instru­ment type indicated on the title page.
Name plates indicating the instrument type and serial number are attached to the right side of the microtome and to the left side of the control unit.
1. Important information
Microtome Control unit
Fig. 2
Fig. 3
Working with this instruction manual
This manual contains important instructions and information related to the operating safety and maintenance of the instrument.
It is an important part of the product, which must be read carefully prior to setting up and operating the instrument and it should be kept near the in­strument at all times.
If additional requirements on accident prevention and environmen­tal protection exist in the country of operation, this instruction manual must be supplemented by appropriate instructions to en­sure compliance with such requirements.
Leica SM2500
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2. Safety

2.1 Safety regulations

This instrument has been built in accordance with the German version of the following directives and standards:
1. Council Directive 98/37/EC - Machinery
DIN EN 292-1 - Part 1
DIN EN 292-2 and A1 - Part 2
DIN EN 294
DIN EN 349
DIN EN 418
DIN EN 1037
DIN EN 1050
2. Council Directive 73/23/EEC - Low voltage
DIN EN 61010-1
DIN EN 61010-1/A2 - Part 1
3. Council Directive 89/336/EEC - Electromagnetic compatibility
DIN EN 61326 - Amendment A1
DIN EN 61326/A1 - Amendment A1
DIN EN 61000-3-2 - Part 3 - Main section 2
DIN EN 6100-3-2 - Part 3-2
DIN EN 6100-3-3 - Part 3-3 - Main section 3
In order to maintain this condition and to ensure safe operation, the operator must observe the instructions and warnings contained in this instruction manual.
Instruction manual V 1.3 – 10/2007
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2.2 Built-in safety devices

The instrument is equipped with the following safety features and devices:
Emergency stop button located on the control panel:
• For a detailed functional description of the emergency stop switch please refer to chapter 5.4.2, page 23.
Emergency stop function of the foot switch:
• For a detailed functional description of the foot switch and the emer­gency stop function integrated into the foot switch, please refer to chap­ter 5.4.1, page 22.
Integrated knife guards of the different knife holders
• Detailed descriptions of the knife guards integrated into each knife holder are provided in chapter 5.5.
The safety devices provided by the manufacturer constitute only a basis for accident prevention.
Major responsibility for accident prevention during the use of the instrument rests with the employer who has the operating authority for the instrument and, in addition, with the persons, designated by him, who operate, service and repair the instrument.
To ensure safe and trouble-free operation of the instrument, be sure to comply with all instructions, warning and cautions in the following chap­ters!
2. Safety
Leica SM2500
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2. Safety

2.3 Safety instructions

Be sure to read and comply with the safety instructions, warnings and cau­tions in this chapter, even if you are al­ready familiar with the operation and use of other Leica products.
Safety instructions: Transport and installation
Be sure to follow the unpacking in­structions (attached to the outside of the instrument shipping crate)!
When transporting the instrument, al­ways lock the extendible handles in place by attaching the transport safety devices (white plastic blocks) - for de­tails on how to secure the extendible handles, please refer to chapter 4.3 ‘Unpacking, transport, installation’.
The instrument may only be trans­ported in an upright position!
Two people are required to lift and/or carry the instrument (instrument weight!)
Be sure to comply with the site re­quirements (chapter 4.2)!
Never remove or modify any of the safety devices installed on the instru­ment and/or accessories!
Verify the voltage selector setting: The voltage selector comes factory­adjusted to a setting of 230 V. Prior to connecting the instrument to mains, verify whether the voltage set­ting is correct for the mains electricity in your laboratory! Incorrect voltage selector settings can lead to severe damage to the instru­ment! Prior to adjusting the voltage selector, disconnect the instrument from mains!
For details on verification and adjust­ment of the voltage selector, please re­fer to chapter 4.4.1 ‘Verifying and ad­justing the voltage selector’.
The instrument may be connected to mains only with one of the power cords supplied and only to grounded sockets!
The instrument may not be operated in hazardous locations where risk of ex­plosion exists!
Extreme temperature fluctuations and high air humidity (e.g. transport, stor­age) may cause condensation inside the instrument. In this case, wait at least two hours before switching the instrument on. Failure to comply with this waiting pe­riod may cause severe damage to the instrument!
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Instruction manual V 1.3 – 10/2007
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Safety instructions: Operating the instrument
The instrument may only be operated by skilled laboratory personnel. It may only be used for the designated appli­cation and operated in accordance with the instructions contained in this manual!
Before mounting the knife holder make sure the bearing blocks of the micro­tome supporting the knife holder are clean.
Carefully place the preassembled knife holder onto the microtome bear­ing blocks from above. Do not slide the knife holder onto the bearings to pre­vent the bearing surface from getting scratched.
Handle microtome knives and blades with utmost care. Knife and blade edges are extremely sharp and can cause severe injuries!
2. Safety
Never leave knives/disposable blades or knife holders with a knife/dispos­able blade inserted lying around!
Always store knives in the knife case when not in use!
Never place a knife on a bench with the edge facing upwards!
Never try to catch a falling knife or disposable blade!
Always clamp the specimen first and the knife second!
Always wear protective glasses when sectioning brittle specimens! - Risk of splintering!
No liquids may enter the interior of the instrument during operation!
Always cover the knife edge with the knife guard during work breaks!
Wear cut-resistant safety gloves when inserting/removing the knife (see also chapter 8.1 ‘Additional accessories’).
Prior to inserting the knife, always tighten one of the knife clamping screws of the knife holder to secure the knife holder in position!
Make sure not to misalign the knife when tightening the knife clamping screws as this will negatively affect sectioning results!
Leica SM2500
Always remove the knife from the knife holder when finishing work!
When working with the eccentric ring, NEVER try to orient the specimen - this will cause severe damage to the specimen orientation mechanism!
When knife holder A or C is combined with the declination blocks, the knife guard cannot be placed over the knife edge. Therefore, use extreme caution when working with this configuration
- wear cut-resistant gloves!
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2. Safety
Safety instructions: Cleaning, disinfection and maintenance
Only authorized service technicians may access the internal components of the instrument for service and repair!
Prior to any cleaning, disinfection or maintenance work, the instrument must be switched off and discon­nected from mains! Exception: Cleaning or disinfection procedures which require the sledge to be moved.
Remove both knife and specimen prior to any cleaning, disinfection or main­tenance work!
Clean the control panel only in standby mode or when the instrument is switched off.
Appropriate cleaners for non-var­nished metal parts (knife holders, base plates, specimen clamps): xylene substitutes, alcohol - apply with an only slightly moistened clean­ing cloth!
Use cleaners and disinfectants ac­cording to the safety instructions of the manufacturer and in compliance with the regulations applicable in your laboratory!
Wear cut-resistant safety gloves to re­move the knife (see chapter 8.1 ‘Addi­tional accessories’).
No liquids may enter the interior of the instrument during cleaning, disinfec­tion or maintenance!
Clean only with a slightly moistened cleaning cloth!
Disinfection only with disinfectants that allow application with a slightly moistened cloth!
Do not use any solvents (e.g. xylene, acetone), any cleaners containing solvents or scouring powders for cleaning!
Appropriate cleaners for the control unit / control panel and all painted sur­faces: mild laboratory detergent (slightly moistened cloth)!
Regularly check and empty the sec­tion waste trays located below the ex­tendible carrying handles.
Prior to exchanging any fuse, always switch the instrument off and unplug the mains plug. Replace defective fuses only with fuses of identical specification! Be sure to follow the instructions in chapter 7.2.2 (selecting the correct re­placement fuse for each fuse holder). After replacing the line fuses double­check whether the correct voltage se­lector setting is visible.
Users may carry out only the mainte­nance work described in chapter 7.2!
Any other maintenance work must be carried out by authorized Leica ser­vice technicians.
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Instruction manual V 1.3 – 10/2007
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3. Technical data

Microtome
Section thickness range ...........................................................0 μm to 1.000 μm, adjustable in 1-μm steps
Total horizontal specimen stroke .......................................................................................maximum 275 mm
Total vertical knife feed ........................................................................................................................... 70 mm
Knife retraction (during specimen return stroke) ...................................................................... 0 - 1,000 μm
Clearance angle adjustment ............................................................................................................. 0 ° to 17 °
Knife declination - fixed setting (declination blocks = optional accessory) ....................................... 45 °
Maximum specimen size (L x W x H) ........................................................................................ 25 x 20 x 7 cm
Specimen orientation (along x / y axis) ..................................................................... 4,8 ° along each axis
Specimen orientation (rotability) ................................................................................. approx. +/- 3 and 90 °
Sectioning speed ................................................................... 0.5 - 100 mm/s, adjustable in 0.1-mm steps
Return speed ................................................................... 0.5 - 100 mm/s, adjustable in 0.1-mm steps
Manual knife movement (slow / fast) ......................................................................... 37 mm/s and 74 mm/s
Manual specimen movement (slow / fast)................................................................. 37 mm/s and 74 mm/s
Electrical connections
Nominal voltage .............................................................................................................. 100 / 120 / 230 / 240 V
Nominal frequency ...................................................................................................................50 Hz and 60 Hz
Maximum power draw ......................................................................................................................... 1,400 VA
Main fuses, type MDA, Fa. Bussmann .............................................................................................. 2 x T10A
Protective class .................................................................................................................................................. I
Overvoltage installation category ...................................................................................................................II
Vacuum cleaner ........................................................................ 100 / 120 V - maximum power draw 500 VA
Vacuum cleaner ..................................................................... 230 / 240 V - maximum power draw 1,200 VA
Lamp .......................................................................................... 100 / 120 V - maximum power draw 100 W
Lamp .......................................................................................... 230 / 240 V - maximum power draw 200 W
Dimensions and weights
Microtome (H x W x L) ....................................................................................................... 250 x 390 x 750 mm
Control unit (H x W x D) ...................................................................................................... 220 x 385 x 510 mm
Required bench top space for microtome and control unit ................................................ 1,000 x 950 mm
Microtome ..................................................................................................................................... approx. 75 kg
Control unit .................................................................................................................................... approx. 23 kg
Leica SM2500
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4. Setup

4.1 Overview - instrument parts / functions

1.3 1.11.2 1 2.1 2.22
Fig. 3a
1.4
1. Leica SM2500 - Microtome
1.1 Knife holder system (not part of standard delivery)
1.2 Specimen holder system (not part of standard delivery)
1.3 Carrying handle, rear (extendible - not visible in Fig. 3a)
1.4 Carrying handle, front (extendible)
2. Leica SM2500 - control unit
2.1 Control unit
2.2 Emergency stop button
3. Foot switch (not visible in Fig. 3a ­see chapter 5.4.1 for details)
4.2 Site requirements
Bench top surface for microtome and control unit sized approx. 1,000 x 950 mm
Stable laboratory bench (total weight of microtome and control unit approx. 100 kg), exactly hori­zontal or horizontally adjustable.
At least 15 cm space on all sides of both instruments to ensure that air can circulate freely around the instruments.
Virtually vibration-free floor.
Stable ambient temperature between +18 °C and +35 °C.
Relative air humidity of maximum 60 %, non-condensing.
Do not place near sources of vibration, avoid exposure to direct sunlight and marked temperature fluctuations.
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Instruction manual V 1.3 – 10/2007
Page 15

4.2 Standard delivery Leica SM2500

1 Basic instrument
1 Separate control unit ............................................................................. 0405 42863
1 Connecting cable for control unit ........................................................ 0405 35685
1 Foot switch (0505 33888) 1 Set of power cords:
-1 Power cord “D” ............................................................................... 0411 13558
-1 Power cord “USA-C-J” .................................................................. 0411 13559
-1 Power cord “UK” ST/BU F-5A....................................................... 0411 27822
1 Set of adapter cables:
-2 Adapter cables “USA” ................................................................... 0411 36234
-2 Adpater cables “EU” ...................................................................... 0411 36235
1 Set of replacement fuses:
-2 x T 1.0 A ............................................................................................ 6943 01001
-2 x T 6.25A ........................................................................................... 6943 06251
-2 x T 10.0 A .......................................................................................... 6943 10001
1 Tool set:
-1 Allen key, size 2.5 ............................................................................ 0194 13195
-1 Allen key, size 3.0 ............................................................................ 0194 04764
-1 Allen key, size 4.0 ............................................................................ 0194 04782
-1 Allen key, size 5.0 ............................................................................ 0194 04760
-1 Allen key, size 8.0 ............................................................................ 0194 04792
-1 Torque wrench, size 32 .................................................................. 0383 11934
-1 Single-head wrench, size 13 ......................................................... 0330 11936
-1 Screw driver .................................................................................... 0170 12988
-1 Clamping device .............................................................................. 0383 32392
-1 Remote alarm jack 6.3 mm............................................................. 6844 01005
-1 Jack connector 3-contact .............................................................6844 01008
1 Brush ........................................................................................................ 0183 04477
1 Grease gun .............................................................................................. 0383 11896
1 Dust cover for microtome ..................................................................... 0212 11725
1 Dust cover for control unit .................................................................... 0212 36428
1 Rubber mat .............................................................................................. 0405 36405
1 Instruction manual for Leica SM2500 ................................................. 0701 35100
4. Setup
Specimen and knife holder systems are not included in the instrument standard delivery. They have to be ordered separately, in accordance with each user’s specific application(s). Mounting instructions for all accessories can be found in chapter 5.5 of this instruction manual.
Leica SM2500
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4. Setup

4.4 Unpacking, transport, installation

2
1
2
1
Fig. 4
3
4
Pull out the carrying handles (1) at the front and rear of the instrument.
Remove the upper housing parts (2) at the front and rear.
Secure the carrying handles (1) placing the white plastic blocks (3) on
Be sure to follow the unpacking instructions (attached to the out­side of the instrument shipping crate)!
When transporting the instrument, always lock the extendible carrying handles in place by attaching the transport safety de­vices (white plastic blocks)!
the telescopic bars (4).
Two persons are needed to lift and/or carry the instrument (instru­ment weight!). The instrument may only be transported in an upright position!
Once the instrument has been placed onto the bench, remove the two plastic blocks (3) from the telescopic bars (4) and put them back into their storage place in the instrument housing.
• Put the upper housing parts (2) back in place and push the carrying handles (1) inwards as far they will go.
Fig. 5
34
16
Prior to connecting the instrument to mains, verify whether the voltage setting is correct for the mains electricity in your lab (please refer to chapter 4.4.1 ‘Verifying and adjusting the voltage selector’)!
The instrument may not be operated in hazardous locations where risk of explosion exists!
Never remove or modify any of the safety devices installed on the instrument and/or accessories!
Fig. 6
Instruction manual V 1.3 – 10/2007
Page 17

4.5 Electrical connection

4.5.1 Verifying and adjusting the voltage selector

The voltage selector comes factory-adjusted to a setting of 230 V. Prior to connecting the instrument to mains, verify whether the voltage setting is correct for the mains electricity in your lab! Incorrect voltage selector settings can lead to severe damage to the instrument! Prior to adjusting the voltage selector, disconnect the instrument from mains!
Checking the voltage selector setting:
The voltage selector is located above the mains switch, on the rear left of the control unit.
Does the selected setting (1) correspond to the nominal voltage in your laboratory?
1
If yes: --> proceed to chapter 4.4.2 on the following page.
If no: --> the voltage selector must be changed to the correct setting as follows:
Insert a small screw driver into the notch at the lower end of the voltage selector housing and carefully remove the housing, using the screw­driver as a lever.
• Remove housing (2) together with fuses (3) and the voltage selector
342
1
Fig.7
block (4) (white color).
Remove the fuses.
Remove the voltage selector block and re-insert it so that the correct setting, corresponding to the mains voltage in your lab, is visible in win­dow (1). (Selectable voltage settings: 100, 120, 230 and 240 V).
Re-insert the fuses.
Re-insert the housing together with the voltage selector block and the fuses into the instrument and push slightly inwards until the voltage se­lector housing locks in place (audible click).
Verify once again, whether the setting that shows in window (1) is ap­propriate for the mains voltage in your lab.
4. Setup
Leica SM2500
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4. Setup

4.5.2 Installing power cord, accessory cords and connecting cable to the microtome

At the rear of the control unit, set up the following connections:
Remove the tape from the mains inlet for the power cord (8).
From the set of power cords delivered (the instrument is delivered with several, country-specific power cords), select the one that is appropriate for your lab; connect the power cord.
Fig. 8
Do not yet plug the power cord into the mains outlet.
Connect the remaining cables:
Fig. 9
Install the connecting cable (1) leading to the microtome and tighten the
132
5
674
8
fixing screws.
Connect the other end of cable (1) to the right side of the microtome and tighten the fixing screws.
• The connecting cable to the ultramiller (2) needs to be installed only when working with the Leica SP2600 --> see separate instruction manual Leica SP2600.
Connect photo camera (3) (optional).
Connect remote alarm (4) (optional).
Connect foot switch (5) (NOT optional).
18
The foot switch must be connected - otherwise the instrument will not be operational.
Connect the vacuum cleaner (6) (optional with Leica SM2500 - necessary when working with Leica SP2600).
Connect the lamp (7) (optional).
Instruction manual V 1.3 – 10/2007
Page 19

4.5.3 Connecting a remote alarm device or remote camera shutter

Connecting a remote alarm device:
A remote alarm device can be connected to the Leica SM2500 via a 6.3 mm­diameter jack connector (optional accessory).
• For details on how to connect a remote alarm device to the Leica
SM2500, please contact your local Leica office or the manufacturer Leica Biosystems Nussloch directly.
Connecting a camera shutter:
The Leica SM2500 offers the option to automatically trigger a photocamera shutter each time the specimen sledge reaches a preselected photo posi­tion.
The connecting cable leading from the camera to the Leica SM2500 must be configured individually, depending on the type of camera used.
For on this option, please contact your local Leica office or the manufac-
turer Leica Biosystems Nussloch directly.
4. Setup
Leica SM2500
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5. Operation

5.1 Overview of chapter 5 ‘Operation’
Chapter 5 ‘Operation’ is structured as follows:
Chapter 5.1 - Structural description of chapter 5. Chapter 5.2 - Switching the instrument on. Chapter 5.3 - Overview - control panel. Chapter 5.4 - Detailed description of each individual control element
Chapter 5.5 - Mounting the accessories (specimen holders,
Chapter 5.6 - Working with the instrument:
Chapter 5.7 - Switching the instrument off
Initial operation:
The first time you operate the instrument we recommend you work through chapters 5.1, 5.2, 5.4 and 5.5, in that order.
Pay particular attention to the safety instructions. Once you are familiar with the control panel functions and know how to in-
stall the accessories, proceed to chapters 5.6 / 5.7.
(controls numbered from 1 to 62; control panel divided into functional areas 1 to 5).
knife holders, knives).
short description of a typical application, including references to detailed descriptions of individual steps given in other chapters.
20
Daily routine work:
The control panel overview (chapter 5.3 on pages 20/21) will be a useful tool for your daily routine work.
Instruction manual V 1.3 – 10/2007
Page 21

5.2 Switching the instrument on

Switch on the mains switch (0/I) located at the rear side of the control panel (On = switch position ‘I’):
All control elements on the control unit will emit light for about 2 secs. Next, the software version is displayed in the section counter (no. 44,
upper display in functional area 4 - chapter 5.4.12).
5. Operation
Caution: Did you verify whether the voltage selector is set correctly? ­If not, please refer to chapter 4.4.1! Incorrect voltage selector settings can lead to severe damage to the instrument!
The instrument may be connected to mains only with one of the power cords supplied and only to grounded sockets! The instrument may not be operated in hazardous locations where risk of explosion exists! Extreme temperature fluctuations and high air humidity (transport, storage) may cause condensation inside the instrument. In this case, wait at least two hours before switching the instru­ment on. Failure to comply with this waiting period may cause severe dam­age to the instrument!
Leica SM2500
Fig. 10
After switching on the mains switch, the instrument will go to standby mode (mains voltage is supplied to the control unit, the control panel is, however, deactivated).
To activate the control panel, press and hold the STANDBY button for approx. 3 secs:
the control elements are activated.
Control parameters stored prior to switching the instrument off will still be available when the instrument is switched on again.
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5. Operation

5.3 Overview control panel - by functional areas

Area 3 - includes the controls for the supplementary instruments
(41) Rotational speed of miller (RPM)
and accessories that can be attached to the Leica SM 2500, (ultramilling attachment, vacuum cleaner, lamp).
(33/34) Lamp On/Off
Fig. 11
(40, 39) to set rotational
speed of miller
Area 4 contains two displays:
-Section counter (total number of sections / milling strokes)
-Section thickness totalizer (total thickness of sections / milling strokes)
Contains two reset buttons, one for each display.
Area 5.1 contains all display elements and
control buttons for manual movement of knife / miller (operational in manual mode of operation ‘MAN’).
(48) - Knife/miller fast upwards
(49) - Knife/miller slow upwards
(50) - Knife/miller slow downwards
(51) - Knife/miller fast downwards
(47) - LED
(52) - LED
(40) (39)
(56)
(57)
(58)
(59)
(60) - LED
(35/36) Vacuum cleaner On/Off
(37/38) Miller rotation On/Off
(44) Section / milling stroke
counter
(42) Reset for counter (44)
(43) Reset for counter (45)
(45/46)Section thickness /
milling stroke thickness totalizer
(53) - LED
(54) - LED
(55) - LED
(61/62) - Sectioning / milling
stroke limit
(56 - 59) - Manual sledge
movement (forward/backward / fast / slow)
Area 5.2 ­contains the display and control elements for manual movement of the specimen (in manual mode of operation ‘MAN’) and for limiting the sectioning / milling stroke length (‘Sectioning window’).
Fig. 12
22
Instruction manual V 1.3 – 10/2007
Page 23
5. Operation
Area 1 - to switch from standby mode to active mode (control panel activated) and back.
Emergency stop button, stops all instrument functions immediately when pressed.
Area 2.1­to select a mode of operation via button (5): manual mode ‘MAN’ (6) automatic mode ‘AUTO’ (7) and program mode ‘PROG’ (8)
Area 2.3­to select a sectioning / milling mode via button (15): continuous mode (16) single mode (17) or step-by-step mode (18)
Area 2.5 ­to select a stop position (24/25) or photo position (27/28), where the sledge will stop.
(23) Selector button
‘Stop position’
(26) Selector button
‘Photo position’
Area 2.6 ­contains the buttons to start and stop sectioning / milling and the LEDs that indicate sectioning / milling status.
(5) (6)
(7) (8)
(15)
(16) (17) (18)
(24)
(25)
(27)
(28)
(1) LED
(2) Standby On/Off (3) LED (4) Emergency stop button
Area 2.2­selection (10) and indication (14) of section/milling
(11)
(12) (13) (10)
(14)
(22) (19)
(20)
(21)
thickness (11), preselected number of sections / milling strokes (12) (in mode of operation PROG only) and knife retraction (13).
Area 2.4­to select (19) and indicate (22) sectioning / milling speed (20) and return stroke speed (21) of the sledge
(9)
Rotary-type selector switch (9) for entering parameters (11), (12) and( 13) in area 2.2 as well as for entering parameters (20) and (21) in area 2.4.
Leica SM2500
(29) (30) (32) (31)
To start: press (29) and (30) simultaneously To stop: press either (29) or (30)
Fig. 13
23
Page 24
5. Operation

5.4 Description of each individual control element

5.4.1 Foot switch

The foot switch has two different functions:
1 Start / stop sectioning / milling.
The foot switch can be used as an alternative to the RUN STOP + RUN ENABLE buttons (--> see area 2.6, chapter 5.4.10) so start / stop section­ing / milling.
- To start, lightly press the foot switch.
- To stop, once again lightly press the foot switch.
Fig. 14
24
2 Emergency stop.
In addition to the emergency stop button (see area 1, chapter 5.4.2), the emergency stop can be activated via the foot switch:
- Press foot switch once forcefully: emergency stop function is activated. POWER (green) and E-STOP (red) on the control emit light.
As soon as the foot switch is released the emergency lock is auto­matically unlocked and the instrument can be restarted. E-STOP (red) on the control panel goes off.
Instruction manual V 1.3 – 10/2007
Page 25

5.4.2 Area 1 - Standby on/off; emergency stop

1
5. Operation
234
1 POWER (LED)
Emits light (green) when the instrument mains switch in ON.
2 STANDBY (button)
Pressing this button switches the standby mode on/off. Press and hold button for approx. 3 secs to switch from standby mode to
active mode and vice versa.
3 E-STOP (LED)
Emits light (red), when the emergency stop button has been pressed or if the foot switch is not connected at all / not properly connected.
4 Emergency stop (button)
Pressing this button immediately stops all instrument functions and movements. LEDs ‘E-STOP’ and ‘POWER’ start emitting light.
All settings (modes of operation, set values and counter readings) remain stored and can be reaccessed when the instrument is switched back on.
Unlocking the emergency stop / switching the instrument back on:
1. Turn the red button in direction of the arrow until it unlocks: LED E-STOP goes off.
2 Select a mode of operation (MAN / AUTO / PROG) --> see area 2.1,
chapter 5.4.3.
3. If mode of operation = AUTO: select also a sectioning / milling mode
--> see area 2.3, chapter 5.4.6.
Fig. 15
Leica SM2500
25
Page 26
5. Operation

5.4.3 Area 2.1 - Modes of operation

5 RUN MODE (button)
Press RUN MODE to select one of the operation modes below.
6 MAN (LED)
6
8
7
5
Fig. 16
Emits light, when manual mode of operation MAN has been selected. In MAN mode, area 5 is activated:
- Knife (or miller) and sledge can be moved manually (e.g. for bring­ing the knife closer to the specimen).
- Sectioning / milling stroke limit (‘window’) can be defined.
- Section / milling thickness, retraction (area 2.2), sectioning / milling speed as well as sledge return speed (area 2.4) can be selected.
7 AUTO (LED)
Emits light, when automatic mode of operation AUTO has been selected.
- Sectioning / milling mode (area 2.3) can be selected.
- Parameter selection in areas 2.2 and 2.4 as in MAN.
- Pressing RUN/STOP + RUN/ENABLE (or lightly pressing the foot switch) in AUTO mode starts sectioning / milling (push to start / push to stop).
26
8 PROG (LED)
Emits light, when program mode of operation PROG has been selected.
- Parameter selection in areas 2.2 and 2.4 as in AUTO and MAN. Additional features of PROG mode: Preselection of a specific number ‘n’ of sections / milling strokes to be done (LED (12) in area 2.2).
- Sectioning / milling in PROG mode (press RUN/STOP + RUN/EN- ABLE or activate foot switch): the preselected number of sections / milling strokes is carried out as per the parameters defined (number of sections, section thickness etc.); then the sledge stops in the defined stop position (area 2.5) and an acoustic signal indicates the end of the sectioning program.
Parameters selected always remain stored until new parameters are entered (also when the instrument mains switch (0/I) is switched off).
Instruction manual V 1.3 – 10/2007
Page 27

5.4.4 Rotary-type selector switch in area 2

9 The parameters in areas 2.2 and 2.4 are entered via the rotary selector
switch no. 9:
- Sectioning / milling thickness in μm
9
Fig. 17
(section / milling feed via knife / miller)
- Preselection of number of sections / milling strokes (to be carried out in ‘PROG’ mode)
- Retraction in μm (= knife / miller retraction during specimen return stroke)
- Sledge speed - forward (= sectioning / milling speed)
- Sledge speed - backward (= return stroke speed)
5. Operation
Leica SM2500
27
Page 28
5. Operation

5.4.5 Area 2.2 - Section/milling thickness, section/milling stroke preselection, retraction

12
14
11
10
13
10 SET (button)
Press SET to activate indication of FEED, n or RETRACT: LED starts emit­ting light. For entering new parameters with selector switch (9), press and hold SET until the corresponding LED starts blinking.
11 FEED (LED)
When LED FEED is blinking: rotate switch (9) to enter a section/milling thick­ness from 0 μm (knife / miller feed off) to 1,000 μm (selectable in 1-μm steps).
12 n (LED)
When LED n is blinking and PROG mode is activated: rotate switch (9) to preselect the number of sections / milling strokes to be carried out (from 1 to 1,000).
13 RETRACT (LED)
When LED RETRACT is blinking: rotate switch (9) to enter a setting between 0 μm (retraction switched off) to 1,000 μm (selectable in 1-μm steps).
In AUTO and PROG mode, the knife / miller is lifted away from the specimen surface at the end of each section / milling stroke (retrac­tion). The knife / miller then stays in retraction position over the entire length of the sledge return stroke. When the retraction feature is activated (retraction value >0), RETRACT in area 5.2 emits light while the sledge is in return stroke. When the sledge reaches the front end position, the knife / miller is lowered back towards the specimen by the selected retraction value.
Fig. 18
28
14 Indication of section/milling thickness, section/milling stroke
preselection or retraction parameters that have been entered via rotary switch (9). - All parameters are displayed flush right.
All parameters are stored automatically upon entry (no confirma­tion necessary) and remain stored until new settings are entered. The LEDs stop blinking automatically after a few seconds.
Instruction manual V 1.3 – 10/2007
Page 29

5.4.6 Area 2.3 - Sectioning / milling modes

To be able to select a sectioning / milling mode, mode of operation AUTO (area 2.1) must be activated first.
16
15 CUT MODE (button)
Press button (15) to select a sectioning / milling mode.
16 CONT (LED)
17
18
Fig. 19
15
Emits light, when continuous mode of operation ‘CONT’ has been selected: In continuous mode, sectioning / milling starts when RUN STOP + RUN
ENABLE or the foot switch are pressed and continues until RUN STOP or RUN ENABLE or the foot switch are pressed once again.
17 SINGLE (LED)
Emits light, when single-stroke mode of operation ‘SINGLE’ has been se­lected:
When RUN STOP + RUN ENABLE or the foot switch are pressed in single-stroke mode of operation, the instrument carries out one section / milling stroke and then stops in the preselected stop position.
5. Operation
Leica SM2500
18 STEP (LED)
Emits light, when step-by-step mode of operation ‘STEP’ has been selected: in step-by-step mode, the instrument will section / mill only as long as RUN STOP + RUN ENABLE are pressed and held.
29
Page 30
5. Operation

5.4.7 Area 2.4 - Sledge speed

20
22
Fig. 20
19
21
19 SPEED (button)
To set the cutting or return speed, press and hold the SPEED button until the corresponding LED blinks. Enter speed using the rotary button (9).
20 CUT (LED)
Press and hold SPEED until the LED CUT starts blinking. Then enter the desired sectioning / milling speed of the sledge via selector switch (9) (selectable speeds: 0.5 - 100 mm/sec, in 0.1-mm steps).
21 RETURN (LED)
Press and hold SPEED until the LED RETURN starts blinking. Then enter the desired return stroke speed of the sledge via selector switch (9) (se­lectable speeds: 0.5 - 100 mm/sec, in 0.1-mm steps).
22 Speed indication
Indicates sledge speed during sectioning / milling stroke and return stroke.
30
CUT and RETURN can be changed while sectioning / milling is in progress. All settings entered are automatically stored upon entry. No additional confirmation is required. The LEDs stop blinking automatically after a few seconds.
Instruction manual V 1.3 – 10/2007
Page 31

5.4.8 Area 2.5 - Setting the stop or photo position

23 STOP POS (button)
Press STOP POS to select one of the two stop positions available.
23
24 FRONT (LED)
24
25 27
28
Fig. 21
26
Starts emitting light when the sledge stops in the front stop position.
25 REAR (LED)
Starts emitting light when the sledge stops in the rear stop position.
26 Photo position (button)
Press the Photo position button to select one of the two photo positions available.
27 PHOTO F (LED)
Starts emitting light when the sledge stops in the front photo position.
5. Operation
Leica SM2500
28 PHOTO R (LED)
Starts emitting light when the sledge stops in the rear photo position.
You may either select a stop position or a photo position. When a photo position has been selected, there cannot be se­lected a stop position and vice versa.
31
Page 32
5. Operation

5.4.9 Area 2.5 - Changing the dwelling and triggering time in photo position

Changing the dwelling time in photo position:
- While switching on the instrument mains switch (rear of control panel) simultaneously press and hold the STOP POS button (in area
2.5) until the instrument initialization phase has been completed.
- The ‘rpm’ display (area 3) will read the currently selected dwelling time in seconds.
- Press the ‘+’ or ‘-’ buttons below the ‘rpm’ display to select a new dwelling time (time the sledge will remain in photo position before the photograph is taken) from 0 secs to 25 secs (in 100-ms steps). (A minus sign will be displayed in while you are entering your selection).
Fig. 22
- To store the selected setting, press STANDBY (area 1): The ‘rpm’ indication in area 3 goes off - the selected value is stored.
∑∑
n (area 4 - see chapter 5.4.12)
∑∑
Fig. 23
32
Changing the triggering time (= exposure time):
- Same procedure as when changing the dwelling time. Instead of STOP POS, press and hold the Photo position button (in area 2.5) while switching on the instrument mains switch.
- Just like the dwelling time, a triggering time from 0 secs to 25 secs can be selected. The figure ‘1’ will be indicated in display
∑∑
n (area 4 - see chapter
∑∑
5.4.12) while you enter your selection.
- To store the selected setting, press STANDBY (area 1): as soon as the ‘rpm’ indication in area 3 goes off the selected value is stored.
Instruction manual V 1.3 – 10/2007
Page 33

5.4.10 Area 2.6 - Start / stop sectioning / milling

5. Operation
31
32
Start:
29
30
Fig. 24
29 RUN STOP (button)
30 RUN ENABLE (button)
31 RUN (LED)
32 STOP (LED)
Press RUN STOP and RUN ENABLE simultaneously:
+
- sectioning / milling is activated. LED RUN will be emitting light while sectioning / milling is in progress.
Stop:
Press either RUN STOP or RUN ENABLE:
- sectioning / milling movement is stopped. LED STOP will start emitting light, once sectioning / milling has
or
stopped.
After you have pressed RUN STOP or RUN ENABLE or the foot switch, the instrument will complete the sectioning/milling stroke in progress before stopping in the stop (or photo) position se­lected.
Leica SM2500
Instead of using the RUN STOP / RUN ENABLE buttons, sectioning / milling may be started / stopped as well via the foot switch (see chapter 5.4.1 ‘Foot switch’).
33
Page 34
5. Operation

5.4.11 Area 3 - Operating optional supplementary instruments

41
34
33
36
40
33 Lamp (button)
Switching the lamp on/off (lamp is not part of standard delivery).
34 LED - starts emitting light when lamp button is switched on.
35 Vacuum cleaner (button)
Switching the vacuum cleaner on/off (vacuum cleaner is not part of standard delivery).
36 LED - starts emitting light when vacuum cleaner button is switched on.
37 Miller (button)
Switching the rotational movement of the ultramilling attachment Leica SP2600 on/off (separate supplementary instrument - not part of Leica SM 2500 standard delivery).
38 LED - starts emitting light when ultramiller button is switched on.
39 ‘+’ button (rotational speed of miller in RPM)
Press ‘+’ to increase the rotational speed of the ultramiller in steps of 100 (from 500 to 3.000 RPM).
40 ‘-’ button (rotational speed of miller in RPM)
Press ‘-’ to decrease the rotational speed of the ultramiller in steps of 100 (from 500 to 3.000 RPM).
39
37
38
35
Fig. 25
34
41 Rotational speed of miller (display)
Shows the currently selected rotational speed of the ultramiller.
Instruction manual V 1.3 – 10/2007
Page 35
5. Operation

5.4.12 Area 4 - Section/milling stroke counter and thickness totalizer and reset buttons

Vertical position < 0
Knife
‘0’
Vertical position > 0
2345678901
2345678901
2345678901
2345678901
2345678901
Fig. 27
Specimen
block
44
45
42
43
46
Fig. 26
42 CLEAR (button) - reset button for the section / milling stroke counter.
Pressing CLEAR resets counter (44) to ‘0’.
∑∑
44
n Section / milling stroke counter.
∑∑
Displays the total number of sections (or milling strokes) made since the last reset.
43 CLEAR (button) - reset button for the section / milling thickness totalizer.
Pressing CLEAR resets counter (45) to ‘0’.
By resetting the totalizer (45) to ‘0’ you can define any level of the specimen as ‘zero’ thickness (e.g. after a specimen has been milled until it is exactly horizontal). This feature gives you exact control on how much specimen mate­rial (thickness) is removed from a defined point ‘zero’ onwards. In addition, it enables you to go back to exactly the same vertical knife / miller position after a manual upward movement (see area
5.1, chapter 5.4.13) of the knife / miller, i.e. after moving to a verti­cal position of knife / miller < 0.
45 Display
∑∑
μm - section / milling thickness totalizer.
∑∑
Shows the total thickness of all sections / milling strokes carried out since the last reset.
46 LED - indicates vertical position of knife/miller, relative to the selected
‘zero’ point, during manual movement (see area 5.1, chapter 5.4.13):
Knife / miller in vertical position > 0 = knife / miller moving from ‘zero’ downwards, towards the speci­men surface: LED does not emit light.
Knife / miller in vertical position < 0 = knife / miller moving from ‘zero’ upwards, away from the speci­men surface: LED emitting light.
Leica SM2500
35
Page 36
5. Operation

5.4.13 Area 5.1 - Manual movement of knife / miller

47 UP (LED)
Flashing during fast upward movement of the knife / miller. LED stays lit as soon as the upper end position has been reached.
47
48
49
50
51
52
Fig. 28
48 Knife / miller fast upwards (button)
Press button (48) for fast upward movement of the knife / miller: The knife / miller will keep moving upwards until reaching the upper limit
stop or until button (48) is pressed once again.
49 Knife / miller slow upwards (button)
Press button (49) for slow upward movement of the knife / miller. Knife / miller will move upwards only as long as button (49) is held.
50 Knife / miller slow downwards (button)
Press button (50) for slow downward movement of the knife / miller. Knife / miller will move downwards only as long as button (50) is held.
51 Knife / miller fast downwards (button)
Press button (51) for fast downward movement of the knife / miller. Knife / miller will move downwards only as long as button (51) is held.
52 DOWN (LED)
Starts emitting light, when knife / miller has reached the lower limit stop position.
36
Instruction manual V 1.3 – 10/2007
Page 37

5.4.14 Area 5.2 - Manual movement of the sledge

53 MAN (LED)
Lit in manual mode of operation. - LED MAN must be on in order to be able
57
55
56 53
58 60 59 61
Fig. 29
62
to access/activate the control buttons in area 5.
54 RETRACT (LED)
Lit (also when retraction = 0) while the specimen sledge is in return stroke -
54
see also area 2.2 ‘Setting the retraction parameters’.
55 CUT (LED)
Starts emitting light when the sledge reaches the rear limit position (sledge being moved via buttons (56/57)).
56 Sledge fast forward (button)
Sledge moves only while the button is being held.
57 Sledge slow forward (button)
Sledge moves only while the button is being held.
58 Sledge slow backward (button)
Sledge moves only while the button is being held.
59 Sledge fast backward (button)
Sledge moves only while the button is being held.
60 RETURN (LED)
Starts emitting light when the sledge reaches the front limit position (sledge being moved via buttons (58/59)).
61 Sectioning/milling window (limitation of the sectioning/milling stroke)
Setting the sectioning window:
- Move the sledge to the first limit position you wish to set (use buttons 56 - 59).
- Press and hold button (61) until LED (62) starts flashing - then move the sledge on to the second desired limit position.
- Press and hold button (61) once again, until LED (62) stays lit, thus indi­cating that both positions have been stored.
Deleting the sectioning window:
Press and hold button (61) just once until the LED starts flashing - the instrument will return to maximum sledge stroke length as soon as the LED stops flashing after a few seconds.
62 LED ‘Sectioning / milling window’ in button (61):
- Flashes - while the sectioning window is being set.
- Stays lit - when a sectioning window has been defined.
- Off - when no sectioning window has been defined.
5. Operation
Leica SM2500
The sectioning window settings cannot be changed while section­ing/milling is in progress; changes are possible only in MAN mode.
37
Page 38
5. Operation
Fig. 30
Fig. 31
5.5 Mounting the accessories
Always clamp the specimen first and the knife second!

5.5.1 Mounting the base plates

Base plate with vise and base plate with dovetail guide are mounted the same way. Shown here is the base plate with vise.
Preparing the base plate
Insert the clamping device (1) into the two notches on the underside of the base plate (base plate and clamping device are delivered separately).
Turn approx. 45° while pressing slightly down-
1
1
wards - until the clamping device locks in place.
The clamping device can be turned 90° to a sec­ond locking position, so that the base plate can be mounted onto the sledge in two positions:
with the dovetail guide or vise at right angles to the sledge direction;
with the dovetail guide or vise in longitudinal direction.
To turn, press the clamping device slightly downwards, turn 90° and let go - it will lock in place automatically.
Fig. 33
38
Fig. 32
1
Instruction manual V 1.3 – 10/2007
Page 39
Fig. 34
5. Operation
Mounting the base plate onto the sledge
Remove the lid (1) from the sledge.
1
3
Position the sledge about 3 cm away from the front limit position: In front, there must be enough space to easily place torque wrench (8) on eccentric bush (2). The microtome spindle must not be visible in opening (3).
Pull eccentric bush (2) forward until the red ring becomes visible and the red line is on top.
If desired, insert eccentric ring (4) (plane side facing upwards!).
Fig. 34a
Eccentric ring - optional!
Fig. 36
Fig. 37
2
4
Fig. 35
The eccentric ring blocks the orienta­tion mechanism to ensure the section­ing plane is not altered.
Caution! When working with the eccentric ring, NEVER attempt to orient the specimen, as this would severely damage the specimen orientation mechanism! - See also chapt. 5 ‘Specimen orientation’.
5
Insert clamping device (6) of base plate (5) ver-
6
7
tically into opening (3).
Lock centering pin (7) into place (tilt slightly).
Push eccentric bush (2) inwards as far as it will go (red line must be on top).
Turn eccentric bush (2) clockwise until the
2
green line is on top.
Place torque wrench (8) (size 32) on the eccen­tric bush (with the arrow pointing to the right).
Turn torque wrench clockwise until a slight click can be heard and felt (= tightening torque
8
of 45 Nm).
DO NOT TURN ANY FURTHER, to pre­vent the thread of the eccentric bush from being stripped!
Leica SM2500
39
Page 40
5. Operation

5.5.2 Clamping specimens or specimen stages in the base plate with vise clamp

In the base plate with vise you can ei­ther directly clamp specimen blocks or specimen stages holding specimen embeddings.
Fig. 38
2
Clamping square specimen blocks
The vise is equipped with two clamping jaws: a fixed jaw (1) and a movable jaw (2) the position of which can be changed. Both have a V-notch on one side.
The fixed jaw (1) remains in its standard posi-
1
5
tion (V-notch facing outwards, i.e. away from the specimen).
Insert the movable jaw (2) in a position that is
appropriate for the specimen size to be clamped, also with the V-notch facing out­wards (away from the specimen).
5
3
4
Insert the specimen block.
Tighten the movable clamping jaw via clamp-
ing screw (3), using an Allen key, size 8 or a single-head wrench, size 13.
Important - for square as well as for cylindrical specimen blocks: When the specimen block is tightly clamped, the front edge of block (4) must not have moved beyond the two lateral markings (5).
40
Instruction manual V 1.3 – 10/2007
Page 41
1
Fig. 39
5. Operation
Clamping cylinder-shaped specimen blocks
2
6
7
5
7
35 4
Remove screws (6) holding the fixed clamp­ing jaw (1) (Allen key, size 5).
Turn the jaw and re-insert with the V-notch facing inwards, towards the specimen.
Re-insert and tighten screws (6) of clamping jaw (1).
Insert the movable jaw, (2) in a position that is appropriate for the specimen size to be clamped and with the V-notch facing inwards (towards the specimen).
Tighten the movable jaw via clamping screw (3), using an Allen key size 8 or a single-head wrench, size 13.
Important - for square as well as for cylindrical specimen blocks: When the specimen block is tightly clamped, the front edge of block (4) must not have moved beyond the two lateral markings (5).
8
9
Leica SM2500
Fig. 40
Fig. 41
Clamping specimen stages
The fixed clamping jaw (1) remains in standard position (V-notch facing outwards, i.e. away from the specimen stage).
All specimen stage sizes (plastic and metal spec­imen stages) have a clamping block of identical size and are clamped as follows:
Insert the movable jaw (2) with its front edge (i.e. the edge facing the specimen) flush with marking (7).
Insert the specimen stage (e.g. 8, 9).
Tighten the movable jaw via clamping screw (3), using an Allen key size 8 or a single-head wrench, size 13.
41
Page 42
5. Operation
Fig. 42
1
2
Fig. 43
Embedding frame for specimen stages
The embedding frame (Fig. 42) is adjustable in size, so that it fits all specimen stage sizes avail­able. It is fitted onto the specimen stage for sub­sequent specimen embedding in paraffin or cel­loidin.

5.5.3 Mountable jaws for vise

For sectioning very high specimens, there are two mountable jaws available, which can be in-
1
2
serted into bores (1) and (2).
The mountable jaws are part of the standard delivery ‘knife holder B’. Their use is required with knife holder B, as with knife holder B the knife edge is located at a considerable dis­tance from the specimen surface.
KNIFE
2
Specimen clamp 44 x 58 mm mounted onto the dovetail
1
guide located at right angles to the sledge direction.
42
42
Fig. 45

5.5.4 Mounting specimen holders onto the base plate with dovetail guide

Mounting the specimen clamp 44 x 58 mm with the dovetail guide located at right angles to the direction of the sledge movement
Slide the clamp onto the dovetail guide, so that
the three clamping screws (1) are located in front (if necessary, relocate the three clamp­ing screws - see next page).
With the Allen key, size 4, tighten the two Allen
screws (2) located at the rear side of the clamp.
Insert the specimen block and clamp the 3
clamping screws (1) with an Allen key, size 4.
Instruction manual V 1.3 – 10/2007
Page 43
KNIFE
2
1
Specimen clamp 44 x 58 mm mounted correctly onto the dovetail guide installed in longitudinal direction
5
Specimen clamp 44 x 58 mm - with clamping screws located correctly for installation on dovetail guide located in longitudinal direction
4 3
Fig. 45
4
3
1
Fig. 46
5. Operation
Mounting the specimen clamp 44 x 58 mm with the dovetail guide located in longitudinal direc­tion
Slide the clamp onto the dovetail guide so that the three clamping screws (1) are located in front (if necessary, relocate the three clamp­ing screws - see below).
With the Allen key, size 4, tighten the two Allen screws (2) located on the left (2).
• Insert the specimen block and tighten the three clamping screws (1) with the Allen key, size 4.
Relocating the clamping screws
Remove the two outer clamping screws (1) completely.
Use the miniature Allen key (size 1.5 - deliv­ered together with clamp) to open Allen screw (3).
Remove pressure plate (4).
Unscrew center clamping screw (1).
Insert center clamping screw (1) into the bore (5) from the outside inwards.
Insert pressure plate (4) and slide onto center clamping screw.
Fasten Allen screw (3) just slightly.
Re-insert the outer screws (1).
Leica SM2500
43
Page 44
4
4
5
5
5
5. Operation
234
234
wrong
Fig. 47
234
3
Fig. 48 Fig. 49
Round specimen holder ­clamping lever released
correct
Round specimen holder ­clamping lever tightened
Clamping the round specimen holder
Prior to sliding the holder onto the dovetail
23
23
1
guide turn clamping lever (1) until the edge of the clamping lever (1) is parallel to the edge of the clamp (2) (Fig. 47 on the left shows under-
2
side of round specimen holder).
Slide the round specimen holder (3) onto the dovetail guide (Fig. 48).
Clamp the lever (Fig. 49).
If the clamping lever is not yet tightened once turned backwards as far as it will go, it has to be relocated:
Pull the lever outwards and turn it clockwise into the desired position.
Let go of the lever - it will automatically lock in place in the position selected.
Clamp the lever once again.
Clamping specimen blocks in the round speci­men holder
The round specimen holder comes with three in­serts of 6, 15 and 25 mm diameter.
To clamp one of the inserts (2, 3 or 4) remove clamping ring (1) turning it counterclockwise.
Place insert (2), (3) or (4) into clamping ring (1), place the clamping ring onto the specimen holder and fasten it (turn clockwise).
To insert a specimen, turn clamping ring (1) counterclockwise, until the specimen can be inserted.
Insert the specimen (5) and rotate clamping ring (1) clockwise to clamp.
To rotate a specimen that is already clamped, insert pin (6) into bore (7) and turn counter-
clockwise.
Rotate the specimen into the desired position.
Fig. 50
To retighten, insert pin (6) into bore (7) once again and turn clockwise until tight.
44
Instruction manual V 1.3 – 10/2007
Page 45
Fig. 51
4
Fig. 52
5. Operation

5.5.5 Specimen orientation

Caution:
1
2
Releasing the base plate:
Place torque wrench (1) onto eccentric bush (2) (arrow on the wrench must point to the right).
Turn the torque wrench counterclockwise,
4
until the eccentric bush becomes loose.
Turn knurled knob (3) (marked L/R in Fig. 52)
on the left of the eccentric bush for adjustment along the x-axis.
Turn knurled knob (4) (marked H/V) in Fig. 52)
on the right of the eccentric bush for adjust­ment along the y-axis.
Once the specimen has been oriented, place
the torque wrench (1) back onto the eccentric bush (2) (arrow on wrench pointing to the right) and turn clockwise until a slight click can be heard and felt (= tightening torque of 45 Nm).
When working with the eccentric ring, NEVER attempt to orient the specimen, as this would severely damage the specimen orientation mechanism!
Fig. 53
Leica SM2500
DO NOT TURN ANY FURTHER, to pre­vent the thread of the eccentric bush from being stripped!
1
2
45
Page 46
5. Operation

5.5.6 Mounting the knife holders

Handle microtome knives and blades with utmost care. Knife and blade edges are extremely sharp and can cause severe injuries!
Wear cut-resistant safety gloves when inserting / removing the knife (see also chapter 8.1 ‘Additional accessories’).
Never leave knives / disposable blades or knife holders with a knife / disposable blade inserted lying around!
Always store knives in the knife case when not in use!
Never place a knife on a bench with the edge facing upwards!
Never try to catch a falling knife or disposable blade!
Always clamp the specimen first and the knife second!

5.5.7 Inserting knives into the holders

46
For a detailed overview on which types of knives are appropriate for which knife holder, please refer also to chapter 8.2 on page 66.
The knives are inserted into the different knife holders as follows:
Knife holder A:
Clamp the knives with the writing facing upwards, i.e. with the wedge angle pointing downward, towards the specimen surface.
Knife holder C:
Clamp the knives with the writing facing upwards.
Knife holder B:
c-profile knives may be clamped with either surface facing upwards. b-profile knives must be clamped with the facet pointing backwards when
inserting the knife, i.e. pointing away from the specimen once the knife has been turned into sectioning position.
Instruction manual V 1.3 – 10/2007
Page 47
3
Fig. 54
5. Operation

5.5.8 Mounting knife holder A

6
4
6
4
5
7
5
3
2
1
8
Knife holder A consists of 3 components: Two clamping blocks that hold the knife holder
on the microtome bearing blocks and the knife holder itself.
Preassemble the three components prior to
mounting them onto the microtome: Slide the clamping blocks (1, 2) onto the pins
(3) of the knife holder. Block 1 (bearing the clearance angle scale) must be on the left, block (2) on the right. The slot in each clamping block must point backwards.
Secure the knife holder with slotted screws (4).
The bearing blocks of the microtome supporting the knife holder must be clean. - Clean them, if necessary. Place the preassembled knife holder onto the bearing blocks from above. Do not slide the knife holder onto the bearing blocks to prevent the bearing surface from getting scratched.
Leica SM2500
Place the knife holder onto the bearing blocks.
The pin on the underside of each clamping block must be fit into the bore in each bearing block.
The knife holder is secured with a total of 8 clamping screws: each clamping block is held down by two front (5) and two rear screws (6).
With an Allen key, size 8, lightly tighten
all 8 clamping screws (tighten them alternately to prevent misalignment).
Next, the 4 rear screws (6) have to be loos­ened again.
Tightening first all 8 screws followed by releasing the 4 rear screws is im­portant to prevent the knife holder from being misaligned!
47
Page 48
5. Operation
Fig. 55
Fig. 56
Turn the knife holder upwards into the correct position for inserting the knife (--> see Fig. 55).
If the knife holder cannot be turned, it has been misaligned. - In that case, loosen all 8 clamping screws and re-
9
tighten as described on page 45.
Re-tighten at least one of the 4 rear clamping screws (6) to secure the knife holder in that position.
Prior to inserting the knife, always tighten one of the rear clamping screws to secure the knife holder in position!
Put on cut-resistant gloves (see also chapter. 8.1 ‘Additional accessories’).
Insert the knife: The knife back (base of knife) must fit against
the bearing surface inside the knife holder. The wedge angle (9) must point downwards.
Tighten the 6 knife clamping screws (7, Fig. 54) (Allen key size 8), starting with the center screws and working your way outwards and from side to side to prevent any misalignment of the knife.
When clamping a short knife, only tighten as many screws as the knife is actually wide.
48
Make sure not to misalign the knife when tightening the knife clamping screws (7) as this will negatively af­fect sectioning results!
Place the knife guard over the knife edge.
Instruction manual V 1.3 – 10/2007
Page 49
Fig. 57
5. Operation
Loosen the rear clamping screw (6).
Turn the knife holder into the desired clear­ance angle position.
Setting ‘0’ on the clearance angle scale (10) corresponds to an actual clearance angle of 5°. Each scale graduation corresponds to an angle in­crement of 2°.
10
Hold the knife holder in that position and re­tighten one of the 4 rear clamping screws (6) to secure the clearance angle setting.
Let go of the knife holder and tighten all re­maining clamping screws (6) alternately.
Leica SM2500
49
Page 50
5. Operation
4
Fig. 58
1
5
6

5.5.9 Mounting knife holder B

Knife holder B consists of four components:
4
2
6
3
6
7
2 cylindrical holders (3) with integrated knife guards and 2 clamping blocks (1, 2) fixing the knife holder to the microtome.
1. Mount the two clamping blocks onto the bear­ing blocks of the microtome:
The bearing blocks of the microtome must be clean. - Clean, if necessary. Place the clamping blocks onto the bearing blocks from above. Do not slide them onto the bearing blocks to prevent the bearing surface from get­ting scratched.
The slot in each of the two clamping blocks must point forward. The left clamping block (1) bears the clearance angle scale (5).
2. Tighten the rear clamping screws (4) of both clamping blocks (Allen key, size 8).
Each cylindrical holder is equipped with two locking pins (6) to prevent the cylindrical holders from sliding out of the clamping blocks.
3. Insert the left cylindrical holder (3) into the left clamping block (1) from the inside outwards, until the outer locking pin (6) is released.
Fig. 59
50
Inserting knives of up to 22 cm length
6
4. Insert right cylindrical holder (3) into the right clamping block (2) from the inside outwards,
7
until outer locking pin (6) is released.
5. Pull both cylindrical holders (3) far enough outwards so that the knife can be inserted easily from the center.
6. Turn the clamping slots (7) upwards to the top.
Put on cut-resistant gloves (see also chapter. 8.1 ‘Additional accessories’).
Instruction manual V 1.3 – 10/2007
Page 51
3311
Fig. 60
8
11
10
5. Operation
7. Insert the knife (11), from the center outwards,
into the left cylindrical holder (3). d-Profile knives: facet must point backwards. c-Profile knives: can be inserted either way.
8. Slide the right cylindrical holder (3) over the
knife - support the back of the knife from be­low with one of your hands.
9. Slide both knife guards (10) over the knife edge.
10. Turn the knife forward far enough to be able
to easily access knife clamping screws (9).
11. Secure the knife holder in that position tight-
ening one of knife clamping screws (8).
8
9
12. Tighten knife clamping screws (9) on both sides
(with Allen key size 8; tighten screws alternately and only as many as the knife is actually wide).
13. Release clamping screw (8).
14. Select the desired clearance angle (5) (see
page 47 for details on clearance angle scale).
15. Sustain the knife holder in the desired clear-
ance angle position and tighten front clamp­ing screws (8) alternately (Allen key size 8).
5
Fig. 61
Fig. 62
Leica SM2500
Inserting knives of 30 cm length
Put on cut-resistant gloves (see also chapter. 8.1 ‘Additional accessories’).
4. Pull the left cylindrical holder (3) all the way
outwards and turn the clamping slot (7, Fig. 59) upwards to the top.
Right cylindrical holder (3) is not yet inserted.
5. Guide the knife through the right clamping
block (2) from the outside inwards.
6. Support the knife from below.
7. Slide the right cylindrical holder (3) onto the
knife, from the outside inwards through the right clamping block (2).
32
8. Continue from point 9 above onwards.
51
Page 52
5. Operation
5
3a
Fig. 63
4
6
1
8

5.5.10 Mounting knife holder C

5
3
4
6
2
7
5
Knife holder C consists of 3 components: The knife holder with integrated knife guard and
two clamping blocks to fasten the knife holder to
3a
the microtome.
1. Preassemble the three components prior to mounting them onto the microtome:
Slide the clamping blocks (1, 2) onto the pins (3a) of the knife holder (3). Clamping block 1 (bearing the clearance angle scale) must be on the left, clamping block (2) on the right; the slots in each clamping block must point backwards. Secure with two slotted screws (4).
The bearing blocks of the microtome must be clean. - Clean, if necessary. Place the preassembled knife holder onto the bearing blocks from above. Do not slide the knife holder onto the bearing blocks to prevent the bearing surface from getting scratched.
Fig. 64
52
2. Place the knife holder onto the two bearing blocks of the microtome, fitting the two pins (one on the underside of each clamping block) into the bores in the bearing blocks.
3. With an Allen key, size 8, tighten all 8 clamp­ing screws (5, 6) lightly and alternately.
4. Next, loosen the 4 rear screws (5) again.
Tightening first all 8 screws followed by releasing the 4 rear screws is im­portant to prevent the knife holder from being misaligned!
5. Turn the knife holder (3) upwards/backwards until the knife clamping slot is in the position shown in Fig. 64 and the 6 knife clamping screws (8) are easily accessible.
Instruction manual V 1.3 – 10/2007
Page 53
5. Operation
8
5
If the knife holder cannot be turned, it has been misaligned. - In that case, loosen all 8 clamping screws and re­tighten as described on page 50.
6. Retighten any one of the rear clamping screws
(5) to secure the knife holder in that position.
Prior to inserting the knife always tighten one of the rear clamping screws to secure the knife holder position!
Put on cut-resistant gloves (see also chapter. 8.1 ‘Additional accessories’).
7
7. Insert the knife with the writing pointing up-
wards (i.e. pointing backwards when being inserted).
8. Tighten knife clamping screws (8) with an Allen
key size 8 starting with the center screws and working your way outwards from side to side. Only tighten as many screws as the knife is actually wide.
Do not misalign the knife when tight­ening the knife clamping screws (8). This will negatively affect sectioning results!
Leica SM2500
9. Place knife guard (7) over the knife edge.
10. Loosen rear clamping screw (5).
11. Turn the knife holder into the desired clearance
angle position (see p. 47 for details on the clear­ance scale) and hold it in that position.
12.Lightly retighten one of the rear clamping
screws and let go of the knife holder.
13. Tighten remaining clamping screws (5) alter-
nately and lightly.
14. Tighten all clamping screws (5, 6) alternately.
53
Page 54
5. Operation
10 11
Fig. 66
8
Fig. 67
12
12
12
Adjusting the knife level (knife holder C)
With each resharpening procedure the overall height of the knife diminishes.
Therefore, the adjusting screws (12) may have to
8
be readjusted from time to time to ensure the knife is always situated at the correct level.
Correct knife level:
There has to be a 3-mm distance between the lower edge (10) of the wedge angle and the up­per edge (11) of the pressure plate of the knife
5
holder. To adjust the knife level:
Insert knife into holder and clamp.
Turn the knife holder so that the knife edge points upwards.
Tighten one of the rear clamping screws (5) to secure the knife holder in that position.
Release the knife clamping screws (8).
Readjust the adjusting screws (12) using an Allen key, size 4, until the distance between lower edge (10) and upper edge (11) is 3 mm.
Retighten the knife clamping screws (8) with an Allen key size 8 starting with the center screws and working your way outwards from side to side. Only tighten as many screws as the knife is actually wide.
Continue from point 9 (page 51) onwards.
54
Instruction manual V 1.3 – 10/2007
Page 55
5. Operation

5.5.11 Fixed-setting declination blocks (45°) and knife holder A or C

When knife holder A or C is combined with the declination blocks, the knife guard (9) cannot be placed over the knife edge. Therefore, use extreme caution when working with this con­figuration - wear cut-resistant gloves!
Installing knife holder A
Place left declination block (1) (bearing the
6
9
4
6
3
3a
5
clearance angle scale (7)) onto the left bear­ing block of the microtome and secure with 3 screws (4) (Allen key size 8) .
The bearing surfaces must be clean. Place the declination blocks onto the bearing blocks from above. Do not slide them onto the blocks to prevent the bearing surface from getting scratched.
73a 81
Leica SM2500
2
Fig. 68
Slide the left pin (3a) of knife holder A into the
declination block (1).
Slide right declination block (2) onto right pin
(3a) of the knife holder and fasten to the mi­crotome with 4 screws (5) (Allen key size 8).
Tighten one of the Allen screws (6) with an Allen key size 8 to secure the knife holder in position! Put on cut-resistant gloves (see also chapter. 8.1 ‘Additional accessories’).
Insert the knife into the holder from below
(with the facet pointing downwards).
The combination ‘Declination blocks / knife holder A’ can only be used for knives up to max. 22 cm length.
55
Page 56
5. Operation
Clamping the knife: Tighten the clamping screws (8, Fig. 68) (Allen key size 8) starting with the center screws and working your way outwards from side to side. Only tighten as many screws as the knife is actually wide.
Make sure not to misalign the knife when tightening the knife clamping screws (8, Fig. 68) as this will nega­tively affect sectioning results!
Loosen Allen screw (6) (Allen key, size 8).
Turn the knife holder into the desired clear­ance angle position (7).
Hold the knife holder in that position an tighten both Allen screws (6) (Allen key, size 8).
56
3a
6
7 1
4
9
6
53a
Installing knife holder C
Place left declination block (1) (bearing the clearance angle scale (7)) onto left bearing block of the microtome and secure with 3 screws (4) (Allen key size 8) .
The bearing surfaces must be clean. Place the declination blocks onto the bearing blocks from above. Do not slide them onto the blocks to prevent the bearing surface from getting scratched.
Slide the left pin (3a) of knife holder C into the declination block (1).
2
Fig. 69
Slide right declination block (2) onto right pin (3a) of knife holder C and fasten to the micro­tome with 4 screws (5) (Allen key size 8).
Instruction manual V 1.3 – 10/2007
Page 57
5. Operation
Fig. 70
8
until the clamping slot is in the position shown in Fig. 70 and the 6 knife clamping screws (8) are easily accessible.
Tighten one Allen screw (6, Fig. 69) (Allen key
size 8) to secure the knife holder in that position.
Turn the knife holder (3) upwards/backwards
Prior to inserting the knife, always tighten one of the two Allen screws (6, Fig. 69) to secure the knife holder in position!
Put on cut-resistant gloves (see also chapter. 8.1 ‘Additional accessories’).
• Insert the knife into the knife holder from
above, with the writing pointing upwards (i.e. pointing backwards when being inserted). The knife back (base of the knife) must fit against the bearing surface inside the knife holder.
Tighten the clamping screws (8) with an Allen
key size 8, starting with the center screws and working your way outwards from side to side. Only tighten as many screws as the knife is actually wide.
Leica SM2500
Do not to misalign the knife when tightening knife clamping screws (8)!
Loosen Allen screw (6).
Select the desired clearance angle (7) turn-
ing the knife holder (to turn, hold the knife holder by the cylindrical pins, not by the knife edge!)
• Hold the knife holder in that position and
tighten both Allen screws (6).
57
Page 58
5. Operation

5.5.12 Working with fixed-setting (45°) declination blocks and knife holder B

Steps 1 - 4 for all knife lengths:
1. Place left declination block (1) (with clearance angle scale (5)) onto left microtome bearing block and secure with 3 screws (6) (Allen key size 8).
The bearing surfaces must be clean. Place declination blocks onto bearing blocks from above - do not slide them onto the bearing blocks to prevent the bearing surfaces from getting scratched.
12
10
3
9
12
6
8
11
2. Insert the left cylindrical holder (3) (marked by a black line) from the in- side outwards through declination block (1) until the left locking pin (10) is released.
3. Pull the left cylindrical holder (3) outwards as far as it will go and turn it upwards until clamping slot (12) is located at the top; secure with Allen screw (8) using an Allen key size 8.
4. Place the right declination block (2) onto the right bearing block of the microtome and fasten with 4 screws (7) (Allen key size 8).
1
5
Fig. 71
Inserting knives up to a length of 22 cm:
5. Insert the right cylindrical holder (4) from the inside outwards through the right declination block (2) until the right locking pin (10) is released.
8
7
6. Pull cylindrical holder (4) outwards as far as it will go.
Put on cut-resistant safety gloves (see also chapter 8.1 ‘Additional accessories’).
7. Insert the knife, from the center outwards, into the left cylindrical holder (3).
8. Slide the right cylindrical holder (4) onto the knife - support the knife from
10
4
2
9
below with one of your hands.
9. Slide knife guards (11) right and left over the knife edge.
10. Loosen Allen screw (8) on the left declination block.
11. Turn the knife forward until screws (9) are easily accessible.
12. Re-tighten Allen screw (8) on the left declination block (Allen key size 8).
11
13. Tighten the knife clamping screws (9) on both cylindrical holders with an Allen key size 8 (alternately and only as many as the knife is wide).
Fig. 72
14. Loosen Allen screw (8).
15. Turn the knife holder into desired clearance angle position (5), sustain it in that position and tighten both Allen screws (8) (Allen key size 8).
58
Instruction manual V 1.3 – 10/2007
Page 59
5. Operation
Inserting knives up to a length of 30 cm
Put on cut-resistant safety gloves (see also chapter 8.1 ‘Additional accessories’).
Steps 1 through 4 - see previous page.
5. Insert the knife into the left cylindrical holder (3) guiding it through the
right declination block (2) from the outside inwards.
6 Slide the right cylindrical holder (4) from the outside inwards through the
right declination block (2) and onto the knife - support the knife from be­low with one of your hands.
7. Slide knife guards (11) right and left over the knife edge.
8. Loosen Allen screw (8) on the left declination block (Allen key size 8).
9. Turn the knife forward until the knife clamping screws (9) are easily ac-
cessible.
10. Re-tighten Allen screw (8) on the left declination block (Allen key size 8).
11. Tighten the knife clamping screws (9) on both cylindrical holders with an
Allen key size 8 (alternately and only as many as the knife is wide).
12. Loosen Allen screw (8).
13. Turn the cylindrical holders into the desired clearance angle positon (5).
14. Sustain the knife holder in that position and tighten both Allen screws (8)
(Allen key size 8).

5.5.13 Inserting the disposable blade rail (only in knife holder B)

• Insert the blade rail into knife holder B as described for knives up to
22 cm length.
Open the clamping screws on the blade rail (Allen key size 3).
Insert the disposable blade; tighten the blade clamping screws, starting
from the center and working your way outwards from side to side.

5.5.14 Working with distance blocks for sectioning very high specimen blocks

Place the distance blocks onto the bearing surfaces of the microtome,
then place the regular clamping blocks on top.
Insert the extra-long fitting bolts through both blocks and tighten with an
Allen key, size 8.
Leica SM2500
59
Page 60
5. Operation

5.6 Working with the instrument

The instrument may only be operated by skilled laboratory person­nel. It may only be used for the designated application and oper­ated in accordance with the instructions contained in this manual!
Always wear protective glasses when sectioning brittle speci­mens! - Risk of splintering!
No liquids may enter the interior of the instrument during opera­tion!
Always cover the knife edge with the knife guard during work breaks!
Always remove the knife from the knife holder when finishing work!

5.6.1 Switching the instrument on / selecting parameters

Switch the instrument on (see chapter 5.2).
Select manual mode of operation ‘MAN’ (see chapter 5.4.3).
Mount accessories (specimen, knife holder, knife) (see chapter 5.5).
60
Be sure to comply with all safety instructions provided in chapter
5.5.6 on how to handle microtome knives properly!
Orient the specimen, if needed (see chapter 5.5.5).
Select the sectioning / milling window (see chapter 5.4.14).
Select a stop or photo position (see chapter 5.4.8).
Instruction manual V 1.3 – 10/2007
Page 61

5.6.2 Trimming

5. Operation
Trimming can be done in modes of operation AUTO or PROG. Trimming in AUTO mode of operation:
Preparatory steps:
Activate AUTO mode of operation (see chapter 5.4.3).
Select the desired section thickness (see chapter 5.4.5).
Activate retraction / select retraction setting as needed (chapter 5.4.5).
Select sectioning / milling and return stroke speed (see chapter 5.4.7).
• Select a sectioning mode (see chapter 5.4.6) (for trimming usually
CONT).
Turn the knife guard away from the knife edge.
Start the trimming procedure:
Press RUN STOP + RUN ENABLE (see chapter 5.4.10) or press the foot switch (see chapter 5.4.1).
Trim the specimen down to the desired level.
Press RUN STOP or RUN ENABLE or once again press the foot switch to
stop trimming.
Leica SM2500
Trimming in PROG mode of operation Preparatory steps:
Activate PROG mode of operation.
The difference between PROG and AUTO is that in PROG none of the three sectioning modes (area 2.3, chapter 5.4.6) is selected, but a programmed number of sections is carried out.
Select section thickness, retraction (if needed) and speed as described
for AUTO mode of operation.
• Select the desired number of sections ‘n’ you wish to be carried out
(area 2.2 chapter 5.4.5).
Start the trimming procedure:
Press RUN STOP + RUN ENABLE or press the foot switch:
the instrument carries out the preselected number of sections and then stops in the selected stop or photo position.
61
Page 62

5.6.3 Sectioning

5. Operation
Sectioning can be done in modes of operation AUTO or PROG. Sectioning in AUTO mode of operation: Preparatory steps:
As described for trimming (see page 59).
Select section thickness and speed according to the type of specimen being sectioned.
Select a sectioning mode (see chapter 5.4.6). Sectioning can be done in either CONT, SINGLE or STEP mode of operation.
Starting sectioning:
Press RUN STOP + RUN ENABLE or press the foot switch.
In SINGLE mode, the instrument will carry out one single step and then stop in the stop or photo position selected.
• In STEP mode, the instrument will continue sectioning as long as RUN STOP + RUN ENABLE or the foot switch are being held.
To stop sectioning in CONT mode: Press RUN STOP or RUN ENABLE or press the foot switch once again.
62
Sectioning in PROG mode: Preparatory steps:
Proceed as described for trimming.
If necessary, select sectioning thickness and speed appropriate for the type of specimen being sectioned.
Enter the desired number of sections ‘n’ you wish to be carried out (area
2.2 chapter 5.4.5).
Start sectioning:
Press RUN STOP + RUN ENABLE or press the foot switch: The instrument will carry out the number of sections programmed and
then stop in the defined stop or photo position.
Instruction manual V 1.3 – 10/2007
Page 63

5.7 Switching the instrument off

Wait for the sectioning procedure to be finished (in PROG mode) or stop sectioning by pressing RUN STOP or RUN ENABLE or the foot switch.
If necessary, activate MAN mode of operation and move the sledge to the resting position desired.
Remove the knife.
5. Operation
Wear cut-resistant safety gloves when inserting / removing the knife (see also chapter 8.1 ‘Additional accessories’).
Always remove the knife from the knife holder when finishing work!
Handle microtome knives and blades with utmost care. Knife and blade edges are extremely sharp and can cause severe injuries!
Never leave knives / disposable blades or knife holder with a knife / disposable blade inserted lying around!
Leica SM2500
Always store knives in the knife case when not in use!
Never place a knife on a bench with the edge facing upwards!
Never try to catch a falling knife or disposable blade!
Remove the specimen.
Switch off the mains switch (I/0 - located at rear of control unit, position ‘0’ = Off).
63
Page 64

6. Trouble shooting

6.1 Error codes

In the event of malfunctions, the instrument displays corresponding error codes, described below.
The error codes are shown in the lower display in area 4 (see chap. 5.4.12).
Press any button to acknowledge / delete the error code displayed.
Error code
E 001
E 002
Cause
Connection to limit switches interrupted.
RAM error.

6.2 Instrument / accessories not working

Error
Instrument not working.
Lamp not working.
Vacuum cleaner not working (SP2600).
Cause
Connecting cables / power cord not connected / not correctly connected. Line fuses defective.
Cable not properly connected. Lamp fuse (in control unit) defective.
Cable not properly connected. Vacuum cleaner fuse (in control unit) defective.
Corrective action
Verify if plug screws of connect­ing cable ‘microtome to control unit’ are properly tightened. Call Leica Technical Service.
Corrective action
Verify if connecting cable ‘micro­tome to control unit’ and/or mains cable are properly connected. Replace line fuses. (see chapters 7.2.2 and 4.5.1). Check lamp connecting cable. Replace lamp fuse. (see chapter 7.2.2).
Check vacuum cleaner connect­ing cable. Replace vacuum cleaner fuse. (see chapter 7.2.2).

6.3 Power failure

64
If there is a power failure while the microtome is in motion, the micro­tome will stop in the position where the power failure occurred.
Once power has been restored, the instrument will automatically go to STANDBY mode:
press the STANDBY button to change from standby to active mode.
Instruction manual V 1.3 – 10/2007
Page 65

7.1 Cleaning / disinfection

7. Cleaning, disinfection and maintenance

Prior to any cleaning, disinfection or maintenance work, the in­strument must be switched off and disconnected from mains! Exception: Cleaning or disinfection procedures which require the sledge to be moved.
Remove both knife and specimen prior to any cleaning, disinfec­tion or maintenance work! Wear cut-resistant safety gloves to remove the knife (see chapter
8.1 ‘Additional accessories’).
No liquids may enter the interior of the instrument during clean­ing, disinfection or maintenance! Cleaning only with a slightly moistened cleaning cloth! Disinfection only with disinfectants that allow application with a slightly moistened cloth!
Do not use solvents (e.g. xylene, acetone), cleaners containing solvents or scouring powders for cleaning!
Leica SM2500
Appropriate cleaners for the control unit / control panel and all painted surfaces: mild laboratory detergent (slightly moistened cloth)!
Clean the control panel only in standby mode or when the instru­ment is switched off.
Appropriate cleaners for non-varnished metal parts (knife holders, base plates, specimen clamps): xylene substitutes, alcohol - apply with an only slightly moistened cleaning cloth!
Use cleaners and disinfectants according to the safety instruc­tions of the manufacturer and in compliance with the applicable regulations in your laboratory!
Regularly check and empty the section waste trays located below the extendible carrying handles.
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7. Cleaning, disinfection and maintenance
Cleaning steps:
2
Use a brush to remove all loose sectioning waste from the instrument and dispose of.
Pull out the sectioning waste trays (1) located below the extendible car­rying handles at the front and rear of the instrument.
1
Fig. 73
1
The microtome housing consists of several plastic panels. The front and rear panel are spring-held and can be removed eas­ily when necessary. The waste trays, located below the extendible carrying handles at the front and rear of the housing, gather the sectioning waste which is transferred to the trays via the cover ribbon. The trays must be emptied before they are filled to the brim - other­wise the cover ribbon could be damaged or get dirty. Check the waste trays daily and empty as required.
• Remove the knife holder. Clean the bearing surfaces of the bearing blocks and the knife holder itself.
Remove the upper front panel (2).
Switch the instrument on.
With a brush and/or a cleaning cloth slightly moistened with laboratory cleaner, clean the cover ribbon in the area where it is supported by the deflection roller:
Move the ribbon step by step (via buttons 56 - 59, see area 5.2, chapter
5.4.14) until the entire ribbon has been cleaned.
If possible, clean the cover ribbon only in the area where it is sup­ported from below by the deflection roller. Only very light pressure may be applied to the horizontal part of the ribbon - otherwise it might slip out of the lateral guides. Should that happen, immediately re-insert the ribbon into the guide(s) to prevent sectioning waste from entering the interior of the instrument!
66
Put the upper front panel (2) back in place.
If required, wipe down the housing surfaces with a cleaning cloth slightly moistened with laboratory cleaner.
Instruction manual V 1.3 – 10/2007
Page 67

7.2 Maintenance

7. Cleaning, disinfection and maintenance
Disinfection
• If you work with potentially infectious material the instrument
disinfected accordingly.
Slightly moisten a cleaning cloth with disinfectant and disinfect the in-
strument as per the instructions of the manufacturer of the disinfectant used.
Only authorized service technicians may access the internal com­ponents of the instrument for service and repair!
Prior to any cleaning, disinfection or maintenance work, the in­strument must be switched off and disconnected from mains!
Users may only carry out the maintenance work described in chapters 7.2.1 and 7.2.2! ­Any other maintenance work must be carried out by authorized Leica service technicians.
must be

7.2.1 General maintenance steps

• At least once a year, have the instrument inspected by an authorized
Leica technical service engineer.
Enter into a service contract once the warranty period has expired.
Clean the instrument daily (or every time after use).
• Regularly remove dust from the air ducts at the rear of the instrument
using a brush or a vacuum cleaner.
Leica SM2500
No liquids may enter the interior of the instrument during cleaning, disinfection or maintenance!
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7. Cleaning, disinfection and maintenance

7.2.2 Replacing the fuses

Prior to exchanging any fuse, always switch the instrument off and unplug the mains plug. Defective fuses may be replaced only with the replacement fuses supplied together with the instrument or with other replacement fuses of exactly the same type / specification! Important! Follow the instructions below to make sure you select the correct replacement fuse for each fuse holder.
Selecting the correct replacement fuse
The amp value (here 10A) printed on the fuse and the amp value next to the fuse holder on the rear panel of the instrument MUST be identical!
Fig. 74
Fuse values are shown on the metal caps of the replacement fuses - the fuse imprint looking more or less as shown on the left (may vary slightly depending on fuse type).
Amperage (A) is the parameter that is important for allocating fuses correctly to the correspond­ing fuse holders (shown in the example on the left: line fuse 2xT10A ).
Two fuse holders are located on the rear panel of the instrument - see Fig. 76
on the left. The top
imprint ‘line fuse’ refers to the line fuses (2xT10A) in the voltage selector (Fig. 77).
Check the instrument malfunction table (chap. 6.2 ‘Instrument / accessories not working’) to find out which fuse needs to be replaced.
Example: Instrument not working --> possibly the line fus-
es 2xT10A (Fig. 77) need to be exchanged; i.e.
select the correct replacement fuses (10A) as per the instructions on this page.
Replacing the fuses: --> proceed to next page.
Fig. 75
68
Instruction manual V 1.3 – 10/2007
Page 69
Replacing the vacuum cleaner or lamp fuse
7. Cleaning, disinfection and maintenance
3
1
2
Fig. 76
Insert a screwdriver into slot (1); push fuse holder (2) slightly inwards turning at the same time by 1/4 turn to the left.
The fuse holder is released and can be re­moved.
Replacing the line fuses
To replace the line fuses, proceed exactly as per chapter 4.5.1 ‘Verifying and adjusting the voltage selector’.
Do not, however, change the orientation of the voltage selector, but simply replace the defec­tive fuses by new ones of correct specifica­tion.
2
Fig. 77
Remove the defective fuse (3) from the fuse holder (2) and replace by a replacement fuse of correct specification.
Reinsert the fuse holder / fuse into the instru­ment. Use the screwdriver to push slightly in­wards, turning at the same time by 1/4 turn to the right.
Caution! After replacing the line fuses and rein­serting the voltage selector housing into the instrument, double-check whether the correct voltage selector setting is visible.
Leica SM2500
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7. Cleaning and maintenance
3
Fig. 78
2
1
Fig. 79

7.2.3 Lubricating the bearing points and the nut of the micrometer spindle

Under normal operating conditions it is sufficient to lubricate the bearing points and the nut of the micrometer spindle after approx. every 250 hours of service:
Switch the instrument on (MAN mode - see
chapter 5.4.3).
Remove specimen holder and base plate from
the sledge.
Remove cover (3):
the first lubrication point (1) becomes visible.
Fill the grease gun (standard delivery) and ex-
tract the air from the gun (press several times on a cleaning cloth or piece of paper until grease comes out).
Place the grease gun onto the first lubrication
point and press once or twice.
Via the manual sledge movement (area 5.2,
chapter 5.4.14) move the sledge backwards until the second lubrication point (2) becomes visible through the round opening in the cover ribbon.
Place the grease gun onto the second lubri-
cation point and press once or twice.
If required, move the sledge back forward as
far as needed (manual sledge movement, area
5.2, chapter 5.4.14).
Put cover (3) back in place.
Re-insert the base plate and specimen holder
(see chapter 5.5.1).
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Instruction manual V 1.3 – 10/2007
Page 71

8. Overview - accessories

8.1 Knife holders, specimen holders, additional accessories

Knife holders and knife holder accessories
Knife holder A
Knife holder B
Knife holder C
Pair of declination blocks - fixed declination angle of 45°, for knife holders A, B and C
• Pair of distance blocks including set of extra-long fitting bolts, block height 62 mm; usable with knife holders A, B and C
Specimen holders (base plates, specimen and specimen stage holders)
Base plate with vise, 80 x 100 mm
Mountable jaws for vise 80 x 100 mm, for sectioning very high specimen blocks
Base plate with dovetail guide (including eccentric ring).
Eccentric ring for base plate (part of standard delivery of ‘base plate with dovetail guide’; to be ordered separately for use with ‘base plate with vise’)
Leica SM2500
The eccentric ring blocks the orientation mechanism to ensure the sectioning plane is not altered. However, when using the eccentric ring the specimens CANNOT be oriented.
Specimen clamp, 44 x 58 mm (to be fastened on base plate with dovetail guide)
Round specimen holder for specimens of 6, 15 and 25 mm diameter (to be fastened on base plate with dovetail guide)
Vacuum stage adapter (to be fastened on base plate with dovetail guide)
The vacuum stages are above all used for milling applications (Leica SP2600 Ultramilling Attachment), though in some cases, vacuum stages might be suitable for Leica SM 2500 sectioning ap­plications. - Please contact your Leica representative if you need any further information.
71
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8. Overview - accessories
Preconfigured specimen holder sets
Base plate with dovetail guide, including eccentric ring and specimen clamp 44 x 58 mm
Base plate with dovetail guide, including eccentric ring and holder for round specimens of 6, 15 and 25 mm diameter.
Specimen stages
• Metal specimen stages with special surface texture for paraffin embeddings, available in the following sizes:
- 8 x 6.5 cm
- 12 x 8 cm
- 16 x 12 cm
- 25 x 20 cm
Plastic specimen stages for celloidin embeddings, available in the fol­lowing sizes:
- 8 x 6.5 cm
- 12 x 8 cm
- 20 x 12 cm
- 25.5 x 19 cm
72
Embedding frame
Aluminum embedding frame, adjustable in size, fits all specimen stage sizes available, for block sizes up to 200 x 260 mm.
Additional accessories
Safety gloves, cut-resistant - for safe handling of the knives.
Instruction manual V 1.3 – 10/2007
Page 73
8. Overview - accessories

8.2 Overview - which knife to be used in which knife holder

0052MSrüfresseM-thcisrebÜ
pytresseMremmunelieTAretlahresseMBretlahresseMCretlahresseM
°04lekniwlieK,egnäLmc21774216120x °05lekniwlieK,egnäLmc21874216120x °06lekniwlieK,egnäLmc216 °07lekniwlieK,egnäLmc218737116120x °04lekniwlieK,egnäLmc61937116120x
ressemllatemtraH
1311066120x
ressemlhatS
-negnilkgewniE
neneihcs
°05lekniwlieK,egnäLmc6104711612
°06lekniwlieK,egnäLmc61147116120x cCliforP,egnäLmc21411066120x dCliforP,egnäLmc21521066120x cCliforP,egnäLmc6 dCliforP,egnäLmc61511066120x
°04lekniwlieK,egnäLmc21727116120x
°05lekniwlieK,egnäLmc21827116120x
°04lekniwlieK,egnäLmc61037116
°05lekniwlieK,°04,egnäLmc61137116120x
°04lekniwlieK,°04,egnäLmc22337116120x
°05lekniwlieK,egnäLmc22437116
cCliforPegnäLmc21821066120x dCliforPegnäLmc21721066120x
cCliforP,egnäLmc61670066120x dCliforP,egnäLmc6187006
cCliforP,egnäLmc22770066120x
dCliforP,egnäLmc22970066120x
bliforP,egnäLmc22411706120x
cliforP,egnäLmc226117
dliforP,egnäLmc22631706120x
bliforP,egnäLmc03621706120x
cliforP,egnäLmc03821706120x dliforP,egnäLmc0393170
relartneztimretlahnegnilkgewniE
negnilkgewniE-dnablamhcSrüf,ettalpkcurdnA
37116120x
0x
120x
120x
6120x
06120x
6120x
577
238630x
Leica SM2500
All knives are delivered in a special knife storage case.
73
Page 74

9. Warranty and service

Warranty
Leica Biosystems Nussloch GmbH guarantees that the contractual product delivered has been subjected to a comprehensive quality control procedure based on the Leica in-house testing standards, and that the product is fault­less and complies with all technical specifications and/or agreed character­istics warranted.
The scope of the warranty is based on the content of the concluded agree­ment. The warranty terms of your Leica sales organization or the organiza­tion from which you have purchased the contractual product shall apply exclusively.
Service information
If you are in need of technical customer service or spare parts, please con­tact your Leica representative or the Leica dealer where you purchased the instrument.
Please provide the following information:
Model name and serial number of the instrument.
Location of the instrument and name of the person to contact.
Reason for the service call.
Delivery date.
Decommissioning and disposal
The instrument or parts of the instrument must be disposed of according to existing local applicable regulations. Special attention should be paid to the lithium cell of the electronic circuit board!
74
Instruction manual V 1.3 – 10/2007
Page 75
10. EC Declaration of Conformity
EC Declaration of Conformity
We herewith declare, in exclusive responsibility, that the instrument
Leica SM2500 – Sliding Microtome
with and without SP2600 – Ultramiller Attatchment
was developed, designed and manufactured to conform with the
• Council Directive 73/23/EEC, (Low Voltage) and
• Council Directive 89/336/EEC, Appendix I (Electromagnetic Compatibility),
including their amendments up to the date mentioned below.
The following harmonized standards were applied:
• EN 61010-1: 2001
Safety requirements for electrical equipment for measurement, control and laboratory use ­Part 1: General requirements
• EN 61326-1:1997 + EN 61326/1:1998
Electrical equipmentl for measurement, control and laboratory use - EMC requirements ­Part 1: General requirements
• EN 61000-3-2:1995 + A1:1998 + A2:1998 + A14:2000
Electromagnetic compatibility (EMC) Part 3-2: Limits - Limits for harmonic current emissions
• EN 61000-3-3:1995
Electromagnetic compatibility (EMC) Part 3: Limits ­Section 3: Limitation of voltage fluctuations and flicker in low-voltage supply systems for equirement with rated current £ 16 A
• EN 418:1992
Emergency stop equipment. Principles for design.
• EN 1037:1995
Safety of machinery. Prevention of unexpected start-up.
In addition, the following in-house standards were applied:
• DIN EN ISO 9001: 2000.
Leica Biosystems Nussloch GmbH Postfach 1120 D-69222 Nussloch
October 11, 2007
. . . . . . . . . . . . . . . . . . . . . . . . . . . .
Anne De Greef-Safft
President Biosystems Division
Page 76
11. Peoples Republic of China
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Instruction manual V 1.3 – 10/2007
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